Subjects -> MEDICAL SCIENCES (Total: 8690 journals)
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MEDICAL SCIENCES (2415 journals)            First | 3 4 5 6 7 8 9 10 | Last

Showing 1201 - 1400 of 3562 Journals sorted alphabetically
Journal of Evaluation In Clinical Practice     Hybrid Journal   (Followers: 6)
Journal of Evidence-Based Healthcare     Open Access   (Followers: 1)
Journal of Evidence-Based Integrative Medicine     Open Access   (Followers: 18)
Journal of Evidence-Based Medicine     Partially Free   (Followers: 4)
Journal of Exercise Science & Fitness     Open Access   (Followers: 29)
Journal of Experimental and Clinical Anatomy     Open Access   (Followers: 2)
Journal of Family and Community Medicine     Open Access   (Followers: 3)
Journal of Family Medicine and Primary Care     Open Access   (Followers: 11)
Journal of Foot and Ankle Research     Open Access   (Followers: 6)
Journal of Forensic Science and Research     Open Access   (Followers: 2)
Journal of Gandaki Medical College-Nepal     Open Access  
Journal of Generic Medicines     Hybrid Journal   (Followers: 2)
Journal of Geographical Sciences     Hybrid Journal   (Followers: 1)
Journal of Global Antimicrobial Resistance     Hybrid Journal   (Followers: 3)
Journal of Hand Therapy     Hybrid Journal   (Followers: 19)
Journal of Head & Neck Physicians and Surgeons     Open Access   (Followers: 2)
Journal of Health & Medical Informatics     Open Access   (Followers: 62)
Journal of Health and Biological Sciences     Open Access   (Followers: 1)
Journal of Health Design     Open Access   (Followers: 1)
Journal of Health Economics and Outcomes Research     Open Access   (Followers: 1)
Journal of Health Promotion and Behavior     Open Access  
Journal of Health Research and Reviews     Open Access  
Journal of Health Science and Medical Research     Open Access  
Journal of Health Science Research     Open Access  
Journal of Health Sciences     Open Access   (Followers: 1)
Journal of health sciences     Open Access  
Journal of Health Sciences / Sağlık Bilimleri Dergisi     Open Access  
Journal of Health Sciences and Medicine     Open Access  
Journal of Health Sciences and Medicine     Open Access   (Followers: 6)
Journal of Health Sciences and Surveillance System     Open Access  
Journal of Health Sciences Scholarship     Open Access  
Journal of Health Specialties     Open Access  
Journal of Health Studies     Open Access  
Journal of Healthcare Informatics Research     Hybrid Journal   (Followers: 1)
Journal of Heavy Metal Toxicity and Diseases     Open Access  
Journal of Helminthology     Hybrid Journal   (Followers: 2)
Journal of Herbs Spices & Medicinal Plants     Hybrid Journal  
Journal of HIV for Clinical and Scientific Research     Open Access   (Followers: 2)
Journal of Hospital Medicine     Hybrid Journal   (Followers: 11)
Journal of Huazhong University of Science and Technology [Medical Sciences]     Hybrid Journal  
Journal of Human Hypertension     Hybrid Journal   (Followers: 3)
Journal of Human Rhythm     Open Access  
Journal of Human Transcriptome     Open Access  
Journal of Ideas in Health     Open Access  
Journal of Inflammation     Open Access   (Followers: 2)
Journal of Inflammation Research     Open Access  
Journal of Injury and Violence Research     Open Access   (Followers: 6)
Journal of Innovation in Health Informatics     Open Access   (Followers: 17)
Journal of Institute of Medicine     Open Access  
Journal of Insulin Resistance     Open Access   (Followers: 1)
Journal of Interactional Research in Communication Disorders     Hybrid Journal   (Followers: 5)
Journal of Interferon & Cytokine Research     Hybrid Journal   (Followers: 3)
Journal of International Medical Research     Open Access   (Followers: 3)
Journal of Interventional Medicine     Open Access   (Followers: 1)
Journal of Investigative Medicine     Hybrid Journal   (Followers: 3)
Journal of Islamabad Medical & Dental College     Open Access   (Followers: 2)
Journal of Istanbul Faculty of Medicine     Open Access  
Journal of Karnali Academy of Health Sciences     Open Access   (Followers: 1)
Journal of Kathmandu Medical College     Open Access   (Followers: 1)
Journal of King Abdulaziz University : Medical Sciences     Open Access   (Followers: 2)
Journal of Laboratory Medicine     Hybrid Journal   (Followers: 27)
Journal of Laryngology and Voice     Open Access   (Followers: 11)
Journal of Lasers in Medical Sciences     Open Access  
Journal of Law, Medicine & Ethics     Hybrid Journal   (Followers: 28)
Journal of Legal Medicine     Hybrid Journal   (Followers: 7)
Journal of Limb Lengthening & Reconstruction     Open Access  
Journal of Lumbini Medical College     Open Access   (Followers: 1)
Journal of Mahatma Gandhi Institute of Medical Sciences     Open Access  
Journal of Manipulative and Physiological Therapeutics     Hybrid Journal   (Followers: 6)
Journal of Manmohan Memorial Institute of Health Sciences     Open Access   (Followers: 1)
Journal of Marine Medical Society     Open Access  
Journal of Materials Science : Materials in Medicine     Hybrid Journal   (Followers: 4)
Journal of Maternal and Child Health     Open Access  
Journal of Mechanics in Medicine and Biology     Hybrid Journal  
Journal of Medical and Biological Engineering     Hybrid Journal   (Followers: 4)
Journal of Medical and Biomedical Sciences     Open Access   (Followers: 2)
Journal of Medical Case Reports     Open Access   (Followers: 1)
Journal of Medical Cases     Open Access   (Followers: 6)
Journal of Medical Colleges of PLA     Full-text available via subscription  
Journal of Medical Disorders     Open Access  
Journal of Medical Economics     Hybrid Journal   (Followers: 8)
Journal of Medical Education and Curricular Development     Open Access   (Followers: 6)
Journal of Medical Ethics     Partially Free   (Followers: 27)
Journal of Medical Ethics and History of Medicine     Open Access   (Followers: 19)
Journal of Medical Humanities     Hybrid Journal   (Followers: 21)
Journal of Medical Hypotheses and Ideas     Open Access  
Journal of Medical Imaging and Health Informatics     Full-text available via subscription   (Followers: 1)
Journal of Medical Investigation and Practice     Open Access  
Journal of Medical Laboratory and Diagnosis     Open Access  
Journal of Medical Law and Ethics     Full-text available via subscription   (Followers: 17)
Journal of Medical Microbiology     Full-text available via subscription   (Followers: 6)
Journal of Medical Sciences     Open Access  
Journal of Medical Sciences     Open Access  
Journal of Medical Screening     Hybrid Journal   (Followers: 6)
Journal of Medical Signals and Sensors     Open Access   (Followers: 3)
Journal of Medical Society     Open Access  
Journal of Medical Systems     Hybrid Journal  
Journal of Medical Toxicology     Hybrid Journal   (Followers: 6)
Journal of Medical Ultrasound     Open Access   (Followers: 2)
Journal of Medicinal Botany     Open Access  
Journal of Medicinal Chemistry     Hybrid Journal   (Followers: 207)
Journal of Medicine     Open Access   (Followers: 1)
Journal of Medicine and Biomedical Research     Open Access   (Followers: 1)
Journal of Medicine and Philosophy     Hybrid Journal   (Followers: 9)
Journal of Medicine and the Person     Hybrid Journal  
Journal of Medicine in Scientific Research     Open Access  
Journal of Medicine in the Tropics     Open Access  
Journal of Medicine Research and Development     Open Access   (Followers: 3)
Journal of Medicine, Physiology and Biophysics     Open Access   (Followers: 5)
Journal of Medicines Development Sciences     Open Access   (Followers: 1)
Journal of Metabolomics & Systems Biology     Open Access   (Followers: 2)
Journal of Mind and Medical Sciences     Open Access   (Followers: 1)
Journal of Molecular Medicine     Hybrid Journal   (Followers: 11)
Journal of Movement Disorders     Open Access   (Followers: 2)
Journal of Multidisciplinary Research in Healthcare     Open Access   (Followers: 2)
Journal of Muscle Research and Cell Motility     Hybrid Journal   (Followers: 1)
Journal of Nanotechnology in Engineering and Medicine     Full-text available via subscription   (Followers: 6)
Journal of Nanotheranostics     Open Access   (Followers: 1)
Journal of Natural Medicines     Hybrid Journal  
Journal of Natural Science, Biology and Medicine     Open Access   (Followers: 3)
Journal of Nature and Science of Medicine     Open Access   (Followers: 4)
Journal of Negative and No Positive Results     Open Access  
Journal of Nepalgunj Medical College     Open Access  
Journal of Neurocritical Care     Open Access  
Journal of Neurodegenerative Diseases     Open Access   (Followers: 2)
Journal of Neurorestoratology     Open Access  
Journal of Neuroscience and Neurological Disorders     Open Access  
Journal of Nobel Medical College     Open Access  
Journal of Obesity and Bariatrics     Open Access   (Followers: 2)
Journal of Occupational Health     Open Access  
Journal of Occupational Therapy Education     Open Access   (Followers: 12)
Journal of Ocular Biology, Diseases, and Informatics     Hybrid Journal  
Journal of Oral Biology and Craniofacial Research     Full-text available via subscription  
Journal of Oral Health and Craniofacial Science     Open Access  
Journal of Orofacial Sciences     Open Access  
Journal of Otorhinolaryngology, Hearing and Balance Medicine     Open Access   (Followers: 1)
Journal of Ovarian Research     Open Access  
Journal of Ozone Therapy     Open Access  
Journal of Palliative Medicine     Hybrid Journal   (Followers: 47)
Journal of Paramedical Sciences & Rehabilitation     Open Access  
Journal of Parkinsonism and Restless Legs Syndrome     Open Access   (Followers: 2)
Journal of Parkinson’s Disease and Alzheimer’s Disease     Open Access   (Followers: 1)
Journal of Participatory Medicine     Open Access  
Journal of Patan Academy of Health Sciences     Open Access  
Journal of Pathogens     Open Access   (Followers: 1)
Journal of Patient Experience     Open Access  
Journal of Patient Safety and Risk Management     Hybrid Journal   (Followers: 2)
Journal of Patient-Centered Research and Reviews     Open Access  
Journal of Patient-Reported Outcomes     Open Access  
Journal of Periodontal Research     Hybrid Journal  
Journal of Personalized Medicine     Open Access   (Followers: 3)
Journal of Pest Science     Hybrid Journal   (Followers: 1)
Journal of Pharmaceutical Policy and Practice     Open Access   (Followers: 4)
Journal of Physiobiochemical Metabolism     Hybrid Journal   (Followers: 2)
Journal of Physiology-Paris     Hybrid Journal   (Followers: 2)
Journal of Pioneering Medical Sciences     Open Access  
Journal of Postgraduate Medicine     Open Access  
Journal of Pregnancy     Open Access   (Followers: 4)
Journal of Prevention & Intervention Community     Hybrid Journal   (Followers: 7)
Journal of Preventive Medicine and Public Health     Open Access  
Journal of Primary Prevention     Hybrid Journal   (Followers: 7)
Journal of Prosthodontic Research     Full-text available via subscription   (Followers: 1)
Journal of Prosthodontics     Hybrid Journal   (Followers: 2)
Journal of Receptor, Ligand and Channel Research     Open Access   (Followers: 1)
Journal of Regenerative Medicine     Partially Free   (Followers: 4)
Journal of Research in Medical Sciences     Open Access   (Followers: 2)
Journal of Science and Applications : Biomedicine     Open Access   (Followers: 1)
Journal of Science and Technology (Ghana)     Open Access   (Followers: 3)
Journal of Scientific Innovation in Medicine     Open Access  
Journal of Scientific Perspectives     Open Access   (Followers: 1)
Journal of Sensory Studies     Hybrid Journal   (Followers: 4)
Journal of Shaheed Suhrawardy Medical College     Open Access  
Journal of Shoulder and Elbow Arthroplasty     Open Access  
Journal of Sleep Disorders : Treatment & Care     Hybrid Journal   (Followers: 10)
Journal of South American Earth Sciences     Hybrid Journal   (Followers: 5)
Journal of Spinal Cord Medicine     Hybrid Journal   (Followers: 5)
Journal of Spinal Disorders & Techniques     Hybrid Journal   (Followers: 2)
Journal of Sports Medicine and Allied Health Sciences : Official Journal of the Ohio Athletic Trainers Association     Open Access   (Followers: 1)
Journal of Stem Cell Therapy and Transplantation     Open Access   (Followers: 1)
Journal of Stomal Therapy Australia     Full-text available via subscription   (Followers: 1)
Journal of Strength and Conditioning Research     Hybrid Journal   (Followers: 77)
Journal of Substance Use     Hybrid Journal   (Followers: 15)
Journal of Surgical Academia     Open Access   (Followers: 1)
Journal of Surgical and Clinical Research     Open Access  
Journal of Surgical Case Reports     Open Access  
Journal of Surgical Education     Full-text available via subscription   (Followers: 3)
Journal of Surgical Technique and Case Report     Open Access  
Journal of Systemic Therapies     Full-text available via subscription   (Followers: 3)
Journal of Taibah University Medical Sciences     Open Access  
Journal of Telemedicine and Telecare     Hybrid Journal   (Followers: 12)
Journal of The Academy of Clinical Microbiologists     Open Access  
Journal of the American Association for Laboratory Animal Science     Full-text available via subscription   (Followers: 9)
Journal of the American College of Certified Wound Specialists     Hybrid Journal   (Followers: 2)
Journal of the American College of Clinical Wound Specialists     Hybrid Journal   (Followers: 2)
Journal of the American Medical Directors Association     Hybrid Journal   (Followers: 5)
Journal of the American Medical Informatics Association : JAMIA     Hybrid Journal   (Followers: 36)
Journal of the American Podiatric Medical Association     Full-text available via subscription   (Followers: 7)
Journal of the Anatomical Society of India     Full-text available via subscription  
Journal of the Anus, Rectum and Colon     Open Access  
Journal of The Arab Society for Medical Research     Open Access  

  First | 3 4 5 6 7 8 9 10 | Last

Similar Journals
Journal Cover
Journal of Health Sciences and Medicine
Number of Followers: 0  

  This is an Open Access Journal Open Access journal
ISSN (Print) 2549-7561
Published by Universitas Udayana Homepage  [70 journals]
  • Cover and Table of Contents

    • Authors: Sagung Chandra Yowani
      Abstract:    
      PubDate: 2018-02-01
      Issue No: Vol. 1, No. 2 (2018)
       
  • Writing Guidance and Cover Belakang

    • Authors: Sagung Chandra Yowani
      Abstract:    
      PubDate: 2018-02-01
      Issue No: Vol. 1, No. 2 (2018)
       
  • Shiga Like Toxin 1 (STX-1) Detection From Escherichia coli O157:H7 Local
           Isolates

    • Authors: I W. Suardana, K.J. P. Pinatih, D. A. Widiasih
      Pages: 1 - 3
      Abstract: Shiga-like toxin (Stx) otherwise known as verotoxin and verocytotoxin is a toxin produced by some strains of Escherichia coli particularly by strain O157: H7. This toxin is an AB5 toxin type which is known to have similarities with the toxins produced by Shigella dysenteriae . Stx from E. coli  O157: H7 can be distinguished into stx 1 and stx 2. Stx1is usually associated with most outbreaks and detrimental sporadic cases of illness in humans. In this research, we observed the titer of Shiga toxin (Stx 1 / VT1) from local isolates isolated from cattle and human feces by using vero toxin Escherichia coli -reverse passive latex agglutination test (VTEC-RPLA) method. The results showed local isolates KL-48 (2) of human origin, SM-25 (1) of cattle feces origin and DS 21 (4) of beef origin positively produced VT1 2 units of titer, meanwhile the isolate SM 7 (1) was negative. Titer of toxin Stx1 produced from local isolates was known to be lower when compared to the control isolate ATCC 43894 with 8 units of titer.
      PubDate: 2018-01-25
      Issue No: Vol. 1, No. 2 (2018)
       
  • Optimization Of Isolation Method Of Carrageenan From Kappaphycuss
           Alvarezii Doty Using Factorial Experimental Design

    • Authors: Ketut Widyani Astuti, Ni Putu Ayu Dewi Wijayanti, I Gusti Ngurah Agung Dewantara Putra, Ni Putu Linda Laksmiani
      Pages: 4 - 7
      Abstract: Kappaphycus alvarezii Doty is a species of red algae ( Rhodophyceae) producing kappa carrageenan. The value of carrageenan will increase if it is able to form thermoreversible gels. The quality of carrageenan is determined by the isolation method which includes several stages, namely soaking, extraction, purification, and drying. This study aims to obtain an optimum isolation method of carrageenan from seaweed  Kappaphycus alvarezii Doty using the factorial experimental design software, Design Expert Version.7.0.0. with the characteristics that meet the standards set by FAO. The seaweed was collected from the sea in Nusa Lembongan, Bali Province. The carrageenan isolation method was optimized by varying three factors, namely the concentration of NaOH (4% and 8%), extraction time (0.5 hour and 3 hours), and precipitation time (0.25 hour and 0.5 hour) in order to obtain 8 formulae which then went through an evaluation of the physical and chemical characteristics of the carrageenan. The physical and chemical characteristics include the yield, viscosity, sulfate content, and ash content, analyzed using Design Expert Version 7.0.0 program to determine the optimum method of isolation. The analysis showed that the optimum method of carrageenan isolation was using NaOH with a concentration of 4.40%, the extraction time of 2.16 hours and the precipitation time of 0.29 hour. The physical and chemical characteristics of carrageenan with the optimum isolation method include the yield of 25.73%, the viscosity of 11.92 cPs, sulfate content of 31.95% and the ash content of 26.16% which have met the standards set by FAO.
      PubDate: 2018-01-25
      Issue No: Vol. 1, No. 2 (2018)
       
  • Rapid Detection Of Methicillin Resistant Staphylococci Using Multiplex PCR
           With Boiling Method For DNA Isolation

    • Authors: Ida Bagus Gede Adiguna Wibawa, Agus Eka Darwinata, Ni Nengah Dwi Fatmawati, Nyoman Sri Budayanti
      Pages: 8 - 10
      Abstract: Methicillin-Resistant Staphylococcus aureus  (MRSA) is Staphylococcus aureus  that has become insusceptible or resistant by methicillin antibiotic types. Rapid identification of MRSA is essential for early initiation of appropriate antimicrobial therapy. The aim of this research is to reduce the cost and time needed for multiplex PCR in rapid detection of MRSA by finding an alternative method for DNA which is boiling method. DNA isolation was performed with boiling method and kit commercial. The kit method takes time approximately 45 minutes while boiling takes only about 12 minutes. PCR result with boiling technique used in DNA isolation formed amplification bands of 16S rRNA, mecA , and nuc  in MRSA and 16S rRNA and nuc  in MSSA. Conclusion can be drawn that boiling method can be used as an alternative method for DNA extraction.
      PubDate: 2018-01-25
      Issue No: Vol. 1, No. 2 (2018)
       
  • Occurrence of Hypoglycemia, Hypokalemia and Hyperkalemia in Diabetic
           Hypertensive Patients Using Insulin and Diuretics

    • Authors: Sarasmita M A, Setyawan E. I., Hendra Jaya A.
      Pages: 11 - 15
      Abstract: Diabetes Mellitus (DM) is a metabolic disease with characteristics of hyperglycemia that occurs due to insulin secretion abnormalities, insulin work or both. DM can cause macrovascular complications such as hypertension. Administration of therapy may lead to hypoglycemic and hypokalemia that may increase the risk of impaired brain and heart. Objective: The purpose of this study to analyze the incidence of hypoglycemia and hypokalemia side effects in patients with diabetic hypertension using antidiabetes and antihypertensives. Method: The study was conducted retrospectively with retrospective patient medical records from January to December 2016. Blood and potassium blood glucose levels were measured as long as the patient was treated. Random and potassium blood glucose levels prior to therapy were compared with after therapy using paired t-test. Result : From the results of the study found 27 patients suffering from diabetic hypertension and get therapy. The value of changes in the patient's random blood glucose level averaged 280.89 mg/dl to 189.15 mg/dl, which met the target of antidiabetic drug therapy, and no patients had hypoglycemia. From 27 patients, as many as 7 people did not get antihypertensive therapy. Three patients had hyperkalemia (serum potassium level > 5 mEq/L). The value of changes in blood potassium levels of patients averaged 4.74 mEq/L to 4.46 mEq/L, where no patients had hypokalemia. Conclusion : the occurrence of hypoglycemia and hyperkalemia were suspected due to side effect of insulin and antihypertensive drugs.
      PubDate: 2018-01-25
      Issue No: Vol. 1, No. 2 (2018)
       
  • The Determinants of Ethical Principle Implementation in Nursing

    • Authors: Ni Putu Emy Darmayanti, Hanny Handiyani, Kuntarti Kuntarti
      Pages: 16 - 22
      Abstract: The determinant of nursing ethics practice is not identified yet. This problem affects the ethical behavior of nurses in performing nursing care. According to that, it was influencing satisfaction and patient well-being. This study aims to identify the determinants of the ethical principles application in nursing. Descriptive, correlation, cross-sectional design was applied, through simple random sampling to 120 nurses. Data collecting used questionnaires, with univariate, bivariate, and multivariate analyzes. Determinants of nursing ethic application principle were job characteristics, nurses knowledge related to nursing ethics, supervision by supervisor, relationship with colleagues, awards, the role of head nurse, and tenure (p = <0,001-0,049). The most dominant variable was job characteristics (p <0,001). These factors need an attention by hospital manager to keep evaluating the other factors in order to improve the application of ethical principles in nursing.
      PubDate: 2018-01-25
      Issue No: Vol. 1, No. 2 (2018)
       
  • In Vitro Evaluation Of Antioxidant Activity Of Flavonoid Compounds From
           Terong Belanda

    • Authors: Ida Ayu Raka Astiti Asih, Ni Made Puspawati, Wiwik Susanah Rita
      Pages: 23 - 26
      Abstract: Terong belanda (Solanum betaceum, cav), is a fruit that has nutrients and vitamins which are essential for health of human body and it is believed to have a potent antioxidant activity. This research aimed to evaluate in vitro antioxidant activity of flavonoid compounds isolated from Terong belanda. In vitro antioxidant activity was evaluated using DPPH method. Identification of flavonoids was done using Infrared and UV–Vis Spectrophotometers. Extraction of ten (10) kg terong belanda fruits with ethanol gave of 126.17 g peels extract, 178.44 g flesh, and 253.11 g seed extracts respectively. Phytochemical test showed both peels, flesh, and seed extracts of Terong belanda contained flavonoids. Antioxidant activity test showed peels, flesh, and seeds extracts exhibited antioxidant activity with IC50 of 68,14 ppm, 621.45 ppm, and 1162.608 ppm respectively. Separation and purification of these three active extracts using column chromatography gave each one isolate which positive flavonoid on phytochemical test. Infrared spectra of each isolate from peels, flesh, and seed were similar and each revelaed to contain the same functional groups (OH alcohol, CH aromatic, C=O, C=C, and CH aliphatic). Analysis of UV-Vis spectra and its spectra with shifting reagents of the peels isolates gave absorption at the wavelength range of 310-330 nm (Bans I ) and 245-275 nm (bands II) due to isoflavones group with a hydroxy group at C6, C7 or C7, C8 and C3', C 4 ' while flesh and seed gave absorption at 300-330 nm (bands I) and 275-295 nm (bands II) that indicated the class of flavanones with hydroxyl groups at C-2', C-5', C-6' and Oglycoside at C7 for flessh and hydroxyl group at C6, C7 or C7, C8 for the seed.
      PubDate: 2018-01-25
      Issue No: Vol. 1, No. 2 (2018)
       
  • Perception Of Contraception Access And Utilization In Teenagers In Senior
           High School No 8 Denpasar, Bali

    • Authors: R. Listyowati, N. M. S. Nopiyani, P. A. Indrayathi
      Pages: 27 - 30
      Abstract: The images of teenagers in the world, especially in South Asia and Southeast Asia have less information about reproductive and sexual health because of the assumption that it is unnecessary even considered harmful if they knew it. It’s not surprise that the teen birth rate is still quite high. previous research in Indonesia on adolescent premarital sexual behavior, obtain a yield of about 25% - 51% of teens have sex before marriage. (Utomo ID 2009; 40 (2) :. 133-46.). Studies in Bali obtain the results of teenage boys in high school (SMA) and junior high school (SMP) more premarital sex (40.3% and 29.4%) than girls (3.6% and 12.5%). Teenage boys in Bali more permissive toward premarital sexual behavior than girls and about 5% of teens have sex before marriage (Faturochman 1992; 1 :. 12-7) Adolescents have a right to a healthy life. They need to have access of the tools that can protect their health, such as condoms, contraception and youth-friendly health services are comprehensive. Governed by Law no. 36/2009 Article 4 and 5, which states that everyone has the same right to gain access of health and this rule does not see marital status. The purpose of this study was to describe the general perception of access and use of contraception among adolescents in the city of Denpasar.
      PubDate: 2018-01-25
      Issue No: Vol. 1, No. 2 (2018)
       
  • DNA Probe Design for Detection Mutation at Codon 315 In katG Gene of
           Mycobacterium Tuberculosis to Real-Time Polymerase Chain Reaction

    • Authors: I Gusti A. A. Santhi Rahmaryani, Ni Kadek Ariani, Dyah Subadrika Warma Dewi, Ni Komang Sasi Ani, Ade Ari Sundari, Kadek Widya Yuli Hartati, Sagung Chandra Yowani
      Pages: 31 - 41
      Abstract: High-level resistance to isoniazid as a first-line tuberculosis drugs can be caused by mutations in codon 315 katG Mycobacterium tuberculosis . Mutation at codon 315 is the most frequent mutation with the highest amino acid variation, compared to other codons in the Mycobacterium tuberculosis katG  gene. Therefore, a specific probe  is required for rapid and proper detection of mutations at codon 315. In this study, the design of a nucleotide sequence probe with TaqMan labeling was performed using Clone Manager Suite 6 software . The mutant probe  obtained was analysed in two stages. The initial analysis is based on the length of the probe  (22-30 bases), Tm (70ºC), %GC (35-65%), not in hairpin  form, dimer  (< 5 bases), runs  and repeat  (' 4 for base A, T, C, and < 3 for base G). Furthermore the final analysis was carried out with no G base in 2 bases at the end of the 5’ probe  and the amount of base C ' G. The study resulted in 260 probe mutants. After the initial analysis, 11 mutant probes were obtained to recognize mutations in the codon of 315 katG Mycobacterioum tuberculosis  genes. The probe  consists of 2 probes  for the S315T mutation, 6 probes for S315N mutation, and 3 probes  for S315V mutation. The criteria of the 11 mutant probes  are 22-23 bases long, Tm 70ºC, % GC 56-63%, 4 dimer , 2 runs , and does not have repeats  and does not form hairpin  at a temperature of 56ºC. Based on the final analysis, 3 mutant probes  were obtained fulfilling the TaqMan probe labeling criteria, namely K315MT1 for specific detection of mutation S'T and K315MN5, then K315MN23 for specific detection of mutation S'N. The conclusion of this study shows that the best mutant nucleotide sequence probes for the detection of mutations at codon of 315 KatG Mycobacterium tuberculosis  genes are 5’-FAM-CC ACC GGC ATC GAG GTC GTA TG-TAMRA-3’; 5’FAM-ATC ACC AAC GGC ATC GAG GTC G-TAMRA-3’; dan 5’FAM-C ACC AAC GGC ATC GAG GTC GTA T-TAMRA-3’. The design of the mutant probe according to the TaqMan probe criterion for real-time PCR was obtained by 3 probes from the 11 selected mutant probes in the initial analysis.
      PubDate: 2018-01-25
      Issue No: Vol. 1, No. 2 (2018)
       
 
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