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DERMATOLOGY AND VENEREOLOGY (164 journals)                     

Showing 1 - 164 of 164 Journals sorted alphabetically
Acta Dermato-Venereologica     Open Access   (Followers: 12)
Acta Dermatovenerologica Croatica     Open Access   (Followers: 1)
Actas Dermo-Sifiliograficas     Full-text available via subscription   (Followers: 3)
Actas Dermo-Sifiliográficas (English Edition)     Full-text available via subscription   (Followers: 2)
Advances in Dermatology     Full-text available via subscription   (Followers: 15)
Advances in Skin & Wound Care     Hybrid Journal   (Followers: 30)
African Journal of AIDS Research     Hybrid Journal   (Followers: 8)
AIDS     Hybrid Journal   (Followers: 24)
AIDS Care: Psychological and Socio-medical Aspects of AIDS/HIV     Hybrid Journal   (Followers: 10)
AIDS Patient Care and STDs     Hybrid Journal   (Followers: 3)
AIDS Research and Human Retroviruses     Hybrid Journal   (Followers: 9)
AIDS Research and Therapy     Open Access   (Followers: 15)
AIDS Research and Treatment     Open Access   (Followers: 2)
Aktuelle Dermatologie     Hybrid Journal   (Followers: 7)
Allergo Journal     Full-text available via subscription   (Followers: 2)
American Journal of Clinical Dermatology     Full-text available via subscription   (Followers: 26)
American Journal of Dermatopathology     Hybrid Journal   (Followers: 18)
Anais Brasileiros de Dermatologia     Open Access   (Followers: 2)
Anaplastology     Open Access  
Annales de Dermatologie et de Vénéréologie     Full-text available via subscription  
Archives de Pédiatrie     Full-text available via subscription  
Archives de sciences sociales des religions     Open Access   (Followers: 1)
Archives des Maladies du Coeur et des Vaisseaux - Pratique     Hybrid Journal  
Archives of Dermatological Research     Hybrid Journal   (Followers: 7)
Archives of Gerontology and Geriatrics     Hybrid Journal   (Followers: 13)
Archives of Industrial Hygiene and Toxicology     Open Access   (Followers: 8)
Archives of Medical Research     Hybrid Journal   (Followers: 3)
Archives of Physical Medicine and Rehabilitation     Hybrid Journal   (Followers: 58)
Archivio di Ortopedia e Reumatologia     Hybrid Journal  
Asian Journal of Dermatology     Open Access   (Followers: 2)
ästhetische dermatologie & kosmetologie     Full-text available via subscription  
Australasian Journal of Dermatology     Hybrid Journal   (Followers: 8)
Berkala Ilmu Kesehatan Kulit dan Kelamin / Periodical of Dermatology and Venereology     Open Access  
Biomedical Dermatology     Open Access  
BMC Dermatology     Open Access   (Followers: 13)
BMJ Sexual & Reproductive Health     Hybrid Journal   (Followers: 2)
British Journal of Dermatology     Hybrid Journal   (Followers: 54)
Case Reports in Dermatological Medicine     Open Access   (Followers: 2)
Case Reports in Dermatology     Open Access   (Followers: 9)
Clinical and Experimental Dermatology     Hybrid Journal   (Followers: 13)
Clinical Dermatology Review     Open Access   (Followers: 5)
Clinical Skin Cancer     Full-text available via subscription  
Clinical, Cosmetic and Investigational Dermatology     Open Access   (Followers: 10)
Clinics in Dermatology     Hybrid Journal   (Followers: 14)
Contact Dermatitis     Hybrid Journal   (Followers: 8)
Cosmetics     Open Access   (Followers: 5)
Current Dermatology Reports     Hybrid Journal   (Followers: 7)
Current Fungal Infection Reports     Hybrid Journal   (Followers: 5)
Current HIV Research     Hybrid Journal   (Followers: 7)
Current HIV/AIDS Reports     Hybrid Journal   (Followers: 6)
Current Sexual Health Reports     Hybrid Journal   (Followers: 3)
Cutaneous and Ocular Toxicology     Hybrid Journal   (Followers: 10)
Der Hautarzt     Hybrid Journal   (Followers: 2)
Dermatitis     Hybrid Journal   (Followers: 1)
Dermato-Endocrinology     Open Access   (Followers: 2)
Dermatología Venezolana     Open Access  
Dermatologic Clinics     Full-text available via subscription   (Followers: 4)
Dermatologic Reviews     Hybrid Journal  
Dermatologic Surgery     Hybrid Journal   (Followers: 9)
Dermatologic Therapy     Hybrid Journal   (Followers: 2)
Dermatologica Sinica     Open Access  
Dermatological Nursing     Full-text available via subscription   (Followers: 2)
Dermatology     Full-text available via subscription   (Followers: 20)
Dermatology and Cosmetic     Open Access   (Followers: 8)
Dermatology and Therapy     Open Access   (Followers: 4)
Dermatology Online Journal     Open Access   (Followers: 1)
Dermatology Reports     Open Access   (Followers: 3)
Dermatology Research and Practice     Open Access   (Followers: 4)
Dermatology Times     Free  
Dermatopathology     Open Access   (Followers: 3)
Egyptian Journal of Dermatology and Venerology     Open Access   (Followers: 1)
EMC - Cosmetologia Medica e Medicina degli Inestetismi Cutanei     Full-text available via subscription  
EMC - Dermatología     Full-text available via subscription   (Followers: 1)
European Journal of Dermatology     Hybrid Journal   (Followers: 15)
Experimental Dermatology     Hybrid Journal   (Followers: 10)
Expert Review of Dermatology     Hybrid Journal   (Followers: 15)
Forum Dermatologicum     Hybrid Journal  
Graefe's Archive for Clinical and Experimental Ophthalmology     Hybrid Journal   (Followers: 9)
Güncel Dermatoloji Dergisi     Open Access  
HautinForm     Full-text available via subscription  
hautnah     Hybrid Journal  
hautnah dermatologie     Hybrid Journal  
HIV & AIDS Review     Full-text available via subscription   (Followers: 13)
HIV Clinical Trials     Hybrid Journal   (Followers: 5)
HIV Medicine     Hybrid Journal   (Followers: 3)
Indian Dermatology Online Journal     Open Access   (Followers: 3)
Indian Journal of Dermatology     Open Access   (Followers: 2)
Indian Journal of Dermatology, Venereology and Leprology     Open Access   (Followers: 4)
Indian Journal of Dermatopathology and Diagnostic Dermatology     Open Access  
Indian Journal of Drugs in Dermatology     Open Access   (Followers: 1)
Indian Journal of Paediatric Dermatology     Open Access   (Followers: 2)
Indian Journal of Sexually Transmitted Diseases and AIDS     Open Access   (Followers: 2)
International Archives of Medicine     Open Access   (Followers: 3)
International Journal of Dermatology     Hybrid Journal   (Followers: 14)
International Journal of Dermatology and Clinical Research     Open Access   (Followers: 2)
International Journal of Research in Dermatology     Open Access   (Followers: 1)
International Journal of STD & AIDS     Hybrid Journal   (Followers: 7)
International Journal of Women's Dermatology     Open Access   (Followers: 1)
International STD Research & Reviews     Open Access   (Followers: 1)
JAAD Case Reports     Open Access   (Followers: 1)
JAIDS : Journal of Acquired Immune Deficiency Syndromes     Hybrid Journal   (Followers: 4)
JAMA Dermatology     Full-text available via subscription   (Followers: 49)
JAMA Facial Plastic Surgery     Full-text available via subscription   (Followers: 13)
JMIR Dermatology     Open Access   (Followers: 1)
Journal of AIDS & Clinical Research     Open Access   (Followers: 3)
Journal of Clinical & Experimental Dermatology Research     Open Access   (Followers: 6)
Journal of Clinical and Investigative Dermatology     Open Access   (Followers: 2)
Journal of Cosmetic Dermatology     Hybrid Journal   (Followers: 11)
Journal of Cosmetics, Dermatological Sciences and Applications     Open Access   (Followers: 7)
Journal of Cutaneous Immunology and Allergy     Open Access  
Journal of Cutaneous Medicine and Surgery     Full-text available via subscription  
Journal of Dermatological Research     Open Access  
Journal of Dermatological Science     Hybrid Journal   (Followers: 2)
Journal of Dermatological Science Supplement     Full-text available via subscription   (Followers: 1)
Journal of Dermatological Treatment     Hybrid Journal   (Followers: 2)
Journal of Dermatology & Dermatologic Surgery     Open Access   (Followers: 1)
Journal of General-Procedural Dermatology & Venereology Indonesia     Open Access  
Journal of HIV/AIDS & Social Services     Hybrid Journal   (Followers: 9)
Journal of Investigative Dermatology     Hybrid Journal   (Followers: 28)
Journal of Investigative Dermatology Symposium Proceedings     Full-text available via subscription  
Journal of Sexual Medicine     Hybrid Journal   (Followers: 6)
Journal of Sexually Transmitted Diseases     Open Access   (Followers: 3)
Journal of Skin and Stem Cell     Open Access   (Followers: 3)
Journal of Skin Cancer     Open Access   (Followers: 3)
Journal of Surgical Dermatology     Open Access   (Followers: 1)
Journal of the American Academy of Dermatology     Full-text available via subscription   (Followers: 37)
Journal of the Dermatology Nurses' Association     Hybrid Journal   (Followers: 3)
Journal of the Egyptian Women’s Dermatologic Society     Partially Free  
Journal of the European Academy of Dermatology and Venereology     Hybrid Journal   (Followers: 15)
Journal of the International AIDS Society     Open Access   (Followers: 10)
Journal of the Saudi Society of Dermatology & Dermatologic Surgery     Open Access   (Followers: 1)
Karger Kompass Dermatologie     Full-text available via subscription  
Karger Kompass Pneumologie     Full-text available via subscription   (Followers: 1)
Langenbeck's Archives of Surgery     Hybrid Journal   (Followers: 4)
Medical and Surgical Dermatology     Hybrid Journal   (Followers: 1)
Medical Mycology     Open Access   (Followers: 4)
Nepal Journal of Dermatology, Venereology & Leprology     Open Access   (Followers: 1)
Neurobehavioral HIV Medicine     Open Access   (Followers: 2)
OA Dermatology     Open Access   (Followers: 1)
Open AIDS Journal     Open Access  
Open Dermatology Journal     Open Access  
Perspectives On Sexual and Reproductive Health     Hybrid Journal   (Followers: 7)
Pigment International     Open Access   (Followers: 1)
Psoriasis : Targets and Therapy     Open Access   (Followers: 4)
Revista Internacional de Ciencias Podológicas     Open Access  
SAHARA : Journal of Social Aspects of HIV / AIDS Research Alliance     Open Access   (Followers: 5)
Scars, Burns & Healing     Open Access  
Serbian Journal of Dermatology and Venereology     Open Access   (Followers: 1)
Sex Education: Sexuality, Society and Learning     Hybrid Journal   (Followers: 5)
Sexual & Reproductive Healthcare     Hybrid Journal   (Followers: 2)
Sexual Health     Hybrid Journal   (Followers: 4)
Sexually Transmitted Diseases     Hybrid Journal   (Followers: 6)
Sexually Transmitted Infections     Hybrid Journal   (Followers: 6)
Skin Appendage Disorders     Full-text available via subscription   (Followers: 1)
Skin Pharmacology and Physiology     Full-text available via subscription   (Followers: 7)
Skin Research and Technology     Hybrid Journal   (Followers: 6)
Southern African Journal of HIV Medicine     Open Access   (Followers: 3)
Sri Lanka Journal of Sexual Health and HIV Medicine     Open Access  
Studies in Gender and Sexuality     Hybrid Journal   (Followers: 21)
Surgical & Cosmetic Dermatology     Open Access   (Followers: 3)
The Journal of Dermatology     Hybrid Journal   (Followers: 4)
The Rose Sheet     Full-text available via subscription   (Followers: 2)
Vestnik dermatologii i venerologii     Open Access  
Veterinary Dermatology     Hybrid Journal   (Followers: 8)

           

Similar Journals
Journal Cover
Journal of Investigative Dermatology
Journal Prestige (SJR): 2.282
Citation Impact (citeScore): 4
Number of Followers: 28  
 
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 0022-202X - ISSN (Online) 1523-1747
Published by Elsevier Homepage  [3207 journals]
  • Late-Breaking Abstract Keyword List
    • Abstract: AcneLB938, LB946
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.076
      Issue No: Vol. 140, No. 7 (2020)
       
  • Late-Breaking Abstracts Author
    • Abstract: Alakel, AkramLB950
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.075
      Issue No: Vol. 140, No. 7 (2020)
       
  • Late-Breaking Abstracts Title Page
    • Abstract: Society for Investigative Dermatology 2020
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.074
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB974 Donor-derived T cells accumulate in skin during resolution of acute
           GVHD
    • Authors: L. Khatib; E. Schunkert, P. Hsieh, S.J. Divito
      Abstract: Graft-versus-host disease is a significant cause of morbidity and mortality following stem cell transplantation. Donor T cells are believed to be the main mediators of disease, however we recently identified that host T cells survive stem cell transplant conditioning and are present in high percentages in skin during active acute GVHD. Host T cells appear to contribute to disease pathology in both human studies and a humanized mouse model. Data further suggested that after resolution of acute GVHD, T cell chimerism in skin shift dramatically to donor T cells.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.073
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB973 Immunologic profiling of fixed drug eruptions
    • Authors: E. Schunkert; G. Romar, P. Hsieh, R. Foreman, V. Barrera, S.J. Divito
      Abstract: Fixed drug eruptions (FDE) are a unique form of drug reaction in which a rash recurs at the same skin site(s) with each repeated drug exposure. This intriguing reaction has been postulated to be due to skin resident memory T cells, yet investigation into T cell phenotype and function and the concomitant inflammatory milieu are surprisingly limited. To elucidate disease pathobiology, we performed Nanostring transcriptional profiling on RNA extracted from formalin fixed paraffin embedded skin specimens of active FDE lesions and healthy control skin.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.072
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB972 IL-17 inhibitor-induced remission of guttate psoriasis
    • Authors: A. Fogel; B. Strober
      Abstract: Effective, targeted treatments for guttate psoriasis are lacking. Drugs that block the interleukin-17 (IL-17) pathway are highly effective in the treatment of plaque psoriasis. Given the overlap of immunopathogenic features between guttate and plaque psoriasis, including an over activation of Th17 cells, it is plausible that IL-17A inhibition would be an effective treatment for guttate psoriasis. Ixekizumab, a humanized IgG4 monoclonal antibody that targets interleukin-17A (IL-17A) is FDA-approved for the treatment of moderate-to-severe plaque psoriasis in adult patients who are candidates for systemic therapy, but to our knowledge has never been reported in the treatment of guttate psoriasis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.071
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB971 Type I and II interferon signaling differentially associated with
           histopathologic findings in dermatomyositis skin
    • Authors: T. Wu; M. Tabata, L. Mushila Hodgkinson, K. Page, C. Huard, J. Buhlmann, K. Sarin, D. Fiorentino
      Abstract: Upregulation of interferon (IFN) signaling is well documented in the skin, as well as blood and muscle of dermatomyositis (DM) patients. However, it is unclear if IFN signaling is related to the pathologic features commonly found in DM skin biopsies. Here, we investigate the association of Type I and II IFN gene signatures with cardinal histologic findings seen in DM skin to better understand the contribution of IFN signaling to key pathological features of DM. Histological staining and RNA sequencing were performed on 113 skin biopsies from 99 patients with DM.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.070
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB970 Dermoscopy during treatment of early Mycosis Fungoides
    • Authors: G. Chaidemenos; T. Spiliopoulos, E. Balaskas, G. Kontochristopoulos
      Abstract: The type of dermoscopy pattern in post-therapy remaining plaques of early Mycosis Fungoides, might help physicians in selecting the most appropriate therapeutic measures. Dermoscopy is a noninvasive popular technique enhancing the diagnostic ability in Derrmatological practice. “Spermatozoa-like” structures and fine short linear vessels are the most specific dermoscopic diagnostic features of early Mycosis Fungoides. Little is known about their modification following treatment. In a pilot study we prospectively investigated the modifications, if any, of dermoscopy features in 8 patients, who had been treated for at least 1 year.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.069
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB969 Analysis of epidermal gene expression profiles in patients with
           allergic contact dermatitis using non-invasive skin tape stripping
    • Authors: I. Tam; J. Park, J. Yu
      Abstract: Allergic contact dermatitis (ACD) affects 20% of patients and combined with irritant contact dermatitis (ICD) account for more than 90% of occupational skin diseases. Patch testing is the gold standard for diagnosis of ACD but result interpretation may be patient and physician dependent. The purpose of this study is to examine whether noninvasive tape stripping can be used to differentiate ACD, ICD, and normal skin. We examined 39 immune and barrier genes expressed in various skin layers. Patients referred to the Massachusetts General Hospital Contact Dermatitis Clinic with confirmed diagnosis of ACD through patch testing were recruited.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.068
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB968 Inhibition of sonic hedgehog signalling via MAPK activation controls
           chemotherapy-induced alopecia
    • Authors: I.S. Haslam; G. Zhou, G. Xie, X. Teng, X. Ao, Z. Yan, E. Smart, D. Rutkowski, J. Wierzbicka, Y. Zhou, Z. Huang, Y. Zhang, N. Farjo, B. Farjo, R. Paus, Z. Yue
      Abstract: Chemotherapy-induced hair loss (alopecia, CIA) remains a major unsolved problem in clinical oncology. CIA is often considered to be a consequence of the anti-mitotic and apoptosis-promoting properties of chemotherapy drugs acting on rapidly proliferating hair matrix keratinocytes. Here we show that in a mouse model of CIA, down-regulation of Shh signalling in the hair matrix is a critical early event. Inhibition of Shh signalling recapitulated key morphological and functional features of CIA, whereas recombinant Shh protein partially rescued hair loss.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.066
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB967 Hemorrhagic complications in outpatient dermatological surgery
    • Authors: A. Chandra; M. Manway, G. Egnatios
      Abstract: The increasing incidence of cutaneous neoplasms in the Caucasian population of the United States has made Mohs micrographic surgery (MMS) and surgical excision with wide margins the standard of care for the treatment of melanoma and non-melanoma skin cancers. These commonly performed surgical modalities promote high cure rates and conservative treatment methods, sparing the maximal amount of normal tissue. Although there is a growing body of evidence regarding the efficacy of dermatological surgery for the removal of cutaneous malignancies, there is still some debate regarding the guidelines for maintaining safety and avoiding complications of MMS and other dermatologic procedures.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.065
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB966 Transcriptional regulation of migrating-keratinocyte adhesion during
           cutaneous wound healing
    • Authors: G. Kashgari; S. Venkatesh, S. Gacias, A. Bayat, R. Herndon Klein, A. Paul Ta, R. Ramos, M.V. Plikus, P.H. Wang, B. Andersen
      Abstract: The migration of wound-edge keratinocytes is part of the wound response, crucial for complete wound closure. Despite significant advances, the molecular mechanisms that orchestrate cell-cell adhesion between migrating keratinocytes are not fully characterized. During wound re-epithelization, keratinocytes at the wound edge undergo series of cellular modifications. These cellular modifications require a loosening of cell-cell adhesion for effective migration. Mice lacking the epidermal transcription factor Grainyhead Like-3 (GRHL3) exhibit impaired wound healing and an increased adhesion between keratinocytes at the wound edge.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.064
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB965 Follicular trochanters: A potential mechanism for stem cell
           depletion in scarring alopecia
    • Authors: F.C. Roche; A. Fischer, K.J. Gaddis, T. Dentchev, S.C. Taylor, J.T. Seykora
      Abstract: The follicular bulge is a stem cell niche where the outer root sheath (ORS) abuts the arrector pili muscle insertion site in both murine and human hair follicles. Prior studies have recognized an uncommon structure termed the “follicular trochanter” which was described as an epithelial protrusion of the outer root sheath in anagen follicles. It has been shown that these structures prominently express keratin 15, a stem cell marker. We sought to determine if trochanters embedded in the fibrotic stroma contribute to follicular scarring in cicatricial alopecias.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.062
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB963 Perceptions of clinical trials among patients with atopic
           dermatitis: An analysis by race
    • Authors: A. Bazen; A. Sevagamoorthy, F.K. Barg, J. Takeshita
      Abstract: There is a lack of diversity among participants in dermatologic clinical trials. In order to gain an understanding of reasons for poor racial/ethnic diversity in dermatologic clinical trials, we performed a qualitative pilot study of adults with atopic dermatitis (AD) to identify their perceptions of clinical trials. We performed semi-structured interviews of 26 adults with AD. We used a purposive sampling approach to enroll a similar number of white (N=8), black (N=8), and Asian (N=9) adults. Interview responses were independently coded by two research assistants using a grounded theory approach.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.059
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB962 Evaluation of time of presentation of central centrifugal
           cicatricial alopecia and outcomes of disease
    • Authors: T. Onamusi; A. McMichael
      Abstract: Background: Central centrifugal cicatricial alopecia (CCCA) is a form of scarring hair loss that predominantly affects women of African descent. Although the etiology remains unknown, the pathogenesis involves inflammatory mediated destruction of the hair follicles. The management of CCCA is approached symptomatically rather than through evidence-based recommendations. Since treatment options do not provide a cure and are only aimed at halting disease progression, it is important to understand how time to presentation and symptomatology relates to treatment outcomes.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.058
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB961 Disparities in dermatology referral and consultation patterns in the
           emergency department
    • Authors: R. Kwak; D. Bartenstein, D. Rubins, M.H. Noe, A.P. Charrow
      Abstract: While the Emergency Department (ED) frequently places outpatient follow-up referrals and in-ED consultations to dermatology, little is currently known about whether demographic factors such as race and sex impact these rates. Research in other contexts such as admission to cardiology has already demonstrated ED triage is impacted by race when controlled for other factors. We performed a retrospective chart review of all patients seen in the ED at two university hospitals between 1/1/2017 and 7/1/2019 for rash or dermatitis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.057
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB960 Oncodermatologic conditions in skin of color
    • Authors: B. Olamiju; J.S. Leventhal
      Abstract: Purpose: Dermatologic toxicities from anticancer treatments are an increasing challenge for patients and their providers. Effective prevention and clinical management of such toxicities can allow patients to remain on life-saving oncotherapeutics. The purpose of this study was to perform an analysis of cutaneous toxicities in skin of color patients that were referred to an oncodermatology clinic at a single academic institution. Methods: A chart review was conducted to investigate dermatologic toxicities in patients with skin of color on cancer treatment who were referred over the course of three years.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.056
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB959 Lesion location for melanoma pathology analysis
    • Authors: P. Xie; F. Li, S. Zhao, Q. Li, J. Liu, K. Lu, Y. Zhang, T. Li, J. Zhou, Z. Ke, X. Chen
      Abstract: Pathological imaging is the gold standard for the diagnosis of melanoma.Recent studies have shown that convolutional neural networks(CNNs) is effective on the diagnosis of melanoma from pathological images. However, CNN could only predict whether it was a melanoma, not locate the lesion. In this paper, we propose lesion location class activation mapping (LLCAM) method to precisely locate the lesion of melanoma in pathological images. First, we collect more than 300 skin digital whole-slide images, including 188 melanomas.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.054
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB958 Imiquimod treatment of lentigo maligna with positive margins, close
           margins, or field of dysplasia
    • Authors: R. Kwak; C. Joyce, A. Werchniak, J. Lin, H. Tsibris
      Abstract: Lentigo maligna (LM) is a subtype of melanoma in situ more commonly found in sun-exposed areas. LM can occur with an extension of atypical melanocytes into the periphery known as the “field effect” that makes surgical resection alone challenging.1 For this reason, primary or adjuvant imiquimod therapy is commonly practiced in treating LM. Though its efficacy is evident in the literature, little is currently known about its efficacy in treating the cases with positive (PM) or close margins (CLM) or with field of dysplasia (FD).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.053
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB957 Skin cancer recognition for whole slide histology images with
           state-of-the-art Convolutional Neural Networks
    • Authors: P. Xie; F. Li, S. Zhao, Q. Li, J. Liu, K. Lu, Y. Zhang, T. Li, J. Zhou, Z. Ke, X. Chen
      Abstract: Whole Slide histology image remains the gold standard. But recent research reveals a high discordance between individual pathologists. In order to reduce the misdiagnosis made by doctors, the aim of this study is to using deep learning to assist pathologists for skin cancers diagnosis. 626 whole slide images were collected (162 melanoma, 115 basal cell carcinoma, 349 nevus) from Xiangya Hospital. All the lesions in the WSIs were selected and random sampled as patches. The resulting patches of 500 WSIs were used for the training of a convolutional neural network (CNN).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.052
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB956 Adherence to adjuvant therapy in patients (pts) with resected
           melanoma
    • Authors: C. Beisel; T. Poretta, M. Burke, H. Sipsma, E. Fuqua, B. Stwalley, M. Yang
      Abstract: Treatment adherence can be impacted by several factors, including route of administration, dosing schedule, and pt perceptions. This study assessed pt-reported adherence and associated barriers in pts with melanoma treated with adjuvant therapy. Adult pts with resected stage 3/4 melanoma with no evidence of disease were recruited by a pt panel and the Melanoma Research Foundation to participate in an online survey. Pts were required to have received adjuvant therapy with nivolumab or pembrolizumab (intravenous [IV] cohort) or dabrafenib+trametinib (oral cohort).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.051
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB955 Predicting metastatic melanoma from melanoma pathological images
           using a Convolutional Neural Network: A Multicenter Study
    • Authors: P. Xie; F. Li, S. Zhao, Q. Li, J. Liu, K. Lu, Y. Zhang, T. Li, J. Zhou, z. ke, X. Chen
      Abstract: Pathological images are the gold standard for the diagnosis of melanoma.Recent studies have shown that convolutional neural networks(CNNs)' ability to diagnose melanoma from pathological images is as good as or slightly better than that of pathologists.However, few studies have verified whether CNN can directly predict the metastasis of melanoma from pathological images, which is an experiment of great medical significance. In the present study, We investigated whether CNNs can predict the metastasis of melanoma from pathological images.First, We collected 472 digital whole-slide images (metastatic melanoma 143, primary melanoma 329) to develop the model.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.050
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB954 Identification and characterization of patients with vitiligo
           treated with ruxolitinib cream based on the achievement of F-VASI50
    • Authors: M. Howell; H. Liu, S. Owens, K. Sun, F. Kuo, K. Butler
      Abstract: Vitiligo is a chronic, inflammatory skin disease characterized by increased interferon-gamma signaling through Janus kinase (JAK) 1 and JAK2 and subsequent activation of CD8+ T cells, which target melanocytes resulting in areas of depigmentation. Ruxolitinib cream, a JAK1/JAK2 inhibitor, is under investigation for vitiligo treatment in a 52-week, randomized, double-blind, phase 2 study (NCT03099304). Significantly more patients treated with ruxolitinib cream vs vehicle achieved ≥50% improvement in facial Vitiligo Area Scoring Index (FVASI50) at Week 24 (primary endpoint); at Week 52, patients treated with ruxolitinib cream 1.5% twice daily (BID) attained the highest FVASI50 response (57.6%).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.049
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB953 Melanoma cell invasion is inhibited in the absence of α3β1
           integrin mediated paracrine signaling from keratinocytes
    • Authors: K.J. Bommareddy; A. Dabaghian, A. Ndoye, L. Davis, C. DiPersio
      Abstract: Our previous work established a critical role for the laminin-binding integrin α3β1 in regulating the ability of epidermal keratinocytes to modify the cutaneous microenvironment during skin development, tumorigenesis and wound healing. These modifications occur through direct modulation of extracellular matrix (ECM) and paracrine signals to stromal cells (e.g., endothelial cells, fibroblasts) that facilitate angiogenesis, inflammation, or ECM remodeling. Mass spectrometry of the keratinocyte secretome identified a number of α3β1-dependent secreted proteins that modulate the microenvironment including ECM proteins (e.g., fibulin-2), proteases (e.g., MMP-9), and growth factors/cytokines (e.g., MRP-3).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.048
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB952 Multi-scale network for melanoma histopathology image analysis
    • Authors: P. Xie; F. Li, S. Zhao, Q. Li, J. Liu, K. Lu, Y. Zhang, T. Li, J. Zhou, z. ke, X. Chen
      Abstract: Histopathology images have distinct histomorphological features on different scales. In the pathological image analysis, pathologists can only make proper diagnosis by comprehensively analyzing histomorphological features of dif-ferent scales. However, the current convolutional neural network with single-scale image input does not have the powerful ability to merge features of different scales. Accordingly, we propose a multi-scale convolutional network architecture for merging features of different scale images, with refer-ence to the doctors’ diagnosis process.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.047
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB951 Interpretable pathologist-level classification of melanoma disease
           pathologies with a convolutional neural network pipeline
    • Authors: P. Xie; F. Li, S. Zhao, J. Liu, K. Lu, Y. Zhang, z. ke, X. Chen
      Abstract: For diagnosing melanoma, hematoxylin and eosin (H&E) stained tissue slides remains the gold standard. Accurate diagnosis of melanoma pathology images plays an important role in early diagnosis and successful treatment for melanoma. Deep convolutional neural networks (CNNs) show strong discrimination power for complex medical image recognition tasks. However,the black box nature of CNNs limits their role in clinical settings, where interpretable diagnosis is indispensable. In this paper, we build a deep learning pipeline to precise diagnosis of melanoma using CNNs and localize possible malignant melanoma tissue by the gradient-weighted class activation mapping (Grad-CAM).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.046
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB950 Geographic variations in cutaneous malignant melanoma distribution
           in Russian Federation
    • Authors: A. Muntyanu; E. Savin, F. Ghazawi, A. Alakel, A. Zubarev, I. Litvinov
      Abstract: Cutaneous Malignant Melanoma (CMM) incidence has been rising around the world and over the last three decades at rates greater than for any other malignancy. Our objective was to describe geographic trends in incidence and mortality of CMM in Russia between 2001 and 2017 using geo-informatics technique (mapping) and descriptive statistical analysis. Additionally, we aimed to study the associations between ethnicity, geographic latitude/longitude and CMM incidence/mortality rates. We retrospectively analyzed the data from the Moscow Oncology Research Institute, Ministry of Health of Russian Federation for the period of the study.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.045
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB949 Development of topical MEK inhibitor, NFX-179, as a chemopreventive
           agent for squamous cell carcinoma
    • Authors: A. Shah; B. Sell, W. Sun, M. Duncton, J. Banoo, J. Kincaid, K. Sarin, K.Y. Tsai
      Abstract: Cutaneous squamous cell carcinoma (cSCC) is the second most common skin cancer comprising over one million cases and contributing up to 9,000 deaths annually in the United States. We recently demonstrated that blockade of RAS signaling by MEK inhibitors reduces the formation of cSCCs in mice highlighting the potential of MEK inhibitors in chemoprevention of cSCC. However, the systemic toxicities of MEK inhibitors preclude their chronic usage in chemoprevention. To that end, NFlection Therapeutics has developed a topically formulated, novel and potent MEK inhibitor, NFX-179, designed to selectively inhibit MEK in SCC tumor tissue but with a high rate of clearance from plasma to limit systemic exposure.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.043
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB948 Evaluation of creams containing ozonated sunflower oil
    • Authors: P. Brizzi; M. Andreassi, L. Furi, J. Zanardi, V. Travagli
      Abstract: This study concerns the evaluation from the physical point of view of preparations for topical application containing sunflower oil (Helianthus annuus) as such and after ozonation. Six different creams with different amounts of either untreated or ozonated sunflower oil (3%, 5% and 10%) with respect to the original one without sunflower oil have been evaluated. Two different preparation methods have been adopted: i) adding the relative percentages of oil in the base cream; ii) replacing a part of a lipophilic component of the base cream.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.042
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB947 Development of LY3454738, an agonistic antibody to human CD200R
    • Authors: S.C. Potter; K.D. Werle, S.P. Bauer, D.I. Ruiz, J. Rhoden, S. Demarest, D. Witcher, A. Koester
      Abstract: CD200R is an immune receptor of the IgG family that is primarily expressed on cells of the myeloid lineage and was recently identified as a marker for Th2 biology (Blom et al 2017). In vivo studies with knockout mice of either the receptor or its ligand, CD200, have demonstrated that it is an inhibitory receptor capable of negatively regulating immune responses. Previous work using agonistic antibodies to mouse CD200R showed inhibition of mast cell activation in vitro and in vivo (Cherwinski et al 2005) as well as efficacy in multiple preclinical models of autoimmune diseases.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.041
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB946 Improvements in acne-prone skin quality correlate with a reduction
           in saliva cortisol levels after use of an 8-week 3-step topical regimen
    • Authors: C. Skobowiat; J.S. Craw, R. Bianchini, K. Fields, K. Rodan, T. Falla
      Abstract: It is well established that both UV exposure and emotional stress can lead to an activation of stress response via the Hypothalamic Pituitary Adrenal (HPA) axis resulting in elevated cortisol levels negatively affecting skin. a, b, cIn this study, we found that subjects using a three-step skincare regimen not only experienced the expected skin benefits but also significantly lower cortisol levels after eight weeks. Thirty women, ages 18-45, applied a three-step skincare regimen featuring antioxidants, electrolytes, prebiotics and a sunscreen to their faces twice a day for eight weeks.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.040
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB945 Using artificial intelligence to understand patient perspectives
           towards treatment of dermatological diseases
    • Authors: J. Jueng; L. Dupuis, A. Su, A. Kunadia, R. Dellavalle, I. Brooks, R. Sinha, B. Maner, F. Siddiqui, V. Burton, J. Seyffert, J. Solomon
      Abstract: Patients with dermatologic conditions frequently use social media, generating millions of posts in which they share about their experience with disease and treatment. Using Artificial Intelligence (AI), we analyzed public social media posts and identified associations between Patient Global Impression of Change (PGIC) and patient sentiments which could provide insight into disease burden. Using the Crimson Hexagon (CH) AI-powered social media database, millions of publicly available social media posts were identified.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.038
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB944 The impact of dermatology consultation for dermatology-related
           admissions in oncology patients
    • Authors: A.Y. Ning; M. Levoska, S. Hill, M. Beveridge
      Abstract: Dermatologic toxicities in oncology patients may result in unnecessary cessation of cancer therapy. Early diagnosis by dermatology can minimize treatment disruption. We aimed to characterize inpatient oncology admissions for dermatologic issues and the impact of dermatology consultation on management. We performed a retrospective chart review of inpatient dermatology consultations from 7/01/2018 to 1/22/2020 at University Hospitals Cleveland Medical Center. Of 794 consultations,138 were for oncology patients.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.037
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB943 Calcipotriol and 5-fluorouracil as neoadjuvant therapy for mohs
           micrographic surgery in treatment of cutaneous squamous cell carcinoma
    • Authors: Y. Humeda; L. Angnardo, E. Corty, L. Hyder, M. Hoffmann, A. Cognetta
      Abstract: Cutaneous squamous cell carcinoma (cSCC) often occurs intermixed with numerous actinic keratoses (AK) within a field of significant actinic damage. When used together, calcipotriol and 5-Fluorouracil (C5FU) synergistically activate a CD4+ T cell-mediated immunity against AK resulting in a mean reduction in AK on the face and scalp when compared to the use of 5FU with Vaseline. We frequently encounter subclinical spread of cSCC in situ beyond the initial biopsied lesion. We investigated the use of C5FU preoperatively in patients with biopsy-proven cSCC compared to Mohs Micrographic surgery (MMS) alone.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.036
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB941 Dupilumab for atopic dermatitis in children aged ≥6 to
    • Authors: A.S. Paller; E.C. Siegfried, L.A. Beck, J.T. O'Malley, B. Shumel
      Abstract: We evaluate dupilumab efficacy and safety by pre-specified baseline weight stratification in children aged ≥6 to
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.034
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB940 Ixekizumab versus guselkumab: 24-week clinical responses and 4-week
           gene expression data
    • Authors: A. Blauvelt; A. Pinter, Y. Tada, H. Elmaraghy, G. Gallo, B.J. Nickoloff, L. Renda, S. Park, L. Guenther
      Abstract: The objective of IXORA-R was to compare the efficacy of the interleukin (IL)-17A antagonist ixekizumab (IXE) and the IL-23p19 inhibitor guselkumab (GUS) over 24 weeks of treatment. Adults with moderate-to-severe plaque psoriasis (sPGA ≥3, PASI ≥12, BSA ≥10%) were randomized to IXE or GUS (1:1; dosing per label). Significantly more patients treated with IXE than GUS had clear skin (PASI 100) from Weeks 4 to 12.1 Here, we report 24-week results for efficacy and safety as well as gene expression data through Week 4.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.033
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB939 Pruritic and dyskeratotic dermatosis: An unusual cutaneous
           manifestation
    • Authors: K.R. Kwan; S. Sears, J. Malone, P. Rady, S. Tyring, S. Bahrami, M. Huelsman, J. Callen
      Abstract: Immunocompromised patients are at increased risk for opportunistic infections. Pruritic and dyskeratotic dermatosis (PDD) is a rare, recently described clinical disease in immunocompromised individuals caused by reactivation of Human Polyomavirus (HPyV) 6 or 7,detectable predominantly in the epidermis. Histologically, specimens lack viropathic effect and may demonstrate “Peacock plumage” characterized by irregular columns of parakeratosis and spotty intraepidermal dyskeratosis. The condition manifests as diffuse, pruritic, gray-brown, lichenified plaques.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.032
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB938 Efficacy of FMX101 4% topical minocycline foam for the treatment of
           moderate-to-severe acne vulgaris: Integrated summary from three phase 3
           studies
    • Authors: J. Del Rosso; L.F. Stein Gold, J. Weiss, L. Green, V. Stakias, I. Stuart
      Abstract: Background: FMX101 4% is a novel FDA-approved minocycline-containing topical foam for the treatment for acne vulgaris. Objective: Compare the efficacy of FMX101 4% vs vehicle foam for acne based on the integrated analysis of 3 Phase 3 studies. Methods: In 3 multicenter, randomized, double-blind, Phase 3 studies (FX2014-04, N=466; FX2014-05, N=495; FX2017-22, N=1488), FMX101 4% was compared to vehicle in subjects with moderate-to-severe acne. Subjects applied the study drug once daily for 12 weeks.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.031
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB937 Post-operative radiation therapy for low-risk Merkel cell carcinoma
           is associated with reduced local recurrences for primary tumors on the
           head/neck, but not other sites
    • Authors: M. Bierma; P. Goff, K. Lachance, D.S. Hippe, S. Schaub, Y. Tseng, S. Apisarnthanarax, J. Liao, P. Nghiem, U. Parvathaneni
      Abstract: Merkel cell carcinoma (MCC) is a rare, aggressive skin cancer with a recurrence risk of ∼40%. Primary treatment typically includes surgery with adjuvant post-operative radiation therapy (PORT) to the primary site to reduce local recurrence (LR) risk. Here, we assess the impact of PORT on LR rates in MCC patients with stage IA head and neck (HN) vs. non-head and neck (N-HN) primary tumors. We conducted a retrospective analysis of 118 low-risk MCC patients treated between 2006 and 2019. Inclusion criteria were study enrollment
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.029
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB936 Tanning bed use and the risk of anxiety and depression
    • Authors: E.X. Wei; H. Nan, X. Li
      Abstract: This study investigates whether early life indoor tanning frequency early in life is associated with risk of incident depression and anxiety later in life. The Nurses’ Health Study II (NHSII) is a prospective cohort study, which started in 1989 with 116,434 US female nurses (ages 25–42 at enrollment). Depression was defined as clinician-diagnosed depression and/or antidepressant use. Anxiety is defined as having an Crown-crisp anxiety scale items greater than or equal to 6 or Generalized Anxiety Disorder score of greater than or equal to 15.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.028
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB935 Impact of prior authorization on patient behavior to fill
           prescription following appointment
    • Authors: A.M. Bashyam; M.B. Gadarowski, A. Ali, W. Haidari, S.D. Pearce, S.R. Feldman, L.C. Strowd
      Abstract: This study evaluated the impact of prior authorization (PA) on patient behavior in filling prescriptions by assessing treatment delay and abandonment. Adult patients prescribed topical tacrolimus for any dermatologic condition in a six-month period were identified. Patients with electronic prescriptions at retail pharmacies were included. Patients whose insurance did not require PA were compared to those who required PA (and were approved). Eligible patients were contacted for a phone questionnaire.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.027
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB934 Outcomes of psoriasis with and without joint involvement
    • Authors: E. Edigin; M.M. Rivera Pavon
      Abstract: Psoriasis is a autoimmune disorder with occasional joint involvement. We analyzed the outcomes of psoriasis with and without joint involvement. The primary outcome was inpatient mortality, while secondary outcomes were hospital length of stay (LOS) and total hospital charge. Data were abstracted from the National Inpatient Sample (NIS) 2016 and 2017 Database. The NIS was searched for psoriasis hospitalizations with and without joint involvement as principal or secondary diagnosis using ICD-10 codes.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.026
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB933 Views and beliefs of people engaged in virtual discussions of
           vitiligo: A qualitative study of messages in online forums
    • Authors: M.B. Gadarowski; A.M. Bashyam, S.R. Feldman
      Abstract: Virtual asynchronous discussion platforms can provide firsthand insight on disease management, psychosocial impact, burden on quality of life, and anecdotal experiences between healthcare providers and the public. We examined the content exchanged by people engaged in online vitiligo forums. An interpretive research paradigm—a means to capture subjective experiences––was utilized to assess public online forum content and the beliefs of individual participants. 39 relevant forums were identified, nine of which met inclusion criteria for a total of 382 anonymous users.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.025
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB932 Reasons for psoriasis hospitalizations with inpatient mortality
    • Authors: E. Edigin; P.O. Eseaton
      Abstract: Psoriasis is a chronic autoimmune disease with an increased risk of medical comorbidities that results in hospitalizations and occasional inpatient mortality. Data were abstracted from the National Inpatient Sample (NIS) Database (the largest collection of inpatient hospitalization data in the United States). The NIS was searched for psoriasis hospitalizations in 2017 with ICD-10 code “L04” as the principal or secondary diagnosis. The principal discharge diagnosis” for psoriasis hospitalizations with in-hospital death was divided into 19 ICD 10 code categories.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.024
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB931 Regional differences in biologic treatment patterns and achievement
           of outcomes within the Corrona Psoriasis Registry across the US
    • Authors: C. Enos; K. O'Connell, R. Harrison, R. McLean, B. Dube, S. Bell, A. Van Voorhees
      Abstract: US treatment targets for psoriasis have been proposed, yet there is little nationwide data on the use and effectiveness of biologics to achieve these targets. This study will determine the geographic variation of biologic treatment patterns and outcomes among adult US patients with psoriasis in the Corrona Psoriasis Registry. Patients were enrolled at 100 sites recruited from 32 states. Analyses included 737 biologic patient-initiations in 2018. Demographics and disease measures were recorded at initiation and 6-months.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.023
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB930 Leaving against medical advice among patients hospitalized for
           dermatologic conditions
    • Authors: R. Tripathi; R.S. Mazmudar, J.S. Bordeaux, J. Scott
      Abstract: Background: Leaving against medical advice (AMA) is associated with worse outcomes for hospitalized patients and disproportionately affects vulnerable patient populations. Despite the growing burden of skin disease in hospitalized patients in the United States, little is known regarding predictors for leaving AMA among patients hospitalized for dermatologic conditions. Objective: To investigate the patient, clinical, and hospital factors associated with leaving AMA in patients hospitalized for dermatologic conditions.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.022
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB929 Determinants of palliative care utilization for metastatic melanoma
    • Authors: R. Tripathi; J.S. Bordeaux, R.I. Nijhawan
      Abstract: Background: Although palliative therapy for metastatic disease is associated with improved quality of life, longer overall survival, and lower end-of-life costs, little is known regarding the utilization of palliative therapy for metastatic melanoma. Our objectives were to investigate factors associated with use of palliative therapy for metastatic melanoma, compare mortality by receipt of palliative therapy, and describe trends in palliative therapy utilization over time. Methods: Cross sectional retrospective study of hospital registry data using the National Cancer Database (2004-2015) of patients with Stage IV cutaneous melanoma (n=21,670).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.021
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB928 Association of indoor tanning frequency during early life with other
           addictive behaviors among US women
    • Authors: H. Tsibris; H. Nan, X. Li
      Abstract: Frequent indoor tanning bed users may be more likely to participate in other addictive behaviors. We evaluated the association of indoor tanning usage with other addictive behaviors including smoking, alcohol use, and caffeine consumption amongst a large prospective cohort of 75,957 female registered nurses using logistic regression analyses adjusted for potential confounding factors. 24.5% of the participants reported indoor tanning. We observed dose-response relationships between the frequency of indoor tanning and the likelihood of past or current smoking (P-trend
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.020
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB927 Predictors of post-operative wound dehiscence: An analysis of the
           northwestern medicine enterprise data warehouse (nmedw)
    • Authors: V. Harikumar; K.A. Reynolds, D. Hsu, B. Chen, J. Lazaroff, E. Gwillim, E. Poon, M. Alam
      Abstract: Background: Dehiscence, a post-surgical complication, is associated with suboptimal patient outcomes and higher costs. Identifying patients at high risk can facilitate interventions aimed towards prevention. Objective: To identify risk factors for dehiscence after skin closure surgery. Methods: We analyzed the Northwestern Medicine Enterprise Data Warehouse (NMEDW), a registry of EHR patient data, to identify cases of dehiscence. “Wound dehiscence” was searched in patient charts from 2014- 2015.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.019
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB926 Hyaluronic acids (HAs) molecular size-dependent biological functions
           on UVB-induced DAMPs-mediated keratinocyte inflammation
    • Authors: L. Hu; Y. Sato, K. Takagi, T. Ishii, Y. Honma, J. Muto
      Abstract: Physiologic responses mediated by hyaluronic acid (HAs) depend on their molecular weights and binding receptors, TLR4 and CD44. Low molecular weight HA (LMW-HA) induces inflammation, while high molecular weight HA (HMW-HA) works as an anti-inflammation factor. UVB-induced epidermal inflammation is usually initiated by endogenous molecules released from damaged cells upon skin tissue injury, called damaged-associated molecular patterns (DAMPs). Calprotectin, a typical DAMP, usually causes severe skin inflammation through activating TLR4 pathways.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.017
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB925 Non-invasive gene-expression analysis of cutaneous T-cell lymphoma
    • Authors: B. Jansen; J.A. Rock, D. Hansen, M. Hanhan, Z. Yao
      Abstract: Purpose: The purpose of this study is to non-invasively identify disease specific genes expressed in skin samples of patients with cutaneous T-cell lymphoma (CTCL). Materials and Methods: Epidermal skin samples were collected non-invasively with adhesive patches from lesional and non-lesional skin of CTCL patients and from non-CTCL skin samples. Gene expression was evaluated on cDNA reversely transcribed from isolated total RNA using magnetic bead extraction and qPCR reactions. Results: Fourteen target genes were assessed across a sample set including 12 CTCL and 11 control samples.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.015
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB924 Presentation of a novel variant of DOCK8: Heterozygous mutation with
           clinical findings
    • Authors: A.S. Weissman; K. Field, S.F. Dinetz, C. Mani, B.B. Wray, K.R. May
      Abstract: Hyper-IgE syndromes (HIES) are characterized by skin abscesses, increased serum IgE, eczema, and recurrent pneumonias. The most common autosomal recessive (AR) HIES are homozygous dedicator of cytokinesis 8 (DOCK8) loss-of-function mutations. These patients develop atopic diseases and cannot control cutaneous infections. While AR homozygous HIES DOCK8-deficiency clinical information has increased, little is known about the impact of DOCK8 heterozygous mutations. We describe a 10y male with a novel heterozygous point mutation in the DOCK8 region at c.624-12 T>A.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.014
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB923 Reduced complexity stratified epidermal equivalent cultures from
           immortalized keratinocytes
    • Authors: B.A. Nanes; G. Danuser
      Abstract: Three-dimensional keratinocyte culture models mimicking stratified squamous epithelium may reveal aspects of skin architecture and function unobservable in traditional two-dimensional monolayer cultures. Such skin equivalent cultures are well-established and were among the first organotypic culture systems developed. However, traditional skin equivalent cultures are relatively complex, requiring primary keratinocytes with limited replication potential and relying on a fibroblast and collagen dermis-like layer to stimulate keratinocyte differentiation.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.012
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB922 The evaluation of moisturizing products on in vitro dry-skin
           mimicking 3D model
    • Authors: S. Hong; A. Nguyen, R. Metha, K. Kadoya
      Abstract: Dry skin is a characteristic appearance of impaired skin barrier function. Since dry skin is due to excessive water loss, topical application of moisturizing products has been a main remedy to keep more moisture in skin and to ameliorate adverse symptoms. However, the effect of active ingredients on the biological structure of dry skin has not been widely tested due to a lack of methodology to properly evaluate them systematically. Previously (LB1083, SID meeting 2019), we demonstrated Tracer assay (barrier function assay), focusing on the effect of moisturizers to address water loss on the 3D cell culture model (EpiDerm, MatTek).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.011
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB921 Relationship between functional group interaction of lipid
           components in stratum corneum and barrier function of skin
    • Authors: Y. Obata; Y. Ofuchu, H. Setoyama, K. Ishida
      Abstract: The stratum corneum located at the outermost layer of the skin plays a physical protective function for living body from exogenous hazards. Numerous studies have shown that disruptions in the organization of stratum corneum lipids may be associated with decreased skin barrier function. In this study, we used CER [NDS] and CER [NP] as ceramides (CER), cholesterol, and palmitic acid (PA) to understand the barrier function formed by the organization of lipids. Lipid models containing typical subclass CER prepared, its nanostructure and characteristics was examined by differential scanning calorimetry, synchrotron X-ray diffraction and infrared spectroscopy.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.010
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB920 IQGAP3 is an important mediator of the psoriatic phenotype of
           keratinocytes
    • Authors: A. Zolotarenko; A. Zavarukhina, S. Bruskin
      Abstract: Keratinocytes are immunocompetent cells important for the structural and barrier function of skin. Psoriatic keratinocytes are characterized by the enhanced proliferation and reduced differentiation rates as well as by the elevated production of proinflammatory cytokines and chemoattractants. In this research we have knocked-down a scaffold protein IQGAP3 using shRNA in HaCaT keratinocytes (HaCaT_shIQ3) in order to test the hypothesis if IQGAP3 mediates the psoriatic phenotype of keratinocytes. IQGAP interacts with cell adhesion molecules, with the cytoskeleton, with signaling molecules to regulate cell morphology, motility and kinase pathways.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.009
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB919 Aging does not facilitate nodular BCC development in mice expressing
           oncogenic Smo
    • Authors: M. Grachtchouk; D. Wilbert, A. Hoover, D. Pyrozhenko, M. Verhaegen, A.A. Dlugosz
      Abstract: Aberrant Hedgehog (Hh) signaling drives the development of basal cell carcinomas (BCCs), which arise mainly in elderly patients. Loss-of-function PTCH1 or gain-of-function SMO mutations are oncogenic drivers in human BCC, but neither of these genetic alterations effectively yield nodular BCCs in genetically-engineered mice. Because nearly all prior BCC modeling studies have been performed in juvenile mice, we set out to assess whether aging is permissive for BCC development using a Cre-inducible oncogenic SmoM2 allele, activated in skin of young versus aged mice.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.007
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB918 Tirbanibulin, a novel inhibitor of tubulin polymerisation and src
           kinase signaling, for actinic keratosis (AK): Results of two phase-3
           studies and 1-year follow-up data
    • Authors: A. Blauvelt; S. Kempers, S. Puig, G. Martin, E. Fumero, A. Grada, D. Cutler, J. Fang
      Abstract: Objectives: Two pivotal Phase III randomized, double-blinded, vehicle-controlled, studies (KX01-AK-003/KX01-AK-004) evaluated the efficacy and safety of tirbanibulin ointment 1% vs. vehicle in adults with AK on the face/scalp. Methods: Eligible subjects with 4-8 clinically visible AK lesions in a 25 cm2 area were randomized 1:1 to receive tirbanibulin or vehicle (5-day once-daily self-application). Primary endpoint was complete clearance of AK at Day 57. Safety assessments included local skin reactions (LSRs) and adverse events.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.006
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB917 Platelet-activating factor-receptor signaling mediates targeted
           therapy-induced microvesicle particle release in lung cancer cells
    • Authors: S.J. Chauhan; A. Thyagarajan, Y. Chen, R.P. Sahu
      Abstract: Microvesicle particles (MVP) are nano-sized extracellular vesicles, secreted in response to stimuli such as therapeutic agents by a variety of cell types including tumor cells. MVP are involved in mediating several cellular responses including modifying tumorigenicity or sensitivity of therapeutic agents. Studies, including ours, have shown that tumor cells expressing a G-protein coupled, platelet-activating factor-receptor (PAF-R) augments pro-oxidative stressors including chemotherapy-mediated MVP release.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.005
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB916 Effects of the platelet-activating factor-receptor and microRNA-149
           in lung cancer growth and therapy effectiveness
    • Authors: S.J. Chauhan; A. Thyagarajan, R.P. Sahu
      Abstract: Lung cancer remains the leading cause of cancer-related deaths, with low response rates to the current treatment options, indicating the need to explore potential factors, involved in lung cancer growth or impeding the efficacy of therapeutic agents. Studies, including ours, have shown the critical roles of a G-protein coupled, platelet-activating factor-receptor (PAF-R) signaling in augmenting tumor growth or limiting therapy effectiveness in various experimental cancer models. While several mechanisms of the PAF-R pathway have been proposed, its effect with microRNAs (miRs) has not been studied.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.004
      Issue No: Vol. 140, No. 7 (2020)
       
  • LB915 A targeted CRISPR screen to identify essential genes for the TRM T
           cell response to cancer
    • Authors: J.B. Williams; Y. Contreras Duarte, E. Rotrosen, T.S. Kupper
      Abstract: Bunkered in barrier tissue, tissue-resident memory T (TRM) cells are strategically positioned to react quickly to tissue perturbations, such as infection, injury, or cancer. Recent studies have highlighted a potential role for TRM cells as a targetable T cell population due to their abundance in most tissues. To better understand the role TRM cells play in the antitumor immune response, we developed a targeted CRISPR screen to identify the essential genes regulating the TRM-response to tumors. We first developed a murine tumor model system in which OT-I cells could be adoptively transferred and re-isolated from tumors.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.05.002
      Issue No: Vol. 140, No. 7 (2020)
       
  • Subscription Information
    • Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/S0022-202X(20)31214-8
      Issue No: Vol. 140, No. 7 (2020)
       
  • 913 A variant of Mohs micrographic surgery: The muffin technique
    • Authors: P. Surmanowicz; A. Sivanand, A. Du, R. Gniadecki
      Abstract: Background: Moh’s micrographic surgery (MMS) is the gold standard treatment for non-melanoma skin cancers (NMSC) such as basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). Despite the advantages of MMS, the technique also has limitations. The procedure can be time-consuming and, due to the bulky nature of many cutaneous tumors, appropriately sectioning and flattening the specimen for cryosection without omitting tissue is difficult. A novel method has recently been proposed as an alternative to the traditional MMS approach.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.929
      Issue No: Vol. 140, No. 7 (2020)
       
  • 911 Lebrikizumab attenuates the neuronal enhancement of pruritus through
           IL-13 consistent with clinical anti-itch effects observed in phase 2b
           moderate-to-severe atopic dermatitis patients
    • Authors: E. Simpson; A.S. Paller, F. Cevikbas
      Abstract: Recent studies have provided a better understanding of cytokines playing a role in pruritus (itch), a cardinal feature in atopic dermatitis (AD). Among these cytokines, IL-13 is a key mediator of multiple pro-inflammatory processes in AD. Other type-2 cytokines, such as TSLP, IL-33, and IL-4 modulate neurons directly, in addition to their pro-inflammatory effects. Lebrikizumab, a novel, high-affinity, monoclonal antibody targeting IL-13, is under investigation for the treatment of moderate-to-severe AD.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.927
      Issue No: Vol. 140, No. 7 (2020)
       
  • 910 Increased eosinophils as a biomarker for therapeutic response in
           patients with chronic pruritus of unknown origin
    • Authors: R. Khanna; S.P. Patel, K. Williams, S. Kwatra
      Abstract: Chronic pruritus of unknown origin (CPUO) is pruritus lasting greater than 6 weeks that is not linked to a causal disease process. With a lack of approved therapies, CPUO is extremely difficult to manage. We thus conducted a retrospective review of 23 patients collecting demographics, medical history, laboratory data including complete blood count with differential, pruritus characteristics, and response to treatment. Of these patients, those with an absolute eosinophil count above 0.30 K/cu mm, eosinophil percent above 4%, or pronounced eosinophils on skin biopsy were identified.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.926
      Issue No: Vol. 140, No. 7 (2020)
       
  • 906 Early effector T-cell densities predict response in patients with
           advanced Merkel cell carcinoma treated with anti-PD-1
    • Authors: S. Lu; F. Succaria, B. Green, N. Giraldo-Castillo, J. Taube
      Abstract: Background: We previously nominated PD-1+ cell densities in pre-treatment tumor specimens as a potential biomarker for response to pembrolizumab (anti-PD-1) in patients with advanced Merkel cell carcinoma (MCC). Here, we used multiplex immunofluorescence (mIF) in a separate cohort of MCC patients treated with anti-PD-1 to determine which immune cell subset(s) contribute PD-1 to the tumor microenvironment (TME). We also characterized PD-1 expression intensity on lymphocytes, as an indication of their functional state, and tested for an association with anti-PD-1 response.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.922
      Issue No: Vol. 140, No. 7 (2020)
       
  • 905 Sex differences in the molecular cause of perioral skin wrinkling
    • Authors: I. Brown; C. Dillen, B.C. Ly, N. Shyam, S. Kang, A. Chien
      Abstract: Wrinkling is the hallmark of skin aging. We have previously reported that perioral wrinkling is more severe in females; however the molecular basis for this is unknown. We enrolled 12 subjects (n=6 male/female) age 54-86 with Griffith’s photoaging severity grade between 4 and 8 (0=none, 8=severe) and took biopsies from both the perioral and periocular region. Using qPCR, we assessed RNA expression levels of collagen I, collagen III, cysteine-rich angiogenic inducer 61 (CYR61), and insulin like growth factor 1 (IGF-1).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.921
      Issue No: Vol. 140, No. 7 (2020)
       
  • 904 Fluorescence Imitating Brightfield Imaging (FIBI): A novel application
           of rapid, non-destructive and slide-free skin tissue imaging
    • Authors: T. Engel; A. Rangchi, M. Fung, M. Kiuru, F. Fereidouni, R. Levenson
      Abstract: Purpose: To investigate the functionality of Fluorescence Imitating Brightfield Imaging (FIBI) for imaging skin tissue samples. Methods: The surfaces of three (with more to follow) skin samples from the UC Davis Dermatopathology tissue bank were deparaffinized using xylene and ethanol washes, then stained with hematoxylin, Scott’s bluing reagent and eosin. A color camera captured images from 405 nm visible range (blue) excitation light illuminating the specimens. Standard hematoxylin and eosin (H&E) slides were cut from specimens prior to deparaffinization and compared to FIBI images.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.920
      Issue No: Vol. 140, No. 7 (2020)
       
  • 903 The IgG1 isotype of anti-MDA5 antibody may dominate severity of
           interstitial lung disease in dermatomyositis
    • Authors: M. Chen; J. Zheng, M. Pan, H. Cao
      Abstract: Objective To identify anti-MDA5 antibody subtype (IgG, IgA, IgM) and anti-MDA5 IgG subclasses, and to investigate their association with clinical severity. Methods Clinical features, laboratory findings and serum of 36 DM/CADM patients with anti-MDA5 antibody positive were collected and analyzed. Anti-MDA5 IgG was measured by enzyme-linked immunosorbent assay and line-blot immunoassay. Anti-MDA5 subtype (IgG, IgA, IgM) and anti-MDA5 IgG subclasses were measured by enzyme-linked immunosorbent assay.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.919
      Issue No: Vol. 140, No. 7 (2020)
       
  • 902 Assessing performance of deep neural networks used for image
           classification by stress testing
    • Authors: A.T. Young; J. Pfau, M.J. Keiser, M. Wei
      Abstract: Deep convolutional neural networks (CNNs) can outperform dermatologists on image classification. However, their performance in a real-world setting, where image capture is subject to variations in lighting, zoom, focus, etc. is not well studied. Recent work in the machine learning community has reported that CNNs predictions are not stable to perturbations of the input image, e.g. a simple rotation. Standardized metrics of model robustness, to be reported in addition to accuracy, are needed to assess the readiness of dermatologist-level CNNs for clinical use.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.918
      Issue No: Vol. 140, No. 7 (2020)
       
  • 901 Comparison of changes in serum metabolites before and after treatment
           with IL-17A monoclonal antibody and halomethasone in psoriasis
    • Authors: Y. Le; L. Zhang, L. Chen, X. Li, Y. Yu, J. Zheng
      Abstract: OBJECTIVE: To analyze the changes of serum metabolites of patients with psoriasis after IL-17A monoclonal antibody (mAb) treatment and halomethasone treatment, and to compare the effects of the two treatment methods on patients' systemic metabolism. METHODS: Metabolomics techniques based on gas chromatography-mass spectrometry were used to analyze the serum metabolites before and after the treatments in 32 patients treated with IL-17A mAb and 16 patients treated with halomethasone. RESULTS: 19 serum differential metabolites were screened out before and after IL-17A mAb treatment, while 9 serum differential metabolites were screened out after treatment with halameasone.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.917
      Issue No: Vol. 140, No. 7 (2020)
       
  • 900 Utilization of an oxygen-sensing bandage to distinguish cellulitis
           from clinical mimickers
    • Authors: C. Gabel; H. Marks, E. Roussakis, A. Raff, C. Thomas, E. Nguyen, R. Shah, C. Evans, D. Kroshinsky
      Abstract: Cellulitis is a cutaneous infection of the dermis and subcutaneous tissue. There are no existing gold standard diagnostic techniques for differentiating between cellulitis and clinical mimickers ("pseudocellulitis"). New optical technologies in development could aid diagnosis, specifically: an oxygen-sensing bandage developed by this group. There is evidence that inflammation, metabolic derangement and vascular changes alter tissue oxygenation (pO2). Detection of these changes may help differentiate infectious vs.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.916
      Issue No: Vol. 140, No. 7 (2020)
       
  • 898 Endotype analysis in mucous membrane pemphigoid
    • Authors: K. Messingham; T. Crowe, M. Greiner, J. Hellstein, D. VanDaele, S. Palmer, J. Fairley
      Abstract: Mucous membrane pemphigoid (MMP) is an autoimmune blistering disease involving mucosae with heterogeneous disease presentation and autoantigen reactivity. The goal of this study was to determine if MMP could be resolved into endotypes, based on clinical features or antigenic target. 71 patients (37 female/ 34 male) who met clinical and histologic criteria for MMP were identified in our REDCap database; clinical information and sites of involvement were confirmed by exam and chart review. Serum autoantibody targets were evaluated via indirect immunofluorescence, ELISA (BP180, BP230, Col VII), and blotting (BP180, α6β4 integrin and Laminin 332).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.914
      Issue No: Vol. 140, No. 7 (2020)
       
  • 897 Association of urine mono-benzyl phthalate levels with increased
           psoriasis severity
    • Authors: S.P. Patel; J. Choi, R. Khanna, K. Williams, Y. Semenov, S. Kwatra
      Abstract: Plastic products produced with phthalates as plasticizers have created health concerns since phthalates are believed to cause hormonal and immune dysregulation. Thus, we sought to explore the association between phthalate exposure and psoriasis. Using the 2005-2006 National Health and Nutrition Examination Survey, we conducted a cross-sectional analysis to investigate the association between urinary phthalate metabolite levels and psoriasis severity. We calculated odds ratios (OR) using logistic regression and performed a linear regression to assess for a relationship between urine phthalate levels and psoriasis severity.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.913
      Issue No: Vol. 140, No. 7 (2020)
       
  • 896 Concordance of teledermatology assessment with in-person,
           histopathological and laboratory results
    • Authors: A. Tam; N. Bhari, S. Khandpur, M. Ramam, M. Wei
      Abstract: Almost 90% of the world’s population has mobile coverage; recent advances in mobile technology hold promise to improve access to dermatology care worldwide. However, research on the use of mobile technology for teledermatology in underserved regions remains limited. We conducted a pilot study to compare diagnostic concordance of assessment of images vs. in-person assessment, histopathological and/or laboratory results in an outpatient dermatology clinic at an academic institution in India. Of the 152 patient cases, 127 cases had both in-person assessment and diagnosis rendered by photo 1-2 months later, by the same dermatologist, with an intra-observer concordance of 91.3%.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.912
      Issue No: Vol. 140, No. 7 (2020)
       
  • 895 Oral glucoraphanin and curcumin supplements induce the key
           cytoprotective enzyme NAD(P)H dehydrogenase [quinone] 1 (NQO1) in the skin
           of healthy human subjects
    • Authors: A. Chien; S. Rachidi, R. Wang, K. Wade, H. Liu, J. Fahey, S. Kang
      Abstract: Oxidative stress plays a pivotal role in skin aging and carcinogenesis. NAD(P)H dehydrogenase [quinone] 1 (NQO1; EC:1.6.5.2) is a prototypical phase 2 enzyme involved in cytoprotection from oxidative stress and mutagenicity. Phytochemicals such as sulforaphane (SF) or curcumin (CUR) can be highly protective by inducing these enzymes in mammals. Topical SF or its precursor glucoraphanin (GR) from broccoli sprouts induce NQO1 activity in mice. Ex vivo treatment of full thickness human skin with SF induces NQO1 gene expression.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.911
      Issue No: Vol. 140, No. 7 (2020)
       
  • 894 Molecular analysis of primary melanoma T cells identifies patients at
           risk for metastatic recurrence
    • Authors: W. Pruessmann; J. Rytlewski, J. Wilmott, M.C. Mihm, G.H. Attrill, B. Dyring-Andersen, P. Fields, Q. Zhan, A.J. Colebatch, P.M. Ferguson, J.F. Thompson, K. Kallenbach, E. Yusko, R.A. Clark, H. Robins, R.A. Scolyer, T.S. Kupper
      Abstract: Primary melanomas>1 mm thickness can be cured by resection, but may recur metastatically. We assessed the prognostic value of “T cell fraction” (TCFr) and “repertoire T cell clonality”, as measured by high-throughput sequencing of the T cell receptor beta-chain (TCRB), in primary melanomas (n=377) from patients followed five years. We included T2-T4 melanomas. All samples were scored for TIL content by histopathology. To assess T cell subsets, we further stained sections by multiplex immunohistochemistry (mIHC) for CD3, CD8, CD4, FoxP3, CD39 and CD103.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.910
      Issue No: Vol. 140, No. 7 (2020)
       
  • 893 Next-generation sequencing and computational modeling identifies the
           genomic signature of isotretinoin in acne patients
    • Authors: Z. Nolan; A. Schneider, Z. Cong, A. Longenecker, D. Thiboutot, A. Nelson
      Abstract: Despite nearly 40 years of clinical use and research, the mechanism of action of isotretinoin in the treatment of severe acne is still relatively unknown. Utilizing next-generation sequencing and computational modeling, we investigated the genomic changes induced by isotretinoin treatment in both cell lines and in patient skin samples. Sebocytes (SEB-1), keratinocytes (NHEK), and fibroblasts (NHDF) were treated with either vehicle or 0.1μM isotretinoin for 72hrs. Skin punch biopsies were collected from non-lesional back skin of acne patients prior to isotretinoin treatment, after 1, 8, or 20 weeks of treatment, and 6 months after cessation of therapy (n> 6/time point).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.909
      Issue No: Vol. 140, No. 7 (2020)
       
  • 892 Differentially expressed plasma proteins in pityriasis rubra pilaris
           patients treated with ixekizumab
    • Authors: J.L. Strunck; D. Haynes, C. Topham, A. Ortega-Loayza, T.M. Greiling
      Abstract: Pityriasis Rubra Pilaris (PRP) is a rare and debilitating cutaneous disease characterized by widespread red scaly plaques, follicular papules, and palmoplantar keratoderma. The pathogenesis of PRP is poorly understood, although overexpression of Th17 cytokines have been reported suggesting an inflammatory pathogenesis that may share features with psoriasis. In this study, we used OLINK proximity extension assay technology to quantitate 92 plasma inflammatory proteins of 11 PRP patients treated with ixekizumab (NCT03485976).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.908
      Issue No: Vol. 140, No. 7 (2020)
       
  • 890 Buddy relationships in dermatologic excisions: A novel one-to-one peer
           mentorship program for patients with skin cancer
    • Authors: S. Zullo; C. Miller, T. Shin, J. Etkzorn, J. Sobanko
      Abstract: Facial skin cancers impair psychosocial health. Peer mentorship programs provide emotional and social support for many oncologic patients but have not been studied in skin cancer. This pilot study evaluated feasibility and satisfaction with a peer mentorship program for facial skin cancer patients treated with Mohs micrographic surgery (MMS). An IRB-approved, randomized controlled trial was performed in an academic center from 2018 to 2019. Patients one year removed from MMS treatment of a facial skin cancer were enrolled and trained as mentors.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.906
      Issue No: Vol. 140, No. 7 (2020)
       
  • 889 Interleukin-9 promotes malignant T cell survival by inhibiting
           oxidative stress and lactic acidosis in cutaneous T cell lymphoma
    • Authors: S. Kumar; B. Dhamija, S. Marathe, A. Karulkar, N. Sharma, H. Jain, R. Purwar
      Abstract: Cutaneous T cell lymphoma (CTCL) represents a distinct subgroup of non-Hodgkin lymphomas of mature skin tropic malignant T cells. Although the immune dysregulation is critical for the pathogenesis of lymphoma, the precise mechanism of CTCL pathogenesis remains unknown. Recently, few studies reported the defective T cell function during the onset and progression of certain types of lymphoma. However, the presence of interleukin-9 (IL-9) producing Th9 cells and more importantly, the roles of IL-9/Th9 axis in tumor cell metabolism and survival remain unexplored.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.905
      Issue No: Vol. 140, No. 7 (2020)
       
  • 888 Rapid capture, extraction, and analysis of sweat samples for
           quantification of inflammation biomarkers using a novel wearable
           microfluidic system
    • Authors: M. Zhang; A. Aranyosi, S. Lee, J. Model, A. Leech, W. Li, S. Chen, J. Rogers, A.S. Paller, R. Ghaffari, S. Xu
      Abstract: Eccrine sweat is a rich, accessible biofluid comprised of a mixture of electrolytes, metabolites, hormones, and cytokines. Collection of sweat typically relies on absorbent pads that are incompatible with remote monitoring and deployment in field settings due to contaminants in the pads, long-term exposure of sweat to skin, and the need for centrifuges for extraction. Recent advances in the development of wearable microfluidic sensors that capture and analyze sweat dynamics offer new possibilities for tracking sweat biomarkers.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.904
      Issue No: Vol. 140, No. 7 (2020)
       
  • 887 Metabolomics: Unlocking the blueprint of skin aging in Caucasian and
           Chinese women
    • Authors: K. Corallo; E. Pelle, M. Morse, N. Pernodet
      Abstract: For more than a decade, we have demonstrated the importance of circadian rhythm for skin homeostasis and repair. To advance this research, our objective was to identify metabolite profile changes in skin over a 12-hour period, and to understand how age and treatment influence these changes in both Caucasian and Chinese women. An emerging technique for skin diagnostics is metabolomics, or the study of metabolites, which are small molecule substrates, intermediates, and end-products of biological processes.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.903
      Issue No: Vol. 140, No. 7 (2020)
       
  • 886 Emulating IL-17 – CCL20 axis to identify surrogate markers of
           psoriasis using 3D psoriasis tissue model
    • Authors: S. Ayehunie; T. Landry, Z. Stevens, A. Armento, M. Klausner
      Abstract: Psoriasis is a chronic, immune mediated inflammatory skin disease marked by hyperproliferation, abnormal keratinocyte (KC) differentiation, and leukocyte infiltration. Interleukin (IL)-17 – CCL20 axis has been identified as a critical pathway In the Pathogenesis of psoriasis. Overexpression of IL-17A has been implicated with epidermal hyperproliferation and a robust inflammatory response. Since T cells, particularly Th17 cells (IL-17 producing cells), are implicated in inflammatory skin diseases and because targeting IL-17 has been a promising approach in clearing moderate to severe plaque psoriasis, we investigated the role of T cell cytokines such as IL-17 alone or in combination with interferon (IFN)-γ (Th1 cytokines) in exacerbating inflammatory responses using a reconstructed 3D human psoriatic tissue model.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.902
      Issue No: Vol. 140, No. 7 (2020)
       
  • 885 Major producers of IFNg and CB2 receptor distribution in
           dermatomyositis
    • Authors: S. Maddukuri; J. Patel, C. Bax, M. Wysocka, V. Werth
      Abstract: Dermatomyositis (DM) is an autoimmune disease that affects skin and muscle. Type I IFNs, such as IFNb, are implicated in the pathogenesis of DM, but little is known about IFNg. Our prior studies showed a reduction of IFNg after treatment with Lenabasum, a nonpsychoactive CB2 receptor (CB2R) agonist. We aim to identify major producers of IFNg and understand the distribution of CB2R in the blood vs skin of DM patients. Flow cytometry was performed on PBMCs from 3 healthy controls (HC) and 7 DM patients.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.901
      Issue No: Vol. 140, No. 7 (2020)
       
  • 884 Treatment of Netherton syndrome patients with Ixekizumab: A case
           series
    • Authors: C. Barbieux; M. Bonnet des Claustres, M. Bagot, E. Bourrat, A. Hovnanian
      Abstract: Netherton Syndrome (NS) is a rare autosomal recessive skin disease due to loss-of-function mutations in SPINK5 encoding LEKTI. NS combines a severe skin barrier defect with superficial scaling, skin inflammation, and multiple allergies associated with high serum IgE levels. Increased IL-17 pathway has been reported in NS patients’ skin and peripheral blood, identifying IL-17 as a potential therapeutic target. We report compassionate treatment of 3 adult NS patients with the humanized anti-IL-17 monoclonal antibody ixekizumab (IXE).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.900
      Issue No: Vol. 140, No. 7 (2020)
       
  • 883 ‘DermAnnotation’ is All You Need; methodology to transfer
           knowledges of dermatologists to artificial intelligence
    • Authors: K. Shido; E. Kondo, K. Kojima, K. Yamasaki, S. Aiba
      Abstract: Potential of artificial intelligence (AI) for image analysis is proved year by year in many fields including medical situation. Since most of skin diseases reveal their symptoms on skin surface, AI application to dermatology clinic is highly expected. However, even though lesions are on skin, anonymous image collection of skin diseases cannot develop accurate dermatology AI or lead misdiagnostic AI to make confusion in dermatology in worst scenarios, because dermatological diagnosis is a sort of state-of-the-art inspection technique.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.899
      Issue No: Vol. 140, No. 7 (2020)
       
  • 882 Machine learning for measuring scratching in atopic dermatitis using a
           skin-mounted, soft and wireless sensor: Model selection, feature
           extraction, and training set performance
    • Authors: K. Chun; J. Kim, H. Jo, J. Lee, R. Lee, M. Zhang, K. Lee, J. Chang, A. Fishbein, A.S. Paller, J. Rogers, S. Xu
      Abstract: Pruritus is a hallmark of atopic dermatitis (AD). Measuring scratching activity, a natural response to itch, is one potential surrogate marker for assessing pruritus. However, current methods such as actigraphy to objectively quantify pruritus, particularly in children, are limited due to confounding from non-scratching hand motions and omitting fine finger scratching. In order to more accurately measure scratching, we present the use of a small, skin-mounted soft flexible sensor (4.5 cm x 2.1 cm, 7 g) with an embedded high frequency 3-axis (0-2000 Hz) accelerometer in direct mechanical communication with the dorsum of the hand to simultaneously capture motion and acoustic signatures of scratching.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.898
      Issue No: Vol. 140, No. 7 (2020)
       
  • 881 Antisynthetase syndrome and dermatomyositis immunophenotyping
    • Authors: J. Patel; A. Ravishankar, S. Maddukuri, M. Bashir, C. Bax, V. Werth
      Abstract: Antisynthetase syndrome (AS) and Dermatomyositis (DM) are rare inflammatory autoimmune diseases that some consider as separate entities. AS is poorly defined and some patients have skin lesions consistent with DM. To investigate differences between AS and DM relative to control (HC), we identified immunophenotypes. 3 AS, 3 DM, and 3 HC formalin-fixed, paraffin-embedded (FFPE) samples obtained from back, arm, or leg were stained via immunofluorescence for MxA and IFNβ. Three 20x regions were acquired on Nikon Eclipse.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.897
      Issue No: Vol. 140, No. 7 (2020)
       
  • 880 Targeted IL-17RA antagonism ameliorates histological and
           transcriptomic features of hidradenitis suppurativa: A proof of concept
           study
    • Authors: J.W. Frew; K. Navrazhina, S. Garcet, J. Krueger
      Abstract: Hidradenitis Suppurativa is an autoinflammatory disorder of keratinization with a major inflammatory contribution from the Th17 axis. We have previously shown that in addition to previously described IL-17A & IL-17F; IL-17C is found at higher levels by both IHC and rt-PCR in lesional HS skin. We asked whether the IL-17 axis mediates disease activity by using an IL-17RA antagonist Brodalumab to examine histological and transcriptomic features of disease in 10 individuals with HS. Biopsies as per previously published consensus criteria were taken at Baseline and at Weeks 4 and 12 after treatment with an IL-17RA antagonist.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.896
      Issue No: Vol. 140, No. 7 (2020)
       
  • 879 Novel injectable coolant for reducing cutaneous nerve fiber density
    • Authors: S. Moradi Tuchayi; Y. Wang, I. Pence, C. Evans, R. Anderson, L. Garibyan
      Abstract: Postherpetic neuralgia (PHN) is a dermatologic disorder with no established cure. The distinct dermatomal distribution of PHN makes the peripheral nerve supplying the dermatome and its distal cutaneous branches the ideal therapeutic target; however, the short acting nature of current medications limits the use of this localized approach. Herein we demonstrate the effects of a drug-free, injectable coolant with long-lasting pain suppression capabilities that could potentially be used for treatment of PHN.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.895
      Issue No: Vol. 140, No. 7 (2020)
       
  • 878 Investigating T cell phenotype and function in delayed-type drug
           hypersensitivity reactions
    • Authors: G. Romar; P. Hsieh, M. Liang, B. Schmidt, R. Foreman, S.J. Divito
      Abstract: Delayed-type drug hypersensitivity reactions (DHR) range in severity from mild rash to severe sloughing of skin and mucosal surfaces, with or without internal organ involvement. Pathobiology is poorly understood including the phenotype and function of T cells mediating DHR. We performed transcript analysis of 186 genes using Nanostring on FFPE skin samples from adult and pediatric patients with morbilliform drug eruption (MDE) (n=7), Drug Rash with Eosinophilia and Systemic Symptoms (DRESS) (n=6), and Stevens-Johnson Syndrome/Toxic Epidermal Necrolysis (SJS/TEN) (n=14).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.894
      Issue No: Vol. 140, No. 7 (2020)
       
  • 877 Quality of life among family members of patients with atopic
           dermatitis and psoriasis
    • Authors: V. Taliercio; A. Snyder, L. Webber, A. Langner, B. Rich, A. Beshay, D. Ose, R. Hess, J. Rhoads, A.M. Secrest
      Abstract: Chronic inflammatory skin diseases like psoriasis (PsO) and atopic dermatitis (AD) severely impact the quality of life (QoL) of patients. However, the effect of these diseases can extend beyond the affected patients and diminish their families’ QoL as well. We held focus groups and interviews with 23 family members–most commonly significant others–twelve had a family member with AD and 11 had a family member with psoriasis to understand these impacts. After two researchers independently coded the transcripts, we conducted a thematic analysis using a grounded-theory approach to arrive at a consensus on the major themes.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.893
      Issue No: Vol. 140, No. 7 (2020)
       
  • 876 When itchiness is not enough: Understanding the impact of pain on
           experiences with atopic dermatitis
    • Authors: A. Snyder; V. Taliercio, L. Webber, A. Langner, B. Rich, A. Beshay, D. Ose, R. Hess, J. Rhoads, A.M. Secrest
      Abstract: Pain, separate from pruritus, is a relatively unappreciated, and poorly understood, symptom of atopic dermatitis (AD) that can affect patients’ health-related quality of life (HRQoL). This qualitative study involved 24 patients with AD and 12 family members who participated in focus groups and interviews aimed at understanding the impact of pain on AD patients and their family members to capture how this symptom affects HRQoL. Thematic analysis was conducted using grounded theory approach. Two researchers independently coded the transcripts and reached a consensus on major themes.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.892
      Issue No: Vol. 140, No. 7 (2020)
       
  • 875 Insights into the far-reaching effects of the itchiness of atopic
           dermatitis
    • Authors: V. Taliercio; A. Snyder, L. Webber, A. Langner, B. Rich, A. Beshay, D. Ose, R. Hess, J. Rhoads, A.M. Secrest
      Abstract: Atopic dermatitis (AD) is a chronic inflammatory skin condition characterized by xerotic and eczematous lesions. Severe itchiness is the cardinal symptom of this disease and significantly impacts individuals' health-related quality of life. This study aimed to qualitatively explore the experiences of participants with AD and their family members who live with the itchiness of AD. Twenty-four participants with AD and 12 of their family members participated in focus groups and interviews. After two researchers independently coded the narratives, we conducted a thematic analysis using a grounded theory approach to arrive at a consensus on the major themes.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.891
      Issue No: Vol. 140, No. 7 (2020)
       
  • 874 Understanding the triggers and consequences of atopic dermatitis
    • Authors: A. Snyder; V. Taliercio, L. Webber, A. Langner, B. Rich, A. Beshay, D. Ose, R. Hess, J. Rhoads, A.M. Secrest
      Abstract: Atopic dermatitis (AD) is a common skin condition that can affect many aspects of a patient’s health-related quality of life (HRQoL). However, during the time-constrained clinical visit, clinicians rarely capture a complete picture of the impact of AD on the daily home lives of our patients. This study aims to understand triggers and consequences of AD to help clinicians better grasp the full implications of living with AD on an individual and their family members. For this qualitative study, 24 patients with AD and 12 family members participated in focus groups and interviews with questions on how AD affects all aspects of their lives and relationships.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.890
      Issue No: Vol. 140, No. 7 (2020)
       
  • 873 A computational method to automate acne lesion counting
    • Authors: E. Bernardis
      Abstract: Counting primary acne lesions such as comedones, pustules/papules, or nodules is a first necessary step to measuring acne severity both for patient monitoring and for clinical trials. While clinicians quickly estimate the number of lesions upon a visual inspection, a precise count of all lesions present can be time consuming, and, additionally, for clinical trials, many hours of training are often needed to decrease inter-rater variability. The task becomes even more difficult when working with photographs, as in addition to the multifaceted appearance of acne lesions, challenges are posed by varying illumination and skin color.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.889
      Issue No: Vol. 140, No. 7 (2020)
       
  • 872 IL-1β is a potential central mediator to Papulopustular Rosacea
           pathology as determined by paired transcriptomic and proteomic analysis
    • Authors: J. Harden; Y. Shih, D. Rajendran, J. Xu, R. Li, H. Hofland, A.L. Chang
      Abstract: Papulopustular rosacea (PPR) is a chronic inflammatory skin disease characterized by redness, sensitive skin, and inflammatory lesions. Multiple inflammatory pathways have been described to be upregulated in PPR; however, a complete mechanistic understanding of the central mediators of PPR lesions requires further study. To this end, we quantitatively evaluated both the transcriptomic (RNAseq) and proteomic (OLINK® and MSD®) signature of paired non-lesional (NLS) and lesional (LS) PPR biopsy explants (n=5 patients).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.888
      Issue No: Vol. 140, No. 7 (2020)
       
  • 871 Continuous hair density differentiation to score extent of alopecia
           areas automatically
    • Authors: T. Mane; A. Bayramova, L. Castelo-Soccio, E. Bernardis
      Abstract: Commonly used alopecia severity scales usually rely on having clinicians individuate areas of hair loss and compare their locations and/or sizes in order to map the hair loss appearance to a score, such as overall percent alopecia (e.g., Severity of Alopecia Tool, SALT, score) or hair loss top extent score (e.g., Central Scalp Alopecia Photographic, CSAP, scale). To automate this process, we developed a computational tool that takes standardized photographs of patients’ scalps and returns an overall percent hair loss as well as a top extent alopecia shape score as output without any user input.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.887
      Issue No: Vol. 140, No. 7 (2020)
       
  • 870 Clinical and histopathologic characteristics of metastatic and locally
           aggressive basal cell carcinomas
    • Authors: L.X. Baker; E. Grilletta, J.P. Zwerner, A.S. Boyd, L. Wheless
      Abstract: Due to the rarity of metastatic or locally advanced basal cell carcinoma (mBCC, laBCC), most of the data regarding their characteristics come from single case reports and small series. None have performed a thorough evaluation of the histologic parameters of mBCC. To address this gap, we searched the entire medical record at Vanderbilt University Medical Center to identify potential cases of locally advanced or metastatic BCC occurring between 1984 and January 2019. A retrospective chart review was performed of all identified patients to determine case status.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.886
      Issue No: Vol. 140, No. 7 (2020)
       
  • 869 Establishment of a model of ras oncogene induced senescence in
           endothelial cells
    • Authors: A. Costa; M. Teichmann, M. Bonner, J. Mackelfresh, L. Gilbert, S. Rao, J. Arbiser
      Abstract: Oncogene induced senescence underlies many processes in dermatology, such as driver mutation induced vascular malformations, nevi, and seborrheic keratoses. These processes are difficult to study given that cells with driver oncogenes rapidly senescence in culture, whether the cells are transduced with a driver oncogene or cultured from a primary lesion. In order to provide a model easily accessible to the research community, we have established a model of oncogene induced senescence through the sequential introduction of a temperature sensitive large T oncogene followed by oncogenic H-ras into microvascular endothelial cells.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.885
      Issue No: Vol. 140, No. 7 (2020)
       
  • 868 Pathogenesis based therapy improves cutaneous abnormalities in
           porokeratosis- A pilot study
    • Authors: L. Azmony; Q. Sun, C. Hamilton, Y.H. Lim, J.S. Leventhal, A.S. Paller, K. Choate
      Abstract: Porokeratosis is a heterogenous group of keratinization disorders associated with mevalonate pathway gene mutations. Treatments options for the disease are few and often ineffective. On the basis of the possible pathogenic role of pathway end-product deficiency (cholesterol) and accumulation of toxic precursors, we studied the efficacy of topical lovastatin/cholesterol in different variants of porokeratosis. We recruited 1 patient with disseminated superficial actinic porokeratosis (DSAP), 2 patients with porokeratosis palmaris et plantaris disseminate (PPPD) and 2 patients with linear porokeratosis (LP).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.884
      Issue No: Vol. 140, No. 7 (2020)
       
  • 867 Direct mechanical measurements of skin to quantify evolution of
           sclerotic disease
    • Authors: L.X. Baker; F. Chen, Y. Ssempijja, L. Dellalana, A. Vain, M. Jagasia, E. Tkaczyk
      Abstract: There is an unmet need for objective cutaneous measurements to track the progression of sclerosing diseases, such as chronic graft-versus-host disease (cGVHD). The handheld Myoton device extracts biomechanical parameters of stiffness, frequency, and relaxation time characterizing soft tissue through its damped oscillation response to a mechanical micro-impulse. Cutaneous measurements using Myoton have differentiated sclerotic cGVHD patients from post-transplant controls. In a prospective longitudinal pilot study, we assessed Myoton’s ability to numerically monitor sclerosis over time.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.883
      Issue No: Vol. 140, No. 7 (2020)
       
  • 866 Baseline differences in circulation between early vs late responder
           patients with vitiligo treated with ruxolitinib cream
    • Authors: M. Howell; Y. Hu, Z. Zhu, K. Sun, F. Kuo, K. Butler, S. Owens
      Abstract: Vitiligo is a chronic, inflammatory skin disease characterized by increased interferon-gamma signaling through Janus kinase (JAK) 1 and JAK2 and subsequent activation of CD8+ T cells, which target melanocytes resulting in areas of depigmentation. Ruxolitinib cream, a JAK1/JAK2 inhibitor, is under investigation for vitiligo treatment in a 52-week, randomized, double-blind, phase 2 study (NCT03099304). Significantly more patients treated with ruxolitinib cream vs vehicle achieved ≥50% improvement in facial Vitiligo Area Scoring Index (F-VASI50) at Week 24 (primary endpoint); at Week 52, patients treated with ruxolitinib cream 1.5% twice daily (BID) attained the highest F-VASI50 response (57.6%).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.882
      Issue No: Vol. 140, No. 7 (2020)
       
  • 865 Immunotherapy in advanced Merkel cell carcinoma: Frequent responses
           but a potentially concerning recurrence rate after therapy discontinuation
           
    • Authors: Y. Moshiri; N. Alexander, T. Akaike, C. Doolittle-Amieva, T. Pulliam, L. Zaba, P. Nghiem
      Abstract: Merkel cell carcinoma (MCC) is an aggressive skin cancer that often recurs. MCC tumors can respond to PD-1 pathway inhibitors rapidly, however, it is unclear how often these responses persist after discontinuation of therapy. We retrospectively assessed 159 persons with advanced MCC treated with first-line anti-PD-(L)1 agents. Non-responders were defined as those with progressive disease (PD) or stable disease (SD), while responders had partial response (PR), or complete response (CR), based on clinician assessment.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.881
      Issue No: Vol. 140, No. 7 (2020)
       
  • 864 Lesional immune cells in cutaneous acute graft-versus-host disease: A
           prospective cohort study
    • Authors: R. Sennett; B. Jama, B. Hinds, D. Tzachanis, G. Morris, A. Marsch
      Abstract: Hematopoietic stem cell transplant (HSCT) is an intricate process that carries a risk of similarly convoluted complications, including graft-versus-host-disease (GVHD). Acute and chronic GVHD are distinct entities, defined by a combination of historical, clinical and pathologic data, but both are generally thought to stem from self-propagating aberrantly activated immune cells inflicting end organ damage, with the potential to cause significant morbidity and mortality. Survival rates after HSCT have improved significantly over the past few decades, but GVHD remains a major hurdle in improving the efficacy and safety of transplant.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.880
      Issue No: Vol. 140, No. 7 (2020)
       
  • 863 Role of STAT6 in advanced-stage cutaneous T-cell lymphoma
    • Authors: A. Gaydosik; D. Queen, O.E. Akilov, L. Geskin, P. Fuschiotti
      Abstract: Cutaneous T-cell lymphomas (CTCL) are a heterogeneous group of lymphoproliferative disorders derived from skin-homing memory T cells. Mycosis fungoides (MF) and Sezary syndrome (SS) are the most common subtypes. Aberrant cytokine expression in the MF/SS tumor microenvironment (TME) is a major factor in disease pathogenesis and progression. We have previously shown that MF/SS malignant lymphocytes produce high levels of IL-13, which acts as an autocrine factor for tumor cells and suppresses tumor-cell immunosurveillance.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.879
      Issue No: Vol. 140, No. 7 (2020)
       
  • 862 Thioredoxin regenerates elastic fibers in the dermis
    • Authors: T. Tohgasaki; S. Kondo, S. Nishizawa, X. Yu, S. Ishiwatari, T. Sakurai
      Abstract: Wrinkles and sagging occurring with aging affect the human physical appearance, resulting in poor quality of life. Therefore, care methods for these need to be improved. Wrinkles and sagging are thought to be caused by alterations in the internal skin structure; however, the specific morphological alterations and molecular mechanisms remain unclear. Previously, we observed the three-dimensional (3D) structure of the elastic fibers in the dermis by using tissue decolorization technology, and established a novel computational method to analyze its structural characteristics (volume, surface area, blanch numbers, length, diameter, and straightness).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.878
      Issue No: Vol. 140, No. 7 (2020)
       
  • 861 Intravenous gentamicin therapy for junctional epidermolysis bullosa
           patients harboring nonsense mutations
    • Authors: M. Hao; R. Antaya, J. Cogan, C. Hamilton, Y. Hou, A. Kwong, D. Woodley, M. Chen
      Abstract: Generalized severe junctional epidermolysis bullosa (GS-JEB) is an incurable and fatal inherited blistering skin disease most commonly caused by nonsense mutations in LAMA3, LAMB3, or LAMC2 genes. These mutations impair the ability to produce functional laminin 332, needed for epidermal-dermal adherence. Previously, we showed that topical gentamicin therapy generated new, functional laminin 332 and improved wound healing in GS-JEB patients. Although effective, topical administration of gentamicin to the entire skin surface is cumbersome and would not treat mucosal sites, including the upper respiratory tract.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.877
      Issue No: Vol. 140, No. 7 (2020)
       
  • 860 Microbiome therapy of atopic dermatitis by application of rationally
           selected human commensal skin bacteria
    • Authors: T. Nakatsuji; T. Hata, L. Tong, J. Cheng, F. Shafiq, A. Butcher, A. Spergel, K. Johnson, B. Jepson, A. Calatroni, P. Taylor, D. Leung, R.L. Gallo
      Abstract: Disease severity in atopic dermatitis (AD) is negatively influenced by S. aureus (SA). SA can be inhibited by antimicrobial molecules made by some strains of coagulase-negative Staphylococcus (AM+CoNS). A lack of AM+CoNS correlates with SA abundance and disease severity in AD. A previous clinical trial showed autologous AM+CoNS could reduce SA on AD skin within 24 hrs. Recently, some CoNS were also found to inhibit expression of SA toxins by blocking quorum sensing through production of autoinducing peptides (AIPs).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.876
      Issue No: Vol. 140, No. 7 (2020)
       
  • 859 Molecular characterization of mucosal lichen planus
    • Authors: A.N. Privalle; A. Meves, J. Lehman, R. Guo, J. Sartori-Valinotti
      Abstract: Lichen planus (LP) is an inflammatory mucocutaneous disease. The precise pathogenesis remains elusive. A critical event in the initiation of immune responses in LP lesions is for memory T cells to migrate from the circulation into the skin leading to cytokine/chemokine release (i.e. IFN-γ, IL-1β, IL-6, IL-8, IL-17, JAK3 and TNF-α). We sought to quantitatively examine the differential expression of various molecular mediators in idiopathic mucosal lichen planus and to test if the expression pattern is anatomic site-specific.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.875
      Issue No: Vol. 140, No. 7 (2020)
       
  • 858 A point-of-care, real time artificial intelligence system to support
           clinician diagnosis of a wide range of skin diseases
    • Authors: M. Zhang; B. Dulmage, K. Tegtmeyer, M. Colavincenzo, S. Xu
      Abstract: Diagnosing skin disease is challenging for non-dermatologists. Artificial intelligence (AI) clinical decision tools offer the potential to improve diagnostic accuracy. While previous AI tools have been used to classify skin cancers, there are limited technologies able to provide diagnostic support for both neoplastic and inflammatory skin conditions. Thus, we present the development and early validation of a point-of-care, real time AI tool that provides morphological classification of a wide range of skin diseases.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.874
      Issue No: Vol. 140, No. 7 (2020)
       
  • 857 An in vitro assay of inflammatory monocyte-keratinocyte activation
           predicts in vivo activity of BET inhibitors in a preclinical model of
           psoriasis
    • Authors: X. Wu; Z. Shi, D. Hsu, M. Huynh, D. Yamada, J. Chong, L. Mendoza, S. Hwang
      Abstract: Bromodomain and extra-terminal (BET) proteins perform a key role in epigenetic control of gene expression. Targeting BET bromodomains has been proposed in Th17-mediated autoimmune diseases such as psoriasis, which involves interplay between monocyte-macrophage cells and keratinocytes. To determine if we could create an effective in vitro model to screen BET inhibitors that would have activity in blocking psoriasiform dermatitis (PsD) in vivo, we co-cultured HaCaT keratinocytes with U937, a myeloid monocyte cell line which expresses TNF-α.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.873
      Issue No: Vol. 140, No. 7 (2020)
       
  • 856 Arthritis-associated pyoderma gangrenosum: A systematic review
    • Authors: E. Sawka; M. Friedman, A. Ortega-Loayza
      Abstract: Pyoderma gangrenosum (PG) is a rapidly-progressive neutrophilic dermatosis commonly associated with systemic inflammatory diseases. Our understanding of the relationship between PG and arthritis is lacking and the literature is currently dominated by case series and reports. We performed a systematic review in PubMed from 1950 to 2019 to determine the incidence of inflammatory arthritis among patients presenting with PG, the most common types of arthritis present in PG patients, and trends in treatment and outcomes.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.872
      Issue No: Vol. 140, No. 7 (2020)
       
  • 855 Dietary grape intake protects against UV damage in humans by
           augmenting DNA repair
    • Authors: A.S. Oak; R. Shafi, M. Elsayed, B. Mishra, L. Saag, A.T. Slominski, S. Bae, M. Athar, C. Elmets
      Abstract: Compounds found in grapes, such as ellagic acid, resveratrol and polyphenols, protect against photodamage in animal models. The purpose of this single-group, open-label clinical study was to determine whether grapes had a similar effect in humans. The minimal erythema dose (MED) was determined in 19 healthy volunteers, (Fitzpatrick skin types I-III), and biopsies were taken from sun-exposed and non-sun-exposed skin. They were instructed to consume 25 g of freeze-dried grape powder 3x/day for 2 weeks.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.871
      Issue No: Vol. 140, No. 7 (2020)
       
  • 854 Functional drug screening identifies candidate synergistic
           combinations for CTCL therapy
    • Authors: S. Yumeen; F.N. Mirza, J.M. Lewis, A.O. King, S. Kim, K.R. Carlson, S. Umlauf, Y. Surovtseva, F.M. Foss, M. Girardi
      Abstract: Cutaneous T cell lymphoma (CTCL) is a malignancy of T lymphocytes that may involve the peripheral blood in advanced stages. Reported gene copy number alterations and single nucleotide variants in CTCL have suggested therapeutic targets in altered and aberrantly activated pathways. Combination targeted agent treatment strategies are emerging as an approach to increase efficacy and reduce risk via drug synergy. We aimed to investigate the potential roles of single and combination approaches utilizing agents targeting JAK, BCL2, BET, and HDAC in CTCL cells.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.870
      Issue No: Vol. 140, No. 7 (2020)
       
  • 852 Natural killer cell deficiency reveals a novel immunotherapy strategy
           for atopic dermatitis
    • Authors: M.R. Mack; J.R. Brestoff, M.M. Berrien-Elliott, T.B. Yang, A.M. Trier, P.L. Collins, H. Niu, N.D. Bodet, J.A. Wagner, E. Park, A.Z. Xu, F. Wang, M. McCullen, R. Chibnall, M.L. Council, D.J. Margolis, D. Sheinbein, E. Vivier, P. Lovato, M. Cella, M. Colonna, W.M. Yokoyama, E.M. Oltz, T.A. Fehniger, B. Kim
      Abstract: Atopic dermatitis (AD) is a widespread, chronic skin disease associated with aberrant allergic inflammation. While current treatments involve either broad or targeted immunosuppression strategies, enhancing aspects of the immune system to control disease remains largely untested. We demonstrate that patients with AD harbor a blood natural killer (NK) cell deficiency that has both diagnostic value and improves with therapy. Multidimensional CyTOF analysis and RNA-seq profiling of patients' peripheral blood NK cells revealed subset-level changes associated with activation-induced cell death.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.868
      Issue No: Vol. 140, No. 7 (2020)
       
  • 851 Acetyl zingerone opposes deleterious effects of skin aging by
           bolstering matrisome synthesis, neutralizing oxidative stress and
           inhibiting DNA damage
    • Authors: W.R. Swindell; T. Meyer, S. Premi, K. Bojanowski, R. Chaudhuri
      Abstract: Acetyl zingerone (AZ) is a new molecule structurally related to Zingerone (Z), a naturally occurring phenolic alkanone found in ginger, but designed for improved stability and antioxidant function. In microarray studies performed using reconstituted human epidermis, both Z and AZ increased Notch pathway gene expression (NOTCH1, MAML3) and decreased expression of genes linked to ECM disassembly (MMP3, CTSV, NOXO1) and reactive oxygen species metabolism (PMAIP1, ARG2). Although Z and AZ each inhibited in vitro MMP-1, MMP-3 and MMP-12 activity, inhibition of MMP-3 and MMP-12 was greater with AZ.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.867
      Issue No: Vol. 140, No. 7 (2020)
       
  • 849 Development of an ex vivo rabbit eye model for bacterial
           conjunctivitis
    • Authors: A. Caserta; J.E. Neil, J. Lenn, M. Brown
      Abstract: This ex vivo rabbit ocular model was designed to mimic the common eye disease bacterial conjunctivitis and evaluate the response of ocular therapeutics. Fresh rabbit eyes were extracted, either whole globe or dissected into cornea, surrounding sclera and lens, and mounted into agar/media substrate. The eyes were then cultured at 37°C for up to 3 days with LPS/PGN stimulation to determine the optimal method to mimic bacterial conjunctivitis inflammation and to evaluate the associated biomarkers by RT-qPCR.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.865
      Issue No: Vol. 140, No. 7 (2020)
       
  • 848 Clinical and immunological profiles of BP-specific IgE autoantibodies
           in bullous pemphigoid
    • Authors: Y. Shih; H. Yuan, J. Zheng, M. Pan
      Abstract: A subset of bullous pemphigoid (BP) patients show anti-BP180 and anti-BP230 IgE in the serum, yet the data about the diagnostic value of anti-BP230 IgE and the clinical presentation of BP remains inconclusive.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.864
      Issue No: Vol. 140, No. 7 (2020)
       
  • 847 Redness has higher interrater reproducibility than body surface area
           in measuring extent of photographed cutaneous graft-versus-host disease
    • Authors: K. Parks; Z. Khera, X. Liu, T. Reasat, L.X. Baker, M. Jagasia, B. Dawant, E. Tkaczyk
      Abstract: Reliability for estimating disease body surface area (BSA) has interested investigators but is not known for photos. We evaluated accuracy and agreement of marking active disease from 3D photos of cutaneous chronic graft-versus-host disease (cGVHD). Prior to the study, three non-physician trainees (medical student KP, post-doc XL and PhD student TR) read background cGVHD literature. The Canfield Vectra H1 camera was used to capture a set of 15 3D photos from 8 patients, including 7 body sites. Then before and after 6 months of instruction by a board-certified dermatologist (ERT), each trainee used Vectra analysis software to demarcate the active areas of cGVHD in the set.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.863
      Issue No: Vol. 140, No. 7 (2020)
       
  • 845 Management of pyoderma gangrenosum in the perioperative period
    • Authors: A. Ortega-Loayza; C. Haag, L. Bacik, E. Latour, D. Morse, N. Fett
      Abstract: Pyoderma gangrenosum (PG) classically presents with the rapid evolution of painful skin ulcerations in an acute inflammatory stage, which is typically treated with immunosuppression. A subset of PG patients treated with immunosuppression can develop refractory, non-inflammatory ulcers, of which there are reports describing successful surgical outcomes (e.g. healing). However, the potential for pathergy can complicate disease management, as surgical procedures are generally contraindicated in patients with PG.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.861
      Issue No: Vol. 140, No. 7 (2020)
       
  • 844 Increased urine phthalate metabolite levels associated with eczema
           diagnosis
    • Authors: S.P. Patel; R. Khanna, K. Williams, J. Choi, Y. Semenov, S. Kwatra
      Abstract: The skin is exposed to unprecedented levels of pollution which can activate inflammatory pathways, affect the immune system, and induce oxidative stress. In particular, the use of phthalates as plasticizer in consumer products has created health concerns since the pollutant is believed to cause hormonal and immune dysregulation. We thus investigated the association between phthalate exposure and eczema. Using the National Health and Nutrition Examination Survey, we conducted a cross-sectional analysis to investigate the association between urinary phthalate metabolite levels and eczema.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.860
      Issue No: Vol. 140, No. 7 (2020)
       
  • 843 Seeing water in the skin: Hyperspectral imaging in the short-wave
           infrared
    • Authors: L. Shmuylovich; D.K. Mishra, H. Hurbon, A. Yu, T. Du, T. Wang, M. Berezin
      Abstract: Noninvasive skin imaging typically relies on light absorption and scatter of chromophores like melanin, hemoglobin, and collagen in the visible and near-infrared. Low absorption makes water essentially invisible in this spectral range. Because fluid accumulation is a key pathophysiologic component of many skin diseases, an imaging modality where water has high absorption would be useful. The short-wave infrared (SWIR) range (900-1700 nm) has high lipid and water absorption and relatively low melanin and hemoglobin absorption.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.859
      Issue No: Vol. 140, No. 7 (2020)
       
  • 842 Increased leukocyte pausing in the upper dermal microvasculature of
           cutaneous acute graft-versus-host disease patients by noninvasive
           reflectance confocal video microscopy
    • Authors: J.R. Patrinely; I. Saknite, Z. Zhao, M. Byrne, M. Jagasia, E. Tkaczyk
      Abstract: Acute graft versus host disease (aGVHD), one of the most common and potentially deadly complications of hematopoietic cell transplantation (HCT), is often difficult to diagnose with traditional clinical and histopathologic examination. Through five quantitative parameters extracted from noninvasive reflectance confocal microscopy videos, we compared the differences in upper dermal microvasculature of patients post-HCT with skin aGVHD (N=10) and with no organ aGVHD (post-HCT controls, N=10). We used a clinical reflectance confocal microscope, the Vivascope 1500 (Caliber I.D., Rochester, NY), to image volar forearm and upper chest blood vessels.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.858
      Issue No: Vol. 140, No. 7 (2020)
       
  • 841 Machine learning (ML) predictive algorithm for childhood-onset chronic
           pruritic dermatoses (CPD) identifies acrylamide and glycidamide as itch
           modulators
    • Authors: S. Wongvibulsin; Y. Semenov, R. Khanna, S. Zeger, S. Kwatra
      Abstract: The factors responsible for childhood-onset CPD are not fully known. We hypothesize that environmental factors play a role and ML algorithms can increase understanding of childhood-onset CPD. We employ the classification random forest (RF) algorithm to systematically evaluate the association between CPD and environmental exposures in children (ages 16 and younger) in the 2005-2006 NHANES. We define incident CPD as presence of an itchy rash for at least 6 months with first rash occurrence within the past year.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.857
      Issue No: Vol. 140, No. 7 (2020)
       
  • 839 Spirulina stimulates inflammatory cytokine production in
           dermatomyositis in vitro
    • Authors: C. Bax; Y. Li, A. Ravishankar, S. Maddukuri, J. Patel, D. Yan, V. Werth
      Abstract: Spirulina, a popular herbal supplement, stimulates the immune system, as determined by in vitro and in vivo studies. Our recent epidemiologic data suggest that Spirulina is associated with the onset or exacerbation of pre-existing autoimmune skin diseases, such as Dermatomyositis (DM). The purpose of this study was to 1) investigate the immunostimulatory effects of Spirulina and 2) characterize the role of the Stimulator of interferon genes (STING) and TLR4 pathways. PBMCs were isolated from DM and normal controls and stimulated with increasing concentrations of Spirulina supernatant (0, 0.3, and 1 mg/ml) or with Spirulina supernatant and STING antagonist, H-151, or TLR4 antagonist for 18 hours.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.855
      Issue No: Vol. 140, No. 7 (2020)
       
  • 838 A guideline improves interrater reliability of assessing leukocyte
           motion in vivo in human skin
    • Authors: Z. Zhao; J.R. Patrinely, I. Saknite, M. Byrne, M. Jagasia, E. Tkaczyk
      Abstract: Reflectance confocal microscopy (RCM) enables in vivo, real-time visualization of adherent and rolling leukocytes in human skin microvasculature. We found that adherent and rolling leukocytes are increased in cutaneous graft-versus-host disease (GVHD) [1]; however, the interrater reliability of these manual counts is unknown. We acquired 1164 RCM videos from 45 subjects in a cross-sectional study: 10 healthy, 15 GVHD patients, and 20 post-hematopoietic cell transplant controls. Based on standard definitions (adherent: paused>= 30 seconds; rolling: rotation on its axis along the vessel wall), two trained raters blinded to diagnosis independently counted adherent and rolling leukocytes in an initial set of 88 videos.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.854
      Issue No: Vol. 140, No. 7 (2020)
       
  • 837 Micro-regional transcriptome reveals local dermal-epidermal
           intercorrelation in atopic dermatitis
    • Authors: T. Miyai; H. Kawasaki, M. Hosokawa, H. Matsunaga, R. Satoh, A. Sekita, H. Takeyama, M. Amagai, H. Koseki
      Abstract: Skin consists of a coordinated structure and multiple appendages, all of which could be affected under specific disease conditions such as atopic dermatitis (AD). Therefore, spatial information of gene expression should be essential for understanding the pathogenesis of skin inflammation. To obtain spatial profiles of the skin transcriptome, we have performed site-specific RNA-seq of 100 μm-diameter regions obtained by punch microdissection in wild-type (WT) and AD model mice. We collected 120 spots (epidermis, dermis, and hair follicle) in total from 14 proximal areas (WT: n=4, non-lesion (NL): n=6, and lesion (LS): n=4) on the frozen tissue sections.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.853
      Issue No: Vol. 140, No. 7 (2020)
       
  • 836 Calibration performance of deep neural networks for image
           classification declines on real-world, versus curated, test sets
    • Authors: A.T. Young; J. Pfau, M.J. Keiser, M. Wei
      Abstract: Deep convolutional neural networks (CNNs) perform on par with or better than dermatologists for melanoma image classification. Rather than yes-no predictions, CNNs output a probability distribution over a set of predefined disease classes, whereby prediction confidence is the max probability over the disease classes. A well-calibrated CNN, such that confidence forecasts observed prediction accuracy, is critical for clinical application. However, CNNs tend to be overconfident and therefore must first be calibrated, a topic not yet studied for image-based skin lesion diagnosis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.852
      Issue No: Vol. 140, No. 7 (2020)
       
  • 833 No detectable systemic absorption of topically applied 0.02% and 0.04%
           chlormethine (CL) gel in patients with mycosis-fungoides cutaneous T-cell
           lymphoma (MF-CTCL)
    • Authors: C. Querfeld; J. Scarisbrick, P. Quaglino, E. Papadavid, P. Ortiz Romero
      Abstract: CL (also known as mechlorethamine) 0.016% w/w gel (equivalent to 0.02% CL HCl) is an approved skin-directed therapy for treatment of MF-CTCL. The 201 study evaluated efficacy and safety of CL 0.02% gel vs equal strength CL ointment. The 202 study evaluated response and tolerability in patients (pts) from the 201 study who did not achieve a CR after 0.02% topical treatment and used 0.04% gel formulation. Bioanalysis of blood samples from pts treated with 0.02% or 0.04% CL gel was used to assess if systemic absorption occurred.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.849
      Issue No: Vol. 140, No. 7 (2020)
       
  • 832 Variability in secretion of growth factors by platelets is intrinsic
           to alopecia patients undergoing platelet-rich plasma therapy
    • Authors: J.A. Shaik; N. Estharabadi, R. Farah, M.K. Hordinsky
      Abstract: Platelet α-granules release growth factors (GFs) that promote healing and tissue regeneration by stimulating cell proliferation and differentiation. Platelet-rich plasma (PRP) prepared from autologous blood has shown to be beneficial in treating alopecia, however, clinical response can be inconsistent. Due to several fold enrichment of platelets secreting large quantities of GFs following PRP injections, variable GF secretion by platelets between patients may contribute to inconsistent clinical response.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.847
      Issue No: Vol. 140, No. 7 (2020)
       
  • 831 ARHGAP29 regulates keratinocyte migration in vitro but not in vivo
    • Authors: T. Reeb; A. Stein, L. Rhea, D. Wessels, D. Soll, M. Dunnwald
      Abstract: ARHGAP29 is a Rho GTPase Activating Protein with a high affinity for the small GTPase RhoA. In endothelial cells, ARHGAP29 regulates tubulogenesis and endothelial barrier function. However, very little is know about the role of ARHGAP29 in keratinocytes. We previously demonstrated that ARHGAP29 was reduced in Irf6-deficient skin and keratinocytes. Beause IRF6 is required for keratinocyte migration, we hypothesize that ARHGAP29 also regulates keratinocyte migration. To test this hypothesis, we used CRISPR-Cas9 technology to generate keratinocyte cell lines possessing two mutant alleles for ARHGAP29.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.846
      Issue No: Vol. 140, No. 7 (2020)
       
  • 829 Single-cell approaches to uncover adipocyte precursor heterogeneity
           and differentiation mechanisms in the skin
    • Authors: G. Rivera; K. Kamimoto, E. Butka, W. Kong, S. Morris
      Abstract: Dermal white adipose tissue (dWAT) is key for multiple processes in the skin, ranging from immune response to hair growth. The generation of mature adipocytes in the skin relies on the proliferation and differentiation of adipocyte precursors (APCs). APCs can be identified by the expression of surface markers such as CD29, CD34, Sca1, and CD24, however the true extent of APC heterogeneity remains largely unknown. Using single-cell RNA sequencing (scRNA-seq) we reveal an unprecedented degree of heterogeneity in skin APCs.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.844
      Issue No: Vol. 140, No. 7 (2020)
       
  • 828 Src-family kinases enhance corneal wound healing and compensate for
           neurotrophic keratopathy
    • Authors: V. Lee; R.T. Kweon, L.T. Wushanely, W.T. Foos, C.T. Lin, J.T. Seykora
      Abstract: Corneal wounds are a leading cause of blindness and therapeutic approaches that target early epithelial wound healing can minimize the risk of blindness. Preliminary studies of corneal wounds showed increased SFK activity at the wound edge. Therefore, we hypothesized that increased SFK activity may promote corneal epithelial wound healing in vitro and in vivo. Our data show that in vitro wound healing in primary human corneal epithelial cells (HCEC) is enhanced when SFK levels are increased by sodium orthovanadate (NaVO4).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.843
      Issue No: Vol. 140, No. 7 (2020)
       
  • 826 Ultra-low profile, soft pressure sensors with wireless communication
           for wound healing applications
    • Authors: Y. Park; K. Kwon, J. Kwak, S. Kwak, D. Yang, D. Pontes, M. Zhang, S. Xu, J. Rogers
      Abstract: Compression garments (CGs) such as bandages and stockings are critical in the management of a wide range of lower extremity conditions, particularly venous leg ulcers (VLUs). In order for CGs to deliver therapeutic benefit, the interface pressure between the fabric and the skin much typically reach at least 30 mmHg. However, this threshold value is highly sensitive to the type of CG material used, correct placement by a trained healthcare provider, body position, and leg volume changes. Currently, PicoPress® (Microlabitalia, Padua, Italy) is the gold-standard tool to measure interface pressure.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.841
      Issue No: Vol. 140, No. 7 (2020)
       
  • 825 Aquaphilus dolomiae S0, a thermal spring water active compound,
           showing broad repairing properties of on in vitro models of injured skin
    • Authors: M. Noizet; P. Bianchi, M. Galliano, A. Caruana, J. Brandner, S. Bessou-Touya, H. Duplan
      Abstract: The clinical efficacy of Avène thermal spring water in atopic dermatitis, psoriasis or in reducing erythema post laser resurfacing have been shown by several studies. In addition to these soothing and immunomodulatory properties, in-vitro experiments have also demonstrated effects of Avène thermal spring water on stimulation of keratinocyte differentiation and improvement of membrane fluidity, suggesting a potential effect on skin barrier and repair. An investigation of the deep aquifer of the Avène thermal spring water pointed out a new microorganism as a potential source of these unique properties.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.840
      Issue No: Vol. 140, No. 7 (2020)
       
  • 820 Filling Injection of Platelet-rich Plasma Gel as A New Method to Treat
           En Coup de Sabre Scleroderma
    • Authors: T. Huang; G. Hu, B. Yang
      Abstract: Platelet-rich Plasma (PRP) plays an important role on the tissue regeneration process. A new preparatioin method of PRP provides an filling injection product which was named Platelet-rich Plasma Gel (PRPG) and could provied synergic effects on skin elasticity, dermal remodeling and re-vascularization. The aim of this pilot study was to assess the clinical benefit of PRPG filling injection on the treatment of en coup de sabre scleroderma. Five patients who were diagnosed under the confirmation of histopathology, and assessed by ultrasonography.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.835
      Issue No: Vol. 140, No. 7 (2020)
       
  • 819 Involvement of papillary and reticular fibroblasts in dermal
           angiogenesis
    • Authors: E. Aymard; A. Mauroux, C. Monnot, S. Germain, F. Ruggiero, L. Muller, B. Closs
      Abstract: Wound healing is a multi-step complex mechanism during which dermal angiogenesis is a critical factor. Human dermis has a complex architecture, divided into papillary and reticular dermis which are characterized by distinct extracellular matrix (ECM) and vasculature. Nowadays, the contribution of each subtype of fibroblasts in the generation of specific ECM and vasculature is poorly documented. The aim was to determine if papillary and reticular fibroblasts generate specific 3D microenvironments and then evaluate their impact on angiogenesis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.834
      Issue No: Vol. 140, No. 7 (2020)
       
  • 816 Dual LSD1/HDAC inhibition accelerates skin wound healing
    • Authors: M. Kida; M. Wu, P. Cole, V. Falanga, A. Sharov, R. Alani
      Abstract: The epigenetic machinery regulates epidermal homeostasis and keratinocyte differentiation is normal skin, while epigenetic control of wound healing remains relatively underexplored. The HDAC1/2 and LSD1 enzymatic activities within the CoREST complex are commonly associated with silencing of gene expression. Corin is a synthetic hybrid agent derived from the HDAC inhibitor (MS-275) and LSD1 inhibitor. In this study, using in vivo mouse tail wound healing model, we found that HDAC1 and LSD1 expression levels in epidermal keratinocytes show dynamic changes after injury compared to uninjured skin.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.831
      Issue No: Vol. 140, No. 7 (2020)
       
  • 815 Targeting GM3 synthesis improves wound healing in human diabetic skin
           equivalents
    • Authors: T.R. Holmes; K. Lewandowski, K.R. Kwan, M.S. Bonkowski, A.S. Paller
      Abstract: Chronic foot ulcers are a major cause of morbidity and mortality in 25% of individuals with type 2 diabetes (T2D). Our laboratory has previously shown increased expression of sphingolipid GM3 and GM3 synthase (GM3S) in human diabetic foot skin. Preventing increases in GM3 in a diet-induced mouse diabetic model or in high glucose-treated 2D keratinocyte cultures improved wound healing and scratch wound closure, respectively. To further test the role of increases in GM3/GM3S on wound healing in human T2D, a diabetic 3D human skin equivalent (HSE) model using diabetic foot ulcer fibroblasts (DFUFs) and normal keratinocytes was generated.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.830
      Issue No: Vol. 140, No. 7 (2020)
       
  • 814 Circadian factors BMAL1 and CLOCK control transcriptional innate
           antiviral immunity programs in response to skin wounding
    • Authors: S. Kirchner; V. Lei, M. Coates, C. Handfield, D. Corcoran, X. Ling, J. Shannon, P. Rosa Coutinho Goulart Borges Mariottoni, D. Hughes, D. Waters, K. Dzirasa, A.S. MacLeod
      Abstract: Antiviral proteins (AVPs) including the oligoadenylate-synthase (OAS) and Interferon induced transmembrane protein (IFITM) families have protective roles within the innate immune system. However, little is known about their regulation in skin. BMAL1 and CLOCK, regulators of the circadian rhythm, have known importance in a number of immune functions. We hypothesized that the circadian clock may regulate cutaneous AVP expression. We demonstrate that murine skin displays homeostatic oscillations of AVP expression through the day, and that AVPs exhibit modest rhythmic expression in primary human keratinocytes post-circadian synchronization using serum starvation or dexamethasone shock with a periodicity of 20 to 24 hours.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.829
      Issue No: Vol. 140, No. 7 (2020)
       
  • 813 Metabolic products of Cutibacterium acnes protect against cutaneous
           biofilm formation by Staphylococci
    • Authors: K. Nakamura; A. ONeill, M. Williams, L. Cau, T. Nakatsuji, R.L. Gallo
      Abstract: Bacterial biofilms are a major factor in delayed wound healing. Many staphylococcal species can form biofilms that have the potential to harm the host. DNA sequencing and qPCR of 16S RNA from bacteria isolated from human hair follicles by laser capture microdissection has shown that Staphylococci are highly abundant in the normal human follicles and co-exist with C. acnes. However, despite the high density of Staphylococci in follicles, biofilms seldom occur on healthy skin. We hypothesized that interactions between species in the commensal skin microbiome may regulate biofilm formation.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.828
      Issue No: Vol. 140, No. 7 (2020)
       
  • 812 Multi-view light sheet florescence microscopy (LSFM) for imaging
           cellular self-assembly in spheroids of human hair follicle dermal papilla
           cells and keratinocytes
    • Authors: S. Li; F.W. Lischka, P. Klover, J. Wang, I.M. Herman, T. Darling
      Abstract: Heterotypic spheroids are used to study mesenchymal-epithelial interactions important for skin development and regeneration. In order to investigate the behavior and interaction of human hair follicle dermal papilla cells (DPC) and keratinocytes during in vitro three-dimensional (3D) co-culture, we labeled DPC and keratinocytes with two different fluorescent cell tracking dyes and prepared spheroids in DPC medium by hanging-drop technique. After incubation for 1 or 2 days, spheroids were live embedded in an agarose cylinder and image stacks were generated in multi-view mode from several angles using dual-sided LSFM.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.827
      Issue No: Vol. 140, No. 7 (2020)
       
  • 811 Nipple fibroblasts express low levels of fibrotic inducer DPP4 and
           facilitate wound healing with minimal scarring
    • Authors: S. Vargas; A. Filipowicz, M. Keel, H. Zhou, D. Spandau, M.J. Turner, J.G. Foley
      Abstract: Sites of specialized skin in humans, including the nipple-areola complex exhibit minimal scaring after wounding. The distinct epidermal and dermal features of these skin regions are induced and maintained by signaling from local fibroblasts. In contrast, trunk fibroblasts induce scarring after wounding and this is mediated by a subpopulation of that express high levels of dipeptidyl peptidase IV (DPP4/CD26). Profiling of transcripts of adult fibroblasts from a murine model of ectopic nipple development based on keratin 14 promoter driven parathyroid-related protein (KrP) transgene, identified increased levels of ovarian hormone receptors and matrix production, but decreased DPP4 and TGFB1 levels.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.826
      Issue No: Vol. 140, No. 7 (2020)
       
  • 809 Acne-associated strains of Cutibacterium acnes induces significantly
           higher MMP-1 expression, a potent mediator of extracellular matrix
           degradation
    • Authors: A. Awad; M. Qin, T. To, J. Kim
      Abstract: Scarring is a common sequelae of inflammatory acne vulgaris caused by the gram-positive bacterium, Cutibacterium acnes (C. acnes). Aberrant extracellular matrix remodeling seen in scarring may be due to upregulated activity of matrix metalloproteinases (MMPs) in the inflammatory response, resulting in loss of collagen deposition, increased tissue destruction, and imperfect repair during the wound healing process. C. acnes was shown previously to induce MMP-1 (collagenase) and MMP-9 (gelatinase) expression.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.824
      Issue No: Vol. 140, No. 7 (2020)
       
  • 808 Recombinant T. pallidum protein TP0136 promotes the migration of
           fibroblasts by inducing MCP-1/CCR2 expression through signalling involving
           the TLR4, ERK, JNK, PI3K and NF-κB signalling pathways
    • Authors: W. Ke
      Abstract: Background: Wound healing involve the migration of fibroblasts. The microenvironment of fibroblast migration is not beneficial for the survival of most pathogens due to lack of oxygen. However, for T. pallidum, the hypoxic environment is relatively suitable for survival. Ulceration lesion chancre, the early stage of syphilis, can heal spontaneously within a few weeks without treatment. The specific mechanism underlying this process is still unknown. Objective: Tp0136 is one of a few proteins produced by T.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.823
      Issue No: Vol. 140, No. 7 (2020)
       
  • 807 Topical type VII collagen increased elastic fiber formation,
           accelerated wound closure and reduced scarring of pigskin wounds
    • Authors: D. Woodley; J. Cogan, Y. Hou, M. Hao, A. Kwong, W. Li, M. Chen
      Abstract: Non-healing skin wounds are common and represent major medical, economic and social problems worldwide. Currently, there is a paucity of effective therapy for skin wounds. We have shown previously in several murine skin wound models that recombinant human type VII collagen (rhC7) dramatically accelerates wound closure and inhibits scarring. Murine skin wounds, however, heal much differently than human skin wounds. Therefore, in this study, we evaluated the effect of topical rhC7 on pigskin wounds.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.822
      Issue No: Vol. 140, No. 7 (2020)
       
  • 806 Bioprinted skin integrates and forms epidermal rete ridges in
           full-thickness wounds
    • Authors: A.M. Jorgensen; A. Gorkun, M. Varkey, C. Clouse, S. Lee, J.J. Yoo, S. Soker, A. Atala
      Abstract: Current tissue engineered skin fails to meet the need for skin replacement in full-thickness wounds. Bioprinting technology allows for fabrication of full-thickness skin with multiple cell types organized into biomimetic layers in vitro. The purpose of this study is to determine if bioprinted skin will integrate, form an epidermal barrier, and vascularize when implanted into full-thickness wounds on mice. Cells were isolated from human skin, expanded in vitro, suspended in a fibrinogen bioink, bioprinted to form a biomimetic tri-layer skin construct, and implanted onto 2.5 x 2.5cm full-thickness excisional wounds on mice.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.821
      Issue No: Vol. 140, No. 7 (2020)
       
  • 805 TEAD1 and TEAD3 play redundant roles in the regulation of human
           epidermal proliferation
    • Authors: J. Li; G.L. Sen
      Abstract: Hippo signaling pathway is important for regulating cell growth, proliferation and organ development. It functions through the Yes-associated protein 1 (YAP1), which acts with the TEAD family transcription factors to regulate essential genes for proliferation and cell growth. However, it is not known whether TEAD (1-4) genes play redundant or non-redundant role in the human epidermal tissue development. We knocked down each individual TEAD and in combinations in human keratinocytes and found that only double knockdown of TEAD1 and TEAD3 resulted in a significant decrease of CTGF and AXL mRNAs as well as other critical self-renewal and proliferation genes (BIRC5, FGFBP1, CCNA2, MYC, CDK1, CYR61).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.820
      Issue No: Vol. 140, No. 7 (2020)
       
  • 804 Overcoming wound healing complications of radiotherapy in breast
           dermal fibroblasts through the influence of pre-adipocytes
    • Authors: L.V. Trevor; K. Riches-Suman, A. Mahajan, J.M. Thornton
      Abstract: Impaired wound healing in irradiated tissue is a significant clinical problem which may be improved by stromal vascular fraction (SVF) containing pre-adipocytes. We studied the characteristics of dermal fibroblasts (DF)s derived from human breast skin exposed to radiotherapy to investigate challenges presented by irradiation prior to reconstruction, and whether the pre-adipocyte secretome has potential therapeutic benefit. Tissue collected from breast reduction (Ctrl), or reconstruction following 40 Gy hypofractionated radiotherapy (IR) was processed for histology, and isolation of primary DF and SVF cultures.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.819
      Issue No: Vol. 140, No. 7 (2020)
       
  • 803 A20 and its repressor DREAM expression govern susceptibility to
           fibrosis in systemic sclerosis
    • Authors: W. Wang; B. Claudia Schock, H. Abdala-Valencia, B. Jeong, S. Bale, R.B. Christmann, R. Marangoni, S. Berdnikovs, E. Herzog, J. Varga, S. Bhattacharyya
      Abstract: Recent GWAS have uncovered consistent genetic linkage of SSc fibrotic phenotypes with TNFAIP3, encoding the ubiquitin-editing enzyme A20. A20 has been previously implicated in negative regulation of innate immunity, and hypomorphic A20 variants are associated with autoimmunity in SLE, RA,and others.The transcription factor DREAM binds to the A20 promoter to repress expression. Unbiased transcriptome analysis of skin and lung biopsies from SSc patients showed significantly decreased A20 levels and robust anti-correlation with fibrotic TGF-beta signaling.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.818
      Issue No: Vol. 140, No. 7 (2020)
       
  • 802 Effects of cholera toxin and isoproterenol as cAMP stimulators for a
           psoriatic reconstructed skin model
    • Authors: S. Morin; M. Simard, G. Rioux, R. Pouliot
      Abstract: Psoriasis is a pathology mainly characterized by the hyperproliferation of epidermal cells, which leads to abnormal cell differentiation. A change in cyclic adenosine monophosphate (cAMP) levels could be causing this altered proliferation, as cAMP plays a major role in epidermis cellular growth. However, whether levels of cAMP in psoriatic skin are enhanced or decreased is a matter of much controversy. The aim of this study was therefore to quantify the levels of cAMP in psoriatic skin substitutes produced with two different cAMP enhancers, cholera toxin and isoproterenol, and to evaluate what impacts these levels have on the main characteristics of the pathology.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.817
      Issue No: Vol. 140, No. 7 (2020)
       
  • 800 The role of YAP/TAZ in the pathogenesis of skin fibrosis
    • Authors: L. Lu; N. Gao, X. Ma, Z. Liu, X. Wang, S. Yang, G. Han
      Abstract: YAP/TAZ are key molecules in the Hippo signaling pathway that regulate organ size and promote the development and progression of proliferative diseases such as tumors through the induction of cell growth and inhibition of apoptosis. Skin fibrosis is characterized by persistent proliferation of fibroblasts and excessive production of extracellular matrix. Given the key role of the Hippo signaling pathway in other cells, we speculate that YAP/TAZ may also be involved in the pathogenesis of fibrosis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.815
      Issue No: Vol. 140, No. 7 (2020)
       
  • 799 Acute IL-17A production by a 3D immunocompetent psoriatic skin
           substitute to study the interaction between epithelial and immune cells
    • Authors: G. Rioux; S. Morin, M. Simard, S. Guérin, R. Pouliot
      Abstract: Psoriasis is a chronic inflammatory dermatosis mediated by T cells through the IL-23/IL-17 axis. The hallmark cytokine of the Th17 cells, IL-17A, activates keratinocytes leading to the secretion of various chemokines that also account for leukocytes infiltration, thus producing an activation loop leading to the chronicization of the psoriatic lesions. The lack of suitable preclinical models reflecting the complex phenotype of this skin disease is a major obstacle to the further study of psoriasis pathogenesis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.814
      Issue No: Vol. 140, No. 7 (2020)
       
  • 796 Effect of novel disaccharide for construction of living skin
           equivalents
    • Authors: J. Muto; S. Fukuda, Y. Shirakata, T. Tsuda, E. Tan, X. Dai, K. Shiraishi, H. Mori, M. Murakami, S. Higashiyama, K. Sayama
      Abstract: Living skin equivalents (LSEs) containing well-differentiated keratinocytes cultivated on fibroblast-populated dermal substitutes have been used to treat burn wounds, skin defects of epidermolysis bullosa. The dermal matrix and the fibroblasts in LSEs modulate epidermal growth and differentiation through dynamic interactions. Various biomaterials have been used as dermal matrix substitutes, but the search has continued for an ideal matrix that is readily available and has minimal toxicity. The non-reducing disaccharide trehalose is a singular molecule, which has been conserved throughout evolution in prokaryotes, plants, invertebrates, but is absent in vertebrates including mammals.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.811
      Issue No: Vol. 140, No. 7 (2020)
       
  • 795 Bacteria induce skin regeneration
    • Authors: G. Wang; H. Liu, E.M. Wier, R. Ortines, N. Islam, A. Li, Y. Xue, Y. Wang, N. Archer, L.S. Miller, L. Garza
      Abstract: Wound Induced Hair follicle Neogenesis (WIHN) is a rare adult organogenesis model where stem cells form de novo hair follicles following full-thickness wounding. As wounds inevitably contact the skin microbiota, it is important to understand the role of the skin microbiome in WIHN. To do so, we modified bacterial burdens and tested WIHN. We used 3 levels of microbial burden to measure WIHN: For minimal bacteria loads, we used germ-free (GF) mice, applied antibiotic ointment (Neosporin) or frequent cage changes of standard specific pathogen free (SPF) mice housing.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.810
      Issue No: Vol. 140, No. 7 (2020)
       
  • 794 RNase L is a regeneration repressor gene
    • Authors: N. Islam; E.M. Wier, M. Alphonse, H. Liu, D. Kim, A. Li, S. Reddy, L.S. Miller, H. Weiliang, S. Lee, S. Kim, G. Wang, M. Kane, R. Silverman, L. Garza
      Abstract: When compared to animals across other phyla, mammals have restricted regeneration and more fibrosis. This limited regenerative capacity may reflect a loss of pro-regeneration programs or active suppression by genes functioning akin to tumor suppressors. To uncover the programs governing regeneration in mammals, we investigated Wound Induced Hair Neogenesis (WIHN), a rare example of regeneration in adult mammals. Through comprehensive screening of transcripts associated with WIHN—as well as after rejuvenation lasers in human subjects--, we found that the endoribonuclease RNase L associates with regeneration/rejuvenation, but actually functions as a powerful suppressor of regeneration.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.809
      Issue No: Vol. 140, No. 7 (2020)
       
  • 793 Biphasic modulated pulsed microcurrent treatment of skin
    • Authors: D. Kern; M. Riggs, H.E. Knaggs
      Abstract: Application of low level AC or DC current to the skin to assist drug delivery, reduce pain, affect muscle contraction, accelerate bone and chronic wound healing have been used medically since 1830 when Carlo Matteucci reported that injured tissue generated an electrical current. In some therapeutic modalities, such as the treatment of Bell’s palsy, a reduction of fine lines and wrinkles, improved skin tone and plumpness have been reported. In the early 1970s skin aestheticians incorporated microcurrent treatment into their product offerings claiming improvement in the appearance of the skin.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.808
      Issue No: Vol. 140, No. 7 (2020)
       
  • 791 Promoting cutaneous wound healing with nutraceutical porcine type I
           collagen peptides
    • Authors: K. Mistry; B. Van Der Steen, B. Vanhoecke, F. van Holthoon, A. Kleinnijenhuis, T. Clifford, M. Labus, P. Lovat, A. McConnell
      Abstract: Type I collagen, the most abundant protein in human skin is integral to cutaneous wound healing, promoting fibroblast proliferation whilst increasing wound tensile strength. Ageing leads to decreased type I collagen synthesis and greater disorganisation within the extracellular matrix resulting in impaired wound healing, an increasing burden in an ageing population. Synthetic type I collagen peptides have been shown to enhance cell migration and proliferation leading to the current hypothesis that nutraceutical porcine type I collagen peptides may enhance dermal collagen levels in aged individuals and promote cutaneous wound healing.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.806
      Issue No: Vol. 140, No. 7 (2020)
       
  • 789 Evolution of an Engrailed 1 enhancer underlies expanded sweat gland
           density of humans
    • Authors: D. Aldea; Y. Atsuta, B. Kokalari, S. Schaffner, Y. Kamberov
      Abstract: In humans, heat dissipation is mainly achieved by the evaporation of sweat. Key to the effectiveness of this thermoregulatory mechanism is the human-specific evolution of a dramatic increase in eccrine sweat gland density. The genetic changes underlying the evolution of this adaptive trait are unknown. In humans, expression of the Engrailed 1 (En1) transcription factor correlates with the onset of sweat gland formation. In mice, regulation of ecotdermal En1 expression is a major determinant of natural variation in gland density between strains and increased En1 promotes the specification of more eccrine glands.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.803
      Issue No: Vol. 140, No. 7 (2020)
       
  • 788 Evidence for epithelial cells in blood and bone marrow of untreated
           murine and human subjects
    • Authors: S. Holtorf; J. Monts, T. Schuster, R. Morris
      Abstract: Cytokeratin positive cells are frequently found in the blood and bone marrow of patients with epithelial cancers and are attributed to metastasis. Interestingly, we observed in a number of publications that unaffected human controls expressed traces of cytokeratin mRNAs. To determine the presence of epithelial cells in normal blood and bone marrow, we used immunofluorescence microscopy (IF), Krt1-14;mTmG transgenic mice, qRT-PCR, and fluorescence activated cell sorting (FACS). We have made several interesting findings.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.802
      Issue No: Vol. 140, No. 7 (2020)
       
  • 786 A master regulator function of hairless in skin homeostasis and immune
           regulation
    • Authors: W. Ha; M. Mihlan, A. Hinde, K. Krotova, G. Aslanidi, L. Xie, R. Ricci, A. Christiano, L. Liu
      Abstract: The hairless (Hr) gene plays an essential role in hair follicle development and skin homeostasis. Both humans and mice lacking hairless activity suffer from complete hair loss and abnormal epidermal proliferation and differentiation, although the mechanisms underlying such developmental defects remain largely unknown. We recently demonstrated that Hr encodes a histone demethylase and acts as an epigenetic regulator of the expression of its target genes. Gene expression profiling studies identified pro-inflammatory IL-1 family cytokines, including IL-36 and IL-1β, among the major targets of Hr.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.800
      Issue No: Vol. 140, No. 7 (2020)
       
  • 785 Phenotypic plasticity of cutaneous squamous cell carcinoma mediated by
           cyclooxygenase-2
    • Authors: H. Moon; D. Kim, L. Donahue, A. White
      Abstract: Murine hair follicle stem cells containing the KrasG12D mutation and p53 loss of function are able to form tumors that represent mesenchymal-like spindle cell morphology. This spindle form of squamous cell carcinomas exhibit hallmarks of epithelial to mesenchymal transition and grow rapidly with invasive characteristics. This mouse model thus provides a method for testing molecular candidates that are critical for a mesenchymal-like phenotype in cutaneous tumors. Cox-2 has been shown to have a role in progression of cutaneous squamous cell carcinomas composed of epithelial-like cells.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.799
      Issue No: Vol. 140, No. 7 (2020)
       
  • 781 IL1α, IL6, and GMCSF are Downstream Mediators of IL17A that Promote
           Asymmetric Stem Cell Self-Renewal in Psoriasis
    • Authors: H. Li; A. Charruyer, T. Weisenberger, A. Khalifa, R. Nguyen, R. Ghadially
      Abstract: Interleukin 17A (IL17A) is a key cytokine in psoriasis and IL17A antibody therapy is an effective treatment for psoriasis. We previously showed that IL17A increases asymmetric stem cell self-renewal divisions in human keratinocytes and plays a role in the hyperproliferation of the epidermis observed in psoriasis. We hypothesized that downstream IL17A-induced keratinocyte cytokines were mediators of the increased asymmetric stem cell self-renewal divisions of psoriasis. We studied cytokines produced by keratinocytes after IL17A treatment and determined their effects on asymmetric stem cell self-renewal divisions in human keratinocytes in vitro, using sister pair analysis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.795
      Issue No: Vol. 140, No. 7 (2020)
       
  • 776 Optimized Vitamin C prodrug for controlled release and antioxidant
           activity
    • Authors: C. Jacques; C. Genies, D. Bacqueville, N. Borotra, M. Noizet, A. Tourette, S. Bessou-Touya, H. Duplan
      Abstract: Vitamin C (vitC) plays key roles in many biological processes and it is a powerful anti-oxidant which protect the skin against UV exposure. Nevertheless, vitC is unstable under oxidative conditions. A pro-vitamin C, Ascorbic acid 2-glucoside (AA2G) was developed to stabilize vitC. The purpose of the study was to assess skin delivery, metabolism and antioxidant effect of AA2G compared to two concurrent products with 5% of encapsulated vitC or with 15% of free vitC. Skin delivery and metabolism studies were performed on human skin explants by UHPLC/UV.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.790
      Issue No: Vol. 140, No. 7 (2020)
       
  • 775 Hyperactivation of sympathetic nerves drives melanocyte stem cell
           depletion
    • Authors: B. Zhang; S. Ma, I. Rachman, M. He, P. Baral, S. Choi, W.A. Gonçalves, Y. Shwartz, E.M. Fast, Y. Su, L.I. Zon, A. Regev, J.D. Buenrostro, T.M. Cunha, I.M. Chiu, D. Fisher, Y. Hsu
      Abstract: Empirical and anecdotal evidence have associated stress with accelerated hair greying (formation of unpigmented hairs), but the scientific evidence linking the two is scant. Here, we report that acute stress leads to hair greying through fast depletion of melanocyte stem cells (MeSCs). Combining adrenalectomy, denervation, chemogenetics, cell ablation, and MeSC-specific adrenergic receptor knockout, we found that stress-induced MeSC loss is independent of immune attack or adrenal stress hormones.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.789
      Issue No: Vol. 140, No. 7 (2020)
       
  • 774 Extreme organization of supra-basal cells allows the building of
           modular feather architectures for adaptable flight
    • Authors: C. Chuong; W. Chang, H. Wu, M. Lei, W. Juan
      Abstract: The skin forms the interface between an organism and its environment. To venture into diverse eco-spaces, animals evolve different integumentary organs to adapt. Here we study how feathers are made from a flat epidermis. A feather is made of two modules: a central shaft (rachis) and the bilateral vane which is made of barb branches. The feather has to be light and strong. In the follicle, feather epidermis first forms an epithelial cylinder with basal cells facing the pulp dermis inside. Then supra-basal cells are partitioned to form the rachis and barbs.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.788
      Issue No: Vol. 140, No. 7 (2020)
       
  • 773 Generation of human hair follicle organoids in vitro and ex vivo by
           co-culture of primary human hair matrix keratinocytes and dermal papilla
           fibroblasts
    • Authors: I. Piccini; K. Bakkar, C. Collin-Djangoné, J. Gherardini, R. Paus, M. Bertolini
      Abstract: Hair transplantation for the management of androgenetic alopecia is often limited by the number of donor HFs available for transplantation. Therefore, HF neogenesis technologies that enlarge the number of donor HFs are needed. Here, we assessed the potential of human primary adult hair matrix keratinocytes (HMx), i.e. the cells interacting with dermal papilla fibroblasts (DPs) in vivo, in generating HF organoids when co-cultured with DP spheroids in vitro and in human skin organ culture, compared to primary human adult epidermal keratinocytes (NHEK).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.787
      Issue No: Vol. 140, No. 7 (2020)
       
  • 772 Terminal arborizations of itch-sensing neurons exhibit large receptive
           
    • Authors: L. Han; Y. Xing, H. Steele, H. Hilley, K. Lawson
      Abstract: Itch sensation is initiated by the activation of a specific subset of sensory neurons that project their peripheral and central axons to the skin and spinal cord respectively. Different subtypes of sensory neurons exhibit distinct terminal arborization subserving their functions. Sensory arborization of itch-sensing neurons has never been observed due to the lack of proper molecular genetic tool. Here we have genetically labeled a small subset of DRG sensory neurons using a newly generated MrgprC11CreER mouse line.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.786
      Issue No: Vol. 140, No. 7 (2020)
       
  • 770 Molecular analysis of atopic dermatitis pathogenesis in NC/NgaTnd mice
    • Authors: C.K. Singh; C.A. Mintie, M.A. Ndiaye, G. Chhabra, N. Ahmad
      Abstract: Atopic dermatitis (AD), a chronic inflammatory disease marked by skin itching and lesions, affects>26 million people in the US. A detailed understanding of the molecular pathogenesis of AD may provide novel means for the management of this debilitating disease. To this end, here we used 2,4-dinitrofluorobenzene (DNFB)-induced NC/NgaTnd mouse model that closely mimics AD in humans. After hair removal, AD was induced in 5-week-old female mice by weekly topical application of 0.15% DNFB to dorsal skin.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.784
      Issue No: Vol. 140, No. 7 (2020)
       
  • 769 Single cell transcriptomics reveals dermal fibroblast heterogeneity
           and a progenitor population that shapes fibroblast heterogeneity
    • Authors: X. Zhang; W. Liu, L. Sun, M. Yin, S. Wu, L. Zhang
      Abstract: Dermal fibroblasts (dFBs) are an essential component of skin; they not only produce and organize the extracellular matrix of the dermis, but also are essential for wound healing, hair growth, fibrosis and defense against infection. Fibroblast heterogeneity has long been recognized in mouse and human skin, but the cellular hierarchy and mechanisms governing fibroblast heterogeneity are incompletely understood. Here, we used single-cell RNA-sequencing to study how cellular heterogeneity of murine skin is turned at the transcriptional level during post-natal periods using single cells isolated from the skin of new born (P1), young (3 weeks) and adult (2 month) mice.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.783
      Issue No: Vol. 140, No. 7 (2020)
       
  • 767 Discovering the signaling pathways underlying mouse Merkel cell
           development using FACS-based single cell RNA-seq
    • Authors: L. Miao; M. Kelly, S. Barkdull, L. Collado, M. Kelley, I. Brownell
      Abstract: Merkel cells (MC) are innervated light touch sensors that comprise less than 0.3% of the mouse epidermis. Terminally differentiated MC are derived from epithelial progenitors and possess both epidermal and neuronal features. Study of MC development provides a model of lineage-specific epithelial differentiation and gives insight into the tumorigenesis of Merkel cell carcinoma (MCC). Using fluorescence-activated cell sorting (FACS)-based single-cell RNA sequencing (scRNAseq) and MC-specific GFP reporter mice, we captured the transcriptome of single MC from embryonic, neonatal, and postnatal skin.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.781
      Issue No: Vol. 140, No. 7 (2020)
       
  • 766 Keratinocyte differentiation is coupled to mechanical cues through the
           LINC complex
    • Authors: A.G. Zieman; R. Stewart, A.E. Zubek, E. Carley, I. Jalilian, M. King, V. Horsley
      Abstract: Skin tissue is resilient to mechanical forces, but also can respond to prolonged mechanical forces. For example, tissue expanders are used to generate more skin for breast reconstruction surgery. At the cellular level, multiple mechanical forces are exerted on epidermal keratinocytes. Progenitor keratinocytes, unlike differentiated keratinocytes, have both cell-cell adhesions and cell-extracellular matrix (ECM) adhesions that keep progenitor keratinocytes attached to the basement membrane. During keratinocyte differentiation, progenitor keratinocytes lose cell-ECM adhesions and detach from the basement membrane, but the exact mechanism of how this occurs is not fully understood.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.780
      Issue No: Vol. 140, No. 7 (2020)
       
  • 764 DNA dioxygenases Tet1/2/3 control hair matrix keratinocyte
           differentiation and hair shaft shape via regulation of hair keratin gene
           expression
    • Authors: G. Chen; Q. Xu, M. Fessing, A. Mardaryev, A. Sharov, G. Xu, V.A. Botchkarev
      Abstract: DNA methylation and subsequent oxidation of 5-methylcytosine into 5-hydroxymethylcytosine (5hmC), catalyzed by the TET1/2/3 family enzymes, are key epigenetic events regulating development, stem cell differentiation and cellular reprogramming in mammals, while genetic ablation of all three Tet genes is lethal. Here, we show that 5hmC modified DNA and Tet1/2/3 proteins show dynamic changes in their abundance in the developing hair follicles (HFs), as well as during the hair cycle. High levels of 5hmC, Tet2 and Tet3 were seen in differentiating hair matrix keratinocytes (KCs), outer and inner root sheaths, hair shaft and dermal papilla.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.778
      Issue No: Vol. 140, No. 7 (2020)
       
  • 763 Exosomes derived from human umbilical cord mesenchymal stem cells
           relieve psoriasis-like skin inflammation
    • Authors: Y. Zhang; J. Yan, Q. Sun
      Abstract: Exosomes are microvesicles secreted from the endosomal membrane and have been shown to act as regulators of cell-cell communication.The immunomodulatory effect of mesenchymal stromal cells (MSCs) is partially mediated by MSC-derived exosomes (MSC-exo).MSC-exo are a very attractive candidate for cell therapy applications in several inflammatory diseases.Psoriasis is a chronic immune-mediated inflammatory skin disease.In recent years,studies have proved that the IL-23/IL-17 axis is closely related to the immunological pathogenesis of psoriasis,and plays a key role in the occurrence and development of psoriasis.Because of the immunomodulatory properties of umbilical cord mesenchymal stem cell-derived exosomes (hucMSCs-Exo),we investigated whether hucMSCs-Exo can ameliorate psoriasis inflammation,and explored the underlying mechanisms.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.777
      Issue No: Vol. 140, No. 7 (2020)
       
  • 757 Clinical efficacy of topical autophagy activator on acne-prone skin
    • Authors: K. Shin; K. Shin, S. Yoon, J. Jung, E. Hwang, H. Chung, S. Lee, S. Jeong
      Abstract: Hyperkeratosis in follicular infundibulum, increased sebum formation, and inflammation are major factors in acne pathogenesis. Recent studies about the important roles of autophagy signaling in sebogenesis and epidermal differentiation suggest potential benefits of autophagy activator on acne. In this study, in vitro and clinical studies were performed to investigate the effects of autophagy activator on acne-prone skin. Autophagy signaling in sebocytes SZ95 and keratinocytes was observed and effects of autophagy activating peptide on sebum generation in sebocytes was also investigated.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.771
      Issue No: Vol. 140, No. 7 (2020)
       
  • 756 Untangling G-protein-coupled receptor signaling and Creb in hair
           follicle homeostasis
    • Authors: M. Miranda; I.P. Avila, J. Esparza, W.E. Lowry
      Abstract: Hair follicle stem cells (HFSCs) located in the stem cell compartment called the bulge have been shown to be the cells responsible for growing hair shafts de novo during each hair cycle, influenced by intrinsic and extrinsic signals among epithelial, mesenchymal, and nervous tissues. The interaction between the sympathetic nervous system and HFSC behavior is largely understudied. Sympathetic nerve innervation of the bulge has been previously shown to be hair-cycle dependent, and adrenoreceptor agonists have been demonstrated to modulate hair growth.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.770
      Issue No: Vol. 140, No. 7 (2020)
       
  • 753 Protecting skin matrisome to prevent skin aging progress
    • Authors: P. Warnault; L. Duriot, A. Salwinski, H. Chajra, M. Frechet
      Abstract: Extracellular matrix (ECM) proteins are major components of human body and precisely human skin. In the skin the ECM is the most abundant in the dermal compartment. The human matrisome has been characterized recently, to clarify the definition of an ECM protein. Matrisome genes were divided in two main groups: the core matrisome and the matrisome-associated genes. While, the core matrisome group is divided in three categories: collagens, proteoglycans and glycoproteins; the matrisome-associated genes were classified as encoding ECM-affiliated proteins, ECM regulators or secreted factors.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.767
      Issue No: Vol. 140, No. 7 (2020)
       
  • 752 Diet and the dermal white adipose tissue: Analyzing shifts in the
           cutaneous lipid landscape in response to dietary change
    • Authors: W. Gao; M. Edwards, B. Thompson, J. McDonald, T.A. Harris-Tryon
      Abstract: The increased prevalence of diet-induced obesity and its associated skin conditions continues to plague modern populations. Though it has been established that diet affects subcutaneous and visceral white adipose, less is known about the impact of the diet on the dermal white adipose tissue — a superficial layer of cutaneous adipose with a variety of metabolic and immune functions. This study sought to investigate the impact of diet on the dermal white adipose tissue (dWAT). Four groups of mice were fed either: 1) a standard diet higher in proteins, 2) a ketogenic diet higher in fat, 3) a western diet higher in fat and carbohydrates, or 4) a tailored steatohepatitis (NASH) diet high in fat, fructose, and cholesterol.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.766
      Issue No: Vol. 140, No. 7 (2020)
       
  • 751 A study of novel skin elasticity index using by CutiScan®
    • Authors: S. Kim; M. Kim, J. Han, E. Kim
      Abstract: Skin is a complex with viscoelastic characteristic, composed of the subcutaneous tissue, the epidermis and dermal matrix. The skin undergoes degenerative changes with aging; reduction of elasticity, epidermal thickness and collagen, elastic fiber, increment of wrinkle, dryness and anisotropy. The CutiScan® provides information not only the viscoelastic properties but also on anisotropy of the skin. There are total 17,640 displacement raw data including time (4seconds) and angle (360 degrees) information and existing elasticity parameter(V3) of this system appears 360 values after measurement.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.765
      Issue No: Vol. 140, No. 7 (2020)
       
  • 746 The study on the role of FOXE1 in the pathogenesis of psoriasis
    • Authors: M. Liu; Y. Zheng
      Abstract: Background: FOXE1 is a DNA-binding protein involved in the regulation of cell growth and differentiation. It has been found that FOXE1 is involved in the pathogenesis of several kinds of cancer. But its specific role and mechanism in psoriasis remain unclear. Objectives: The aim of this study is to investigate the role of FOXE1 in the pathogenesis of psoriasis. Methods: 1. Immunohistochemistry was used to detect the expression of FOXE1 in normal skin tissues and psoriatic lesions; qRT-PCR and Western Blot (WB) were used to determine the expression of FOXE1 in healthy controls and psoriatic lesions, as well as the imiquimod mice models.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.760
      Issue No: Vol. 140, No. 7 (2020)
       
  • 745 Study of clinical and pathological features in 729 cases of nevus
           sebaceous
    • Authors: T. Wang; Q. Li, L. Zhao, S. Geng
      Abstract: Objective: To observe the manifestations and pathological features of NS and analyze the composition of concomitant or secondary diseases in NS. Methods: Collect baseline data of patients with NS and classify their clinical manifestations and review the pathological diagnosis of NS, then analyze the statistical results with SPSS21.0. Results: 1. There were 729 cases of NS, 434 (59.53%) for males and 295 (40.47%) for females, with a male to female ratio of 1.47:1. The average age of surgical excision was 16.16 years.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.759
      Issue No: Vol. 140, No. 7 (2020)
       
  • 744 A study of skin-age analysis method using five parameters and skin
           characteristics of subjects using First Care Activation Serum for
           long-term period
    • Authors: S. Jang; G. Cho, S. Choi, Y. Jung, J. Han, E. Kim
      Abstract: Introduction : Recently, many researches have been actively conducted to evaluate age by artificial intelligence using various parameters. Prediction of skin age is important because biological age and skin age differ due to various environmental factors, beauty habits, and genetic factors. This study predicted skin age by using multiple regression analysis by five skin parameters that affect aging, and analyzed the predicted age of the group using the First Care Activation Serum and the group without it.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.758
      Issue No: Vol. 140, No. 7 (2020)
       
  • 743 Midkine and pleiotrophin are expressed in keloids
    • Authors: V. Cantu; A. Tran, I. Dougherty, D. Glass
      Abstract: Midkine (MDK) is a 15.5kDa heparin-binding growth factor induced by retinoids and inhibited by corticosteroids. It is closely related in structure to another low molecular weight protein, Pleiotrophin (PTN, 18.9kDa). Both MDK and PTN are involved in many important biological pathways including embryogenesis, tumorigenesis, and wound healing. They bind to a number of different receptors, including Protein-Tyrosine Phosphatase, Receptor-Type Zeta-1 (PTZPRZ1), which is known to play a key role in regulating cell growth, differentiation and transformation.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.756
      Issue No: Vol. 140, No. 7 (2020)
       
  • 740 IgG autoantibodies correlate with Hidradenitis Suppurativa clinical
           severity
    • Authors: W.D. Shipman; M.L. Kerns, C. Carmona-Rivera, C. Zhu, Q. Li, L.A. Barnes, M.J. Kaplan, G.A. Okoye, A.S. Byrd
      Abstract: Hidradenitis suppurativa (HS) is a chronic skin disorder with debilitating draining abscesses and sinus tracts in intertriginous areas. Although classically viewed as an autoinflammatory disorder, in part mediated by hyperactive neutrophils, HS is associated with several autoimmune conditions, including inflammatory bowel disease (IBD) and spondyloarthritis (SpA). Key mediators of autoimmunity, high-affinity, somatically mutated IgG autoantibodies (AAbs) reflect a disruption of homeostatic pathways related to cell clearance and antigen-receptor signaling.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.753
      Issue No: Vol. 140, No. 7 (2020)
       
  • 738 Friend or foe: Elevated sera levels of IgM autoantibodies targeting
           hair follicle components detected in patients with Hidradenitis
           Suppurativa
    • Authors: M.L. Kerns; C. Carmona-Rivera, C. Zhu, Q. Li, W.D. Shipman, L.A. Barnes, M.J. Kaplan, G.A. Okoye, A.S. Byrd
      Abstract: Through their recognition of self-antigen, a repertoire of germline encoded IgM antibodies perform the vital housekeeping task of phagocytic clearance of dying cells by the innate immune system. However, pathogenic levels of IgM autoantibodies (AAbs) can recognize neo-epitopes exposed in damaged tissue and initiate uncontrolled inflammation. Hidradenitis suppurativa (HS) is a chronic debilitating disease characterized by recurrent draining nodules and scarring in hair bearing areas of the skin. Hair follicle rupture, due to an unknown stimulus, is postulated to cause an abnormal innate immune response culminating in sterile neutrophil inflammation.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.751
      Issue No: Vol. 140, No. 7 (2020)
       
  • 736 Racial disparities in biologics utilization for psoriasis
    • Authors: W. Hodges; T. Bhat, C. Herbosa, S. Kwatra, A. Musiek, C.M. Mann, Y. Semenov
      Abstract: Biologic therapies are an effective and increasingly common treatment option for moderate to severe psoriasis. However, little is known about biologics utilization in patients of different races. We sought to determine if treatment disparities exist in biologic medication use for psoriasis between different races by analyzing patient records at a large academic medical center. Data was collected using SlicerDicer in the Epic electronic medical record and statistical analyses were performed using SAS Studio version 3.8.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.749
      Issue No: Vol. 140, No. 7 (2020)
       
  • 735 Dermatologists’ assessments and treatment of atopic dermatitis
           differ by patient race
    • Authors: J. Takeshita; A. Sevagamoorthy, A. Bazen, D. Shin, F.K. Barg
      Abstract: Racial/ethnic, gender, and socioeconomic disparities in dermatologic care have been suggested for various skin diseases. The contribution of dermatologists’ practices to such disparities remains unknown. We aimed to evaluate whether dermatologists’ assessments and treatment of atopic dermatitis (AD) differ by patient race, gender, or socioeconomic status (SES). We conducted a cross-sectional survey study of a random sample of dermatologists (N=3,351) who are members of the American Academy of Dermatology.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.748
      Issue No: Vol. 140, No. 7 (2020)
       
  • 733 SFRP2-expressing, COL11A1-expressing fibroblasts are the major
           fibroblast population within keloids
    • Authors: I. Dougherty; V. Cantu, D. Glass
      Abstract: Fibroblasts perform a multitude of processes within skin: extracellular matrix (ECM) production, regulation of inflammation, facilitate cutaneous repair after injury, and serve as pluripotent mesenchymal cells. Recent evidence suggests that there are two main major fibroblast populations in human skin: SFRP2-expressing fibroblasts and FMO1-expressing fibroblasts, and five minor populations, each expressing discrete genes: CRABP1, COL11A1, FMO2, PRG4, or C2ORF40. Keloids are benign fibroproliferative tumors that result from an exaggerated response to cutaneous wound healing.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.746
      Issue No: Vol. 140, No. 7 (2020)
       
  • 732 Clinical efficacy analysis of narrow spectrum intense pulsed light in
           the treatment of acne-related erythema in Chinese patients
    • Authors: Y. Yang; Y. Zheng
      Abstract: Objective: To evaluate the efficacy and safety of Dye narrow-band pulsed light (DPL) in the treatment of Acne Vulgaris in Chinese patients. Methods: The clinical data of 50 patients with erythema after facial acne who had received treatment in the Hospital between January 2019 to June 2019 were analyzed.50 patients with acne-related erythema, aged 15 to 36 years, with a course of 2 to 6 years, with an average of 2.6 years. The following conditions are excluded: (1) a viral infection on the face or the wound incision has not healed; (2) a history of photosensitivity or taking photosensitivity drugs within 4 weeks; (3) patients who have taken oral retinoic acid in the past six months; (4) pregnancy and actating women; (5) patients with heart, kidney, liver insufficiency or other serious underlying diseases; (6) patients with mental illness.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.745
      Issue No: Vol. 140, No. 7 (2020)
       
  • 731 Risk of C. Difficile infection among hidradenitis suppurativa patients
           prescribed prolonged clindamycin course
    • Authors: R. Kwak; A.P. Charrow
      Abstract: Hidradenitis Suppurativa (HS) is a chronic disease with a higher incidence among African American (AA) and Hispanic population in the United States. Prolonged antibiotics (e.g. clindamycin +/- rifampin for 10-12 weeks) are recommended, yet the risk of CDI among HS patients receiving the prolonged course is unknown. We performed a retrospective chart review of all patients at Brigham and Women’s and Massachusetts General Hospitals between 1999 to 2019. We identified 503 patients who were diagnosed with HS, seen by a dermatologist, and treated with clindamycin, of whom 209 were confirmed to have received oral (n=190, 90.9%) and/or intravenous (IV) (n=25, 12.0%) clindamycin for HS.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.744
      Issue No: Vol. 140, No. 7 (2020)
       
  • 730 Salt and pepper dyspigmentation in dermatomyositis with TIF1-γ
           autoantibodies
    • Authors: K. Svigos; D. Gutierrez, A. Femia, N.K. Brinster, K. Lo Sicco
      Abstract: A 48-year-old man with dermatomyositis (DM), significant muscle involvement, and transcription intermediary factor 1-γ (TIF1-γ) autoantibodies was referred to dermatology for widespread areas of dyspigmentation over six months. His rheumatologist treated him with tapering oral glucocorticoids and six infusions of rituximab 1000mg. With this therapy, his pruritic dyspigmentation progressed with only slight improvement of proximal muscle weakness. On physical examination, there were hypopigmented patches with erythema on the face, trunk, and back, notably sparing follicular ostia.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.743
      Issue No: Vol. 140, No. 7 (2020)
       
  • 729 Understanding the intersectional stigma of HIV-related dermatologic
           disorders in Kenya
    • Authors: D. McMahon; L. Butler, N. Busakhala, L. Chemtai, A. Semeere, H. Byakwaga, M. Laker-Oketta, J. Martin, I. Bassett, E. Freeman
      Abstract: Stigma is an independent determinant of health inequities for people with HIV and skin disease, yet few studies have examined how the intersection of these conditions influences overall stigma. This intersectional stigma may be particularly prominent for people with Kaposi's Sarcoma (KS), a common HIV-related dermatologic condition in sub-Saharan Africa. In this study we used qualitative interviews with KS patients in Kenya to assess HIV stigma, dermatologic stigma, and intersectional stigma for both conditions.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.742
      Issue No: Vol. 140, No. 7 (2020)
       
  • 728 Doctor-level multi-classification of skin diseases and a dataset for
           the Yellow Race
    • Authors: X. Chen; S. Zhao, Y. Kuang, M. Chen, W. Zhu
      Abstract: Convolutional Neural Networks has superior performance in image recognition. But it relies on a large amount of data. However, there has not been public skin disease dataset for a specific race. This paper builds such a dataset for the yellow race. It includes 108,248 images from 474 different skin diseases, and part of these images are also annotated with location for individual skin lesions. All these annotations are validated by at least 3 dermatologists and matched with corresponding pathology information as golden standard.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.741
      Issue No: Vol. 140, No. 7 (2020)
       
  • 727 Keloids are associated with Th2, JAK3, and CCR9/CCL25 inflammation
    • Authors: J. Wu; E. Del Duca, M. Espino, A. Diaz, N. Zhang, A. Gontzes, Y. Estrada, J.G. Krueger, A. Pavel, E. Guttman-Yassky
      Abstract: Keloids occur due to disturbed wound healing and abnormal collagen production, often affecting African American (AA) and Asian populations. An increased prevalence of atopic conditions, particularly asthma, has been described in keloid patients. However, its immune pathomechanism has not been studied, inhibiting therapeutic development. To evaluate the inflammatory signature of keloids in skin we obtained lesional and nonlesional biopsies from 3 AA patients with new onset keloids and 5 AA healthy controls.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.740
      Issue No: Vol. 140, No. 7 (2020)
       
  • 724 Patient race affects dermatologists’ assessments and treatment
           of psoriasis
    • Authors: A. Sevagamoorthy; A. Bazen, D. Shin, F.K. Barg, J. Takeshita
      Abstract: Racial disparities in psoriasis treatment have been suggested. Little is known about how physicians’ assessments and treatment patterns may contribute to these disparities. We aimed to evaluate whether patient race, gender, or socioeconomic status affect dermatologists’ assessment and treatment of psoriasis. We conducted a cross-sectional survey study of a random sample of dermatologists (N=3,352) who are members of the American Academy of Dermatology. Each dermatologist was randomly assigned one of eight identical survey options which differed only by either patient race (white vs black), gender (male vs female), or socio-economic status (high vs low).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.737
      Issue No: Vol. 140, No. 7 (2020)
       
  • 723 Racial differences in the health-related quality of life of chronic
           pruritus patients
    • Authors: K. Whang; Y. Semenov, R. Khanna, K. Williams, V. Mahadevan, S. Kwatra
      Abstract: Chronic pruritus is a condition with profound impact on quality of life (QoL), which has been shown to vary with race. The goal of our study was to characterize the racial differences in the health-related QoL of patients diagnosed with chronic pruritus. We administered a cross-sectional survey of 95 patients with chronic pruritus utilizing the Ontario Health Utilities Index Mark 3 (HUI3) questionnaire. We obtained normal population data from healthy US adults (n=4,187) from the 2002-2003 Joint Canada/United States Survey of Health.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.736
      Issue No: Vol. 140, No. 7 (2020)
       
  • 721 Abnormal autophagosome formation increased melanocyte sensitivity to
           H2O2-induced oxidative stress in vitiligo
    • Authors: T. Cui; S. Li, K. Li, Z. Jian, C. Li
      Abstract: Autophagy is a controlled self-digestion process which can protect cells against oxidative damage. Dysregulated autophagy has been demonstrated to increase melanocyte sensitivity to oxidative stress in vitiligo. However, the exact mechanism is still not clear. This study aimed to determine the implications of autophagy for melanocyte survival in response to oxidative stress. Our results demonstrated that the autophagic flux in PIG1 exposure to H2O2 was significantly enhanced compared with that in PIG3V, which were accompanied by high level of ROS accumulation, membrane potential changes, and increased apoptosis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.734
      Issue No: Vol. 140, No. 7 (2020)
       
  • 719 A new free teledermatology platform effectively delivers specialist
           recommendations for uninsured Latino patients
    • Authors: N. Rodriguez; C.L. Simpson
      Abstract: Access to specialist medical care in the U.S. is limited for economically disadvantaged patients and this worsens healthcare disparities for minority populations. To address this issue, the American Academy of Dermatology launched a new free teledermatology platform to deliver specialist advice to primary care providers (PCPs) in under-resourced clinics. Using the smartphone-based program, we offered consultations to PCPs in an urban community health clinic serving uninsured patients with a focus on Latino immigrants, who face various barriers to care.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.732
      Issue No: Vol. 140, No. 7 (2020)
       
  • 717 Enhanced molecular signatures in cutaneous lupus erythematosus
           patients support distinct pathogenic pathways in African American patients
           
    • Authors: J.L. Zhu; L. Tran, F. Zheng, J. James, J. Guthridge, B.F. Chong
      Abstract: Cutaneous lupus erythematosus (CLE) is an autoimmune disease with clinical sequelae such as skin damage that disproportionately affects African Americans (AAs). Recently, a novel approach in gene expression analyses was devised that groups genes into transcriptional modules (i.e. apoptosis, protein synthesis, inflammation) to identify relevant gene signatures. This technique helped identify a unique interferon signature in systemic lupus patients. Thus, we applied this modular analysis approach to identify molecular signatures unique to AA CLE patients.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.730
      Issue No: Vol. 140, No. 7 (2020)
       
  • 716 Supporting diversity in skin science: Development of a summer
           curriculum for underrepresented minority medical students
    • Authors: M. Paul; J. Lester, R. Ricardo-Gonzalez, H. Naik
      Abstract: Diversity in the scientific workforce fosters innovation, improves research quality, increases the likelihood of generalizable findings, and builds public trust. Despite a recent commitment to diversity in dermatology, gender, racial and ethnic diversity in skin science is still lacking. We developed a 6-week summer curriculum for underrepresented minority (URM) medical students conducting research with a faculty mentor in our department. The curriculum goals were to: create a supportive environment for URM students to develop research mentorship relationships, expose URM students to the breadth of research in dermatology, and facilitate acquisition of skills needed for academic success.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.729
      Issue No: Vol. 140, No. 7 (2020)
       
  • 715 Expectations of care among African-American Women with hair loss: A
           cross-sectional study
    • Authors: B. De Souza; L. Burns, D. Hagigeorges, E. Flynn, M. Senna
      Abstract: Despite being the fourth most common diagnosis among African-Americans presenting to the dermatologist, alopecia is often self-managed by African-American women (AAW) with only 23% seeking medical care. In this study, we aim to capture patient-reported healthcare utilization for alopecia, related treatments, and barriers to alopecia care among AAW with hair disorders. This cross-sectional study utilized REDCap to distribute a survey to AAW hair groups and profiles on social media in winter 2019.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.728
      Issue No: Vol. 140, No. 7 (2020)
       
  • 713 Do race and ethnicity impact healthcare utilization and costs' A
           population study among U.S. non-melanoma skin cancer patients
    • Authors: T. Sierro; L. Blumenthal, J. Hekmatjah, V.S. Chat, C. Read, A. Kassardjian, A.W. Armstrong
      Abstract: Racial and ethnic differences in healthcare utilization and expenditures for non-melanoma skin cancer (NMSC) patients are unknown. Compared to white patients, NMSC is less prevalent in other racial groups. However, it is important to evaluate healthcare use and costs among racial and ethnic minorities with NMSC to identify gaps in care. This study aims to identify and compare healthcare expenditures and utilization among non-Hispanic white, non-Hispanic black, and Hispanic white patients with NMSC.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.726
      Issue No: Vol. 140, No. 7 (2020)
       
  • 712 Using scanning electron microscopy to elucidate the role of hair shaft
           malformation in the pathogenesis of Central Centrifugal Cicatricial
           Alopecia
    • Authors: E.F. Araoye; N. Dlova, C. Aguh
      Abstract: Central Centrifugal Cicatricial Alopecia (CCCA) is a scarring alopecia most common in black women. Its presentation follows a seemingly genetic pattern affecting clusters of women within a family. A study that aimed to elucidate the genetic basis for CCCA found an increased incidence of mutations and decreased expression of PID3, a gene that encodes for peptidyl arginine deiminase type 3, essential for the formation of a normal hair shaft, in scalp samples of patients with CCCA (1). Of note, PID3 is one of three genes implicated in uncombable hair syndrome, a disorder where the hair shaft appears triangular or heart shaped in cross section when viewed under scanning electron microscopy (SEM) (2).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.725
      Issue No: Vol. 140, No. 7 (2020)
       
  • 710 Vitiligo clinical and lesional molecular features associated with
           favorable response to NBUVB combined with topical tacrolimus
    • Authors: Q. Yang; J. Xu, M. Su, G. Zhang, X. Zhang, H. Lui, P. Zhou, Y. Zhou
      Abstract: Vitiligo is an autoimmune disease resulting in death of melanocytes, causing disfiguring loss of skin pigmentation. It is known that numerous differentially expressed genes (DEGs) are present in vitiligo lesional skin. However, it is unknown if vitiligo DEGs are correlated with meaningful clinical characteristics, such as disease severity, duration and therapeutic response. The purpose of this study is to evaluate the clinical correlates of vitiligo DEGs. Thirty seven vitiligo patients were recruited for this study, each consented to skin biopsies from lesional as well as non-lesional skin.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.722
      Issue No: Vol. 140, No. 7 (2020)
       
  • 709 Malassezin: A preclinical assessment of a novel microbiome-based skin
           lightener
    • Authors: E.T. McCraw; E. Coryell, M. Duvvuri, P. Grimes, M. Einziger, A. Einziger, T. Macdonald, J. Sackier
      Abstract: The Malassezia species are commensal to the skin microbiome and implicated in skin and scalp diseases, including tinea versicolor and seborrheic dermatitis. In cases of tinea versicolor, Malassezia overgrowth often results in skin pigmentation changes (including both hypo- and hyper-pigmentation). The purpose of this study was to link the benign pigmentation change to malassezin, an indole metabolite secreted by Malassezia furfur, and demonstrate the safety and ability of malassezin to decrease melanin in in-vitro models.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.721
      Issue No: Vol. 140, No. 7 (2020)
       
  • 707 MITF-mediated changes of tumor architecture, tensile stress and
           extracellular matrix control intratumor heterogeneity in melanoma
    • Authors: L. Spoerri; C.A. Tonnessen-Murray, K.A. Beaumont, D. Hill, R. Jurek, G. Gunasingh, W. Weninger, N.K. Haass
      Abstract: Dynamic heterogeneity is a prime source for drug resistance, and understanding its underlying mechanism is crucial to design effective therapies. Using real-time cell cycle imaging (FUCCI) and single-plane illumination microscopy (SPIM), we demonstrate dynamic heterogeneity in melanoma spheroids and xenografts. This heterogeneity was characterized by the presence of clusters of proliferating cells and clusters of G1-arrested cells in the same spheroid or xenograft. The location of the quiescent zones suggested oxygen/nutrient deprivation as the cause of cell cycle arrest, and the G1-arrested cells reversed to cycling when re-cultured under normoxia in 2D culture.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.719
      Issue No: Vol. 140, No. 7 (2020)
       
  • 706 Facility type and location impact survival of spindle cell melanoma
    • Authors: S. Behbahani; K.M. Karanfilian, W.C. Lambert
      Abstract: Spindle cell melanoma (SCM) is a rare histological subtype of malignant melanoma composed of spindled neoplastic cells. Due to its rarity, population-based clinical and epidemiological characterization is necessary. We searched the National Cancer Database (NCDB) for all cases of SCM confirmed histologically diagnosed from 2004-2016 and excluded those with missing survival information (n=3906). Kaplan-Meier (KM) and Cox proportional-hazards models were used to analyze the epidemiology and overall survival (OS) of SCM.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.718
      Issue No: Vol. 140, No. 7 (2020)
       
  • 705 Bioluminescent identification of a novel EMT-directed experimental
           therapeutic blocking invasion and metastasis in human malignant melanoma
    • Authors: J. Jandova; G.T. Wondrak
      Abstract: Epithelial-mesenchymal transition (EMT) is a key molecular pathway and promising therapeutic target in human melanomagenesis. Employing a phenotypic screen that examines therapeutic suppression of EMT in A375 malignant melanoma cells, we have now identified a drug-like chemical entity, 4-[(7-chloro-2-methoxy-1,5-dihydrobenzo[b] [1,5]naphthyridin-10-yl)imino]-2,6-bis(pyrrolidin-ylmethyl)cyclohexa-2,5-dien 1-one (PYD), as a potent EMT inhibitor. In a SCID mouse metastasis model employing time-resolved bioluminescent detection of lung tumorigenesis after tail-vain injection of stable A375-luciferase transfectants (monitored over three weeks post-injection), lung tumor burden imposed by A375-luc2 cells was attenuated by PYD-based systemic intervention (100 mg/kg; p.o., q.d., 3 d regimen), a curative outcome achievable without causation of any organ toxicity or negative impact on body weight.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.717
      Issue No: Vol. 140, No. 7 (2020)
       
  • 703 Laser Capture Microdissection and genetic analysis identify dysplastic
           nevi as a subgroup of common nevi rather than a progressive state towards
           melanoma
    • Authors: K. Navrazhina; J.W. Frew, N. Gulati, D. Grand, S. Garcet, H. Mitsui, J.G. Krueger
      Abstract: Whether the dysplastic nevus (DN) is a premalignant lesion serving as a precursor to malignant melanoma (MM), or a subset of the common melanocytic nevus (CN) has remained a source of controversy. We employed Laser Capture Microdissection (LCM) in combination with microarray analysis to identify melanocyte gene expression signatures specific to DNs. Excisional biopsies of CNs, DNs and primary MM were obtained. LCM was performed on frozen sections to isolate melanocytes followed by cDNA microarray analysis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.715
      Issue No: Vol. 140, No. 7 (2020)
       
  • 702 Malassezin: A proof of concept study documenting the efficacy of a
           novel microbiome-based ingredient for facial hyperpigmentation
    • Authors: P. Grimes; E.T. McCraw, A. Einziger, M. Einziger
      Abstract: Malassezin is a natural indole compound produced by the fungus Malassezia furfur which causes Tinea Versicolor characterized by patches of hypo and/or hyperpigmentation. Our preliminary in-vitro and ex-vivo experiments documented the ability of Malassezin to decrease skin pigmentation. The objective of this randomized, double blind, controlled study was to investigate the skin lightening effects of novel formulations of Malassezin for facial hyperpigmentation. This 22-week study enrolled subjects with melasma(n=8) and dyschromia(n=12) caused by photodamage.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.714
      Issue No: Vol. 140, No. 7 (2020)
       
  • 701 Dysplastic nevi (DN) patients have high DC-HIL-expressing
           myeloid-derived suppressor cells (MDSC) that may confer increased risk for
           melanoma
    • Authors: J. Chung; V. Ramani, P. Cruz, S. Savory, K. Ariizumi
      Abstract: MDSC are the most potent suppressors of T-cell function due to expression of the DC-HIL receptor. We showed degree of DC-HIL+MDSC proliferation in blood of melanoma patients to correlate with worsening cancer stage but also found DN patients to display a wide range of this index. In FACS analysis, DC-HIL+MDSC/PBMC in DN cases ranged from 0.2 to 4.6% DC-HIL+MDSC/PBMC with a median of 2%. This was significantly higher than DN-free and age-matched healthy donors (HD) whose median index was 0.1 ± 0.1% and of melanoma in-situ (MIS) at 1.1 ± 0.6%, but lower than stage 3 melanoma at 2.2 ± 0.9%.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.713
      Issue No: Vol. 140, No. 7 (2020)
       
  • 699 Gene profiling of acral melanomas
    • Authors: P. Valencia Pena; J. Li
      Abstract: Acral melanoma is a subtype of melanoma that occurs most often in dark skinned individuals. Different from the melanoma subtypes occurring in sun-exposed areas in white patients, dark skinned patients most often present lesions in palms, soles, nails and heels and in advanced stages. Even though advances in genomics and molecular technologies are refining our understanding of biological mechanism of melanoma, most of the studies have been done in non-acral melanomas in white individuals. Those studies have led to identifying common mutations or alterations in genes of BRAF, NRAS and KIT.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.711
      Issue No: Vol. 140, No. 7 (2020)
       
  • 696 MAPK inhibition synergizes with TNF-alpha to promote the expression of
           STAT1, IRF-1 and MHC class I
    • Authors: B.P. Pollack; Y. Chang
      Abstract: Molecular events that activate the MAPK signaling pathway are key events in melanoma pathogenesis and MAPK inhibitors (MAPKIs) such as those targeting BRAFV600E and MEK1/2 are currently in use to treat melanoma patients. In addition to their effects on tumor cell proliferation and survival, MAPKIs can increase T cell infiltration into tumors and MHC expression and clinical trials combining MAPKIs with immune-based therapies are currently being performed. TNF-alpha is a pleiotropic cytokine that plays a complex role in melanoma and has been shown to block apoptosis induced by MAPKIs.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.708
      Issue No: Vol. 140, No. 7 (2020)
       
  • 694 An epidermal model containing melanocytes for skin pigmentation and
           lightening studies
    • Authors: M. Klausner; B. Breyfogle, A. Armento, J. Oldach, S. Ayehunie
      Abstract: Considerable interest exists in evaluating raw materials and/or skin care formulations which cause lightening of the skin. These products are utilized to modulate one’s natural skin color or to combat skin pigmentation disorders such as melasma, dark spots, solar lentigo, and other hyperpigmentation lesions. To aid in the development and testing of such products, we have developed a skin whitening protocol using the epidermal skin model, MelanoDerm™, to evaluate both raw materials and skin lightening formulations.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.706
      Issue No: Vol. 140, No. 7 (2020)
       
  • 693 p38 signaling regulates human cutaneous metastatic melanoma (MM)
           invasion and MM-dependent disruption of keratinocyte differentiation
    • Authors: A. Kiss; C. Wei, Z. Aligabi, M. Barlas, E. Murphy, F. Glass, A. Friedman, T. Efimova
      Abstract: Advancing our understanding of MM invasion mechanisms is vital for developing new mechanism-based therapies and improving MM outcomes. Here we describe an optimized organotypic human skin equivalent co-culture system of primary epidermal keratinocytes, MM cells, and stromal fibroblasts recently developed in our laboratory in order to reliably model early invasive behavior of MM as well as melanoma-keratinocyte crosstalk in the tissue microenvironment that more accurately reflects human disease pathology.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.705
      Issue No: Vol. 140, No. 7 (2020)
       
  • 692 Using a novel p300 HAT inhibitor as epigenetic therapy to treat BRAF
           inhibitor-resistant melanoma cells
    • Authors: A. Hanly; F. Gibson, K. Kuang, E. Kim, B.E. Zucconi, M. Wu, P. Cole, R. Alani
      Abstract: Despite initial response to targeted therapy in many patients with advanced melanoma, innate and acquired resistance to BRAF inhibitor treatment limits the long-term success of monotherapy with these agents. Reactivation of the mitogen activated protein kinase (MAPK) pathway in melanoma cells is a crucial mechanism in the development of resistance to targeted therapy and usually does not occur through new genetic mutations. Therefore, epigenetic alterations such as histone modifications have emerged as key mediators of the ability of melanoma cells to achieve resistance.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.704
      Issue No: Vol. 140, No. 7 (2020)
       
  • 691 Identification of RNA biomarker candidates in melanocytic tumors using
           digital spatial profiling
    • Authors: M. Kiuru; M. Kriner, G. Zhu, J. Terrell, M. Hoang, J. Beechem, J. McPherson
      Abstract: Early diagnosis of melanoma is imperative for improved prognosis, but current diagnostic methods are based on histological examination and can be inaccurate for a subset of tumors. Additionally, biomarkers of early melanomagenesis and the role of the microenvironment are poorly defined. To identify novel biomarkers, we measured RNA expression of>1,000 cancer- and immune-related genes with spatial resolution in 200μm-diameter regions of interest (ROIs) from patient-derived formalin-fixed, paraffin-embedded (FFPE) tissue sections of common melanocytic nevi, dysplastic nevi, melanoma in situ, and melanoma using the NanoString GeoMX™ Digital Spatial Profiler (DSP).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.703
      Issue No: Vol. 140, No. 7 (2020)
       
  • 690 Re-evaluation of analogues of a potent tyrosinase inhibitor
    • Authors: J. Jerome; T. Mammone
      Abstract: Human skin color is partly determined by the amount and type of melanin, a light-absorbing molecule produced by melanocytes, specialized pigment producing cells in the skin. A key step in the synthesis of melanin in skin is the conversion of tyrosine to DOPAquinone by the enzyme tyrosinase. Multiple approaches exist for inhibiting melanin biosynthesis, many of which target tyrosinase. Tyrosinase is the rate limiting enzyme in the reactions leading to the production of the two melanin pigments, eumelanin and pheomelanin.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.702
      Issue No: Vol. 140, No. 7 (2020)
       
  • 689 Anti-tumor effects and mechanism of 4′-bromo-resveratrol in a
           BRAFV600E/ PTENNULL melanoma mouse model
    • Authors: G. Chhabra; C.K. Singh, M.A. Ndiaye, N. Ahmad
      Abstract: New therapeutic modalities are required for an effective and recurrence-free lasting treatment of metastatic melanoma, which is one of the deadliest forms of cutaneous malignancy. 4’-Bromo-resveratrol (4BR), is a dual small molecule inhibitor of Sirtuin-1 and -3, both of which play tumor-promoting roles in melanoma. Earlier, we showed that 4BR imparted anti-proliferative effects against human melanoma cells in vitro. Here, we determined the in vivo efficacy and mechanism of 4BR against melanoma in a genetically engineered BRAFV600E/PTENNull mouse model that recapitulates human disease with lymph nodes and lung metastases.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.701
      Issue No: Vol. 140, No. 7 (2020)
       
  • 688 Keratinocyte behaviour in normal appearing vitiligo skin
    • Authors: D. Kovacs; E. Bastonini, P. Iacovelli, A. Pacifico, C. Cota, M. Picardo
      Abstract: Cytoarchitectonic and functional interactions among epidermal and dermal compartments are known to regulate melanocyte behaviour and survival. The presence of a deregulated microenvironment able to affect melanocyte homeostasis has been described in both hyper- and hypo-pigmentary disorders. In vitiligo, several data indicate a structural and functional impairment not only of melanocytes, but also of the other cutaneous cells, which appears broadly extended, even in apparently normal skin. In line with these remarks, our group has recently reported some modifications in non lesional fibroblasts, which show a myofibroblast phenotype associated to signs of premature senescence.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.700
      Issue No: Vol. 140, No. 7 (2020)
       
  • 687 Immunoprevention of chemical melanomagenesis through early recognition
           of oncogene mutations
    • Authors: K. Yang; M. Sherwani, B.N. McDaniel, A. Stephens, N. Yusuf, C. Elmets
      Abstract: Melanoma, an aggressive malignancy of melanocytes, is responsible for more deaths than any other skin cancer. We have developed an in vivo murine model of melanomagenesis in which benign pigmented lesions progress to become invasive melanomas. The polyaromatic hydrocarbon DMBA (7,12-dimethylbenz(a)anthracene) was applied to the skin of C3H/HeN mice followed by repeated exposure to phorbol 12-myristate 13-acetate (TPA). This treatment produces a specific activating mutation in the H-ras oncogene, resulting in a single amino acid substitution (leucine for glutamine) at the 61st residue.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.699
      Issue No: Vol. 140, No. 7 (2020)
       
  • 685 Targeted degradation of CD147 proteins in melanoma
    • Authors: Z. Zhou; J. Long, X. Chen, C. Peng
      Abstract: CD147, a transmembrane glycoprotein highly expressed on melanoma cells, plays a vital role in tumor proliferation, invasion, metastasis, thus may be a potential drug target for melanoma. Theoretically, degradation of a target oncogenic protein in cancer cells is superior to inhibition of the same protein in efficacy. PROTACs (proteolysis targeting chimeras) are chimeras connected via a linker which could simultaneously bind to a target protein and an E3 ligase, thereby leading to ubiquitination and subsequent degradation of the target by the ubiquitination system.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.697
      Issue No: Vol. 140, No. 7 (2020)
       
  • 682 A systems biology approach for skin brightening, including autophagy
           as a critical mechanism to control pigmentation
    • Authors: K. Dong; D. Layman, N. Pernodet
      Abstract: We use a lens of systems biology to develop our high-performance skincare products. When targeting brightening, the importance of addressing the pigmentation machinery (tyrosinase inhibition, melanin transfer) is well established. We have, however, identified autophagy, which has recently been shown as another pathway critical not only to pigmentation, but also to inflammation as an important target as well. Autophagy, a cellular degradative and recycling pathway, is essential for cell efficiency, homeostasis, and longevity.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.694
      Issue No: Vol. 140, No. 7 (2020)
       
  • 681 PM2.5 is an AhR agonist that upregulates melanogenesis in human
           melanoma cells A375
    • Authors: F. Peng; Z. Chen, M. Furue, J. Zhang
      Abstract: To investigate the effects of PM2.5 on A375 human melanoma cells and the possible mechanism. The PM2.5of winter haze was collected in Beijing. A375 was treated with different concentrations of PM2.5 suspensions. The morphology of A375 cells was observed by microscopy. The number of viable A375 decreased and the cells lost their normal shape gradually with the treatment of PM2.5. PM2.5 also inhibits the A375 cells viability and induces blockage of S phase.The protein expression of CDk1 and CyclinE1 were significantly decreased after PM2.5 treatment.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.693
      Issue No: Vol. 140, No. 7 (2020)
       
  • 680 IL6/stat3 pathway drives MMP9 to promote invasion in melanoma
    • Authors: L. Lu; X. Chen, J. Su
      Abstract: Interleukin-6 particularly plays a critical role in number of biological events. Different cancer cells, cancer-associated cells and resistant cancer cells overexpress and secrete IL-6 in tumor microenvironment. While, IL6 which was originally identified as a B-cell differentiation factor, is a multifunctional cytokine that regulates the immune response, the acute phase response and inflammation. There are increasing evidences showed that IL-6 can initial promoting anti-tumor immunity to inhibit cancer progress.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.692
      Issue No: Vol. 140, No. 7 (2020)
       
  • 677 Sestrin2 contributes to vemurafenib resistance in braf mutant
           metastatic melanoma cells by detoxifying intracellular reactive oxygen
           species
    • Authors: G. Zhu; S. Wang, T. Gao, C. Li
      Abstract: Melanoma is the most life-threatening skin cancer, accounting for 75% of skin cancer-associated death. Vemurafenib was approved by FDA for the treatment of late-stage melanoma patients with BRAF mutation. However, clinically, most cases developed resistance to it. Hereby we conduct a study to explore the possible role of a stress responsive protein, Sestrin2, in vemurafenib resistance. Previously, we have demonstrated Sestrin2 has a protective role in melanoma cells in response to anoikis via detoxifying intracellular reactive oxygen species (ROS).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.689
      Issue No: Vol. 140, No. 7 (2020)
       
  • 676 Relationship between the physical properties of skin with pigmented
           spots and amount of desmoglein 1 in the stratum corneum
    • Authors: R. Maruyama; A. Kajiwara, A. Tada
      Abstract: The stratum corneum (SC) of the skin is thicker in areas with pigmented spots than in areas without pigmented spots. Despite this difference in SC characteristics, the physical properties of skin with pigmented spots have not yet been studied. We compared skin hardness and measured the amount of desmoglein 1 (Dsg1) in the SC at sites with and without pigmented spots. Since cell adhesion proteins can increase SC thickness, we assumed that the physical properties of the SC are also associated with cell-cell adhesion.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.688
      Issue No: Vol. 140, No. 7 (2020)
       
  • 675 The phosphorylation of CD147 by Fyn plays a critical role in melanoma
           cell growth and metastasis
    • Authors: X. Zhang; Y. Guo, X. Chen, C. Peng
      Abstract: CD147, also known as extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that is highly expressed in tumor cells, particularly melanoma cells, and plays critical roles in tumor cell metastasis through the regulation of matrix metalloprotease (MMP) expression. Studies have demonstrated that CD147 could promote melanoma progression through post-translational modification, such as glycosylation. However, the role of phosphorylation of CD147 in melanoma remains unclear.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.687
      Issue No: Vol. 140, No. 7 (2020)
       
  • 674 The effective killing of the difficult-to-treat melanomas with the
           combinations of MCL1 inhibitors S63845/MIK665 plus Navitoclax
    • Authors: N. Mukherjee; J. Skees, K. Todd, D. West, K. Lambert, W. Robinson, C. Amato, K. Couts, R. Van Gullick, M. MacBeth, K. Nassar, A. Tan, Z. Zhai, M. Fujita, S. Bagby, D. Norris, Y. Shellman
      Abstract: Despite the advancement of melanoma care, a subset of melanoma patients do not respond or relapse with current targeted or immuno-therapies. Therefore, it is necessary to explore other biological processes can be therapeutic. The BCL-2 family of proteins contributes to a cancer cell’s resistance to treatment, and BH3 mimetics drugs that target BCL-2 are effective in hematological cancers. We used genetic knockdown and BH3 mimetics therapeutics to target BCL-2’s anti-apoptotic defenses, and identified MCL1 and BCLXL as crucial melanoma pro-survival members.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.686
      Issue No: Vol. 140, No. 7 (2020)
       
  • 673 Semi-quantitative 5-hmC expression by immunohistochemistry is a useful
           adjunctive technique to help distinguish spitz nevi from spitzoid
           malignant melanomas
    • Authors: K. Bell; M. Tahir, R. Lazova, N. Rodic
      Abstract: The differentiation between Spitz nevi (SN), Atypical Spitzoid neoplasms (ASN) and Spitzoid malignant melanomas (SMM) represents a challenge to dermatopathologists. ASN are lesions in the gray zone for which a definitive histopathologic diagnosis of benign or malignant cannot be made with absolute certainty. We sought to investigate whether the major epigenetic biomarker, 5-hydroxymethylcytosine (5-hmC), is differentially expressed and can aid in the diagnosis of benign and malignant Spitzoid neoplasms.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.685
      Issue No: Vol. 140, No. 7 (2020)
       
  • 672 Targeting lncRNAs AC and BX reveals their critical role in melanoma
           and allows to identify novel phosphor-catalytic vulnerabilities
    • Authors: V. Feichtenschlager; C. Emily, M. Vujic, I. Vujic, C. Chen, A. Lee, S. Ortiz-Urda
      Abstract: More than 80% percent of melanomas harbor mutations in either NRAS or BRAF. Our goal was to identify targets that are key in the process of melanocyte tumorigenesis and molecules that can be used to prevent this event. We have identified two long non-coding RNA (lncRNA) transcripts AC and BX key in the process of melanocyte tumorigenesis and melanoma progression. Tested in a variety of BRAF and NRAS mutated, drug resistant melanoma cell lines and other NRAS cancers such as lung ca and neuroblastoma, RNAi and antisense oligonucleotide (ASO) mediated knockdown of AC and BX lead to a vast reduction of tumor growth in vivo.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.684
      Issue No: Vol. 140, No. 7 (2020)
       
  • 669 CD147 promotes tumorigenesis in squamous cutaneous cancer through
           recruiting MDSC into epidermis through CXCL1/2
    • Authors: Y. Guo; X. Zhang, X. Chen, C. Peng
      Abstract: CD147, a transmembrane glycoprotein belongs to the immunoglobulin superfamily, was highly expressed in various cancers. Recent studies demonstrated that CD147 promoted tumor progression by regulating inflammatory factors and chemokines. Our previous studies found CD147 promoted cutaneous squamous-cell carcinoma (cSCC) through inducing EGFR endocytosis. However, whether CD147 could regulate cSCC by influencing immune cells remains unclear. In this study, we found spontaneous tumors in CD147 epidermal over-expressing transgenic mice (TgCD147-Epi).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.681
      Issue No: Vol. 140, No. 7 (2020)
       
  • 668 Ulcerated melanomas exhibit epigenetic changes in epidermal and immune
           response genes
    • Authors: G. Micevic; M. Bosenberg
      Abstract: The purpose of this study is to identify the genetic and epigenetic changes associated with melanoma ulceration. Clinically, ulceration is consistently associated with shorter disease-free and shorter overall melanoma-specific survival. Ulcerated melanomas have also been associated with a higher risk of melanoma recurrence. However, the molecular changes associated with ulceration are largely unknown. In this study, we examined the differences in DNA methylation patterns between ulcerated and nonulcerated melanoma samples.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.680
      Issue No: Vol. 140, No. 7 (2020)
       
  • 667 β3-tubulin knockdown interferes with microtubule dynamics, cell-cycle
           regulation, and microvesicle release in human melanoma cells
    • Authors: M. Altonsy; P. Surmanowicz, A. Ganguly, G. Lauzon, R. Mydlarski
      Abstract: Introduction: Microvesicles (MV), ranging in size from 100 nm to 1000 nm, play an important role in carcinogenesis by promoting angiogenesis and tumor metastasis, interfering with anti-tumor immunity, and inducing multidrug resistance. The release of MVs requires structural changes in microfilaments, intermediate filaments, and microtubules. Class III β-tubulin (β3-tubulin), one of seven β-tubulin isotypes, is a microtubule component involved in malignant transformation and cancer development. Herein, we characterize the effects of β3-tubulin knockdown on microtubule dynamics, cell cycle regulation, and microvesicle formation in human melanoma cells.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.679
      Issue No: Vol. 140, No. 7 (2020)
       
  • 665 Associations of BIRC2/3/5 copy number gains with clinicopathological
           features of acral melanomas in Taiwan
    • Authors: C. Chu; Y. Sheen
      Abstract: Anti-apoptotic molecules can protect melanoma cells from apoptosis, thus enhancing their aggression. One of the factors responsible for the difficulties in engaging the apoptotic cascade efficiently in melanoma is the up-regulation of conserved inhibitor of apoptosis proteins (IAPs). All IAPs share up to three conserved zinc-binding baculoviral IAP repeats domains. The best described members are cIAP1, cIAP2, X-linked IAP, and surviving, which are encoded by Baculovirus inhibitor of apoptosis repeat containing genes (BIRC2, BIRC3, BIRC4, and BIRC5).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.677
      Issue No: Vol. 140, No. 7 (2020)
       
  • 664 Oncogenic melanocyte stem cells, driven by regenerative niche signals,
           give rise to heterogeneous melanoma resembling human melanoma
    • Authors: Q. Sun; I. Katehis, W. Lee, Y. Mohri, M. Takeo, C. Lim, X. Xu, P.S. Myung, R. Atit, M. Taketo, R. Moubarak, M. Schober, I. Osman, D. Gay, D. Saur, E.K. Nishimura, M. Ito
      Abstract: Melanoma, the most lethal form of skin cancer, is rarely curable at its advanced stages. The early events of this disease, during which treatment would be beneficial, remain poorly elucidated. Melanocyte stem cells (McSCs) residing in the hair follicle niche were proposed to be cells-of-origin for melanoma. To understand the cellular and molecular mechanisms regulating the initiation and progression of McSC-derived melanoma, we established a novel c-Kit-CreER-driven melanoma mouse model that enabled us to specifically target McSCs and trace their oncogenic behaviors.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.676
      Issue No: Vol. 140, No. 7 (2020)
       
  • 662 Targeting the α9-nAChR/PD-L1 axis in antiproliferative effects of
           Allium cepa L. var. proliferum Regel extract on melanoma cells
    • Authors: H. Nguyen; Y. Ho
      Abstract: Programmed cell death ligand-1 (PD-L1) is critical for melanoma development, progression and treatment. The α9 nicotinic acetylcholine receptor (nAChR) is shown to affect melanoma cell proliferation. Allium cepa L. var. proliferum Regel extract possesses anticancer properties. However, the mechanism of Allium cepa L. var. proliferum Regel extract in the antiproliferative effects of melanoma cells remains unclear. Using UPLC-ESI-MS/MS method, a total of 42 compounds were identified in Allium cepa L.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.674
      Issue No: Vol. 140, No. 7 (2020)
       
  • 661 A20 determines the therapeutic effect of anti-PD-1 immunotherapy in
           melanoma
    • Authors: W. Guo; J. Ma, X. Yi, T. Gao, C. Li
      Abstract: The therapeutic effect of immune checkpoint blockade (ICB) therapy, especially the inhibition of programmed cell death protein 1 (PD-1) and its ligand PD-L1, has been verified in melanoma treatment. However, the dissatisfied response rate and therapeutic efficacy of anti-PD-1/PD-L1 therapy remains a major challenge for melanoma treatment. Here, we reported A20 as a critical regulator that determines the therapeutic effect of anti-PD-1 immunotherapy in melanoma. Through the un-targeted MS-based proteomic analysis, we first found that high expression of A20 was significantly associated with therapeutic resistance to anti-PD-1 immunotherapy in melanoma patients.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.673
      Issue No: Vol. 140, No. 7 (2020)
       
  • 660 Dual HDAC and LSD1 inhibition as a novel strategy to overcome BRAF
           inhibitor resistance
    • Authors: F. Gibson; A. Hanly, K. Kuang, J. Kalin, M. Wu, P. Cole, R. Alani
      Abstract: Despite recent advances in the development of targeted and immunotherapies for metastatic melanoma, the majority of tumors demonstrate intrinsic or acquired therapeutic resistance. While the mechanisms of therapeutic resistance in melanoma are varied and often undefined, epigenetic regulation of gene expression and transcriptional programming may play a pivotal role. The potential role of epigenetic changes in the development of resistance to therapies in melanoma and other cancers has led to the development of numerous epigenetic agents as potential anti-cancer therapies; however, the lack of target selectivity of such agents results in a narrow therapeutic window.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.672
      Issue No: Vol. 140, No. 7 (2020)
       
  • 658 Inactivating p53 is essential for nerve growth factor receptor to
           promote melanoma initiating cells-stemmed tumorigenesis
    • Authors: L. Jiang; S. Huang, J. Wang, H. Lu, S. Zeng, H. Lu
      Abstract: Nerve growth factor receptor (NGFR, CD271 or p75NTR) is highly expressed in melanoma initiating cells (MICs) and critical for their proliferation and tumorigenesis, and yet the underlying mechanism(s) remain largely elusive. We previously showed that NGFR inhibits p53 activity in a negative feedback fashion in other cancer cells. Here we report that this feedback inhibition of p53 by NGFR plays an essential role in maintaining the sphere formation (stem-like phenotype) and proliferation of MICs and in promoting MICs-derived melanoma growth in vivo.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.670
      Issue No: Vol. 140, No. 7 (2020)
       
  • 657 TGF-β2 upregulates OPN3 in human epidermal melanocytes
           independent of TGF-β2R in vitro
    • Authors: Y. Wang; H. Lu, Y. Lan, X. Yang, Y. Gu
      Abstract: Background: Previous studies have shown that OPN3 belongs to the photosensitive G Protein-Coupled Receptors families and is involved in the growth and melanogenesis of human epidermal melanocytes. However, the question whether OPN3 is regulated by TGF-β2 is yet to be answered. Objective: To explore the relationship between TGF-β2 and OPN3 in human epidermis melanocytes. Methods: Human epidermal melanocytes were stimulated with various concentrations of TGF-β2 in vitro. The expressions of OPN3 gene was monitored after the treatment of 10ng/ml TGF-β2, 10μM LY2109761 and 9 mM U73122.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.669
      Issue No: Vol. 140, No. 7 (2020)
       
  • 656 GDP/GTP exchange inhibitor, FR900359, synergizes with chloroquine in
           GNAQ/11-mutant melanoma
    • Authors: A. Truong; M. Onken, K. Blumer, M. McMahon
      Abstract: GNAQ and GNA11 (GNAQ/11) encode for G-alpha proteins, and mutations in these genes are found in approximately 2% of melanoma, including 80% of uveal melanoma. Constitutive activation of G-alpha proteins leads to downstream activation of multiple oncogenic pathways, such as MAPK signaling. Unfortunately, metastatic uveal melanoma is generally refractory to all available systemic therapies, including MAPK targeted therapies, indicating a critical need for novel therapies. Recently, the GDP/GTP nucleotide exchange inhibitor, FR900359, was characterized to inhibit growth of GNAQ-mutant uveal melanoma cells in vitro, but has been limited in translatability due to a narrow therapeutic window.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.668
      Issue No: Vol. 140, No. 7 (2020)
       
  • 655 α-MSH induces melanogenesis via up-regulation of Opsin1 in cultured
           human skin melanocytes in vitro
    • Authors: W. Zeng; J. Mou, H. Lu
      Abstract: Background: Physiological skin color change is regulated by environmental cues such as sun light and also modulated through the neurological and/or endocrine control of chromatophores. Opsin1 found in bird and fish pineal gland and hypothalamus was suggested to have roles in regulating skin color change. Our previous studies have shown that Opsin1 is expressed in human skin melanocytes. α-MSH is a well known endocrine hormone that induces pigmentation in human skin. However, the question whether this hormone interacts with Opsin1 in modulating human skin melanogenesis has not been fully addressed.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.667
      Issue No: Vol. 140, No. 7 (2020)
       
  • 653 The relationship between genetic mutations and clinicopathological
           manifestations in Korean cutaneous melanoma: A study based on
           next-generation sequencing
    • Authors: J. Byun; J. Heo, G. Choi
      Abstract: Identifying individual mutated genes have been proposed for effective biomarkers and therapeutic targets in cutaneous melanoma. Next-generation sequencing (NGS) shows comprehensive genomic mutations with high sensitivity. We assessed the genetic alteration in patients with melanoma and their prognosis according to clinicopathological manifestations. We analyzed clinicopathological features of 11 patients with melanoma who obtained surgical resection and examined genetic mutations using NGS. Among 11 cases of melanoma, gene mutations were identified in five.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.665
      Issue No: Vol. 140, No. 7 (2020)
       
  • 651 Risk factors for recurrence with topical imiquimod cream for lentigo
           maligna: Survival analysis with 17 years follow-up
    • Authors: M. Chambers; K. O'Hern, C. Baker, D. Badin, E. Saunders, M. Chapman
      Abstract: Topical imiquimod 5% cream has been investigated as an off-label primary or adjuvant treatment for melanoma in situ lentigo maligna (LM) type, although long-term follow-up data are lacking. In this single-institution retrospective analysis we evaluated treatment response and recurrence from 2002-2019 for LM treated with primary or adjuvant topical imiquimod (5%) with or without tazarotene gel 0.1% as pretreatment. 98 patients were identified with a total of 103 lesions analyzed. Mean follow-up time was 5.9 years (SD: 5.2), with 29.1% of cases having>10 years follow-up.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.663
      Issue No: Vol. 140, No. 7 (2020)
       
  • 650 Imiquimod spares disfiguring surgery for large facial lentigo maligna
    • Authors: K. O'Hern; M. Chambers, M. Chapman
      Abstract: Surgical excision of lentigo maligna (LM) in anatomically constrained sites such as the face can result in adverse functional and cosmetic consequences. Patient age, comorbidities, lesion size, or location can make adequate margins impossible and/or disfiguring. The off-label use of topical imiquimod has been considered as an alternative. This case represents clearance of LM in an 86-year-old female with a 10 cm facial LM involving the eyelid treated with topical imiquimod. An 86-year-old Caucasian female with no personal/family history of skin cancer presented with a 24-month history of an enlarging, 10 cm, irregularly brown, pigmented, macule, without nodules, ulcerations, or erosions on the left zygomatic cheek extending to the lateral superior and inferior eyelids.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.662
      Issue No: Vol. 140, No. 7 (2020)
       
  • 649 A retrospective multicenter study of melanoma in children and
           adolescents
    • Authors: S. Consortium
      Abstract: Background: Melanoma in the pediatric population is rare, with distinct clinical features compared to adult disease. Risk factors are not well understood in children and adolescents. This study aims to characterize melanoma in the pediatric population and explore potential risk factors and negative patient outcomes. Methods: Multicenter retrospective study of patients
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.661
      Issue No: Vol. 140, No. 7 (2020)
       
  • 648 Association between the frequency of indoor tanning and pain among
           women in the United States
    • Authors: B. Wang; J. Han, X. Li
      Abstract: Some people may use indoor tanning as a treatment for chronic pain. We tested the association of indoor tanning frequency with pain feeling and pain medication usage in the Nurses’ Health Study II, a large well-characterized cohort in the United States. The study population consists of 75,957 female Caucasian nurses. We used the linear regression model and logistic regression model for the association of indoor tanning frequency with pain feeling and pain medication usage, respectively. The frequency of indoor tanning is significantly association with pain after controlling for various confounding factors.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.660
      Issue No: Vol. 140, No. 7 (2020)
       
  • 646 Late-stage melanoma diagnosis in New York State (NYS)
    • Authors: P. Shah; S. Bajaj, D. Polsky
      Abstract: Early-stage (ES) melanoma at diagnosis is independently prognostic for improved survival compared to late-stage (LS) melanoma, defined as having regional or advanced spread. Additionally, decreased physician supply and lower socioeconomic status are associated with diagnosis at more advanced stages. We investigated population-level and county-level associations with LS diagnosis in NYS, a region of lower ambient sun exposure than areas frequently the subject of melanoma epidemiology studies in the US.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.658
      Issue No: Vol. 140, No. 7 (2020)
       
  • 645 5-(3’, 4’-Dihydroxyphenyl-valerolactone) regulates DNA methylation
           in UVB-irradiated keratinocytes
    • Authors: A. Kim; B. Mock, H. Lee, Y. Kang, D. Kim, J. Shin
      Abstract: 5-(3’,4’-Dihydroxyphenyl)-γ-valerolactone (DHPV) is a colonic microbial metabolite derived from cacao. In the previous study, we found that cacao powder modulates DNA methylation and inhibits UVB-induced AP-1 activation and MMP-1 expression. To further investigate whether major metabolite of cacao, DHPV, has UV protective effect as cacao, we evaluated the regulatory role of DHPV on DNA methylation in keratinocytes after UVB irradiation. In this study, we found that UVB irradiation altered methylation profiles in HaCaT cells and these changes were ameliorated by DHPV.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.656
      Issue No: Vol. 140, No. 7 (2020)
       
  • 643 XPC dissociation from damaged DNA and efficient global nucleotide
           excision repair depend on vitamin D receptor
    • Authors: C. Wong; D.H. Oh
      Abstract: Vitamin D and its receptor, VDR, together and independently, have been associated with DNA repair, though the mechanism by which they act is unclear. Upon ultraviolet irradiation through 3 mm pores in otherwise opaque filters to create focal spots of DNA damage, epidermal keratinocytes from both VDR-null mice and human keratinocytes depleted of VDR with siRNA exhibited slower removal of 6-4 photoproducts than normal control cells over 90 minutes. Co-staining with antibodies to XPC, the initial UV-induced DNA damage recognition sensor, revealed that XPC rapidly accumulated at DNA damage foci and gradually faded over 90 minutes as nucleotide excision repair proceeded in control human keratinocytes.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.654
      Issue No: Vol. 140, No. 7 (2020)
       
  • 641 The cutaneous response to solar UV radiation is attenuated by
           chlorination stress originating from swimming pool disinfectants
    • Authors: J. Jandova; A. Hua, S.E. Dickinson, A. Shakya, G.T. Wondrak
      Abstract: Hypochlorous acid (HOCl) is the active oxidizing principle released by standard swimming pool disinfectants used on a global scale, but the cutaneous consequences of human exposure to HOCl remain largely unknown, posing a major public health concern relevant to populations around the world. Here, for the first time, we have profiled the HOCl-induced cutaneous stress response in reconstructed human epidermis and SKH1 hairless mouse skin. In addition, we have investigated the molecular consequences of co-exposure to solar simulated ultraviolet (UV) radiation and HOCl, a procedure mimicking environmental exposure experienced by recreational swimmers.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.652
      Issue No: Vol. 140, No. 7 (2020)
       
  • 640 CrispR/Cas9 deletion of TLR4 impacts the UV-induced stress response in
           human keratinocytes
    • Authors: V. Kirschnerova; M. Khawam, B. Seligmann, C. Curiel, G.T. Wondrak, S.E. Dickinson
      Abstract: Damage incurred by exposure to ultraviolet (UV) radiation stimulates multiple stress-responsive signaling pathways in the skin. There is mounting evidence that Toll-like receptor 4 (TLR4) mediates key inflammatory responses in skin cells, including keratinocytes. Pharmacological blockade of TLR4 using resatorvid (TAK-242) inhibits not only acute UV-induced signaling in vitro and in vivo, but blocks UV-induced skin carcinogenesis in mouse models. In order to better characterize the UV-induced responses linked to TLR4, we have recently utilized CRISPR/Cas9 techniques to delete TLR4 from the genome of human HaCaT keratinocyte cells in culture.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.651
      Issue No: Vol. 140, No. 7 (2020)
       
  • 639 Association of clinical and demographic factors with phototherapy
           outcomes in patients with atopic dermatitis
    • Authors: D.D. Huh; Y.S. Roh, N. Kim
      Abstract: For many patients with Atopic Dermatitis (AD), first-line interventions fail to alleviate symptoms. UVA, PUVA, and UVB phototherapy have been utilized as a safe and cost-effective therapeutic options for a variety of inflammatory skin conditions including AD. We conducted a retrospective cohort study to examine the efficacy of phototherapy in the management of AD and to identify patient characteristics associated with treatment compliance and outcomes. We conducted a chart review of 145 AD patients treated at the Johns Hopkins Phototherapy Unit from 2009-2017 to characterize the demographics, comorbidities, concomitant medications, number of treatments, and UV irradiation dosing, as well as the post-treatment outcomes.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.650
      Issue No: Vol. 140, No. 7 (2020)
       
  • 638 Cyp11a1 derived secosteroid, 20(OH)d3 as a novel therapeutic agent for
           the prevention and treatment of uvb induced skin cancer
    • Authors: P. Guroji; S. Qayyum, T. Kim, Z. Janjetovic, M. Athar, A. Slominski
      Abstract: Non-melanoma skin cancers are more prevalent in people prone to repeated solar UVB expose. Though UVB is required for Vitamin-D3 production, yet it manifests tumorigenesis. 1,25(OH)2D3 protects from several autoimmune disorders and malignancies, however, due to its calcemic effects, therapeutic uses at pharmacological doses are limited. Previously, our lab reported that CYP11A1 produces 20(OH)D3 and is non-calcemic in mice and rats at pharmacological doses (60 μg/kg). Loss of PTCH gene function activates Sonic hedgehog (SHH) signaling, which drives BCC and perhaps SCC.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.649
      Issue No: Vol. 140, No. 7 (2020)
       
  • 637 Age and insulin-like growth factor-1 (IGF-1) status impact translesion
           synthesis (TLS) pathway activation in human keratinocytes and skin
           epidermis
    • Authors: M.G. Kemp; R.J. Hutcherson, A. Alkawar, A.J. Castellanos, R.D. Gabbard
      Abstract: Several studies have indicated that UV-induced DNA photoproducts are removed from epidermal genomic DNA at a slower rate in geriatric skin than in young adult skin. Such a situation may result in a greater need for the translesion synthesis (TLS) pathway of DNA replication, in which specialized DNA polymerases are recruited to sites of DNA damage to replicate across DNA adducts in either an error-free or error-prone manner. Here we show that skin epidermis from geriatric individuals (>65 years of age) exhibits higher levels of mono-ubiquitinated PCNA, a biochemical marker of TLS pathway activation, than the skin of young adults (aged 21-29) following exposure to 700 J/m2 of UVB light.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.648
      Issue No: Vol. 140, No. 7 (2020)
       
  • 636 Narrowband UVB (311nm) phototherapy maintains its high efficacy in
           psoriasis throughout repetitive treatment cycles
    • Authors: P. Wolf; M. Wiedenbauer, T. Graier, T. Benezeder, A. Gruber-Wackernagel, F. Legat, A. Hofer
      Abstract: In contrast to systemic agents, phototherapy is administered intermittently in the treatment of psoriasis. At least for systemic agents (such as biologics), there is a concern that after treatment interruption, restarting treatment may lead to a weaker therapeutic response compared to that of after initial treatment (e.g. due to formation of neutralizing antibodies). In this study, we analyzed the efficacy of narrowband UVB (311nm) phototherapy (NB-UVB) under daily life conditions in patients who had received one or more phototherapy cycles.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.647
      Issue No: Vol. 140, No. 7 (2020)
       
  • 635 Efficacy of a nomad SPF50+ sunscreen stick with broad spectrum
           protection in both UVB+A and NIR
    • Authors: M. Noizet; P. Lapalud, A. Tourette, A. Houcine, D. Bacqueville, T. Douki, C. Jean Decoster, S. Bessou-Touya, H. Coubetergues
      Abstract: Several studies have shown that the ultraviolet (UVB/A) from terrestrial solar radiation are strongly implicated in the etiology of most skin cancers via the generation of DNA lesions and reactive oxygen species (ROS). Moreover, skin is also exposed to solar near infra-red (NIR) radiation which are responsible of oxidative stress generation. Therefore, it is important to protect the most sensitized skins (actinic keratosis or atopic dermatitis) in all circumstances/all day long. Thus, the aim of this project was to develop a SPF50+ sunfilter product in stick form to provide a nomad protection for sensitized skins.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.646
      Issue No: Vol. 140, No. 7 (2020)
       
  • 634 Photoprotective efficacy of a new sunscreen formulation SPF50+ against
           chronic UV-induced skin damage in an ex vivo human skin model
    • Authors: D. Bacqueville; J. Severine, L. Duprat, M. Saint Aroman, S. Bessou-Touya, H. Duplan
      Abstract: Introduction: The prevalence of skin cancer has increased in last decade and is mostly due to chronic sunlight exposure. Therefore, it is important to prevent sun-induced skin damage by using photoprotection strategies. Objective: To assess the photoprotective efficacy of an innovative emulsion containing specific sunfilters combination to protect the skin against UV-induced lesions. Materials and Methods: An ex vivo human skin model was developed to mimic chronic exposure of solar-simulated radiation (CSSR).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.645
      Issue No: Vol. 140, No. 7 (2020)
       
  • 633 Low-level pulsed wave red light induces type I procollagen protein and
           ATP production at shorter treatment times as compared to continuous wave
           red light
    • Authors: W. Li; P. Thepmankorn, R. Parsa, M. Southall
      Abstract: Low-level light therapy uses non-thermal irradiance to modulate cellular functions via laser or light emitting diodes (LEDs). Red light has been clinically shown to stimulate wound healing, improve hair growth, relieve pain and inflammation as well as reduce skin wrinkles. Recent studies suggest that the biological responses to continuous wave light treatment may decrease or desensitize over time, and therefore continuous wave light treatment may not provide optimum efficacy. Thus, we sought to characterize the cellular effects of non-continuous "pulsed" wave (PW) vs continuous wave (CW) lights on induction of extracellular matrix gene expression in skin cells.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.644
      Issue No: Vol. 140, No. 7 (2020)
       
  • 632 UVB induced EMT-like phenotype in keratinocytes is mediated by TLR3
           activation
    • Authors: A. Schneider; R. Feehan, C. Garner, Z. Cong, A. Flamm, E. Billingsley, A. Nelson
      Abstract: Risk factors for non-melanoma skin cancer (NMSC) include UVB exposure, genetic mutations, immunosuppression, and chronic inflammation. Activation of Toll-Like-Receptor 3 (TLR3), which recognizes dsRNA, leads to downstream activation of NF-κB and an upregulation of inflammatory cytokines. TLR3 protein expression is higher in moderately differentiated squamous cell carcinomas (SCCs) and infiltrative basal cell carcinomas (BCCs) compared to well-differentiated SCCs by immunohistochemistry (n> 6) suggesting TLR3 expression correlates with more aggressive NMSCs.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.643
      Issue No: Vol. 140, No. 7 (2020)
       
  • 628 Evidence that wounding of geriatric skin which upregulates IGF-1
           levels protects against both abnormal carcinogenic UVB responses as well
           as from the development of non-melanoma skin cancer
    • Authors: J.B. Travers; M.G. Kemp, D. Spandau
      Abstract: UVB irradiation causes specific DNA damage to keratinocytes that can lead to cancer-causing mutations if they are allowed to persist in proliferating cells. Previously, we demonstrated that the activation status of the insulin-like growth factor-1 receptor (IGF-1R) regulates the cellular response of keratinocytes to UVB exposure. Briefly, geriatric skin is deficient in IGF-1 expression resulting in an aberrant IGF-1R-dependent UVB response consisting of basal keratinocytes proliferating while still harboring unrepaired DNA damage.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.639
      Issue No: Vol. 140, No. 7 (2020)
       
  • 627 UVB-generated microvesicle particles mediate systemic
           immunosuppression
    • Authors: L. Liu; C.M. Rapp, S. Zheng, J.B. Travers
      Abstract: Microvesicle particles (MVP) are small membrane bound particles released from cells that have been demonstrated to act as signal transporters between cells via their abilities to transport bioactive molecules. Previously our laboratory has reported that environmental injuries such as ultraviolet B radiation (UVB) and thermal burn injury can generate MVP release via a novel pathway involving the Platelet-activating factor (PAF) receptor in epithelial cell lines and human skin. Our current studies using pharmacologic and genetic approaches demonstrate that MVPs released from keratinocytes in vitro and mice in vivo in response to UVB (UVB-MVP) are dependent upon PAF receptor and acid sphingomyelinase (aSMase).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.638
      Issue No: Vol. 140, No. 7 (2020)
       
  • 626 Solar simulated light induces cutaneous SCC in inbred mouse strains:
           Development of a clinically relevant mouse model
    • Authors: A.C. Adams; A.M. Macy, H. Cui, J. Merida, S.E. Dickinson, D.J. Glembocki, D.J. Roe, K.T. Hastings
      Abstract: To address the need for clinically relevant cutaneous squamous cell carcinoma (cSCC) mouse models, we exposed SKH-1, FVB/N, Balb/c and C57BL/6 mice to solar simulated light (SSL), the environmental etiologic factor in human disease. Outbred SKH-1 mice are highly susceptible to SSL-induced tumor formation. However, an outbred strain limits the ability to evaluate MHC-restricted, antigen-specific T cell responses and perform studies with genetic mutants. The susceptibility of inbred strains FVB/N, Balb/c and C57BL/6 is unknown.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.637
      Issue No: Vol. 140, No. 7 (2020)
       
  • 624 Red light emitting diode (LED) light treatment promotes memory through
           up-regulation of trpm4 in Zebrafish
    • Authors: S. Yu; M. You, W. Yang, C. Cheng, H. Chang, H. Yu
      Abstract: Although light from light emitting diodes (LEDs) has become a very common and important environmental factor in our daily lives, there is limited research regarding LED light’s possible effects on biological functions on the skin and the brain. Herein, we investigated the long-term effects of white (WL), blue (BL), and red (RL) LED light on cognitive learning and memory recall by using a zebrafish animal model. Zebrafish that were 36 days post fertilization (dpf) were used for light exposure. Light intensity was 7.2 J/cm2 for 120 mins per day for 30 days.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.635
      Issue No: Vol. 140, No. 7 (2020)
       
  • 623 Macrophage depletion preserves dermal collagen in UVB exposed mice
    • Authors: M. Sharma; V. Werth
      Abstract: We previously reported that Etanercept blocks UVB-induced recruitment of neutrophils and macrophages into the dermis in mice, but paradoxically, accelerates loss of dermal collagen by increasing breakdown of mature collagen and decreasing synthesis of procollagen. To examine the role of macrophages in dermal collagen preservation during UVB exposure, we performed macrophage depletion in mice. Macrophages increase inflammation and tissue remodeling in many conditions by producing MMPs and TGFβ. MMPs can degrade collagen and TGFβ can induce procollagen synthesis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.634
      Issue No: Vol. 140, No. 7 (2020)
       
  • 621 A novel painless ALA-PDT in the treatment of skin diseases
    • Authors: X. Wang; Q. Zeng, L. Shi, P. Wang
      Abstract: Pain during conventional photodynamic therapy (C-PDT) is the main limiting adverse effect in its use in dermatology. During the course of C-PDT, most patients experience sensations of burning and tingling pain that vary in intensity. In some cases, patients have to stop the treatment because of unbearable pain. It is generally believed that C-PDT pain is mediated by free radicals generated by PpIX; ROS can either stimulate nerve endings directly or mediate pain through inflammatory by-products. Pain remains the top obstacle that prevents C-PDT application to all patients.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.632
      Issue No: Vol. 140, No. 7 (2020)
       
  • 620 UVB radiation with high photon density induces dendritic cell
           maturation and contributes to cutaneous immune suppression via Treg cell
           expansion
    • Authors: C. Lan; S. Huang, S. Lai
      Abstract: Phototherapy is an important treatment modality used in dermatology practice, and UVB radiation is often used for treating inflammatory skin disorders due to its immune suppressive effects. Currently, several UVB emitting radiation with different irradiance (W/cm2) are available. Clinical reports have shown different response profiles from devices emitting similar wavelengths at different irradiance. We previously showed that at equivalent fluence (mJ/cm2), high irradiance (HI) UVB imparted more immune suppressive effect as compared to its low irradiance (LI) counterpart.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.631
      Issue No: Vol. 140, No. 7 (2020)
       
  • 617 Skin squamous cell carcinoma (SCC)-derived exosomes after ALA-PDT
           treatment deliver microRNA-3473 to induce M1 macrophage polarization via
           NF-κB pathway
    • Authors: Q. Zeng; X. Wang
      Abstract: Topical ALA-mediated PDT, ALA-PDT, is a novel therapeutic modality widely used to treat actinic keratosis, Bowen’s Disease, superficial skin SCC, and other cancerous and precancerous skin diseases. Several studies have proved that ALA-PDT can inhibit SCC growth and reduce tumor volume. Subsequent research suggested that ALA-PDT not only directly induced tumor cells apoptosis, but also improve tumor microenvironment through regulation of immune cells. However, the anti-tumor immune function of ALA-PDT is still need to be elucidated.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.628
      Issue No: Vol. 140, No. 7 (2020)
       
  • 616 Synergistic effect between cigarette smoke and sunlight on human
           keratinocytes
    • Authors: A. Grenier; P.J. Rochette, R. Pouliot
      Abstract: Several environmental factors influence the skin aging process, including exposure to sun and cigarette smoke. Studied separately, these factors are known to accelerate skin aging. However, effects of a potential synergy between these two factors have been poorly characterized. It is well known that some cigarette smoke components can accumulate in the skin by contact or by systemic effect after inhalation and that solar rays can penetrate the epidermis and dermis. The aim of this study is to assess the harmful effects of this synergy on skin and more precisely on skin aging.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.627
      Issue No: Vol. 140, No. 7 (2020)
       
  • 615 Inverse docking assisted identification of flavonols as c-Kit, CDK2
           and mTOR inhibitors for melanoma and non-melanoma skin cancer management
    • Authors: T. Roy; S. Banang-Mbeumi, S. Boateng, R.N. Chamcheu, A. Walker, J. Chamcheu
      Abstract: Melanoma and non-melanoma skin cancers (NMSCs) are two major forms of skin cancers. Melanoma is the most aggressive form of skin cancer related deaths while basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) are most prevalent forms of NMSCs with over 5.3million yearly cases in the US. Due to huddles including resistance, bioavailability and adverse effects with available treatments, there is the need for effective strategies against skin cancer progression. The cyclin dependent kinase-2(CDK2), c-kit and mammalian target of rapamycin (mTOR), are attractive targets for new anticancer drug development.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.625
      Issue No: Vol. 140, No. 7 (2020)
       
  • 614 A novel treatment for skin repair using a combination of a MR
           antagonist + Vitamin D3
    • Authors: D. Biyashev; U.V. Onay, S. Evans, M. Demczuk, K. Lu
      Abstract: Exposure of the skin to toxic chemotherapy agents such as nitrogen mustard (NM) triggers activation of inflammatory dermal macrophages (Macs) with high expression of iNOS and TNFa resulting in delayed wounds. We have shown that Intervention with vitamin D3 (VD) mitigates Mac-mediated inflammation resulting in skin repair. Data from our in vitro drug screen using RAW 264.7-NFkB reporter cell line shows that VD with spironolactone (SP) has additive inhibition of NFkB activity. SP is a mineralocorticoid receptor antagonist known to be protective in chronic heart failure and other clinical conditions due to its abilitiesto delay progression of tissue injury and inhibit inflammation.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.624
      Issue No: Vol. 140, No. 7 (2020)
       
  • 610 AHR agonist RLV102 suppresses inflammation in mouse models of eczema
           and psoriasis
    • Authors: Y. Zhou; L. Tang
      Abstract: Aryl hydrocarbon receptor (AHR) signal transduction pathway is involved in barrier formation and regulation of inflammation of the skin and the digestive system. Recently tapinarof, an AHR agonist, has shown effect for treatment of psoriasis in mouse models and in early phase clinical trials, revealing therapeutic potential by modulating AHR activities. Here we describe development of RLV102, a potent next generation AHR agonist and report its effect on psoriasis and atopic dermatitis in animal models.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.620
      Issue No: Vol. 140, No. 7 (2020)
       
  • 609 The topical tanning agent dihydroxyacetone induces stress response
           gene expression and signaling in human reconstructed epidermis and SKH1
           hairless mouse skin
    • Authors: J. Perer; J. Jandova, J. Fimbres, E. Jennings, J. Galligan, A. Hua, G.T. Wondrak
      Abstract: Chemical tanning is widely regarded as a safe alternative to solar UV-induced skin tanning, but the cutaneous biology impacted by chemical tanning remains largely unexplored. Chemical tanning is based on the formation of melanin-mimetic cutaneous pigments (‘melanoidins’) from spontaneous glycation reactions between epidermal amino acid/protein components and reactive sugars including the glycolytic ketose dihydroxyacetone (DHA). Here, we have examined cutaneous effects of acute DHA exposure on cultured human keratinocytes, epidermal reconstructs, and mouse skin employing gene epression array analysis and immunodetection.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.619
      Issue No: Vol. 140, No. 7 (2020)
       
  • 608 Targeting CtBP-mediated proinflammatory gene transcription to treat
           skin inflammation
    • Authors: H. Li; C. Zhang, B. Li, M. Fujita, D. Norris, X. Wang, M. Huang
      Abstract: Inflammatory skin diseases (ISDs) such as psoriasis and allergic contact dermatitis affects millions of people and poses a major public health burden. Aberrant cytokine production is a prominent characteristic of ISDs, although the molecular mechanisms underlying the imbalance between pro- and anti-inflammatory gene expression remain underexplored. C-terminal-binding protein (CtBP) 1 and 2 are transcriptional coregulators that repress diverse cellular processes. Our recently studies have uncovered a previously unrecognized proinflammatory role of CtBP in skin inflammation.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.618
      Issue No: Vol. 140, No. 7 (2020)
       
  • 607 Targeting 14-3-3e-CDC25A interactions to trigger apoptotic cell death
           in skin cancer
    • Authors: T.R. Holmes; J. Al-Matouq, M. Holmes, N. Sioda, J. Rudd, L. Nicola, S. Lovas, L. Hansen
      Abstract: Non-melanoma skin cancer is the most common form of cancer worldwide. We previously documented an anti-apoptotic role for CDC25A in cutaneous squamous cell carcinoma (SCC), an activity dependent on its association with 14-3-3 proteins. We hypothesized that targeting CDC25A-14-3-3ε interactions may be an effective strategy for inducing skin cancer cell apoptosis. Co-immunoprecipitation revealed that CDC25A associated with 14-3-3ε, 14-3-3γ and 14-3-3ζ in SCC cells but not normal keratinocytes. Additionally, in SCC cells, overexpression of CDC25A induced Akt/BAD/Survivin pro-survival signaling while knockdown of 14-3-3ε inhibited pro-survival signaling, suggesting that 14-3-3ε and CDC25A similarly activate pro-surivval signaling pathways to suppress cell death.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.617
      Issue No: Vol. 140, No. 7 (2020)
       
  • 606 Small molecule blockade of T-type calcium channels by DX416 inhibits
           itch and reduces corresponding inflammation in acute and chronic itch
           mouse models
    • Authors: J. Kreitinger; L. Walker, V. Gadotti, N. Wageling, G. Zamponi, F. Diaz, P. Diaz
      Abstract: The processing of itch signals is regulated by many different ion channels. Among those are T-type calcium channels which are important regulators that contribute to the firing behavior of pruriceptors, or itch sensing neurons. The human genome encodes three different T-type channels: Cav3.1, Cav3.2, Cav3.3. It has been shown recently that pharmacological blockade of T-type calcium channels inhibits acute itch and that immune cells can express Cav 3 channels with roles in effector function. Thus, we hypothesized that local administration of the novel small molecule T-Type calcium channel inhibitor, DX416, would decrease cutaneous itch both through reducing pruriceptors signals triggering itch and decreasing local inflammatory immune responses potentiating itch.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.616
      Issue No: Vol. 140, No. 7 (2020)
       
  • 605 Long-term management of moderate-to-severe plaque psoriasis:
           Maintenance of treatment success following cessation of halobetasol
           propionate 0.01%/tazarotene 0.045% lotion
    • Authors: L. F. Stein Gold; M.G. Lebwohl, N. Bhatia, T. Lin, R. Pillai
      Abstract: Psoriasis is an immune-mediated disease that may have frequent remissions/exacerbations. Treating psoriasis by combining tazarotene (TAZ) with a topical corticosteroid, such as halobetasol propionate (HP), may enhance efficacy while reducing side effects of HP, which limit long-term use. TAZ also sustains response posttreatment and may play a role in maintenance therapy. This 1-year, open-label study assessed a fixed-combination, once-daily HP 0.01%/TAZ 0.045% lotion in participants with moderate-to-severe psoriasis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.615
      Issue No: Vol. 140, No. 7 (2020)
       
  • 603 Treatment of moderate-to-severe acne with once-daily tazarotene 0.045%
           lotion in pediatric patients: Pooled analysis of two phase 3 studies
    • Authors: L.F. Eichenfield; E. Tanghetti, E. Guenin
      Abstract: Acne, common in adolescence, can be treated with topical retinoids, such as tazarotene (TAZ). TAZ is an anti-inflammatory and comedolytic prodrug whose active form, tazarotenic acid, selectively binds retinoic acid receptors β and γ. A lower-dose 0.045% TAZ lotion was developed using polymeric emulsion technology, as gel or cream 0.1% TAZ formulations can cause irritation and limit use. In two phase 3, double-blind, vehicle-controlled 12-week studies (N=1,614), patients with moderate-to-severe acne were randomized (1:1) to receive TAZ 0.045% lotion or vehicle once-daily.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.613
      Issue No: Vol. 140, No. 7 (2020)
       
  • 602 Discovery of new olfactory receptors in human keratinocytes: A
           potential role on skin stress response
    • Authors: R. Duroux; A. Mandeau, Y. Quesnel, E. Loing
      Abstract: Olfactory receptors (ORs) are mainly expressed in the nasal olfactory epithelium but can also be found in different cell types throughout the body to regulate physiological cell functions beyond olfaction. Recently, ORs have been detected to be functionally expressed in the skin when activated with odorants. Olfaction is known to be disturbed under stress condition and is widely used in aromachology to afford relaxing effect. Therefore, we questioned the role of skin olfactory receptors in response to this environmental factor.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.612
      Issue No: Vol. 140, No. 7 (2020)
       
  • 600 Treatment of facial flushing and erythema by carvedilol in rosacea
           
    • Authors: J. LI; Z. Song
      Abstract: Introduction: Treatment of erythematotelangiectatic rosacea (ETR) is extremely challenging, because of the severe facial flushing and anxiety, which form a vicious cycle of reciprocal causation in the pathogenesis. There are no known specific treatments available, some cases have reported that beta-blokers play a role in the treatment of rosacea, but there are not yet any randomized controlled prospective, with larger seriers, clinical studies to evaluate the effects of systemic betabloker therapy in treating ETR.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.610
      Issue No: Vol. 140, No. 7 (2020)
       
  • 598 Identification of a human skin commensal bacterium that selectively
           kills cutibacterium acnes
    • Authors: A. ONeill; T. Nakatsuji, M. Williams, R. Mills, A. Hayashi, D. Gonzalez, R.L. Gallo
      Abstract: Cutibacterium acnes (C. acnes) is one of the most common bacterial species on human skin and can promote acne vulgaris. Most current therapeutics for acne are antibiotics that fail to discriminate C. acnes from the other skin resident microflora, many of which have important roles in health. Such approaches have significant drawbacks due to poor antimicrobial activity on the skin surface and emergence of antibiotic resistance. The microbiome represents a vast resource for drug discovery as its members engage in constant conflict to outcompete one another by deploying diverse strategies for survival.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.608
      Issue No: Vol. 140, No. 7 (2020)
       
  • 597 An Aquaphilus dolomiae extract modulates cutaneous sensitivity on in
           vitro models of neuro-inflammation
    • Authors: F. Lestienne; C. Viode, I. Ceruti, S. Carrere, S. Bessou-Touya, H. Duplan, N. Castex-Rizzi
      Abstract: Substance P is one of the neurotransmitters mainly involved in neurogenic inflammation on cutaneous level, a key point in the pathogenesis of inflammatory skin disorders such atopic dermatitis (AD), associated pruritus, and sensitive skin. In vitro models evaluated the effect of the original biological extract of Aquaphilus dolomiae E0 (Ad-E0) on cutaneous neurogenic inflammation. Ad-E0 significantly inhibited SP-stimulated release of IL-1β and TNF-α from normal human epidermal keratinocytes; significantly and dose-dependently inhibited SP-stimulated activation of human mast cells; significantly inhibited veratridine-stimulated release of SP from human sensory neurons; modulated expression of genes involved in lipid synthesis, innate immunity, corneocyte scaffolding and epidermal differentiation in a histamine-sensitized reconstructed human epidermis model; and, when applied topically to ex vivo human explants, inhibited IL-8 and histamine release.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.607
      Issue No: Vol. 140, No. 7 (2020)
       
  • 596 Complementary sun protection of two antioxidants (OTZ and DTG) in a
           sunscreen emulsion based on skin release
    • Authors: C. Jacques; D. Bacqueville, C. Jeanjean-Miquel, C. Genies, M. Noizet, A. Tourette, D. Redoules, S. Bessou-Touya, H. Duplan
      Abstract: Sunscreens have been shown to be extremely effective in preventing DNA lesions due to ultraviolet (UV) radiation. Indeed, recent studies indicate that the number of UV-induced lesions decreased up to 95% depending on sunfilter combination and sun protection factor (SPF). Thus, sunblockers protect the skin from the harmful effects of UV, but they do not provide 100% protection. As a result, sunscreens are now being developed with antioxidants to provide additional protection against oxidative stress-mediated cell damage especially reactive oxygen species (ROS).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.606
      Issue No: Vol. 140, No. 7 (2020)
       
  • 595 Differential ligand binding distinguishes therapeutic from pathologic
           Aryl Hydrocarbon Receptor (AhR) modulating agents: Implications for
           inflammatory skin disease
    • Authors: S. Smith; K. McHale, K. Creech, D. Rickard, C. Jayawickreme, D. Wu, F. Rastinejad, D. Rubenstein
      Abstract: The aryl hydrocarbon receptor (AhR) is a transcription factor highly expressed in epithelial cells and immune cells and is emerging as a therapeutic target for inflammatory skin disease. AhR activity can be influenced by several naturally occurring and manmade agonists and antagonists. Despite the discovery of 2,3,7,8-Tetrachlorodibenzodioxin (TCDD) as an AhR agonist, many compounds exert their effects via the AhR pathway, resulting in different physiological responses and clinical manifestations.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.605
      Issue No: Vol. 140, No. 7 (2020)
       
  • 594 Dynamic cytokine profiles combined with ELISPOT assay are useful in
           immunologically confirming the dapsone hypersensitivity syndrome
    • Authors: H. Liu; L. Sun, Q. Zhao, F. Zhang
      Abstract: Background: Dapsone hypersensitivity syndrome (DHS) is induced by drug-specific T cells. The specific dynamic cytokine profiles of DHS are unknown and the diagnosis of DHS in clinical practice remains a challenge. Objective: To investigate the DHS specific dynamic cytokine profiles, and explore specific enzyme-linked immunospot (ELISPOT) assay in the diagnosis of DHS. Methods: 14 types of cytokine levels (IFN-γ, Fas Ligand, Granzyme B, IL-4, IL-5, IL-6, IL-10, IL-13, IL-15, IL-17A, IL-21, IL-22, IL-23, and TNF-α) in DHS patients and dapsone tolerant patients carrying HLA-B*13:01 were analyzed using Luminex Bioplex assay.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.604
      Issue No: Vol. 140, No. 7 (2020)
       
  • 591 Pan-caspase inhibition is a novel immunotherapeutic against MRSA skin
           infections in mice
    • Authors: M. Alphonse; J.H. Rubens, R. Ortines, N. Orlando, A. Patel, D. Dikeman, Y. Wang, I. Vuong, D. Joyce, J. Zhang, M.M. Mumtaz, Q. Liu, C. Youn, G.J. Patrick, A. Ravipati, R.J. Miller, N. Archer, L.S. Miller
      Abstract: Staphylococcus aureus causes the majority of skin infections, and the emergence of methicillin-resistant S. aureus (MRSA) strains has created a public health threat. There is an unmet clinical need for non-antibiotic immunotherapies to combat MRSA. Herein, the pan-caspase inhibitor Quinoline-Val-Asp-Difluorophenoxymethyl ketone (Q-VD-OPH) was investigated for efficacy against an MRSA skin infection in mice. A single intraperitoneal injection of Q-VD-OPH 4 hour’s post-infection substantially decreased skin lesion sizes and rapidly reduced bacterial burden compared with vehicle or untreated wildtype (wt) mice.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.601
      Issue No: Vol. 140, No. 7 (2020)
       
  • 590 Increased psoriasis severity with obesity is associated with
           insufficient adiponectin-mediated regulatory T cell response
    • Authors: H. Liu; S.G. Yu, K.R. Kwan, M.S. Bonkowski, A.S. Paller
      Abstract: Psoriasis is a chronic inflammatory skin disease characterized by Th17 cell skewing. Obesity, the most common psoriasis co-morbidity, is associated with greater disease severity. We showed earlier onset and greater disease severity, as well as reduced adiponectin expression, in high fat diet (HFD)-fed obese mice compared to regular diet (RD)-fed controls when psoriasis-like disease is induced by topically-applied imiquimod (IMQ). Obesity is associated with a reduced Treg response, but its role in psoriasis is unknown.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.600
      Issue No: Vol. 140, No. 7 (2020)
       
  • 589 Effects of CCR4 antagonists in cutaneous T-cell lymphoma cells
    • Authors: J.S. Enriquez; P. Bijani, M. Duvic, X. Ni
      Abstract: CC chemokine receptor 4 (CCR4) is responsible for T-cell skin homing. It is overexpressed in type 2 helper T-cells (Th2) as well as cutaneous T-cell lymphoma (CTCL) cells. Mycosis fungoides (MF) and Sézary syndrome (SS) are two most common types of CTCL. Currently, Mogamulizumab, a humanized anti-CCR4 antibody, has been FDA-approved to treat MF and SS. However, the clinical response rates for SS patients was 30% and for MF patients was 22% and severe drug eruptions following treatment were reported.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.599
      Issue No: Vol. 140, No. 7 (2020)
       
  • 588 Identification of highly potent and selective Interleukin-1 receptor
           associated kinase 4 (IRAK4) degraders for the treatment of hidradenitis
           suppurativa
    • Authors: A. Slavin; V. Campbell, M. Mayo, H. Rong, X. Zheng, N. Ji, M. Weiss, S. Rusin, K. Sharma, J. Gollob, N. Mainolfi
      Abstract: Interleukin-1 receptor associated kinase 4 (IRAK4) plays a central role in myddosome signaling via kinase and scaffolding functions, making it an attractive target for the treatment of TLR- and IL-1R-driven inflammatory diseases. IL-1 family cytokines and TLRs, are central to the pathophysiology of hidradenitis suppurativa (HS), a Th1- and Th17-mediated neutrophilic, chronic inflammatory skin disease. Kymera has developed orally administered hetero-bifunctional molecules that selectively target IRAK4 for degradation and elimination by the ubiquitin proteasome pathway.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.598
      Issue No: Vol. 140, No. 7 (2020)
       
  • 587 Investigation of omega-3 mechanisms involve in psoriatic plaque
           healing using a psoriatic skin model produced by tissue engineering
    • Authors: M. Simard; S. Morin, G. Rioux, J. Fradette, R. Pouliot
      Abstract: Psoriasis is a dermatosis characterized by keratinocyte hyperproliferation, and abnormal epidermal differentiation. Clinical studies have shown that supplementation of the diet with omega-3 fatty acids could improve the symptoms of psoriatic patients. However, the mechanisms involved are still poorly understood.The aim of this study was to investigate the effects of α-linolenic acid (ALA) on the keratinocyte proliferation and differentiation of a psoriatic skin model. Healthy (HS) and psoriatic skin substitutes (PS) were produced according to the self-assembly method of tissue engineering, using culture media supplemented with 10 μM ALA.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.597
      Issue No: Vol. 140, No. 7 (2020)
       
  • 583 The evaluation of the application of glucocorticoids
    • Authors: X. Zhang; M. Pan
      Abstract: The administration of glucocorticoids is widely used in the treatment of bullous diseases. However, long-term use of systemic glucocorticoids will cause some side effects, such as infection, diabetes, hypertension, osteoporosis and so on. Recently, the topical steroids were applied to reduce the side effects. [1,2] In order to investigation the side effects between the topical and systemic application of the steroids, we tried to designed the experiments based on the betamethasone application on 8-week-old C57BL / 6 male mice.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.593
      Issue No: Vol. 140, No. 7 (2020)
       
  • 582 The discovery of new therapeutic combinations for Merkel cell
           carcinoma by small-molecule synergy screening
    • Authors: K. Garman; T. Gelb, D. Urban, M. Hall, I. Brownell
      Abstract: Merkel cell carcinoma (MCC) is a rare and highly metastatic neuroendocrine skin cancer. The majority of MCC tumors are virus positive (VP-MCC) and are associated with integrated Merkel cell polyomavirus, whereas the remainder of MCC are virus-negative (VN-MCC) tumors that are characterized by ultraviolet light associated mutations. Metastatic MCC is treated by immunotherapy or chemotherapy. Conventional chemotherapy is usually unbeneficial in MCC, and although immune checkpoint inhibitor (ICI) therapy can be effective, it is contraindicated in many patients and others have disease progression despite immunotherapy.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.592
      Issue No: Vol. 140, No. 7 (2020)
       
  • 580 Validation of an atopic dermatitis model in mice by repeated
           intra-dermal challenges with ovalbumin
    • Authors: H. Rashid; R. Samadfam
      Abstract: Background: Atopic dermatitis (AD) is a chronic inflammatory skin condition that significantly affects quality of life. A number of animal models have been developed over the years to mimic pathophysiology of AD. Among the different AD models, those induced by epicutaneous application of allergens and haptens are more common. However, limited absorption of peptide and protein allergens after epicutaneous application is challenging and requires long-term application of adhesive skin patches. In this present study, we examined induction of AD-like symptoms in mice with repeated intra-dermal challenges with the protein allergen, ovalbumin.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.590
      Issue No: Vol. 140, No. 7 (2020)
       
  • 579 Rationale and design for the Kallikrein Inhibitor in Netherton
           Syndrome (KINS) pivotal clinical trial
    • Authors: K.W. Johnson; A. Hovnanian, J. Teng, A.S. Paller, K. Choate, P. Elias, Z. Laura, T. Nguyen, D. Smith, A. Hsu, L. Alani, C. Lai, L. Hsu
      Abstract: Netherton Syndrome (NS) is a potentially life-threatening autosomal recessive disorder involving skin barrier breakdown, inflammation, and allergy. NS affects individuals from birth through adulthood, but standard interventions provide limited benefit and there are no FDA- or EMA-approved therapeutics. We summarize the Kallikrein Inhibitor in Netherton Syndrome (KINS) study, the first Phase 2/3, multicenter, randomized, double-blind, vehicle-controlled trial to evaluate the safety, efficacy and tolerability of a novel topical therapeutic (LM-030) in NS patients.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.589
      Issue No: Vol. 140, No. 7 (2020)
       
  • 578 Improved local drug delivery with bioadhesive nanoparticles in the
           treatment of skin cancer
    • Authors: J.K. Hu; H. Suh, M. Qureshi, J.M. Lewis, W.M. Saltzman, M. Girardi
      Abstract: Nanoparticles (NPs) have been utilized to enhance delivery of rapidly-degraded chemotherapies in multiple animal tumor models. We explored the role of NPs in the local treatment of squamous cell carcinoma (SCC). Using biodegradable poly(lactic acid)-hyperbranched polyglycerol (PLA-HPG), we encapsulated camptothecin (CPT) to create nonadhesive NPs (NNPs), which were then chemically converted to bioadhesive NPs (BNPs) that have previously exhibited augmented association with tumor microenvironments.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.588
      Issue No: Vol. 140, No. 7 (2020)
       
  • 577 Plasma exosomal miR-375-3p regulates ferroptosis in keratinocytes by
           targeting lipid transporter GPX4 in SJS/TEN
    • Authors: C. Zhang; G. Wang, M. Fu
      Abstract: Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are life-threatening, cutaneous adverse drug reactions that are accompanied by keratinocyte cell death. Ferroptosis is a recently recognized form of regulated cell death driven by lipid-based reactive oxygen species (ROS) accumulation. However, the molecular mechanisms of ferroptosis regulation are still largely unknown in SJS/TEN. Exosomes are nanometer-sized membranous vesicles in various body fluids. They contain functional proteins, mRNAs, and miRNAs.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.587
      Issue No: Vol. 140, No. 7 (2020)
       
  • 576 Crystal structure of sarecycline bound to the 70S bacterial ribosome
           reveals structural differences from other tetracyclines at atomic
           resolution
    • Authors: Z. Batool; I.B. Lomakin, Y. Polikanov, C.G. Bunick
      Abstract: Sarecycline is a new tetracycline-class antibiotic approved for the treatment of acne vulgaris. Tetracyclines share a common four-ring naphthacene core and inhibit protein synthesis by interacting with the 70S bacterial ribosome. Sarecycline is distinguished chemically from other tetracyclines because it has a 7-{[methoxy(methyl)amino]methyl]} group attached at the C-7 position of ring D. It represents the longest and largest C-7 moiety for any antibiotic in its class. To investigate the functional role of this C-7 moiety, we determined the crystal structure of sarecycline bound to the Thermus thermophilus 70S ribosome complex.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.586
      Issue No: Vol. 140, No. 7 (2020)
       
  • 575 GZ17-6.02 promotes autophagy and cell death in actinic keratoses via
           ATM-dependent mTOR inhibition
    • Authors: C. West; L. Booth, D. Von Hoff, S. Kwatra, P. Dent
      Abstract: GZ17-6.02 (602) is a novel investigational compound composed of curcumin, harmine, and isovanillin undergoing phase I clinical trials in oncology for solid tumors and lymphoma (NCT03775525). The goal of the present study is to determine the efficacy of 602 in killing actinic keratoses (AK) and to elucidate the molecular mechanisms underlying these effects. In a trypan assay, low concentrations of 602 killed AK cells to a greater degree than 5-fluorouracil (p
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.585
      Issue No: Vol. 140, No. 7 (2020)
       
  • 574 To assess the antifungal activity of Jublia® under real-world
           conditions
    • Authors: W. Lee; C. Otto, K. Abdul, D.M. Siegel
      Abstract: Onychomycosis affects up to 8% of the population and is most commonly caused by Trichophyton rubrum (T. rubrum) and Trichophyton mentagrophytes (T. mentagrophytes). Systemic antifungals provide higher rates of clearance compared to topical antifungals, but are associated with potentially serious side effects and drug-drug interactions. Efinaconazole, a triazole antifungal, is approved by the FDA as a 10% nail solution (Jublia®) for the treatment of distal and lateral subungual onychomycosis. Efinaconazole’s activity has been demonstrated using agar diffusion assays (ADAs) centered on cadaveric nail clippings; however, it has not been studied under real-world conditions that may provide a more accurate approximation of its true effectiveness.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.584
      Issue No: Vol. 140, No. 7 (2020)
       
  • 573 Improved effect on two cases of different subtypes of porokeratosis
           with superficial X-ray radiotherapy
    • Authors: Y. Chen; Q. Li
      Abstract: Porokeratosis (PK) is a heterogeneous group of skin disorders with unclear aetiology. This group includes multiple clinical variants. PK can cause cosmetic disturbances and poses the risk of malignant transformation. However, the current therapies for PK yield unsatisfactory results. To evaluate the therapeutic effects of superficial X-ray radiotherapy (SXRT) on PK, two patients with local PK who experienced failed multiple topical treatments were treated with SXRT. One patient with porokeratosis of Mibelli (PM) received one course of treatment for the lesion on their buttocks and two courses for neck lesions.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.583
      Issue No: Vol. 140, No. 7 (2020)
       
  • 571 Quantitative ligand and receptor binding studies reveal IL-36
           activation mode
    • Authors: V. Todorovic; L. Zhou, S. Kakavas, L. Wang, B. Sielaff, R. Sadhukhan, P.L. Richardson, E. DiGiammarino, C. Sun, V.E. Scott
      Abstract: IL-36 cytokines are pro-inflammatory members of the IL-1 family upregulated in a host of inflammatory skin disorders. They signal through the IL-36 receptor (IL-36R) and a shared subunit IL-1 receptor accessory protein (IL-1RAcP). Targeting antagonism of IL-36/IL-36R binding by biologics and small molecules is being actively pursued for several dermatological disorders. Therefore, it is critical to gain understanding of the mechanism of ligand-receptor binding. The mode of activation for the IL-36 pathway is proposed to be similar to IL-1 in that the IL-36 agonist forms a binary complex with IL-36R, which recruits IL-1RAcP.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.581
      Issue No: Vol. 140, No. 7 (2020)
       
  • 569 Rilzabrutinib (PRN1008) shows BTK-mediated mechanisms of action
           supporting clinical development for immune-mediated diseases
    • Authors: C. Langrish; M. Francesco, Y. Xing, J. Bradshaw, T. Owens, P. Nunn
      Abstract: Bruton tyrosine kinase (BTK) is a critical immune signaling enzyme expressed in B and innate immune cells and is an essential element downstream of BCR and FcR signaling. Rilzabrutinib (PRN1008) is an oral, reversible, covalent BTK inhibitor that drives durable BTK occupancy with low off-target effects shown by other BTK inhibitors. Preclinical PRN1008 activity was evaluated in biochemical studies and in vivo models of inflammation and canine pemphigus. PRN1008 showed kinase selectivity for BTK with an enzyme inhibition IC50 of 1.3 nM; functional BTK target occupancy of 91% (±2%) was achieved in PBMCs at 4 h.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.579
      Issue No: Vol. 140, No. 7 (2020)
       
  • 568 Comparing molecular cutaneous improvement in atopic dermatitis with
           various treatment modalities facilitates personalized approaches
    • Authors: J. Glickman; C. Dubin, J. Han, D. Dahabreh, S. Garcet, J. Krueger, A. Pavel, E. Guttman-Yassky
      Abstract: Atopic dermatitis (AD) is the most common chronic inflammatory skin disease. Recently, there is active therapeutic development in AD, with testing of targeted and broad treatments, to address various disease mechanisms. A comparison of the molecular effects of these treatments on AD skin abnormalities can facilitate a deeper understanding of the key pathogenic elements in AD, fostering further therapeutic developments, but it is still lacking. Using a meta-analysis-based approach, we are comparing immune and barrier effects of key systemic treatments used or tested in AD, including cyclosporine A, narrow-band ultraviolet B, dupilumab/anti IL-4R, fezakinumab/anti-IL-22, ASN002/JAK-SYK antagonist, ustekinumab/anti IL-12/23p40, as well as topicals (topical corticosteroids, crisaborole/anti PDE4) on lesions of AD patients.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.578
      Issue No: Vol. 140, No. 7 (2020)
       
  • 567 Mechanisms by which combined inhibition of BET and HDAC inhibits
           proliferation and induces apoptosis in CTCL
    • Authors: L. Zhao; G. Wood
      Abstract: Previously, we showed that the combination of BET inhibitors (BETi) and histone deacetylase inhibitors (HDACi) acted synergistically to induce significant apoptosis (60-80%) in CTCL lines and leukemic CTCL cells within 96 hours without significant apoptosis of normal CD4+ T cells (
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.577
      Issue No: Vol. 140, No. 7 (2020)
       
  • 564 Preclinical rationale for a first-in-human trial to evaluate the
           safety and preliminary efficacy of desmoglein 3 chimeric autoantibody
           receptor T cells (DSG3-CAART) for mucosal pemphigus vulgaris
    • Authors: A.S. Payne; J. Lee, D.K. Lundgren, X. Mao, S. Manfredo-Vieira, S. Nunez-Cruz, E.F. Williams, C. Assenmacher, E. Radaelli, B. Wang, C.T. Ellebrecht, J.A. Fraietta, M.C. Milone
      Abstract: We previously established proof-of-concept that antigen-specific B cell depletion can be achieved with gene-engineered T cells expressing chimeric autoantibody receptors (CAARs) comprising DSG3, the autoantigen in the autoimmune blistering disease mucosal pemphigus vulgaris (PV), fused to CD137-CD3ζ signaling domains. After specific lysis of anti-DSG3 B cells, DSG3-CAART cells are stimulated to proliferate and engraft, leading to potentially durable remissions of autoimmune disease. Here we present final preclinical studies supporting the DSG3-CAART investigational new drug application.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.574
      Issue No: Vol. 140, No. 7 (2020)
       
  • 563 ATx201 modulates biomarkers of skin barrier function and cutaneous
           inflammation in patients with moderate atopic dermatitis
    • Authors: E. Guttman-Yassky; R. Bissonnette, A. Pavel, E. Saint-Cyr Proulx, P. Prokocimer, R. Toft-Kehler, M. Sommer
      Abstract: ATx201 is a small molecule, which decolonizes Staphylococcus aureus and improves the diversity of the skin microbiome in patients with mild-to-severe atopic dermatitis (AD) (DECOLAD). We now report the safety and immune-modulatory effect of ATx201 in patients with moderate AD. In this randomized, double-blind, intraindividual, and vehicle-controlled Phase 2 trial, thirty-one patients received ATx201 CREAM 2% and matching vehicle (1:1) once daily for 3 weeks (NCT03304470), with a 12-day follow-up period.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.573
      Issue No: Vol. 140, No. 7 (2020)
       
  • 562 Biological evaluation of litchi derived products as dermatological
           agents
    • Authors: D. Huang; Z. Sun, X. Shen, Q. Gu, M. Soeberdt, C. Abels, J. Xu
      Abstract: Litchi (Litchi chinensis Sonn.) is a fruit native to South China, and popular for the taste and health benefits. Litchi products, such as litchi juice and vinegar (extracted or fermented from litchi fruit) have potential dermatological benefits. In this paper, we have evaluated litchi vinegar and juice regarding protective effects on HaCaT keratinocytes following either UVB irradiation or poly (I:C) by assessing cell survival (OD, optical density; MTT assay), reactive oxygen species (ROS), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.572
      Issue No: Vol. 140, No. 7 (2020)
       
  • 561 Development and first-in-human characterization of a potent oral CCR4
           antagonist for the treatment of atopic dermatitis
    • Authors: L.E. Cheng; A. Jorapur, S. Jacobson, O. Talay, S. Miakicheva, D. Trujillo, N. Lee, J. Jankicevic, D. Wustrow, P. Kassner, W. Ho, D. Brockstedt
      Abstract: Atopic dermatitis (AD) is predominantly driven by T helper type 2 cells (Th2). Accumulation of Th2 cells depends on CCR4-mediated recruitment of Th2 cells by the CCR4 ligands CCL17 and CCL22. Both are elevated in inflamed tissue, and levels correlate with disease activity and severity. Here, we describe RPT193, a novel, highly potent and specific oral CCR4 antagonist. In multiple preclinical mouse models of allergic skin inflammation, we demonstrated efficacy and reduction of Th2 cytokines with once daily dosing of RPT193 that is comparable to antibodies specific to IL-4 receptor and IL-13.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.571
      Issue No: Vol. 140, No. 7 (2020)
       
  • 560 Saikosaponin A induces HEKa cell apoptosis via ROS generation
    • Authors: M. Liu; Y. Zheng
      Abstract: Background: Psoriasis is a common, chronic and noninfectious skin disease with the complex pathogenesis which is reported as excessive proliferation and aberrant apoptosis of keratinocytes. Saikosaponin A (SSA) is a kind of triterpenoid saponin extracted from Chinese traditional medicine Radix bupleuri, possessing various biological functions such as anti-inflammatory, immune regulation and anti-tumor. But the effect of SSA on keratinocytes is unclear. Objective: To explore the effect of SSA on keratinocytes and to analyze the corresponding mechanism.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.570
      Issue No: Vol. 140, No. 7 (2020)
       
  • 559 Formulation and evaluation of topical products with Helianthus Annuus
           ozonized oil
    • Authors: M. Andreassi; C. Salvini, L. Furi, J. Zanardi, P. Brizzi, V. Travagli
      Abstract: This work was aimed to study the activity of topical products formulated with Helianthus annuus normal and ozonized oil produced in Italy, Recent studies have suggested a possible moisturizing and elasticizing activity of Helianthus Annuus ozonized oil. For such characteristics Helianthus Annuus ozonized oil appears an appropriate ingredient to be used in topical preparations for the treatment of skin hydration. The oil was incorporated into O/W emulsion in a standard formulation at percent concentration of 3%, 5% and 10 % respectively of Helianthus Annuus normal and ozonized oil, one O/W emulsion was prepared without active ingredient as control.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.569
      Issue No: Vol. 140, No. 7 (2020)
       
  • 557 IL-13 production and accumulation in lesional, non-lesional and ex
           vivo activated skin of atopic dermatitis patients
    • Authors: G. Isola; V. Gimenez-Riviera, C. Jack
      Abstract: IL-13 is a key cytokine mediating pathogenesis in atopic dermatitis patient skin. The cellular source and accumulation sites of this cytokine remain elusive in human skin, largely due to methodological limitations. Here we introduce three validated methodologies for the detection of IL-13 in skin biopsies and skin-derived T cells of patients with atopic dermatitis. Using confocal microscopy and flow cytometry, we show that IL-13 protein is highly sensitive to fixation with PFA, which masks key epitopes for the optimal detection by specific antibodies; this limitation requires the use of PFA-free protocols or heat-mediated protein retrieval for accurate quantification and study of this cytokine in the skin of patients.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.566
      Issue No: Vol. 140, No. 7 (2020)
       
  • 556 Safety and efficacy of leukocyte-rich platelet-rich plasma in the
           treatment of cicatricial alopecia
    • Authors: B. Khalid; M.K. Hordinsky, J.A. Shaik, R. Farah
      Abstract: Platelet-rich plasma (PRP) is defined as an autologous blood product with a concentration of platelets approximately three to five times the whole blood in a small volume of plasma. Due to the secretion of a plethora of growth factors by platelets in PRP that promote healing and tissue regeneration, its use has gained popularity to treat non-scarring alopecia, especially androgenetic alopecia, with promising results. However, there is limited data with only a few case studies reporting use and efficacy in scarring alopecia, wherein the hair follicle may be irreversibly destroyed due to inflammation and replaced by fibrous tissue.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.565
      Issue No: Vol. 140, No. 7 (2020)
       
  • 555 Practice changing landmark study- multi-institutional analysis of
           image guided superficial radiotherapy (IGSRT) for the treatment of
           non-melanoma skin cancer (NMSC)
    • Authors: L. Yu; D. Ladd
      Abstract: Practice changing landmark study- multi-institutional analysis of image guided superficial radiotherapy (IGSRT) for the treatment of non-melanoma skin cancer (NMSC). NMSC is generally treated in dermatology offices using surgical techniques. This study presents the largest multi-institutional retrospective modern series of Image Guided Superficial Radiotherapy (IGSRT) for Non-Melanoma Skin Cancer (NMSC). Between July 2013 and December 2019, 2424 NMSC lesions treated with IGSRT at 3 clinics in Texas and 1 clinic in NY were analysed.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.564
      Issue No: Vol. 140, No. 7 (2020)
       
  • 553 Clinical characteristics of acute graft-versus-host disease in small
           bowel and multi-visceral transplant recipients
    • Authors: C.M. Brumfiel; P.K. Dekker, K.M. Saardi, A.H. Kroemer, S.S. Kaufman, H.B. Pasieka
      Abstract: Acute graft-versus-host disease (aGVHD) following stem cell transplantation is characterized by rash, liver dysfunction, and diarrhea. Although aGVHD is uncommon following solid organ transplantation, we find higher rates of aGVHD after intestinal (ITx) and multi-visceral transplantation (MVT). In these cases we find a unique presentation as the donor liver (if present) and intestine are spared, leaving skin as the main target. Between 2013 and 2019, we diagnosed 17 cases of aGVHD following ITx (n=9) and MVT including both intestine and liver (n=8).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.562
      Issue No: Vol. 140, No. 7 (2020)
       
  • 552 Patient-centered development of a digital implementation tool for
           integrated knowledge translation with adult atopic dermatitis patients
    • Authors: C. Bouchard; S. Ghazal, N. Merati, G. Isola, M. Ehteshami, C. Jack
      Abstract: Targeted immuno-therapy for adult atopic dermatitis has left an important gap in understanding both by patients and physicians alike. International guidelines involve complex regimens of skin care, topical therapies, trigger avoidance, and behavioral adaptation first line. Engaging patients is key to communicating these fundamentals; however, resources are often limited at the point of care. Here we describe the development of an implementation tool for integrated KT in order to address this gap.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.561
      Issue No: Vol. 140, No. 7 (2020)
       
  • 551 Mid-level providers and the dermatology literature: A bibliometric
           analysis of trends 1973-2018
    • Authors: M. Maymone; M. Laughter, J.D. Jones, P. Anand, R. Dellavalle, J. Hugh, C. Dunnick
      Abstract: The number of mid-level providers (MLPs) in dermatology has grown tremendously over the last five decades. This increase may be due to the imbalance between the demand for dermatology services and dermatologists. Little research has been done to examine the evolving roles of mid-level providers and the state of scientific publications on this topic. To analyze the trends in publications and key topics related to MLPs in dermatology, PubMed was screened for all articles related to MLPs in dermatology published from 1973 to 2018.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.560
      Issue No: Vol. 140, No. 7 (2020)
       
  • 550 Mercaptopurine-induced Sweet syndrome in Crohn’s disease
    • Authors: J. Wat; M. Wat, K. Honda
      Abstract: Azathioprine-induced Sweet syndrome (AISS) usually occurs within a month of initiation of azathioprine. We describe a 19-year-old man with Crohn’s disease, which was well-controlled and chronically managed on 6-mercaptopurine (6-MP) and mesalamine, who presented with painful, hemorrhagic, centrally necrotic pustules on the bilateral dorsal hands and neutropenia. Pathology of a cutaneous lesion revealed a neutrophilic infiltrate, with a negative infectious source. Bone marrow evaluation showed hypocellular marrow consistent with 6-MP toxicity but no evidence of malignancy.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.559
      Issue No: Vol. 140, No. 7 (2020)
       
  • 549 Measurement properties of four different patient-reported outcomes to
           assess sleep disturbance in adults with atopic dermatitis
    • Authors: D.K. Lei; M. Yousaf, S. Janmohamed, P. Vakharia, R. Chopra, R. Chavda, S. Gabriel, R. Sacotte, K. Patel, V. Singam, S. Immaneni, R. Kantor, D. Hsu, D. Cella, J. Silverberg
      Abstract: Sleep is commonly impacted in atopic dermatitis (AD). However, the ideal patient-reported outcome measures to assess sleep in AD patients has not been determined. We sought to determine the measurement properties of the Patient-Reported Outcomes Measurement Information System (PROMIS) Itch Questionnaire (PIQ)-Mood and Sleep, Sleep Disturbance (SD), Sleep-Related Impairment (SRI), and Epworth Sleepiness Scale (ESS) in adults with AD. We performed a prospective dermatology practice-based study using questionnaires and evaluation by a dermatologist (n=491).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.558
      Issue No: Vol. 140, No. 7 (2020)
       
  • 548 Holistic dermatology: An evidence-based review of modifiable lifestyle
           factors associations with dermatologic disorders
    • Authors: S. Hu; P. Anand, M. Laughter, M. Maymone
      Abstract: Holistic dermatology focuses on treating the human body as a whole and implementing life-style changes to enhance the treatment and prognosis of skin disease. Understanding the interplay between modifiable life factors and patients’ dermatologic health will help physicians better inform patients on self-care methods to mitigate the burden of their skin disease(s). Our study reviews the current scientific literature on the relationship between modifiable life factors and dermatological outcomes of skin disorders.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.557
      Issue No: Vol. 140, No. 7 (2020)
       
  • 547 A variant subtype of psoriasis with a unique clinical and
           immunological characteristics
    • Authors: L. Zhang; L. Chen, F. Xue, J. Zheng
      Abstract: Objective We sought to characterize a variant subtype of psoriasis--eczematous psoriasis and its differences from psoriasis vulgaris. Method 5 patients diagnosed on out-patient and in-patient were selected as case group, meanwhile 11 psoriasis vulgaris and 11 eczema were selected as control group. We Assessed biopsy specimens using immunohistochemistry and quantitative real-time PCR, and cytometric bead array was used to detect inflammation-related cytokines in the serum. Result Except of basic pathological features of psoriasis, in lesion skin, eczematous psoriasis showed partial sponge edema and dermal vascular inflammation infiltration changes.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.556
      Issue No: Vol. 140, No. 7 (2020)
       
  • 544 Opioid use in all-cause chronic pruritus
    • Authors: T.T. Daugherty; E.M. Cole, R.A. Swerlick, S.C. Chen
      Abstract: Pruritus occurs frequently following administration of opioids, owing to its effect on μ opioid receptors and peripheral mast cell degranulation. However, the effect of opioids on pruritus in other primary skin conditions is unknown. We performed a retrospective chart review of patients with chronic pruritus, defined as itch lasting>6 weeks, to assess the association of opioid exposure with patient-reported itch severity, using a pruritus visual analogue scale (VAS). Opioid exposure was assessed using the Georgia Prescription Drug Monitoring Program (PDMP) records for opioid medication prescriptions filled within the 90 days prior to the appointment.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.553
      Issue No: Vol. 140, No. 7 (2020)
       
  • 543 Clinical and demographic features of morphea patients with
           mucocutaneous involvement: A cross sectional study from The Morphea of
           Adults and Children (MAC Cohort)
    • Authors: H.T. Jacobe; S. Prasad, S.M. Black, S. Sharma
      Abstract: While clinical findings of morphea have been described by our group and others, genital and oral lesions have not been well characterized. To address this knowledge gap, a cross-sectional analysis was performed of patients enrolled in The Morphea in Adults and Children Registry from 2007 to 2018. Of 737 patients analyzed, 48% (n=353) had linear morphea, 31% (n=232) had generalized morphea, 12% (n=87) had plaque morphea, and 6% (n=45) had mixed. Oral lesions were present in 23 patients (3%), of which 20 (87%) had linear morphea, nine had En Coup de Sabre (39%) and 12 had Parry Romberg Syndrome (PRS) (52%).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.552
      Issue No: Vol. 140, No. 7 (2020)
       
  • 542 The importance of IL-36 in palmoplantar pustulosis (PPP): An
           immunohistochemical analysis
    • Authors: R.E. Schopf; N. Assy
      Abstract: PPP is a rare and chronic disease that is characterized by sterile pustules, often with erythema, on the palms and soles. PPP may occur alone or in association with plaque-type psoriasis. The cause of PPP is not well understood; triggers include infections, smoking and genetic factors. At this time, treatment with cyclosporine is favorable, however, limited due to toxicity. To further elucidate pathogenesis we performed an immunohistochemical study of in 24 patients with PPP and in corresponding skin of 6 healthy control individuals.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.551
      Issue No: Vol. 140, No. 7 (2020)
       
  • 541 Anchoring the CLASI-A, a clinical outcome assessment (COA), to the
           patients’ perspective of their disease
    • Authors: N. Williams; K. Williams, V. Werth
      Abstract: The CLASI-A is a validated tool for physicians to quantify skin disease activity in cutaneous lupus and has been successfully used in several phase 1-3 clinical trials. Here, we evaluated the CLASI-A based on recent guidance from the FDA (USA) to focus on “meaningful within-patient change (i.e., improvement or deterioration from the patients’ perspective) in the concepts assessed by COAs.” We performed a prospective study on 250 patients with cutaneous lupus (1-25 clinic visits each). At nearly every visit, we recorded both a CLASI-A score by the physician (0-70; 70 is worst, 0 is best) and a visual analogue scale by the patient (ptVAS) to indicate his or her perception of cutaneous disease activity (0-10; 0 is worst, 10 is best), i.e., a patient global impression of severity (PGIS).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.550
      Issue No: Vol. 140, No. 7 (2020)
       
  • 540 Characterizing morphea subsets using a multi-center, prospective,
           cross-sectional analysis of morphea in adults and children
    • Authors: S. Prasad; J.L. Zhu, K. Schollaert-Fitch, R. Haley, K. Torok, H.T. Jacobe
      Abstract: Morphea is an inflammatory condition of the skin and soft tissue that results in excess collagen deposition, often producing functional and cosmetic impairment. To date, few large prospective studies have examined the clinical features of morphea. Furthermore, traditional subtype classifications have relied on expert opinion rather than unbiased analysis of clinical and demographic features of morphea patients. Using two combined prospective databases (n=944), we conducted a cross-sectional analysis using traditional univariate statistics and principal component analysis (PCA) to further characterize and identify clinically relevant subsets.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.549
      Issue No: Vol. 140, No. 7 (2020)
       
  • 539 Validation of the PROMIS Itch Questionnaire – itch severity
           assessments in adults with atopic dermatitis
    • Authors: J. Silverberg; J. Shei, K. Patel, V. Singam, P. Vakharia, R. Chopra, R. Sacotte, R. Kantor, D. Cella
      Abstract: Little is known about the validity of numeric and verbal rating scales (NRS and VRS) for itch and itch frequency for assessing itch severity in atopic dermatitis (AD). We evaluated the Patient-Reported Outcomes Information System (PROMIS®) Itch Questionnaire (PIQ) – itch severity assessment, including multiple NRS, VRS and frequency of itch assessments assessing a 7-day recall period, in adults with AD and compared their performance. Self-administered questionnaires and skin-examination were performed in 410 AD patients (age 18-90 years) in a dermatology practice setting.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.548
      Issue No: Vol. 140, No. 7 (2020)
       
  • 538 Itch-dominant atopic dermatitis: A distinct phenotype of atopic
           dermatitis
    • Authors: R. Chovatiya; J. Silverberg
      Abstract: Atopic dermatitis (AD) is a heterogenous disorder associated with multiple clinical phenotypes. In particular, AD patients may have different combinations of lesional and symptom severity, e.g. mild-moderate lesions with mild-moderate itch (mild-moderate lesions), mild-moderate lesions with severe itch (itch-dominant), or severe lesions and severe itch (severe lesions). Yet, little is known about how commonly these different AD subsets occur and their clinical characteristics. We sought to determine the characteristics and burden of itch-dominant AD.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.547
      Issue No: Vol. 140, No. 7 (2020)
       
  • 536 Comparing the Performance of Interferon Gamma Release Assays in
           Autoimmune Skin Diseases
    • Authors: D. Yan; R. Gaffney, R. Krain, E. Keyes, J.S. Concha, R. Feng, S. Schulz, L. Pappas-Taffer, R. Borucki, A. Ravishankar, J. Okawa, V. Werth
      Abstract: Autoimmune skin disease patients are screened for tuberculosis (TB) before initiation of immunosuppressive drugs via interferon gamma release assays (IGRAs). The two available tests, the QuantiFERON-TB Gold Plus (QFT-Plus, Qiagen, Hilden, Germany) and the T-SPOT.TB test (T-SPOT, Oxford Immunotec, Abingdon, UK) measure IFN-γ levels produced in response to T-cell stimulation with TB antigens. The T-SPOT test uses an enzyme-linked immunospot method rather than the enzyme-linked immunosorbent assay, the basis of the QFT-Plus test.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.545
      Issue No: Vol. 140, No. 7 (2020)
       
  • 534 Association of itch triggers with atopic dermatitis severity,
           persistence, flares and seasonality in adults
    • Authors: J. Silverberg; D. Lei, M. Yousaf, S. Janmohamed, P. Vakharia, R. Chopra, R. Chavda, S. Gabriel, K. Patel, V. Singam, R. Kantor, D. Hsu, D. Cella
      Abstract: Atopic dermatitis (AD) is associated with heterogeneous triggers of itch, which may impact AD course and severity. We sought to characterize the triggers of itch in adult AD. We performed a prospective dermatology practice-based study using questionnaires and evaluation by a dermatologist (n=334). Thirteen itch triggers were assessed using Patient-Reported Outcomes Measurement Information System® (PROMIS®) Itch-Triggers. Overall, 209 (62.6%) patients reported ≥1 itch trigger in the past week, and 114 (34.1%) having ≥3 itch triggers.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.543
      Issue No: Vol. 140, No. 7 (2020)
       
  • 533 Physician assessment of alopecia areata disease severity: Results from
           a real-world study
    • Authors: R. Burge; P. Anderson, C. Atkinson, D. Bell, J. Piercy, J. McCollam
      Abstract: Information on how severity of Alopecia areata (AA) is assessed is limited. The objective was to understand how physicians determine severity in AA patients in the real world. Data were drawn from the 2019 AA Disease Specific Programme, a cross-sectional survey of US dermatologists. Physicians completed an attitudinal survey covering perceptions of severity, then completed record forms for the next 5 patients consulting with AA capturing diagnosis, physician-subjective AA severity, % scalp hair loss, and other areas affected.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.542
      Issue No: Vol. 140, No. 7 (2020)
       
  • 532 Moderate to severe atopic dermatitis is associated with poor cognitive
           function
    • Authors: J. Silverberg; D. Lei, M. Yousaf
      Abstract: Atopic dermatitis (AD) is associated with itch, pain, sleep disturbance and systemic inflammation, all of which may contribute toward cognitive dysfunction. We sought to characterize the patient-burden of cognitive dysfunction in adult AD. We performed a prospective dermatology practice-based study using questionnaires and evaluation by a dermatologist (n=203). AD severity was assessed using patient-reported global AD severity, Patient-Oriented Eczema Measure (POEM), Numeric Rating Scale (NRS) for worst-itch and skin-pain, Dermatology Life Quality Index (DLQI), ItchyQOL, Eczema Area and Severity Index (EASI), objective and sleep components of Scoring AD (SCORAD).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.541
      Issue No: Vol. 140, No. 7 (2020)
       
  • 531 Impact of concomitant steroids on mogamulizumab efficacy in MAVORIC
    • Authors: O.E. Akilov; L. Geskin, M. Leoni, T. Ito, K. Dwyer, F. Herr, A. Musiek
      Abstract: Topical steroids are a mainstay of treatment for patients with mycosis fungoides (MF) and Sézary syndrome (SS). Therefore, patients undergoing treatment with mogamulizumab are likely to also receive steroid therapy and an understanding of the safety and efficacy of concomitant use is beneficial for physician decision-making. MAVORIC (NCT01728805) was an open-label, phase 3 trial in which patients with previously treated MF/SS were randomized 1:1 to mogamulizumab (1.0 mg/kg weekly for the first 28-day cycle, then Days 1 and 15 of subsequent cycles) or vorinostat (400 mg daily).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.540
      Issue No: Vol. 140, No. 7 (2020)
       
  • 530 A clinical comparison of photobiomodulation devices for the treatment
           of alopecia in all skin types
    • Authors: S. Pratapaneni; M.K. Hordinsky, R. Farah, L. Voller, M. Winter
      Abstract: Photobiomodulation (PBM) therapy is an emerging treatment for androgenetic alopecia (AGA). Although various devices are FDA-cleared for treatment (skin types I-IV only); no known trials to date have directly compared their outcomes against one another. Furthermore, these devices have not been studied in skin types of V-VI. We aim to clinically compare and evaluate the use of 4 FDA-cleared PBM devices in treating subjects of all skin types diagnosed with AGA. Subjects of skin types I-IV with AGA were randomized to PBM devices and received treatments per manufacturer’s recommendations.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.539
      Issue No: Vol. 140, No. 7 (2020)
       
  • 529 Profiling of phenotypes and plasma proteins identifies biomarkers for
           psoriasis severity and psoriatic arthritis
    • Authors: J. Walsh; M. Milliken, C. Carroll, S. Belman, K. Callis Duffin, G. Krueger, B. Feng
      Abstract: Biomarkers for psoriasis (Ps) and psoriatic arthritis (PsA) may elucidate the biological mechanisms of psoriatic diseases (PsD). We performed clinical phenotyping and plasma protein analysis on 234 Ps patients, including 44 with PsA, to identify protein profiles that associate with PsD. A physician-administered questionnaire was performed to determine Ps and PsA phenotypic features, including erythema, induration, scale, physician global assessment (PGA), body surface area (BSA), product of PGA and BSA (PGAxBSA), fingernail involvement, pustulosis, palmoplantar Ps, Koebner phenomenon, plaque size, plaque thickness, guttate Ps, inverse location, hyperlinearity of palms, erythrodermic disease, PsA, and duration of Ps and PsA at enrollment.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.538
      Issue No: Vol. 140, No. 7 (2020)
       
  • 527 Validation of the Skindex-mini in patients with atopic dermatitis
    • Authors: M.P. Dizon; C. Topham, D. Haynes, M. Brazil, M. Chren, E. Simpson
      Abstract: Quality of life (QOL) measurements may be used to better understand the patient experience of disease and judge the effectiveness of care, and numerous instruments have been developed within the field of dermatology. Validation of QOL instruments is critical in allowing clinicians and researchers to interpret and compare findings across studies. We sought to examine the measurement properties of the Skindex-mini (SDM), a brief three-item questionnaire created specifically for routine clinical practice.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.536
      Issue No: Vol. 140, No. 7 (2020)
       
  • 526 Computational modeling of two methods of histologic embedding during
           Mohs micrographic surgery
    • Authors: B. Carroll; S. Chu, E. Terrell, P. Govas, J. Oosten, L. Davidson, B. Ermentrout, D. Swigon
      Abstract: Skin cancer is the most common malignancy in the United States. Mohs micrographic surgery (MMS) combines surgical excision with oriented microscopic evaluation to provide the highest cure rate for non-melanoma skin cancers. Despite a theoretical 100% margin assessment, the recurrence rate for large skin cancers is as high as 41%. One potential reason for high recurrence rates is unanticipated tissue deformation during histologic processing. Two methods of embedding are the glass-slide and the heat sink embedding method.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.535
      Issue No: Vol. 140, No. 7 (2020)
       
  • 525 Worsening CLASI damage scores in patients with cutaneous lupus
           erythematosus may predict development of systemic lupus erythematosus
    • Authors: S.M. Black; F. Walocko, S. Anderson, X. Li, B. Adams-Huet, B.F. Chong
      Abstract: Up to 20% of cutaneous lupus erythematosus (CLE) patients develop systemic lupus erythematosus (SLE). Baseline characteristics associated with progression to SLE, including generalized DLE, have been previously identified. However, factors that change over time have not been well studied. The objective of our retrospective cohort study was to identify fixed and variable risk factors that predispose CLE patients to develop SLE. Our cohort consisted of 69 CLE patients without SLE at baseline followed for at least six months.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.534
      Issue No: Vol. 140, No. 7 (2020)
       
  • 524 Evaluating the utility of post-surgical radiation for high-risk
           cutaneous squamous cell carcinoma in immunosuppressed patients
    • Authors: L. Chang; B.A. Nguyen, R. Kazi, P. Govas, M. Pugliano-Mauro, B.T. Carroll
      Abstract: Cutaneous squamous cell carcinoma (cSCC) that presents with high-risk features such as perineural invasion is associated with a poor outcome. The National Comprehensive Cancer Network recommends consideration of post-surgical radiation for these aggressive tumors. However, the supporting evidence for this recommendation is limited for immunosuppressed patients. Recent studies demonstrated inferior outcomes of cSCC in immunosuppressed patients treated with adjuvant radiation compared with immunocompetent patients that also received radiation, highlighting the uncertain benefit of post-surgical radiation therapy in the immunosuppressed patient population.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.533
      Issue No: Vol. 140, No. 7 (2020)
       
  • 523 Evaluation of the impact and safety of DHA-containing camouflage on
           the repigmentation of vitiligo: An open-label self-controlled study
    • Authors: M. Li; F. Wang, X. Ding, J. Du
      Abstract: Background: Camouflage for vitiligo patients improves their quality of life. self-tanning products contained dihydroxyacetone(DHA) have been used for the camouflage of vitiligo lesions. So far, the use of DHA-containing camouflage is not being investigated. Objective:To evaluate the impact and safety of DHA-containing camouflage on the repigmentation of vitiligo. Methods:Thirty patients with vitiligo were enrolled in this study. 2 white patches were divided into 2 groups (group A, group B). Group A was treated with camouflage as needed , group B was a blank control.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.532
      Issue No: Vol. 140, No. 7 (2020)
       
  • 521 An intelligent assistant diagnosis study of erythema and scaly skin
           diseases based on deep learning
    • Authors: Y. Wang; D. Yuan, S. Zhao, X. Chen
      Abstract: This paper proposes an intelligent assistant diagnosis study of erythema and scaly skin diseases based on clinical images, including lichen planus, parapsoriatic, lupus erythematosus, eczema, and psoriasis. First of all, we collected a skin disease data set, which contains 4,377 clinical images. In this data set, each image contains the information of the lesion area and the patient's medical history. Then, four types of convolutional neural networks were used to classify erythema and scaly skin diseases, including InceptionV3, InceptionResNetV2, DenseNet121, and Xception.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.530
      Issue No: Vol. 140, No. 7 (2020)
       
  • 520 Establishing the analytical and clinical validity of sonographic
           biomarkers in hidradenitis suppurativa
    • Authors: D. Grand; J.W. Frew, K. Navrazhina, J. Krueger
      Abstract: Hidradenitis suppurativa (HS) is a chronic inflammatory dermatosis which currently lacks valid and reliable disease activity biomarkers. Non-invasive imaging modalities, such ultrasound, MRI, and medical infrared tomography, have demonstrated utility in detecting and characterizing subclinical HS lesions, however the analytical validity (correlating imaging with known histological and molecular markers of disease) and clinical validity (correlating imaging with validated clinical outcomes) have not been established.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.529
      Issue No: Vol. 140, No. 7 (2020)
       
  • 519 Evaluating world health organization essential medicines list for
           dermatologic disease
    • Authors: C.W. Rundle; A. Fortugno, O. Chosidow, L. Naldi, R. Hay, R. Dellavalle
      Abstract: The World Health Organization (WHO) Model List of Essential Medicines (MLEM) provides lists of medicines needed for basic healthcare systems. This study aims to appraise the dermatologic section of the WHO MLEM with respect to top Global Burden of Disease (GBD) skin disease entities. Skin burden data was extracted using the GBD results tool. Based on the top 13 GBD skin disease entities, dermatologic essential medicines were appraised with respect to Treatment of Skin Disease, 5th edition and Evidence-Based Dermatology, 3rd edition.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.528
      Issue No: Vol. 140, No. 7 (2020)
       
  • 518 Reported outcome measures in published Mohs micrographic surgery
           techniques: A systematic review
    • Authors: B. Gordon; P. Govas, B. Carroll, A. Hurst, C. Wessel
      Abstract: Mohs micrographic surgery (MMS) is a surgical excision technique that utilizes oriented microscopic evaluation to achieve higher cure rates for skin cancer than traditional surgical excision. In theory, the cure rate for MMS should be 100% due to its methodology, yet recurrences exist at a rate of 1%-41%. In clinical practice, recurrence rates of MMS may be impacted by considerable variability in the histologic techniques employed by MMS clinics. There is no gold standard for comparing MMS histology protocols.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.527
      Issue No: Vol. 140, No. 7 (2020)
       
  • 517 Leveraging CRISPR-Cas12a for the detection of human T-cell leukemia
           virus type 1
    • Authors: C. Baker; Y. Chen, M.S. Hayden
      Abstract: Human T-cell Leukemia Virus type 1 (HTLV-1) is a potent carcinogenic oncovirus that, while asymptomatic in the majority, has the potential to cause devastating complications such as adult T cell leukemia/lymphoma (ATLL) or HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Despite its global burden, poor access to current testing modalities limits HTLV-1 screening in endemic regions. Here we perform pre-clinical experiments that demonstrate the potential for a sensitive, specific, and low-cost CRISPR-based screening test for HTLV-1.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.526
      Issue No: Vol. 140, No. 7 (2020)
       
  • 515 Validation of nomogram incorporating clinopathologic factors and 31GEP
           test for predication of cutaneous melanoma patient recurrence risk
    • Authors: R. Thorpe; H.G. Caruso, K.R. Covington, D. Brodland, J.A. Zitelli
      Abstract: Patients diagnosed with cutaneous melanoma (CM) have important decisions to make early on with help of their physicians. We endeavored to develop a prognostication tool to facilitate this process. The 31gene expression profile (31GEP) is a prognostic test for CM that accurately predicts patients’ 5yr risk of recurrence, distant metastasis, and melanoma-related death. The 31GEP test stratifies patients as having lowest risk (Class 1A), intermediate risk (Class 1B/2A) or highest risk (Class 2B) and is an independent predictor of metastatic risk, as demonstrated in prospective and retrospective studies.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.524
      Issue No: Vol. 140, No. 7 (2020)
       
  • 514 Using design thinking to develop a decision aid for patients with
           psoriatic disease
    • Authors: M.T. Wan; M. Almonte, J. Gelfand, A. Ogdie
      Abstract: Starting or switching therapy in patients (pts) with psoriatic disease can be challenging given the available therapies and complexities of therapy selection and education. True shared decision-making is challenging during short visits, but pt decision aids (DAs) can assist this. This study aimed to understand pt and clinician needs in the process of starting/switching therapy and use design thinking to develop a pt-centered DA that addresses these needs. Design thinking, a method adopted from engineering, first seeks to understand user needs to develop a prototype which is then iteratively revised based on user input.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.523
      Issue No: Vol. 140, No. 7 (2020)
       
  • 513 Crisaborole in patients ≥3 months of age with mild-to-moderate
           atopic dermatitis (AD)
    • Authors: J.C. Su; L.J. Spelman, L.F. Eichenfield, L.F. Stein Gold, A. Cha, D. Graham, L. Takiya, J.L. Werth, C. Zang, B. Vlahos
      Abstract: Crisaborole ointment, 2%, is a nonsteroidal phosphodiesterase 4 inhibitor for the treatment of mild-to-moderate AD. We report the efficacy and safety of crisaborole across age groups in the phase 3 studies AD-301 (NCT02118766) and AD-302 (NCT02118792) and the phase 4 study CrisADe CARE 1 (NCT03356977). Patients aged 3 to
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.522
      Issue No: Vol. 140, No. 7 (2020)
       
  • 512 Patient and disease characteristic predictors of systemic exposure to
           crisaborole
    • Authors: V. Purohit; J.L. Werth, D. Graham, T. Nicholas
      Abstract: Crisaborole ointment, 2%, is a nonsteroidal phosphodiesterase 4 inhibitor for the treatment of mild-to-moderate atopic dermatitis (AD). Initial pharmacokinetic (PK) studies of crisaborole showed absorption with measurable systemic levels of crisaborole. A nonlinear regression analysis using ointment dose and noncompartmental PK parameters at steady state (area under the curve [AUCss] and maximum concentration [Cmaxss]) was conducted to correlate systemic exposure parameters with ointment dose and clearance difference between patient subpopulations.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.521
      Issue No: Vol. 140, No. 7 (2020)
       
  • 511 TWEAK is a critical cytokine linking vulgaris, pustular, and
           erythrodermic psoriasis
    • Authors: S. Wang; Y. Liu, H. Wang
      Abstract: Psoriasis is a common chronic inflammatory skin disease where various cytokines play a detrimental role. Tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) has been implicated in the pathogenesis of a variety of inflammatory disorders and autoimmune diseases. However, studies conducted on the relationship between TWEAK and various psoriasis patients are limited. In this study, we aimed to explore the serum and lesional levels of TWEAK in psoriasis patients and investigated whether TWEAK is associated with clinical variables and expression of other well-known psoriasis-related cytokines including IL-17a, IFN-γ, IL-22, IL-36γ.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.520
      Issue No: Vol. 140, No. 7 (2020)
       
  • 510 Successful treatment of vitiligo with cold atmospheric
           plasma-activated hydrogel
    • Authors: S. Zhai; Y. Xia
      Abstract: Nowadays, vitiligo shows insufficient response to current therapies, partially due to excessive oxidative stress and T lymphocyte dysfunction in lesions. We have known that cold atmospheric plasma (CAP) is capable of modulating immune responses. In this study, we sought to explore the therapeutic effect of CAP on patients with active focal vitiligo. A retrospective case series of 15 patients received topical application of CAP-activated hydrogel (some lesions treated with vehicle control), followed by scoring of lesional areas, and gp100-positive cells or CD8+ T lymphocytes were measured in biopsies.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.519
      Issue No: Vol. 140, No. 7 (2020)
       
  • 509 PIQ-C, a new PROMIS® tool, measures intensity and impact of itch on
           children with atopic dermatitis
    • Authors: A.S. Paller; J. Shei, S. Rangel, D. Ramachandran, N. Puar, V. Patel, K. Jackson, J. Silverberg, D. Cella
      Abstract: Itch in atopic dermatitis (AD) compromises quality of life. Most itch assessments focus only on itch intensity. Our goal was to develop and validate a comprehensive PROMIS (Patient Reported Outcomes Measurement Information System®) Itch Questionnaire - Child (PIQ-C). Concept elicitation to generate the PIQ-C item pool was based on: a) literature review; b) 15 semi-structured interviews with children aged 8-17 and parents of children aged 5-17 with itch; and c) theme assessment by independent reviewers.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.518
      Issue No: Vol. 140, No. 7 (2020)
       
  • 507 Alternative data presentation methods: Exploring waterfall and bubble
           plots in psoriasis biologic clinical trials
    • Authors: P. Surmanowicz; S. Emam, R. Kelm, A. Du, R. Gniadecki
      Abstract: Background: Modern patient care is built upon evidence-based medicine and relies on clinical trials to provide the empiric data to be interpreted and transformed into guidelines utilized by clinicians. Current psoriasis biologic clinical trials utilize a frequentist model (i.e. reporting statistical differences in the proportion of responders) and the PASI (psoriasis activity and severity index) score as the primary outcome measure. Although convenient and simple, these traditional methods lack reporting on individual patient data and cohort response distributions, and are therefore limiting in the conclusions they present to readers.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.516
      Issue No: Vol. 140, No. 7 (2020)
       
  • 506 Predicting the long-term outcomes of biologics in psoriasis patients
           using machine learning
    • Authors: S. Emam; A. Du, P. Surmanowicz, S. Thomsen, R. Griener, R. Gniadecki
      Abstract: Background: Real-world data demonstrate that approximately 50% of psoriasis patients treated with a biologic agent will discontinue the drug because of a loss in efficacy. History of previous therapy with another biologic, female sex, and obesity were identified as predictors of drug discontinuations, but their individual predictive value is low. Objectives: To determine whether machine learning algorithms can produce models that can accurately predict outcomes of biologic therapy in psoriasis on an individual patient level.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.515
      Issue No: Vol. 140, No. 7 (2020)
       
  • 505 Immunostimulatory herbal supplements in patients with autoimmune skin
           diseases
    • Authors: A. Ravishankar; D. Yan, C. Bax, J.S. Concha, B.E. Shields, L. Pappas-Taffer, R. Feng, J. Okawa, V. Werth
      Abstract: Complementary and alternative medicine (CAM) use is prevalent in dermatology. Certain CAMs, including Spirulina, Alfalfa, Chlorella, and Echinacea have been reported to be immunostimulatory or induce dermatomyositis (DM), cutaneous lupus erythematosus (CLE) or autoimmune blistering diseases (AIBD). As such, there is a need to characterize CAM usage in patients. We performed a retrospective chart review at UPenn to characterize CAM use among patients with DM, CLE, AIBD, and non-autoimmune controls.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.514
      Issue No: Vol. 140, No. 7 (2020)
       
  • 504 Deep learning empowered computer-assisted diagnosis on smartphone skin
           images
    • Authors: J. Wu; J. Han, Y. Zhang, J. Hu, Q. Li
      Abstract: Nowadays deep learning has been widely applied in identifying skin disease using clinical skin images. Along with the development of smartphone and communication technology, telemedicine becomes more accessible for patients to consult with medical experts. However, rare research was done to prove the feasibility of using deep learning to predict skin conditions based on smartphone captured skin images, especially taken by non-professional patients. We collected 46946 skin images from the online patient-doctor telemedicine platform in mainland China.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.513
      Issue No: Vol. 140, No. 7 (2020)
       
  • 503 Outcomes of patients with cutaneous T-cell lymphoma (CTCL) treated
           with extracorporeal photopheresis (ECP): A single institution experience
    • Authors: A.G. Johnson; Q. Zhao, C. Chung, B.M. William
      Abstract: ECP is an established therapy for CTCL yet patterns of utilization, complication rates, and outcomes vary widely among treatment centers. We hereby report our experience with 13 patients (pts) treated with ECP from 2013-2019; median age was 65 (range 47-87) years. All pts had advanced stage CTCL; 8 (61.5%) with mycosis fungoides (MF) and 5 (38.5%) with Sezary syndrome (SS). Median time from diagnosis to pheresis start was 2.8 months with a median of 2 lines of prior therapy. All pts initially started on a ECP regimen of 2 consecutive days per week with 8 (61.5%) pts concurrently using oral bexarotene (Bex) and subcutaneous interferon alpha (IFN), 2 pts on ECP alone (15.4%), 2 (15.4%) with Bex only, and 1 (7.7%) with IFN only.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.512
      Issue No: Vol. 140, No. 7 (2020)
       
  • 502 Atopic dermatitis patient focused drug development and the
           MoreThanSkinDeep Survey
    • Authors: W. Smith Begolka; K. Capozza
      Abstract: Atopic dermatitis (AD) is an inflammatory skin disease with significant physical, psychological, and social impacts, yet limited effective treatments. As part of the September 2019 MoreThanSkinDeep Patient Focused Drug Development (PFDD) meeting on AD, an online survey collected insights on disease and current treatment burdens, and unmet therapeutic needs from 1,508 AD patients and caregivers. Most respondents reported current moderate (44%) or severe (30%) AD, yet 80% noted severe AD at its worst, and 60% had overall worsening or unchanged AD since initial onset.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.511
      Issue No: Vol. 140, No. 7 (2020)
       
  • 501 Trial in progress: VALO study evaluating PTX-022 in adults with
           moderate-to-severe pachyonychia congenita, a rare, chronically
           debilitating disease that makes walking difficult or impossible
    • Authors: J. Teng; B. Shroot, H. Evans, J. Schwartz, J. Connor, J. Valentine, C. Hansen
      Abstract: INTRODUCTION: In pachyonychia congenita (PC), mutations in keratin genes cause skin fragility and impaired skin barrier function, resulting in severe plantar pain, ultimately making walking difficult or impossible. Currently, there are no approved therapies to treat PC. The keratins involved in PC are regulated by the mammalian target of rapamycin (mTOR) pathway. mTOR inhibitors therefore may repress the mutant keratin genes at the root of PC. In studies, oral mTOR inhibitors were shown to provide clinical benefit to patients.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.510
      Issue No: Vol. 140, No. 7 (2020)
       
  • 500 Ixekizumab achieves more rapid reduction of circulating interleukin-19
           compared to guselkumab in a psoriasis head-to-head study
    • Authors: R.J. Konrad; B.J. Nickoloff, R.E. Higgs, L.K. Ferris, K. Reich, H. Elmaraghy, G. Gallo, L. Renda, S. Park, J.G. Krueger
      Abstract: Previous results of a randomized, head-to-head trial between the interleukin (IL)-17A inhibitor ixekizumab (IXE) and the IL-23 inhibitor guselkumab (GUS) showed that significantly more patients treated with IXE than GUS had fully clear skin (PASI 100) after 4, 8, and 12 weeks (wks) of treatment.1 The circulating level of the pro-inflammatory cytokine IL-19, produced by activated keratinocytes and macrophages, is a prognostic marker for skin disease activity which is elevated ∼7-fold in psoriasis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.509
      Issue No: Vol. 140, No. 7 (2020)
       
  • 499 Long-term efficacy of secukinumab in Korean patients with
           moderate-to-severe plaque psoriasis in a real-world setting; A
           single-center, retrospective study
    • Authors: J. Park; S. Lee
      Abstract: This study was to evaluate the long-term efficacy of secukinumab in real-world practice in Korean patients with moderate-to-severe plaque psoriasis. A total of 19 patients with moderate-to-severe plaque psoriasis, who had been treated with 300mg secukinumab were analyzed by classifying them into naïve and bio-switched groups. As a primary end-point, therapeutic efficacy of secukinumab to reach PASI 75, PASI 90, and PASI 100 response was analyzed at week 52. For long-term efficacy and drug survival of secukinumab, 11 patients were analyzed up to week 104, including three patients who were followed-up until week 280-345.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.508
      Issue No: Vol. 140, No. 7 (2020)
       
  • 498 Patient safety alert: Medical image manipulation as a safety hazard
           for wrong-site procedures
    • Authors: J.S. Taylor; P.L. Bailin, A.T. Vidimos, J. Lucas, A. Vij, A.C. Halpern, D.K. Dennison
      Abstract: While some medical images are manipulated for patient benefit- radiograph rotation to aid during surgery and visible image enhancement to demonstrate plastic surgical outcomes, others have been manipulated causing patient harm- inadvertent flipping of x-rays resulting in wrong- side brain surgery, and an absence of photographs has caused wrong-site surgery in dermatology. We surveyed our department staff as well as the landscape of basic medical imaging devices, software, practices and current standards to ascertain actual and potential errors.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.507
      Issue No: Vol. 140, No. 7 (2020)
       
  • 497 Efficacy of immunotherapy in Merkel cell carcinoma patients with
           chronic immunosuppression
    • Authors: L. Zawacki; K. Lachance, T. Akaike, P. Nghiem
      Abstract: Merkel cell carcinoma (MCC) has a high propensity for recurrence and distant metastasis. Persons with chronic immunosuppression have a higher risk of developing MCC and a more aggressive disease course. Immune-checkpoint inhibitors (e.g. anti-PD-(L)1) are associated with improved disease-specific survival. However, the effectiveness and side-effect profile of these agents in immunosuppressed MCC patients is not well categorized in part because they were ineligible for prior clinical trials. This study assesses the risk-benefit profile of immunotherapy in this setting, and explores differences between forms of immunosuppression.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.506
      Issue No: Vol. 140, No. 7 (2020)
       
  • 496 Evaluation of extracts containing red clover using dermal papilla
           cells in the development of a scalp treatment system targeting hair loss
           and hair damage
    • Authors: B. Cook; J. Namkoong, M. Riggs, K.C. Holley, D. Kern, H. Knaggs
      Abstract: Hair loss and hair disorders are common conditions affecting millions around the globe. The causes are complex and not fully understood but links to genetics, lifestyle, intrinsic aging, and environment impact are all suggested. Prevalence is known to increase with age and response to treatment is notoriously variable. Treatments have a global market value of $2.5 billion (USD) driven by increasingly long and healthy lifespans, emphasis on aesthetic appearance, and the considerable emotional distress experienced by patients.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.505
      Issue No: Vol. 140, No. 7 (2020)
       
  • 495 The impact of psoriasis and its associated comorbidities on quality of
           life: Results from the National Psoriasis Foundation Annual Survey
    • Authors: S. Bell; J. Domire, J.F. Merola, A. Gottlieb, G. Gondo
      Abstract: In 2019, the American Academy of Dermatology and the National Psoriasis Foundation issued joint care guidelines for the treatment of psoriasis that highlighted the need to address comorbidities related to psoriatic disease. For nearly 20 years, the NPF has conducted a national survey of the psoriatic disease community. The survey seeks to enhance the understanding of the impact of psoriatic disease and its associated comorbidities on the health outcomes of individuals living with psoriasis and psoriatic arthritis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.504
      Issue No: Vol. 140, No. 7 (2020)
       
  • 494 Association of granuloma annulare with type II diabetes, cigarette
           smoking, and liver disease: A case-control study
    • Authors: E. Almazan; M. Belzberg, C.X. Qin, K. Williams, J. Choi, B. Kaffenberger, Y. Semenov, J. Alhariri, S. Kwatra
      Abstract: Granuloma annulare (GA) is a cutaneous granulomatous disorder of unknown etiology. There are conflicting reports of associations between GA and a range of systemic diseases. We conducted a retrospective case-control study of GA patients to clarify the relationship of GA with multiple systemic conditions. The electronic medical records of patients age 18 or older who presented to the Johns Hopkins Hospital System between January 1, 2009 and June 1, 2019 were reviewed under IRB approval. Inclusion criteria required a clinical and histopathological diagnosis of GA by a dermatologist.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.503
      Issue No: Vol. 140, No. 7 (2020)
       
  • 493 A deep neural network for the early image diagnosis of Stevens-Johnson
           syndrome/toxic epidermal necrolysis
    • Authors: A. Fujimoto; Y. Iwai, S. Muramatsu, T. Ishikawa, S. Shinkuma, R. Abe
      Abstract: Background: Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN) are life-threatening cutaneous adverse drug reactions (cADRs). However, distinguishing SJS/TEN from non-severe cADRS is often difficult, especially in the early stages of disease. To overcome this limitation, we developed a computer-aided diagnosis system for the early diagnosis of SJS/TEN powered by a deep convolutional neural network (DCNN). Methods: We trained a DCNN using a dataset of 25,054 individual lesion images obtained from 113 patients diagnosed with SJS/TEN or non-severe cADRs.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.502
      Issue No: Vol. 140, No. 7 (2020)
       
  • 492 The usefulness of an inexpensive, battery-powered, handheld microscope
           in low-resource dermatologic practices
    • Authors: N.D. Flint; S. Simister, I. Flint, T. Dickerson, A.M. Secrest
      Abstract: Previous studies in low- and middle-income countries have shown that skin disease is very common, especially dermatophyte and scabies infections. Point-of-care microscopic evaluation of skin scraping samples improves the diagnostic accuracy of these skin pathologies. Resource limitations, however, often preclude the use of microscopy for point-of-care evaluations in low- and middle-income countries. An inexpensive (
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.501
      Issue No: Vol. 140, No. 7 (2020)
       
  • 491 Interim results from a comparative method validation study evaluating
           the use of digital photographs versus in-person assessment of rosacea
    • Authors: R. Hamidi; H. Aralis, K.W. Bruhn, I.J. Deluca, K. Nguyen, M. Tran, M. Patel, V.A. Patel, J. Cotliar, B.H. Tan, H.L. Sofen, C.V. David
      Abstract: Photography-based teledermatology has the potential to increase patient access to clinical trials, in part by removing geographic barriers that limit participation to those who reside near a trial site. Here we present interim results from a study evaluating digital photography as a method of assessing rosacea severity, in comparison with in-person methods. Eight dermatology raters performed in-person assessments of rosacea patients at a clinic in Los Angeles, California. Rosacea severity was graded using the Clinician’s Erythema Assessment (CEA), Investigator’s Global Assessment (IGA), and inflammatory lesion count.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.500
      Issue No: Vol. 140, No. 7 (2020)
       
  • 490 Feasibility of examining fluorescence of follicular sebum and C. acnes
           after pool water immersion
    • Authors: R.A. Kimball; M. Porter, A.B. Kimball
      Abstract: Sebum is an oily waterproof semi liquid composed of triglycerides, fatty acids, wax esters, squalene, and cholesterol. Swimming pool water has been proposed to reduce water content of swimmers’ skin due to a reduction and possible rebound of sebum. Pool water is generally treated with hypochlorite and other chemicals which would not be anticipated to emulsify sebum, as cleansers do, but as oxidizers, could lead to some dissolution. Additionally, water immersion alone has been reported to increase trans epidermal water loss and the PH of volar skin.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.499
      Issue No: Vol. 140, No. 7 (2020)
       
  • 489 Words matter: A randomized controlled study evaluating the impact of
           decision framing on treatment preferences in adults with psoriasis and
           psoriatic arthritis
    • Authors: A. Kassardjian; V.S. Chat, L. Archuleta, J. Hekmatjah, T. Sierro, C. Read, A.Y. Chen, I. Singh, A.W. Armstrong
      Abstract: It is unknown how clinicians’ wording of a treatment influences patients preferences. Decision framing is the way that a choice is worded. A choice can be worded either positively (gain-framed) to explain the benefits of a therapy, or negatively (loss-framed) to explain the risks of not taking a therapy. We conducted a randomized controlled study to evaluate the effect of gain versus loss framing on patients’ treatment preference. Ninety adults with psoriasis ± psoriatic arthritis were randomized 1:1 to receive a questionnaire that contains either (1) a gain-framed message that explains the benefits of receiving an injectable medication for psoriasis and psoriatic arthritis, or (2) a loss-framed message that explains the harms associated with not taking the medication.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.498
      Issue No: Vol. 140, No. 7 (2020)
       
  • 488 Intravenous ertapenem therapy for advanced hidradenitis suppurativa
    • Authors: K. Babbush; M. Ghias, A. Nosrati, K. Pacific, H. Hosgood, S. Cohen
      Abstract: Ertapenem, a broad-spectrum antibiotic, has been used as an off-label treatment option for refractory hidradenitis suppurativa (HS). Previous studies have reported a significant improvement in quality of life, pain, and amount of drainage for HS patients treated with intravenous (IV) ertapenem. Additionally, key inflammatory markers, including erythrocyte sedimentation rate [ESR], c-reactive protein [CRP], interleukin-6 [IL-6], and tumor necrosis factor-alpha [TNF-a]), may be associated with disease severity in HS patients.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.497
      Issue No: Vol. 140, No. 7 (2020)
       
  • 487 Smoking is associated with the severity of rhododendrol-induced
           leukoderma and with the occurrence of leukomelanoderma
    • Authors: Y. Fukunaga; K. Fukai, A. Umekoji-Hayashi, T. Ishihara, A. Shintani, D. Tsuruta
      Abstract: Rhododendrol (RD) is a skin whitening ingredient that was developed in Japan. Among the 800,000 users of RD-containing cosmetics, 20,000 patients developed localized leukoderma (RD-induced leukoderma). Forty-two % of those users showed perilesional hyperpigmentation (leukomelanoderma), and 14% of them were associated with vitiligo vulgaris afterwards. The aim of this study is to investigate the risk factors affecting the severity of RD-induced leukoderma, the occurrence of leukomelanoderma, and the association with vitiligo vulgaris.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.496
      Issue No: Vol. 140, No. 7 (2020)
       
  • 486 The global burden of acne vulgaris: Results from the GBD study 2017
    • Authors: A. Grada; C.K. Aksut, R. Dellavalle, M. Naghavi
      Abstract: Background: Acne vulgaris is a common inflammatory disease that can be associated with significant psychological comorbidities. Updated estimates of acne disease burden are necessary in order to evaluate the impact of past healthcare policies and orient new healthcare strategies to address unmet needs. Methods: Data was extracted from scientific literature, national surveys, claims data, and primary care sources on the prevalence of acne. Prevalence data were combined with a disability weight to yield years lived with disability.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.494
      Issue No: Vol. 140, No. 7 (2020)
       
  • 485 The global burden of pressure ulcers: Findings of the GBD 2017 study
    • Authors: A. Grada; C.K. Aksut, R. Dellavalle, M. Naghavi
      Abstract: Introduction: Pressure (“decubitus”) ulcers present a challenge to health care professionals worldwide. The Global Burden of Disease (GBD) 2017 study is a systematic analysis to quantify the comparative magnitude of health loss at the global level. This study presents GBD 2017 results for pressure ulcers. Design and Setting: Pressure ulcer epidemiologic data sources were derived from an extensive literature search, hospital insurance data, and vital registration records. Data sources were analyzed with a Bayesian meta-regression modeling tool, DisMod-MR 2.1, to yield prevalence estimates.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.493
      Issue No: Vol. 140, No. 7 (2020)
       
  • 484 FDA cleared devices produce inconsistent platelet-rich plasma product
    • Authors: R. Farah; J.A. Shaik, M. Inyang, N. Sadick, N. Hussain, R. Freese, G. Gregorich, J. Asaila, M.K. Hordinsky
      Abstract: Platelet-rich plasma (PRP) has emerged as a novel option to treat androgenetic alopecia. However, data focused on quality of product from FDA cleared devices is lacking. Herein, we assessed and compared cellular PRP content produced from FDA cleared devices. Within one academic institution, white blood cell (WBC) counts and platelet counts from whole blood and resultant PRP were retrospectively reviewed. Institutional quality control (QC) procedures were utilized to obtain counts. Of the fifty-two PRP samples assessed, only 2 met the Insitutions QC standard (defined a 3-fold increase in platelet concentration).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.492
      Issue No: Vol. 140, No. 7 (2020)
       
  • 483 Hidradenitis suppurativa in a cohort of sixty years and older
    • Authors: K. Pacific; K. Babbush, S. Cohen
      Abstract: Hidradenitis suppurativa (HS) is a chronic, recurrent skin disorder characterized by painful nodules, pustules, purulent abscesses, and sinus tracts (“tunnels”) leading to progressive disability. While the epidemiology of HS has been reported, this has not been explored in patients 60 years and older. Our study examines this cohort. We identified 51 patients aged 60 and older with HS who were seen Montefiore Medical Center between 2015 and 2019. There were 39 females (76.5%); 26 (50.9%) self-identified as black or African-American, 7 (13.7%) as white, 11 (21.6%) as other, and 7 (13.7%) declined to respond or were unsure.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.491
      Issue No: Vol. 140, No. 7 (2020)
       
  • 482 Longitudinal cohort study of the association between atopic dermatitis
           and depression throughout childhood
    • Authors: C. Kern; J. Wan, K. Lewinn, S. Langan, K. Abuabara
      Abstract: Atopic dermatitis (AD) is one of the most common inflammatory diseases of childhood and has been associated with comorbid mental health outcomes, but there are limited data on the development of depression during childhood and adolescence, which may be a critical period for intervention. To understand origins of the relationship between AD and depression, accounting for heterogeneity in AD disease activity and severity over time, we analyzed data from an existing longitudinal cohort study representative of the UK population including 13,979 children followed from birth into adolescence.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.490
      Issue No: Vol. 140, No. 7 (2020)
       
  • 481 Reliability of self-reported data on social media vs. National
           Residency Match Program Charting Outcomes for dermatology applicants
    • Authors: S. Hu; M. Laughter, R. Dellavalle
      Abstract: Social media has risen to the forefront of online resources that dermatology residency applicants use to solicit advice and gauge the competitiveness of their application. However, there is a need to examine National Residency Match Program (NRMP) charting outcomes and self-reported academic metrics on SDN and Reddit, the two most popular social media forums used by health professional students. In this study, we compared combined match data from both SDN and Reddit to NRMP Charting Outcomes (2014, 2016, 2018).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.489
      Issue No: Vol. 140, No. 7 (2020)
       
  • 480 Health-related quality of life and economic burden of chronic pruritus
    • Authors: K. Whang; Y. Semenov, R. Khanna, K. Williams, V. Mahadevan, A. Huang, S. Kwatra
      Abstract: Chronic pruritus is a common dermatologic condition with a significant effect on quality of life (QoL). However, its impact on health-related QoL and economic burden has yet to be fully characterized. Our study aimed to measure the health-related QoL and societal economic implications of chronic pruritus. A cross-sectional survey of 95 patients with chronic pruritus was administered using the Ontario Health Utilities Index Mark 3 (HUI3) questionnaire. Normative population data (n=4,187) from healthy US adults were obtained from the 2002-2003 Joint Canada/United States Survey of Health.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.488
      Issue No: Vol. 140, No. 7 (2020)
       
  • 479 A retrospective review of 23 cases of pyoderma gangrenosum: Effects of
           comorbid conditions on treatment response
    • Authors: H. Nymeyer; H.K. Wong
      Abstract: Pyoderma gangrenosum (PG) is frequently associated with comorbid conditions such as inflammatory bowel disease, rheumatoid arthritis, and malignancy, but there is a paucity of data on how these comorbid conditions impact the response of PG to treatment. We retrospectively review 23 cases of PG treated at one medical institution to understand the effect that comorbid conditions have on the time to resolution of the PG. Of the 23 cases examined, 10 (43%) are associated with an inflammatory bowel disease or autoimmune hepatobiliary disease (PG with IBD).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.487
      Issue No: Vol. 140, No. 7 (2020)
       
  • 477 Pilot study examining blood cell count ratios as a predictor of
           subsequent non-melanoma skin cancers
    • Authors: S. O'Neal; A. Ravishankar, B. Lindgren, N. Goldfarb
      Abstract: Blood cell count ratios, including neutrophil-lymphocyte ratio (NLR), eosinophil-to-lymphocyte ratio (ELR), and monocyte-to-lymphocyte ratio (MLR) have prognostic implications for various malignancies. Typically, elevated ratios portend a worse prognosis, but a decreased NLR has been found in patients with NMSC. We examined the association between blood count ratios with risk of subsequent NMSC, and total number of NMSC, within 3-years of initial NMSC diagnosis. We performed a nested, retrospective study of patients in the Minneapolis VA Health Care System who had their first NMSC in 2003, and a CBC with differential within 30 days prior to or 60 days after the date of NMSC diagnosis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.485
      Issue No: Vol. 140, No. 7 (2020)
       
  • 476 Utilization and impact of immunotherapy in stage IV melanoma using the
           National Cancer Database
    • Authors: R. Conic; R. Knackstedt, G. Damiani, B. Gastman
      Abstract: The majority of data regarding immunotherapy for melanoma stems from trials that exclude important populations. Thus, while trials may demonstrate favorable results, not all populations may be represented. To evaluate factors affecting the utilization of immunotherapy and to stratify results based on the approval of ipilimumab in 2011 and PD-1 inhibitors in 2014, an analysis of available data from the National Cancer Database (NCDB) was performed. Stage IV melanoma patients were identified. Effects of immunotherapy on overall survival (OS) were assessed using Kaplan Meier curves and Cox proportional hazards model.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.484
      Issue No: Vol. 140, No. 7 (2020)
       
  • 475 A retrospective study of myocardial abnormalities detected on cardiac
           magnetic resonance imaging among patients with psoriasis compared to
           inflammatory skin disease controls
    • Authors: M. Goldenberg; M. Reynolds, S. Smart, J. Kaffenberger, S. Raman, B. Kaffenberger
      Abstract: Psoriasis is an inflammatory dermatological disorder that has been associated with various cardiac manifestations. The goal of this study was to evaluate the rates of cardiac inflammation and edema on cardiac MRI in psoriasis patients compared to atopic dermatitis and rosacea control patients so that cardiac magnetic resonance imaging (CMR) can be used as a potential biomarker for psoriasis patients in the future. This was a retrospective chart review that looked at CMR records for markers of cardiac inflammation in adult patients with psoriasis, atopic dermatitis, or rosacea who were patients at The Ohio State University Wexner Medical Center between 2011 and 2018.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.483
      Issue No: Vol. 140, No. 7 (2020)
       
  • 474 Single-center retrospective review of the use of checkpoint inhibitors
           in merkel cell carcinoma patients
    • Authors: M. Babadzhanov; N. Doudican, C. Ovits, T. Canavan, M. Stevenson, A. Pavlick, J. Carucci
      Abstract: Background: Given the role of the immune system in merkel cell carcinoma (MCC), immune-based therapies, including avelumab, pembrolizumab, and nivolumab, are currently FDA-approved as first-line treatment options for metastatic MCC patients. We sought to retrospectively review the efficacy of the use of checkpoint inhibitors (CPI) in MCC patients at our institution. Methods: We performed a single center retrospective review of MCC patients seen at NYU Hematology and Oncology departments between 2012-2018.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.482
      Issue No: Vol. 140, No. 7 (2020)
       
  • 473 Quality of life impact from skin diseases among persons living with
           HIV in Atlanta, Georgia
    • Authors: T. Rogers; B.C. Ly, E. Anderson, H. Yeung
      Abstract: HIV remains a public health crisis in the Southern United States and disproportionately affects sexual, gender, and racial/ethnic minority persons. With advances in antiretroviral therapy, the burden of HIV-related skin diseases is often assumed to fall accordingly. The quality of life impact of these skin diseases has not been recently quantified. Medical records of 165 new patients seen at the HIV Dermatology Clinic at Grady Memorial Hospital Ponce De Leon Center in Atlanta, Georgia were reviewed from January to August 2019 in a cross-sectional study.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.481
      Issue No: Vol. 140, No. 7 (2020)
       
  • 472 Visit complexity reflects billed level of service and documentation
           burden
    • Authors: J. Ryan Wolf; Y. Xie, I. Kim, A.P. Pentland, B.T. Pentland
      Abstract: The electronic medical record (EMR) promised healthcare efficiency, but has increased documentation burden for providers without yielding insight into patient outcomes. It is unclear how the EMR documentation workflow relates to the billed level of service (LOS) for clinic visits. We asked if the complexity of EMR documentation during a clinic visit correlates with the visit LOS. ThreadNet is a novel, R coded, graph-theoretic methodology that converts threads of sequence data into event networks to calculate visit complexity based on actions, roles, and workstations.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.480
      Issue No: Vol. 140, No. 7 (2020)
       
  • 471 Risk of psoriatic arthritis in psoriasis patients on biologics and
           methotrexate
    • Authors: E.N. Lininger; S.A. Siegel, K.L. Winthrop, A. Deodhar, S. Kiwalkar, A. Ortega-Loayza
      Abstract: Psoriatic arthritis (PsA) is a chronic inflammatory arthritis characterized by joint and entheseal inflammation seen in 30% patients with psoriasis (PsO). Inhibitors of tumor necrosis factor and interleukin 23 and 17 (‘biologics’) are efficacious treatment options for both. We aimed to determine if the use of either systemic non-biologic or biologic therapy decreases the risk of incident PsA in patients with PsO. Records on all PsO patients seen at dermatology clinic from January 2006 - June 2019 were reviewed.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.479
      Issue No: Vol. 140, No. 7 (2020)
       
  • 470 Development of a psoriasis severity score for clinical measures in a
           claims database
    • Authors: S.A. Siegel; J. McGregor, A. Blauvelt, J.R. Curtis, K.L. Winthrop
      Abstract: Greater psoriasis disease severity is associated with higher prevalence of co-morbidities and differences in prescribing patterns. To understand effects caused by different therapies, researchers need to accurately account for disease severity. To address this gap in knowledge, using a gold standard for psoriasis severity, we developed and validated a score to predict severity in a large administrative database. Two registries, the Center for Excellence in Psoriasis and Psoriatic Arthritis (CEPPA) at Oregon Health & Science University (OHSU) and the Corrona national psoriasis registry, were linked with Medicare data for 2006-2017.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.478
      Issue No: Vol. 140, No. 7 (2020)
       
  • 469 Evaluating melanoma incidence and survival in the US veterans
           population
    • Authors: J. Remer; A. Fonseca, M.A. Weinstock
      Abstract: Introduction: Melanoma incidence and mortality in the US military and veteran population is complicated. While limited, prior results suggest that melanoma incidence is increased in air force members and mortality is increased in former prisoners of war. As the 5th most common cancer in the veteran population who receive care at the VA, melanoma incidence and survival is still poorly understood. Purpose: To compare incidence and survival for malignant melanoma in the US veterans who received care at the VA to the general population.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.477
      Issue No: Vol. 140, No. 7 (2020)
       
  • 468 Clinical characteristics, etiology, and treatment of erythema
           multiforme at a tertiary care center
    • Authors: G. Shi; B. Kaffenberger, Y. Semenov, J. Choi, K. Williams, J. Alhariri, S. Kwatra
      Abstract: Erythema multiforme (EM) is an acute, immune-mediated condition characterized by distinctive skin lesions and occasional mucocutaneous involvement. EM is understudied, with limited epidemiologic studies. This study sought to describe etiologies, clinical characteristics, and treatment of EM patients seen at Johns Hopkins Hospital between 2009-2019. Data was retrospectively collected for all patients with ICD-9CM/ICD-10 codes for a primary diagnosis of EM. A total of 155 patients were included, of whom 84 (54%) were female and 71 (46%) were male with a mean age at time of onset of 34.5 years.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.476
      Issue No: Vol. 140, No. 7 (2020)
       
  • 467 Neurotic excoriations: A retrospective cohort study of disease
           presentation, comorbidities, and treatment in 250 patients at a tertiary
           care center
    • Authors: C. Kwon; R. Khanna, K. Williams, Y. Semenov, N. Kim, S. Kwatra
      Abstract: There is a lack of epidemiologic data on patients with neurotic excoriations (NE). We thus conducted a retrospective chart review of patients with NE from 2007 to 2019 at a tertiary care center. Patients with ICD-9/10-CM code L98.1 from January 1, 2007 to May 1, 2019 were identified (n=315). Patients (n=250) with the diagnosis of “neurotic excoriations,” “skin-picking,” “excoriation disorder,” or equivalent synonym were identified. Patient demographics, systemic and psychiatric comorbidities, clinical course, psychiatric medications, duration of symptoms, and outcome were collected.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.475
      Issue No: Vol. 140, No. 7 (2020)
       
  • 466 The relationship between atopic dermatitis and childhood symptoms of
           attention deficit/hyperactivity disorder: A longitudinal cohort study
    • Authors: Y. Lee; N. Tomaszewski, S. Langan, K. Abuabara
      Abstract: The rise in atopic dermatitis (AD) has been paralleled by rising prevalence of attention deficit/hyperactivity disorder (ADHD) in the past few decades, especially in developed and Western countries. Previous research shows a possible association between AD and ADHD, but data have been mixed, likely due to poorly defined phenotypes and heterogeneity in measuring AD and ADHD. Furthermore, most studies have been limited by cross-sectional designs. To test our hypothesis that active and more severe early childhood AD is associated with clinician-diagnosed ADHD at age 7, and hyperactive symptoms as measured by the Strengths and Difficulties Questionnaire (SDQ) at ages 7, 10, 13, and 16 years, we analyzed data from the Avon Longitudinal Study of Parents and Children (ALSPAC), a large population-based birth cohort from the UK.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.474
      Issue No: Vol. 140, No. 7 (2020)
       
  • 465 Real-world risk of new onset inflammatory bowel disease among
           psoriasis patients exposed to interleukin 17 inhibitors
    • Authors: S. Wright; A. Alloo, A. Strunk, A. Garg
      Abstract: Information on real-world risk of inflammatory bowel disease (IBD) among interleukin-17 inhibitor (IL-17i) exposed psoriasis patients is limited. We aimed to compare the risk of new-onset IBD in IL-17i exposed and unexposed psoriasis patients. This was a retrospective cohort analysis using the IBM Explorys analytics platform. We calculated and compared the incidence rates (IR) of IBD in 1,821 psoriasis patients with IL-17i exposure and 213,060 psoriasis patients without IL-17i exposure. Crude 6-month IBD incidence was 0.16% (3/1,821) among psoriasis patients exposed to any IL-17i, 0.24% (3/1,246) among those exposed to secukinumab alone, and 0.11% (239/213,060) among those unexposed.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.473
      Issue No: Vol. 140, No. 7 (2020)
       
  • 464 Treatment patterns of psoriasis by medical providers and disease
           severity in US women
    • Authors: M. Tajalli; T. Li, M. Hasan, A.M. Drucker, A. Qureshi, E. Cho
      Abstract: Studies on treatment patterns of psoriasis are valuable to evaluate how efficiently psoriasis patients are treated and facilitate improved outcomes for these patients. In the Nurses’ Health Study II which includes data for 116,430 female nurses, 2107 women reported to have a diagnosis of psoriasis made by a medical provider. Psoriasis Screening Tool-2 (PST-2) was sent to all of these women. PST-2 is a validated diagnostic tool for psoriasis, which also queries participants for age at disease diagnosis, treatments, type of psoriasis lesions, body surface area involved, and the provider who made the diagnosis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.472
      Issue No: Vol. 140, No. 7 (2020)
       
  • 463 Plasma levels of IL-6 and CRP predict risk of developing psoriasis in
           US women
    • Authors: M. Tajalli; T. Li, E. Cho, A. Qureshi, T. Vance
      Abstract: There are no specific biological markers that predict psoriasis. We conducted a prospective case-control study to explore whether certain inflammatory and hormonal biomarkers were associated with risk of psoriasis. In the Nurses’ Health Study, 32,826 women provided blood samples from 1989 to 1990. We included incident cases of psoriasis among women free of diabetes, cardiovascular disease, and cancer at the time of blood collection. The diagnosis of psoriasis was made from1998 to 2008. Controls consisted of women who had specific biomarker data and did not have psoriasis diagnosis.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.471
      Issue No: Vol. 140, No. 7 (2020)
       
  • 462 Examination of current staging systems in cutaneous squamous cell
           carcinoma
    • Authors: K. Severson; S.A. Ederaine, J. Montoya, R. Butterfield, N. Zhang, J. Besch-Stokes, A. Hughes, S. Ochoa, S. Nelson, D.J. DiCaudo, A. Sekulic, M.R. Pittelkow, A. Mangold
      Abstract: Introduction: Cutaneous squamous cell carcinoma (cSCC) is the second most prevalent skin cancer with an estimated incidence of 700,000 cases in the United States. Our research examines the AJCC 7th, AJCC 8th and BWH staging systems. Methods: 949 retrospective cases of cSCC were randomly sampled between 2009 to 2016 from the Mayo Clinic Enterprise and stratified to mirror the US population. Chart review was performed to collect patient demographics, tumor characteristics, and outcomes. Results: The mean age was 74.5 (SD 11.8), 65.2% were male, 34.8% were female, and 95.0% were Caucasians.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.470
      Issue No: Vol. 140, No. 7 (2020)
       
  • 461 Emojis in dermatology: Changing the faces in medicine
    • Authors: C. Barber; R. Dellavalle, T. Coolman, V. Ranpariya
      Abstract: Today's modern world is focused around incorporating advancing technologies into day-to-day activities to streamline tasks, and the medical field is no different. The surge of emoticon (emoji) use has flourished in the past decade, with more platforms available for delivery, and more emoticons being created constantly. Emojis have already found purpose in medicine, as proxies for emotions expression, and understanding between providers. Studies thus far involving emojis have integrated them into depression screens and medication instruction handouts.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.469
      Issue No: Vol. 140, No. 7 (2020)
       
  • 460 Clinicopathological features of primary melanoma with TERT promoter
           and BRAF mutations in Chinese Uygur and Han nationality: A retrospective
           study of 63 cases
    • Authors: X. Kang; Q. Li
      Abstract: Objective: To detect TERT promoter and BRAF mutation of 63 primary melanoma patients, and explore the association between these mutations and clinicopathological features. Methods: A total of 63 fixed paraffin-embedded formalin tumor sections were analyzed for TERT promoter and BRAF mutation by PCR amplification and DNA sequencing. Results: Among 63 cases,26(92.9%)cases of Han was ALM predominantly, 22 (64.7%)cases of Uygur was NCSD.25 cases of TERT promoter mutations (40.32%), higher than study of Xue Bai.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.468
      Issue No: Vol. 140, No. 7 (2020)
       
  • 459 Understanding the impact of psoriatic disease on mental health:
           Results from the National Psoriasis Foundation Annual Survey
    • Authors: G. Gondo; J. Domire, J.F. Merola, A. Gottlieb, S. Bell
      Abstract: An estimated 8.3 million individuals in the United States live with psoriasis. The NPF has conducted a national survey of the psoriatic disease community for nearly 20 years. One objective of the survey is to understand the impact of psoriatic disease on the mental health of individuals living with psoriasis and psoriatic arthritis. Methods: A nationwide survey of a random sample of individuals with psoriatic disease was conducted online and by telephone. The participant pool was stratified by gender, disease type, and balanced by the estimated population of individuals with psoriatic disease by geographic region.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.467
      Issue No: Vol. 140, No. 7 (2020)
       
  • 458 Effects of text message reminders on data collection in a pragmatic
           study
    • Authors: B. Hefele; D. Shin, J. Gelfand, S. Baez Vanderbeek
      Abstract: Missing data is a large problem facing the clinical research community. Identifying factors that lead to increased response rate can lead to more complete data collection, robust analyses and subsequently highly impactful evidence-based and patient centered recommendations. The LITE Study is a pragmatic trial comparing the effectiveness of home versus office based phototherapy for the treatment of psoriasis. Patients complete quality of life surveys within a 7-day window every 4 weeks for 24-weeks on their mobile phones and are compensated $20 per submission.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.466
      Issue No: Vol. 140, No. 7 (2020)
       
  • 457 Teledermatology service quality improvement at the Southeast
           Veterans’ Integrated Service Network
    • Authors: T.M. DeGrazia; S. Chisolm, S. De La Feld, H. Yeung, S.C. Chen
      Abstract: The Southeast Veterans’ Integrated Service Network (VISN7) has a robust teledermatology program providing services to community outpatient centers in Georgia, South Carolina and Alabama. Despite rapid growth of teledermatology across the Veterans Health Administration, there has not been standardization in teledermatology practices. To provide optimal, standardized consult readings among dermatologists at the VISN7, we examined adherence to 5 mutually agreed upon best practices. Quarterly from July 2018-August 2019, each physician was assigned a month to randomly review teledermatology consult cases and to evaluate if they adhered to the best practices.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.465
      Issue No: Vol. 140, No. 7 (2020)
       
  • 456 Sleep disturbances in chronic pruritic dermatoses are associated with
           increased C-reactive protein levels
    • Authors: S.P. Patel; R. Khanna, J. Choi, K. Williams, S. Kwatra
      Abstract: Pruritus is a common symptom that can significantly reduce quality of life often through sleep disruption. We sought to investigate the features of disturbed sleep in patients with chronic pruritic dermatoses and test the hypothesis that increased systemic inflammation may serve as a biomarker for impaired sleep in these patients. We conducted a cross-sectional analysis of the National Health and Nutrition Examination Survey data. Odds ratios (OR), 95% confidence intervals (95%CI), and p-values using logistic regression were calculated weighted to the US Census data.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.464
      Issue No: Vol. 140, No. 7 (2020)
       
  • 455 Dupilumab monotherapy improves signs, symptoms and quality of life in
           adult and adolescent patients with erythrodermic atopic dermatitis
    • Authors: A. Shabbir; A.S. Paller, B. Lockshin, M.J. Cork, M. Morren, Y. Kataoka, Z. Chen, A.B. Rossi, Y. Lu
      Abstract: Erythrodermic atopic dermatitis (E-AD; ≥90% body surface area [BSA] affected and baseline [BL] erythema score ≥1 from Global Individual Signs Score) is often difficult to treat and highly disturbs patient (pt) physical/mental health. We report pooled efficacy data from dupilumab (DPL) monotherapy trials in adult and adolescent pts with E-AD. In 16-week DPL trials, 136 adults and adolescents with E-AD received DPL 300mg weekly (qw; n=38); DPL 200mg (adolescents: BL weight
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.463
      Issue No: Vol. 140, No. 7 (2020)
       
  • 454 Association of night shift work with chronic spontaneous urticaria and
           effect modification by circadian dysfunction among workers
    • Authors: M. Shen; Y. Xiao, Y. Huang, X. Chen
      Abstract: Night shift work is a social and biological stress that has been identified as a risk factor for many diseases. The study aimed to investigate the association of night shift work with chronic spontaneous urticaria (CSU), and the effect modification by circadian dysfunction. A cross-sectional survey was conducted among workers in China. Exposure was measured as the history and duration of rotating night shift work. Circadian dysfunction was indicated by excessive daytime sleepiness (EDS). CSU was diagnosed by dermatologists in the field survey.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.462
      Issue No: Vol. 140, No. 7 (2020)
       
  • 453 Association of processed meat and sodium intake with atopic dermatitis
           in adults: A pooled analysis of three cross-sectional studies in China and
           validation in NHANES 2005-2006
    • Authors: M. Shen; Y. Xiao, Y. Li, X. Chen
      Abstract: Intake of processed food that contains high content of salt and nitrite are associated with poorer health outcomes. A recent study indicated that salt could stimulate Th2 cell differentiation in vitro experiment, and might link atopic dermatitis (AD). The study aimed to investigate intakes of processed meat, pickles, and estimated salt and nitrite with adult AD in three population-based studies among rural residents, civil servants, and workers in China. Intakes of processed meat and pickles during the past year were inquired through a food frequency questionnaire.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.461
      Issue No: Vol. 140, No. 7 (2020)
       
  • 452 Limited readability and accuracy of patient facing google search
           results for Hidradenitis Suppurativa
    • Authors: N. Shukla; J. Jia, N. Admassu, E. Linos, H. Naik
      Abstract: Hidradenitis Suppurativa (HS) is a chronic inflammatory disease that affects ∼1% of the Western population. In the face of median diagnostic delays of over 13 years, patients often turn to online resources for information. Our aim was to characterize the readability and accuracy of HS information available online. On January 6, 2020, 3 independent investigators searched Google for ‘Hidradenitis Suppurativa,’ generating 1,060000 results. Advertisements, videos and materials that did not include the terms ‘Hidradenitis Suppurativa’ were excluded.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.460
      Issue No: Vol. 140, No. 7 (2020)
       
  • 451 Clinical image identification of basal cell carcinoma and pigmented
           nevus based on convolutional neural networks
    • Authors: X. He; Y. Wang, S. Zhao, X. Chen
      Abstract: This paper constructs a intelligent diagnosis model for basal cell carcinoma (BCC) and pigmented nevus (PN) based on convolutional neural networks. First, a clinical image data set of skin diseases dominated by Chinese people was constructed, in which the classification performance of five mainstream CNN models (Resnet50, InceptionV3, InceptionResNetV2, DenseNet121, Xception) was evaluated. Then, the optimal CNN classification model was compared with 30 dermatologists on 100 real-world BCC and PN patient cases.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.459
      Issue No: Vol. 140, No. 7 (2020)
       
  • 450 Risk of inflammatory bowel disease in patients with atopic dermatitis-
           a population based cohort study
    • Authors: M. Syed; D. Shin, J. Wan, J. Gelfand
      Abstract: Atopic dermatitis (AD), a chronic inflammatory skin condition, has known associations with other allergic and non-allergic comorbidities, suggesting that it may be a systemic disorder with impacts beyond the skin. Less is known about AD and its association with other chronic inflammatory conditions such as inflammatory bowel disease (IBD). We aimed to quantify the risk of IBD among patients with AD, stratified by age compared to the general population,after adjusting for traditional risk factors.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.458
      Issue No: Vol. 140, No. 7 (2020)
       
  • 449 Characterizing a cohort of pediatric patients with hidradenitis
           suppurativa
    • Authors: K. Babbush; A. Waldman, M. Ghias, A. Nosrati, K. Pacific, D. Lee, S. Cohen
      Abstract: Hidradenitis suppurativa (HS) is a chronic inflammatory skin condition characterized by recurrent, painful inflammatory nodules that form extensive draining abscesses and dermal tracts. Median age of onset in females is 19 years and in males is 23 years. A small number of cases in pediatrics have been reported, and less than 2% of patients have disease onset before 11 years. There is a paucity of knowledge about pediatric HS. An IRB-approved retrospective chart review of pediatric patients treated at the Montefiore Hidradenitis Suppurativa Treatment Center (HSTC) from January 2015 to August 2019 identified 72 individuals.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.457
      Issue No: Vol. 140, No. 7 (2020)
       
  • 448 Challenges of securing insurance approval for oral tofacitinib
           treatment of alopecia areata: A multi-institutional retrospective review
    • Authors: S. Desai; K. Lo, V. Nambudiri, C. Villa, A. LaChance, R. Vleugels
      Abstract: Alopecia areata (AA) – an inflammatory hair loss disorder affecting 4.5 million Americans – can lead to psychological distress and negatively impact quality of life (QOL). While oral tofacitinib, approved for rheumatoid arthritis therapy in the US in 2012, has demonstrated efficacy in treating AA,1,2 including in clinical trials, it is not yet approved by the Food and Drug Administration (FDA) for this indication. We reviewed patient records from 2 academic medical centers and identified 38 patients with AA in whom insurance approval was sought for oral tofacitinib.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.456
      Issue No: Vol. 140, No. 7 (2020)
       
  • 445 Diagnostic delay in hidradenitis suppurativa not associated with
           severity
    • Authors: P.C. Morss; A.B. Kimball, M. Porter
      Abstract: The natural history of hidradenitis suppurativa (HS) is not well understood. An association between diagnostic delay with increased HS severity has been suggested.1 An IRB-approved retrospective study of 193 patients seen in an HS clinic over 4 years was performed. Data was collected at initial visit using a standardized questionnaire, including patient self-reported age at disease onset and diagnosis, gender, and race/ethnicity, and physician assessment of disease severity by Hurley stage and HS-PGA.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.453
      Issue No: Vol. 140, No. 7 (2020)
       
  • 444 Cutaneous langerhans cell histiocytosis in adults: Clinical features,
           disease course, and management among patients treated at the Dana-Farber
           Cancer Institute between 2003-2017
    • Authors: A. Bui; A. Laga Canales, E. Jacobsen, N.R. LeBoeuf
      Abstract: Langerhans Cell Histiocytosis (LCH) is a rare inflammatory neoplasm originating from myeloid dendritic cells1. Empiric treatment for LCH in adults has been described as a “roulette wheel” as there lack guidelines on staging, surveillance, and management1. Patients with cutaneous LCH may develop systemic disease and secondary hematologic malignancies; however, incidence and time to progression remains to be studied2. This is a retrospective cohort study of 31 patients (median age 53) with cutaneous LCH at the Dana-Farber Cancer Institute between 2003 to 2017 and literature review.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.452
      Issue No: Vol. 140, No. 7 (2020)
       
  • 443 Lichen planopilaris and frontal fibrosing alopecia: A possible link
           with rosacea
    • Authors: L. Burns; B. De Souza, E. Flynn, D. Hagigeorges, M. Senna
      Abstract: Lichen planopilaris (LPP) and its clinical variant, frontal fibrosing alopecia (FFA), are primary cicatricial alopecias. The etiopathogenesis remains unknown, however the 2 conditions primarily affect post-menopausal, Caucasian women. An increased incidence of rosacea has been identified in this patient cohort, with rates of 34-62% in FFA1,2 compared to 1.5-10% in the general European population3. In this cross-sectional cohort study, we evaluate the incidence, subtype, and severity of rosacea in 66 patients presenting for evaluation of LPP/FFA.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.451
      Issue No: Vol. 140, No. 7 (2020)
       
  • 442 Frontal fibrosing alopecia: Utilization of the Lichen Planopilaris
           Activity Index to assess treatment outcomes
    • Authors: A. Nguyen; R. Freese, D. Windenburg, I. Wallender, R. Farah, M.K. Hordinsky
      Abstract: Frontal fibrosing alopecia (FFA) is a primary cicatricial alopecia which affects the frontotemporal scalp and face. The etiology of FFA is thought to be related to environmental exposures and a genetic susceptibility. FFA is considered to be a relatively new disease; there is a paucity of knowledge surrounding its pathogenesis, treatment and prognosis. Because FFA is histologically similar to lichen planopilaris (LPP), we chose to characterize long-term outcomes of our FFA patients using the LPP Activity Index (LPPAI), a validated scoring system introduced in 2010 which combines symptoms, signs and progression.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.450
      Issue No: Vol. 140, No. 7 (2020)
       
  • 441 Penile calciphylaxis: A retrospective analysis of ten cases
    • Authors: C. Gabel; T. Chakrala, R. Shah, M.J. Danesh, A.C. Walls, S.U. Nigwekar, P.I. Song, D. Kroshinsky
      Abstract: Background: Calciphylaxis is a disorder of vascular calcium deposition leading to ischemia and skin necrosis. Rarely, there may be penile involvement, which has not been well documented. Here we describe ten cases of penile calciphylaxis to assess risk factors and mortality. Methods: A case series of patients with penile calciphylaxis at Massachusetts General Hospital and Beth Israel Deaconess Medical Center was generated through retrospective chart review of patients with a diagnosis of calciphylaxis between January 2001 to December 2018.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.449
      Issue No: Vol. 140, No. 7 (2020)
       
  • 439 Characteristics of patients with generalized pustular psoriasis
           compared to those with psoriasis vulgaris: A claims database study
    • Authors: N. Kotowsky; S.R. Feldman, E.M. Garry, W.C. Valdecantos, R. Gao, A.K. Golembesky
      Abstract: Generalized pustular psoriasis (GPP) is a rare and severe systemic disease characterized by recurrent acute flares consisting of disseminated erythematous skin rash with sterile neutrophil-filled pustules. There is little real-world evidence characterizing patients (Pts) with GPP. This study examined demographics, comorbidities, and concurrent medication use for Pts with GPP compared to those with psoriasis vulgaris (PsO) in a real-world setting. GPP or PsO Pts were identified if they had ≥1 inpatient or ≥2 outpatient ICD-10 diagnosis codes (L40.1 or L40.0, respectively) separated by 30 to 365 days.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.447
      Issue No: Vol. 140, No. 7 (2020)
       
  • 438 Characteristics of patients with palmoplantar pustulosis compared to
           those with psoriasis vulgaris: A claims database study
    • Authors: N. Kotowsky; S.R. Feldman, E.M. Garry, W.C. Valdecantos, R. Gao, A.K. Golembesky
      Abstract: Palmoplantar pustulosis (PPP) is a chronic, debilitating, and painful inflammatory skin disease, characterized by localized sterile pustules on the hands and feet. There is little real-world evidence characterizing patients (Pts) with PPP. This study examined demographics, comorbidities, and concurrent medication use for PPP Pts compared to those with psoriasis vulgaris (PsO) in a real-world setting. PPP or PsO Pts were identified if they had ≥1 inpatient or ≥2 outpatient ICD-10 diagnosis codes (L40.3 or L40.0, respectively) separated by 30 to 365 days.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.446
      Issue No: Vol. 140, No. 7 (2020)
       
  • 437 Risk of second primary malignancies in Kaposi Sarcoma: A U.S.
           population-based study
    • Authors: B. Nardone; M. Nasca, W. Liszewski, D.P. West, G. Micali
      Abstract: An increased risk for second primary malignancies (SPMs) has been reported with Kaposi Sarcoma (KS), however the risk for SPMs in those who have HIV-Related Death (HIV-RD) vs those with no HIV-RD (noHIV-RD) has not been reported. The aim of this study was to determine the risk of SPMs for KS by comparing noHIV-RD vs HIV-RD. The Surveillance, Epidemiology, and End Results (SEER) database (2000-2016) was searched to detect all patients with a primary KS who survived> 2 mo after diagnosis (histology codes 9140/3).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.445
      Issue No: Vol. 140, No. 7 (2020)
       
  • 436 Deep analysis of autoimmune blistering disease subtype and HLA
           associations in Chinese population
    • Authors: Y. Sun; H. Liu, F. Zhang
      Abstract: Autoimmune blistering diseases (AIBDs) are a group of rare acquired blistering skin diseases, which are divided into five major subtypes based on the clinical appearance and pathology: pemphigus diseases, bullous pemphigoid (BP), epidermolysis bullosa acquisita (EBA), dermatitis herpetiformis (DH) and Linear IgA bullous dermatosis (LigA). Current understanding has been greatly increased by genetic investigations mainly focus on the HLA in various populations. We have conducted the HLA association studies on different subtypes of AIBDs in Chinese population by using Next-generation (NGS) based HLA typing methods.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.444
      Issue No: Vol. 140, No. 7 (2020)
       
  • 435 The association between tobacco smoke exposure during childhood and
           adolescence and atopic dermatitis activity and severity
    • Authors: N.A. Al-Alusi; F.D. Ramirez, S. Langan, K. Abuabara
      Abstract: Atopic dermatitis (AD) is the most common chronic inflammatory skin disease worldwide, yet the triggers of disease activity and persistence are still not completely understood. Tobacco smoke exposure (TSE) is an environmental factor that deserves particular attention because of its known effects on humoral and cellular immunity. The objective of our study was to investigate the extent to which TSE during childhood and adolescence is associated with AD activity and severity. Data were obtained from the Avon Longitudinal Study of Parents and Children, a longitudinal population-based cohort from the UK with 10,518 individuals followed from birth through adolescence.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.443
      Issue No: Vol. 140, No. 7 (2020)
       
  • 434 Association of skin response in erythema and sclerosis with survival
           in chronic graft-versus-host disease
    • Authors: L.X. Baker; M. Byrne, P. Martin, S. Lee, H. Chen, M. Jagasia, E. Tkaczyk
      Abstract: Skin is the most commonly affected organ in chronic graft-versus-host disease (cGVHD). Though causing significant morbidity, skin is not a common direct cause of mortality. We report survival associations of complete (CR) vs partial (PR) and no cutaneous response (NR). Using the cGVHD Consortium, a prospective observational study of cGVHD patients from 2007-2017 at 9 centers, we studied patients who were enrolled =3 months (prevalent) after diagnosis of cutaneous cGVHD and within 3 years of transplant.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.442
      Issue No: Vol. 140, No. 7 (2020)
       
  • 433 The practice of dermatology: Comparison of patient characteristics and
           healthcare delivery between dermatology and non-dermatology providers
    • Authors: A.Y. Chen; I. Singh, A.J. Uy, A.W. Armstrong
      Abstract: A thorough understanding of the overall landscape of dermatological care is important for improving patient access and healthcare policies. In this study, we sought to compare patient characteristics and healthcare delivery methods between dermatology providers and non-dermatology providers. We conducted a cross-sectional study using the National Ambulatory Medical Care Survey from 2015-2016. During this period, there were 43.5 million visits (weighted) annually to dermatology providers, which accounted for 4.6% of all ambulatory visits across specialties.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.441
      Issue No: Vol. 140, No. 7 (2020)
       
  • 432 Ichthyosis affects mental health in adults and children: A
           cross-sectional study
    • Authors: Q. Sun; I. Ren, T. Zaki, K. Maciejewski, K. Choate
      Abstract: The impact of ichthyosis on mental health is unknown. The purpose of our cross-sectional study is to determine the relationship between quality of life (QoL) and mental health in adults and children with ichthyosis. To do this, we surveyed 181 patients (128 adults and 53 children) from the National Registry for Ichthyosis and Related Skin Types using age-appropriate QoL, depression and anxiety questionnaires. Spearman’s correlation and logistic regression models determined the QoL and mental health relationship.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.440
      Issue No: Vol. 140, No. 7 (2020)
       
  • 431 Differences in dermatologic care for acne vulgaris patients with
           autism
    • Authors: R. Fort; J. Sciacca Kirby, S. Maczuga
      Abstract: Atypical sensory processing occurs commonly in individuals with Autism Spectrum Disorder (ASD) and may impact the care these patients receive for skin conditions like acne vulgaris. This is a retrospective cohort study of acne-specific medication and outpatient utilization and costs from MarketScan© database (2015-2017) for continuously enrolled 11 to 18-year-old patients. People with acne were identified based on at least one instance of ICD-9/ICD-10 code (706.1/L70.0), then assigned to a cohort with ASD based on at least one instance of ICD-9/ICD-10 code (299, F84) or the cohort without ASD based on random selection of age and sex at a 10:1 ratio.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.439
      Issue No: Vol. 140, No. 7 (2020)
       
  • 430 Crohn's disease prevalence prior to and following hidradenitis
           suppurativa diagnosis
    • Authors: M. Rosales Santillan; K. Savage, M. Porter, R. Parker, M. Simon, A.B. Kimball
      Abstract: Previous studies have shown differing results on the prevalence of Crohn’s disease in HS [1,2]. Our study used a large claims database to determine the prevalence of Crohn’s disease in HS. The Arcadia.io database includes insurance claims and visit-level data from> 34,000,000 discrete lives. The database was searched for individuals with a diagnosis of Crohn's disease within the general patient cohort. Patients with>1 coded diagnosis of HS (ICD-9:705.83 or ICD-10:L73.2) in a clinical setting at any time were identified.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.438
      Issue No: Vol. 140, No. 7 (2020)
       
  • 429 Risk of keratinocyte carcinoma among patients with hidradenitis
           suppurativa
    • Authors: S. Ashrafzadeh; Y. Kim, G. Peters, H. Lee, M. Asgari
      Abstract: Recent studies have implicated the role of the Notch pathway in keratinocyte carcinomas (KCs). Notch signaling is often upregulated in basal cell carcinoma (BCC) but downregulated in squamous cell carcinoma (SCC). Hidradenitis suppurativa (HS) is an inflammatory disorder of the apocrine glands whose pathogenesis has also been associated with downregulation of Notch signaling. Given the role of Notch in the pathogenesis of HS and KCs, we hypothesized that HS patients could be at decreased risk for BCC and increased risk for SCC.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.437
      Issue No: Vol. 140, No. 7 (2020)
       
  • 428 Increased incidence of non-melanoma skin cancers among rural kidney
           transplant recipients
    • Authors: H. Maredia; J. Motter, A. Massie, D. Segev, M. Loss
      Abstract: Skin cancers are the most common malignancy in kidney transplant (KT) recipients. Current data supports an uneven distribution of dermatological care towards urbanized areas, leading to limited access in rural settings. Patients in rural areas thus may have more difficulty in obtaining routine skin screening after transplant. We hypothesized that there would be higher risk of skin cancers after transplant among rural KT recipients and sought to examine rural-urban differences in skin cancer incidence in KT recipients in the US.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.436
      Issue No: Vol. 140, No. 7 (2020)
       
  • 427 Leukocytoclastic vasculitis with and without IgA deposition is
           associated with renal damage: A case-control study
    • Authors: E.M. Leland; Y. Semenov, B. Kaffenberger, K. Williams, J. Alhariri, S. Kwatra
      Abstract: Leukocytoclastic vasculitis (LCV) is a small vessel vasculitis presenting with palpable purpura. Direct immunofluorescence (DIF) is routinely performed on skin biopsy to identify IgA deposition, which is thought to contribute to greater frequency of renal comorbidity associated with IgA vasculitis. Knowledge of characteristics between LCV with and without IgA deposition is limited. This study characterized presentations of LCV with and without IgA. Charts identified in the JHH pathologic database system with biopsy confirmed LCV between 2009-2018 were reviewed.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.435
      Issue No: Vol. 140, No. 7 (2020)
       
  • 426 Review of acne evaluation and management published in clinical
           practice guidelines for gender-affirming hormone therapy
    • Authors: A.M. Cartron; J. Zampella, H. Yeung
      Abstract: Acne is a known side effect for transgender patients receiving testosterone for gender affirmation. Clinical practice guidelines and policy statements for transgender patients exist to provide evidence-based recommendations. There is a knowledge gap regarding optimal treatment of acne in transgender patients receiving masculinizing hormone therapy. We conducted a narrative review of guidelines for the management of transgender patients. Clinical practice guidelines for hormone therapy in transgender patients that were published in English were identified using Google, UpToDate, and PubMed with the search terms “transgender” and “acne” and “guidelines” or “hormones” or “testosterone.” Of 12 guidelines reviewed, 7 mentioned acne as a potential adverse effect from testosterone therapy.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.434
      Issue No: Vol. 140, No. 7 (2020)
       
  • 425 Characteristics associated with physician-identified melanomas vs
           personally-identified melanomas
    • Authors: D.J. Lewis; D. Shin, M.E. Ming
      Abstract: Melanomas identified by patients or their friends or family are thicker than those identified by physicians, indicating that they are likely found at a later point in their development. If patients were able to identify their melanomas earlier, their prognosis potentially could be improved. We sought to determine what clinical characteristics distinguish melanomas found by physicians (“physician-identified melanomas”) vs those found by patients or their friends or family (“personally-identified melanomas”).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.433
      Issue No: Vol. 140, No. 7 (2020)
       
  • 424 Quality of life in patients with facial cutaneous lupus erythematosus
    • Authors: J.S. Concha; D. Yan, A. Ravishankar, C. Bax, R. Borucki, V. Werth
      Abstract: Lesions on exposed areas are known to have a significant impact in the quality of life (QoL) of patients. This study aims to compare the QoL of patients with cutaneous lupus erythematosus on the face (FCLE) versus patients without facial lesions, and to determine whether lesion activity (erythema, scale) and damage (pigmentation, scarring) on the face have an impact on QoL. This is a cross-sectional study of a database of CLE patients seen at the University of Pennsylvania. Patients with a diagnosis of CLE, have available Cutaneous Lupus Erythematosus Disease Area and Severity Index (CLASI) assessments, and who were able to complete the Skindex-29+3 questionnaire on the initial visit were included.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.432
      Issue No: Vol. 140, No. 7 (2020)
       
  • 422 Influence of climate on pediatric alopecia areata flares
    • Authors: E.A. George; L. Castelo-Soccio, E. Putterman, H. Kuhn, B. Prindaville, A. Qureshi, E. Cho
      Abstract: We recently found that the frequency of alopecia areata (AA) flares follows seasonal patterns, with the highest number of flares during fall and the lowest number during summer, and explored the relationship between climate aspects and AA flares. Information on date of disease onset, first flare and second flare was extracted from the medical records of 336 pediatric AA patients (ages 1 to 12), totaling 520 episodes of AA in Philadelphia, Pennsylvania. Frequency of flares in each month was calculated.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.430
      Issue No: Vol. 140, No. 7 (2020)
       
  • 421 Does the provision of melanoma genetic risk information change
           preventative behavior'
    • Authors: J.M. Kilgour; X. Hu, A. Fogel, P. Jaju, K. Sarin
      Abstract: With the rise of direct-to-consumer testing, personalized genetic risk information is accessible to the public. It is unclear, however, what impact the provision of this information has on preventative behaviors for melanoma. Stanford dermatology clinic patients were offered genetic sequencing. Genetic risk scores (GRS) were calculated using 15 risk SNPs identified in prior GWAS studies. Participants were randomized to receive their GRS immediately or delayed for 6 months. All participants received standard sunscreen advice.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.429
      Issue No: Vol. 140, No. 7 (2020)
       
  • 420 Melanoma in pregnancy in California, 1994-2015: A population-based
           study
    • Authors: M. Kiuru; Q. Li, G. Zhu, J. Terrell, K. Beroukhim, T. Keegan
      Abstract: Melanoma is the most common malignancy during pregnancy. There is continued debate regarding the impact of pregnancy on the prognosis of melanoma. Prior studies have shown mixed results and the number of recent large population-based studies are limited, especially from the United States. To address this question, we used the California Cancer Registry data linked with Patient Discharge Data and Ambulatory Surgery Center Data to identify female patients, ages 15-44 years, diagnosed with melanoma in 1994-2015, including those who were pregnant.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.428
      Issue No: Vol. 140, No. 7 (2020)
       
  • 419 Clinical characteristics and quality of life burden in aquagenic
           pruritus: A global questionnaire-based study
    • Authors: P. Aggarwal; Y. Semenov, E. Boozalis, J. Choi, T. Le, K. Williams, S. Kwatra
      Abstract: Aquagenic pruritus (AP) is a rare pruritic dermatosis characterized by intense itching following water exposure and often results in patients avoiding water contact. Limited epidemiologic studies describe the characteristics of AP and its impact on quality of life. Our objective was to characterize the demographics, itch, and quality of life of AP patients using a descriptive cross-sectional study design. An anonymous, online Qualtrics survey was developed and delivered to 1,829 patients via a Facebook AP support group in July 2019.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.427
      Issue No: Vol. 140, No. 7 (2020)
       
  • 418 A randomized control trial of IT prevention messages for SMM
    • Authors: N.E. Admassu; L.D. Morrison, R.M. Chen, N. Shukla, E. Linos
      Abstract: Despite increased risks of indoor tanning (IT) and skin cancer among sexual minority men (SMM), IT prevention messages to date have only focused on teens and young women. We created and tested seven IT prevention messages on Facebook to assess ad engagement and impact on IT knowledge and intentions among SMM. Facebook users identifying as men 18 years and older, with LGBT interests, residing in six states with high rates of IT were randomized to see prevention messages in their newsfeed. Control group users saw their regular newsfeed.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.426
      Issue No: Vol. 140, No. 7 (2020)
       
  • 417 Adherence to topical therapy for atopic dermatitis: Barriers and
           facilitators
    • Authors: A. Bazen; A. Sevagamoorthy, F.K. Barg, J. Takeshita
      Abstract: In spite of continuing advances in therapeutic options particularly for more severe atopic dermatitis (AD), topical treatments remain a mainstay of the therapeutic regimen for all patients. However, adherence to topical therapies remains low. Increasing adherence to topical therapies is an important contributor to improving outcomes for patients with AD. In order to better understand the barriers to and facilitators of adhering to topical therapies for AD from the patient perspective, we performed semi-structured interviews of 44 adults with AD.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.425
      Issue No: Vol. 140, No. 7 (2020)
       
  • 416 Impact of prior authorizations on dermatology patients at a large
           academic center
    • Authors: L. Guo; V. Nambudiri
      Abstract: Prescription drugs in dermatology have been increasingly subject to prior authorization (PA) policies, adding burdens and barriers to both patients and providers. However, few studies have formally characterized their effect on patients. Newly prescribed dermatology medications commonly requiring PAs in 2017 at a large academic institution were retrospectively reviewed (n=515). For each prescription, whether PA was required, PA decision if applicable, and whether follow-up clinic visit occurred within 6 months were assessed.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.424
      Issue No: Vol. 140, No. 7 (2020)
       
  • 415 The epidemiology of genital warts in the United States
    • Authors: W. Liszewski; P. Singh
      Abstract: Dermatologists frequently diagnose and treat genital warts. Given the expanded approval of the HPV vaccine for individuals up to the age of 45, there may be an opportunity for dermatologists to recommend the vaccine in previously ineligible individuals. To better understand the burden of genital warts in the United States—and which individuals would benefit from it--we analyzed the demographics, sexual history, and HPV vaccination status of individuals with and without a history of genital warts in the 2013-2016 National Health and Nutrition Examination Survey.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.423
      Issue No: Vol. 140, No. 7 (2020)
       
  • 414 Incidence of depression among patients with hidradenitis suppurativa
    • Authors: S. Wright; A. Strunk, A. Garg
      Abstract: Information on the risk of depression among patients with hidradenitis suppurativa (HS) is limited with much of the existing data consisting of cross-sectional studies lacking a control group. We aimed to compare risk of new-onset depression in patients with HS to that of controls without HS, and to determine clinical characteristics associated with depression among HS patients. This was a retrospective cohort analysis of 49,280 adult and 3,042 pediatric HS patients and matched controls identified using the IBM Explorys analytics platform.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.422
      Issue No: Vol. 140, No. 7 (2020)
       
  • 413 Wildfire-associated air pollution impacts clinic visits for itch and
           atopic dermatitis
    • Authors: R. Fadadu; B. Grimes, J. Balmes, M. Wei
      Abstract: With climate change, the frequency and intensity of wildfires are expected to increase, leading to episodes of poor air quality that could exacerbate patients’ pre-existing dermatoses. To assess the effects of exposure to wildfire-associated air pollution on skin, we investigated if the 2018 California Camp Fire led to detectable increases in clinic visits for atopic dermatitis (AD) or itch at dermatology clinics 175 miles from the origin of the fire. We collected data from 2015-2019 on particulate matter (PM2.5) concentration and smoke plume density in San Francisco and the number of outpatient dermatology visits for AD or itch symptoms at an academic medical center.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.421
      Issue No: Vol. 140, No. 7 (2020)
       
  • 412 Comparisons of oral corticosteroid treatment patterns for
           toxicodendron dermatitis
    • Authors: M. Butt; S. Maczuga, A. Flamm
      Abstract: Toxicodendron Dermatitis (TD) is a common form of allergic contact dermatitis that affects millions of Americans every year. While some cases remain mild and can be managed with over-the-counter remedies, many patients experience significant pruritus from the eruptions and can require systemic medical intervention to ease the symptoms. The objective of this study is to evaluate medical claims associated with TD outpatient visits and compare healthcare utilization outcomes depending on the days’ supply of the initial oral corticosteroid prescription.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.420
      Issue No: Vol. 140, No. 7 (2020)
       
  • 411 Implementation of a consultative teledermatology mobile application in
           Veterans Affairs
    • Authors: S. Peracca; A. Fonseca, A. Grenga, G. Jackson, H. King, J. Chapman, J. Whited, M. Weinstock, D.H. Oh
      Abstract: To facilitate consultative asynchronous teledermatology, Veterans Affairs (VA) introduced a mobile application at 3 sites. We evaluated the initial implementation process using a mixed methods multiple case study approach to assess readiness to implement the intervention in each facility, as well as the facilitators, barriers and contextual factors. Data sources included: 1) Interviews and an organizational readiness to change (ORC) survey conducted at one site; 2) Interviews at all sites with groups of physicians, nurses, administrators, and information technology (IT) professionals; and 3) Implementation documentation from the operational partner.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.419
      Issue No: Vol. 140, No. 7 (2020)
       
  • 410 Frequency and influence of “Not Relevant” responses on the
           Dermatology Life Quality Index among adults with atopic dermatitis in the
           United States
    • Authors: J. Barbieri; Z. Chiesa Fuxench, J. Takeshita
      Abstract: Introduction: Recent data among adults with psoriasis raise concerns about frequent “not relevant” responses on the widely used Dermatology Life Quality Index (DLQI), which may result in underestimation of disease burden. To examine whether these concerns extend to other skin diseases, we evaluated the frequency and influence of “not relevant” responses on the DLQI among adults with atopic dermatitis in the United States Methods: A cross-sectional analysis was conducted using data from the Atopic Dermatitis in America survey.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.418
      Issue No: Vol. 140, No. 7 (2020)
       
  • 409 Dupilumab prevents flares in adults with moderate-to-severe atopic
           dermatitis in a 52-week, randomized, controlled, phase 3 trial
    • Authors: J.F. Merola; R. Sidbury, A. Wollenberg, Z. Chen, A. Zhang, B. Shumel, A.B. Rossi
      Abstract: Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by flares, defined by US and EU guidelines as acute, clinically significant worsening of signs and symptoms of AD requiring therapeutic intervention. Flare prevention is a hallmark of long-term disease control in AD. Here, we report the effect of dupilumab treatment for 52 weeks on flare prevention in adults with moderate-to-severe AD from the LIBERTY AD CHRONOS trial (NCT02260986). In this trial, 740 patients (pts) with moderate-to-severe AD were randomized 3:1:3 to subcutaneous dupilumab 300 mg once weekly, dupilumab 300 mg every 2 weeks (q2w), or placebo (PBO); all pts also received a standardized regimen of medium potency topical corticosteroids (TCS).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.417
      Issue No: Vol. 140, No. 7 (2020)
       
  • 408 Long-term treatment with dupilumab minimizes use of systemic
           immunosuppressants as rescue medications in adults with moderate-to-severe
           atopic dermatitis
    • Authors: L.A. Beck; A. Blauvelt, L. Sher, I. Hussain, A.B. Rossi, Z. Chen, B. Shumel
      Abstract: LIBERTY AD OLE (NCT01949311) is a phase 3, open-label extension study that evaluates the long-term safety and efficacy of dupilumab in adults with moderate-to-severe atopic dermatitis (AD) who were previously enrolled in dupilumab clinical trials. We report the proportion of patients (pts) who required systemic rescue medications. All pts who received ≥ 1 dose of dupilumab and required ≥ 1 concomitant rescue medication during the study were included in this analysis. Rescue treatments included systemic corticosteroids, nonsteroidal systemic immunosuppressants (ISS), and phototherapy, administered at the investigator’s discretion to treat intolerable AD symptoms or to manage serious intercurrent conditions.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.416
      Issue No: Vol. 140, No. 7 (2020)
       
  • 407 High burden of patient-reported ocular disorders and symptoms in
           adults with moderate-to-severe atopic dermatitis
    • Authors: J. Weyne; A. Blauvelt, M. de Bruin-Weller, E. Prens, P. Asbell, D. Sierka, Z. Chen, B. Shumel
      Abstract: Patients with atopic dermatitis (AD) have a higher risk of conjunctivitis and other ocular surface disorders than the general population, and the incidence of ocular complications increases with AD severity. Here, we report the burden of ocular disorders and symptoms prior to treatment initiation in adults with moderate-to-severe AD with inadequate response to topical corticosteroids enrolled in LIBERTY AD CHRONOS (NCT02260986), a randomized, placebo-controlled, phase 3 trial of dupilumab. CHRONOS enrolled 740 patients after a 35-day screening period.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.415
      Issue No: Vol. 140, No. 7 (2020)
       
  • 405 Association between cutaneous squamous cell carcinoma primary tumor
           anatomic site, laterality, and odds of invasion in the United States
    • Authors: S. Ghanian; T. Li, J. Han, A. Qureshi, J. Walker, E. Cho
      Abstract: Cutaneous squamous cell carcinoma (cSCC) is the second most common skin cancer that is associated with ultraviolet radiation exposure and immunosuppression. This study aims to examine the association between cSCC primary tumor anatomic site, laterality, and odds of tumor invasion in females in the Nurses’ Health Study (NHS) and Nurses’ Health Study II (NHS2) and in males in the Health Professionals Follow-Up Study (HPFS). A pathology review of cSCC cases from the NHS (n= 4,867), NHS2 (n=1,165), and HPFS (n=2,779) was conducted to investigate the association between primary tumor anatomic site and odds of tumor invasion.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.413
      Issue No: Vol. 140, No. 7 (2020)
       
  • 404 Physicians’ ability to determine culprit drug in SJS/TEN and
           areas for improvement
    • Authors: D. Li; G. Romar, R. Foreman, S.J. Divito
      Abstract: Stevens-Johnson syndrome (SJS)/Toxic Epidermal Necrolysis (TEN) is a severe drug reaction causing mucocutaneous desquamation with high morbidity and mortality. Central to management is identifying the culprit drug to stop disease progression and recurrence. Little is known about physicians’ approach and ability to identify the culprit drug and consequences of misidentification, especially given better understanding of disease pathogenesis and tools like the algorithm of drug causality for epidermal necrolysis (ALDEN).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.412
      Issue No: Vol. 140, No. 7 (2020)
       
  • 402 Framing application site discomfort as an efficacy signal improves
           willingness to continue use of topical medications
    • Authors: A.M. Bashyam; E.J. Masicampo, S.R. Feldman
      Abstract: One obstacle to topical therapy adherence is patient perception of treatment safety and efficacy. Medications for atopic dermatitis (AD) can cause application site discomfort (burning or stinging), leading patients to discontinue therapy. However, counseling patients can frame the sensation as a positive signal of efficacy. This study assessed differences in patient willingness to tolerate application site discomfort when application site sensation is forewarned and framed as an efficacy signal.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.410
      Issue No: Vol. 140, No. 7 (2020)
       
  • 401 Health care expenditures of psoriatic patients with and without
           comorbid depression
    • Authors: N.L. Tamashunas; D. DeMeo, J. Scott
      Abstract: While the association between psoriasis and depression is well-established, studies investigating the impact of depression on health care expenditures are limited to adult psoriasis patients with commercial insurance or Medicare. We hypothesize that psoriatic patients with depression have higher health care expenditures than psoriatic patients without depression. This retrospective cross-sectional study pooled data from the Medical Expenditure Panel Survey (MEPS), a nationally representative sample of the non-institutionalized United States population, from 2007 to 2015.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.409
      Issue No: Vol. 140, No. 7 (2020)
       
  • 400 Rates of BCC relative to SCC are higher in younger patients,
           especially females
    • Authors: T.M. Lukowiak; L. Aizman, A. Perz, J. Etkzorn, C. Miller, T. Shin, J. Sobanko, C. Giordano, W. Higgins, M.P. Lee
      Abstract: In the United States (U.S.) data on keratinocyte carcinoma (KC) epidemiology has been derived from the Medicare population1 or regional data2. In Medicare patients, the ratio of BCC:SCC in 2012 was approximately 1:1 in patients ≥ 65 years old1, but in a regional study of Mayo clinic patients, the ratio of BCC:SCC was higher in patients less than 40 years old2. Defining sex and age-specific variations in KC epidemiology could inform targeted public health messaging. The aim of this study was to assess BCC:SCC ratios from adults (≥18 years old) between 2012-2016.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.408
      Issue No: Vol. 140, No. 7 (2020)
       
  • 399 Risk of hospitalization due to infection in patients with psoriasis: A
           population-based cohort study using the UK Clinical Practice Research
           Datalink
    • Authors: Z.Z. Yiu; R. Parisi, M. Lunt, R. Warren, C. Griffiths, S. Langan, D. Ashcroft
      Abstract: Psoriasis is associated with multiple comorbidities and treated with systemic therapies that may increase the risk of serious infections. Our objective was to determine whether patients with psoriasis have a higher risk of hospitalization due to infection. We performed a cohort study of adult patients (≥18 years of age ) with psoriasis delineated from the UK Clinical Practice Research Datalink (CPRD GOLD) and linked to Hospital Episode Statistics (HES) and national mortality records between 01/04/2003 and 31/12/2016.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.407
      Issue No: Vol. 140, No. 7 (2020)
       
  • 398 Incidence and predictors of acne among transgender patients treated
           with masculinizing hormone therapy
    • Authors: N. Thoreson; C. Grasso, D. King, C. Shen, E. Dommasch
      Abstract: Transgender patients treated with masculinizing hormone therapy (MHT) for gender affirmation may be at higher risk of developing acne following hormone initiation. We sought to examine the incidence and severity of acne in patients treated with MHT and factors which may predict development of acne. We conducted a retrospective cohort study using electronic medical records from a community health center which provides care to the LGBTQIA+ population for patients who started MHT between 2014 and 2017 (n=1,054).
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.406
      Issue No: Vol. 140, No. 7 (2020)
       
  • 397 Long-term effectiveness of spironolactone treatment for women with
           acne
    • Authors: V. Garg; J. Choi, W. James, J. Barbieri
      Abstract: Background: Use of oral spironolactone for acne has grown over the past decade. However, data on the effectiveness and safety of spironolactone is limited to small randomized trials and retrospective studies with limited follow-up. Objective: To characterize the long-term use of spironolactone for women with acne. Methods: We conducted a retrospective chart review of adult women with acne who were treated with spironolactone at an academic medical center from 2008 to 2018. We evaluated the proportion of patients whose acne had cleared at 3, 6, 12, and 24 months.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.405
      Issue No: Vol. 140, No. 7 (2020)
       
  • 396 The global burden of nonmelanoma skin cancers from 1990 to 2017
    • Authors: W. Zhang; X. Shen, Y. Wang, Y. Lan, X. Yang, Y. Gu, J. Duan, H. Lu
      Abstract: Nonmelanoma skin cancers (NMSCs) consist of two major subtypes, basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). Information about the NMSCs epidemiology limited, here we provide an estimate of the global burden of NMSCs. Based on the GBD study 2017, we analysed detailed data on NMSCs of incidence, mortality and the disability-adjusted life-years (DALYs) metric between 1990-2017. Estimated annual percentage changes (EAPCs) in NMSCs age standardized incidence, mortality and DALYs rate (ASIR, ASMR and ASDR), were calculated to quantify the temporal trends in ASR.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.404
      Issue No: Vol. 140, No. 7 (2020)
       
  • 395 Burden of malignant skin melanoma in Worldwide, 1990-2017: An analysis
           of the Global Burden of Disease Study 2017
    • Authors: W. Zhang; W. Zeng, Z. Liu, X. Dong, H. Luo, Z. Zheng, Z. He, T. Ye, H. Lu
      Abstract: Numerous population-based studies have documented high morbidity and mortality of malignant skin melanoma (MSM) in light-skinned people. To update on the global epidemiology of melanoma, we provide an estimate of the burden of melanoma using data from the Global Burden of Disease (GBD) Study 2017. We analysed detailed data on melanoma epidemiology (case number and age-standardized rate (ASR) ) including incidence, mortality and the disability-adjusted life-years (DALYs) metric between 1990-2017, derived from the GBD study in 2017.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.403
      Issue No: Vol. 140, No. 7 (2020)
       
  • 394 Top 50 dermatology influencers on Twitter
    • Authors: T.M. Wells; M. Maymone, C.W. Rundle, R. Dellavalle
      Abstract: Social media has reshaped the interactions between patients and health care professionals. The increased visibility and accessibility of practitioners on social media has led to emergence “influencers” in all fields of medicine, including dermatology. The growing presence of dermatology influencers on social media prompts questioning of the identities of those influencers. In this study we sought to identify the top 50 dermatology influencers on Twitter, characterize who they are, and compare their average academic impact with their social media influence.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.402
      Issue No: Vol. 140, No. 7 (2020)
       
  • 393 Survival and glycemic control in patients with squamous cell carcinoma
    • Authors: S.A. Ederaine; J. Dominguez, J. Harvey, A. Mangold, C. Cook, H. Kosiorek, M. Buras, K. Coppola, P. Verona, N. Karlin
      Abstract: This study examined the impact of diabetes mellitus (DM) on survival in head and neck and cutaneous squamous cell cancer (SCC) patients, and the impact of SCC on glycemic control in DM. Patients with newly diagnosed SCC (n=95) were identified from the institutional cancer registry between the years 2007-2017 and matched to 95 patients with SCC without DM based on age, gender, and year of SCC diagnosis. Data on DM, cancer therapies and laboratory results were culled from the electronic medical record.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.401
      Issue No: Vol. 140, No. 7 (2020)
       
  • 392 Temporal trends in the incidence of metastatic melanoma and
           utilization of immunotherapy in the United States
    • Authors: T. Bhat; C. Herbosa, W. Hodges, S. Kwatra, K. Reynolds, D. Chen, L. Cornelius, Y. Semenov
      Abstract: The aim of this study was to investigate temporal trends in the incidence of metastatic melanoma (MM) and immunotherapy utilization as well as the impact of geography on these trends. We performed a retrospective cohort study of 12,544 patients with MM extracted from Truven MarketScan®, a national private insurance claims database, from 2011-2015. Immunotherapy use was documented for claims for ipilimumab, nivolumab, or pembrolizumab within 180 days of MM diagnosis. Geographic regions were defined by standard US census regions, with the Northeast set as reference.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.400
      Issue No: Vol. 140, No. 7 (2020)
       
  • 391 Antihypertensives and risk of melanoma and keratinocyte carcinoma: A
           systematic review and meta-analysis
    • Authors: O.G. Cohen; M. Taylor, S.H. Giordano, S. Langan, D.J. Margolis, M.R. Wehner
      Abstract: There are many papers on the association between antihypertensive drugs and skin cancers, with conflicting results. Three recent meta-analyses on this topic have included different articles and produced different outcomes. Additionally, several new papers were not included in prior meta-analyses, which could impact the conclusions. We conducted a systematic review and random-effects meta-analysis to evaluate the most contemporary evidence on antihypertensives and basal cell carcinoma (BCC), squamous cell carcinoma (SCC), and melanoma.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.399
      Issue No: Vol. 140, No. 7 (2020)
       
  • 390 Atopic dermatitis and risk of major neuropsychiatric disorders: A
           population-based cohort study
    • Authors: J. Wan; D. Shin, M. Syed, K. Abuabara, J. Gelfand
      Abstract: Atopic dermatitis (AD) is a chronic skin disease now recognized to have systemic inflammatory effects which may include neuro-immunological abnormalities that are increasingly implicated in neuropsychiatric disorders. Although AD has been previously associated with anxiety, depression, and attention-deficit/hyperactivity disorder (ADHD), longitudinal studies of both children and adults are scarce. We conducted a cohort study using a U.K. population-based electronic health records database to examine the association between AD and several major psychiatric and neurodevelopmental disorders in both children and adults.
      Citation: Journal of Investigative Dermatology 140, 7 (2020)
      PubDate: 2020-07
      DOI: 10.1016/j.jid.2020.03.398
      Issue No: Vol. 140, No. 7 (2020)