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Showing 1 - 200 of 3562 Journals sorted alphabetically
16 de Abril     Open Access   (Followers: 1)
3D Printing in Medicine     Open Access   (Followers: 4)
4 open     Open Access  
AADE in Practice     Hybrid Journal   (Followers: 6)
ABCS Health Sciences     Open Access   (Followers: 7)
Abia State University Medical Students' Association Journal     Full-text available via subscription   (Followers: 2)
AboutOpen     Open Access   (Followers: 1)
ACIMED     Open Access   (Followers: 1)
ACS Medicinal Chemistry Letters     Hybrid Journal   (Followers: 48)
Acta Bio Medica     Full-text available via subscription   (Followers: 2)
Acta Bioethica     Open Access  
Acta Bioquimica Clinica Latinoamericana     Open Access   (Followers: 1)
Acta Científica Estudiantil     Open Access  
Acta Facultatis Medicae Naissensis     Open Access   (Followers: 1)
Acta Herediana     Open Access  
Acta Informatica Medica     Open Access  
Acta Medica (Hradec Králové)     Open Access  
Acta Medica Bulgarica     Open Access  
Acta Medica Colombiana     Open Access   (Followers: 1)
Acta Médica Costarricense     Open Access   (Followers: 2)
Acta Medica Indonesiana     Open Access  
Acta Medica International     Open Access  
Acta medica Lituanica     Open Access  
Acta Medica Marisiensis     Open Access  
Acta Medica Martiniana     Open Access  
Acta Medica Nagasakiensia     Open Access   (Followers: 1)
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Acta Médica Portuguesa     Open Access  
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Acta Scientiarum. Health Sciences     Open Access   (Followers: 2)
Acupuncture & Electro-Therapeutics Research     Full-text available via subscription   (Followers: 6)
Acupuncture and Natural Medicine     Open Access  
Addiction Science & Clinical Practice     Open Access   (Followers: 8)
Addictive Behaviors Reports     Open Access   (Followers: 9)
Adıyaman Üniversitesi Sağlık Bilimleri Dergisi / Health Sciences Journal of Adıyaman University     Open Access   (Followers: 1)
Adnan Menderes Üniversitesi Sağlık Bilimleri Fakültesi Dergisi     Open Access  
Advanced Biomedical Research     Open Access  
Advanced Health Care Technologies     Open Access   (Followers: 8)
Advanced Science, Engineering and Medicine     Partially Free   (Followers: 8)
Advanced Therapeutics     Hybrid Journal   (Followers: 1)
Advances in Bioscience and Clinical Medicine     Open Access   (Followers: 7)
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Advances in Clinical Chemistry     Full-text available via subscription   (Followers: 26)
Advances in Life Course Research     Hybrid Journal   (Followers: 9)
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Advances in Medical Education and Practice     Open Access   (Followers: 32)
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Advances in Medical Sciences     Hybrid Journal   (Followers: 9)
Advances in Medicinal Chemistry     Full-text available via subscription   (Followers: 6)
Advances in Medicine     Open Access   (Followers: 3)
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Advances in Molecular Oncology     Open Access   (Followers: 2)
Advances in Molecular Toxicology     Full-text available via subscription   (Followers: 7)
Advances in Parkinson's Disease     Open Access  
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Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 20)
Advances in Regenerative Medicine     Open Access   (Followers: 3)
Advances in Skeletal Muscle Function Assessment     Open Access  
Advances in Therapy     Hybrid Journal   (Followers: 5)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 15)
Advances in Virus Research     Full-text available via subscription   (Followers: 6)
Advances in Wound Care     Hybrid Journal   (Followers: 12)
Aerospace Medicine and Human Performance     Full-text available via subscription   (Followers: 13)
African Health Sciences     Open Access   (Followers: 3)
African Journal of Biomedical Research     Open Access   (Followers: 1)
African Journal of Clinical and Experimental Microbiology     Open Access   (Followers: 4)
African Journal of Laboratory Medicine     Open Access   (Followers: 2)
African Journal of Medical and Health Sciences     Open Access   (Followers: 3)
African Journal of Thoracic and Critical Care Medicine     Open Access  
African Journal of Trauma     Open Access   (Followers: 1)
Afrimedic Journal     Open Access   (Followers: 2)
Aggiornamenti CIO     Hybrid Journal   (Followers: 1)
AIDS Research and Human Retroviruses     Hybrid Journal   (Followers: 9)
AJOB Empirical Bioethics     Hybrid Journal   (Followers: 3)
AJSP: Reviews & Reports     Hybrid Journal  
Aktuelle Ernährungsmedizin     Hybrid Journal   (Followers: 4)
Al-Azhar Assiut Medical Journal     Open Access   (Followers: 2)
ALERTA : Revista Científica del Instituto Nacional de Salud     Open Access  
Alexandria Journal of Medicine     Open Access   (Followers: 1)
Allgemeine Homöopathische Zeitung     Hybrid Journal   (Followers: 3)
Alpha Omegan     Full-text available via subscription  
ALTEX : Alternatives to Animal Experimentation     Open Access   (Followers: 2)
Althea Medical Journal     Open Access   (Followers: 2)
American Journal of Biomedical Engineering     Open Access   (Followers: 15)
American Journal of Biomedical Research     Open Access   (Followers: 2)
American Journal of Biomedicine     Full-text available via subscription   (Followers: 7)
American Journal of Chinese Medicine, The     Hybrid Journal   (Followers: 4)
American Journal of Clinical Medicine Research     Open Access   (Followers: 8)
American Journal of Family Therapy     Hybrid Journal   (Followers: 10)
American Journal of Law & Medicine     Full-text available via subscription   (Followers: 11)
American Journal of Lifestyle Medicine     Hybrid Journal   (Followers: 5)
American Journal of Managed Care     Full-text available via subscription   (Followers: 12)
American Journal of Medical Case Reports     Open Access   (Followers: 1)
American Journal of Medical Sciences and Medicine     Open Access   (Followers: 4)
American Journal of Medicine     Hybrid Journal   (Followers: 50)
American Journal of Medicine and Medical Sciences     Open Access   (Followers: 1)
American Journal of Medicine Studies     Open Access   (Followers: 2)
American Journal of Medicine Supplements     Full-text available via subscription   (Followers: 3)
American Journal of the Medical Sciences     Hybrid Journal   (Followers: 12)
American Journal on Addictions     Hybrid Journal   (Followers: 10)
American medical news     Free   (Followers: 3)
American Medical Writers Association Journal     Full-text available via subscription   (Followers: 5)
Amyloid: The Journal of Protein Folding Disorders     Hybrid Journal   (Followers: 5)
Anales de la Facultad de Medicina     Open Access  
Anales de la Facultad de Medicina, Universidad de la República, Uruguay     Open Access  
Anales del Sistema Sanitario de Navarra     Open Access   (Followers: 1)
Analgesia & Resuscitation : Current Research     Hybrid Journal   (Followers: 6)
Anatolian Clinic the Journal of Medical Sciences     Open Access  
Anatomica Medical Journal     Open Access  
Anatomical Science International     Hybrid Journal   (Followers: 3)
Anatomical Sciences Education     Hybrid Journal   (Followers: 1)
Anatomy     Open Access   (Followers: 2)
Anatomy Research International     Open Access   (Followers: 3)
Angewandte Schmerztherapie und Palliativmedizin     Hybrid Journal  
Angiogenesis     Hybrid Journal   (Followers: 3)
Ankara Medical Journal     Open Access   (Followers: 2)
Ankara Üniversitesi Tıp Fakültesi Mecmuası     Open Access  
Annales de Pathologie     Full-text available via subscription  
Annales des Sciences de la Santé     Open Access  
Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale     Full-text available via subscription   (Followers: 3)
Annals of African Medicine     Open Access   (Followers: 2)
Annals of Anatomy - Anatomischer Anzeiger     Hybrid Journal   (Followers: 3)
Annals of Bioanthropology     Open Access   (Followers: 5)
Annals of Biomedical Engineering     Hybrid Journal   (Followers: 19)
Annals of Biomedical Sciences     Full-text available via subscription   (Followers: 4)
Annals of Clinical Hypertension     Open Access  
Annals of Clinical Microbiology and Antimicrobials     Open Access   (Followers: 14)
Annals of Family Medicine     Open Access   (Followers: 14)
Annals of Health Research     Open Access   (Followers: 1)
Annals of Ibadan Postgraduate Medicine     Open Access  
Annals of Medical and Health Sciences Research     Open Access   (Followers: 7)
Annals of Medicine     Hybrid Journal   (Followers: 12)
Annals of Medicine and Surgery     Open Access   (Followers: 7)
Annals of Microbiology     Hybrid Journal   (Followers: 13)
Annals of Nigerian Medicine     Open Access   (Followers: 1)
Annals of Rehabilitation Medicine     Open Access  
Annals of Saudi Medicine     Open Access  
Annals of the New York Academy of Sciences     Hybrid Journal   (Followers: 5)
Annals of The Royal College of Surgeons of England     Full-text available via subscription   (Followers: 3)
Annual Reports in Medicinal Chemistry     Full-text available via subscription   (Followers: 7)
Annual Reports on NMR Spectroscopy     Full-text available via subscription   (Followers: 5)
Annual Review of Medicine     Full-text available via subscription   (Followers: 17)
Anthropological Review     Open Access   (Followers: 24)
Anthropologie et santé     Open Access   (Followers: 5)
Antibiotics     Open Access   (Followers: 9)
Antibodies     Open Access   (Followers: 2)
Antibody Technology Journal     Open Access   (Followers: 1)
Antibody Therapeutics     Open Access  
Anuradhapura Medical Journal     Open Access  
Anwer Khan Modern Medical College Journal     Open Access   (Followers: 2)
Apmis     Hybrid Journal   (Followers: 1)
Apparence(s)     Open Access   (Followers: 1)
Applied Clinical Informatics     Hybrid Journal   (Followers: 4)
Applied Clinical Research, Clinical Trials and Regulatory Affairs     Hybrid Journal   (Followers: 2)
Applied Medical Informatics     Open Access   (Followers: 13)
Arab Journal of Nephrology and Transplantation     Open Access   (Followers: 1)
Archive of Clinical Medicine     Open Access   (Followers: 1)
Archive of Community Health     Open Access   (Followers: 1)
Archives Medical Review Journal / Arşiv Kaynak Tarama Dergisi     Open Access  
Archives of Asthma, Allergy and Immunology     Open Access  
Archives of Medical and Biomedical Research     Open Access   (Followers: 3)
Archives of Medical Laboratory Sciences     Open Access   (Followers: 1)
Archives of Medicine and Health Sciences     Open Access   (Followers: 4)
Archives of Medicine and Surgery     Open Access  
Archives of Trauma Research     Open Access   (Followers: 3)
Archivos de Medicina (Manizales)     Open Access  
ArgoSpine News & Journal     Hybrid Journal  
Arquivos Brasileiros de Oftalmologia     Open Access   (Followers: 1)
Arquivos de Ciências da Saúde     Open Access  
Arquivos de Medicina     Open Access  
Ars Medica : Revista de Ciencias Médicas     Open Access  
ARS Medica Tomitana     Open Access   (Followers: 1)
Art Therapy: Journal of the American Art Therapy Association     Hybrid Journal   (Followers: 17)
Arterial Hypertension     Open Access   (Followers: 1)
Artificial Intelligence in Medicine     Hybrid Journal   (Followers: 18)
Artificial Organs     Hybrid Journal   (Followers: 1)
ASHA Leader     Open Access  
Asia Pacific Family Medicine     Open Access   (Followers: 1)
Asia Pacific Journal of Clinical Nutrition     Full-text available via subscription   (Followers: 12)
Asia Pacific Journal of Clinical Trials : Nervous System Diseases     Open Access  
Asian Bioethics Review     Full-text available via subscription   (Followers: 3)
Asian Biomedicine     Open Access   (Followers: 2)
Asian Journal of Cell Biology     Open Access   (Followers: 5)
Asian Journal of Health     Open Access   (Followers: 3)
Asian Journal of Medical and Biological Research     Open Access   (Followers: 4)
Asian Journal of Medical and Pharmaceutical Researches     Open Access   (Followers: 2)
Asian Journal of Medical Sciences     Open Access   (Followers: 2)
Asian Journal of Medicine and Health     Open Access  
Asian Journal of Research in Medical and Pharmaceutical Sciences     Open Access  
Asian Journal of Scientific Research     Open Access   (Followers: 3)
Asian Journal of Transfusion Science     Open Access   (Followers: 1)
Asian Medicine     Hybrid Journal   (Followers: 5)
Asian Pacific Journal of Cancer Prevention     Open Access  
ASPIRATOR : Journal of Vector-borne Disease Studies     Open Access  
Astrocyte     Open Access  
Atención Familiar     Open Access  
Atención Primaria     Open Access   (Followers: 1)
Atti della Accademia Peloritana dei Pericolanti - Classe di Scienze Medico-Biologiche     Open Access  

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Similar Journals
Journal Cover
Advances in Medical Sciences
Journal Prestige (SJR): 0.694
Citation Impact (citeScore): 2
Number of Followers: 9  
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1896-1126 - ISSN (Online) 1898-4002
Published by Elsevier Homepage  [3168 journals]
  • Comparison of the QuantiFERON-TB Gold Plus and QuantiFERON-TB Gold In-Tube
           interferon-γ release assays: A systematic review and meta-analysis
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Babak Pourakbari, Setareh Mamishi, Sepideh Benvari, Shima MahmoudiAbstractPurposeQuantiFERON-TB Gold Plus (QFT-Plus) is a new generation of QuantiFERON assay that differs from QuantiFERON-TB Gold In-Tube test (QFT-GIT). The aim of this study was to compare the performance of the new FDA-approved QFT-Plus interferon (IFN)-γ release assays (IGRAs) with the QFT-GIT version of this assay.Material and methodsWe searched all studies published in English in electronic databases, including PubMed, Scopus, and Web of Science.ResultsThe positive proportion of positive results by QFT-Plus was higher than QFT-GIT in cured tuberculosis (TB) cases (82% vs. 73%). The two tests showed a substantial agreement and the majority of the latent tuberculosis infection (LTBI) subjects responded concomitantly to both QFT-Plus and QFT-GIT. However, QFT-Plus showed a stronger association with surrogate measures of TB suspects than QFT-GIT. The QFT-Plus test demonstrated a higher sensitivity than QFT-GIT in the older adults. The sensitivity, specificity, LR+, LR- and DOR overall were 94% (95% CI 89–97), 96% (95% CI 94–98), 24.4 (95% CI 15–39), 0.05 (95% CI 0.03–0.11) and 414 (95% CI 251–685), respectively. The area under summary ROC curve was 0.99 (95% CI 0.97–0.99).ConclusionQFT-Plus performs equivalently to the QFT-GIT for detection of patients at risk for LTBI; however, QFT-Plus test had higher sensitivity than the QFT-GIT test, with similar specificity among the older participants. Higher IFN-γ release in TB2 compared to TB1 might be due to recent LTBI.
  • Expression of several long noncoding RNAs in peripheral blood mononuclear
           cells of patients with systemic lupus erythematosus
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Jie-Bing Wang, Jun Li, Tian-Ping Zhang, Tian-Tian Lv, Lian-Ju Li, Jun Wu, Rui-Xue Leng, Yin-Guang Fan, Hai-Feng Pan, Dong-Qing YeAbstractPurposeAccumulating evidence has linked long noncoding RNAs (lncRNAs) to autoimmune and inflammatory disorders. This study aimed to detect the expression levels of five lncRNAs (lnc0640, lnc3643, lnc5150, lnc7514 and lncagf) in peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SLE), as well as their correlation with clinical and laboratory features.Materials/methodsWe recruited 76 patients with SLE and 71 normal controls into the present study, and obtained PBMCs from the blood samples of all study subjects. Expression levels of lncRNAs were determined by quantitative real-time reverse transcription polymerase chain reaction and their associations with clinical and laboratory characteristics were analyzed.ResultsLnc5150 expression levels were statistically significantly decreased (Z=−6.016, P 
  • Cell adhesion molecules in endometrial cancer – A systematic review
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Łukasz Lewczuk, Anna Pryczynicz, Katarzyna Guzińska-UstymowiczAbstractAdhesive molecules are responsible for the cell-cell interaction and the surrounding intercellular environment creating normal tissue architecture. The role of adhesion proteins in cancer refers to angiogenesis, loss of tissue continuity, and deprivation of intercellular contact with the extracellular matrix, promoting the spread of cancer through the formation of metastases. The integrity of the epithelium is disturbed – with disturbances in the whole mechanism of cell connections, thanks to which cancer cells infiltrate surrounding tissues, and move to lymphatic and blood vessels. Adhesive molecules are divided into five main families: cadherin, catenins, integrins, the immunoglobulin superfamily and non-classical adhesion molecules. In the present review we describe the role of all five families of adhesive molecules in endometrial cancer.
  • Overweight in classical phenylketonuria children: A retrospective cohort
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Dariusz Walkowiak, Lukasz Kaluzny, Anna Bukowska-Posadzy, Mariusz Oltarzewski, Rafal Staszewski, Jerzy A. Moczko, Michal Musielak, Jaroslaw WalkowiakAbstractPurposeThis cohort study aimed to determine the frequency of overweight and obesity in classical phenylketonuria children and to identify the possible influence of metabolic control on the BMI of the studied patients.Patients and methodsThe study group included 63 classical phenylketonuria patients (40 girls and 23 boys; aged 5–16 years). Their z-score BMI, metabolic control, educational level of parents and socioeconomic status were determined.ResultsTwenty children were overweight or obese and only three were underweight. The percentages of overweight and obese children were 31.7% for the whole group, 21.7% (5 out of 23) for boys and 37.5% (15 out of 40) for girls. Overweight and obesity in these phenylketonuria patients was statistically significantly more frequent when compared to national reference studies (p = 0.0031).The five-year index of dietary control and the percentage of spikes exceeding 6 and 12 mg/dl (Spikes 6 and 12) indicated better metabolic control in the case of normal weight children than those who were overweight and obese (p 
  • Serum TEM5 and TEM7 concentrations correlate with clinicopathologic
           features and poor prognosis of colorectal cancer patients
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Łukasz Pietrzyk, Paulina WdowiakAbstractPurposeColorectal cancer (CRC) is a serious threat worldwide; therefore, discovering sensitive and specific serum biomarkers for early stages of CRC is a great challenge. In this study, we evaluated whether tumour endothelial marker 5 (TEM5) and 7 (TEM7) circulating in blood serum can be useful as blood-based markers for detection, progression, and prognosis in CRC patients. Moreover, their specificity and sensitivity in the early diagnosis of CRC were compared with common carcinoma diagnostic markers, i.e. CEA and Ca 19-9.Materials and methodsThe study included 45 CRC patients and 35 healthy individuals. The serum concentration of TEM5 and TEM7 were quantified using sandwich ELISA.ResultsThe mean TEM5 and TEM7 serum concentrations were statistically significantly higher in the CRC patients than in the healthy controls. Moreover, the mean TEM5 and TEM7 concentrations were statistically significantly higher in the serum of patients with late stage (III/IV) compared to early stage (I/II) cancer. The TEM5 and TEM7 values increased along the development of the T, N, and M stages. The TEM5 and TEM7 sensitivity and specificity in CRC detection were higher than routinely used blood markers (CEA, Ca19-9). The high TEM5 and TEM7 concentrations were associated with worse overall survival compared to CRC patients of low TEM5 and TEM7 concentrations.ConclusionsTaken together, these findings suggest that TEM5 and TEM7 serum concentrations can be considered as useful biomarkers for the detection of CRC patients and for monitoring cancer progression and identifying patients with a high possibility of poor survival.
  • EZH2, new diagnosis and prognosis marker in acute myeloid leukemia
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Amal Mechaal, Samia Menif, Salem Abbes, Ines SafraAbstractPurposeAcute myeloid leukemia (AML) is a heterogeneous disease. The discovery of novel discriminative biomarkers remains of utmost value for improving outcome predictions. Enhancer of zeste homolog 2 (EZH2) is a histone methyltransferase of H3K27me3. It is frequently up-regulated in human cancers and associated with silencing of differentiation genes. We aimed herein to investigate the prevalence and prognosis impact of somatic EZH2 mutations and their potential associations with other prognostic markers FLT3, NPM1, DNMT3A and IDH2.Materials and methodsOur study population was composed of 211 Tunisian patients with de novo AML and 14 healthy donors. The 11 last exons coding the set domain of EZH2 were investigated by PCR and Sanger sequencing.ResultsEZH2 mutations were identified in 66/211 (31%) patients with a sex ratio of 1.06. The presence of EZH2 mutations was statistically significantly associated with failure consolidation therapy (p = 0.004). There were no differences in the incidence of EZH2 mutations and FLT3-ITD, NPM1, DNMT3A and IDH2 mutations. When EZH2 mutations were associated with those of FLT3 or IDH2, a short duration of progression free survival was observed (p 
  • The role of P2X7 receptor in prognosis and metastasis of colorectal cancer
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Ying Zhang, Jingjing Ding, Lili WangAbstractPurposeColorectal cancer (CRC) is one of the leading causes of cancer mortality in the world. P2X7 receptor (P2X7R), encoded by the P2rx7 gene, is a trimeric ion channel activated by extracellular Adenosine triphosphate and is widely expressed in various types of tissues and tumors to regulate inflammation, cell proliferation, or death. The discovery of new biomarkers and understanding the role of P2X7R in CRC are therefore critical to improving the prognosis and treatment of CRC.Materials and methodsP2X7R expression was analyzed in CRC tumor samples and normal colorectal tissues from 97 patients and various colon cancer cell lines. The correlation of tumor antigens, survival periods, and P2X7R expression were documented.ResultsP2X7RHigh and P2X7RLow populations were observed in CRC patients. P2X7RHigh patients had relatively shorter survival periods, higher levels of serum carcinoembryonic antigen, and greater numbers of advanced tumors. In addition, P2X7R expression had a significant up-regulation in metastatic CRC and metastatic CRC cell lines, which indicates that P2X7R expression is positively associated with metastasis.ConclusionsP2X7R expression might be a potential biomarker for prognosis and metastasis of CRC.
  • The potential role of inflammation in cryptogenic stroke
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Paulina Gąsiorek, Maciej Banach, Agata Sakowicz, Andrzej Głąbiński, Bożena Sosnowska, Marek Maciejewski, Agata Bielecka-DabrowaAbstractPurposeThe study aimed to identify biomarkers predictive of cryptogenic stroke in patients aged
  • Effect of quinolinic acid – A uremic toxin from tryptophan metabolism
           – On hemostatic profile in rat and mouse thrombosis models
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Agnieszka Leszczyńska, Tomasz Misztal, Natalia Marcińczyk, Tomasz Kamiński, Karol Kramkowski, Ewa Chabielska, Dariusz PawlakAbstractPurposeWe aimed to determine the effect of quinolinic acid (QA) on hemostasis in rat and mouse models of thrombosis.Material and methodsWistar rats (male, n = 72) received QA dissolved in drinking water in doses of 3, 10, 30 mg/kg or pure drinking water (vehicle control group -VEH) for 14 days. On the 14th day of the experiment the effect of QA on hemostasis was evaluated using electrically induced arterial thrombosis model. The following parameters were measured: thrombus weight, hematology, thromboelastometric (ROTEM) parameters, TXA2 and 6-keto-PGF1α concentration, coagulation and fibrinolytic markers activity and concentration.GFP mice (male, n = 30) were assigned to the group receiving QA (30 mg/kg) or VEH for 14 days and to the group receiving: single intravenous dose of QA (30 mg/kg) or VEH or the same dose of QA and anti-CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) antibody conjugated with Alexa Fluor 647. The effect of QA on hemostasis was evaluated in the model of laser-induced injury of mesentery vein using intravital confocal microscopy.ResultsAdministering QA for 14 days resulted in a divergent, depending on dose, increase in concentration of active form of tPA and PAI-1 and concentration of total PAI-1 and PAP complexes in rats’ plasma. In turn, administering QA for 14 days in mice revealed its prothrombotic activity, while single-dose IV administration revealed its antithrombotic activity, through the up-regulation of PECAM-1 expression.ConclusionsWe demonstrated the first evidence for the opposite biological effects of QA on hemostasis in rat and mouse thrombosis models.
  • Twenty years of growth hormone treatment in dialyzed children in
           Poland—Results of national multicenter study
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Dominika Adamczuk, Beata Leszczyńska, Piotr Skrzypczyk, Agnieszka Turczyn, Agnieszka Antonowicz, Anna Majcher, Maria Szczepańska, Piotr Adamczyk, Ilona Zagożdżon, Aleksandra Żurowska, Marcin Tkaczyk, Anna Jander, Przemysław Sikora, Anna Wasilewska, Alfred Warzywoda, Katarzyna Kiliś-Pstrusińska, Danuta Zwolińska, Katarzyna Zachwieja, Dorota Drożdż, Roman StankiewiczAbstractPurposeThe aim of the study was to analyze the effect of recombinant human growth hormone (rhGH) therapy and to establish factors influencing growth rate in dialyzed children in Poland.MethodsWe retrospectively analyzed medical records of 81 children with end-stage renal disease (ESRD) on chronic dialysis treated with rhGH for ≥12 months between 1994 and 2014. The following data were recorded: cause of ESRD, dialysis modality, age at the dialysis and rhGH initiation [years]. In addition, growth [cm], [standard deviation score - SDS], body mass index [SDS], skeletal age [years], bone mineral density [SDS], hemoglobin, total protein, albumin, urea, creatinine, calcium, phosphorus, calcium phosphorus product, PTH, and alkaline phosphatase were measured at the baseline and after 12 months.ResultsGrowth velocity in 81 children during one-year rhGH treatment was 7.33 ± 2.63 cm (ΔSDS 0.36 ± 0.43). Height SDS increased significantly (-3.31 ± 1.12 vs. -2.94 ± 1.15, p 
  • Selected elements of extracellular matrix of the skin in diabetes and
           insulin resistance
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Marek Niczyporuk, Małgorzata Knaś, Halina CarAbstractPurposeReconstruction of the skin extracellular matrix is a physiological phenomenon occurring on a continuous basis. The aim of this study is to evaluate the content of basic enzymes preventing oxidative stress: superoxide dismutase 2 and 3 as well as catalase, the content of hyaluronic acid, and the activity of N-acetyl-β-d-hexosaminidase and β-d-glucuronidase in the skin of rats used as animal models of diabetes and insulin resistance, before and after the treatment.Materials and methodsThe study was conducted on a group of sexually mature male Wistar rats divided into 7 groups of 10 animals. Insulin resistance was induced by feeding the rats with a high-fat diet, and diabetes was induced by a single injection of streptozotocin. Chosen groups of rats were treated with insulin or metformin. After 8 weeks, we excised a fragment of shaved dorsal skin from anesthetized rats in each group.ResultsIn the course of diabetes and insulin resistance, an intensified defensive activity of cells against the oxidative stress was observed in the undamaged skin, expressed by an increase in the relative content of superoxide dismutase 2 and 3, catalase and the activity of N-acetyl-β-d-hexosaminidase and β-d-glucuronidase. Diabetes and insulin resistance cause similar skin damage, as there are no differences in the relative contents or specific activities of the examined parameters.ConclusionsInsulin and metformin improve the quality of the skin in rats with diabetes and insulin resistance, by restoring the content of hyaluronic acid to the healthy skin level.
  • Efficacy of daratumumab monotherapy in real-world heavily pretreated
           patients with relapsed or refractory multiple myeloma
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Aleksander Salomon-Perzyński, Adam Walter-Croneck, Lidia Usnarska-Zubkiewicz, Dominik Dytfeld, Patrycja Zielińska, Małgorzata Wojciechowska, Jadwiga Hołojda, Paweł Robak, Anna Pasternak, Wanda Knopińska-Posłuszny, Dorota Hawrylecka, Marcin Wójtowicz, Agnieszka Szeremet, Michał Osowiecki, Monika Mordak-Domagała, Jan Maciej Zaucha, Krzysztof Giannopoulos, Krzysztof Warzocha, Krzysztof JamroziakAbstractPurposeDaratumumab is a promising new agent for relapsed/refractory multiple myeloma (RRMM). However, there are limited data on its clinical activity and tolerability in the real-world patients. The purpose of this study is to determine the efficacy and toxicity profile of daratumumab monotherapy in the real-life setting.Patients and methodsThirty RRMM patients treated with daratumumab who had previously received at least three treatment lines including a proteasome inhibitor and an immunomodulatory drug or had been double refractory (DRMM) were included to the Polish Myeloma Group observational study.ResultsThe objective response rate to daratumumab was 42.8%. Median progression-free survival (PFS) and overall survival reached 9.5 and 13.8 months, respectively. Importantly, patients with DR-MM had a significantly shorter PFS than other patients (median PFS of 4.1 vs. 12.1 months). Daratumumab was generally well tolerated, however two patients had their therapy interrupted due to adverse events.ConclusionDaratumumab monotherapy has significant activity and good tolerance in heavily pretreated RRMM patients.
  • State of the art – sirtuin 1 in kidney pathology – clinical
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Angelika Bazyluk, Jolanta Malyszko, Tomasz Hryszko, Edyta ZbrochAbstractSirtuins represent a group of nicotinamide adenine dinucleotide dependent histone deacetylases, which regulates various biological pathways by promoting chromatin silencing and transcriptional repression. Therefore, they are linked to cellular energy metabolism, mitochondrial biogenesis, stress response, apoptosis, inflammation and fibrosis. Since sirtuin 1 became a promising candidate for targeted therapies of numerous conditions, researchers have been investigating its activator. As for now, natural agents and antidiabetic drug – metformin, have been found to activate sirtuin 1. Sirtuin 1 is able to improve kidney outcomes by direct impact on kidney cells, regulation of non-specific processes generally involved in pathogenesis of age–dependent and metabolic disorders and improvement of the comorbid diseases. This review discusses the state of the art knowledge on the role of sirtuin 1 on kidney pathology.
  • Assessment of serum levels of DIO1 and DIO3 in patients diagnosed with
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Elżbieta Gałecka, Anna Kumor-Kisielewska, Paweł GórskiAbstractPurposeChronic obstructive pulmonary disease is the most common chronic lung disease, which may be caused by different pathological processes, including inflammation. Furthermore, signs of changes in thyroid hormone levels are found in some patients. Deiodinases (DIOs) are selenoproteins (enzymes) involved in the synthesis of thyroid hormones. It has been found that these molecules are involved in inflammatory processes. We carried out this preliminary study to investigate the levels of two deiodinases, i.e. type 1 deiodinase (DIO1) and type 3 deiodinase (DIO3), and their possible association with COPD and specific clinical parameters.Patients and methodsSerum levels of DIO1 and DIO3 as well as lung function parameters were measured in 50 patients suffering from COPD and 30 healthy control subjects. The Mann-Whitney U test and Pearson’s correlation coefficient were used to compare and correlate data.ResultsSerum levels of DIO1 and DIO3 were significantly elevated in COPD patients (97.9 ± 55.6 versus 28.2 ± 28.3 U/L for DIO1 and 19.6 ± 10.7 versus 6.4 ± 6.3 U/L for DIO3; p 
  • Original research article Parainfluenza virus infection enhances
           NSAIDs–induced inhibition of PGE2 generation and COX-2 expression in
           human airway epithelial cells
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Anna Lewandowska-Polak, Małgorzata Brauncajs, Marzanna Jarzębska, Małgorzata Pawełczyk, Marcin Kurowski, Joanna Makowska, Marek L. KowalskiAbstractPurposeRespiratory viral infection and nonsteroidal anti-inflammatory drugs (NSAIDs) may affect arachidonic acid (AA) metabolism in the airway epithelium, however their joint effect has not been studied. We hypothesized, that alternations of AA metabolism in human airway epithelial cells (ECs) – induced by Parainfluenza virus type 3 (PIV3) – may be modified by concomitant treatment with NSAIDs.Materials and methodsNasal (RPMI 2650) and bronchial (BEAS-2B) epithelial cells were cultured into confluence and then infected with PIV3. Prostaglandin E2 (PGE2) and 15-hydroxyeicosatetraenoic acid (15-HETE) levels in cell supernatants were measured by ELISA and expression of cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LO) and 15-lipoxygenase (15-LO) mRNA in cells was evaluated after reverse transcription with real-time polymerase chain reactions.ResultsPGE2 generation was decreased by PIV3 infection in the upper airway epithelial cells, and increased in the lower airway epithelial cells. Both naproxen and celecoxib induced significant reduction in PGE2 release in both infected and non-infected upper and lower airway epithelial cells. However, in PIV3-infected epithelial cells celecoxib inhibited PGE2 release and COX-2 expression to significantly higher degree as compared to non-infected cells. 15-HETE generation or COX-1, 5-LO and 15-LO expression were not affected by the virus infection or by NSAIDs.ConclusionVirus infection in airway epithelial cells enhances inhibitory effect of NSAIDs on prostaglandin E2 generation.
  • microRNA expression profile in Smooth Muscle Cells isolated from thoracic
           aortic aneurysm samples
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Anna Kasprzyk-Pawelec, Anna Wojciechowska, Mateusz Kuc, Jakub Zielinski, Adam Parulski, Mariusz Kusmierczyk, Anna Lutynska, Katarzyna Kozar-KaminskaAbstractPurposeThoracic aortic aneurysm (TAA) is a cardiovascular disease characterized by increased aortic diameter, treated with surgery and endovascular therapy in order to avoid aortic dissection or rupture. The mechanism of TAA formation has not been thoroughly studied and many factors have been proposed to drive its progression; however strong focus is attributed to modification of smooth muscle cells (SMCs). Latest research indicates, that microRNAs (miRNAs) may play a significant role in TAA development – these are multifunctional molecules consisting of 19–24 nucleotides involved in regulation of the gene expression level related to many biological processes, i.e. cardiovascular disease pathophysiology, immunity or inflammation.Materials and methodsPrimary SMCs were isolated from aortic scraps of TAA patients and age- and sex-matched healthy controls. Purity of isolated SMCs was determined by flow cytometry using specific markers: α-SMA, CALP, MHC and VIM. Real-time polymerase chain reaction (RT-PCR) was conducted for miRNA analysis.ResultsWe established an isolation protocol and investigated the miRNA expression level in SMCs isolated from aneurysmal and non-aneurysmal aortic samples. We identified that let-7 g (0.71-fold, p = 0.01), miR-130a (0.40-fold, p = 0.04), and miR-221 (0.49-fold, p = 0.05) significantly differed between TAA patients and healthy controls.ConclusionsFurther studies are required to improve our understanding of the pathophysiology underlying TAA, which may aid the development of novel, targeted therapies. The pivotal role of miRNAs in the cardiovascular system provides a new perspective on the pathophysiology of thoracic aortic aneurysms.
  • Ultrasound images in hepatic alveolar echinococcosis and clinical stage of
           the disease
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Małgorzata Sulima, Wacław Nahorski, Tomasz Gorycki, Wojciech Wołyniec, Piotr Wąż, Iwona Felczak-Korzybska, Beata Szostakowska, Katarzyna SikorskaAbstractPurposeHepatic alveolar echinococcosis (AE) is a parasitic disease caused by the larval stage of the tapeworm Echinococcus multilocularis. Ultrasonography is the method of choice in the initial diagnosis of AE. The aim of the study is to present the most frequent sonomorphological patterns of lesions in hepatic AE based on the analysis of ultrasound findings in patients treated for AE at the University Centre of Maritime and Tropical Medicine (UCMMiT; Gdynia, Poland), and to establish whether there is a relationship between the clinical stage of AE and the occurrence of a specific sonomorphological pattern of hepatic lesions.Patients and methodsWe analysed the results of ultrasound examinations of 58 patients hospitalized in the UCMMiT with probable or certain diagnosis of AE. Liver lesions were assessed according to the classification developed by researchers from the University Hospital in Ulm (Germany). Statistical analysis was based on the relationship between the occurrence of a specific sonomorphological pattern of hepatic lesions and the clinical stage of AE.ResultsThe most frequently observed patterns of AE lesions in the liver were the hailstorm and the pseudocystic patterns. There was no correlation between the clinical stage of the disease and the ultrasonographic appearance of lesions. There was no statistically significant relationship between the more frequent occurrences of specific ultrasonographic patterns of lesions in the liver and radical or non-radical surgery.ConclusionsThe ultrasonographic appearance of the lesion in liver AE cannot determine the therapeutic management. Treatment plan should be established based on the PMN classification.
  • Accuracy of Doppler echocardiography in the hemodynamic assessment of
           pulmonary circulation in patients with systemic sclerosis
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Michał Ciurzyński, Piotr Bienias, Katarzyna Ciesielska, Anna Chrzanowska, Iwona Dudzik-Niewiadomska, Katarzyna Kurnicka, Justyna Domienik-Karłowicz, Maria Siwicka, Piotr Sobieraj, Agnieszka Kalińska-Bienias, Marcin Kurzyna, Piotr PruszczykAbstractPurposePulmonary hypertension (PH) is an ominous complication in systemic sclerosis patients (SSc) and echocardiography is a screening tool for its detection. The goal of this study was to assess the reliability of resting and exercise echo Doppler parameters with data obtained by right heart catheterization (RHC).Material and methodsWe included 91 patients (84 F, 53.3 ± 15.2 years) with SSc. Transthoracic echocardiography followed by exercise Doppler-echocardiography (EDE) were performed. A positive EDE was defined as a ≥20 mmHg increase in tricuspid regurgitation peak gradient (TRPG). RHC with exercise was performed in positive EDE patients and/or in subjects with resting TRPG > 31 mmHg.ResultsFinally, RHC was performed in 20 patients. The correlation for the echocardiography and invasive measurement of systolic (sPAP) and mean (mPAP) pulmonary artery pressure was r = 0.66 (p = 0.001) and r = 0.7 (p = 0.001), respectively. We also found significant correlation between echocardiography and invasive measurement of exercise sPAP r = 0.68 (p = 0.001) and exercise mPAP r = 0.67 (p = 0.002). There was a correlation between pulmonary vascular resistance (PVR) assessed by echocardiography and measured by RHC r = 0.49, p = 0.027. The equation derived within our population was: PVR by echocardiography = 9.6*TRV/TVIRVOT+0.068. We also performed ROC analysis to predict PVR > 2 WU. Our results highlight that sPAP has the highest AUC (0.802, 95% CI 0.585–1).ConclusionDoppler resting and exercise echocardiography may provide a reliable, noninvasive method for determining resting and exercise sPAP, mPAP, and PVR in SSc patients, although it may underestimate or overestimate these values in some individuals. Doppler echocardiography does not replace RHC for definite hemodynamic assessment of suspected PH.
  • Role of endoplasmic reticulum stress and protein misfolding in disorders
           of the liver and pancreas
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Jan Lukas, Jola Pospech, Christina Oppermann, Christina Hund, Katharina Iwanov, Supansa Pantoom, Janine Petters, Moritz Frech, Susanne Seemann, Franziska-Gisela Thiel, Jana-Marielle Modenbach, Robert Bolsmann, Laura de Freitas Chama, Franziska Kraatz, Firas El-Hage, Manuel Gronbach, Annelie Klein, Regina Müller, Sabine Salloch, Frank-Ulrich WeissAbstractThe endoplasmic reticulum (ER) is the site of synthesis and folding of membrane and secretory proteins. The fraction of protein passing through the ER represents a large proportion of the total protein in the cell. Protein folding, glycosylation, sorting and transport are essential tasks of the ER and a compromised ER folding network has been recognized to be a key component in the disease pathogenicity of common neurodegenerative, metabolic and malignant diseases. On the other hand, the ER protein folding machinery also holds significant potential for therapeutic interventions. Many causes can lead to ER stress. A disturbed calcium homeostasis, the generation of reactive oxygen species (ROS) and a persistent overload of misfolded proteins within the ER can drive the course of adisease. In this review the role of ER-stress in diseases of the liver and pancreas will be examined using pancreatitis and Wilson´s disease as examples. Potential therapeutic targets in ER-stress pathways will also be discussed.
  • Clinical importance of sex differences in dyspnea and its sex related
           determinants in asthma and COPD patients
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Szymon Skoczyński, Jan Zejda, Grzegorz Brożek, Klaudia Glinka, Sylwia Waz, Beata Kotulska, Adam BarczykAbstractPurposeThere is an increasing amount of data regarding the influence of sex on dyspnea perception, however, the influence of sex has not been included in clinical guidelines for asthma and chronic obstructive pulmonary disease (COPD).Materials and methodsThe study included 123 clinically stable subjects. Seventy five patients had COPD, of which 70.6% were men and 29.4% were women. Forty-eight subjects had asthma, comprised of 45.8% and 54.2%, men and women, respectively. Dyspnea was assessed with the use of modified Medical Research Council (mMRC) Visual Analogue Scale VAS, and BORG scale. All patients underwent spirometry with a broncho-reversibility test.ResultsThere were no differences in age for neither asthma patients, 56.5 ± 11.6 and 55.0 ± 12.7 (p = 0.5) for males and females, nor for COPD patients, 66.8 ± 9.0 and 66.8 ± 7.7 (p = 0.7) for males and females, respectively. Asthmatic females had more dyspnea than males when assessed with VAS, 1.85 ± 2.24 and 3.84 ± 2.80 (p = 0.01), for males and females, respectively. When assessed with BORGpre 6-MWT, dyspnea results were 0.86 ± 1.83 and 2.43 ± 2.31 (p = 0.005), for males and females, respectively. In the whole group, apart from FEV1 (for mMRC, VAS, BORGpre) and BMI (BORGpost) the severity of dyspnea was related to female sex when assessed with mMRC (OR=2.83; 95%CI: 1.25–6.42) and VAS (OR = 2.17; 95%CI:1.00–4.73).ConclusionsAlthough more apparent in asthma, it was revealed for the first time, that sex has a strong influence on the magnitude of dyspnea perception, both in asthma and COPD. Therefore, sex related dyspnea sensation should probably be included in clinical assessment and patient treatment.
  • Diagnostic accuracy of frontotemporal dementia. An artificial
           intelligence-powered study of symptoms, imaging and clinical judgement
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Maksymilian A. Brzezicki, Matthew D. Kobetić, Sandra Neumann, Catherine PenningtonAbstractPurposeFrontotemporal dementia (FTD) is a neurodegenerative disorder associated with a poor prognosis and a substantial reduction in quality of life. The rate of misdiagnosis of FTD is very high, with patients often waiting for years without a firm diagnosis. This study investigates the current state of the misdiagnosis of FTD using a novel artificial intelligence-based algorithm.Patients & MethodsAn artificial intelligence algorithm has been developed to retrospectively analyse the patient journeys of 47 individuals diagnosed with FTD (age range 52–80). The algorithm analysed the efficiency of patient pathways by utilizing a reward signal of ‒1 to +1 to assess the symptoms, imaging techniques, and clinical judgement in both behavioural and language variants of the disease.ResultsOn average, every patient was subjected to 4.93 investigations, of which 67.4% were radiological scans. From first presentation it took on average 939 days for a firm diagnosis. The mean time between appointments was 204 days, and the average patient had their diagnosis altered 7.37 times during their journey. The algorithm proposed improvements by evaluating the interventions that resulted in a decreased reward signal to both the individual and the population as a whole.ConclusionsThe study proves that the algorithm can efficiently guide clinical practice and improve the accuracy of the diagnosis of FTD whilst making the process of auditing faster and more economically viable.
  • Can hepatokines be regarded as novel non-invasive serum biomarkers of
           intrahepatic lipid content in obese children'
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Marta Flisiak-Jackiewicz, Anna Bobrus-Chociej, Natalia Wasilewska, Eugeniusz Tarasow, Malgorzata Wojtkowska, Dariusz Marek LebensztejnAbstractPurposeHepatokines are proteins produced by the liver and involved in regulating glucose and lipid metabolism. However, their role as the biomarkers of intrahepatic lipid content is not clear. The aim of the study was to evaluate the serum concentration of selected hepatokines: fibroblast growth factor-21 (FGF-21), selenoprotein P (SELENOP) and sex hormone-binding globulin (SHBG) in obese children.Patients and methodsThe cross-sectional study included 86 obese children with suspected liver disease. Nonalcoholic fatty liver disease (NAFLD) was diagnosed in children with liver steatosis in ultrasound with elevated alanine aminotransferase (ALT) serum activity and excluded other liver diseases. The total intrahepatic lipid content (TILC) was assessed by magnetic resonance proton spectroscopy (1H-MRS).ResultsThe concentration of FGF-21 and SELENOP was significantly higher and SHBG significantly lower in children with NAFLD compared to controls. Only FGF-21 level was significantly higher in NAFLD children than in obese patients without NAFLD. The significant positive correlation of FGF-21 with ALT, gamma glutamyltransferase (GGT), triglycerides, homeostatic model assessment–insulin resistance (HOMA-IR), the degree of liver steatosis in ultrasound and TILC in 1H-MRS were found. The ability of serum FGF-21 to diagnose severe liver steatosis was significant.ConclusionsFGF-21 can be considered as a suitable biomarker in predicting TILC and fatty liver in obese children.
  • Transcriptional activity of epigenetic remodeling genes declines in
           keratinocytes after in vitro expansion
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Jolanta Kamińska, Paulina Langa, Milena Deptuła, Jacek Zieliński, Paweł Sachadyn, Anna Wardowska, Michał PikułaAbstractPurposeIn vitro expansion is an invaluable method to obtain keratinocytes in amounts necessary for effective transplantation therapies. In vitro cell culturing provokes questions concerning potential epigenetic alterations occurring in expanded cells in the context of usefulness for transplantation and safety. The purpose of this study was to investigate as to whether keratinocyte expansion is associated with changes in the activity of genes responsible for the maintenance of epigenetic stability.Materials and methodsWe focussed on the transcriptional activity of genes involved in different epigenetic mechanisms including DNA methylation and histone modifications. We used quantitative real-time PCR to determine transcript levels of 16 epigenetic remodelling markers in 14 patients in the epidermal cells directly after collection and after in vitro expansion.ResultsWe observed a remarkable decline in the transcriptional activity of the epigenetic remodelling genes following in vitro expansion, while no further fall of expression with passaging. In whole skin, we found even higher expression levels of the epigenetic markers.ConclusionsTransmission to in vitro environment challenges cellular signalling and metabolism. The regulation of epigenetic remodelling maintains the balance between cellular plasticity and phenotype deviation. This preliminary research demonstrated reduced activity of genes responsible for epigenetic modifications of DNA and histones in in vitro expanded epidermal cells. This observation indicates that epigenome re-patterning in cultured epidermal cells is significantly less intensive than in the skin. Also, this observation may imply that after adaptation to in vitro conditions, the epigenome does not undergo extensive transformation during further cultivation.
  • Contribution of sialic acids to integrin α5β1 functioning in
           melanoma cells
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Ewa Kolasińska, Marcelina E. Janik, Anna Lityńska, Małgorzata PrzybyłoAbstractPurposeTo establish the relationship between sialylation of integrin α5β1 and possible alteration in the function of α5β1 receptor in melanoma cells.Materials and methodsIntegrin α5β1 was isolated from primary WM115 (RGP/VGP-like phenotype) and metastatic WM266-4 (lymph node metastasis) cells via affinity chromatography. Integrin α5β1 sialylation and the shift in relative masses of the enzymatically desialylated subunits were confirmed by confocal microscopy and SDS-PAGE, respectively. The ELISA assay was performed to evaluate sialic acid (SA) influence on integrin α5β1 binding to fibronectin (FN). Cell invasion was investigated by the Transwell invasion assay. The effect of neuraminidases treatment on melanoma cells was assessed by flow cytometry using Maackia amurensis and Sambucus nigra lectins.ResultsBoth subunits of integrin α5β1 were found to be more abundantly sialylated in primary than in metastatic cells. The removal of SA had no effect on the purified integrin α5β1 binding to FN. Although metastatic cells underwent more pronounced desialylation than primary cells, invasion of primary WM115 cells was more dependent on the presence of α2-3 linked SA than it was in the case of metastatic WM266-4 cells. In both melanoma cell lines not only integrin α5β1 was involved in invasion, however simultaneous desialylation and usage of anti-integrin α5β1 antibodies resulted in lower invasion abilities of primary WM115 cells.ConclusionsOur data suggest that in primary melanoma cells integrin α5β1 action is more likely dependent on its glycosylation profile, i.e. the presence of SA residues, which influence (decreased) their invasion properties and may facilitate malignant melanoma progression.
  • IL-1α and IL-6 predict vascular events or death in patients with cerebral
           small vessel disease—Data from the SHEF-CSVD study
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Jacek Staszewski, Ewa Skrobowska, Renata Piusińska-Macoch, Bogdan Brodacki, Adam StępieńAbstractPurposeThe natural clinical course of cerebral small vessel disease (CSVD) was not thoroughly described. The aim of this single center cohort study was to establish biochemical predictors of vascular events and death in CSVD patients during a 24-month follow-up.Patients and methodsA total of 130 functionally independent patients with marked MRI features of CSVD and recent lacunar stroke (n = 52,LS), vascular Parkinsonism (n = 28,VaP) or dementia (n = 50,VaD) were prospectively recruited. Serum markers of endothelial dysfunction, inflammation and hemostasis were determined at baseline. The primary outcome was defined as occurrence of death or any vascular events during the observation.ResultsThe mean age was 72 ± 8.1 years, and 37.6% of the patients were women. The mean follow-up time was 22.3 ± 4.3 months, and 84.6% of patients had extensive white matter lesions on baseline MRI. The overall mortality rate was 6.9%, and vascular events or death occurred in 27% of the patients. Kaplan-Meier survival curves revealed no significant differences between CSVD groups (log rank p = 0.49). Cox regression analysis revealed that IL-1α (HR 1.4; 95%CI 1.09–1.8), IL-6 (1.4;1.1–2.2), hs-CRP (1.1;1.06–1.9), homocysteine (1.4;1.1–1.8), fibrinogen (1.4;1.05–2), and d-dimer (2.7;1.6–4.5) were significantly associated with the primary outcome. IL-1α (1.3;1.07–1.8), IL-6 (1.4;1.02–2.2), d-dimer (2.8;1.6–5) and homocysteine (1.4;1.1–1.8) remained significant after adjusting for age, sex and CSVD radiological markers.ConclusionsOur study demonstrated the important prognostic role of various circulation markers of inflammation in individuals with different clinical signs and radiological markers of CSVD. The strongest association occurred between IL-1α, IL-6 and recurrent stroke, other vascular events and death.
  • Novel potential biomarkers for pancreatic cancer – A systematic
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Ala Litman-Zawadzka, Marta Łukaszewicz-Zając, Barbara MroczkoAbstractBackgroundIt is estimated that in developed countries the incidence rate of pancreatic cancer (PC) will continue to rise and by 2020 will be the second most fatal cancer. The mortality of PC patients closely parallels the incidence rate, as this malignancy remains asymptomatic until it reaches an advanced stage of disease. Thus, novel biochemical markers that improve the management of PC patients are necessary. The aim of the work that follows is to investigate whether selected inflammatory mediators might be used in the diagnosis of PC, with the aim of improving the prognosis for PC patients.MethodsWe performed a thorough search for literature pertaining to our investigation via the MEDLINE/PubMed database.ResultsIt has been proved that certain inflammatory mediators might be involved in tumor progression, such as growth, proliferation, migration and angiogenesis of tumor cells. In the present review, we summarized and referred to a number of original papers concerning the clinical significance of selected cytokines and specific inflammatory proteins such as C-reactive protein, as well as of various matrix metalloproteinases and their tissue inhibitors, as potential biomarkers for PC in comparison to well-established tumor markers for this malignancy.ConclusionPresented proteins might be potential biomarkers in the diagnosis and progression of PC.
  • Clinical characteristics of autoimmune pancreatitis with IgG4 related
           kidney disease
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Sawako Kuruma, Terumi Kamisawa, Masataka Kikuyama, Kazuro Chiba, Ryoko Shimizuguchi, Satomi Koizumi, Taku TabataAbstractPurposeTo clarify the clinical characteristics of autoimmune pancreatitis (AIP) in immunoglobulin (Ig)G4-related kidney disease (IgG4-RKD).Patients and methodsA total of 92 patients with AIP were divided into an IgG4-RKD-positive group (RKD-P group, n = 13) and an IgG4-RKD-negative group (RKD-N group, n = 79) on the basis of the diagnostic criteria for IgG4-RKD. Clinical characteristics, including: age; sex; the presence of extrapancreatic lesions other than renal lesions, proteinuria, and hematuria; serum concentrations of IgG, IgG4, IgE, and creatinine; and urinary concentrations of liver-type fatty acid binding protein, α1-microglobulin, β2-microglobulin, and N-acetyl-β-d-glucosaminidase were compared between the RKD-P and RKD-N groups. The clinical course of the RKD-P group was also characterized.ResultsThe prevalence of extrapancreatic lesions other than renal lesions was significantly higher in the RKD-P group (84.6% vs 43.0%,p < 0.01). Serum creatinine (1.19 mg/dl versus 0.74 mg/dl, p < 0.05), urinary β2-microglobulin (6609.8 μg/l vs 265.8 μg/l, p < 0.05), and the prevalence of proteinuria (30.7% vs 7.6%, p < 0.05) were significantly higher in the RKD-P group. Nine out of thirteen patients in the RKD-P group had multiple low-density renal lesions on enhanced computed tomography, 3 patients had multiple high-intensity lesions on diffusion-weighted magnetic resonance images, and 1 patient had diffuse thickening of the renal wall, with a smooth intra-luminal surface.ConclusionsPatients who had AIP with IgG4-RKD were more likely to have extrapancreatic lesions other than those in the kidney, and their serum creatinine and urinary β2-microglobulin concentrations were significantly higher than in those without IgG4-RKD.
  • Exercise training program in patients with NYHA III class systolic heart
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Edyta Smolis-Bąk, Tomasz Chwyczko, Ilona Kowalik, Anna Borowiec, Aleksander Maciąg, Hanna Szwed, Rafał DąbrowskiAbstractPurposeThe aim of this study was to assess exercise capacity and echocardiographic parameters in patients with heart failure with reduced ejection fraction (HFrEF) in NYHA III functional class, after cardiac resynchronization therapy (CRT) or implantable cardioverter-defibrillator (ICD) implantation followed by 6 months of supervised rehabilitation in ICD patients.Materials and methodsThe study included patients with HFrEF and impaired left ventricle systolic function (LVEF ≤ 35%), divided into two groups: CRT group - patients after CRT-D implantation > six weeks, and ICD-rehab group - patients after ICD implantation > six weeks, followed by 6 months of supervised aerobic interval training and the conditioning exercises. At baseline and after 6 months in all the patients cardiopulmonary exercise tests (CPX) and standard echocardiographic examinations were performed.ResultsThe study included 61 patients (49–77 years) with HFrEF. At baseline, the values of CPX parameters were similar in both groups. After completing training almost all CPX parameters in the ICD-rehab group significantly improved, except for anaerobic threshold (AT). In the CRT group significant improvements were found in 2 parameters: peak oxygen uptake (VO2) and exercise tolerance (metabolic equivalents, METs). Significant reductions in left and right ventricle diameters and an increase in LVEF were observed in both groups after 6 months.ConclusionsSignificant improvement in exercise tolerance capacity and increase of LVEF were observed in similar extent both in heart failure patients with CRT and with ICD undergoing the rehabilitation program. Regular, controlled exercise trainings provided additional, safe and easy to conduct therapeutic option for heart failure patients with no indications for CRT.
  • Significance of BRCA1 expression in breast and ovarian cancer patients
           with brain metastasis – A multicentre study
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Monika Szarszewska, Anna Markowska, Robert Jach, Andrzej Marszałek, Violetta Filas, Wiesława Bednarek, Anita Olejek, Piotr Tomczak, Stefan Sajdak, Ewa Nowak-Markwitz, Karolina Jaszczyńska-Nowinka, Joanna Stanisławiak-Rudowicz, Anna Gryboś, Anita Chudecka-Głaz, Marian Gryboś, Krystyna Adamska, Rodryg Ramlau, Janina Markowska, Paweł KnappAbstractPurposeCerebral metastases develop in 10–30% of patients with breast cancer (BC) and in around 3.3 to 4% of patients with ovarian cancer (OC). The aim of the multicenter study is to investigate the correlation between the expression of estrogen alpha receptors (ERα), progesterone receptors (PR), human epidermal growth factor receptor 2 (HER2), stromal cell-derived factor 1 (SDF1) and its receptor C-X-C chemokine receptor type 4 (CXCR4), breast cancer metastasis suppressor 1 (BRMS1), astrocyte elevated gene 1 (AEG1), depending on the status of BRCA1 protein, in patients suffering from OC and BC with brain metastases.Patients and methodsThe analysis included 51 patients: 29 with BC and 22 with OC, in whom brain metastases were disclosed.ResultsIn most patients (65.5% of BC patients and 68.2% of patients with OC tumors) BRCA1 protein loss was found. No correlation was disclosed between the levels of ERα, PR receptors, HER2, SDF1, CXCR4, AEG1, BRMS1 and BRCA1 status, patient age, stage of disease advancement, grade of histological maturity of the cells, presence of metastases to lymph nodes. A statistically significant correlation was disclosed between the negative expression of PR receptors and a high expression of CXCR4 in patients with BC. High values of the AEG1 protein (linked to metastases) were detected alongside a high expression of BRMS1 (a suppressor of metastases).ConclusionsPatients with BC and OC and brain metastases have a frequent loss of BRCA1 expression. The role of ERα, PR, HER2, SDF1, CXCR4, AEG1, BRMS1 in metastatic process needs further studies.
  • Analysis of chosen SNVs in GPC5, CD58 and IRF8 genes in multiple sclerosis
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Monika Chorąży, Natalia Wawrusiewicz-Kurylonek, Renata Posmyk, Agata Zajkowska, Katarzyna Kapica-Topczewska, Adam Jacek Krętowski, Jan Kochanowicz, Alina KułakowskaAbstractPurposeMultiple sclerosis (MS) is an autoimmune disease of the central nervous system with a neurodegenerative compound. Heterogenetic background of autoimmunity pathway components has been suggested in the MS pathogenesis. The main aim of our study was to evaluate the association between selected polymorphisms of theCD58, IRF8 and GPC5 genes and treatment effectiveness in a group of relapsing-remitting MS patients. This is the first study of MS patients from Podlaskie Region in the Polish population.Materials and methodsThe study group comprised 174 relapsing-remitting MS patients diagnosed under 40 years of age. Genotyping was performed using ready to use TaqMan assays.ResultsWe demonstrate a strong association of the polymorphisms with sex, age of onset and response to the treatment applied. A significant correlation was observed in the presence of allele T of rs10492503 polymorphism inGPC5 gene with sex and age of MS onset. Logistic regression analysis revealed an increased risk of the interaction of rs17445836 in IRF8 gene with male sex and the type of treatment (OR = 3.80, p 
  • Circadian clock genes and circadian phenotypes in patients with myocardial
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Ivana Škrlec, Jakov Milić, Marija Heffer, Jasenka Wagner, Borut PeterlinAbstractPurposeHuman physiological activities and diseases are under the control of the circadian rhythm. There are strong epidemiological associations between disrupted circadian rhythms, sleep duration and diseases. Sleep disorders are associated with vascular outcomes, such as myocardial infarction (MI).MethodsWe conducted an association study of genotype-phenotype interaction, to determine which circadian clock gene variants might be associated with the circadian phenotypes in patients with MI. In the present study, we analyzed the allele frequencies of 10 single nucleotide polymorphisms in four circadian clock genes in two independent samples: MI patients and controls. Chronotype was assessed using the Morningness - Eveningness Questionnaire (MEQ) and daytime sleepiness using the Epworth Sleepiness Scale (ESS).ResultsChronotype was associated with the ARNTL genetic variant rs12363415 in MI patients. The polymorphisms rs11932595 of the CLOCK gene and rs934945 of the PER2 gene were associated with daytime sleepiness in the patient group.ConclusionOur data suggest that genetic variations in some circadian clock genes might be related to circadian phenotype (i.e., chronotype and daytime sleepiness) in patients with myocardial infarction.
  • Anti-angiogenic efficacy in invasive breast carcinoma patients depends on
           clinicopathological determinants
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Elżbieta Zarychta, Piotr Rhone, Kornel Bielawski, Małgorzata Michalska, Danuta Rość, Barbara Ruszkowska-CiastekAbstractPurposeThe biggest problem with the occurrence of breast cancer is late diagnosis, which is associated with high mortality rates. The aim of the study was to appraise the number of circulating endothelial precursors and the concentration of vascular endothelial growth factor A (VEGF-A) and the soluble forms of its receptors, sVEGFR1 and sVEGFR2, in breast cancer patients with respect to clinicopathological features.Material and methodsThe study involved 85 women of Caucasian ethnicity aged 45–66 with primary breast cancer without distant metastases (M0). Inclusion criteria were as follows: histopathological examination confirming the diagnosis of primary breast cancer, without previous radiotherapy and chemotherapy. Immunohistochemistry evaluation of oestrogen and progesterone receptors, human epidermal growth factor receptor 2, Ki67 expression was made in all cases. In the EDTA-plasma, the concentrations of VEGF-A and its soluble receptors, sVEGFR1 and sVEGFR2, were measured applying immunoassay techniques. Circulating endothelial progenitor cells (EPCs) were identified with the immune-phenotype CD45−, CD34+, CD133+, CD31+ using flow cytometry.ResultsOlder women with breast cancer had significantly higher concentrations of VEGF-A as well as sVEGFR2 compared with their younger counterparts. A significantly higher concentration of the soluble form of VEGF receptor type 1 in patients with T1 breast cancer in relation to T2 cases was noted. Also, negative correlations between circulating EPCs and histological grading as well as a soluble form of VEGFR2 with histological grading of breast cancer according to the Elston-Ellis classification were observed.ConclusionsAnti-angiogenic potential is divergent in relation to the clinicopathological determinants.
  • Hepcidin – Potential biomarker of contrast-induced acute kidney injury
           in patients undergoing percutaneous coronary interventions
    • Abstract: Publication date: September 2019Source: Advances in Medical Sciences, Volume 64, Issue 2Author(s): Jolanta Malyszko, Hanna Bachorzewska-Gajewska, Jacek S. Malyszko, Ewa Koc-Zorawska, Joanna Matuszkiewicz-Rowinska, Slawomir DobrzyckiAbstractPurposeContrast-induced acute kidney injury (CI-AKI) is a common and potentially serious complication of percutaneous coronary interventions (PCI). In this study, we tested the hypothesis whether serum and urinary hepcidin could represent early biomarkers of CI-AKI in patients with normal serum creatinine undergoing PCI. In addition, we assessed serum and urinary neutrophil gelatinase-associated lipocalin (NGAL), cystatin C, eGFR and serum creatinine in these patients.MethodsSerum and urinary hepcidin and NGAL, serum cystatin C, were evaluated before, and after 2, 4, 8, 24 and 48 h after PCI using commercially available kits. Serum creatinine was assessed before, 24 and 48 h after PCI.ResultsWe found a significant rise in serum hepcidin as early as after 4 and 8 h when compared to the baseline values. Serum NGAL increased after 2, 4 and 8 h, and in urinary NGAL after 4, 8 and 24 h after PCI. We found a significant fall in urinary hepcidin after 8 and 24 h after PCI. Serum cystatin C increased significantly 8 h after PCI, reaching peak 24 h after PCI and then decreased after 48 h. The prevalence of CI-AKI was 8%. Urine hepcidin was significantly lower 8 and 24 h after PCI in patients with CI-AKI, while serum and urine NGAL were significantly higher in patients with CI-AKI.ConclusionsOur findings suggest that serum hepcidin might be an early predictive biomarker of ruling out CI-AKI after PCI, thereby contributing to early patient risk stratification. However, our data needs to be validated in large cohorts with various stages of CKD.
  • Influence of copper (I) nicotinate complex and autophagy modulation on
           doxorubicin-induced cytotoxicity in HCC1806 breast cancer cells
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Mohamed A. Abdel-Mohsen, Camelia A. Abdel Malak, Eman S. El- ShafeyAbstractPurposeDoxorubicin is regarded as the most therapeutic active agent available for triple-negative breast cancer (TNBC) treatment. However, the development of drug resistance and toxicity limits its effectiveness. Thus, developing novel strategies for TNBC treatment remains a significant challenge and doxorubicin-based combinations either by metal complexes (Copper I nicotinate complex) or with autophagy modulators could provide novel strategies and alternative strategies contributed to cancer cell death pathways, autophagy and apoptosis.Materials and methodsThe viability of HCC1806 TNBC cells and IC50 values of Doxorubicin (DOX), Torin-1 (TOR), Chloroquine (CQ) and Copper (I) nicotinate complex (CNC) were assessed by MTT assay. ELISA was used for detecting microtubule-associated protein 1 light chain 3 (LC3) level. Real time PCR was used to determine (NBR1) gene expression. Cell cycle analysis and quantitative detection of acid vesicular organelles (AVOs) was performed by flow cytometry. TOR and CQ were used as autophagy modulators for induction and suppression of autophagy, respectively.ResultsThe half-maximal inhibition effect of TOR combination with DOX was revealed to the induction of autophagic cell death and apoptotic cell death. On the other hand, combination of CQ with DOX increased the growth inhibitory effect, induced accumulation of AVOs and suppressed apoptotic cell death. However, combination of CNC with DOX inhibited autophagy and induced cell cycle arrest.ConclusionDoxorubicin drug based combinations either with TOR, CQ or CNC could positively affect DOX effectiveness and reduce DOX doses applied on HCC1806 cells through modulation of autophagy.
  • Insulin-like growth factor-binding protein 7 (IGFBP 7) as a new biomarker
           in coronary heart disease
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Anna Lisowska, Przemysław Święcki, Małgorzata Knapp, Monika Gil, Włodzimierz J. Musiał, Karol Kamiński, Tomasz Hirnle, Agnieszka TycińskaAbstractPurposeThe role of insulin-like growth factor-binding protein-7 (IGFBP-7) in atherosclerosis is still not well-known. The objective of this study was to find out the following: 1) whether IGFBP-7 may act as a biomarker of coronary artery disease (CAD) occurrence and extent; 2) whether IGFBP-7 is potentially related to the classical and new markers of cardiovascular risk (carotid intima-media thickness - cIMT); 3) whether IGFBP-7 may be a marker of mortality in the group of patients with myocardial infarction (MI).Materials/MethodsThe study group consisted of 212 patients with MI and 75 patients with stable CAD, the control group included 100 healthy volunteers. IGFBP-7 serum concentration was measured.ResultsIGFBP-7 value was considerably higher in the study group (MI and CAD patients - 35.1 ng/ml (P = 0.000001) and 32.7ng/ml (P = 0.0001), respectively), than in the controls – 25.2ng/ml. No statistically significant differences between IGFBP-7 concentrations in the MI and CAD group were found. No relationship between IGFBP-7 and the coronary lesions advancement in the study group was observed. No changes in IGFBP-7 concentration in the MI patients during hospitalization were observed. In the group of MI patients who died during follow-up, a considerably higher cIMT values were found whereas no statistically significant difference was observed in relation to IGFBP-7 (34.6 vs. 35.2 ng/ml).ConclusionsIGFBP-7 is a good biomarker of CAD occurrence but not of its advancement. We demonstrated the existence of the relation between higher IGFBP-7 concentration and the selected classical risk factors of cardiovascular events as well as cIMT values. IGFBP-7 cannot serve as a marker of acute ischemia. Also, IGFBP-7 was not confirmed as a predictor of mortality in the MI patients.
  • Urinary procollagen III aminoterminal propeptide and β-catenin – New
           diagnostic biomarkers in solitary functioning kidney'
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Katarzyna Taranta-Janusz, Anna Moczulska, Hanna Nosek, Joanna Michaluk-Skutnik, Mark Klukowski, Anna WasilewskaAbstractPurpose: We aimed at evaluating urinary levels of procollagen III aminoterminal propeptide (PIIINP) and β-catenin and the relationship between these markers and clinical and laboratory variables in children with a solitary functioning kidney (SFK).Patients and methods: The study group consisted of 98 (M/F: 62/36) children with an SFK with a median age of 8 years. An age-matched control group contained 54 healthy peers. Urinary levels of procollagen III aminoterminal propeptide and β-catenin were measured using a commercially available immunoassay kit.Results: The urinary values of PIIINP (UPIIINP) were significantly increased in patients with SFK versus controls (p  0.05). Only urinary PIIINP levels were correlated to renal function tests, such as serum creatinine, urea, uric acid, and estimated glomerular filtration rate (p
  • Ensembling rules in automatic analysis of pressure on plantar surface in
           children with pes planovalgus
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Marcin Derlatka, Mikhail Ihnatouski, Marek Jałbrzykowski, Vladimir Lashkovski, Łukasz MinarowskiAbstractPurposeThis paper presents a method of ensembling rules obtained through induction of several basic types of decision trees.Material and methodsThe proposed method uses rules generated by means of well-known decision trees: CART, CHAID, exhaustive CHAID and C4.5. The method was tested on data describing pressure distribution under foot during gait in children with pes planovalgus (PV) and typical foot. Children with pes planovalgus underwent surgical intervention and were re-examined. Overall, 316 gait cycles have been used in analysis.ResultsThe obtained results consist of a set of rules for all considered cases and show that the proposed method may be a useful tool of gait analysis on the basis of parameters that have a physical interpretation.ConclusionsThe presented method for mining rules useful in this respect may be successfully used by persons with a typically medical knowledge and could improve the understanding of the human gait phenomenon. There is obviously no reason why this method could not be used in the case of other data as well.
  • Evaluation of the serum prooxidant-antioxidant balance before and after
           vitamin D supplementation in adolescent Iranian girls
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Ameneh Timar, Maryam Saberi-Karimian, Hamideh Ghazizadeh, Seyed Mohammad Reza Parizadeh, Reihaneh Sabbaghzadeh, Maryam Emadzadeh, Fatemeh Eshaghi, Shima Tavallaie, Gordon A. Ferns, Majid Ghayour-MobarhanAbstractPurpose: Oxidative stress is caused by an imbalance between the antioxidant defenses and pro-oxidant production in favor of pro-oxidant production. Vitamin D has the potential for both pro- and anti-oxidative effects. The aim of this study was to assess the effect of high dose vitamin D supplementation on the prooxidant-antioxidant balance (PAB) in Iranian girls attending High School.Materials and methods: A total of 464 girls aged 12–18 years were asked to take vitamin D capsules containing 50000IU vitamin D3 once a week for a period of 9 weeks. All variables were determined at baseline and after 9 weeks of intervention. Fasting blood samples were taken from all subjects. The serum levels of 25OHD were measured using an electrochemiluminescence method. Serum PAB levels were determined using an ELISA reader at a wavelength of 450 nm.Results: Vitamin D supplementation was associated with an increase in serum PAB (P 
  • Serotonin transporter gene linked polymorphism (5-HTTLPR) determines
           progredience of alcohol dependence in Belarusian young males
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Andrei Kapitau, Inessa Goloenko, Victor Obyedkov, Kirill Pavlov, Sławomir Dariusz Szajda, Napoleon WaszkiewiczAbstractPurposeAllelic duality and functional impact of degenerate repeat at 5′- flanking promoter region in SLC6A4 gene of the serotonin transporter (5-HTTLPR), have been in the focus of investigations over the years. Various outcomes regarding an association of its polymorphism with risks of alcohol dependence syndrome (ADS) were presented. Such studies have not been conducted in the Eastern European population e.g. Belarus. We therefore checked: the association of 5-HTTLPR polymorphism with ADS, and functional impact of the polymorphism on progredience of ADS in Belarusian population.Material and methodsThe study involved 499 Belarusian males: 377 subjects with ADS (AG), and a control group (CG) with 122 subjects without alcohol-related problems. The ADS group was further divided into two groups of individuals with rapid (AG (R)) and delayed (AG (D)) progression of ADS. Clinical diagnosis was carried-out using ICD-10 criteria, Belarusian Addiction Severity Index, “B-ASI” and Alcohol-Use-Disorders-Identification-Test (AUDIT). PCR-RFLP analysis was performed.ResultsThere were no significant differences in the distribution of frequencies of either the 5-HTTLPR genotype or the short and long allele among AG and CG. However, the ADS 5-HTTLPR genotype and allele distribution frequencies differ significantly by the variation in progression of ADS.ConclusionsThere is no significant association between polymorphism of serotonin transporter gene and risk of ADS. However, the polymorphism significantly determines progredience of ASD in subjects with pathological patterns of alcohol consumption. Findings from this study carry preliminary significance as a facility to effective alcohol addiction treatment, rehabilitation and preventive services in the Eastern Europe.
  • Comparison of left side or right side vagotomy in the rat subjected to
           acute pancreatitis
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Joanna Szklarczyk, Michalina Kot, Joanna Bonior, Zbigniew Śliwowski, Romana Tomaszewska, Jolanta JaworekAbstractPurposeWe aimed to evaluate the effects of unilateral vagotomy (right-VR or left-VL) on the severity of caerulein-induced acute pancreatitis (AP).Material and methodsVR or VL was done in Wistar rats 4 days before AP, except in control, sham operated group. Following 5 h administration of subcutaneous injections of caerulein, the pancreatic blood flow (PBF), serum lipase and IL-10 in caval blood samples were measured. The pancreatic specimens were taken from sacrificed rats for the assessment of MDA-4-HNE and morphology.ResultsPBF decreased from 310 ± 20 ml/min/100 g of tissue in control rats to 130 ± 12 units in AP (p 
  • Personal mobile device-based pocket echocardiograph—The diagnostic
           value and clinical utility
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Paulina Wejner-Mik, Jarosław D. Kasprzak, Dominika Filipiak-Strzecka, Dawid Miśkowiec, Adrianna Lorens, Piotr LipiecAbstractPurposeA microUSB ultrasound probe, which can be connected to a personal mobile device constitutes a new class of diagnostic pocket-size imaging devices (PSID). The aim of this study was to assess the feasibility and diagnostic value of brief transthoracic echocardiographic examination (bTTE) performed with the use of such equipment.Material and methodsThe study population comprised 87 consecutive patients (58 men, mean age 61 ± 16 years), 53 of whom were admitted to intensive cardiac care unit and 34 patients, who were referred for transthoracic echocardiography from outpatient clinic. All patients underwent bTTE performed by cardiologist with the use of personal mobile device-based PSID. Within 18 h of bTTE all subjects underwent a standard TTE (sTTE) using a full sized echocardiograph by expert echocardiographer.ResultsIn all patients, PSID imaging provided sufficient diagnostic image quality. Echocardiographic measurements were completed for both bTTE and sTTE in 98% of patients. The linear measurements obtained during bTTE showed good to excellent correlation with sTTE results (r = 0.65–0.98; p 
  • An endoscopic training and assessment model for argon plasma coagulation
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Vinay Chandrasekhara, Daniel Rhoades, Pavlos Z. Kaimakliotis, Sun-Chuan Dai, Michael L. KochmanAbstractPurposeArgon plasma coagulation (APC) is a standard modality for the treatment of gastrointestinal bleeding. However, there are no metrics to assess technical proficiency. We aimed to determine if a Quick APC Training Test (QAPCTT) can improve performance and assess proficiency with this modality.Materials and methodsEndoscopy trainees at various levels of training were asked to perform the QAPCTT with an in vivo model before and after an APC curriculum with didactic lectures and additional hands-on experience. As trainees performed the test, endoscopic supervisors recorded the time required to complete each task as well as the number of inadvertent mucosal touchdowns. Each partipant was assigned a technical proficiency score by supervising endoscopists.ResultsFourteen adult gastroenterology fellows participated in the course. 100% of fellows were comfortable with generator settings and APC equipment after the course compared to only 21% (p 
  • Pleiotrophin: Analysis of the endothelialisation potential
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Francesco Copes, Martina Ramella, Luca Fusaro, Diego Mantovani, Mario Cannas, Francesca BoccafoschiAbstractPurposeEndothelialisation of vascular substitutes, in fact, remains one of the most unsolved problems in cardiovascular diseases treatment. Stromal Derived Factor 1 (SDF-1) has been largely investigated as an endothelialisation promoter and Pleiotrophin is a promising alternative. Although it has been known to exert beneficial effects on different cell types, its potential as an inducer of proliferation and migration of endothelial cells was not investigated. Therefore, this work is aimed to compare the effects of Pleiotrophin on proliferation and migration of endothelial cells with respect to SDF-1.Materials/methodsEndothelial cell line EA.hy926 was treated with Pleiotrophin (50 ng/ml) or SDF-1 (50 ng/ml). Cell viability was evaluated by MTT assay and migration assays were performed in Transwell chambers. Wound healing potential was evaluated by scratch wound assay. CXCR4, RPTP β/ζ, PCNA and Rac1 expression was detected by Western Blot.ResultsInterestingly, Pleiotrophin significantly increased the viability of the treated endothelial cells with respects to SDF-1. The migratory ability of the endothelial cells was also improved in the presence of Pleiotrophin with reference to the SDF-1 treatment. Moreover, Western Blot analysis showed how the treatment with Pleiotrophin can induce an increase in the expression of RPTP β/ζ, PCNA and Rac1 compared to SDF-1.ConclusionDue to the significant effects exerted on viability, migration and repair ability of endothelial cells compared to SDF-1, Pleiotrophin can be considered as an interesting molecule to promote re-endothelialisation.
  • Association of 18bp insertion/deletion polymorphism, at −2549 position
           of VEGF gene, with diabetic vascular complications in type 2 diabetes
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Agnieszka Gala-Błądzińska, Joanna Czech, Marcin Braun, Marzena Skrzypa, Krzysztof Gargasz, Artur Mazur, Izabela ZawlikAbstractPurposeDiabetes mellitus type 2 (T2DM) and its vascular complications are a serious world health problem. For this reason it is important to look for new diabetes complication risk factors. The aim of this study was to determine whether 18-bp insertion/deletion (I/D) polymorphism at −2549 position of the vascular endothelial growth factor (VEGF) gene is associated with diabetic vascular complications (DVC).Material and methodsCaucasian subjects (n = 100) with T2DM were recruited for this study. Genotyping of the VEGF gene I/D polymorphism was done by the polymerase chain reaction (PCR) method. The results were correlated with laboratory and clinical data.ResultsIn our population heterozygous of the VEGF gene polymorphism was observed most frequently (57%). DVC were observed in 53 patients. Heterozygous T2DM patients significantly more often suffered from heart failure (HF) and stroke (p = 0.05). Amongst all the DVC, D allele of the VEGF polymorphism had a significantly increased risk of diabetic retinopathy (DR) (OR = 1.31; p = 0.033) irrespective of the duration of diabetes, BMI, the glycemia control expressed by HbA1c, renal function, lipid values or applied treatment. The studied polymorphism did not correlate with coronary heart disease, peripheral vascular disease, cardiovascular death, diabetic kidney disease or applied treatment.ConclusionsThe multivariate logistic regression analysis showed that the D allele in the promoter region of the VEGF gene is an independent risk factor of DR irrespective of other laboratory and clinical variables in T2DM patients. Our study suggests that I/D allele in the studied gene is associated with HF and strokes.
  • Cardiac troponins in chronic kidney disease patients with special emphasis
           on their importance in acute coronary syndrome
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Joanna Szczykowska, Tomasz Hryszko, Beata NaumnikAbstractTroponin measurement is one of crucial assessments facilitating diagnosis of acute coronary syndrome. Patients with chronic kidney disease are decimated by cardiovascular disease. Unfortunately, elevated concentration of serum troponin is commonly faced in clinical practice creating a challenge to rule out acute cardiac ischaemia in this vulnerable population. This review presents current knowlegde on analytical differences in troponin T and I measurements, their prognostic significance and their application in diagnosing acute coronary syndrome in chronic kidney disease patients. It also points out poorly known aspects and suggests directions for future research.
  • Mast cells in mastocytosis and allergy – Important player in metabolic
           and immunological homeostasis
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Joanna Renke, Sabina Kędzierska-Mieszkowska, Magdalena Lange, Bogusław Nedoszytko, Eliza Wasilewska, Anna Liberek, Marcin Renke, Marek Niedoszytko, Jacek Witkowski, Joanna Skórko-Glonek, Barbara LipińskaAbstractThe role of mast cell (MC) activity in pathophysiology is complex and challenging and its clinical effects are difficult to predict. Apart from the known role of MCs in basic immunological processes and allergy, underlined is their importance in bone mineralization and in regulation of autoimmune reactions. Mast cell mediators, especially those released from mast cells in degranulation, but also those released constitutively, are important both in metabolic and immunological processes. Mastocytosis is a heterogeneous group of disorders characterized by accumulation of MC in one or more organs. There are scientific data indicating that mastocytosis patients are at increased risk of osteoporosis in the systemic form of the disease and children with cutaneous mastocytosis have a higher rate of hypogammaglobulinemia. Moreover, the origin of osteoporosis in patients with allergy is no longer considered as linked to steroid therapy only, but to the mast cell mediators’ activity as well. There are indications that osteoporosis symptoms in this group of patients may develop independently of the cumulative steroids’ dose. Thus, the influence of mast cells on metabolic and immunologic processes in allergic patients should be investigated. The assessment of mast cell activity and burden in mastocytosis may be used to guide clinical management of patients with allergy.
  • Combination therapy profoundly improved skin flap survival by modulating
           KATP channels and nitric oxide
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Mahtab Farrokhi, Mehdi Zekriyapanah Gashti, Mahmood Hoormand, Azam Bakhtiarian, Rohalah HabibiAbstractPurposeA potential therapeutic approach on skin flap necrosis is to target parallel pathways involved in necrosis. Azelaic Acid, Minoxidil and Caffeine combination was tried on skin flap survival by their possible interaction with ATP sensitive potassium (KATP) channels and nitric oxide pathway.Material and methodsSprauge-Dawley rats were divided into 8 groups for skin flap surgery. Azelaic acid, minoxidil, caffeine, or their combination were applied topically in different groups. Two additional groups were treated with L-NAME or glibenclamide in addition to the combination therapy. Percentage of flap necrosis was calculated and flap samples were removed to measure tissue malondialdehyde (MDA) and nitric oxide (NO) and expression of inducible nitric oxide synthase (iNOS), Bcl-2 and Bax proteins.ResultsCombination therapy profoundly decreased skin flap necrosis, tissue MDA contents, and expression of the pro-apoptotic protein Bax (p 
  • The concentration-dependent effect of anethole on collagen, MMP-2 and GAG
           in human skin fibroblast cultures
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Edyta Andrulewicz-Botulińska, Iwona Kuźmicz, Jolanta Nazaruk, Joanna Wosek, Anna GalickaAbstractPurposeIn aging skin and some skin disorders, components of skin extracellular matrix (ECM) are disturbed and therefore research to find skin drugs is important. Evaluation of anethole impact on collagen, GAGs and MMP-2 in human skin fibroblasts was the aim of this study.Materials and methodsFor collagen assay the Sircol dye, 5-[3H]proline and real time-PCR were used. MMP-2 activity was detected by zymography. GAG concentration was determined using 1,9-dimethylmethylene blue (DMMB). Cell viability was assayed with MTT.ResultsIn cells treated with 1 and 10 μM anethole, a significant increase in collagen synthesis was demonstrated. In contrast, collagen synthesis was significantly decreased in cells exposed to 100 μM anethole. Similar alterations were found in collagen type I expression. The concentration of collagen secreted into the medium was higher only in cells exposed to 1 μM anethole, while it was lower under the influence of higher compound concentrations. It may be due to the lack of pro-MMP-2 activation at 1 μM and a significant increase in the level of MMP-2 at 10 and 100 μM anethole. GAG concentration was reduced under the influence of 100 μM anethole, whereas anethole at lower concentrations revealed the ability to prevent H2O2-induced GAG increase. No significant cytotoxicity of anethole to fibroblasts was noted.ConclusionsOur findings demonstrate the concentration-dependent action of anethole on the crucial components of ECM in cultured skin fibroblasts, which may be somewhat beneficial and may possibly be developed towards a therapeutic use in some skin disorders.
  • Metabolic pathways of L-arginine and therapeutic consequences in tumors
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Jarosław Szefel, Aleksandra Danielak, Wiesław Janusz KruszewskiAbstractDifference in the metabolism of normal and cancer cells inspires to search for new, more specific and less toxic therapies than those currently used.The development of tumors is conditioned by genetic changes in cancer-transformed cells, immunological tolerance and immunosuppression. At the initial stages of carcinogenesis, the immune system shows anti-tumor activity, however later, cancer disrupts the function of Th1/Th17/Th2 lymphocytes by regulatory T (Treg) cells, tumor-associated macrophages (TAMs), and myeloid-derived suppressor cells (MDSCs) and finally causes immunosuppression.Recently, much attention has been devoted to the influence of l-arginine metabolism disorders on both carcinogenesis and the immune system. l-Arginine is essential for the maturation of the T cell receptor zeta (TCRζ), and its absence deprives T-cells of the ability to interact with tumor antigens. MDSCs deplete l-arginine due to a high expression of arginase 1 (ARG1) and their number increases 4–10 times depending on the type of the cancer.L-Arginine has been shown to be essential for the survival and progression of arginine auxotrophic tumors. However, the progression of arginine non-auxotrophic tumors is independent of exogenous l-arginine, because these tumors have arginine-succinate synthetase (ASS1) activity and are available to produce l-arginine from citrulline.Clinical studies have confirmed the high efficacy of arginine auxotrophic tumors therapy based on the elimination of l-arginine. However, l-arginine supplementation may improve the results of treatment of patients with arginine non-auxotrophic cancer.This review is an attempt to explain the seemingly contradictory results of oncological therapies based on the deprivation or supplementation of l-arginine.
  • The implantation of the scleral-fixated posterior chamber intraocular lens
           with 9/0 polypropylene sutures – Long-term visual outcomes and
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Ewa Wasiluk, Pawel Krasnicki, Diana A. Dmuchowska, Ewa Proniewska-Skrętek, Zofia MariakAbstractPurposeTo analyze long-term visual outcomes and complications of the implantation of a scleralfixated posterior chamber intraocular lens (PC-IOL) in patients with the lack of adequate capsular support, and to verify if the procedure can be performed with 9-0 polypropylene sutures.MethodsThe study was designed as a long-term retrospective analysis. Patients after the implantation of a scleralfixated PC-IOL were evaluated for the best corrected visual acuity (BCVA), intraocular pressure and occurrence of postoperative complications.ResultsThe analysis included 29 eyes from 28 patients. Indications for the IOL implantation included ocular trauma (89.7%) and previous complicated cataract surgery (10.3%). A postoperative improvement of BCVA was observed in 25 eyes (86.2%). Mean follow-up time was 63.9 months (range 50–83 months). During this time, six patients (21.4%) were diagnosed with glaucoma (21.4%), and retinal detachment was found in one eye (3.4%). A total of six suture breakages were recorded in four eyes from four patients (13.8%); one breakage was precipitated by a trauma, and another five, involving three eyes from three patients, were spontaneous. Mean time to the spontaneous suture breakage was 40.8 months.ConclusionsScleral fixation of the PC-IOL provides satisfactory visual outcomes. However, this procedure is associated with a considerable risk of postoperative complications. The incidence of postoperative suture breakage in our series was similar as in previous studies in which the PC-IOL was fixated with 10-0 polypropylene suture. A superiority of 9-0 polypropylene suture needs to be verified in larger series of consecutive patients.
  • Serum levels and gene expression of pentraxin 3 are elevated in COPD
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Michał Poznański, Ewa Brzeziańska-Lasota, Justyna Kiszałkiewicz, Ilona Kurnatowska, Jadwiga Kroczyńska-Bednarek, Anna Pękala-Wojciechowska, Tadeusz Pietras, Adam AntczakAbstractPurposePentraxin 3 (PTX-3) is an acute phase protein that belongs to the pentraxin superfamily. It is synthesized locally at the site of inflammation and its levels are related to the damage of blood vessels. There are only a few studies examining the relationship between PTX-3 and chronic obstructive pulmonary disease (COPD). The aim of this study was to evaluate the serum levels of PTX-3 and relative PTX-3 gene expression in COPD patients and their correlations with cigarette smoking history and lung function.Materials/methodsA total number of 34 participants were enrolled into this study. Only stable patients without comorbidities were recruited. After obtaining written informed consent all planned procedures were performed (pre- and post-bronchodilator spirometry, blood samples for PTX-3 serum levels and PTX-3 gene expression measurements, demographical data, medical history, COPD patients were also asked for CAT and MMRC questionnaires).ResultsPTX-3 serum levels were significantly higher in the COPD group (29.22 (5.47) ng/ml vs. 14.64 (3.64) ng/ml). PTX-3 gene relative quantification (RQ) values were also significantly higher in the COPD group (0.15 (1.33) vs. -2.80 (1.99)). No differences in CRP serum levels were found between the control group and the COPD group.ConclusionsOur study demonstrates that serum levels of PTX-3 and the relative expression values of its gene are elevated in COPD, and can be related to cigarette smoking history.
  • DNA variants in Helicobacter pylori infected patients with chronic
           gastritis, dysplasia and gastric cancer
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Andrzej Hnatyszyn, Marlena Szalata, Marzena Skrzypczak-Zielinska, Karolina Wielgus, Jerzy Stanczyk, Ireneusz Dziuba, Adam Mikstacki, Agnieszka Dobrowolska, Malgorzata Waszak, Piotr Tomasz Hnatyszyn, Ryszard SlomskiAbstractPurposeThe main scope of this study was to evaluate the importance of selected DNA variants for developing inflammation of gastric mucosa and carcinogenesis in gastrointestinal diseases in patients infected with Helicobacter pylori.Patients and methodsPatients subjected to analysis constituted a group of 131 consecutive cases, with control groups consisting of 100 healthy volunteers and 13 dyspeptic patients. Molecular analysis included the following genes: TP53 (c.743 G > A, c.746 G > A, c.749C > T), MSH2 (c.942 + 3A > T), MLH1 (c.2041 G > A), NOD2/CARD15 (c.3016_3017insC, c.802C > T), IL1A (c.-949C > T) and IL1B (c.315C > T). DNA variants were detected using PCR-RFLP, pyrosequencing and sequencing.ResultsMutations of the analyzed genes were observed more frequently in patients with a higher degree of mucosal lesions (50.9%) than in patients with milder mucosal changes (27.6%). Single mutations and polymorphisms did not affect the course of the disease. Our analysis confirms the influence of the NOD2/CARD15 c.802C > T polymorphism on the development of mucosal changes. A correlation of the frequency of the CT genotype of the NOD2/CARD15 c.802C > T polymorphism with the NOD2/CARD15 c.3016_3017insC mutation was observed. The TT genotype frequency in the c.315C > T IL1B gene polymorphism was statistically significantly higher in patients with mucosa changes.ConclusionsAccumulation of molecular abnormalities may increase the susceptibility to inflammatory response of the gastric mucosa in H. pylori-infected patients and play an important role in the development of chronic active gastritis, atrophy, intestinal metaplasia, dysplasia and the intestinal type of gastric cancer. The severity of gastric mucosal damage correlates with the presence of mutations in the gastric mucosa and the age of patients.
  • 16 α-Hydroxyestrone induced adduct generate high affinity
           autoantibodies in SLE
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Wahid Ali KhanAbstractPurposeIncreased 16 α-hydroxyestrone (16 α-OHE1) and autoantibodies against histone H1 (H1) have been well described in systemic lupus erythematosus (SLE), but the combination effects of 16 α-OHE1 and H1 remains unclear. Here, we tried to assess the affinity and binding specificity of SLE autoantibodies against the 16 α-OHE1-H1 adduct. IgG was induced against this adduct and was also used as immunochemical probe for the estimation of 16 α-OHE1 in the serum of SLE patients.Materials and methodsThe affinity and binding specificities of SLE autoantibodies against 16 α-OHE1-H1 were determined by direct binding and inhibition ELISA as well as quantitative precipitation titration in 60 patients and 30 control subjects.ResultsPurified SLE autoantibodies showed greater recognition to 16 α-OHE1-H1 over H1 (p 
  • Beneficial effect of ovocystatin on the cognitive decline in APP/PS1
           transgenic mice
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Bartłomiej Stańczykiewicz, Marta Jakubik-Witkowska, Maria Rutkowska, Antoni Polanowski, Jakub Gburek, Krzysztof Gołąb, Katarzyna Juszczyńska, Tadeusz Trziszka, Joanna RymaszewskaAbstractPurposeCystatin C plays an important role in the course of neurodegenerative diseases and has a beneficial effect through inhibiting cysteine proteases and amyloid-β aggregation. It also induces proliferation and autophagy. Cystatin isolated from chicken egg white, called ovocystatin, has been widely used in the medical and pharmaceutical research due to its structural and biological similarities to human cystatin C. The aim of this study was to assess the effect of administering ovocystatin on the development of dementia-specific cognitive deficits in APP/PS1 transgenic mice.Materials/methodsThe study was conducted on transgenic B6C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax mice. Ovocystatin was administered to four-month-old transgenic (AD) and wild type (NCAR) mice in drinking water for 24 weeks (at a dose of 40 and 4 μg/ mouse). The locomotor activity and cognitive functions were determined using an actimeter and the Morris water maze test, respectively.ResultsThe results of the study indicate that ovocystatin has a beneficial effect on the cognitive functions in APP/PS1 transgenic mice. The strongest effects of ovocystatin were found in the group of AD mice, where ovocystatin was administered in drinking water at a dose of 40 μg/mouse (p 
  • Epilepsy identification based on EEG signal using RQA method
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Iwona Gruszczyńska, Romuald Mosdorf, Piotr Sobaniec, Milena Żochowska-Sobaniec, Marta BorowskaAbstractPurposeEpilepsy is one of the most common neurological diseases and its cause is not unequivocal. Thus, additional methods and searches that may help to diagnose the disease are used in the clinical practice. In this study, we tested the possibility of using the Recurrence Quantification Analysis (RQA) method to identify epilepsy and present the analysis of EEG signals of healthy patients and epileptic patients by the RQA method.Materials/methodsThe recordings of signals belong to 13 patients, which were divided into 2 groups: Group A (5 epileptic patients) and Group B (8 healthy patients). In this study Fp1, Fp2, T3 and T4 electrodes were considered in the analysis using the RQA method.ResultsIt is difficult to explore the dynamics of signals by linear methods. In this study, another way of analyzing the dynamics of signals by the RQA method is presented. The RQA method revealed differences in the dynamics between the epileptic and normal signals, which seemed important in an organoleptic way. It was found that the dynamics of epileptic signals is more periodic than normal signals. To confirm the correctness of the statements issued for the RQA data the Principal Component Analysis mapping was applied. This method showed more clearly the differences in the dynamics of both signals.ConclusionsThe RQA method can be used to identify nonlinear biomedical signals such as EEG signals.
  • Effect of Lactobacillus acidophilus and Porphyromonas gingivalis on
           proliferation and apoptosis of gingival epithelial cells
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Jun-jun Zhao, Long Jiang, Ya-qin Zhu, Xi-ping FengAbstractPurposeThis study aimed to evaluate the possible antagonistic effects of Lactobacillus acidophilus on Porphyromonas gingivalis, and detect inhibition of Lactobacillus acidophilus on Porphyromonas gingivalis when they are co-cultured with human gingival epithelial cells.Materials and methodsHuman gingival epithelial cells were co-cultured with Lactobacillus acidophilus and Porphyromonas gingivalis alone or together. The amount of Porphyromonas gingivalis adhering to or invading the epithelial cells were determined by bacterial counts. The cellular proliferation was assayed by the MTT method. Apoptosis was detected by flow cytometry with apoptosis detection kit.ResultsOn one hand, Lactobacillus acidophilus reduced the inhibitory effect of Porphyromonas gingivalis on the human gingival epithelial cells proliferation in a dose dependent manner. On the other hand, Porphyromonas gingivalis induced significant apoptosis on human gingival epithelial cells, and Lactobacillus acidophilus inhibited this apoptosis-inducing effect of Porphyromonas gingivalis in a dose dependent manner.ConclusionsPorphyromonas gingivalis inhibits the proliferation and induces the apoptosis of human gingival epithelial cells. Lactobacillus acidophilus could attenuate this effect in a dose-dependent manner, and it thus reduces the destruction from pathogens. Lactobacillus acidophilus could be an effective candidate for probiotic therapy in periodontal diseases.
  • Association between gastric myoelectric activity disturbances and
           dyspeptic symptoms in gastrointestinal cancer patients
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Aneta L. Zygulska, Agata Furgala, Krzysztof Krzemieniecki, Beata Wlodarczyk, Piotr ThorAbstractPurposeDyspeptic symptoms present a severe problem in gastrointestinal (GI) cancer patients. The aim of the study was to analyze an association between gastric myoelectric activity changes and dyspeptic symptoms in gastrointestinal cancer patients.Material and MethodsThe study included 80 patients (37 men and 43 women, mean age 61.2 ± 7.8 years) diagnosed with GI tract malignancies: colon (group A), rectal (group B) and gastric cancers (group C). Gastric myoelectric activity in a preprandial and postprandial state was determined by means of a 4-channel electrogastrography. Autonomic nervous system was studied based on heart rate variability analysis. The results were compared with the data from healthy asymptomatic controls.ResultsIn a fasted state, GI cancer patients presented with lesser percentages of normogastria time (A:44.23 vs. B:46.5 vs. C:47.10 vs. Control:78.2%) and average percentage slow wave coupling (ACSWC) (A:47.1 vs. B:50.8 vs. C:47.2 vs. Control:74.9%), and with higher values of dominant power (A:12.8 vs. B:11.7 vs. C:12.3 vs. Control:10.9) than the controls. Patients did not show an improvement in the percentage of normogastria time, dominant power, dominant frequency and ACSWC in response to food. The severity of dyspeptic symptoms correlated with the values of electrogastrography parameters. Patients showed lower values of heart rate variability parameters than the healthy controls, that indicate abnormal autonomic nervous system activity.ConclusionGI cancers affect the gastric myoelectric activity, decreasing normogastria and slow wave coupling. These patients do not show adequate gastric motility response to food. Impaired gastric electric motility may result from cancer-induced autonomic disturbances.
  • Fluid therapy in non-septic, refractory acute decompensated heart failure
           patients – The cautious role of central venous pressure
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Malgorzata Chlabicz, Remigiusz Kazimierczyk, Paulina Lopatowska, Monika Gil-Klimek, Bartosz Kudlinski, Marcin Ligowski, Bozena Sobkowicz, Marek Gierlotka, Karol Kaminski, Agnieszka TycinskaAbstractPurposeFluid therapy in congestive acute decompensated heart failure (ADHF) patients might be inappropriate and worsening the prognosis. The aim of our study was to analyze the effect of fluid administration on mortality in non-septic, ADHF patients with reduced ejection fraction.Material and methodsWe analyzed 41 ADHF consecutive ‘cold-wet’ patients (mean age 69.3 ± 14.9 years, 27 men, LVEF 22.8 ± 11.1%, lactates 2.2 ± 1.6 mmol/L) without sepsis. At admission central venous pressure (CVP) was measured (17.6 ± 7.2 cm H2O), and ultrasound examination of inferior vena cava (IVC) was performed (IVC min. 18.6 ± 7.3 mm and IVC max. 24.6 ± 4.3 mm). Moreover, the groups were compared (survivors vs. non-survivors as well as 1st and 4th quartile of CVP).ResultsAltogether 17 (41%) patients died: 16 (39%) during a mean of 11.2 ± 7.8 days of hospitalization and 1 during a 30-day follow up. Patients in the lowest CVP quartile (24 cm H2O), P = 0.012. Higher intravenous fluid volumes within the first 24 h were infused in patients in the lowest CVP quartile as compared to the highest CVP quartile (1791.7 ± 1357.8 mL vs. 754.5 ± 631.4 mL, P = 0.046). Moreover, more fluids were infused in a group of patients who died during a hospital stay and at 30-day follow up (1362.8 ± 752.7 mL vs. 722.7 ± 1046.5 mL, P = 0.004; 1348.8 ± 731.0 mL vs. 703.6 ± 1068.4 mL, P = 0.002, respectively).ConclusionsCVP-guided intravenous fluid therapy is a common practice which in high risk ADHF ‘cold-wet’ patients might be harmful and should rather be avoided. Lower CVP seems to be related with worse prognosis.
  • Hypertension prevalence in early breast cancer patients undergoing primary
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Klaudia Kozłowska, Leszek Kozłowski, Jolanta MałyszkoAbstractPurposeTreatment with chemotherapy and targeted drugs may results in elevated risk of cardiac and renal toxicity as well as hypertension. However, data on prevalence of chronic kidney disease and hypertension in subjects with early breast cancer undergoing primary surgery are very limited.Patients and methodsThe study aimed to assess the prevalence of chronic kidney disease and hypertension (evaluated as a preoperative assessment and defined according to ESC/ESH guidelines) in a cohort of 100 consecutive female patients with early breast cancer treated with primary surgery with curative intent.ResultsPatients with breast cancer were 53 ± 14 years of age, with serum creatinine of 0.68 ± 0.14 mg/dl and estimated glomerular filtration rate by chronic kidney disease-epidemiological collaboration formula of 99 ± 18 mL/min/1.72 m2. Hypertension was present in 37%, but in the elderly patients (over 65 years) the prevalence was 74%. Hypertensive females had worse kidney function as reflected by higher serum creatinine and lower estimated glomerular filtration rate, higher body mass index and fibrinogen, which reflects general inflammatory state. When we divided the patients according to age (≤ vs>65 years) and the presence of hypertension, the elderly hypertensive females had significantly worse kidney function, higher fibrinogen and fasting glucose.ConclusionsThe prevalence of hypertension in patients with breast cancer raises with age, and presence of comorbidities, including chronic kidney disease. Hypertension should be treated promptly to prevent cardiovascular complications during oncological therapy.
  • Activity of lysosomal exoglycosidases in the urine of healthy normotensive
           and pre-hypertensive children
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Beata Zalewska-Szajda, Katarzyna Taranta-Janusz, Sylwia Chojnowska, Napoleon Waszkiewicz, Krzysztof Zwierz, Anna WasilewskaAbstractPurposeWe investigated the relationship between blood pressure (BP) and the activity of lysosomal exoglycosidases: N-acetyl-β-hexosaminidase (HEX), its isoenzymes A (HEX A) and B (HEX B), α-fucosidase (FUC), β-galactosidase (GAL), β-glucuronidase (GLU) and α-mannosidase (MAN) in pre-hypertensive (high normal blood pressure - HNBP) and normal blood pressure (NBP) children.Material and methodsThe study was carried out with urine samples collected from 176 children, aged 6–17.9 years, divided into 2 groups: 42 HNBP and 134 NBP subjects. The children were stratified depending on systolic and diastolic BP (SBP; DBP): HNBP (SBP and/or DBP greater than or equal to the 90th percentile, but less than the 95th percentile) for sex, age, and height; and NBP (SBP and DBP less than the 90th centile). The activities of lysosomal exoglycosidases were determined by the colorimetric method, and expressed in pKat/mL and pKat/μgCr.ResultsThe activity of urinary HEX A in HNBP group was significantly higher than in NBP (p 
  • Long-term administration of fatty acid amide hydrolase inhibitor (URB597)
           to rats with spontaneous hypertension disturbs liver redox balance and
           phospholipid metabolism
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Michał Biernacki, Ewa Ambrożewicz, Agnieszka Gęgotek, Marek Toczek, Elżbieta SkrzydlewskaAbstractPurposeThe effect of chronic administration of [3-(3-carbamoylphenyl)phenyl] N-cyclohexylcarbamate (URB597), inhibitor of fatty acid amide hydrolase (FAAH) that hydrolyzes anandamide, on cross-talk between endocannabinoid system, oxidative status and pro-inflammatory factors in the liver of spontaneously hypertensive rats (SHRs) was investigated.Materials/methodsExperiments were conducted using SHRs and normotensive control Wistar-Kyoto rats treated by intraperitoneal injection with URB597 for 14 days. The biochemical parameters were assayed in the rat’s livers.ResultsIn the liver of SHRs an increase in endocannabinoids level, the activity of enzymes degrading them and expression of the cannabinoid receptor type 2 (CB2) receptor as well as a decrease in the expression of the CB1 and vanilloid 1 receptor (TRPV1) were shown. These changes were related to inflammatory conditions as well as oxidative stress resulting from increased reactive oxygen species (ROS) generation due to enhanced activity of enzymes generating ROS accompanied by decrease in the effectiveness of transcription activity of nuclear factor erythroid 2 and the activity of antioxidant enzymes, as well as level of glutathione and vitamins. Chronic administration of URB597 to SHRs caused a decrease in FAAH activity and an increase in anandamide and N-arachidonoyl-dopamine level as well as a decrease in CB2 and an increase in TRPV1 receptor expression. The levels/activities of pro- and antioxidant and inflammatory factors tended to normalize, but phospholipid peroxidation and DNA modifications were increased.ConclusionIn conclusion, long-term chronic administration of URB597 to SHRs by altering interactions between endocannabinoid and redox systems enhances some liver metabolic disturbances observed in hypertension.
  • Diagnostic power of VEGF, MMP-9 and TIMP-1 in patients with breast cancer.
           A multivariate statistical analysis with ROC curve
    • Abstract: Publication date: March 2019Source: Advances in Medical Sciences, Volume 64, Issue 1Author(s): Monika Zajkowska, Ewa Gacuta, Sylwia Kozłowska, Emilia Lubowicka, Edyta K. Głażewska, Lech Chrostek, Maciej Szmitkowski, Przemysław Pawłowski, Monika Zbucka-Krętowska, Sławomir ŁawickiAbstractPurposeVascular endothelial growth factor is an important factor in promoting angiogenesis in malignant processes, matrix metalloproteinase-9 in the degradation of extracellular matrix, which enhances metastasis, and tissue inhibitor of metalloproteinase-1 is its inhibitor. The aim of this study was to investigate the diagnostic power of these parameters in comparison to CA15-3 in breast cancer patients and in relation to the control group.Materials/methodsThe study included 120 breast cancer patients, 60 patients with benign breast tumors and 60 healthy women. Plasma levels of tested parameters were determined by enzyme-linked immunosorbent assay, CA15-3 by chemiluminescent microparticle immuno assay.ResultsTissue inhibitor of metalloproteinase-1 showed the highest value of sensitivity in breast cancer group (86.25%) and, more importantly, highest value in breast cancer stage I (85%). Vascular endothelial growth factor also showed high sensitivity (stage I and II–75%, III–85%, IV–70% and 76.25% in total breast cancer group) and the highest specificity (85%) from all tested parameters. It was also the only parameter which had statistically significant area under curve in all stages. In the total breast cancer group all tested parameters showed statistically significant area under curve, but the maximum range was obtained for combination: ‘vascular endothelial growth factor + CA15-3′. Vascular endothelial growth factor seems to be the best candidate for diagnosing breast cancer stage I and for differentiating between breast cancer and non-carcinoma cases.ConclusionsThe combined analysis of tested parameters and CA15-3 resulted in an increase in sensitivity and area under curve values, which provides hope for developing new panel of biomarkers that may be used in diagnosing breast cancer in the future.
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