Subjects -> MEDICAL SCIENCES (Total: 8679 journals)
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DERMATOLOGY AND VENEREOLOGY (163 journals)                     

Showing 1 - 163 of 163 Journals sorted alphabetically
Acta Dermato-Venereologica     Open Access   (Followers: 12)
Acta Dermatovenerologica Croatica     Open Access   (Followers: 1)
Actas Dermo-Sifiliograficas     Full-text available via subscription   (Followers: 3)
Actas Dermo-Sifiliográficas (English Edition)     Full-text available via subscription   (Followers: 2)
Advances in Dermatology     Full-text available via subscription   (Followers: 15)
Advances in Skin & Wound Care     Hybrid Journal   (Followers: 30)
African Journal of AIDS Research     Hybrid Journal   (Followers: 8)
AIDS     Hybrid Journal   (Followers: 24)
AIDS Care: Psychological and Socio-medical Aspects of AIDS/HIV     Hybrid Journal   (Followers: 10)
AIDS Patient Care and STDs     Hybrid Journal   (Followers: 3)
AIDS Research and Human Retroviruses     Hybrid Journal   (Followers: 9)
AIDS Research and Therapy     Open Access   (Followers: 15)
AIDS Research and Treatment     Open Access   (Followers: 2)
Aktuelle Dermatologie     Hybrid Journal   (Followers: 7)
Allergo Journal     Full-text available via subscription   (Followers: 2)
American Journal of Clinical Dermatology     Full-text available via subscription   (Followers: 26)
American Journal of Dermatopathology     Hybrid Journal   (Followers: 18)
Anais Brasileiros de Dermatologia     Open Access   (Followers: 2)
Anaplastology     Open Access  
Annales de Dermatologie et de Vénéréologie     Full-text available via subscription  
Archives de Pédiatrie     Full-text available via subscription  
Archives de sciences sociales des religions     Open Access   (Followers: 1)
Archives des Maladies du Coeur et des Vaisseaux - Pratique     Hybrid Journal  
Archives of Dermatological Research     Hybrid Journal   (Followers: 7)
Archives of Gerontology and Geriatrics     Hybrid Journal   (Followers: 13)
Archives of Industrial Hygiene and Toxicology     Open Access   (Followers: 8)
Archives of Medical Research     Hybrid Journal   (Followers: 3)
Archives of Physical Medicine and Rehabilitation     Hybrid Journal   (Followers: 58)
Archivio di Ortopedia e Reumatologia     Hybrid Journal  
Asian Journal of Dermatology     Open Access   (Followers: 2)
ästhetische dermatologie & kosmetologie     Full-text available via subscription  
Australasian Journal of Dermatology     Hybrid Journal   (Followers: 8)
Berkala Ilmu Kesehatan Kulit dan Kelamin / Periodical of Dermatology and Venereology     Open Access  
Biomedical Dermatology     Open Access  
BMC Dermatology     Open Access   (Followers: 13)
BMJ Sexual & Reproductive Health     Hybrid Journal   (Followers: 2)
British Journal of Dermatology     Hybrid Journal   (Followers: 54)
Case Reports in Dermatological Medicine     Open Access   (Followers: 2)
Case Reports in Dermatology     Open Access   (Followers: 9)
Clinical and Experimental Dermatology     Hybrid Journal   (Followers: 13)
Clinical Dermatology Review     Open Access   (Followers: 5)
Clinical Skin Cancer     Full-text available via subscription  
Clinical, Cosmetic and Investigational Dermatology     Open Access   (Followers: 10)
Clinics in Dermatology     Hybrid Journal   (Followers: 14)
Contact Dermatitis     Hybrid Journal   (Followers: 8)
Cosmetics     Open Access   (Followers: 5)
Current Dermatology Reports     Hybrid Journal   (Followers: 7)
Current Fungal Infection Reports     Hybrid Journal   (Followers: 5)
Current HIV Research     Hybrid Journal   (Followers: 7)
Current HIV/AIDS Reports     Hybrid Journal   (Followers: 6)
Current Sexual Health Reports     Hybrid Journal   (Followers: 3)
Cutaneous and Ocular Toxicology     Hybrid Journal   (Followers: 10)
Der Hautarzt     Hybrid Journal   (Followers: 2)
Dermatitis     Hybrid Journal   (Followers: 1)
Dermato-Endocrinology     Open Access   (Followers: 2)
Dermatología Venezolana     Open Access  
Dermatologic Clinics     Full-text available via subscription   (Followers: 4)
Dermatologic Reviews     Hybrid Journal  
Dermatologic Surgery     Hybrid Journal   (Followers: 9)
Dermatologic Therapy     Hybrid Journal   (Followers: 2)
Dermatologica Sinica     Open Access  
Dermatological Nursing     Full-text available via subscription   (Followers: 2)
Dermatology     Full-text available via subscription   (Followers: 20)
Dermatology and Cosmetic     Open Access   (Followers: 8)
Dermatology and Therapy     Open Access   (Followers: 4)
Dermatology Online Journal     Open Access   (Followers: 1)
Dermatology Reports     Open Access   (Followers: 3)
Dermatology Research and Practice     Open Access   (Followers: 4)
Dermatopathology     Open Access   (Followers: 3)
Egyptian Journal of Dermatology and Venerology     Open Access   (Followers: 1)
EMC - Cosmetologia Medica e Medicina degli Inestetismi Cutanei     Full-text available via subscription  
EMC - Dermatología     Full-text available via subscription   (Followers: 1)
European Journal of Dermatology     Hybrid Journal   (Followers: 15)
Experimental Dermatology     Hybrid Journal   (Followers: 10)
Expert Review of Dermatology     Hybrid Journal   (Followers: 15)
Forum Dermatologicum     Hybrid Journal  
Graefe's Archive for Clinical and Experimental Ophthalmology     Hybrid Journal   (Followers: 9)
Güncel Dermatoloji Dergisi     Open Access  
HautinForm     Full-text available via subscription  
hautnah     Hybrid Journal  
hautnah dermatologie     Hybrid Journal  
HIV & AIDS Review     Full-text available via subscription   (Followers: 13)
HIV Clinical Trials     Hybrid Journal   (Followers: 5)
HIV Medicine     Hybrid Journal   (Followers: 3)
Indian Dermatology Online Journal     Open Access   (Followers: 3)
Indian Journal of Dermatology     Open Access   (Followers: 2)
Indian Journal of Dermatology, Venereology and Leprology     Open Access   (Followers: 4)
Indian Journal of Dermatopathology and Diagnostic Dermatology     Open Access  
Indian Journal of Drugs in Dermatology     Open Access   (Followers: 1)
Indian Journal of Paediatric Dermatology     Open Access   (Followers: 2)
Indian Journal of Sexually Transmitted Diseases and AIDS     Open Access   (Followers: 2)
International Archives of Medicine     Open Access   (Followers: 3)
International Journal of Dermatology     Hybrid Journal   (Followers: 14)
International Journal of Dermatology and Clinical Research     Open Access   (Followers: 2)
International Journal of Research in Dermatology     Open Access   (Followers: 1)
International Journal of STD & AIDS     Hybrid Journal   (Followers: 7)
International Journal of Women's Dermatology     Open Access   (Followers: 1)
International STD Research & Reviews     Open Access   (Followers: 1)
JAAD Case Reports     Open Access   (Followers: 1)
JAIDS : Journal of Acquired Immune Deficiency Syndromes     Hybrid Journal   (Followers: 4)
JAMA Dermatology     Full-text available via subscription   (Followers: 49)
JAMA Facial Plastic Surgery     Full-text available via subscription   (Followers: 13)
JMIR Dermatology     Open Access   (Followers: 1)
Journal of AIDS & Clinical Research     Open Access   (Followers: 3)
Journal of Clinical & Experimental Dermatology Research     Open Access   (Followers: 6)
Journal of Clinical and Investigative Dermatology     Open Access   (Followers: 2)
Journal of Cosmetic Dermatology     Hybrid Journal   (Followers: 11)
Journal of Cosmetics, Dermatological Sciences and Applications     Open Access   (Followers: 7)
Journal of Cutaneous Immunology and Allergy     Open Access  
Journal of Cutaneous Medicine and Surgery     Full-text available via subscription  
Journal of Dermatological Research     Open Access  
Journal of Dermatological Science     Hybrid Journal   (Followers: 2)
Journal of Dermatological Science Supplement     Full-text available via subscription   (Followers: 1)
Journal of Dermatological Treatment     Hybrid Journal   (Followers: 2)
Journal of Dermatology & Dermatologic Surgery     Open Access   (Followers: 1)
Journal of General-Procedural Dermatology & Venereology Indonesia     Open Access  
Journal of HIV/AIDS & Social Services     Hybrid Journal   (Followers: 9)
Journal of Investigative Dermatology     Hybrid Journal   (Followers: 28)
Journal of Investigative Dermatology Symposium Proceedings     Full-text available via subscription  
Journal of Sexual Medicine     Hybrid Journal   (Followers: 6)
Journal of Sexually Transmitted Diseases     Open Access   (Followers: 3)
Journal of Skin and Stem Cell     Open Access   (Followers: 3)
Journal of Skin Cancer     Open Access   (Followers: 3)
Journal of Surgical Dermatology     Open Access   (Followers: 1)
Journal of the American Academy of Dermatology     Full-text available via subscription   (Followers: 37)
Journal of the Dermatology Nurses' Association     Hybrid Journal   (Followers: 3)
Journal of the Egyptian Women’s Dermatologic Society     Partially Free  
Journal of the European Academy of Dermatology and Venereology     Hybrid Journal   (Followers: 15)
Journal of the International AIDS Society     Open Access   (Followers: 10)
Journal of the Saudi Society of Dermatology & Dermatologic Surgery     Open Access   (Followers: 1)
Karger Kompass Dermatologie     Full-text available via subscription  
Karger Kompass Pneumologie     Full-text available via subscription   (Followers: 1)
Langenbeck's Archives of Surgery     Hybrid Journal   (Followers: 4)
Medical and Surgical Dermatology     Hybrid Journal   (Followers: 1)
Medical Mycology     Open Access   (Followers: 4)
Nepal Journal of Dermatology, Venereology & Leprology     Open Access   (Followers: 1)
Neurobehavioral HIV Medicine     Open Access   (Followers: 2)
OA Dermatology     Open Access   (Followers: 1)
Open AIDS Journal     Open Access  
Open Dermatology Journal     Open Access  
Perspectives On Sexual and Reproductive Health     Hybrid Journal   (Followers: 7)
Pigment International     Open Access   (Followers: 1)
Psoriasis : Targets and Therapy     Open Access   (Followers: 4)
Revista Internacional de Ciencias Podológicas     Open Access  
SAHARA : Journal of Social Aspects of HIV / AIDS Research Alliance     Open Access   (Followers: 5)
Scars, Burns & Healing     Open Access  
Serbian Journal of Dermatology and Venereology     Open Access   (Followers: 1)
Sex Education: Sexuality, Society and Learning     Hybrid Journal   (Followers: 5)
Sexual & Reproductive Healthcare     Hybrid Journal   (Followers: 2)
Sexual Health     Hybrid Journal   (Followers: 4)
Sexually Transmitted Diseases     Hybrid Journal   (Followers: 7)
Sexually Transmitted Infections     Hybrid Journal   (Followers: 6)
Skin Appendage Disorders     Full-text available via subscription   (Followers: 1)
Skin Pharmacology and Physiology     Full-text available via subscription   (Followers: 7)
Skin Research and Technology     Hybrid Journal   (Followers: 6)
Southern African Journal of HIV Medicine     Open Access   (Followers: 3)
Sri Lanka Journal of Sexual Health and HIV Medicine     Open Access  
Studies in Gender and Sexuality     Hybrid Journal   (Followers: 21)
Surgical & Cosmetic Dermatology     Open Access   (Followers: 3)
The Journal of Dermatology     Hybrid Journal   (Followers: 4)
The Rose Sheet     Full-text available via subscription   (Followers: 2)
Vestnik dermatologii i venerologii     Open Access  
Veterinary Dermatology     Hybrid Journal   (Followers: 8)


Similar Journals
Journal Cover
Journal of Dermatological Science Supplement
Number of Followers: 1  
  Full-text available via subscription Subscription journal
ISSN (Print) 1574-0757
Published by Elsevier Homepage  [3200 journals]
  • Reconstructed human skin: From photodamage to sunscreen photoprotection
           and anti-aging molecules
    • Authors: Corinne Vioux-Chagnoleau; François Lejeune; Juliette Sok; Cécile Pierrard; Claire Marionnet; Françoise Bernerd
      Abstract: Publication date: December 2006
      Source:Journal of Dermatological Science Supplement, Volume 2, Issue 1
      Author(s): Corinne Vioux-Chagnoleau, François Lejeune, Juliette Sok, Cécile Pierrard, Claire Marionnet, Françoise Bernerd
      Background Acute or repetitive sun exposure has been shown to be responsible for various deletorious consequences such as sunburn reaction, photoaging and photocancers. Determination of early biological events occurring after UV exposure is essential for photoprotection. Objectives Using a human reconstructed skin in vitro, comprising both a fully differentiated epidermis and a living dermal equivalent, the effects of UVB and UVA were analysed in keratinocytes and fibroblasts. The efficiency of sunscreen protection was afforded after topical application. The effect of Vitamin C was analyzed. Methods Reconstructed skin was exposed to UVB, UVA or SSR. Morphological aspect, DNA lesions, sunburn cells, MMP-1 were evaluated. Sunscreen photoprotection was assessed with formulations having different profile of absorption or photostability. Vitamin C was added to the culture medium during reconstruction of the skin. Results While UVB essentially induced direct epidermal damage such as DNA lesions or sunburn cells, UVA reached the dermis and led to alterations in fibroblasts and dermal extracellular matrix. Both UVB and UVA, alone or combined using solar simulated radiation (SSR), induced MMP-1 production, directly in dermal fibroblasts after UVA exposure, or through release of soluble epidermal factors after UVB. Based on the identification of these biological end-points, evaluation of sunscreeen photoprotection was performed after topical application of formulations. The data showed that broad UVB+UVA spectrum profile together with photostability are required for a real efficient protection. Finally, we found that Vitamin C could improve the morphogenesis of dermal epidermal junction, through its ability to promote synthesis of extracellular matrix and basement membrane proteins. Conclusions These results may help in understanding the beneficial effects of such molecule in the treatment of photoaged skin. Altogether, these data emphazied the fact that human skin reconstructed in vitro is a valuable tool for research studies as well as for evaluation of compounds designed to prevent or correct photodamage.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2006.08.001
      Issue No: Vol. 2, No. 1 (2017)
  • Thermal aging: A new concept of skin aging
    • Authors: Jin Young Seo; Jin Ho Chung
      Abstract: Publication date: December 2006
      Source:Journal of Dermatological Science Supplement, Volume 2, Issue 1
      Author(s): Jin Young Seo, Jin Ho Chung
      Background Sunlight damages human skin, resulting in a wrinkled appearance. Human skin temperature, measured inside the dermis by a needle-type thermometer, can be increased up to about 40°C in direct summer midday sunlight within 15–20min, and this heat may contribute significantly to sun-induced skin damage. Recent studies suggest that heat as well as UV may play an important role in premature skin aging. However, our knowledge about the effects of heat or infrared light, which certainly increase the temperature of the skin and may possibly interfere with or enhance the damaging effects of UV, on the development of skin aging is limited. Objectives This review provides an outline of the thermal effects on skin aging process in human skin.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2006.08.002
      Issue No: Vol. 2, No. 1 (2017)
  • Biological responses to extremely low-frequency electromagnetic fields
    • Authors: Junji Miyakoshi
      Abstract: Publication date: December 2006
      Source:Journal of Dermatological Science Supplement, Volume 2, Issue 1
      Author(s): Junji Miyakoshi
      Background During the past decade, the biological effects of extremely low-frequency (ELF) electromagnetic fields have been investigated in many countries. Also, many papers for the responses to ELF electromagnetic fields had been published in both in vitro and in vivo. Objective I review the cellular and molecular effects and mouse skin tumorigenesis of ELF electromagnetic fields. Methods Cellular genotoxicity including chromosomal aberrations, DNA stand breaks, and mutation and the skin tumorigenesis in mice were examined using the conventional experimental methods. Results It is considered that sole exposure to ELF electromagnetic fields at an intensity of less than several hundred microtesla (μT) may not affect cell growth, cellular genotoxicity (such as DNA strand breaks, chromosome aberrations, mutations), gene expression, and signal transduction. However, exposure to ELF electromagnetic fields at an extremely high intensity, i.e., 400mT, can induce chromatid-type aberrations, mutations, and induce expression of specific genes (NOR-1). Exposure to ELF electromagnetic fields at relatively high intensity (>several mT) may also potentiate the cellular damage induced by external factors, such as ionizing radiation and several chemical agents. In a recent animal study, it was reported that exposure to 50Hz magnetic fields enhanced the rate of UV-induced tumor development in mouse skin Conclusion It is unlikely that exposure to extremely low-density ELF electromagnetic fields at microtesla levels would evoke large changes in cells. However, many unclear issues remain to be resolved. The possible mechanisms of action of ELF electromagnetic fields are discussed.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2006.08.003
      Issue No: Vol. 2, No. 1 (2017)
  • A method for rapid evaluation of photoaging by measuring fluorescence
           intensity of green fluorescent protein due to elastin promoter activity
    • Authors: Naoko Kondo; Hitoshi Takeda; Takahide Kaneko; Takayuki Aizu; Ryuta Moritsugu; Atsushi Kon; Nakano Hajime; Katsumi Hanada
      Abstract: Publication date: December 2006
      Source:Journal of Dermatological Science Supplement, Volume 2, Issue 1
      Author(s): Naoko Kondo, Hitoshi Takeda, Takahide Kaneko, Takayuki Aizu, Ryuta Moritsugu, Atsushi Kon, Nakano Hajime, Katsumi Hanada
      Background Photoaging increases the deep furrows of the facial skin. This can be seen histologically as solar elastosis in the upper layer of the dermis. In sunlight, ultraviolet B (UVB) light plays a pivotal role in forming the wrinkles, because it enhances elastin gene expression and matrix metalloproteases (MMPs) at the transcriptional level in dermal fibroblasts. Sunscreens are believed to inhibit UVB-induced wrinkle formation in humans, but few methods have accurately assessed the protective effects of sunscreens in vivo to date. A major reason is that it takes a long time to cause the deep facial lines. Objective To develop a rapid and simple in vivo evaluation system, to assess the inhibitory effects of sunscreens against photoaging, a new experimental method was studied. Methods The promoter region of the human elastin gene was cloned into a green fluorescent protein (GFP) vector. The plasmid DNA was transfected into 3T3 cells and stable transfectants (3T3/Eln-GFP) were established. These cells were injected into the dorsal skin of nude mice. After UVB irradiation, the GFP fluorescence intensity was measured by fluorescence emission spectroscopy (FEMS) on the dorsal skin of the mouse. Results UVB irradiation of 3T3/Eln-GFP cells increased both the fluorescence intensity and the expression of GFP. Increased GFP fluorescence intensity could be measured from the body surface with FEMS at the injected site of the cells after UVB irradiation in vivo. Conclusion A rapid and simple evaluation system for human elastin promoter activity by UVB was established, and we propose it to be an useful method for objective assessment of sunscreens designed to prevent wrinkle formation.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2006.08.004
      Issue No: Vol. 2, No. 1 (2017)
  • Efficacy of thermal stimulation on wrinkle removal via the enhancement of
           collagen synthesis
    • Authors: Yasushi Yamamoto; Kei Obayashi; Yuri Okano; Yasuhiro Satoh; Hitoshi Masaki; Yoko Funasaka
      Abstract: Publication date: December 2006
      Source:Journal of Dermatological Science Supplement, Volume 2, Issue 1
      Author(s): Yasushi Yamamoto, Kei Obayashi, Yuri Okano, Yasuhiro Satoh, Hitoshi Masaki, Yoko Funasaka
      Background Heat shock protein 47 (HSP47) is a specific chaperone of procollagen. It is important to elucidate the effects of heat on collagen synthesis both in vitro and in vivo. Objectives We examined the effects of heat on collagen synthesis and the role of HSP47 using an in vitro system, and we also characterized the efficacy of wrinkle removal by heat treatment of human skin. Methods Normal human fibroblasts were used to evaluate the relationship between heat-induced collagen synthesis and HSP47 using an enzyme-linked immunosorbent assay (ELISA), reverse transcription-polymerase chain reaction (RT-PCR) and antisense. Heat stimulation of 6-week-old hairless mice (HOS:HR-1) was performed at varying temperatures (38, 40 and 42°C) 3 days a week for 4 weeks, then the amount of collagen was determined by hydroxyproline content. For clinical evaluation, the left side of the face of 31 women (aged 36–55 years), was treated with heat 10min a day for 2 months using hot steam which kept the skin surface temperature at 40–42°C. Evaluations were performed using a visual analog scale, by replica taking, and with a Cutometer, prior to and 4 and 8 weeks after the heat treatment. Results The in vitro study showed that heat treatment enhanced collagen biosynthesis by up-regulating HSP47 mRNA and protein expression but not procollagenα1(I). Antisense inhibition of HSP47 prevented the increase of collagen synthesis induced by heat. Heat treatment at 40–42°C enhanced hydroxyproline content and improved wrinkles/sags of the facial skin. Conclusions These findings indicate that heat treatment at 40–42°C has a beneficial therapeutic potential to repair wrinkles and sags in the skin through the up-regulation of collagen synthesis.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2006.08.006
      Issue No: Vol. 2, No. 1 (2017)
  • Molecular alterations of decorin in photoaging process
    • Authors: Yoshihito Kawashima; Nobuaki Ohto; Akinori Kiso; Toshimitsu Kambara; Shigeru Sugano; Hiroo Sawada; Akimichi Morita
      Abstract: Publication date: December 2006
      Source:Journal of Dermatological Science Supplement, Volume 2, Issue 1
      Author(s): Yoshihito Kawashima, Nobuaki Ohto, Akinori Kiso, Toshimitsu Kambara, Shigeru Sugano, Hiroo Sawada, Akimichi Morita
      Background Decorin, a small leucine-rich proteoglycan, plays important biological roles, such as cell adhesion, migration, and proliferation in various matrix assemblies. The most important roles of decorin are to interact with several types of collagen and to modulate collagen fibrillogenesis in the body. Objective To analyze decorin expression in photoaging skin and the underlying molecular mechanisms of ultraviolet A (UVA) radiation in decorin production. Method Decorin and type I collagen production were immunohistochemically determined in photoaged rat skin. UVA-induced decorin production in the cultured fibroblasts was determined by western blot analysis. Results Decorin and aberrant type I collagen levels were increased in the upper dermis of rat skin treated with two minimum erythema dose (MED) of UV irradiation 120 times. Decorin production from the cultured fibroblasts was drastically increased by UVA irradiation and this was inhibited by sodium azide. Decorin production from the cultured fibroblast was also increased by 2–200pg/mL of IL-1α and UVA-induced decorin was inhibited by anti-IL-1α antibody. Conclusion These results suggest that the increase of decorin production from fibroblasts after UVA irradiation is induced by IL-1α, which is released through the generation of singlet oxygen induced by UVA, and that the altered expression of decorin triggers the production of aberrant collagen, which might accelerate photoaging.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2006.08.007
      Issue No: Vol. 2, No. 1 (2017)
  • Enhancement of the photodynamic effects on human oral squamous cell
           carcinoma cell lines by treatment with calcipotriol
    • Authors: Yoichi Akita; Ken-ichi Kozaki; Tomohiro Takeo; Motonobu Ohmura; Atsuko Nakagawa; Takeshi Yanagishita; Yoshiaki Kazaoka; Tadashige Nozaki; Kazuhisa Yokoo; Mitsuko Shinohara; Daisuke Watanabe; Tatsushi Kawai; Shiro Yamada; Yasuhiko Tamada; Kiyoshi Ohura; Yoshinari Matsumoto
      Abstract: Publication date: December 2006
      Source:Journal of Dermatological Science Supplement, Volume 2, Issue 1
      Author(s): Yoichi Akita, Ken-ichi Kozaki, Tomohiro Takeo, Motonobu Ohmura, Atsuko Nakagawa, Takeshi Yanagishita, Yoshiaki Kazaoka, Tadashige Nozaki, Kazuhisa Yokoo, Mitsuko Shinohara, Daisuke Watanabe, Tatsushi Kawai, Shiro Yamada, Yasuhiko Tamada, Kiyoshi Ohura, Yoshinari Matsumoto
      Background 5-Aminolaevulinic acid (ALA)-based photodynamic therapy (PDT) for patients with skin and oral diseases is a highly sophisticated procedure, but the incidence of disease recurrence after treatment with ALA-based PDT is somewhat alarming. Calcipotriol, an analogue of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), has been reported to regulate the proliferation and differentiation of keratinocytes. Objective In order to obtain even greater efficacy of ALA-based PDT, we investigated the synergistic effects of calcipotriol as an adjunct to ALA-based PDT for human oral squamous cell carcinoma (SCC) cell lines. Methods Intracellular protoporphyrin IX (PpIX) converted from exogenous ALA in SCC cell lines treated with/without calcipotriol was measured by a fluorescencemeter. Then, the in vitro effects of calcipotriol, the cyclooxygenase (COX)-2 selective inhibitor (nimesulide), ALA-based PDT and their combination on two SCC cell lines, HSC-2 (a COX-2 high expresser) and HSC-4 (a COX-2 non-expresser), were determined by MTT assay and double-staining for annexin V and propidium iodide. Results The concentration of intracellular PpIX was increased in four of the eight SCC cell lines (50%) treated with calcipotriol. The greatest alteration of intracellular PpIX was found in HSC-4 (1.9-fold). The combination of calcipotriol and ALA-based PDT remarkably inhibited cellular proliferation and induced cellular death of both HSC-2 and HSC-4. Whereas, this morphological damage was more serious in HSC-4 than in HSC-2. Furthermore, these effects were almost equivalent to the synergistic effect of the combination of nimesulide and ALA-based PDT on HSC-2. Conclusions The present study suggests that treatment with calcipotriol enhances the photodynamic effects on SCC via the accumulation of exogenous ALA-dependent PpIX.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2006.08.005
      Issue No: Vol. 2, No. 1 (2017)
  • Improvement of wrinkles by an all-trans-retinoic acid derivative,
           d-δ-tocopheryl retinoate
    • Authors: Yuri Okano; Kei Obayashi; Syoichi Yahagi; Kouji Kurihara; Satoko Kaburagi; Yoshiko Kurata; Hitoshi Masaki
      Abstract: Publication date: December 2006
      Source:Journal of Dermatological Science Supplement, Volume 2, Issue 1
      Author(s): Yuri Okano, Kei Obayashi, Syoichi Yahagi, Kouji Kurihara, Satoko Kaburagi, Yoshiko Kurata, Hitoshi Masaki
      Background All-trans-retinoic acid (RA) is well known as a potent anti-aging drug. However, RA is generally difficult to use topically due to its irritancy and mutagenicity. Therefore, we synthesized a new RA derivative, d-δ-tocopheryl retinoate (TR). Objective To evaluate the potency of TR on anti-aging effects, investigating responses of human skin cells, keratinocytes and fibroblasts. Method To examine the potential for TR to remove reactive oxygen species (ROS), we used an electron spin resonance (ESR) spin-trapping method and a chemical assay. To investigate the effects of TR on skin cells, we used HaCaT cells, human keratinocytes, and human fibroblasts. Further, clinical test was carried out. Results In ESR study, TR showed the quenching abilities against singlet oxygen. In a biological system, TR significantly suppressed the elongation of DNA comet tails in HaCaT keratinocytes after UVB irradiation. The sum of those results suggests that TR has a capability to remove oxidative stress. TR had no effect on matrix metalloprotainase-1 (MMP-1) expression by UVA-irradiated fibroblasts. TR increased collagen production by fibroblasts and hyaluronic acid (HA) production by keratinocytes. We conducted a clinical study on wrinkle improvement using eight human volunteers. Treatment with TR exhibited a significant reduction in the depth of linear wrinkles, showing the potency of TR to improve wrinkles. Conclusion Anti-wrinkle efficacy of TR is suggested to involve removing oxidative stress and modulating the metabolism of the extracellular matrix.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2006.08.008
      Issue No: Vol. 2, No. 1 (2017)
  • Different apoptotic patterns observed in tissues damaged by phenol and TCA
    • Authors: Yuki Yamamoto; Koji Uede; Toshio Ohtani; Akiko Kishioka; Toshiko Tanaka; Fukumi Furukawa
      Abstract: Publication date: December 2006
      Source:Journal of Dermatological Science Supplement, Volume 2, Issue 1
      Author(s): Yuki Yamamoto, Koji Uede, Toshio Ohtani, Akiko Kishioka, Toshiko Tanaka, Fukumi Furukawa
      Herein we intended to understand the histological differences in tissues treated with phenol and trichloroacetic acid (TCA) peels by study the expression of proteins involved in apoptotic cell death using immunohistochemical methods. Phenol penetrated into skin quickly and induced histological changes in endothelial cells in the dermis, which were detected by the TUNEL method and the expression of activated Caspase-3 molecules. However, we did not observe up-regulation of p53 and Fas antigens. Meanwhile degenerations from TCA peels were detected by the TUNEL method, but not observed with Caspase-3 activation. These findings suggest that skin degenerations from phenol peels undergo Caspase-3-mediated apoptosis from that of TCA peels in pathogenesis.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2006.08.009
      Issue No: Vol. 2, No. 1 (2017)
  • Ultraviolet (UV) light irradiation induced signal transduction in skin
    • Authors: Yiru Xu; Gary J. Fisher
      Abstract: Publication date: December 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 2
      Author(s): Yiru Xu, Gary J. Fisher
      The purpose of this article is to offer perspective on current understanding of ultraviolet (UV) irradiation-induced signal transduction, with emphasis on membrane-initiated, non-nuclear signaling events. We will describe the role of UV-induced signaling in the mechanism of premature skin aging (photoaging). UV irradiation serves as a universal ligand to active cell surface receptors and to induce assembly of signaling complexes at the plasma membrane. These complexes activate cytosolic protein kinase cascades that relay signals to the cell nucleus, thereby regulating the activity of a variety transcription factors. Key cellular responses are up-regulation of matrix-degrading metalloproteinases, and down-regulation of extracellular matrix biosynthesis. The resultant net deficit of structural integrity of skin connective tissue is one of the major factors that contributes to the phenotype of photoaging. Insights into the molecular mechanisms of UV-induced signal transduction will eventually translate into new, effective methods to prevent UV-induced skin damage.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2005.06.002
      Issue No: Vol. 1, No. 2 (2017)
  • DNA repair initiated by glycosylases in the nucleus and mitochondria of
    • Authors: Masashi Takao; Akira Yasui
      Abstract: Publication date: December 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 2
      Author(s): Masashi Takao, Akira Yasui
      Oxidative DNA damage causes blocks in transcription and replication and introduces errors leading to cell death and genomic instability. Extensive molecular analysis of repair mechanisms for oxidative DNA damage has revealed the strategy of mammalian cells against the threat posed by reactive oxygen species to the genetic information. Most oxidative base damage is first recognized by various glycosylases, which remove the base and initiate base excision repair, a repair mechanism widely distributed in life. However, disruptions of glycosylase genes have shown either no effect or rather mild phenotypes in mice, suggesting that the frontline defence against oxidative DNA damage has back-up systems. Indeed, a number of novel DNA glycosylases with overlapping substrate specificity were recently identified in mammalian cells. Several glycosylases are transported into the mitochondria too, indicating the importance of glycosylases and base damage repair in the mitochondria genome. Thus, mammalian cells survive the flood of oxidative DNA damage by means of extensive repair of the damage. Besides repair of DNA damage, mechanisms for tolerating DNA damage at replication have recently been discovered. Cells may tolerate residual damage at replication but thereby risk generating mutations.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2005.06.001
      Issue No: Vol. 1, No. 2 (2017)
  • Decrease of antioxidants and the formation of oxidized diacylglycerol in
           mouse skin caused by UV irradiation
    • Authors: Yutaka Tanino; Arief Budiyanto; Masato Ueda; Akihiro Nakada; Wei Tak Nyou; Makoto Yanagisawa; Masamitsu Ichihashi; Yorihiro Yamamoto
      Abstract: Publication date: December 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 2
      Author(s): Yutaka Tanino, Arief Budiyanto, Masato Ueda, Akihiro Nakada, Wei Tak Nyou, Makoto Yanagisawa, Masamitsu Ichihashi, Yorihiro Yamamoto
      UV irradiation induces inflammation and immunosuppression, and may cause skin cancer. To elucidate the molecular mechanism of these pathological events we exposed hairless mice to UVA at a dose of 5J/cm2. It resulted in the decay of ascorbate, urate, and reduced form of coenzyme Q, and the formation of oxidized diacylglycerols which can activate protein kinase C and human neutrophils. UVB irradiation also caused the decay of ascorbate and urate, and the formation of oxidized diacylglycerols. These results show that the irradiation of both UVA and UVB induces free radical oxidation of antioxidants and lipids, and indicate that oxidized diacylglycerols may play a role as an endogenous tumor promoter in UV-induced skin carcinogenesis.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2005.06.003
      Issue No: Vol. 1, No. 2 (2017)
  • Tissue-specific downregulation of type VII collagen gene (COL7A1)
           transcription in cultured epidermal keratinocytes by ultraviolet A
           radiation (UVA) and UVA-inducible cytokines, with special reference to
           cutaneous photoaging
    • Authors: Atsushi Kon; Hitoshi Takeda; Noriko Ito; Katsumi Hanada; Keiichi Takagaki
      Abstract: Publication date: December 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 2
      Author(s): Atsushi Kon, Hitoshi Takeda, Noriko Ito, Katsumi Hanada, Keiichi Takagaki
      Background Type VII collagen is the major component of anchoring fibrils, which stabilize the attachment of the basement membrane zone to the dermis. Expression of type VII collagen in epidermal keratinocytes and formation of anchoring fibrils in the basement membrane zone are reduced in photoaged skin, suggesting their involvement in the pathophysiology of photoaging. Objective To investigate the effects of ultraviolet A radiation (UVA) and UVA-inducible cytokines, tumor necrosis factor (TNF)-α and interleukin (IL)-1β on type VII collagen gene (COL7A1) transcription in epidermal keratinocytes. Methods Cultured epidermal and HaCaT keratinocytes were transiently/stably transfected with plasmid constructs containing sequential 5′-end deletions of the COL7A1 promoter, linked to luciferase or the GFP gene. Twenty-four hours after treatment with either UVA, TNF-α or IL-1β, luciferase activity and GFP expression of TNF-α and IL-1β were detected by luminometer or fluorescence microscopy, respectively. Results UVA, TNF-α and IL-1β all decreased COL7A1 promoter activity in cultured epidermal keratinocytes as well as GFP expression in HaCaT keratinocytes. Deletion analysis revealed that the UVA- and cytokine-responsive region of COL7A1 lies between nucleotides −524 and −22. Conclusion UVA, TNF-α and IL-1β inhibit COL7A1 expression at the transcriptional level by acting on nucleotides −524 to −22 of the promoter region. These results suggest that UVA-induced downregulation of COL7A1 transcription in epidermal keratinocytes diminishes anchoring fibrils formation, resulting in skin fragility. Additional external factors including physical forces and UVB radiation in the sun-exposed areas may further promote deep wrinkle formation.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2005.06.004
      Issue No: Vol. 1, No. 2 (2017)
  • Cytokine profiles of skin lesions in murine lupus models
    • Authors: Takeshi Nishide; Takashi Yoshimasu; Takaharu Ikeda; Naohiro Seo; Toshio Ohtani; Fukumi Furukawa
      Abstract: Publication date: December 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 2
      Author(s): Takeshi Nishide, Takashi Yoshimasu, Takaharu Ikeda, Naohiro Seo, Toshio Ohtani, Fukumi Furukawa
      Background Skin lesions of cutaneous lupus erythematosus (LE) have a number of infiltrating T cells, and these T cells and their related cytokines are involved in the development of skin lesions. Objective In order to verify the roles of T cells and their related cytokines, we compared the cytokine profiles of LE-like skin lesions in experimentally-induced knock out (KO) mice and autoimmune-prone mice. Methods Lesional skin samples were obtained from CD4 KO mice and TCRα KO mice of the B6 background treated with ultraviolet B light (UVBL) and fluorouracil (FU), and MRL/lpr mice. The levels of mRNAs for TNF-α, IFN-γ, IL-2, IL-4, IL-10 and IL-12 were determined by RT-PCR in these skin samples. Results Combination treatment with UVBL and FU to TCRα KO mice and CD4 KO mice revealed the importance of CD4+ CD8− γδT cells in the induction of drug induced cutaneous LE. Moreover, IL-12, IFN-γ and TNF-α were upregulated in TCRα KO mice treated with UVBL and FU. In contrast, IFN-γ was not elevated in the spontaneous LE-like skin lesions of MRL/lpr mice. Conclusions Cytokine profile pattern varies from strain to strain, and that murine cutaneous LE models, like human LE patients, manifest a wide range of immunological abnormalities, including cytokines.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2005.06.005
      Issue No: Vol. 1, No. 2 (2017)
  • A novel anti-photoaging ingredient with the effect of iron sequestering
    • Authors: Yukiko Ishitsuka; Misako Kobayashi; Kumi Arakane; Tadashi Suzuki; Manabu Kitazawa; Takashi Nakano; Keiji Iwasaki; Kazutami Sakamoto
      Abstract: Publication date: December 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 2
      Author(s): Yukiko Ishitsuka, Misako Kobayashi, Kumi Arakane, Tadashi Suzuki, Manabu Kitazawa, Takashi Nakano, Keiji Iwasaki, Kazutami Sakamoto
      Background The reactive oxygen species generated by ultraviolet rays causes various types of cutaneous damage, such as lipid peroxidation and denaturation of the extra-cellular matrix. The accumulation of such damage contributes to skin aging, especially the formation of wrinkles. It has been suggested that iron ions, released from iron-binding proteins by ultraviolet rays, promote the production of radicals and accelerate the formation of wrinkles. Thus, sequestering iron ions might be effective in suppressing wrinkle formation. Objective Our purpose is to develop new cosmetic materials with iron sequesteing capacity to suppress skin photoaging, especially wrinkle formation. Method We designed a novel compound, N-(4-pyridoxylmethylene)-l-serine (PYSer), as a cosmetic material with a structure similar to a biological iron sequestering protein. We studied its effect on suppressing hydroxyl radical generation and wrinkle formation in a mouse model for skin photoaging. Results PYSer almost completely inhibited the generation of hydroxyl radicals by iron ions. In contrast, EDTA, a typical metal chelator, did not suppress hydroxyl radical production, but enhanced it. Furthermore, PYSer delayed UVB-induced wrinkle formation and skin thickening. Conclusion This study demonstrates that PYSer suppresses photoaging, particularly, wrinkle formation and skin thickening.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2005.06.006
      Issue No: Vol. 1, No. 2 (2017)
  • Overview of Yusho
    • Authors: Masutaka Furue; Takeshi Uenotsuchi; Kazunori Urabe; Takehiko Ishikawa; Masao Kuwabara
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): Masutaka Furue, Takeshi Uenotsuchi, Kazunori Urabe, Takehiko Ishikawa, Masao Kuwabara
      Background: Yusho is a type of food poisoning from rice bran oil contaminated with polychlorinated biphenyls (PCBs) and various dioxins such as polychlorinated dibenzofurans (PCDFs). The victims of Yusho suffered from dermatological manifestations (acneform eruptions, comedones, etc.) in association with systemic, ophthalmological, and mucosal symptoms. Objective: To analyze the relationship between the concentrations of dioxins/PCBs and the subjective/objective complaints of patients with Yusho. Methods: We recently started to measure the blood levels of dioxins in the annual medical check-up of Yusho patients. In addition, we reviewed the clinical and epidemiological findings elucidated over the past 36 years by the Study Group for Yusho. Results and conclusion: High amounts of PCBs and PCDFs are still present in a number of patients with Yusho. The majority of laboratory findings, except for triglyceride concentration, were within normal limits throughout the clinical course. However, the patients still suffered from various mucocutaneous and subjective symptoms, and these symptoms were correlated to the blood levels of polychlorinated congeners. The development of therapeutic interventions is warranted in the near future.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2005.03.002
      Issue No: Vol. 1, No. 1 (2017)
  • Behavior and toxic effects of PCBs and PCDFs in Yusho patients for 35
    • Authors: Yoshito Masuda
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): Yoshito Masuda
      Background: Yusho polychlorinated biphenyl (PCB) poisoning occurred in northern Kyushu in 1968, and the patients have been suffering from various symptoms for 35 years. From the epidemiological survey of 141 Yusho patients in Fukuoka, the total amounts of PCBs, polychlorinated dibenzofurans (PCDFs) and the toxic equivalent quantity (TEQ) ingested by each patient were calculated to be 633, 3.4 and 0.62mg, respectively. Objectives: The purpose of this paper is to review the behavior and toxic effects of these polychlorinated congeners in Yusho patients. Results and conclusions: From the follow-up data of three Yu-cheng patients and five Yusho patients, fat-based concentrations of TEQ and PCBs in the Yusho patients were estimated to have decreased from 40ppb and 75ppm, respectively, in 1969, to 0.6ppb and 2.3ppm, respectively, in 1999. Estimated median half-lives of three PCDFs and six PCBs were 3.0 and 4.6 years, respectively, in the first 15 years after the incident, and 5.4 and 14.6 years, respectively, in the following 15 years. Patients have recovered gradually from the typical Yusho symptoms of acneform eruptions, dermal pigmentation and increased eye discharge. However, enzyme- and/or hormone-mediated signs of high serum triglyceride, high serum thyroxine, immunoglobulin disorder, etc. remain persistent for more than 30 years.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.jdermsci.2004.12.005
      Issue No: Vol. 1, No. 1 (2017)
  • Improvement in dioxin analysis of human blood and their concentrations in
           blood of Yusho patients
    • Authors: Takashi Todaka; Hironori Hirakawa; Tsuguhide Hori; Kazuhiro Tobiishi; Takao Iida
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): Takashi Todaka, Hironori Hirakawa, Tsuguhide Hori, Kazuhiro Tobiishi, Takao Iida
      Background and objective: Over 35 years have passed since the Yusho incident. We have determined the concentrations of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and non-ortho-coplanar polychlorinated biphenyls (Co-PCBs) in blood samples collected from Yusho patients to establish new criteria for Yusho. Considering the fact that the concentrations of PCDDs, PCDFs and Co-PCBs in the blood samples of about 300 Yusho patients living in Japan were scheduled for measurement in 2002, it was desirable to develop more effective methods to speed up the pretreatment procedure for blood samples. In this study, we improved a method that allows many blood samples to be treated in a short period with high reproducibility in comparison with the previously described method. Using our method, we measured the concentrations of PCDDs, PCDFs and Co-PCBs in blood collected from 279 Yusho patients in 2002 and 269 Yusho patients in 2003, and compared the results with those of 52 normal controls. Methods: The extraction procedure of PCDDs, PCDFs and Co-PCBs from the blood samples was simplified. Concentrations of the PCDDs, PCDFs and Co-PCBs were measured using high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS) equipped with a solvent-cut large volume injection system. Results and conclusion: The lipid content and the concentration of each isomer of PCDDs, PCDFs and Co-PCBs in blood determined using the improved method were almost equal to those obtained by dioxin analysis organizations that used the conventional method to analyze the same blood samples. The improved method demonstrated high reproducibility based on experiments conducted using the same serum samples. These findings indicate that the improved method is essentially equivalent to the conventional method. From the concentrations of PCDDs, PCDFs and Co-PCBs in blood samples of Yusho patients measured by the improved method, it became clear that even now Yusho patients still have a much higher concentration of PCDFs in their blood than do unaffected people more than 35 years after the Yusho incident.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.jdermsci.2004.12.006
      Issue No: Vol. 1, No. 1 (2017)
  • Blood chemistry, alpha-fetoprotein and hepatitis B surface antigen in
    • Authors: Hiroshi Tsuji; Yasuo Ito
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): Hiroshi Tsuji, Yasuo Ito
      Background and Objective: An incident of accidental human exposure to polychlorinated biphenyls (PCBs) occurred in the western part of Japan in 1968. The disease is known as Yusho, because its cause was the ingestion of rice bran oil that was contaminated with PCBs. The various symptoms such as acneform skin eruptions were observed in the early stage in Yusho patients. An important fact is that polychlorinated dibenzofurans (PCDFs) were detected in the contaminated rice oil. PCDFs have a much higher toxicity than do PCBs. Analysis of blood concentration of PCDFs was performed throughout Japan in 2002. There have been no reports on the relationship between blood concentration of PCDFs and blood chemistry, alpha-fetoprotein or hepatitis B surface antigen (HBsAg) in Yusho. This is the first study to report on the relationship between blood concentration of PCDFs and blood chemistry, alpha-fetoprotein or HBsAg in Yusho. Methods: We analyzed blood chemistry by measuring the following 20 items—total protein, serum albumin, alanine and aspartate aminotransferase, lactate dehydrogenase, alkaline phosphatase, leucine aminopeptidase, gamma-glutamyl transferase (GGT), total bilirubin, conjugated bilirubin, cholinesterase, total cholesterol, high-density lipoprotein (HDL) cholesterol, triglyceride, glucose, amylase, creatine kinase, urea nitrogen, creatinine and uric acid. Alpha-fetoprotein and HBsAg were also measured. We studied the relationship between blood concentrations of total PCDFs and the items of the blood chemistry analysis, alpha-fetoprotein and HBsAg. Results: Of the 20 items of blood chemistry, alpha-fetoprotein and HBsAg, we found three items (GGT, HDL cholesterol and creatinine) were significantly related to the total PCDF level using three-way analysis of variance (ANOVA). Conclusion: A significant relationship between three items of the blood chemistry analysis (GGTP, HDL cholesterol and creatinine) and total PCDF levels in the blood was observed in 2002. The blood concentrations of total PCBs and PCDFs have now decreased; however, the PCDFs in patients with Yusho still affect blood chemistry.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2005.03.005
      Issue No: Vol. 1, No. 1 (2017)
  • Cardiac, pulmonary and renal function in Yusho patients
    • Authors: Yoichi Nakanishi; Shoji Tokunaga; Koichi Takayama; Kazuyoshi Kuwano
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): Yoichi Nakanishi, Shoji Tokunaga, Koichi Takayama, Kazuyoshi Kuwano
      Background: Thirty-five years after the Yusho incident, some symptoms, signs and laboratory abnormalities are still found in Yusho patients. Objective: To describe the cardiovascular, respiratory and renal abnormalities caused by Yusho, especially in relation to blood polychlorinated dibenzofuran concentration. Methods: A total of 358 officially registered patients with Yusho participated in this study. Medical records of the patients obtained from the annual nationwide health examinations held from 2001 to 2003 were used in the study. The symptoms, signs and laboratory findings in cardiac, respiratory and renal systems were compared with blood concentrations of polychlorinated biphenyls (PCBs) and 2,3,4,7,8-pentachlorodibenzofuran (PeCDF). Results: Airway symptoms such as cough and sputum were frequently seen in Yusho patients, whereas other symptoms, signs and laboratory abnormalities were not remarkable. There were marginal relationships between cough and blood concentration of PCBs, and between sputum and 2,3,4,7,8-PeCDF. Conclusion: Organs of the respiratory system remain affected by Yusho 35 years after the incident, whereas little effect on cardiac and renal systems is observed.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.jdermsci.2004.12.008
      Issue No: Vol. 1, No. 1 (2017)
  • Neurological signs and symptoms in patients with chronic PCB poisoning
           (Yusho accident) for more than 36 years
    • Authors: Hirokazu Furuya; Takeshi Yamada; Yasumasa Ohyagi; Koji Ikezoe; Tasuku Miyoshi; Naoki Fujii; Jun-ichi Kira
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): Hirokazu Furuya, Takeshi Yamada, Yasumasa Ohyagi, Koji Ikezoe, Tasuku Miyoshi, Naoki Fujii, Jun-ichi Kira
      Background: The existence of peripheral neuropathy after chronic exposure to polychlorinated biphenyls (PCBs) is still controversial because studies concerning the effects of PCBs on the peripheral nervous system are rare. Objective: The purpose of this study was to determine the correlation between neurological signs and symptoms and the concentration of serum PCBs. Materials and methods: Neurological data collected from the results of a nationwide health examination of 450 male and 557 female Yusho victims (chronic PCB poisoning) exposed more than 36 years ago were compared with recent measurements of the serum PCB concentration and patterns. Results: The frequency of sensory disturbance detected by neurological examination was significantly higher in the group of officially acknowledged victims (male, P =0.014; female, P =0.001) than in age-matched controls. Significant differences were not observed between the serum PCB patterns and the neurological findings, but the serum PCB concentration was significantly higher in the group with decreased tendon reflex in officially and non-officially acknowledged female Yusho victims (male, P =0.994; female, P =0.014). Conclusion: These results suggest that the long half-life of PCBs and their accumulation in fatty tissue can lead to persistent mild impairment of the peripheral nervous system even long after exposure.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2005.03.007
      Issue No: Vol. 1, No. 1 (2017)
  • Complete blood cell counts and blood chemistry in Yusho
    • Authors: Toshiro Yoshimura; Jiro Nakano; Minoru Okita; Yasuki Kikuchi; Takashi Kitamura; Takehiko Ishikawa
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): Toshiro Yoshimura, Jiro Nakano, Minoru Okita, Yasuki Kikuchi, Takashi Kitamura, Takehiko Ishikawa
      Background: Because of their lipophilic nature, polychlorinated biphenyls (PCBs) bioaccumulate in the food chain and their residues have been detected in foods. Consequently, they accumulate readily in the human body. Reports suggest that PCB blood levels remain constant or increase. Little, however, is known about the long-term hazardous effects of PCBs and dioxins on human health. Yusho is a type of food poisoning caused by PCBs and dioxins that contaminated rice bran oil. We analyzed blood samples of the Yusho patients from 1986 to 2002, and studied changes in blood cell counts, blood chemistry and tumor markers. Participants and methods: A population of 1041 patients was divided into patient and control groups based on the diagnostic criteria established for Yusho and participant's blood polychlorinated quarterphenyl (PCQ) levels. In total, 1666 blood and 1652 urine samples from 374 patients in the patient group (PCQ levels=0.1ppb), and 373 blood and 302 urine samples from 151 people in the control group (PCQ levels<0.02ppb) were analyzed. Blood levels of PCBs, PCQs, polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) were determined, and we analyzed their correlation with the data of complete blood cell counts, blood chemistry and urinalysis. Results and conclusion: Blood analyses, blood chemistry and urine values in Yusho patients were not significantly different from those in the control group 34 years after the Yusho incident. PCBs, PCQs or PCDFs may, however, affect hematogenesis, serum potassium, serum phosphorus, protein metabolism and creatine kinase metabolism because these parameters had slight but significant correlations with the levels of PCBs, PCQs or PCDFs. Exposure to PCBs and the related organochlorine compounds should be avoided.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.jdermsci.2004.12.010
      Issue No: Vol. 1, No. 1 (2017)
  • Ophthalmic findings in Yusho
    • Authors: Takahiko Nakamura; Miho Miyazaki; Yoshitaka Ohnishi; Tatsuro Ishibashi
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): Takahiko Nakamura, Miho Miyazaki, Yoshitaka Ohnishi, Tatsuro Ishibashi
      Background: The ocular signs in Yusho include hypersecretion by the meibomian glands, abnormal pigmentation of the bulbar conjunctiva, unusual pigmentation of the limbal conjunctiva, pigmentation of the tarsal conjunctiva and edema of the eyelid. Participants and methods: The ocular symptoms in Yusho patients were analyzed to investigate their relationship with the concentration of dioxins in the blood. The participants were patients with Yusho who underwent examinations including measurement of blood dioxin levels and ocular symptoms in 2002. Results and conclusion: The significant relation between the increase in ocular discharge and the level of polychlorinated dibenzofurans (PCDFs) in the blood is currently considered strong. No significant relationship with blood PCDF levels was found with any of the other four ocular symptoms. Although the blood levels of polychlorinated biphenyls (PCBs) and dioxins are now decreasing in Yusho patients, they still cause abnormal discharge from the eye.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2005.03.009
      Issue No: Vol. 1, No. 1 (2017)
  • Oral mucosa and dental findings in Yusho
    • Authors: Isamu Hashiguchi; Akifumi Akamine
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): Isamu Hashiguchi, Akifumi Akamine
      Background: Yusho is a disease caused by the ingestion of rice bran oil contaminated with polychlorinated biphenyls (PCBs), polychlorinated dibenzofurans (PCDFs) and related compounds. Oral lesions such as oral pigmentation, anomalies of the dental root shape and deficiency of tooth germs have been observed in Yusho patients. Objective: The purpose of this study was to determine the prevalence of oral lesions, especially oral pigmentation, in Yusho patients, and also to describe the relationship between oral lesions and PCBs or PCDFs. Methods: Visual and radiographic examinations were performed on Yusho patients who visited the dentist during the annual health examination in Fukuoka prefecture. The data obtained from 1968 to 2003 were analyzed. Results: Gingival pigmentation was the most common of all types of oral pigmentation seen in Yusho patients. Of all the examined Yusho patients, the proportion who had gingival pigmentation was more than 60% during the early phase after the Yusho incident, but this value had decreased to below 30% in 1993. However, it subsequently increased again to about 50% in 2003. Yusho patients with a blood PCB pattern typical of Yusho showed the highest incidence of oral pigmentation 5 years after PCB poisoning. As time passed, however, there was no specific difference in the prevalence of oral pigmentation between any type of PCB pattern, either specific to Yusho or commonly observed in the general population. Analysis of data by three-way analysis of variance (ANOVA) in 2001 and 2002 showed that there was a close relationship between the presence of upper gingival pigmentation and blood PCDF levels. Conclusions: The prevalence of oral pigmentation still remains high even after 35 years, and PCDFs may be responsible for the presence of oral pigmentation.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2005.03.010
      Issue No: Vol. 1, No. 1 (2017)
  • Dermatological manifestations in Yusho: correlation between skin symptoms
           and blood levels of dioxins, such as polychlorinated dibenzofurans (PCDFs)
           and polychlorinated biphenyls (PCBs)
    • Authors: T. Uenotsuchi; J. Nakayama; M. Asahi; O. Kohro; T. Akimoto; M. Muto; K. Shimizu; I. Katayama; T. Kanzaki; Y. Kanagawa; T. Imamura; M. Furue
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): T. Uenotsuchi, J. Nakayama, M. Asahi, O. Kohro, T. Akimoto, M. Muto, K. Shimizu, I. Katayama, T. Kanzaki, Y. Kanagawa, T. Imamura, M. Furue
      Background and objective: Yusho occurred in western Japan in 1968 and was caused by ingestion of rice bran oil that was contaminated with polychlorinated biphenyls (PCBs) and dioxins such as polychlorinated dibenzofurans (PCDFs). At that time, the skin symptoms presented by patients with Yusho were at their most prominent and worst severity. Analysis of blood to determine the concentration of dioxins started in 2001 in Fukuoka prefecture, and in 2002 the examination was performed throughout Japan. There have been no reports on the relationship between blood concentration of dioxins and skin symptoms in Yusho. This is the first report to examine the relationship between blood concentration of dioxins and skin symptoms in Yusho, using statistical analyses. Methods: Using the global skin severity grade, we analyzed the change in skin symptoms, which were examined at the annual medical check-up of patients with Yusho. We also investigated the relationship between the items of the annual dermatological examination and blood concentrations of total PCDFs and total PCBs. Results: The severity of skin symptoms improved significantly in the first 20 years; nowadays, however, further improvement can hardly be observed. Using three-way analysis of variance (ANOVA), we found that of the 21 items of the dermatological examination, nine were significantly related to total PCDFs, and five were related to total PCBs. Only one item was significantly related both to total PCDFs and total PCBs. Conclusion: More than 36 years have passed since the Yusho incident, and about 60% of the patients currently present with no skin symptoms. In contrast, in about 40% of the patients, characteristic skin symptoms of Yusho, such as pigmentation of skin, black comedones and acneform eruptions, could still be observed. Our analysis of the relationship between skin symptoms and blood concentrations of total PCDFs and total PCBs proves that not only PCBs but also PCDFs have an important role in the skin symptoms of Yusho.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.jdermsci.2004.12.016
      Issue No: Vol. 1, No. 1 (2017)
  • Sex ratio in the children of Yusho patients
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): T. Uenotsuchi, Y. Iio, S. Tadakuma, R. Haraduka, Y. Kanagawa, T. Imamura, M. Furue
      In some cases of intoxication with dioxins and dioxin-related compounds such as Yu-cheng, Seveso, Russian and Austrian chloracne cohorts, there was a significant reduction in the male-to-female sex ratio in children born to men who had been exposed to dioxins and dioxin-related compounds before age 20 years or in their early 20s. The aim of this study was to ascertain whether parental exposure to dioxin-related compounds at a particular age affected the sex of subsequent offspring in Yusho. Maternal exposure had no significant effect on the sex ratio of the offspring. The sex ratio of children born to men exposed before age 20, men exposed after age 20, or to parents who were both exposed were not significantly different from the expected sex ratio of 0.514.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.jdermsci.2004.12.011
      Issue No: Vol. 1, No. 1 (2017)
  • Relationship of clinical symptoms and laboratory findings with blood
           levels of PCDFs in patients with Yusho
    • Authors: Yoshiyuki Kanagawa; Tomoaki Imamura
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): Yoshiyuki Kanagawa, Tomoaki Imamura
      Background and objective: Since the Kanemi Yusho poisoning incident, patients with Yusho have been followed up for 35 years in annual health examinations for Yusho symptoms by a national Study Group for Yusho. Because of recent advances in the technology for the measurement of dioxins, the determination of blood polychlorinated dibenzofuran (PCDF) levels has become possible with high accuracy. Thus, the purpose of this study was to investigate the relationship between clinical symptoms and dioxins, one of the causal agents, in patients with Kanemi Yusho oil poisoning disease. Methods: The participants were patients with oil poisoning disease who had undergone general examinations including measurement of PCDF levels, internal medicine, examination sheet (biochemistry, hematology), and dermatological, dental and ophthalmological examinations in 2001 and 2002. We investigated the presence or absence of symptoms in these examinations and the relationship with PCDF levels by methods such as three-way analysis of variance (ANOVA). Results: Large differences were found between the examination results in 2001 and those in 2002. Items for which the relationship between the symptoms or the results and PCDF levels was currently considered strong were polychlorinated biphenyl (PCB)-related items, and items of a gingival nature and gingival sites.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.jdermsci.2004.12.013
      Issue No: Vol. 1, No. 1 (2017)
  • The concepts of the new criteria for Yusho poisoning
    • Authors: Shoji Tokunaga; Takao Iida; Masutaka Furue
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): Shoji Tokunaga, Takao Iida, Masutaka Furue
      Background: The current diagnostic criteria for Yusho poisoning do not include dioxin levels, although one of the subgroups of dioxins, polychlorinated dibenzofurans, was shown to contribute the most to the total toxic equivalent quantity in the blood of Yusho patients. Objective: To propose new diagnostic criteria for Yusho using blood dioxin levels. Subjects and methods: Participants of the nationwide health examination for Yusho in 2001 and 2002, and randomly selected residents of Fukuoka City, Japan, were included in this study. A multiplicative model was applied to blood 2,3,4,7,8-pentachlorodibenzofuran (PeCDF) level with age and sex as explanatory variables. A logistic regression model including 2,3,4,7,8-PeCDF level, age and sex was also used. Results: Three criteria are proposed based on different approaches: 2,3,4,7,8-PeCDF level adjusted for age and sex (criterion 1), its one-tailed upper prediction limit (criterion 2), and the estimated probability of being a Yusho patient (criterion 3). By applying these three criteria to potential victims who had not been diagnosed as having Yusho according to the current diagnostic criteria, the same people were identified as Yusho sufferers. Criterion 1 with an upper 99 percentile of age- and sex-adjusted 2,3,4,7,8-PeCDF level of controls as a cut-off was determined, from a practical perspective, to be superior to the other criteria.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.jdermsci.2004.12.014
      Issue No: Vol. 1, No. 1 (2017)
  • Effects of dioxins on stress-responsive systems and their relevance to
    • Authors: Takumi Ishida; Miho Hori; Yuji Ishii; Kazuta Oguri; Hideyuki Yamada
      Abstract: Publication date: April 2005
      Source:Journal of Dermatological Science Supplement, Volume 1, Issue 1
      Author(s): Takumi Ishida, Miho Hori, Yuji Ishii, Kazuta Oguri, Hideyuki Yamada
      Background: Dioxins and related compounds, exemplified by 2,3,7,8-tetrachlorodibenzo-p-dioxin, are recognized as widespread, persistent and highly toxic environmental pollutants. Although numerous studies have been performed to clarify the mechanisms governing dioxin toxicity, these are not yet fully understood because of their complexity. In 1968, subacute poisoning by polychlorinated biphenyls, called ‘Yusho’, occurred in the southwest part of Japan. Although many of the Yusho patients appear to be free from any of the symptoms produced by the pollutant at present, they remain at high risk of dioxin toxicity because of the high concentrations present in the body. To date, no effective method for combating this toxicity has been developed. Objective: In this review, we summarize dioxin toxicity by focusing on the quenching systems of reactive oxygen species and chaperone proteins. In addition, the possibility of the development of protective and therapeutic treatments for dioxin toxicity is discussed.

      PubDate: 2017-12-12T13:08:34Z
      DOI: 10.1016/j.descs.2005.03.011
      Issue No: Vol. 1, No. 1 (2017)
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