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Showing 1 - 200 of 3562 Journals sorted alphabetically
16 de Abril     Open Access  
3D Printing in Medicine     Open Access   (Followers: 2)
AADE in Practice     Hybrid Journal   (Followers: 5)
ABCS Health Sciences     Open Access   (Followers: 3)
Abia State University Medical Students' Association Journal     Full-text available via subscription  
ACIMED     Open Access   (Followers: 1)
ACS Medicinal Chemistry Letters     Hybrid Journal   (Followers: 42)
Acta Bio Medica     Full-text available via subscription   (Followers: 2)
Acta Bioethica     Open Access  
Acta Bioquimica Clinica Latinoamericana     Open Access   (Followers: 1)
Acta Facultatis Medicae Naissensis     Open Access  
Acta Informatica Medica     Open Access   (Followers: 1)
Acta Medica (Hradec Králové)     Open Access  
Acta Medica Bulgarica     Open Access  
Acta Medica Colombiana     Open Access   (Followers: 1)
Acta Médica Costarricense     Open Access   (Followers: 2)
Acta Medica Indonesiana     Open Access  
Acta Medica International     Open Access  
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Acta Medica Martiniana     Open Access  
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Acta Medica Saliniana     Open Access  
Acta Scientiarum. Health Sciences     Open Access   (Followers: 1)
Acupuncture & Electro-Therapeutics Research     Full-text available via subscription   (Followers: 6)
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Addiction Science & Clinical Practice     Open Access   (Followers: 8)
Addictive Behaviors Reports     Open Access   (Followers: 8)
Adıyaman Üniversitesi Sağlık Bilimleri Dergisi / Health Sciences Journal of Adıyaman University     Open Access  
Adnan Menderes Üniversitesi Sağlık Bilimleri Fakültesi Dergisi     Open Access  
Advanced Biomedical Research     Open Access  
Advanced Health Care Technologies     Open Access   (Followers: 4)
Advanced Science, Engineering and Medicine     Partially Free   (Followers: 8)
Advances in Bioscience and Clinical Medicine     Open Access   (Followers: 6)
Advances in Clinical Chemistry     Full-text available via subscription   (Followers: 26)
Advances in Life Course Research     Hybrid Journal   (Followers: 8)
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Advances in Medical Sciences     Hybrid Journal   (Followers: 7)
Advances in Medicinal Chemistry     Full-text available via subscription   (Followers: 5)
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Advances in Parkinson's Disease     Open Access  
Advances in Phytomedicine     Full-text available via subscription  
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Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 20)
Advances in Regenerative Medicine     Open Access   (Followers: 3)
Advances in Skeletal Muscle Function Assessment     Open Access  
Advances in Therapy     Hybrid Journal   (Followers: 5)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 14)
Advances in Virus Research     Full-text available via subscription   (Followers: 5)
Advances in Wound Care     Hybrid Journal   (Followers: 11)
Aerospace Medicine and Human Performance     Full-text available via subscription   (Followers: 12)
African Health Sciences     Open Access   (Followers: 3)
African Journal of Biomedical Research     Open Access   (Followers: 1)
African Journal of Clinical and Experimental Microbiology     Open Access   (Followers: 2)
African Journal of Laboratory Medicine     Open Access   (Followers: 2)
African Journal of Medical and Health Sciences     Open Access   (Followers: 2)
African Journal of Trauma     Open Access   (Followers: 1)
Afrimedic Journal     Open Access   (Followers: 2)
Aggiornamenti CIO     Hybrid Journal   (Followers: 1)
AIDS Research and Human Retroviruses     Hybrid Journal   (Followers: 9)
AJOB Primary Research     Partially Free   (Followers: 3)
AJSP: Reviews & Reports     Hybrid Journal  
Aktuelle Ernährungsmedizin     Hybrid Journal   (Followers: 4)
Al-Azhar Assiut Medical Journal     Open Access  
Alexandria Journal of Medicine     Open Access   (Followers: 1)
Allgemeine Homöopathische Zeitung     Hybrid Journal   (Followers: 3)
Alpha Omegan     Full-text available via subscription  
ALTEX : Alternatives to Animal Experimentation     Open Access   (Followers: 3)
Althea Medical Journal     Open Access  
American Journal of Biomedical Engineering     Open Access   (Followers: 13)
American Journal of Biomedical Research     Open Access   (Followers: 2)
American Journal of Biomedicine     Full-text available via subscription   (Followers: 7)
American Journal of Chinese Medicine, The     Hybrid Journal   (Followers: 4)
American Journal of Clinical Medicine Research     Open Access   (Followers: 7)
American Journal of Family Therapy     Hybrid Journal   (Followers: 11)
American Journal of Law & Medicine     Full-text available via subscription   (Followers: 11)
American Journal of Lifestyle Medicine     Hybrid Journal   (Followers: 5)
American Journal of Managed Care     Full-text available via subscription   (Followers: 11)
American Journal of Medical Case Reports     Open Access   (Followers: 1)
American Journal of Medical Sciences and Medicine     Open Access   (Followers: 4)
American Journal of Medicine     Hybrid Journal   (Followers: 48)
American Journal of Medicine and Medical Sciences     Open Access   (Followers: 1)
American Journal of Medicine Studies     Open Access   (Followers: 1)
American Journal of Medicine Supplements     Full-text available via subscription   (Followers: 3)
American Journal of the Medical Sciences     Hybrid Journal   (Followers: 12)
American Journal on Addictions     Hybrid Journal   (Followers: 9)
American medical news     Free   (Followers: 3)
American Medical Writers Association Journal     Full-text available via subscription   (Followers: 5)
Amyloid: The Journal of Protein Folding Disorders     Hybrid Journal   (Followers: 5)
Anales de la Facultad de Medicina     Open Access  
Anales de la Facultad de Medicina, Universidad de la República, Uruguay     Open Access  
Anales del Sistema Sanitario de Navarra     Open Access   (Followers: 1)
Analgesia & Resuscitation : Current Research     Hybrid Journal   (Followers: 6)
Anatolian Clinic the Journal of Medical Sciences     Open Access  
Anatomica Medical Journal     Open Access  
Anatomical Science International     Hybrid Journal   (Followers: 3)
Anatomical Sciences Education     Hybrid Journal   (Followers: 1)
Anatomy     Open Access   (Followers: 1)
Anatomy Research International     Open Access   (Followers: 2)
Angewandte Schmerztherapie und Palliativmedizin     Hybrid Journal  
Angiogenesis     Hybrid Journal   (Followers: 3)
Ankara Medical Journal     Open Access   (Followers: 2)
Ankara Üniversitesi Tıp Fakültesi Mecmuası     Open Access  
Annales de Pathologie     Full-text available via subscription  
Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale     Full-text available via subscription   (Followers: 3)
Annals of African Medicine     Open Access   (Followers: 2)
Annals of Anatomy - Anatomischer Anzeiger     Hybrid Journal   (Followers: 2)
Annals of Bioanthropology     Open Access   (Followers: 4)
Annals of Biomedical Engineering     Hybrid Journal   (Followers: 17)
Annals of Biomedical Sciences     Full-text available via subscription   (Followers: 3)
Annals of Clinical Hypertension     Open Access  
Annals of Clinical Microbiology and Antimicrobials     Open Access   (Followers: 12)
Annals of Family Medicine     Open Access   (Followers: 14)
Annals of Health Research     Open Access  
Annals of Ibadan Postgraduate Medicine     Open Access  
Annals of Medical and Health Sciences Research     Open Access   (Followers: 7)
Annals of Medicine     Hybrid Journal   (Followers: 12)
Annals of Medicine and Surgery     Open Access   (Followers: 7)
Annals of Microbiology     Hybrid Journal   (Followers: 11)
Annals of Nigerian Medicine     Open Access   (Followers: 1)
Annals of Rehabilitation Medicine     Open Access  
Annals of Saudi Medicine     Open Access  
Annals of the New York Academy of Sciences     Hybrid Journal   (Followers: 5)
Annals of The Royal College of Surgeons of England     Full-text available via subscription   (Followers: 3)
Annual Reports in Medicinal Chemistry     Full-text available via subscription   (Followers: 7)
Annual Reports on NMR Spectroscopy     Full-text available via subscription   (Followers: 5)
Annual Review of Medicine     Full-text available via subscription   (Followers: 16)
Anthropological Review     Open Access   (Followers: 23)
Anthropologie et santé     Open Access   (Followers: 5)
Antibiotics     Open Access   (Followers: 9)
Antibodies     Open Access   (Followers: 2)
Antibody Technology Journal     Open Access   (Followers: 1)
Antibody Therapeutics     Open Access  
Anuradhapura Medical Journal     Open Access  
Anwer Khan Modern Medical College Journal     Open Access   (Followers: 2)
Apmis     Hybrid Journal   (Followers: 1)
Apparence(s)     Open Access   (Followers: 1)
Applied Clinical Informatics     Hybrid Journal   (Followers: 3)
Applied Clinical Research, Clinical Trials and Regulatory Affairs     Hybrid Journal  
Applied Medical Informatics     Open Access   (Followers: 12)
Arab Journal of Nephrology and Transplantation     Open Access   (Followers: 1)
Arak Medical University Journal     Open Access  
Archive of Clinical Medicine     Open Access   (Followers: 1)
Archive of Community Health     Open Access   (Followers: 1)
Archives Medical Review Journal / Arşiv Kaynak Tarama Dergisi     Open Access  
Archives of Asthma, Allergy and Immunology     Open Access  
Archives of Medical and Biomedical Research     Open Access   (Followers: 3)
Archives of Medical Laboratory Sciences     Open Access   (Followers: 1)
Archives of Medicine and Health Sciences     Open Access   (Followers: 3)
Archives of Medicine and Surgery     Open Access  
Archives of Trauma Research     Open Access   (Followers: 3)
Archivos de Medicina (Manizales)     Open Access  
ArgoSpine News & Journal     Hybrid Journal  
Arquivos Brasileiros de Oftalmologia     Open Access   (Followers: 1)
Arquivos de Ciências da Saúde     Open Access  
Arquivos de Medicina     Open Access  
Ars Medica : Revista de Ciencias Médicas     Open Access  
ARS Medica Tomitana     Open Access   (Followers: 1)
Art Therapy: Journal of the American Art Therapy Association     Full-text available via subscription   (Followers: 15)
Arterial Hypertension     Open Access   (Followers: 1)
Artificial Intelligence in Medicine     Hybrid Journal   (Followers: 14)
Artificial Organs     Hybrid Journal   (Followers: 1)
ASHA Leader     Open Access  
Asia Pacific Family Medicine     Open Access   (Followers: 1)
Asia Pacific Journal of Clinical Nutrition     Full-text available via subscription   (Followers: 11)
Asia Pacific Journal of Clinical Trials : Nervous System Diseases     Open Access  
Asian Bioethics Review     Full-text available via subscription   (Followers: 3)
Asian Biomedicine     Open Access   (Followers: 2)
Asian Journal of Cell Biology     Open Access   (Followers: 5)
Asian Journal of Health     Open Access   (Followers: 3)
Asian Journal of Medical and Biological Research     Open Access   (Followers: 4)
Asian Journal of Medical and Pharmaceutical Researches     Open Access   (Followers: 1)
Asian Journal of Medical Sciences     Open Access   (Followers: 2)
Asian Journal of Scientific Research     Open Access   (Followers: 3)
Asian Journal of Transfusion Science     Open Access   (Followers: 1)
Asian Medicine     Hybrid Journal   (Followers: 5)
Asian Pacific Journal of Cancer Prevention     Open Access  
ASPIRATOR : Journal of Vector-borne Disease Studies     Open Access  
Astrocyte     Open Access  
Atención Familiar     Open Access  
Atención Primaria     Open Access   (Followers: 1)
Atti della Accademia Peloritana dei Pericolanti - Classe di Scienze Medico-Biologiche     Open Access  
Audiology - Communication Research     Open Access   (Followers: 10)
Auris Nasus Larynx     Full-text available via subscription  
Australian Coeliac     Full-text available via subscription   (Followers: 1)
Australian Family Physician     Full-text available via subscription   (Followers: 3)
Australian Journal of Medical Science     Full-text available via subscription   (Followers: 1)
Autopsy and Case Reports     Open Access  
Avicenna     Open Access   (Followers: 3)
Avicenna Journal of Clinical Medicine     Open Access  
Avicenna Journal of Medicine     Open Access   (Followers: 1)
Bangabandhu Sheikh Mujib Medical University Journal     Open Access   (Followers: 1)

        1 2 3 4 5 6 7 8 | Last

Journal Cover
Annals of Microbiology
Journal Prestige (SJR): 0.479
Citation Impact (citeScore): 2
Number of Followers: 11  
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1590-4261 - ISSN (Online) 1869-2044
Published by Springer-Verlag Homepage  [2352 journals]
  • New approaches to modeling Staphylococcus aureus inactivation by
    • Authors: Bahman Soleimanzadeh; Atefeh Amoozandeh; Mehdi Shoferpour; Mahmoud Yolmeh
      Pages: 313 - 319
      Abstract: Ultrasound (US) is an effective technology to inactivate vegetative microorganisms in foods. In this study, the effect of amplitude levels (0.4, 7.5, and 37.5), duty cycles (0.3:0.7 s, 0.7:0.3 s, and 0.9: 0.1 s) and time (0, 2, 4, 6, 8, 10, 12, and 14 days) of US on inactivation of Staphylococcus aureus were investigated. In addition, genetic algorithm-artificial neural network (GA-ANN) and adaptive neuro-fuzzy inference system (ANFIS) models were used to predict inactivation of S. aureus. The GA-ANN and ANFIS were fed with three inputs of amplitude levels, duty cycles, and time. The inactivation rate of S. aureus was increased by increasing the amplitude levels, and the best inactivation was obtained at a 37.5 μm amplitude for which the S. aureus population was reduced to 2.59 CFU/mL. The high inactivation of S. aureus was achieved under a duty cycle of 0.7:0.3 s with reduction of the population to 1.49 CFU/mL. The developed GA-ANN, which included 17 hidden neurons, could predict the S. aureus population with a coefficient of determination of 0.986. The overall agreement between ANFIS predictions and experimental data was also very good (R 2  = 0.979). Sensitivity analysis results showed that the amplitude level was the most sensitive factor for prediction of S. aureus.
      PubDate: 2018-06-01
      DOI: 10.1007/s13213-015-1067-4
      Issue No: Vol. 68, No. 6 (2018)
  • A novel isolate of Clostridium butyricum for efficient butyric acid
           production by xylose fermentation
    • Authors: Xin Wang; Jianzheng Li; Xue Chi; Yafei Zhang; Han Yan; Yu Jin; Juanjuan Qu
      Pages: 321 - 330
      Abstract: Bacterial fermentation of lignocellulose has been regarded as a sustainable approach to butyric acid production. However, the yield of butyric acid is hindered by the conversion efficiency of hydrolysate xylose. A mesophilic alkaline-tolerant strain designated as Clostridium butyricum B10 was isolated by xylose fermentation with acetic and butyric acids as the principal liquid products. To enhance butyric acid production, performance of the strain in batch fermentation was evaluated with various temperatures (20–47 °C), initial pH (5.0–10.0), and xylose concentration (6–20 g/L). The results showed that the optimal temperature, initial pH, and xylose concentration for butyric acid production were 37 °C, 9.0, and 8.00 g/L, respectively. Under the optimal condition, the yield and specific yield of butyric acid reached about 2.58 g/L and 0.36 g/g xylose, respectively, with 75.00% butyric acid in the total volatile fatty acids. As renewable energy, hydrogen was also collected from the xylose fermentation with a yield of about 73.86 mmol/L. The kinetics of growth and product formation indicated that the maximal cell growth rate (μ m ) and the specific butyric acid yield were 0.1466 h−1 and 3.6274 g/g cell (dry weight), respectively. The better performance in xylose fermentation showed C. butyricum B10 a potential application in efficient butyric acid production from lignocellulose.
      PubDate: 2018-06-01
      DOI: 10.1007/s13213-018-1340-4
      Issue No: Vol. 68, No. 6 (2018)
  • Transcriptomic profiling of maize ( Zea mays L.) seedlings in response to
           Pseudomonas putida stain FBKV2 inoculation under drought stress
    • Authors: Ali SkZ; Sandhya Vardharajula; Sai Shiva Krishna Prasad Vurukonda
      Pages: 331 - 349
      Abstract: Several mechanisms have been proposed for plant growth-promoting rhizobacteria (PGPR)-mediated drought stress tolerance in plants, but little is known about the molecular pathways involved in the drought tolerance promoted by PGPR. We, therefore, aim to study the differential gene response between Pseudomonas putida strain FBKV2 and maize interaction under drought stress using Illumina sequencing. RNA Seq libraries were generated from leaf tissue of maize seedlings with and without strain FBKV2 subjected to drought stress. The libraries were mapped with maize genome database for the identification of differentially expressed genes (DEGs). The expression studies confirmed the downregulation of ethylene biosynthesis (ET), abscisic acid (ABA) and auxin signaling, superoxide dismutase, catalase, and peroxidase in FBKV2-inoculated seedlings. On the other hand, genes involved in β-alanine and choline biosynthesis, heat shock proteins, and late embryogenesis abundant (LEA) proteins were upregulated, which could act as key elements in the drought tolerance conferred by P. putida strain FBKV2. Another remarkable expression was observed in genes encoding benzoxazinoid (BX) biosynthesis which act as the chemoattractant, which was further confirmed by gfp-labeled P. putida strain FBKV2 root colonization studies. Overall, these results indicate that secretion of BXs attracted P. putida strain FBKV2 resulted in root colonization and mediated drought tolerance by modulating metabolic, signaling, and stress-responsive genes.
      PubDate: 2018-06-01
      DOI: 10.1007/s13213-018-1341-3
      Issue No: Vol. 68, No. 6 (2018)
  • Optimization of date syrup as a novel medium for lovastatin production by
           Aspergillus terreus ATCC 20542 and analyzing assimilation kinetic of
    • Authors: Farshid Jaberi Ansari; Hassan Jalili; Marcin Bizukojc; Abdeltif Amrane
      Pages: 351 - 363
      Abstract: Lovastatin is a statin drug, which lowers cholesterol level in blood due to inhibition of (S)-3-hydroxy-3-methylglutaryl-CoA reductase. Date syrup is a rich medium for microbial growth and metabolite production. The main carbohydrates present in the date syrup are glucose and fructose. In this study, date syrup was used as a complex and bioresource medium for lovastatin production by Aspergillus terreus in the submerged cultivation. Optimization of the date syrup medium in order to achieve the highest titers of lovastatin and biomass was carried out. Four factors were studied by response surface methodology including concentration of date syrup carbohydrates, yeast extract concentration, pH, and rotation speed of the shaker. Optimal conditions for these factors found were as follows: concentration of date syrup carbohydrates, 64 g/l; yeast extract concentration, 15 g/l; pH, 6.5; and agitation speed, 150 rpm. It gave lovastatin concentration of 105.6 mg/l. Next, batch cultures in the optimal conditions were performed in a 2.5-l working volume bioreactor and led to the lovastatin titer of 241.1 mg/l during 12 days. Aspergillus terreus showed diauxic growth in the optimized medium with a shift from glucose to fructose assimilation during the run. Glucose and fructose assimilation kinetic parameters revealed that more lovastatin is produced during glucose assimilation, while more biomass was formed during fructose assimilation.
      PubDate: 2018-06-01
      DOI: 10.1007/s13213-018-1342-2
      Issue No: Vol. 68, No. 6 (2018)
  • Staphylococcus cohnii HFUTY-08: a novel acid urease-producing strain
    • Authors: Aona Zhang; Kun Liu; Jingjing Cao; Wanghui Yan; Yue Zheng; Qingmei Zeng
      Pages: 365 - 374
      Abstract: Urea in alcoholic beverages is a precursor of ethyl carbamate (EC), which is carcinogenic. At present, removal of urea by acid urease is considered to be the most effective method. In this study, a strain with higher acid urease production was screened and the enzyme activity was 1.12 U/mL. The strain was identified as Staphylococcus cohnii via a phylogenetic analysis of its 16S rDNA gene sequence, its morphological characteristics, and its physiological and biochemical properties, named as Staphylococcus cohnii HFUTY-08. Optimum culture conditions were determined through a single-factor test and an orthogonal test, with results as follows: glucose concentration 30 g/L, peptone concentration 15 g/L, initial pH 5, and an optimal inoculation amounts of 5%. Under these conditions, the activity of acid urease produced by strain Staphylococcus cohnii HFUTY-08 was 1.78 U/ml. Besides, the crude enzyme was purified to electrophoretic homogeneity by ion exchange chromatography and gel filtration chromatography. The molecular weight of the enzyme was estimated to be 295 kDa and the structural features of the enzyme were defined as (αβγ)3. Finally, the preliminary study on the removal of urea by acid urease in Chinese rice wine (CRW) showed that the enzyme could remove about 75% urea within 72 h at 37 °C, which effectively prevented EC production.
      PubDate: 2018-06-01
      DOI: 10.1007/s13213-018-1343-1
      Issue No: Vol. 68, No. 6 (2018)
  • Identification and application of a novel strong constitutive promoter in
           Corynebacterium glutamicum
    • Authors: Hongbo Wei; Yuechao Ma; Qixin Chen; Yi Cui; Lihong Du; Qian Ma; Yanjun Li; Xixian Xie; Ning Chen
      Pages: 375 - 382
      Abstract: The replacement of promoters with various strengths is an effective strategy to fine-tune gene expression. More available promoters with a broad range of transcription efficiency are needed in metabolic engineering. In this study, a putative protein coding gene CP_2454 was identified with a stable and high transcriptional level from an l-leucine-producing strain Corynebacterium glutamicum CP, which was absent in wild-type C. glutamicum ATCC 13032. The transcriptional level of CP_2454 was about 80.0% those of tuf and sod, and was 1.6 and 3.2 times those of ilvB and gapA, respectively. These promoters were cloned into C. glutamicum ATCC 13032 to control the expression of green fluorescent protein (GFP). While, the expressional level of GFP under the control of PCP_2454 was close to that of Ptuf and Psod, which was significantly higher than that of other tested promoters. The native promoter of ilvB controlling the expression of a feedback-resistant acetolactate synthase was replaced by PCP_2454, resulting in an increase of l-valine titer by 58.5%. A further 24.9% increase of l-valine titer was achieved after replacement of the promoter of ilvD encoding dihydroxyacid dehydratase with PCP_2454. Identification of the constitutive promoter PCP_2454 expands the promoter library of C. glutamicum and provides essential reference for tunable expression of target genes in C. glutamicum.
      PubDate: 2018-06-01
      DOI: 10.1007/s13213-018-1344-0
      Issue No: Vol. 68, No. 6 (2018)
  • Characterization of bacteriocins produced by strains of Pediococcus
           pentosaceus isolated from Minas cheese
    • Authors: Carolina Gutiérrez-Cortés; Héctor Suarez; Gustavo Buitrago; Luis Augusto Nero; Svetoslav Dimitrov Todorov
      Pages: 383 - 398
      Abstract: Interest in obtaining bacteriocin-producing strains of lactic acid bacteria (LAB) from different sources has been increasing in recent years due to their multiple applications in health and food industries. This study focused on the isolation and characterization of metabolically active populations of bacteriocinogenic LAB and the evaluation of their antimicrobial substances as well as of some nutritional requirements of them. One hundred and fifty colonies of LAB from artisanal cheeses produced in Minas Gerais state (Brazil) were isolated and screened for their antimicrobial activity. According to their activity against Listeria monocytogenes, ten strains were selected and subsequently identified using biochemical and molecular techniques including 16s rRNA amplification and sequencing as Enterococcus faecalis, Lactobacillus spp., and Pediococcus pentosaceus. Antimicrobial substances produced by four of the selected strains, P. pentosaceus 63, P. pentosaceus 145, P. pentosaceus 146, and P. pentosaceus 147, were biochemically characterized, and presented sensitivity to proteolytic enzymes (suggesting their proteinaceous nature) and to extreme pH. Antimicrobial activity showed stability after treatment with lipase, catalase, α-amylase, and chemicals. Growth kinetics of the P. pentosaceus selected showed maximal bacteriocin production at 37 °C during the end of the exponential growth phase (25,600 AU/mL) and stable production during 24 h of incubation. Dextrose, maltose, and a mixture of peptone, meat extract, and yeast extract increased bacteriocin production. This study demonstrated that dairy products provide a good alternative for obtaining LAB, with the ability to produce antimicrobial substances such as bacteriocins that have potential use as biopreservatives in food.
      PubDate: 2018-06-01
      DOI: 10.1007/s13213-018-1345-z
      Issue No: Vol. 68, No. 6 (2018)
  • Vertical profiles of microbial communities in perfluoroalkyl
           substance-contaminated soils
    • Authors: Yixiang Bao; Bingxin Li; Shuguang Xie; Jun Huang
      Pages: 399 - 408
      Abstract: Poly- and perfluoroalkyl compounds (PFASs) are ubiquitous in the environment, but their influences on microbial community remain poorly known. The present study investigated the depth-related changes of archaeal and bacterial communities in PFAS-contaminated soils. The abundance and structure of microbial community were characterized using quantitative PCR and high-throughput sequencing, respectively. Microbial abundance changed considerably with soil depth. The richness and diversity of both bacterial and archaeal communities increased with soil depth. At each depth, bacterial community was more abundant and had higher richness and diversity than archaeal community. The structure of either bacterial or archaeal community displayed distinct vertical variations. Moreover, a higher content of perfluorooctane sulfonate (PFOS) could have a negative impact on bacterial richness and diversity. The rise of soil organic carbon content could increase bacterial abundance but lower the richness and diversity of both bacterial and archaeal communities. In addition, Proteobacteria, Actinobacteria, Chloroflexi, Cyanobacteria, and Acidobacteria were the major bacterial groups, while Thaumarchaeota, Euryarchaeota, and unclassified Archaea dominated in soil archaeal communities. PFASs could influence soil microbial community.
      PubDate: 2018-06-01
      DOI: 10.1007/s13213-018-1346-y
      Issue No: Vol. 68, No. 6 (2018)
  • Deciphering the mode of action, structural and biochemical analysis of
           heparinase II/III ( Ps PL12a) a new member of family 12 polysaccharide
           lyase from Pseudopedobacter saltans
    • Authors: Karthika Balasubramaniam; Kedar Sharma; Aruna Rani; Vikky Rajulapati; Arun Goyal
      Pages: 409 - 418
      Abstract: Heparinases are widely used for production of clinically and therapeutically important bioactive oligosaccharides and in analyzing the polydisperse, heterogeneous, and complex structures of heparin/heparan sulfate. In the present study, the gene (1911 bp) encoding heparinase II/III of family 12 polysaccharide lyase (PsPL12a) from Pseudopedobacter saltans was cloned, expressed, and biochemically and functionally characterized. The purified enzyme PsPL12a of molecular size approximately 76 kDa exhibited maximum activity in the temperature range 45–50 °C and at pH 6.0. PsPL12a gave maximum activity at 1% (w/v) heparin under optimum conditions. The kinetic parameters, K m and Vmax, for PsPL12a were 4.6 ± 0.5 mg/ml and 70 ± 2 U/mg, respectively. Ten millimolars of each Mg2+ and Mn2+ ions enhanced PsPL12a activity by 80%, whereas Ni2+ inhibited by 75% and Co2+ by 10%, and EDTA completely inactivated the enzyme. Protein melting curve of PsPL12a gave a single peak at 55 °C and 10 mM Mg2+ ions and shifted the peak to 60 °C. The secondary structure analysis of PsPL12a by CD showed 65.12% α-helix, 11.84% β-strand, and 23.04% random coil. The degradation products of heparin by PsPL12a analyzed by ESI-MS spectra displayed peaks corresponding to heparin di-, tetra-, penta-, and hexa-saccharides revealing the endolytic mode of enzyme action. Heparinase II/III (PsPL12a) from P. saltans can be used for production of low molecular weight heparin oligosaccharides for their utilization as anticoagulants. This is the first report on heparinase cloned from P. saltans.
      PubDate: 2018-06-01
      DOI: 10.1007/s13213-018-1347-x
      Issue No: Vol. 68, No. 6 (2018)
  • Characterization of As(III) oxidizing Achromobacter sp. strain N2: effects
           on arsenic toxicity and translocation in rice
    • Authors: Anna Corsini; Milena Colombo; Claudio Gardana; Sarah Zecchin; Paolo Simonetti; Lucia Cavalca
      Pages: 295 - 304
      Abstract: Achromobacter sp. strain N2 was isolated from a pyrite-cinder-contaminated soil and presented plant growth promoting traits (ACC deaminase activity, production of indole-3-acetic and jasmonic acids, siderophores secretion, and phosphate solubilization) and arsenic transformation abilities. Achromobacter sp. strain N2 was resistant to different metals and metalloids, including arsenate (100 mM) and arsenite (5 mM). The strain was resistant to ionic stressors (i.e., arsenate and NaCl), whereas bacterial growth was impaired by osmotic stress. Strain N2 was able to oxidize 1.0 mmol L−1 of arsenite to arsenate in 72 h. This evidence was supported by the retrieval of an arsenite oxidase AioA gene highly homologous to arsenite oxidases of Achromobacter and Alcaligenes species. Rice seeds of Oryza sativa (var. Loto) were bio-primed with ACCD-induced and non-induced cells in order to evaluate the effect of inoculation on rice seedlings growth and arsenic uptake. The bacterization with ACCD-induced cells significantly improved seed germination and seedling heights if compared with the seeds inoculated with non-induced cells and non-primed seeds. Enhanced arsenic uptake was evidenced in the presence of ACCD-induced cells, suggesting a role of ACCD activity on the mitigation of the toxicity of arsenic accumulated by the plant. This kind of responses should be taken into account when proposing PGP strains for improving plant growth in arsenic-rich soils.
      PubDate: 2018-05-01
      DOI: 10.1007/s13213-018-1338-y
      Issue No: Vol. 68, No. 5 (2018)
  • Isolation and characterization of a new fructophilic Lactobacillus
           plantarum FPL strain from honeydew
    • Authors: Klaudia Gustaw; Magdalena Michalak; Magdalena Polak-Berecka; Adam Waśko
      Abstract: In the present study, a Lactobacillus plantarum FPL strain exhibiting fructophilic behavior has been isolated for the first time from honeydew. It is a probably syntrophic bacterium inhabiting the gastrointestinal tract of Coccus hesperidum L. and taking part in sugar metabolism. The promising growth characteristics and biochemical properties of Lb. plantarum FPL indicate that this may be a facultatively fructophilic species, whose properties are not associated with the loss of the alcohol/acetaldehyde dehydrogenase gene. The article attempts to classify the peculiar behavior of this strain by means of tests that are characteristic for FLAB as well as through a classic identification approach. In this study, we used a reference strain Lb. plantarum NRRL B-4496, which showed no fructophilic properties. With the FLAB group, the new strain shares the habit, such as a fructose-rich environment, the preference of this sugar for growth, and similar growth curves. However, it exceeds FLAB in terms of osmotolerance to high sugar content. The fructophilic Lb. plantarum FPL strain can proliferate and grow on a medium wherein the sugar concentration is 45 and 50% (w/v). Our findings indicate that honeydew can be a promising source of new fructophilic lactic acid bacteria.
      PubDate: 2018-06-01
      DOI: 10.1007/s13213-018-1350-2
  • Screening and molecular identification of potential probiotic lactic acid
           bacteria in effluents generated during ogi production
    • Authors: Oladipupo Odunayo Olatunde; Adewale Olusegun Obadina; Adebukunola Mobolaji Omemu; Olusola Bandele Oyewole; Adetola Olugbile; Oladapo Oluwaseye Olukomaiya
      Abstract: Screening and molecular identification of probiotic lactic acid bacteria (LAB) in effluents generated during the production of ogi, a fermented cereal (maize, millet, and sorghum) were done. LAB were isolated from effluents generated during the first and second fermentation stages in ogi production. Bacterial strains isolated were identified microscopically and phenotypically using standard methods. Probiotic potential properties of the isolated LAB were investigated in terms of their resistance to pH 1.5 and 0.3% bile salt concentration for 4 h. The potential LAB isolates ability to inhibit the growth of pathogenic organisms (Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium) was evaluated in vitro. The pH and LAB count in the effluents ranged from 3.31 to 4.49 and 3.67 to 4.72 log cfu/ml, respectively. A total of 88 LAB isolates were obtained from the effluents and only 10 LAB isolates remained viable at pH 1.5 and 0.3% bile salt. The zones of inhibition of the LAB isolates with probiotic potential ranged from 7.00 to 24.70 mm against test organsisms. Probiotic potential LAB isolates were molecularly identified as Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus reuteri, Enterococcus faecium, Pediococcus acidilactici, Pediococcus pentosaceus, Enterococcus faecalis, and Lactobacillus brevis. Survival and proliferation of LAB isolates at low pH, 0.3% bile salt condition, and their inhibition against some test pathogens showed that these LAB isolates could be a potential probiotics for research and commercial purposes.
      PubDate: 2018-05-25
      DOI: 10.1007/s13213-018-1348-9
  • Industrial wastewaters harbor a unique diversity of bacterial communities
           revealed by high-throughput amplicon analysis
    • Authors: Ramganesh Selvarajan; Timothy Sibanda; Siddarthan Venkatachalam; Ilunga Kamika; Willem A. J. Nel
      Abstract: Industrial wastewater effluents present a major source of water pollution, and can potentially alter the microbial ecological landscape. While there are numerous reports on the microbial quality of domestic municipal effluents and their perceived environmental effects, there are limited reports devoted to the study of bacterial diversity of effluents from individual industries before they are mixed up with other sources. This study analyzed both the physicochemical parameters and bacterial community structures of different industrial wastewaters using Illumina high-throughput sequencing platform. Industrial wastewater with temperature ranging from 18.9 to 21.5 °C, and total dissolved solid (TDS) levels at up to 4611 mg/L, appeared to be predominated by Proteobacteria (44.44–75.86%) with the exception of the Capegate sample where Actinobacteria (39.66%) were the highest. Sulfur levels were significantly higher (p < 0.05) in Dixon wastewater constituting higher populations of sulfur reducing bacteria (SRB) compared to the other sites. Diversity index (Shannon-H index) and richness estimator (Chao1 index) ranged from 974 (Capegate) to 4552 (Dixon) and 6.04 (Dixon) to 4.15 (CWI), respectively. Multivariate analysis results highlighted that the bacterial communities were strongly shaped by physicochemical variables. The top 10 operational taxonomic units (OTUs) of each industrial sample had the potential to play important roles in the bioremediation and biodegradation of pollutants. Dominant OTUs belonging to the phyla Planctomyces from the Chemreem sample could not be classified to any genera and are likely to represent novel species.
      PubDate: 2018-05-23
      DOI: 10.1007/s13213-018-1349-8
  • Screening and identification of an Enterobacter ludwigii strain expressing
           an active β -xylosidase
    • Authors: Jiashi Zhang; Tangbing Cui; Xueqing Li
      Abstract: Researchers have expressed increasing interest in the xylanolytic enzymes used in hemicellulose hydrolysis that convert wood and agricultural residues to second-generation biofuels. In our study, 32 isolates showed clear hydrolysis zones on agar plates containing xylan after Congo red staining. Among these isolates, strain LY-62 exhibited the highest β-xylosidase activity (1.29 ± 0.05 U/mL). According to the phylogenetic analysis of the 16S rDNA, strain LY-62 belongs to the Enterobacter genus. Using a combination of electron microscopy, Gram-staining, and conventional physiological and biochemical examinations, the strain LY-62 was identified as Enterobacter ludwigii. The β-xylosidase gene from Enterobacter ludwigii LY-62 was cloned, and the full-length protein was expressed in Escherichia coli as an N-terminal or C-terminal His-tagged fusions protein. Optimal β-xylosidase activity was achieved at pH 7.0 and 40 °C. The Michaelis constant KM values for His-Xyl62 and Xyl62-His were 1.55 and 2.8 mmol/L, respectively. The kcat values for His-Xyl62 and Xyl62-His were 8.51 and 6.94 s−1, respectively. The catalytic efficiencies of His-Xyl62 and Xyl62-His were 5.49 and 2.48 s−1 × mM−1, respectively. Thus, Xyl62 is a functional β-xylosidase, and our study represents the first report of a β-xylosidase from Enterobacter ludwigii.
      PubDate: 2018-04-12
      DOI: 10.1007/s13213-018-1334-2
  • Effect of inorganic salt stress on the thermotolerance and ethanol
           production at high temperature of Pichia kudriavzevii
    • Authors: Chunsheng Li; Laihao Li; Xianqing Yang; Yanyan Wu; Yongqiang Zhao; Yueqi Wang
      Abstract: Application of cross-protection is expected to improve the thermotolerance of yeasts to enhance their ethanol production at high temperature. In this study, the effects of eight kinds of inorganic salts on the thermotolerance and ethanol production at high temperature in Pichia kudriavzevii were investigated. P. kudriavzevii showed strong thermotolerance and the ability to produce ethanol at high temperature, and higher ethanol production of P. kudriavzevii was observed at high temperature (37–42 °C) compared with that at 30 °C. Inorganic salt stresses induced obvious cross-protection of thermotolerance in P. kudriavzevii. The presence of 0.1 mol/L KNO3 or Na2SO4 or 0.2 mol/L NaCl, KCl, NaNO3, K2SO4 or MgCl2 increased the yeast biomass in YEPD medium at 44 °C to 2.72–3.46 g/L, obviously higher than that in the absence of salt stress (2.17 g/L). The addition of NaCl, KCl, NaNO3, KNO3, Na2SO4, K2SO4, CaCl2 and MgCl2 significantly increased the ethanol production of P. kudriavzevii in YEPD fermentation medium at 44 °C by 37–58%. KCl and MgCl2 exhibited the best performance on improving the thermotolerance and ethanol production, respectively, of P. kudriavzevii. A highly significant correlation (P < 0.01) was obtained among ethanol production, biomass and glucose consumption, suggesting the important role of thermotolerance and glucose consumption in enhanced ethanol production. The combination of NaCl, KCl and MgCl2 had a synergistic effect on the improvement of thermotolerance and ethanol production at high temperature in P. kudriavzevii. This study provides some important clues for improving ethanol production of thermotolerant yeasts at high temperature.
      PubDate: 2018-04-10
      DOI: 10.1007/s13213-018-1339-x
  • The response of LuxS/AI-2 quorum sensing in Lactobacillus fermentum 2-1 to
           changes in environmental growth conditions
    • Authors: Yue Gu; Bo Li; Jianjun Tian; Rong Wu; Yinfeng He
      Abstract: Quorum sensing (QS) is a communication mechanism based on the production of autoinducers in order to regulate cooperative behavior and physiological traits among bacteria. Autoinducer-2 (AI-2) is mediated by luxS and pfs genes and is used for both intra- and interspecies communication. The effects of environmental stresses, such as pH, temperature, osmotic, and nutrient stresses, were investigated on the LuxS/AI-2 QS system in Lactobacillus fermentum 2-1. The AI-2 activity in L. fermentum 2-1 was induced by stress responses to acid shock, high temperature, and nutrient inadequacy while a decrease in activity was correlated with high pH, low temperature, and osmotic stress. Maximum transcription of luxS and pfs was achieved after L. fermentum 2-1 was exposed to acid shock, low temperature, high concentration of NaCl, and diluted MRS broth. The results indicate that various stresses affected the LuxS/AI-2 QS system, which may be involved in the resistance process of the L. fermentum 2-1.
      PubDate: 2018-03-30
      DOI: 10.1007/s13213-018-1337-z
  • Bacterial community structure associated with the rhizosphere soils and
           roots of Stellera chamaejasme L. along a Tibetan elevation gradient
    • Authors: Hui Jin; Xiaoyan Yang; Rentao Liu; Zhiqiang Yan; Xudong Li; Xiuzhuang Li; Anxiang Su; Yuhui Zhao; Bo Qin
      Abstract: The effect of altitude on the composition and diversity of microbial communities have attracted highly attention recently but is still poorly understood. We used 16S rRNA gene clone library analyses to characterize the bacterial communities from the rhizosphere and roots of Stellera chamaejasme in the Tibetan Plateau. Our results revealed that Actinobacteria and Proteobacteria were dominant bacteria in this medicinal plant in the rhizosphere and root communities. The Shannon diversity index showed that the bacterial diversity of rhizosphere follows a small saddle pattern, while the roots possesses of a hump-backed trend. Significant differences in the composition of bacterial communities between rhizosphere and roots were detected based on multiple comparisons analysis. The community of Actinobacteria was found to be significantly negative correlated with soil available P (p < 0.01), while the phylum of Proteobacteria showed a positive relationship with available P (p < 0.05). Moreover, redundancy analysis indicated that soil phosphorus, pH, latitude, elevation and potassium positively correlated with bacterial communities associated with rhizosphere soils. Taken together, we provide evidence that bacterial communities associated with S. chamaejasme exhibited some certain elevational pattern, and bacterial communities of rhizosphere soil were regulated by environmental characteristics along elevational gradients in this alpine ecosystem.
      PubDate: 2018-03-30
      DOI: 10.1007/s13213-018-1336-0
  • Diversity and symbiotic divergence of endophytic and non-endophytic
           rhizobia of Medicago sativa
    • Authors: Wenjuan Kang; Shangli Shi; Lin Xu
      Abstract: Knowledge of rhizobium diversity is helping to enable the utilization of rhizobial resources. To analyze the phenotypic and genetic diversity and the symbiotic divergence of rhizobia of Medicago sativa, 30 endophytic and non-endophytic isolates were collected from different parts of five alfalfa varieties in three geographic locations in Gansu, China. Numerical analyses based on 72 phenotypic properties and restriction fragment length polymorphism (RFLP) fingerprinting indicated the abundant phenotypic and genetic diversity of the tested strains. According to the phylogenetic analysis of 16S RNA, atpD, glnII, and recA gene sequences, Rhizobium and Ensifer were further classified into four different genotypes: Rhizobium radiobacter, Rhizobium sp., Rhizobium rosettiformans, and Ensifer meliloti. The differences in architecture and functioning of the rhizobial genomes and, to a lesser extent, environment diversification helped explain the diversity of tested strains. The tested strains exhibited similar symbiotic feature when inoculated onto M. sativa cvs. Gannong Nos. 3 and 9 and Qingshui plants for the clustering feature of their parameter values. An obvious symbiotic divergence of rhizobial strains was observed in M. sativa cvs. Longzhong and WL168HQ plants because of the scattered parameter values. Their symbiotic divergence differed according to alfalfa varieties, which indicated that the sensitivity of different alfalfa varieties to rhizobial strains may differ. Most of the tested strains exhibited plant growth-promoting traits including phosphate solubilization and production of indole-3-acetic acid (IAA) when colonizing plant tissues and soil.
      PubDate: 2018-03-26
      DOI: 10.1007/s13213-018-1333-3
  • Prospecting the characteristics and significance of the phyllosphere
    • Authors: Shobit Thapa; Radha Prasanna
      Abstract: Phyllosphere represents the largest global interface of the aerial parts of the plant, comprising mainly stem and leaves, which is inhabited by various groups of microorganisms. Analyses of the spatial abundance of microflora, diversity, and distribution of microbial communities and the influence of abiotic and biotic factors have revealed that this niche is unique. This reflects the impact of both evolutionary and ecological factors, leading to sorting of microbial species, delineation of keystone species or microbial hubs, mediated by inter-kingdom connectivity and networking. Production of hormones, pigments, volatiles, extracellular polysaccharides (EPS), cross-kingdom signals, and quorum sensing are characteristic facets, which promote proliferation and survival in the harsh and inhospitable habitat of the phyllosphere, exposed to radiation and environmental extremes. The use of both traditional morphology-culturing-based taxonomy and modern tools of metagenomics, proteomics, and transcriptomics has illustrated that the diversity among bacterial members is mainly restricted to Actinobacteria, Proteobacteria, Firmicutes, Bacteroidetes, and less frequently, Cyanobacteria; oomycetous communities are common inhabitants, besides fungi. Despite scanty published work on foliar disease-related aspects, the phyllosphere can provide a model microenvironment, in which the interactions between the pathogen and biocontrol agent can be visualized and modulated. The major aims of the present review are as follows: (i) to elucidate the mechanisms of microbial colonization and decipher the nature of spatial and temporal changes in the abundance and diversity in this niche; (ii) to illustrate the significance of the different taxonomic groups; and (iii) outline future strategies for research on the phyllosphere microbiome.
      PubDate: 2018-03-19
      DOI: 10.1007/s13213-018-1331-5
  • The significance of the diversity and composition of the cecal microbiota
           of the Tibetan swine
    • Authors: Weiping Yang; Haiyun Xin; Fangjun Cao; Jinxing Hou; Li Ma; Lijuan Bao; Fangyuan Wang; Zhantao Yu; Binyun Cao
      Abstract: The Tibetan swine (TIS) is a non-ruminant herbivore with high disease resistance. Also, it has the ability to digest plants with high fiber content. However, it is not known whether any relationship exist between these characteristics of the TIS and its cecal microbiota. Thus, this study aims to investigate the cecal microbiota of the adult TIS using high-throughput sequencing techniques in order to explore possible relationships between these unique characteristics of the TIS (high disease resistance and ability to digest high fiber plants) and its cecal microbiota. PIC pigs (lean type) were chosen as controls. The results show that 75,069 valid sequences of the 16S rRNA gene at V4-V5 region were obtained in the cecal content of TIS. They were composed of 15 phyla, 70 genera and divided into 660 Operational Taxonomic Units (OTUs). Bacteroidetes and Firmicutes were the predominant phyla in both breeds, but TIS had more Bacteroidetes than Firmicutes. Also, 42.4% of the cecal bacteria were found to be unclassified and uncultured. Many cellulolytic bacteria were also found in the two breeds. TIS (88.10%) had much higher abundance in the core bacterial communities than PIC pigs (81.29%), and the proportion of Bacteroides and Spirochaetes that can effectively degrade cellulose were 6.01 and 6.40% higher than PIC pigs, respectively, while Proteobacteria that are closely related to gastrointestinal diseases were 1.61% lower than PIC pigs. Thus, the disease resistance of the TIS and its ability to digest plants with high fiber content may be related to high abundance of core bacterial communities as well as the large number of unknown and unclassified bacteria.
      PubDate: 2018-03-09
      DOI: 10.1007/s13213-018-1329-z
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