JournalTOCs

Bulletin of Experimental Biology and Medicine
Journal Prestige (SJR): 0.268

Citation Impact (citeScore): 1
Number of Followers: 0

Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1573-8221 - ISSN (Online) 0007-4888
Published by Springer-Verlag

[2626 journals]

Induced Pluripotent Stem Cell-Derived Hematopoietic Embryoid Bodies
Improve Mouse Status in Septic Peritonitis
- Abstract: We examined the efficacy of embryoid bodies from 6-day induced pluripotent stem cells an in vivo sepsis model. Injection of embryoid bodies to septic mice improved the condition of their lungs and significantly increased their survival rate. Although embryoid bodies secretedsphingosine-1-phosphate in vitro, its serum levels in mouse plasma were significantly reduced compared to that in the control (untreated mice receiving PBS). Low concentrations of sphingosine-1-phosphate protected endothelial cells, while high concentrations disrupted endothelial barrier integrity. Therefore, exogenous sphingosine-1-phosphate secreted by embryoid bodies during early stage of sepsis might down regulate endogenous production of sphingosine-1-phosphate. Inhibition of excessive sphingosine-1-phosphate release protects against endothelial injury and suppresses a vicious cycle of inflammatory reactions. The obtained results open new prospects in induced pluripotent stem cells-based therapy for sepsis.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04414-2
Evaluation of Lipid Peroxidation—Antioxidant Defense System Depending on
the Stage of Stress Reaction in Hypertensive ISIAH Rats
- Abstract: The stages of stress reaction (anxiety and resistance) were reproduced on the model of stresssensitive hypertensive ISIAH rats. Analysis of the experimental data showed an increase in activity of the antioxidant defense system in ISIAH rats: total antioxidant activity of the blood and superoxide dismutase activity increased at the stage of anxiety and then decreased during the resistance stage.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04402-6
Death Mechanisms of Pulmonary Alveolocytes in Mice Infected with Influenza
- Abstract: In CBA mice infected with influenza viruses A/H1N1/California/04/2009 and A/H5N1/Goose/Krasnoozerskoye/627/05 in a dose of 10 MLD50, the mechanisms of death of pulmonary alveolocytes over 10 postinfection days were studied by light microscopy, immunohistochemistry, and morphometry. In mice infected with A/H1N1, alveolocytes died predominantly via necrosis, while apoptosis mostly employed the mitochondrial pathway. In mice infected with A/H5N1, apoptosis was the dominant mechanism of alveolocyte death proceeded via membrane receptor signaling followed by switching to FAS-mediated pathway via activation of FADD, the apoptotic signal transduction protein.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04408-0
Comparison of Reactivity of the Lipid Peroxidation–Antioxidant Defense
System in Normal and Hypertensive Rats at Different Stages of
Stress-Reaction
- Abstract: We compared changes in the parameters of LPO—antioxidant defense system at various stages of the stress response in mature male ISIAH rats (hereditary stress-induced arterial hypertension) and WAG rats in 3 h (stage of anxiety) and 7 days (resistance stage) after a 3-h single immobilization in the supine position. It was found that post-stress reactivity of the nonspecific stress-limiting LPO system is different in normotensive and hypertensive rats. ISIAH rats, irrespective of the stage of the stress-reaction, demonstrated more intense LPO reactions (accumulation of TBA-reactive products) and reduced antioxidant defense parameters (low content of lipid-soluble vitamins, increased oxidized glutathione content) in comparison with the corresponding parameters in WAG rats.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04403-5
Study of the Biocompatibility of Titanium-Niobium Implants by the
Parameters of Their Osseointegration under Experimental Conditions
- Abstract: Superelastic titanium alloys (titanium-niobium-zirconium and titanium-niobium-tantalum) are considered to be the more advantageous choice for dental implants than titanium alloys due to their physico-mechanical properties close to those of the bone tissue. Morphological studies of the biocompatibility of these alloys in comparison with titanium were carried out in vivo. Scanning electron microscopy and microprobe elemental analysis of the tissue along the interface between the bone and titanium/superelastic titanium alloys showed similar morphology and elemental composition both on days 30 (poorly mineralized connective tissue was detected in sites of contact with titanium alloy samples) and 90 after surgery (the surface of the titanium alloy samples was covered with mineralized tissue similar by its composition to the surrounding bone).
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04418-y
Structural Analysis of the Myocardium in Experimental
- Abstract: We studied dynamic changes in the total number of cardiomyocytes and the character of structural lesions in the myocardium in rats with modeled anthracycline-induced cardiomyopathy provoked by a single injection of doxorubicin in a dose of 10 mg/kg alone or in combination with subsequent adrenergic stimulation. The injections of epinephrine during the development of anthracycline-induced cardiomyopathy resulted in more pronounced loss of body weight, stronger decrement of the heart weight, and more severe decrease of the cardiomyocyte count in comparison with the corresponding changes induced by doxorubicin alone. The basic lesions of cardiomyocytes in anthracycline-induced cardiomyopathy are the lytic alterations and subsegmental contractures; in contrast, combined use of doxorubicin and epinephrine provoked degree II and III contractures. The revealed necrobiotic changes of cardiomyocytes resulted in their death and pronounced decrease of their number at the initial terms of the study. Hypertrophy observed at later terms of the experiments in parallel with partial recovery of cardiomyocyte number reflected the development of regenerative and adaptivecompensatory processes induced by massive death and elimination of the parenchymatous cells (up to 36-37% of population).
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04419-x
Cytokine Profiling of Subclinical Tick-Borne Infections in Humans
- Abstract: Over many years, tick-borne infections remain one of the most serious threats to human health worldwide. The immune response to these infections in a human after confirmed bite by an infected carrier at the early stages of infection in the absence of clinical symptoms can be the first indicator of the presence of the infectious agent in the body. During viral infection, the concentration of IL-1α, IL-8, IL-10, IL-17A, and IFNγ increases; superoxide dismutase also increases, in contrast to bacterial infections. A slight decrease in the concentration is observed only for receptor antagonist IL-1Ra. During the infection caused by bacterial pathogens, very similar profiles of the innate human immune response are observed: activation of IL-1α, IL-8, and IFNα and suppression of superoxide dismutase, IL-1Ra, and IL-17A production. It has been demonstrated, that the immune response is triggered immediately after infection, and changes in the concentration of the main cytokines in the blood plasma can be detected as early as on days 2-5 after tick bite. These results can be useful in developing new methods of emergency diagnosis and prevention of tick-borne infections.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04405-3
Transcriptome Guided Drug Combination Suppresses Proliferation of Breast
Cancer Cells
- Abstract: One of actively developing trends in modern pharmacology is the use of the transcriptome analysis for drug repositioning. We have previously detected two molecular markers of relapses in patients with malignant breast tumors: ELOVL5 and IGFBP6. Poor prognosis is associated with low expression of these markers. Here we analyze the effects of simvastatin and a new potential proteasome inhibitor K7174 inducing expression of IGFBP6 and EVOVL5 on the proliferation of breast cancer cells MDA-MB-231 and DU4475. Compound K7174 potentiates the inhibitory effect of simvastatin on the proliferation of DU4475 cells characterized by low expression of ELOVL5-IGFBP6 pair, but not on the proliferation of MDA-MB-231 cells with high expression of these markers.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04412-4
Structure of Rat Testicles after Intravenous Injection of Nanosized
Magnetite Particles
- Abstract: The structure of the testicles was studied in adult rats in 120 days after a single intravenous injection of chitosan-modified (magnetic nanospheres) and lipid-modified (magnetoliposomes) nanosized magnetite particles. Perls histochemical reaction detected in the testicular interstitial connective tissue the cells which absorbed and accumulated magnetite nanoparticles. The dynamics of spermatogenesis index and the count of Perls+ cells in the rat testicles were traced throughout the experiment. The studied modified nanosized magnetite particles did not penetrate through the blood—testicle barrier in rats.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04417-z
Behavioral and Cognitive Reactions of Rats with Experimental Chronic
Obstructive Lung Diseases of Different Degree
- Abstract: The study presents the results of behavioral phenotyping of rats with experimental chronic obstructive pulmonary disease (eCOPD) of different severity modeled by using a combination of inductors (purified papain and bacterial LPS) in increasing concentrations. Locomotor and exploratory activity, spatial memory, and anxiety were evaluated. In mild eCOPD, locomotor and exploratory activity increased, while other parameters did not differ from the control values. Rats with moderate eCOPD demonstrated moderately restricted locomotor function and increased anxiety. Severe eCOPD was associated with pronounced changes in all analyzed parameters. Correlation analysis revealed relationships between some behavioral parameters and blood oxygen saturation, inspiratory lung volume, and respiration rate. This indicates the role of respiratory failure in the pathogenesis of cognitive disorders.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04400-8
Possibilities of Postmortem Magnetic Resonance Imaging for Evaluation of
Anasarca in Newborns
- Abstract: We studied the possibilities of postmortem magnetic resonance imaging (MRI) for evaluation of anasarca in newborns. Before the autopsy, MRI in T1 and T2 standard modes was performed, signal intensity tomograms in the subcutaneous tissue were analyzed, and tissue hydration index was calculated. Using 3D reconstruction, we determined the values of hydrothorax, hydropericardium, and ascites, as well as specific volumes of fluid in the abdominal and thoracic cavities. It has been established that postmortem MRI of deceased newborns provides objective quantitative assessment of anasarca, as well as absolute and relative volumes of hydrothorax and ascites, which improves the diagnostic capacities of pathoanatomical autopsy or forensic examination of a corpse and contributes to the determination of tanatogenesis. However, postmortem MRI cannot fully replace traditional autopsy that allows conducting complex macroscopic and microscopic analysis of organs and tissues.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04415-1
Synthetic Phenolic Antioxidant TS-13 Suppresses the Growth of Lewis Lung
Carcinoma and Potentiates Oncolytic Effect of Doxorubicin
- Abstract: ROS are important intracellular messengers; their ambiguous role in malignant processes was demonstrated in many studies. The effects of a synthetic phenolic antioxidant sodium 3-(3’-tert-butyl-4’-hydroxyphenyl)propyl thiosulfonate sodium (TS-13) on the tumor growth and oncolytic properties of doxorubicin were studied in the experimental model of Lewis lung carcinoma in mice. In mice receiving TS-13 with drinking water (100 mg/kg), suppression of tumor growth by 32.3% was observed on day 21 after inoculation of Lewis lung carcinoma cells. Two-fold intraperitoneal injections of doxorubicin in a cumulative dose of 8 mg/kg were followed by inhibition of tumor growth by 49.5%. Combined treatment with TS-13 and doxorubicin suppressed the tumor growth by 55.4%. In contrast to doxorubicin, TS-13 inhibited NO generation by peritoneal macrophages. The results show the prospect of studying TS-13 in the context of overcoming drug-resistance of tumors.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04410-6
Comparison of Profiles of Extracellular MicroRNA Secreted by Caco-2 Cells
from the Apical Side of the Membrane under Static and Microcirculation
Conditions
- Abstract: Extracellular microRNA are one of the indicators of the functional state of cells. Culturing of Caco-2 cells under the conditions of microcirculation in a Homunculus microfluidic device allows better simulating natural environment of the body in comparison with static culturing. Impedance spectroscopy (BioClinicum Research Center) was used for non-invasive estimation of the monolayer quality and changes in the cell apical membrane due to the formation of microvilli. Under static conditions, Caco-2 cells release more microRNA from the apical membrane than under microcirculation conditions, while secretion of miR-320a, miR-24-3p, and miR-221-3p microRNA under static conditions can indicate stress of the cells and activation of inflammatory response. Under microcirculation conditions, the expression of laminin-α1 (LAMA1) was lower than under static conditions, which indicates deeper differentiation of cells.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04406-2
Antimetastatic Activity of Combined Topotecan and Tyrosyl-DNA
Phosphodiesterase-1 Inhibitor on Modeled Lewis Lung Carcinoma
- Abstract: The antimetastatic activity of combined or individual administration of topotecan and tyrosyl-DNA phosphodiesterase 1 (Tdp1) inhibitor was examined under various administration schedules in mice with Lewis lung carcinoma modeled by intravenous injection of 200,000 clone/mouse. The greatest antimetastatic effect was observed after combined use of topotecan and Tdp1 inhibitor as documented by macroscopic study of the lungs that revealed the decreased metastatic scores by 76, 91, or 74% at the respective inhibitor doses of 2, 4, or 6 mg/mouse, respectively, in parallel with inhibition of metastasis up to 98% (at inhibitor dose of 4 mg/mouse) and morphological and morphometric analyses of the lung sections, which revealed elevation of metastasis growth delay index to 86 and 63% at the respective inhibitor doses of 4 and 6 mg/mouse, respectively. The combined administration of topotecan and Tdp1 inhibitor is viewed as the most effective way to eliminate the metastatic formations with possible restitution of focal lesions.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04413-3
Effect of 4-Methyl-Phenyl Biguanide on Free Radical Homeostasis and
Activity of Oxidative Metabolism Enzymes in Rats with Cardiovascular
Pathology Developing against the Background of Rheumatoid Arthritis
- Abstract: We studied the effect of 4-methyl-phenyl biguanide, a substance chosen by PASS software (Prediction of Activity Spectra for Substance), on oxidative status and activity of oxidation metabolism enzymes participating in limitation free radical processes (aconitate hydratase, glucose-6-phosphate dehydrogenase, and NADP-isocitrate dehydrogenase) in the heart and blood serum of rats with cardiovascular pathology developing against the background of experimental rheumatoid arthritis. Activity of cardiomyocyte cytolysis markers in rat blood serum was also measured. Normalization of the studied parameters under the influence of the test compound in animals with experimental pathology attests to its positive effects on metabolic and free radical processes.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04401-7
Expression of Aβ42, τ-Protein, p16, p53 in Buccal Epithelium: Prospects
for Use in the Diagnostics of Alzheimer’s Disease and Rate of Aging
- Abstract: The expression of Aβ42, and τ-protein, and p16 and p53 proteins was analyzed in the buccal epithelium of elderly and senile patients with Alzheimer’s disease. We revealed enhanced synthesis of Alzheimer’s disease markers Aβ42 (by 15-30 times) and τ-protein (by 5 times) in comparison with the corresponding values in people without neurodegenerative pathology of the same age groups. In addition, increased synthesis of proteins of cell aging and apoptosis p16 (by 6-10 times) and p53 (by 2-3 times) was observed in patients in comparison with age-matched persons without neuropathology. These data suggest that complex analysis of the expression of Aβ42, τ-protein, p16, and p53 in the buccal epithelium is a promising method for in vivo diagnosis of Alzheimer’s disease and assessment of the rate of aging during the development of this pathology.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04416-0
Contribution of Fetal Programming in the Formation of Cognitive
Impairments Induced by Lead Poisoning in White Rats
- Abstract: The contribution of prenatal hypoxic damage to the CNS to the formation of high sensitivity of the body to lead acetate was studied. Prenatal fetal hypoxia was modeled by the administration of sodium nitrite in doses of 5, 25, and 50 mg/kg to pregnant female rats. Cognitive capacities of mature offspring were evaluated in the radial maze test and Morris water maze test. After attaining learning criterion in the radial maze, lead acetate in a dose of 80 mg/kg was added to the drinking water of all animals over 2 weeks. Testing was performed during the exposure to the agent until disruption of the conditioned behavior. It was found that severe prenatal hypoxia (induced by the administration of 50 mg/kg sodium nitrite) impaired spatial memory, increased latency of funding the platform in Morris water maze test, and serves as a factor contributing to earlier manifestations of the neurotoxic effects of lead acetate.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04404-4
Changes in the Metastatic Properties of MDA-MB-231 Cells after IGFBP6 Gene
Knockdown Is Associated with Increased Expression of miRNA Genes
Controlling INSR , IGF1R , and CCND1 Genes
- Abstract: Metastatic cascade is associated with the process of epithelial-mesenchymal transition accompanied by changes in cell proliferation, migration, adhesion, and invasiveness mediated by the insulin-like growth factor (IGF) signal pathway. IGFBP6 protein binds IGF and prevents its interaction with receptors. IGFBP6 gene knockdown through RNA-interference inhibits cell migration and increased the rate of proliferation of breast cancer MDA-MB-231 cells. IGFBP6 knockdown cells are characterized by increased expression of MIR100 and MIRLET7A2 genes encoding hsa-miR-100-3p, hsa-miR-100-5p, hsa-let-7a-5p, and hsa-let-7a-2-3p miRNA. The target genes of these microRNAs are IGF2, IGF1R, INSR, and CCND1 associated with IGF signaling pathway and proliferative and migratory activity during the metastatic cascade. A significant decrease in the expression of INSR and CCND1 genes was demonstrated by PCR and microarray analysis.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04409-z
Amino Acid Substitutions N123D and N149D in Hemagglutinin Molecule Enhance
Immunigenicity of Live Attenuated Influenza H7N9 Vaccine Strain in
Experiment
- Abstract: We compared three cold-adapted live attenuated influenza vaccine strains prepared by reverse genetics methods on the basis of master donor virus A/Leningrad/134/17/57 and influenza H7N9 strains A/Anhui/1/2013 and A/Shanghai/1/2013. Two strains based on A/Anhui/1/2013 differed by amino acid positions 123 and 149 in HA1 (123N/149N; 123D/149D). All strains efficiently replicated in developing chicken embryos; A/Shanghai/1/2013-based strain and A/Anhui/1/2013-123N/149N variant were characterized by reduced replication in MDCK cells. Strains based on A/Anhui/1/2013 virus agglutinated erythrocytes with α2,3- and α2,6-linked sialic acid residues, whereas strain A/Shanghai/1/2013 only α2,3. In experiments with BALB/c mice, Anhui-123D/149D strain was most immunogenic and induced high crossreactive humoral immune response, therefore it can be recommended as the model virus for the construction of recombinant vector vaccines based on live attenuated influenza vaccine.
  PubDate: 2019-03-22
  DOI: 10.1007/s10517-019-04407-1
Correction to: Role of β Cell Precursors in the Regeneration of
Insulin-Producing Pancreatic β Cells under the Influence of the Pegylated
Form of Glucagon-Like Peptide 1
- Abstract: The title of the article should read: “Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of the Pegylated Form of Glucagon-Like Peptide 1”.
  PubDate: 2019-02-01
  DOI: 10.1007/s10517-019-04397-0