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Showing 1 - 200 of 3562 Journals sorted alphabetically
16 de Abril     Open Access  
3D Printing in Medicine     Open Access   (Followers: 1)
AADE in Practice     Hybrid Journal   (Followers: 5)
ABCS Health Sciences     Open Access   (Followers: 3)
Abia State University Medical Students' Association Journal     Full-text available via subscription  
ACIMED     Open Access   (Followers: 1)
ACS Medicinal Chemistry Letters     Hybrid Journal   (Followers: 41)
Acta Bio Medica     Full-text available via subscription   (Followers: 2)
Acta Bioethica     Open Access  
Acta Bioquimica Clinica Latinoamericana     Open Access   (Followers: 1)
Acta Facultatis Medicae Naissensis     Open Access  
Acta Informatica Medica     Open Access   (Followers: 1)
Acta Medica (Hradec Králové)     Open Access  
Acta Medica Bulgarica     Open Access  
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Acta Medica International     Open Access  
Acta medica Lituanica     Open Access  
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Acta Medica Nagasakiensia     Open Access  
Acta Medica Peruana     Open Access   (Followers: 2)
Acta Médica Portuguesa     Open Access  
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Acta Scientiarum. Health Sciences     Open Access   (Followers: 1)
Acupuncture & Electro-Therapeutics Research     Full-text available via subscription   (Followers: 4)
Acupuncture and Natural Medicine     Open Access  
Addiction Science & Clinical Practice     Open Access   (Followers: 6)
Addictive Behaviors Reports     Open Access   (Followers: 8)
Adıyaman Üniversitesi Sağlık Bilimleri Dergisi / Health Sciences Journal of Adıyaman University     Open Access  
Adnan Menderes Üniversitesi Sağlık Bilimleri Fakültesi Dergisi     Open Access  
Advanced Biomedical Research     Open Access  
Advanced Health Care Technologies     Open Access   (Followers: 4)
Advanced Science, Engineering and Medicine     Partially Free   (Followers: 7)
Advances in Bioscience and Clinical Medicine     Open Access   (Followers: 5)
Advances in Clinical Chemistry     Full-text available via subscription   (Followers: 28)
Advances in Life Course Research     Hybrid Journal   (Followers: 8)
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Advances in Parkinson's Disease     Open Access  
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Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 20)
Advances in Regenerative Medicine     Open Access   (Followers: 2)
Advances in Skeletal Muscle Function Assessment     Open Access  
Advances in Therapy     Hybrid Journal   (Followers: 5)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 13)
Advances in Virus Research     Full-text available via subscription   (Followers: 5)
Advances in Wound Care     Hybrid Journal   (Followers: 10)
Aerospace Medicine and Human Performance     Full-text available via subscription   (Followers: 11)
African Health Sciences     Open Access   (Followers: 2)
African Journal of Biomedical Research     Open Access  
African Journal of Clinical and Experimental Microbiology     Open Access   (Followers: 1)
African Journal of Laboratory Medicine     Open Access   (Followers: 2)
African Journal of Medical and Health Sciences     Open Access   (Followers: 2)
African Journal of Trauma     Open Access   (Followers: 1)
Afrimedic Journal     Open Access   (Followers: 2)
Aggiornamenti CIO     Hybrid Journal   (Followers: 1)
AIDS Research and Human Retroviruses     Hybrid Journal   (Followers: 8)
AJOB Primary Research     Partially Free   (Followers: 3)
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Aktuelle Ernährungsmedizin     Hybrid Journal   (Followers: 4)
Al-Azhar Assiut Medical Journal     Open Access  
Alexandria Journal of Medicine     Open Access   (Followers: 1)
Allgemeine Homöopathische Zeitung     Hybrid Journal   (Followers: 2)
Alpha Omegan     Full-text available via subscription  
ALTEX : Alternatives to Animal Experimentation     Open Access   (Followers: 3)
American Journal of Biomedical Engineering     Open Access   (Followers: 11)
American Journal of Biomedical Research     Open Access   (Followers: 2)
American Journal of Biomedicine     Full-text available via subscription   (Followers: 7)
American Journal of Chinese Medicine, The     Hybrid Journal   (Followers: 4)
American Journal of Clinical Medicine Research     Open Access   (Followers: 7)
American Journal of Family Therapy     Hybrid Journal   (Followers: 11)
American Journal of Law & Medicine     Full-text available via subscription   (Followers: 11)
American Journal of Lifestyle Medicine     Hybrid Journal   (Followers: 5)
American Journal of Managed Care     Full-text available via subscription   (Followers: 11)
American Journal of Medical Case Reports     Open Access   (Followers: 1)
American Journal of Medical Sciences and Medicine     Open Access   (Followers: 4)
American Journal of Medicine     Hybrid Journal   (Followers: 45)
American Journal of Medicine and Medical Sciences     Open Access   (Followers: 1)
American Journal of Medicine Studies     Open Access   (Followers: 1)
American Journal of Medicine Supplements     Full-text available via subscription   (Followers: 3)
American Journal of the Medical Sciences     Hybrid Journal   (Followers: 12)
American Journal on Addictions     Hybrid Journal   (Followers: 8)
American medical news     Free   (Followers: 3)
American Medical Writers Association Journal     Full-text available via subscription   (Followers: 5)
Amyloid: The Journal of Protein Folding Disorders     Hybrid Journal   (Followers: 5)
Anales de la Facultad de Medicina     Open Access  
Anales de la Facultad de Medicina, Universidad de la República, Uruguay     Open Access  
Anales del Sistema Sanitario de Navarra     Open Access   (Followers: 1)
Analgesia & Resuscitation : Current Research     Hybrid Journal   (Followers: 5)
Anatolian Clinic the Journal of Medical Sciences     Open Access  
Anatomical Science International     Hybrid Journal   (Followers: 2)
Anatomical Sciences Education     Hybrid Journal   (Followers: 1)
Anatomy     Open Access  
Anatomy Research International     Open Access   (Followers: 2)
Angewandte Schmerztherapie und Palliativmedizin     Hybrid Journal  
Angiogenesis     Hybrid Journal   (Followers: 3)
Ankara Medical Journal     Open Access   (Followers: 2)
Ankara Üniversitesi Tıp Fakültesi Mecmuası     Open Access  
Annales de Pathologie     Full-text available via subscription  
Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale     Full-text available via subscription   (Followers: 3)
Annals of African Medicine     Open Access   (Followers: 1)
Annals of Anatomy - Anatomischer Anzeiger     Hybrid Journal   (Followers: 2)
Annals of Bioanthropology     Open Access   (Followers: 4)
Annals of Biomedical Engineering     Hybrid Journal   (Followers: 18)
Annals of Biomedical Sciences     Full-text available via subscription   (Followers: 3)
Annals of Clinical Microbiology and Antimicrobials     Open Access   (Followers: 10)
Annals of Family Medicine     Open Access   (Followers: 12)
Annals of Ibadan Postgraduate Medicine     Open Access  
Annals of Medical and Health Sciences Research     Open Access   (Followers: 7)
Annals of Medicine     Hybrid Journal   (Followers: 12)
Annals of Medicine and Surgery     Open Access   (Followers: 7)
Annals of Microbiology     Hybrid Journal   (Followers: 11)
Annals of Nigerian Medicine     Open Access   (Followers: 1)
Annals of Saudi Medicine     Open Access  
Annals of the New York Academy of Sciences     Hybrid Journal   (Followers: 5)
Annals of The Royal College of Surgeons of England     Full-text available via subscription   (Followers: 3)
Annual Reports in Medicinal Chemistry     Full-text available via subscription   (Followers: 7)
Annual Reports on NMR Spectroscopy     Full-text available via subscription   (Followers: 4)
Annual Review of Medicine     Full-text available via subscription   (Followers: 16)
Anthropological Review     Open Access   (Followers: 23)
Anthropologie et santé     Open Access   (Followers: 5)
Antibiotics     Open Access   (Followers: 9)
Antibodies     Open Access   (Followers: 2)
Antibody Technology Journal     Open Access   (Followers: 1)
Anuradhapura Medical Journal     Open Access  
Anwer Khan Modern Medical College Journal     Open Access   (Followers: 2)
Apmis     Hybrid Journal   (Followers: 1)
Apparence(s)     Open Access   (Followers: 1)
Applied Clinical Research, Clinical Trials and Regulatory Affairs     Hybrid Journal  
Applied Medical Informatics     Open Access   (Followers: 10)
Arab Journal of Nephrology and Transplantation     Open Access   (Followers: 1)
Arak Medical University Journal     Open Access  
Archive of Clinical Medicine     Open Access   (Followers: 1)
Archive of Community Health     Open Access   (Followers: 1)
Archives Medical Review Journal / Arşiv Kaynak Tarama Dergisi     Open Access  
Archives of Medical and Biomedical Research     Open Access   (Followers: 3)
Archives of Medical Laboratory Sciences     Open Access   (Followers: 1)
Archives of Medicine and Health Sciences     Open Access   (Followers: 3)
Archives of Medicine and Surgery     Open Access  
Archives of Trauma Research     Open Access   (Followers: 3)
Archivos de Medicina (Manizales)     Open Access  
ArgoSpine News & Journal     Hybrid Journal  
Arquivos Brasileiros de Oftalmologia     Open Access  
Arquivos de Ciências da Saúde     Open Access  
Arquivos de Medicina     Open Access  
Ars Medica : Revista de Ciencias Médicas     Open Access  
ARS Medica Tomitana     Open Access   (Followers: 1)
Art Therapy: Journal of the American Art Therapy Association     Full-text available via subscription   (Followers: 12)
Arterial Hypertension     Open Access   (Followers: 1)
Artificial Intelligence in Medicine     Hybrid Journal   (Followers: 13)
Artificial Organs     Hybrid Journal   (Followers: 1)
ASHA Leader     Open Access  
Asia Pacific Family Medicine     Open Access   (Followers: 1)
Asia Pacific Journal of Clinical Nutrition     Full-text available via subscription   (Followers: 10)
Asia Pacific Journal of Clinical Trials : Nervous System Diseases     Open Access  
Asian Bioethics Review     Full-text available via subscription   (Followers: 3)
Asian Biomedicine     Open Access   (Followers: 2)
Asian Journal of Cell Biology     Open Access   (Followers: 5)
Asian Journal of Health     Open Access   (Followers: 3)
Asian Journal of Medical and Biological Research     Open Access   (Followers: 3)
Asian Journal of Medical and Pharmaceutical Researches     Open Access   (Followers: 1)
Asian Journal of Medical Sciences     Open Access   (Followers: 2)
Asian Journal of Scientific Research     Open Access   (Followers: 3)
Asian Journal of Transfusion Science     Open Access   (Followers: 1)
Asian Medicine     Hybrid Journal   (Followers: 5)
Asian Pacific Journal of Cancer Prevention     Open Access  
ASPIRATOR : Journal of Vector-borne Disease Studies     Open Access  
Astrocyte     Open Access  
Atención Familiar     Open Access  
Atención Primaria     Open Access   (Followers: 1)
Atti della Accademia Peloritana dei Pericolanti - Classe di Scienze Medico-Biologiche     Open Access  
Audiology - Communication Research     Open Access   (Followers: 9)
Auris Nasus Larynx     Full-text available via subscription  
Australian Coeliac     Full-text available via subscription   (Followers: 1)
Australian Family Physician     Full-text available via subscription   (Followers: 3)
Australian Journal of Medical Science     Full-text available via subscription   (Followers: 1)
Autopsy and Case Reports     Open Access  
Avicenna     Open Access   (Followers: 3)
Avicenna Journal of Clinical Medicine     Open Access  
Avicenna Journal of Medicine     Open Access   (Followers: 1)
Bangabandhu Sheikh Mujib Medical University Journal     Open Access   (Followers: 1)
Bangladesh Journal of Anatomy     Open Access   (Followers: 1)
Bangladesh Journal of Bioethics     Open Access  
Bangladesh Journal of Medical Biochemistry     Open Access   (Followers: 3)
Bangladesh Journal of Medical Education     Open Access   (Followers: 2)
Bangladesh Journal of Medical Microbiology     Open Access   (Followers: 3)
Bangladesh Journal of Medical Physics     Open Access  
Bangladesh Journal of Medical Science     Open Access  
Bangladesh Journal of Medicine     Open Access   (Followers: 1)
Bangladesh Journal of Physiology and Pharmacology     Open Access  
Bangladesh Journal of Scientific Research     Open Access   (Followers: 1)

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Journal Cover
Advances in Medical Sciences
Journal Prestige (SJR): 0.694
Citation Impact (citeScore): 2
Number of Followers: 6  
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1896-1126 - ISSN (Online) 1898-4002
Published by Elsevier Homepage  [3159 journals]
  • The added value of magnifying endoscopy in diagnosing patients with
           certain gastroesophageal reflux disease
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Justyna Wasielica-Berger, Andrzej Kemona, Joanna Kiśluk, Agnieszka Świdnicka-Siergiejko, Paweł Rogalski, Adam Chwieśko, Maja Kostrzewska, Andrzej DąbrowskiAbstractPurposeIn most cases gastroesophageal reflux disease proceeds without macroscopic erosions in the esophagus. We aimed to clarify if abnormalities detectable in magnifying endoscopy may offer additional diagnostic criteria for gastroesophageal reflux disease and to what histopathologic structures do they correspond.Patients/methodsEsophageal mucosa above and below Z-line was evaluated under x115 magnification in 67 gastroesophageal reflux disease patients (11 with erosive reflux disease, 28 with Barrett’s esophagus, 28 with nonerosive reflux disease) and in 12 patients without gastroesophageal reflux disease (negative control group). Features characteristic of gastroesophageal reflux disease were specified by comparing erosive reflux disease and Barrett’s esophagus patients with negative control group. Afterwards the presence of identified features were evaluated in nonerosive reflux disease group. Interobserver agreement in the recognition of the proposed criteria was rated. Biopsies collected from the mucosa above Z-line were evaluated histologically after hematoxylin and eosin staining.ResultsEndoscopic lesions characteristic of gastroesophageal reflux disease were: microerosions, abnormal intrapapillary capillary loops, obscured palisade vessels, white points, big triangular indentations of Z-line and villous mucosa below Z-line. The presence of two or more of the above features indicated gastroesophageal reflux disease with 97% sensitivity and 75% specificity. Substantial interobserver agreement was achieved in evaluation of obscured palisade vessels, abnormal intrapapillary capillary loops and white points. Endoscopic lesions were correlated to histology. Lesions identified with magnifying endoscopy were helpful in discerning between negative control group and nonerosive reflux disease patients.ConclusionsMagnifying endoscopy reveals abnormalities that can be used as additional endoscopic diagnostic criteria of gastroesophageal reflux disease.
  • Effect of maternal weight during pregnancy on offspring muscle strength
           response to resistance training in late adulthood
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Niko S. Wasenius, Mika Simonen, Liisa Penttinen, Minna K. Salonen, Samuel Sandboge, Johan G. ErikssonAbstractPurposeMaternal obesity can unfavorably influence offspring body composition, muscle strength, and possibly muscle’s adaptability to training, but the human studies are scarce. Therefore, we aimed to investigate the effect of maternal obesity on offspring muscle strength responses to resistance training intervention in elderly frail women.Materials/methodsRecruited participants were elderly frail women offspring of lean/normal weight mothers (n = 19, mean body mass index (BMI): 22.8 kg/m2, range: 19.9–24.5) or overweight/obese mothers (n = 16, mean BMI: 29.7 kg/m2, range: 28.2–34.2). Information on maternal BMI immediately prior to delivery was collected from the birth registers. All women participated in a 4-month supervised progressive resistance training intervention three times a week for 60 min. Predicted 1-RM of abdominal crunch, hip abduction, leg curl, leg press, seated row, and total strength were measured at baseline and after each month of training.ResultsAccording to rANOVA, strength increased significantly in both groups (p for time 0.072). On average, muscle strength of the women offspring of overweight/obese mothers tended to be lower than in women offspring of lean/normal weight mothers, but the only significant difference was found in leg curl (p = 0.006). No significant differences between the groups were found in relative strength changes from baseline to 4-months.ConclusionsMuscle strength response to supervised resistance training is not modulated by maternal adiposity in late pregnancy in elderly frail female offspring.
  • Topical folinic acid enhances wound healing in rat model
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Nilay Duman, Reşat Duman, Murat Tosun, Murat Akıcı, Engin Göksel, Barış Gökçe, Oğuzhan AlagözAbstractPurposeFolic acid is an essential vitamin participating in DNA synthesis and repair. Recently folic acid has been shown to stimulate DNA-repair capacity in dermal fibroblasts in response to injury. Thus, the present study aimed to investigate the effects of topical folinic acid, a 5-formyl derivative of tetrahydrofolic acid, on wound healing using rat wound model.Materials and methodsA rat wound model was established, and the wound healing was evaluated by macroscopic and histological analyses among vehicle control, 2.5% folinic acid, 1% folinic acid, and dexpanthenol treatment groups. While an image-analysis program was used to evaluate macroscopic wound closure, connective tissue properties, mast cell numbers, and the expressions of matrix metalloproteinase 1 (MMP-1) and 9 (MMP-9) were evaluated by microscopy.ResultsThe 2.5% folinic acid-treated group exhibited enhanced wound healing by increased reepithelialization, neo-vessel formation, inflammatory cell migration, collagen deposition and progressive mast cell increase. Furthermore, 2.5% folinic acid induced higher expressions of MMP-1 and MMP-9.ConclusionsFolinic acid enhances both macroscopic and microscopic wound healing in rat wound model.
  • Vitamin E status and its determinants in patients with cystic fibrosis
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Ewa Sapiejka, Patrycja Krzyżanowska-Jankowska, Ewa Wenska-Chyży, Mariusz Szczepanik, Dariusz Walkowiak, Szczepan Cofta, Andrzej Pogorzelski, Wojciech Skorupa, Jarosław WalkowiakAbstractPurposeThe risk of vitamin E deficiency is of primary concern in cystic fibrosis patients. However, early diagnosis and routine vitamin E supplementation can lead to its normal or even high levels. In the present study, we assessed vitamin E status in a large group of cystic fibrosis patients. Moreover, we also aimed to establish determinants of its body resources in cystic fibrosis patients.Material and methodsThe study group comprised 211 cystic fibrosis patients aged from 1 month to 48 years. In all of them serum α-tocopherol concentration was analyzed using high-performance liquid chromatography.ResultsMedian vitamin E concentration was 9.9 μg/ml (1st–3rd quartile: 7.5–13.5). Vitamin E deficiency was found in 17 (8.0%) and high levels were documented in 24 (11.4%) participants. Patients with and without vitamin E deficiency did not differ significantly with respect to age, standardized body weight and height, FEV1, albumin concentration and vitamin E supplementation dose. However, vitamin E deficiency appeared more frequently in participants without vitamin E supplementation. Moreover, in multiple linear regression analysis pancreatic insufficiency, severe CFTR gene mutation and vitamin E dose, were potentially defined as determinants of vitamin E concentration.ConclusionsVitamin E deficiency in cystic fibrosis patients is rather rare nowadays. Excessive vitamin E levels seem to be more frequent. Vitamin E status wasn’t documented to be strictly related to clinical determinants. Beyond vitamin E supplementation, exocrine pancreatic function and CFTR gene mutations may have had an impact on the vitamin E body resources in cystic fibrosis patients.
  • Cardiac and autonomic function in patients with Crohn’s disease
           during remission
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Monika Zawadka-Kunikowska, Joanna Słomko, Maria Kłopocka, Ariel Liebert, Małgorzata Tafil-Klawe, Jacek J. Klawe, Julia L. Newton, Paweł ZalewskiAbstractPurposeThe aim of the study was to assess cardiac and autonomic function in patients with Crohn’s disease and explore their relation to disease duration using cardiovascular reflex tests.Materials and methodsCardiovascular parameters, baroreflex sensitivity, spectral-indices of short-term heart rate variability and blood pressure variability were compared between patients with Crohn’s disease in remission (n = 30) and a control group (n = 29). Cardiac autonomic function was assessed during response to standing (tilt) and deep breathing test (expiration/inspiration ratio-E/I). Aortic pulse wave velocity, aortic augmentation index and central systolic blood pressure were measured oscillometrically.ResultsAt rest, Crohn’s disease patients had significantly higher systolic (p = 0.03) and diastolic (p = 0.03) blood pressure, total peripheral resistance index (p = 0.003), sympathetic-parasympathetic ratio (p = 0.033) and lower baroreceptor effectiveness (p = 0.047), myocardial variables (stroke index; p = 0.03, cardiac index; p = 0.025, Heather index; p = 0.039, left ventricular ejection time; p = 0.038), as compared to controls. Orthostatic response to the tilt test in the Crohn’s disease group and the control group was similar, no intergroup differences were observed for E/I ratio and autonomic parameters. In Crohn’s disease patients, disease duration was negatively associated with baroreflex sensitivity and positively correlated with normalised high frequency heart rate variability, sympathetic-parasympathetic ratio at rest and post-tilt changes in Δsystolic blood pressure, p 
  • Cardiovascular comorbidities in a United States patient population with
           hemophilia A: A comprehensive chart review
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Thomas J. Humphries, Brittny Rule, Augustina Ogbonnaya, Michael Eaddy, Orsolya Lunacsek, Lois Lamerato, Jennifer PocoskiAbstractPurposePrevious retrospective claims database analyses reported increased prevalence and earlier onset of cardiovascular comorbidities in patients with versus without hemophilia A. A comprehensive chart review was designed to further investigate previous findings.MethodsThis retrospective chart review study was conducted at Henry Ford Health System (Detroit, MI, USA). Baseline demographics, bleeding events, treatment parameters, coexisting diseases, hemophilia-associated events, Charlson Comorbidity Index score, and prevalence of 12 cardiovascular risk factors and associated diseases were compared between hemophilia A and control cohorts. P values from a chi-square test for categorical variables and a t test for continuous variables were calculated. Because of small sample sizes (N = 0–90, most
  • Plasma concentration of Retinol Binding Protein 4 (RBP4) in relation to
           nutritional status and kidney function in older population of PolSenior
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Piotr Kocełak, Aleksander Owczarek, Maria Bożentowicz-Wikarek, Aniceta Brzozowska, Małgorzata Mossakowska, Tomasz Grodzicki, Andrzej Więcek, Jerzy Chudek, Magdalena Olszanecka-GlinianowiczAbstractPurposeThe aim of the study was to assess plasma RBP4 concentration in elderly subjects in relation to nutritional status and kidney function in the population of the PolSenior Study.Material and methodsWe assessed RBP4, glucose, insulin, albumin, lipid profile, C-reactive protein, (hsCRP) and creatinine concentrations in 2614 PolSenior Study participants (1235 women and 1379 men). The study group was divided based on BMI and HOMA-IR values, and the occurrence of diabetes.ResultsPlasma RBP4 concentration was similar in normal weight, overweight, and obese subgroups, both in women (40.4 vs 40.8 vs 41.8 ng/ml, respectively), and men (41.2 vs 40.3 vs 42.9 ng/ml, respectively). Similar values were found in subjects with HOMA-IR
  • HLA-G and anti-HCV in patients on the waiting list for kidney
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Linda Sommese, Rossella Paolillo, Francesco Cacciatore, Vincenzo Grimaldi, Chiara Sabia, Antonella Esposito, Antonio Sorriento, Carmela Iannone, Nicolò Rupealta, Gerardo Sarno, Michele Santangelo, Paride De Rosa, Gianfranco Nicoletti, Claudio NapoliAbstractPurposeHuman leukocyte antigen (HLA)-G is a non-classic major histocompatibility complex HLA class I molecule. HLA-G may have tolerogenic properties which are linked to epigenetic-sensitive pathways. There is a correlation of sHLA-G levels and graft acceptance in transplantation studies. There are previous data on correlation of sHLA-G with graft rejection as well as with viral infections such as hepatitis C virus (HCV) in kidney transplanted patients. Here, we report the sHLA-G expression in patients on the waiting list for kidney transplantation, with and without anti-HCV compared to a control group.MethodsSerum of 67 patients on the waiting list for kidney transplantation (n = 43 with anti-HCV and n = 24 without anti-HCV) was analyzed. Among these patients, n = 39 were on the waiting list for the first transplantation, while n = 28 were patients who returned in the list. The control group included n = 23 blood donors with anti-HCV (n = 13) and without anti-HCV (n = 10).ResultsThe expression of sHLA-G was significantly lower in the control group (39.6 ± 34.1 U/ml) compared to both - patients on the waiting list for the first transplantation (62.5 ± 42.4 U/ml, p=0.031) and patients who returned in the list (76.7 ± 53.9 U/ml, p=0.006). No significant differences were observed in all anti-HCV positive groups. A positive linear correlation between sHLA-G and TNF-α, and patient age was observed.ConclusionsSerum sHLA-G values were significantly increased in both - patients on the waiting list for the first transplantation and patients who returned in the list, as compared to control group. Our findings confirm the key tolerogenic role of sHLA-G levels as epigenetic-related marker for measuring the state of kidney allograft acceptance.
  • l-fucosidase,+β-d-galactosidase+and+β-d-glucuronidase+determination+in+serum+from+hemolyzed+blood&rft.title=Advances+in+Medical+Sciences&rft.issn=1896-1126&">Optimization of the method for α-l-fucosidase, β-d-galactosidase and
           β-d-glucuronidase determination in serum from hemolyzed blood
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Sylwia Chojnowska, Iwona Ptaszyńska-Sarosiek, Alina Kępka, Sławomir Dariusz Szajda, Napoleon Waszkiewicz, Krzysztof ZwierzAbstractPurposeAdaptation of the colorimetric method for the determination of β-d-galactosidase, β-d-glucuronidase and α-l-fucosidase activities in serums from hemolyzed blood, the material currently being discarded.Materials and MethodsThe materials included serums from hemolyzed and non-hemolyzed blood, obtained from 26 healthy volunteers. The adaptation of the method involved precipitation of the proteins with trichloroacetic acid after incubating serums with substrates, but before determining the products of enzymatic reactions.ResultsIn serums from hemolyzed and non-hemolyzed blood of the same persons, we found high correlations among the results obtained using hemolyzed blood (with adapted) and non-hemolyzed blood (with non-adapted) methods.ConclusionWe are able to determine the β-d-galactosidase, β-d-glucuronidase and α-l-fucosidase activities in serums from hemolyzed blood (with adapted) and non-hemolyzed blood (with non-adapted) methods, with the same accuracy and precision.
  • Assessment of human 4-hydroxynonenal, 8-isoprostane concentrations and
           glutathione reductase activity after synbiotics administration
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Paulina Kleniewska, Rafał PawliczakAbstractPurposeProbiotics and prebiotics have become an object of intense research, to identify methods of mitigating oxidative stress. Over the past few years, the number of in vitro and in vivo studies, related to antioxidant properties of probiotics/prebiotics has significantly increased. The aim of the present study was to assess whether probiotic in combination with prebiotic influences the level of human 4-hydroxynonenal, 8-isoprostane and glutathione reductase activity.Material/methodsExperiments were carried out on healthy volunteers (male and female). All oxidative stress markers were measured in blood plasma pre- and post-administration of synbiotic.ResultsThe administration of synbiotic resulted in a significant decrease in 4-hydroxynonenal in the female-synbiotic group (p 
  • Expression profile and cellular localizations of mucin proteins, CK7, and
           cytoplasmic p27 in Barrett’s esophagus and esophageal adenocarcinoma
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): H. Ece Arslan Ozcan, Turgut Anuk, Ozkan OzdenAbstractPurposeBarrett's esophagus is one of the main risk factors for increased incidence of esophageal adenocarcinoma. In this study, we studied protein expression levels and cellular localizations of MUC-1, MUC-2, MUC-5AC, CK7, and cytoplasmic p27 to assess the relationship between the expression of each of these proteins and the disease progression on endoscopic biopsies.Materials and methodsImmunohistochemical analyses were performed using antibodies produced against MUC-1, MUC-2, MUC-5AC, CK7, and p27. Endoscopic specimens of esophageal mucosa were obtained from 72 patients who underwent esophagectomy for Barrett’s esophagus, metaplasia, dysplasia, or esophageal adenocarcinoma developed from Barrett’s esophagus.ResultsMultilayer squamous epithelium showed only MUC-1 positivity in the EAC group while MUC-2 and MUC-5AC staining could not be detected in this group. Strong and diffused membranous or cytoplasmic staining of CK7 was observed at squamous, ductal, surface columnar and/or glandular epithelium. c-p27 staining was diffused and moderate in the cellular membranes observed in all groups except for esophageal epithelial metaplasia without intestinal metaplasia. Additionally, weakly focal cytoplasmic staining in squamous epithelium of p27 in EAC was detected.ConclusionsBarrett's esophagus, which has a heterogeneous epithelium, might yield different diagnosis based on endoscopic evaluation and immunohistological investigation. Thus, the use of MUC1, p27, and CK7 might strengthen the truthful diagnosis. MUC-1, CK7, and c-p27 immunostaining can be used as the predictive markers for esophageal cancer progression from Barrett’s esophagus.
  • Prevalence of Clostridium difficile infection in hospitalized patients
           with diarrhoea: Results of a Polish multicenter, prospective, biannual
           point-prevalence study
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Hanna Pituch, Piotr Obuch-Woszczatyński, Dominika Lachowicz, Robert Kuthan, Katarzyna Dzierżanowska-Fangrat, Agnieszka Mikucka, Katarzyna Jermakow, Aleksandra Pituch-Zdanowska, Kerrie Davies, Polish EUCLID C. difficile Study GroupAbstractPurposeWe aimed to measure the underdiagnosis of Clostridium difficile infection across Poland and the distribution of PCR-ribotypes of C. difficile.Material and methodsTwenty seven Polish healthcare facilities (HCFs) participated in this prospective study. Each HCF systematically sent all diarrhoeal stools received from inpatients at their laboratories on two days (one in January 2013 and one in July 2013), independently of CDI test request, to the National Coordinating Laboratory (NCL) for standardized testing of CDI. Positive samples (using two-stage algorithm), had CDI, confirmed by qPCR and toxigenic culture. C. difficile isolates were characterized by PCR-ribotyping. Hospitals were questioned about their methods and testing policy for CDI during the study period: September 2011 to August 2013.ResultsDuring the study period, participating hospitals reported a mean of 33.2 tests for CDI per 10 000 patient-days and a mean of 8.4 cases of CDI per 10 000 patient-days. The overall prevalence of positive CDI patients at NCL was 16.5%. Due to absence of clinical suspicion, 19.1% of these patients were not diagnosed by the local diagnostic laboratory. We identified 23 different PCR-ribotypes among 87C. difficile strains isolated from patients. PCR-ribotype 027 (48%) was the most prevalent.ConclusionsThe incidence of CDI in Poland in study period was very high. It should be noted however, that there is a lack of clinical suspicion and underestimation of the need to perform diagnostic tests for CDI in hospitalized patients. This will have an impact on the reported epidemiological status of CDI in Poland.
  • Aspartic acid functionalized PEGylated MSN@GO hybrid as an effective and
           sustainable nano-system for in-vitro drug delivery
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Reza Rahmatolahzadeh, Masood Hamadanian, Leila Ma’mani, Abbas ShafieeAbstractPurposeIn this research, aspartic acid functionalized PEGylated mesoporous silica nanoparticlesgraphene oxide nanohybrid (As-PEGylated-MSN@GO) prepared as a pH-responsive drug carrier for the curcumin delivery. For better camouflage during blood circulation, poly(ethylene glycol) was decorated on the surface of MSN@GO nanohybrid.Materials and methodsThe nanocarrier was characterized by using X-ray powder diffraction (XRD), dynamic light scattering (DLS), UV–vis spectroscopy, thermal gravimetry analysis (TGA), FT-IR, SEM and TEM.ResultsThe size of modified MSN@GO was around 75.8 nm and 24% wt. of curcumin was loaded on the final nanohybrid. pHdecrement from 7.4 to 5.8 the release medium led to increase the cumulative amount of drug release from 54% to 98%.ConclusionsAs-functionalized MSN@GO had no cytotoxicity against human breast adenocarcinoma (MCF-7) and human mammary epithelial (MCF10A) as cancerous and normal cell lines, respectively. Whereas curcuminloaded nanohybrid showed excellent killing capability against MCF-7 cells.
  • A simplified approach for evaluating sustained slow pathway conduction for
           diagnosis and treatment of atrioventricular nodal reentry tachycardia in
           children and adults
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Janusz Sledz, Michal Labus, Mariusz Mazij, Monika Klank-Szafran, Dariusz Karbarz, Bartosz Ludwik, Jacek Kusa, Karol Deutsch, Leslaw Szydlowski, Adrian Mscisz, Jerzy Spikowski, Aleksandra Morka, Tomasz Kameczura, Aleksandra Swietoniowska-Mscisz, Sebastian StecAbstractPurposeDuring incremental atrial pacing in patients with atrioventricular nodal reentrant tachycardia, the PR interval often exceeds the RR interval (PR > RR) during stable 1:1 AV conduction. However, the PR/RR ratio has never been evaluated in a large group of patients with pacing from the proximal coronary sinus and after isoproterenol challenge. Our study validates new site of pacing and easier method of identification of PR > RR.Material and methodsA prospective protocol of incremental atrial pacing from the proximal coronary sinus was carried out in 398 patients (AVNRT-228 and control-170). The maximum stimulus to the Q wave interval (S-Q = PR), SS interval (S-S), and Q-Q (RR) interval were measured at baseline and 10 min after successful slow pathway ablation and after isoproterenol challenge (obligatory).ResultsThe mean maximum PR/RR ratios at baseline were 1.17 ± 0.24 and 0.82 ± 0.13 (p 
  • The preliminary association study of ADIPOQ, RBP4, and BCMO1 variants with
           polycystic ovary syndrome and with biochemical characteristics in a cohort
           of Polish women
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Ewa Czeczuga-Semeniuk, Marzenna Galar, Katarzyna Jarząbek, Piotr Kozłowski, Nela A. Sarosiek, Sławomir WołczyńskiAbstractPurposeWe aimed to elucidate the frequency of the SNPs in the ADIPOQ, RBP4 and BCMO1genes in a population of Caucasian Polish women with polycystic ovary syndrome (PCOS), and to evaluate the possible associations between these variants and the susceptibility to PCOS. Additionally, the relationship of these polymorphisms to a clinical phenotype of this syndrome, and the concentrations of adipokines, were determined.Materials/methodsClinical and biochemical profiles, DNA isolation and genotyping, and adipokine assays were performed in 294 PCOS women and 78 controls.ResultsIn a cohort of Polish women, for the genotype distribution and allele frequencies (minor allele frequency − MAF) proved that only the SNP rs1501299 in the gene ADIPOQ (P = 0.0010, OR = 0.41, 95% C.I.:0.24-0.70) and rs7501331 in the gene BCMO1 (P = 0.0106, OR = 0.24, 95% C.I.:0.21-0.71), are significantly associated (the latter marginally significant) with the decrease of the risk of the disease. Also for this SNPs there were significant differences in the genotypic frequencies in the study population.There was a link between rs12934922 of BCMO1 gen and serum concentration of RBP4 (P = 0.034) and adiponectin (P = 0.038) in the study group but not in the control group. The elevated mean serum concentration of cholesterol (P = 0.020) and LDL cholesterol (P = 0.005) was observed for GG rs1501299 genotype and triglycerides (P = 0.028) for TT rs2241766 genotype.ConclusionsThe results of the present study revealed that the genes variants RBP4 is not associated with PCO. It seems that rs1501299 of ADIPOQ gene influences the occurrence of PCO and lipids profile in those patients.
  • Urinary exoglycosidases, reference values in healthy children
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Beata Zalewska-Szajda, Katarzyna Taranta-Janusz, Sylwia Chojnowska, Napoleon Waszkiewicz, Krzysztof Zwierz, Anna WasilewskaAbstractPurposeThe purpose of the study was to determine the effect of age on lysosomal exoglycosidase activities: α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase in healthy children and adolescents.Material and methodsUrine samples were collected from 203 healthy children and adolescents (girls = 99, boys = 104), aged six months to 17.9 years. The activities of α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase were determined by colorimetric method and expressed in pKat/μg of creatine (pKat/μg Cr.).ResultsUrinary α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase activities (pKat/μg Cr.) were the highest in children below 3 years of age in comparison to the remaining age groups. There was a statistically significant negative correlation between urinary α-fucosidase, β-galactosidase, β-glucuronidase and α-mannosidase (pKat/μg Cr.) and age (r = −0.36; r = −0.36; r = −0.35; r = −0.35; at p 
  • TGF-β and inflammatory blood markers in prediction of intraperitoneal
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Kamil Torres, Łukasz Pietrzyk, Zbigniew Plewa, Karolina Załuska-Patel, Mariusz Majewski, Elżbieta Radzikowska, Anna TorresAbstractPurposeIntraperitoneal adhesions (IA) develop as a consequence of the healing process in peritoneum injured during surgeries. IA might be formed after all types of surgical interventions regardless the surgical approach with a higher incidence in obese individuals. Here we determine the diagnostic power of TGF-β and blood inflammatory parameters in the prediction of IA in obese patients undergoing second surgical intervention.Materials and methodsEighty patients were divided into groups according to body mass index (BMI) values and presence of intraperitoneal adhesions (IA). Evaluation of peritoneal adhesion index (PAI), serum TGF-β and blood inflammatory parameters was performed.ResultsLevel of TGF-β, C-reactive protein (CRP), leukocytes, neutrophil to lymphocyte ratio and platelet to lymphocyte ratio were significantly higher in obese patients while TGF-β, CRP, and leukocytes were higher in patients with IA. There was a significant correlation between PAI values and TGF-β concentration (p 
  • Activation of mitochondrial KATP channels mediates neuroprotection induced
           by chronic morphine preconditioning in hippocampal CA-1 neurons following
           cerebral ischemia
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Maedeh Arabian, Nahid Aboutaleb, Mansoureh Soleimani, Marjan Ajami, Rouhollah Habibey, Hamidreza Pazoki-ToroudiPurposePharmacologic preconditioning, through activating several mechanisms and mediators, can increase the tolerance of different tissues against ischemia/reperfusion (I/R) injury. Recent studies have shown that morphine preconditioning has protective effects in different organs, especially in the heart. Nevertheless, its mechanisms are not well elucidated in the brain. The present study aimed to clarify whether the activation of mitochondrial KATP (mKATP) channels in chronic morphine (CM) preconditioning could decrease hippocampus damage following I/R injury.Materials and methodsCM preconditioning was performed by the administration of additive doses of morphine for 5 days before I/R injury induction. I/R injury was induced by the occlusion of bilateral common carotid arteries. The possible role of mKATP channels was evaluated by the injection of 5-hydroxydecanoate (5-HD) before I/R injury. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) was performed to detect apoptosis in hippocampal neurons. The expressions of B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (BAX) and levels of malondialdehyde (MDA) and catalase (CAT) enzymes were assessed.ResultsCM attenuated apoptosis in the hippocampal CA1 neurons (P 
  • The role of platelets in the development and progression of pulmonary
           arterial hypertension
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Remigiusz Kazimierczyk, Karol KamińskiAbstractPulmonary arterial hypertension is a multifactorial disease characterized by vasoconstriction, vascular remodeling, inflammation and thrombosis. Although an increasing number of research confirmed that pulmonary artery endothelial cells, pulmonary artery smooth muscle cells as well as platelets have a role in the pulmonary arterial hypertension pathogenesis, it is still unclear what integrates these factors. In this paper, we review the evidence that platelets through releasing a large variety of chemokines could actively impact the pulmonary arterial hypertension pathogenesis and development. A recent publication revealed that not only an excess of platelet derived cytokines, but also a deficiency may be associated with pulmonary arterial hypertension development and progression. Hence, a simple platelet blockade may not be a correct action to treat pulmonary arterial hypertension. Our review aims to analyse the interactions between the platelets and different types of cells involved in pulmonary arterial hypertension pathogenesis. This knowledge could help to find novel therapeutic options and improve prognosis in this devastating disease.
  • Interactions between the growth hormone and cytokines – A review
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Mieczysław Szalecki, Anna Malinowska, Monika Prokop-Piotrkowska, Roman JanasAbstractNumerous reports on the interactions between the immune and endocrine systems, especially growth hormone axis, can be found in the literature. Growth hormone acts mainly indirectly through insulin-like growth factor-1, which stimulates the growth and development processes, metabolism of lipids, proteins, and carbohydrates, and it also has a modulating effect on the cells of the immune system.Several studies have been conducted on the influence of growth hormone therapy on the immunological parameters in children and adults with and without growth hormone deficiency. However, there have been no definite results and some of them have been even contradictory. Some studies have suggested that administration of growth hormone increases the production of tumor necrosis factor and certain pro- and anti-inflammatory cytokines; whereas other studies have demonstrated the lack of correlation between growth hormone and interleukins.The aim of this paper was to evaluate the available literature on the interaction between growth hormone and TNF-α, pro-inflammatory (IL-1β, IL-2, IL-6) and anti-inflammatory (IL-4, IL-10) interleukins.
  • Th9 lymphocytes and functions of interleukin 9 with the focus on IBD
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Krzysztof Matusiewicz, Barbara Iwańczak, Małgorzata MatusiewiczAbstractThe work presents the newest knowledge on a new phenotype of T helper lymphocytes (Th9) and on Interleukin 9 (IL-9). Processes leading to transformation of naïve T lymphocyte into Th9 lymphocytes are presented, including the role of IL-4 and TGFβ signaling. Involvement of transcription factor network in production of IL-9 is described. Other cells capable of expressing IL-9 and secreting IL-9 are portrayed. Diversity of IL-9 effects caused by activation of IL-9 receptors on various types of cells is presented. Principal effects of the activation of IL-9 receptor on T-cells seem to be antiapoptotic and stimulatory which leads to enhanced defense against parasitic infection and cancer development but, from the other side, it perpetuate chronic inflammation in autoimmune diseases and allergic processes. In the last years the role of IL-9 in autoimmune diseases such as rheumatic diseases and inflammatory bowel disease gained importance since the increased expression of this cytokine has been observed in animal models of intestinal inflammation and in groups of patients with ulcerative colitis. It was also noted that neutralization of IL-9 in animal models of ulcerative colitis leads to amelioration of inflammatory process, what could have significance in the treatment of this disease in humans in the future.
  • Markers of anaphylaxis – a systematic review
    • Abstract: Publication date: September 2018Source: Advances in Medical Sciences, Volume 63, Issue 2Author(s): Maria Magdalena Tomasiak-Łozowska, Maciej Klimek, Agnieszka Lis, Marcin Moniuszko, Anna Bodzenta-ŁukaszykAbstractAnaphylaxis is defined as severe, life-threatening, systemic or general, immediate reaction of hypersensitivity, with repeatable symptoms caused by the dose of stimulus which is well tolerated by healthy persons. The proper diagnosis, immediate treatment and differential diagnosis are crucial for saving patient's life. However, anaphylaxis is relatively frequently misdiagnosed or confused with other clinical entities. Thus, there is a continuous need for identifying detectable markers improving the proper diagnosis of anaphylaxis. Here we presented currently known markers of anaphylaxis and discussed in more detail the most clinically valuable ones: tryptase, platelet activacting factor (PAF), PAF-acethylhydrolase, histamine and its metabolites.
  • Lotus tetragonolobus and Maackia amurensis lectins influence
           phospho-IκBα, IL-8, Lewis b and H type 1 glycoforms levels in H. pylori
           infected CRL-1739 gastric cancer cells
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Iwona Radziejewska, Małgorzata Borzym-Kluczyk, Katarzyna Leszczyńska, Joanna Wosek, Anna BielawskaAbstractPurposeAttachment of Helicobacter pylori to the mucous epithelial cells and the mucous layer is said to be a crucial step for infection development. Sugar antigens of gastric mucins (MUC5AC, MUC1) can act as receptors for bacterial adhesins. The aim of the study was to investigate if Lotus tetragonolobus and Maackia amurensis lectins influence the level of MUC1, MUC5AC, Lewis b, H type 1, sialyl Lewis x, phospho-IκBα and interleukin 8 in Helicobacter pylori infected gastric cancer cells.Materials and methodsThe study was performed with one clinical H. pylori strain and CRL-1739 gastric cancer cells. To assess the levels of mentioned factors immunosorbent ELISA assays were used.ResultsCoculture of cells with bacteria had no clear effect on almost all examined structures. After coculture with H. pylori and lectins, a decrease of the level of both mucins, Lewis b and H type 1 antigens was observed. Lectins addition had no effect on sialyl Lewis x. Maackia amurensis caused slight increase of phospho-IκBα while interleukin 8 level was decreased.ConclusionsLotus tetragonolobus and Maackia amurensis lectins can mediate in binding of Helicobacter pylori to gastric epithelium.
  • Is the Clock Drawing Test useful in the screening assessment of aged
           patients with chronic heart failure'
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Karolina Piotrowicz, Małgorzata Fedyk-Łukasik, Anna Skalska, Tomasz GrodzickiAbstractPurposeCognitive impairment is one of the most common geriatric deficits in old patients with heart failure (HF), but there has been a lack of study on the utility of the Clock Drawing Test (CDT) when used with this group of patients. The aim of the study was to assess the usefulness of the CDT in the geriatric assessment of aged outpatients with chronic HF.Patients and methodsA cross-sectional analysis of the results of the comprehensive geriatric assessment (CGA), including the CDT, of 92 aged outpatients with heart failure was conducted.ResultsWe found a high prevalence of five examined geriatric problems. The majority of the patients presented signs of cognitive deterioration of different patterns and severity on the Clock Drawing Test. All the CDT scoring systems correlated significantly with the Mini-Mental Test Examination results.ConclusionsIt seems reasonable to perform the routine CGA with the CDT examination in all aged heart failure patients.
  • Perioperative thrombocytopenia predicts poor outcome in patients
           undergoing transcatheter aortic valve implantation
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Maciej Mitrosz, Remigiusz Kazimierczyk, Malgorzata Chlabicz, Bozena Sobkowicz, Ewa Waszkiewicz, Anna Lisowska, Slawomir Dobrzycki, Wlodzimierz J. Musial, Tomasz Hirnle, Karol A. Kaminski, Agnieszka M. TycinskaAbstractPurposeTo determine the time point at which thrombocytopenia after TAVI procedure is an indicator of the worst prognosis, with special consideration of perioperative platelet and coagulation activation as its potential causes.MethodsThirty two patients (mean age 78.5 ± 7.9 years, 62% females) qualified for TAVI procedure were prospectively evaluated. Platelet counts were assessed at baseline and for the next three postoperative (POD) days. Platelet activation was evaluated by P-selectin (PS, serum, ELISA) and platelet factor 4 (PF-4, CTAD plasma), and blood coagulation activation by prothrombin fragments 1 + 2 (F1 + 2, plasma, ELISA). Composite end point (CEP) including death and the need of cardiovascular rehospitalization was assessed after a mean of 14.1 ± 6.7 months.ResultsDuring the follow up period half of the patients reached CEP. Thrombocytopenia was more profound and frequent in patients with CEP as compared to those without (p 
  • Vitamin D deficiency in children with recurrent respiratory infections,
           with or without immunoglobulin deficiency
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Nel Dąbrowska-Leonik, Ewa Bernatowska, Małgorzata Pac, Wiktor Filipiuk, Jan Mulawka, Barbara Pietrucha, Edyta Heropolitańska-Pliszka, Katarzyna Bernat-Sitarz, Beata Wolska-Kuśnierz, Bożena MikołućAbstractPurposeThe objective of this study was to evaluate thevitamin D concentration in patients with recurrent respiratory infections with or without immunoglobulin G, A or M (IgG, IgA, IgM) deficiency, and to find a correlation between the vitamin D concentration and the response to hepatitis B vaccination.Materials and methodThe study involved 730 patients with recurrent respiratory infections. The concentration of 25-hydroxyvitamin D (25(OH)D), immunoglobulins G, A and M, anti-HBs was determined.ResultsThe tests showed that 11% of patients presented IgG levels below the age related reference values. Children with reduced IgG concentration were also found to have significantly lower vitamin D concentrations in comparison to children with normal IgG. Vitamin D deficiency was observed in schoolchildren between 7 and 18 years of age. No correlation was found between 25(OH)D concentration and Hbs antibody levels.ConclusionsAn investigation of a large group of patients who have recurrent infection found patients with IgG deficiency to whom special proceeding have to be performed: 1. Significantly lower vitamin D concentration observed in the group of children with IgG deficiency implicated in long-lasting monitoring of vitamin D level require adding to the practice guidelines for Central Europe 2013. 2. Intervention treatment with suitable doses of vitamin D to clarified metabolism of vitamin D has to be plan for children with IgG deficiency and significant lower vitamin D concentration.
  • Tick-borne infections and co-infections in patients with non-specific
           symptoms in Poland
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Justyna Dunaj, Anna Moniuszko-Malinowska, Izabela Swiecicka, Martin Andersson, Piotr Czupryna, Krzysztof Rutkowski, Grzegorz Zambrowski, Joanna Zajkowska, Sambor Grygorczuk, Maciej Kondrusik, Renata Świerzbińska, Sławomir PancewiczAbstractAimThe aim of the study was the evaluation of the frequency of infections and co-infections among patients hospitalized because of non-specific symptoms after a tick bite.Materials and methodsWhole blood, serum and cerebrospinal fluid samples from 118 patients hospitalised for non-specific symptoms up to 8 weeks after tick bite from 2010 to 2013 were examined for tick-borne infections. ELISA, Western blot and/or molecular biology (PCR; fla gene; 16S rRNA; sequencing) and thin blood smears (MDD) were used. Control group included 50 healthy blood donors. All controls were tested with PCR and serology according to the same procedure as in patients.ResultsOut of 118 patients 85 (72%) experienced headaches, 15 (13%) vertigo, 32 (27%) nausea, 17 (14%) vomiting, 37 (31%) muscle pain, 73 (62%) fever and 26 (22%) meningeal signs. 47.5% were infected with at least one tick-borne pathogen. Borrelia burgdorferi sensu lato infection was confirmed with ELISA, Western blot in serum and/or (PCR (fla gene) in whole blood in 29.7% cases. In blood of 11.9% patients Anaplasma phagocytophilum DNA (16S rRNA gene) was detected; in 0.9% patients 1/118 Babesia spp. DNA (18S rRNA gene) was also detected. Co-infections were observed in 5.1% of patients with non-specific symptoms. B. burgdorferi s.l. − A. phagocytophilum co-infection (5/118; 4.2%) was most common. In 1/118 (0.8%) A. phagocytophilum – Babesia spp. co-infection was detected. All controls were negative for examined pathogens.ConclusionsNon-specific symptoms after tick bite may be caused by uncommon pathogens or co-infection, therefore it should be considered in differential diagnosis after tick bite.
  • Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A
           link between osteopontin and cardiovascular events
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Paweł Rubiś, Sylwia Wiśniowska-Śmiałek, Ewa Dziewięcka, Lucyna Rudnicka-Sosin, Artur Kozanecki, Piotr PodolecAbstractIntroductionSerum markers of fibrosis provide an insight into extracellular matrix (ECM) fibrosis in heart failure (HF) and dilated cardiomyopathy (DCM). However, their role as predictors of cardiovascular (CV) events in DCM is poorly understood.MethodsThis is an observational, prospective cohort study. 70 DCM patients (48 ± 12.1 years, ejection fraction – EF 24.4 ± 7.4) were recruited. Markers of collagen type I and III synthesis – procollagen type I and III carboxy- and amino-terminal peptides (PICP, PIIICP, PINP, PIIINP), fibrosis controlling factors – ostepontin (OPN), transforming growth factor (TGF1-β) and connective tissue growth factor (CTGF), and matrix metalloproteinases (MMP-2, MMP-9) and tissue inhibitor (TIMP-1), were measured in serum. All patients underwent endomyocardial biopsy. The end-point was combined with CV death and urgent HF hospitalization. Patients were divided into two groups: those who did (group 1, n = 45) and did not reach (group 2, n = 25) an end-point.ResultsOver a 12-month period of observation, 6 CV deaths and 19 HF hospitalizations occurred. Qualitative and quantitative measures of ECM fibrosis were similar in both groups. The levels of all of the markers of collagen synthesis, TGF1-β, MMP-9 and TIMP-1 were similar, however, OPN, CTGF and MMP-2 were significantly lower in group 1.ConclusionsInvasively-determined fibrosis levels were not related with CV outcomes in DCM. Out of the 11 markers of fibrosis under study, only OPN was found to be related to CV outcomes. OPN is not only the pivotal protein controlling fibrosis, but may also serve as a biomarker associated with prognosis.
  • The safety and efficacy of light emitting diodes-based ultraviolet A1
           phototherapy in bleomycin-induced scleroderma in mice
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Diana Karpec, Romualdas Rudys, Laima Leonaviciene, Zygmunt Mackiewicz, Ruta Bradunaite, Gailute Kirdaite, Algirdas VenalisAbstractPurposeTo define the efficacy and safety of narrowband ultraviolet A1 (UVA1) for the treatment of dermal fibrosis in bleomycin-induced mouse model of scleroderma.Materials and methods42 DBA/2 strain mice were included in the study: healthy mice and mice with established scleroderma, treated with high or medium dose of UVA1. Non-treated groups served as control. The equipment emitting 365 ± 5 nm UVA1 radiation was used in the study. The average cumulative doses were 1200 J/cm2 for high and 600 J/cm2 for medium dose course. Histological analysis was performed for the evaluation of the dermal thickness and mast cells density. The expressions of p53 and Ki-67 proteins were assessed by immunohistochemical analyses.ResultsSkin thickness of mice with scleroderma, treated with high and medium dose of UVA1, were lower (272.9 ± 113.2 μm and 394 ± 125.9 μm, respectively) in comparison to the dermal thickness of non-treated animals (599 ± 55.7 μm). The dermal mast cells count in mice with scleroderma was reduced after high and medium dose treatment to 11 ± 1.7 and 13 ± 2.2, respectively, as compared to that in non-treated mice (23 ± 3.0). No significant upregulation of p53 nor Ki-67 proteins was observed in the skin of healthy mice and mice with scleroderma after high- and medium-dose of UVA1.ConclusionsThe results of this study indicate that 365 nm UVA1 with the cumulative doses of 1200 J/cm2 and 600 J/cm2 is safe and effective for the dermal fibrosis treatment.
  • PPARγ Pro12Ala and C161T polymorphisms in patients with acne vulgaris:
           Contribution to lipid and lipoprotein profile
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Shohreh Saeidi, Foroogh Chamaie-Nejad, Ali Ebrahimi, Fariba Najafi, Ziba Rahimi, Asad Vaisi-Raygani, Ebrahim Shakiba, Zohreh RahimiAbstractPurposeThe aim of present study was to clarify the role of peroxisome proliferator-activated receptor γ (PPARγ) Pro12Ala and C161T variants in the pathogenesis of acne vulgaris (AV) and their influence on lipid and lipoprotein profile.MethodsThe present case-control study consisted of 393 individuals including 198 patients with AV (mild-, moderate-, and severe-AV) and 195 unrelated age-matched healthy individuals from Western Iran. The PPARγ Pro12Ala and C161T polymorphisms were identified using polymerase chain reaction-restriction length polymorphism method. Also, serum lipid and lipoprotein profile and fasting blood sugar (FBS) were detected in studied individuals.ResultsIn women patients with AV significantly higher serum levels of FBS, total cholesterol, low density lipoprotein-cholesterol (LDL-C) and high density lipoprotein-cholesterol compared to healthy women were detected. Neither PPARγ Pro12Ala nor C161T polymorphism was associated with the risk of AV but the Pro allele was a risk factor for AV among all men and women patients ≥20 years. The variant genotype of PPARγ CG (Pro/Ala) was associated with significantly higher levels of total cholesterol and triglycerides compared to CC (Pro/Pro) genotype. We detected a significantly lower level of FBS in the presence of CT + TT genotype of PPARγ C161T compared to CC genotype. Also, carriers of PPARγ TT genotype had significantly lower serum level of total cholesterol and LDL-C compared to CC genotype.ConclusionsOur results demonstrated the association of PPARγ Pro allele with susceptibility to AV in patients ≥20 years and the influence of PPARγ Pro12Ala and C161T polymorphisms on the lipid and lipoprotein profile.
  • The application of L-PRP in AIDS patients with crural chronic ulcers: A
           pilot study
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Agata Cieslik-Bielecka, Rafał Skowroński, Magdalena Jędrusik-Pawłowska, Marcin PierchałaAbstractPurposeNonhealing wounds or skin ulcerations are the result of insufficient repair and destruction of a local healing potential. Opportunistic infections which cause a lot of ulcer complications influence the worsening general condition of patients with AIDS, ultimately leading to death. The chronicity of the condition and poor results of conventional therapy have prompted the search for new methods of treatment.Materials and methodsWe have examined venous or arteriovenous insufficiency-related extensive crural ulcers in AIDS patients. Crural ulcer healing processes were evaluated with clinical observations and histopathological, immunohistochemical and molecular examinations of tissue samples harvested from the wound edges before and on day 10 after L-PRP cover dressing.ResultsClinical observations showed positive effects of L-PRP in all patients. However, complete wound closure was noted in 60% of cases. Statistical analysis of histological examination showed increased epidermal processes between samples, but the difference was nonsignificant. However, immunohistochemical investigations showed an increased healing process with strong statistical significance. The mean VEGF level before L-PRP usage was 114.3 vessels/mm2 and on day 10 118.9 (p = 0.001523). The mean FLK level was 103.2 and 109.9 respectively (p = 0.008241). The biggest differences were observed for CD34, with values of 68.2 on day 0 and 100.8 on day 10 (p = 0.006982). Molecular analysis generally showed decreased gene expression and confirmed vascular formation and reepithelialization processes.ConclusionsIn our opinion, L-PRP may be used to eradicate microorganisms from wounds, to induce neovascularization, and in unhealed cases prepare the base and edge of the ulcer for skin grafting and tissue expansion procedures.
  • The protective effects of Bacillus licheniformis preparation on
           gastrointestinal disorders and inflammation induced by radiotherapy in
           pediatric patients with central nervous system tumor
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Shu-Xu Du, Yong-Rui Jia, Si-Qi Ren, Xiao-Jun Gong, Hong Tang, Wu Wan-Shui, Sun Li-MingAbstractPurposewe studied the effect of Bacillus licheniformis preparation (ZCS) on CNST (central nervous system tumor) patients undergoing the gastrointestinal symptoms and inflammation induced by radiotherapy.Materials and Methods160 CNST patients with craniospinal irradiation (CSI) treatment were divided into experiment and control group. The experiment group patients took one capsule per time of ZCS and three times a day until the end of radiotherapy, starting one day before radiotherapy. While the patients in control group were administrated placebo without any probiotics. Serum from one day before radiotherapy and the first day after radiotherapy were collected to measure the ET, CRP, TNF-α, IL-1β and IL-6.ResultsMore than 70% CNST pediatric patients suffered from different degrees of gastrointestinal symptoms after radiotherapy, including mouth ulcer, nausea, vomiting, abdominal pain and diarrhea. And there was an obviously increased of serum ET, TNF-α, IL-1β, IL-6 and CRP after RT. Importantly, a markedly decreased of ET, CRP and inflammatory cytokines were detected in the experiment group comparing to the control group after radiotherapy, as well as the relief of the gastrointestinal symptoms. However, improvement of probiotics (or ZCS) of the survival rate of CNST children and the recurrence of tumor are not observed in this study.ConclusionsProphylactically administrated ZCS during radiotherapy for CNST patients can relieve RT-related gastrointestinal symptoms and inflammatory reaction.
  • Ultrastructural characteristics of the respective forms of hepatic
           stellate cells in chronic hepatitis B as an example of high fibroblastic
           cell plasticity. The first assessment in children
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Joanna Maria Lotowska, Maria Elzbieta Sobaniec-Lotowska, Dariusz Marek LebensztejnAbstractPurposeActivation of hepatic stellate cells (HSCs), mainly responsible for extracellular matrix synthesis, is assumed to be central event in the process of liver fibrogenesis. The major objective of the research was to analyze the ultrastructural profile of activated HSCs in children with chronic hepatitis B (chB), with respect to fibrosis intensity.Materials/methodsUltrastructural investigations of HSCs were conducted on liver bioptates from 70 children with clinicopathologically diagnosed chB before antiviral treatment. Biopsy material, fixed in paraformaldehyde and glutaraldehyde solution, was routinely processed for electron-microscopic analysis.ResultsIn children with intensive liver fibrosis (S-2 and S-3), the ultrastructural picture showed almost total replacement of quiescent HSCs (Q-HSCs) by activated, i.e. transitional HSCs (T-HSCs). Among T-HSCs, two types of cells were distinguished: cells exhibiting initiation of HSC activation (Ti-HSCs), never before described in chB, that were frequently accompanied by activated Kupffer cells, and cells with features of perpetuation of activation (Tp-HSCs). Tp-HSCs were elongated and characterized by substantial loss of cytoplasmic lipid material; they contained an increased number of cytoskeletal components, extremely dilated channels of granular endoplasmic reticulum and activated Golgi apparatus, which indicated their marked involvement in intensive synthesis of extracellular matrix proteins. Many collagen fibers were found to adhere directly to Tp-HSCs.ConclusionsThe current study showed T-HSCs to be an important link between Q-HSCs and myofibroblastic HSCs (Mf-HSCs). Transformation of HSCs into new morphological variations (Ti-HSCs; Tp-HSCs and Mf-HSCs), observed along with growing fibrosis, indicates their high plasticity and a key role in fibrogenesis in pediatric chB.
  • Effect of gestational age on migration ability of the human umbilical cord
           vein mesenchymal stem cells
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Mobin Mohammadi, Mehdi Mohammadi, Mohammad Ali Rezaee, Tayyeb Ghadimi, Massume Abolhasani, Mohammad Reza RahmaniAbstractPurposeMigration ability of mesenchymal stem cells (MSCs) towards chemotactic mediators is a determinant factor in cell therapy. MSCs derived from different sources show different properties. Here we compared the migration ability of the term and the pre-term human umbilical cord vein MSCs (hUCV-MSCs).Materials/MethodsMSCs were isolated from term and pre-term umbilical cord vein, and cultured to passage 3–4. Migration rate of both groups was assessed in the presence of 10% FBS using chemotaxis assay. Surface expression of CXCR4 was measured by flow cytometery. The relative gene expression of CXCR4, IGF1-R, PDGFRα, MMP-2, MMP-9, MT1-MMP and TIMP-2 were evaluated using real time PCR.ResultsThe isolation rate of the pre-term hUCV-MSCs was higher than the term hUCV-MSCs. Phenotype characteristics and differentiation ability of the term and pre-term hUCV-MSCs were not different. The migration rate of the pre-term hUCV-MSCs was more than the term hUCV-MSCs. Gene and surface expressions of the CXCR4 were both significantly higher in the pre-term hUCV-MSCs (P ≤ 0.05). The mRNA levels of PDGFRα, MMP-2, MMP-9, MT1-MMP and TIMP-2 showed no significant difference between the two groups.ConclusionOur results showed that the gestational age can affect the migration ability of the hUCV-MSCs.
  • Physeal fractures of the lower leg in children and adolescents:
           Therapeutic results, pitfalls and suggested management protocol - based on
           the experience of the authors and contemporary literature
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Marcin Karlikowski, Jerzy SułkoAbstractPurposePhyseal fractures in children frequently give rise to concerns about the condition of the growth plate. Our observations have proven that the dysfunction of the growth plate is less frequent complication in those cases than misdiagnosed interposition of the periosteum. The aim of this paper is to familiarize the readers with the issue of treatment of physeal fractures of the distal tibia and fibula in the growing skeleton.Materials and methodsWe analyzed the group of 75 patients – children and adolescents – with surgically treated physeal fractures of the lower leg. The analysis included age, sex, circumstances of trauma infliction, type of sustained damage, employed therapeutic technique, timing of surgical procedure, duration of hospitalization, complications, duration of follow-up, radiological and functional results according to the AOFAS scale.ResultsThe group consisted of 23 girls and 52 boys. The mean age was 13.6 years. The most frequent cause of trauma was same-level fall, usually during sports activities (35 cases). The most common type of damage was Salter-Harris type II fracture (35 cases). Among the employed surgical techniques, open reduction and stabilization with K-wires was the most often used (52 cases). A group of four patients attracted our attention, in whom after a closed reduction, signs of periosteum interposition were noted. These patients required a second procedure. In one patient, the growth plate arrest occurred; it was directly caused by local osteomyelitis.ConclusionsWith adequately conducted treatment of distal tibia and distal fibula physeal fractures, the results are good. Misdiagnosed periosteum interposition poses a more serious clinical problem as opposed to the commonly anxiety-provoking post-traumatic growth plate dysfunction.
  • Pharmacological thrombolysis for acute ischemic stroke treatment: Gender
           differences in clinical risk factors
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Michael J. Colello, Lauren E. Ivey, Jordan Gainey, Rakiya V. Faulkner, Ashleigh Johnson, Leanne Brechtel, Lee Madeline, Thomas I. NathanielAbstractBackgroundIn a stroke population, women have a worse outcome than men when untreated. In contrast, there is no significant difference in treated patients. In this study, we determined whether clinical variables represent a promising approach to assist in the evaluation of gender differences in a stroke population.MethodsWe analyzed data from ischemic stroke patients’ ≥18 years-old from the stroke registry on rtPA administration and identified gender differences in clinical factors within inclusion and exclusion criteria in a stroke population that received rtPA. Multivariate analysis was used to adjust for patient demographic and clinical variables.ResultsOf the 241 eligible stroke patients’ thrombolytic therapy, 49.4% were females and 50.6% were males. Of the 422 patients that did not receive rtPA, more women (235) were excluded from rtPA than men (187) (P 
  • Pediatric reference data on activity of urinary
           N-acetyl-β-D-hexosaminidase and its isoenzymes
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Beata Zalewska-Szajda, Katarzyna Taranta-Janusz, Sylwia Chojnowska, Napoleon Waszkiewicz, Krzysztof Zwierz, Anna WasilewskaAbstractPurposeThe objective of the study was to establish age − dependent values of the urinary lysosomal exoglycosidases activities: N-acetyl-β-D-hexosaminidase (HEX) and its isoenzyme A (HEX A) as well as isoenzyme B (HEX B) in healthy children and adolescents.Material and methodsThe study was performed using a random sample of 203 healthy children and adolescents (girls = 99, boys = 104), aged six months to 17.9 years. The activities of HEX, HEX A and HEX B were determined by a colorimetric method. The activities of the urinary HEX and its isoenzymes were expressed in pKat/μg of creatinine (pKat/μg Cr).ResultsMedian concentrations of urinary HEX, and its HEX A, HEX B isoenzymes in particular age groups were analyzed using ANOVA. Urinary HEX, HEX A and HEX B activities (pKat/μg Cr) were the highest in children below 3 years, in comparison to remaining age groups. There were statistically significant negative correlations between urinary HEX, HEX A as well as HEX B and age (r = −0.24, p 
  • Novel mutations and their genotype-phenotype correlations in patients with
           Noonan syndrome, using next-generation sequencing
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Alireza Tafazoli, Peyman Eshraghi, Francesca Pantaleoni, Rahim Vakili, Morteza Moghaddassian, Martha Ghahraman, Valentina Muto, Stefano Paolacci, Fatemeh Fardi Golyan, Mohammad Reza AbbaszadeganAbstractPurposeNoonan Syndrome (NS) is an autosomal dominant disorder with many variable and heterogeneous conditions. The genetic basis for 20–30% of cases is still unknown. This study evaluates Iranian Noonan patients both clinically and genetically for the first time.Materials/methodsMutational analysis of PTPN11 gene was performed in 15 Iranian patients, using PCR and Sanger sequencing at phase one. Then, as phase two, Next Generation Sequencing (NGS) in the form of targeted resequencing was utilized for analysis of exons from other related genes. Homology modelling for the novel founded mutations was performed as well. The genotype, phenotype correlation was done according to the molecular findings and clinical features.ResultsPreviously reported mutation (p.N308D) in some patients and a novel mutation (p.D155N) in one of the patients were identified in phase one. After applying NGS methods, known and new variants were found in four patients in other genes, including: CBL (p. V904I), KRAS (p. L53W), SOS1 (p. I1302V), and SOS1 (p. R552G). Structural studies of two deduced novel mutations in related genes revealed deficiencies in the mutated proteins. Following genotype, phenotype correlation, a new pattern of the presence of intellectual disability in two patients was registered.ConclusionsNS shows strong variable expressivity along the high genetic heterogeneity especially in distinct populations and ethnic groups. Also possibly unknown other causative genes may be exist. Obviously, more comprehensive and new technologies like NGS methods are the best choice for detection of molecular defects in patients for genotype, phenotype correlation and disease management.
  • A similar pro/anti-inflammatory cytokine balance is present in the airways
           of competitive athletes and non-exercising asthmatics
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Marcin Kurowski, Janusz Jurczyk, Agnieszka Olszewska-Ziąber, Marzanna Jarzębska, Hubert Krysztofiak, Marek L. KowalskiAbstractPurposeIntensive exercise modifies airway inflammation and infection susceptibility. We aimed to determine the effect of exercise on pro- and anti-inflammatory cytokine (TNF-α, IL-1ra, IL-10) and innate immunity protein (HSPA1, sCD14) levels in exhaled breath condensate (EBC) and nasal secretions of competitive athletes, non-exercising asthmatics and healthy controls (HC).Material and methodsThe study group consisted of 15 competitive athletes (five speed skaters and ten swimmers) aged 15–25. The control groups comprised 10 mild-to-moderate asthmatics (AC) and seven HC. Athletes were assessed in- and off-training while asthmatics and controls at one time point. Nasal lavages and EBC were collected before and after a treadmill exercise challenge. Protein levels were assessed using ELISA.ResultsTNF-α levels in EBC were significantly higher in athletes than HC, but similar to asthmatic patients. In contrast, IL-1ra EBC concentrations were significantly lower in athletes than in HC, but again similar to asthmatics. Significant positive correlations were seen between baseline concentrations of TNF-α in EBC and fall in FEV1 following exercise challenge in athletes during training period (R = 0.74, p 
  • Endogenous non-enzymatic antioxidants in the human body
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Iwona Mirończuk-Chodakowska, Anna Maria Witkowska, Małgorzata Elżbieta ZujkoAbstractThe exposure of cells, tissues and extracellular matrix to harmful reactive species causes a cascade of reactions and induces activation of multiple internal defence mechanisms (enzymatic or non-enzymatic) that provide removal of reactive species and their derivatives. The non-enzymatic antioxidants are represented by molecules characterized by the ability to rapidly inactivate radicals and oxidants. This paper focuses on the major intrinsic non-enzymatic antioxidants, including metal binding proteins (MBPs), glutathione (GSH), uric acid (UA), melatonin (MEL), bilirubin (BIL) and polyamines (PAs).
  • The influence of low level laser irradiation on vascular reactivity
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Magdalena Mackiewicz-Milewska, Elżbieta Grześk, Andrzej C. Kroszczyński, Małgorzata Cisowska-Adamiak, Hanna Mackiewicz-Nartowicz, Lilianna Baran, Iwona Szymkuć-Bukowska, Michał Wiciński, Wojciech Hagner, Grzegorz GrześkPurposeThe mechanism of action of low level laser irradiation on tissues is unclear.
      Authors of publications present the positive clinical impact of low and medium power laser irradiation on vascular reactivity. The purpose of this study was to analyze the role of vascular endothelium in laser-induced constricted by endothelin-1 and phenylephrine.Materials and methodsExperiments were performed on isolated and perfused rat tail arteries of weighing 250–350 g male Wistar rats. Contractility of arteries as a response to endothelin-1 and phenylephrine was measured after exposure to laser stimulation (10, 30 and 110 mW).ResultsLaser irradiation inhibits vascular smooth muscle contraction induced by endothelin-1 and an alpha-adrenergic receptor agonist, phenylephrine proportionally to the laser power. Concentration-response curves were shifted to the right with significant reduction in maximal response. Laser irradiation at the power of 10 mW, 30 mW, and 110 mW reduced the maximum response of arteries stimulated with phenylephrine sequentially to 88%, 72%, and 52%. Similar findings were observed during stimulation of endothelin-1. Laser irradiation at the power of 10 mW, 30 mW and 110 mW resulted in maximal response respectively reduced to 94%, 62% and 38%.ConclusionOur results strongly suggest that during low level laser irradiation vascular smooth muscle cells reactivity is reduced, this effect is present in arteries with normal endothelium. The mechanism of action of laser biosimulation on tissues is unclear.
      Authors of publications present the positive clinical impact of low level laser irradiation on vascular reactivity.
  • Trimethylamine-N-oxide, as a risk factor for atherosclerosis, induces
           stress in J774A.1 murine macrophages
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Abbas Mohammadi, Zakaria Vahabzadeh, Soran Jamalzadeh, Tahereh KhaliliAbstractPurposeTrimethylamine N-oxide (TMAO) is a biomarker for kidney problems. It has also been introduced as a risk factor for atherosclerosis. The classic risk factors for atherosclerosis trigger cellular and humeral immunoreaction in macrophages through induction of heat shock protein expressions and increased levels of GRP94 and HSP70 are associated with increased atherosclerosis risk. The present study evaluated the possible effect(s) of TMAO on the expression of GRP94 and HSP70 at protein levels.MethodsJ774A.1 murine macrophages were treated with different micromolar concentrations of TMAO and 4-phenylbutyric acid (PBA), a chemical chaperone, for 8, 18, 24, and 48 h intervals. Tunicamycin was also used as a control for induction of endoplasmic reticulum stress. Western blotting was used to evaluate the expression of GRP94 and HSP70 in macrophages at protein levels.ResultTunicamycin greatly increased protein levels of GRP94. Similarly, but to a lesser extent compared to tunicamycin, TMAO also increased GRP94. In 24 h treated cells, only 300 μM of TMAO, and in cells treated for 48 h, all doses of TMAO produced a significant increase in relative HSP70 protein levels compared to the control. PBA failed to induce any changes in GRP94 or HSP70 protein levels.ConclusionGRP94 and HSP70 are stress-inducible heat shock protein, so the elevation in J774A.1 murine macrophages can clearly define cells under stress and elucidate the contribution of stress induced by TMAO that may have a part in the abnormal activation of macrophages involved in foam cell formation.
  • Antidepressant-like activity of methyl jasmonate involves modulation of
           monoaminergic pathways in mice
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Solomon Umukoro, Adaeze Adebesin, Gladys Agu, Osarume Omorogbe, Stephen Babajide AsehindeAbstractPurposeThe efficacy of current antidepressant drugs has been compromised by adverse effects, low remission and delay onset of action necessitating the search for alternative agents. Methyl jasmonate (MJ), a bioactive compound isolated from Jasminum grandiflorum has been shown to demonstrate antidepressant activity but its mechanism of action remains unknown. Thus, the role of monoaminergic systems in the antidepression-like activity of MJ was investigated in this study.Materials and methodsMice were given i.p. injection of MJ (5, 10 and 20 mg/kg), imipramine (10 mg/kg) and vehicle (10 mL/kg) 30 min before the forced swim test (FST) and tail suspension test (TST) were carried out. The involvement of monoaminergic systems in the anti-depressant-like effect of MJ (20 mg/kg) was evaluated using p-chlorophenylalanine (pCPA), metergoline, yohimbine, prazosin, sulpiride and haloperidol in the TST.ResultsMJ significantly decrease the duration of immobility in the FST and TST relative to control suggesting antidepressant-like property. However, pretreatment with yohimbine (1 mg/kg, i.p., an α2-adrenergic receptor antagonist) or prazosin (62.5 μg/kg, i.p., an α1-adrenoceptor antagonist) attenuated the antidepressant-like activity of MJ. Also, pCPA; an inhibitor of serotonin biosynthesis (100 mg/kg, i.p) or metergoline (4 mg/kg, i.p., 5-HT2 receptor antagonist) reversed the anti-immobility effect of MJ. Sulpiride (50 mg/kg, i.p., a D2 receptor antagonist) or haloperidol (0.2 mg/kg, i.p., a dopamine receptor antagonist) reversed the anti-immobility effect of MJ.ConclusionThe results of this study suggest that serotonergic, noradrenergic and dopaminergic systems may play a role in the antidepressant-like activity of MJ.
  • Cytotoxic, genotoxic and antimicrobial activity of caffeic and rosmarinic
           acids and their lithium, sodium and potassium salts as potential
           anticancer compounds
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Marzena Matejczyk, Renata Świsłocka, Aleksandra Golonko, Włodzimierz Lewandowski, Eliza HawrylikPurposeThe aim of this study was to examine the cytotoxic, genotoxic, antioxidant and antimicrobial activity of caffeic and rosmarinic acids and their salts with Li, Na and K with use of Escherichia coli K-12 recA:gfp strain as a model organism.MethodsCytotoxic potency of tested chemicals were calculated on the basis on the dose that confers inhibition percentage such as 20% for each concentrations of analysed chemicals. Genotoxic properties were calculated on the basis of the fold increase (FI) of SFI values normalized with control. Antioxidant potencies were established on the base of DPPH assay. Antimicrobial activity of chemicals were established on the value of minimal inhibitory concentration (MIC).ResultsObtained results indicated that lower concentrations of tested compounds exhibited stronger GFP fluorescence response after rosmarinic acids and their salts treatment. Genotoxic effects seemed to be independent of the salt ions. The caffeic acid salts with Li, Na and K showed reduced genotoxic effect in comparison to the caffeic acid while increased cytotoxic effect than that of caffeic acid. Moreover, caffeinate salts exhibited better antimicrobial activity against E. coli (MIC = 250 μg/mL) than K caffeinate salt (MIC > 500 μg/mL). The MIC values of Li, Na and K rosmarinate salts were above 500 μg/mL against all tested microorganisms.ConclusionThe results of the experiment show that there is no clear positive correlation between the antioxidant potency of caffeic and rosmarinic acids and their Li, Na and K salts and their cytotoxic effect. Used salts ions Li, Na and K do not significantly affect the antioxidant effect of natural phenolic compounds and they do not have a significant impact on the biological parameters such as cyto- and genotoxicity. Perhaps it is connected with the reaction environment including polarity of the solvent (water).Graphical abstractGraphical abstract for this article
  • Genetic basis of enzymatic resistance of E. coli to
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Dominika Ojdana, Anna Sieńko, Paweł Sacha, Piotr Majewski, Piotr Wieczorek, Anna Wieczorek, Elżbieta TryniszewskaAbstractPurposeOver the past years, an increase in resistance to aminoglycosides has been observed among Enterobacteriaceae rods. This resistance development reduces therapeutic options for infections caused by multidrug-resistance organisms. Because of the changing epidemiology of extended-spectrum β-lactamases (ESBLs) and resistance to aminoglycosides, we investigated the prevalence of the aac(3)-Ia, aac(6′)-Ib, ant(4′)-IIa, ant(2”)-Ia, and aph(3”)-Ib genes encoding aminoglycoside-modifying enzymes (AMEs) in ESBL-producing Escherichia coli as well as ESBL-non-producing isolates. To understand bacterial resistance to aminoglycoside antibiotics, we estimated resistance phenotypes and the presence of genes responsible for this resistance.Materials and methodsThe study was conducted on 44 E.coli strains originated from patients hospitalized at University Hospital of Bialystok. MIC values were obtained for gentamicin, amikacin, netilmicin, and tobramycin. Isolates were tested for the presence of the aac(3)-Ia, aac(6′)-Ib, ant(4′)-IIa, ant(2”)-Ia, and aph(3”)-Ib genes with the use of the PCR technique.ResultsResistance to aminoglycosides was found in 79.5% of the isolates. The highest percentages of resistance were observed for tobramycin (70,5%) and gentamicin (59%), followed by netilmicin (43.2%) and amikacin (11.4%). PCR assays revealed the presence of aac(6′)-Ib among 26 (59.2%) strains, aph(3”)-Ib among 16 (36.2%), aac(3)-Ia among 7 (15.9%), and ant(2”)-Ia among 2 (4.6%) strains.ConclusionsThe enzymatic resistance against aminoglycosides in northeastern Poland among clinical isolates of E. coli is predominantly caused by aac(6′)-Ib and aph(3”)-Ib. Amikacin may be used for therapy of infections caused by ESBL-producing E. coli, because of the low rates of resistance.
  • Impact of intervention on metabolic outcomes among dropouts with type 2
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Merja K. Laine, Timo Kauppila, Mikko Honkasalo, Marko Raina, Johan G. ErikssonAbstractPurposeThe aim of this study was to evaluate the effect of an individual intervention given by health care professionals to dropouts with type 2 diabetes (T2D) on their metabolic profile.Materials/methodsIn 2010, we identified 356 T2D dropouts in Vantaa Health Centre, Finland. At the baseline visit the participants’ status was assessed including laboratory tests. Diabetes counseling was given, and drug treatment was enhanced when needed. The follow-up visit was performed 13 to 30 months later including the same assessments as performed at the baseline visit. The dropouts who attended the follow-up visit formed the study group. One third (n = 115) of the dropouts participated in the follow-up visit.ResultsThe study participants (mean age 61.4 years) were older than the non-participants (mean age 58.5 years) (p = 0.009). After the intervention the proportion of participants with hemoglobin A1c ≥ 9% (75 mmol/mol) decreased from 15.5% to 5.2% (p = 0.004). Improvements were also observed in general in hemoglobin A1c, from 6.6% (49 mmol/mol) to 6.3% (45 mmol/mol) (p = 0.001), in total cholesterol, from 4.9 mmol/l to 4.5 mmol/l (p = 0.011), in low-density lipoprotein cholesterol, from 2.9 mmol/l to 2.6 mmol/l (p = 0.015) and in diastolic blood pressure, from 90 mmHg to 84 mmHg (p = 0.001).ConclusionsDropouts with T2D were difficult to bring back to the public health care system, especially men under the age of 60 years. Dropouts who participated in the intervention showed improvements in several metabolic outcomes.
  • The alterations in alcohol dehydrogenase and aldehyde dehydrogenase
           activities in the sera of patients with renal cell carcinoma
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Karolina Orywal, Wojciech Jelski, Tadeusz Werel, Maciej SzmitkowskiAbstractPurposeIn a previous study we showed that the total activity of alcohol dehydrogenase (ADH) and its isoenzyme class I was significantly higher in renal cancer (RCC) cells compared to normal kidney. The aim of this study was to compare the activities of ADH isoenzymes and aldehyde dehydrogenase (ALDH) in the sera of patients with different stages of RCC and healthy subjects.Materials and methodsSerum samples were taken from 54 patients with clear cell RCC (17 patients in stage II, 22 in stage III and 15 in stage IV) and 52 healthy patients. Class III, IV of ADH and the total ADH activity was measured by the photometric method. For the measurement of ADH class I, II and the total ALDH activity we employed the fluorometric method.ResultsThe total activity of ADH and its isoenzyme class I were significantly higher in the sera of patients with every stage of RCC compared to healthy subjects. The analysis of ALDH activity did not indicate significant differences between tested groups.ConclusionsThe increased activity of total ADH and its isoenzyme class I in the sera of patients with RCC, seems to be caused by isoenzymes being released from cancerous cells and may be useful for diagnostics of renal cancer.
  • Limbic brain structures and burnout—A systematic review
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): YeeKong Chow, Jolanta Masiak, Emilia Mikołajewska, Dariusz Mikołajewski, Grzegorz Marcin Wójcik, Brian Wallace, Andy Eugene, Marcin OlajossyAbstractMore profound understanding of the relationship between the burnout and the limbic system function can provide better insight into brain structures associated with the burnout syndrome. The objective of this review is to explore all evidence of limbic brain structures associated with the burnout syndrome. In total, 13 studies were selected. Four of them applied the neuroimaging technology to investigate the sizes/volumes of the limbic brain structures of burnout patients. Six other studies were to investigate the hypothalamus-pituitary-adrenal (HPA) axis of burnout patients. Based on the results of the studies on the HPA-axis and neuroimaging of the limbic brain structures, one can see great impact of the chronic occupational stress on the limbic structures in terms of HPA dysregulation, a decrease of BDNF, impaired neurogenesis and limbic structures atrophy. It can be concluded that chronic stress inhibits the feedback control pathway in the HPA axis, causes the decrease of brain-derived neurotrophic factor (BDNF), then impaired neurogenesis and eventually neuron atrophy.
  • Human saliva as a diagnostic material
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Sylwia Chojnowska, Tomasz Baran, Iwona Wilińska, Paulina Sienicka, Iwona Cabaj-Wiater, Małgorzata KnaśAbstractToday blood biochemical laboratory tests are essential elements to the diagnosis and monitoring of the treatment of diseases. However, many researchers have suggested saliva as an preferable diagnostic material. The collection of saliva is simple, painless, cheap and safe, both for patients and medical staff. An additional advantage of saliva is the fact that it may be retrieved several times a day, which makes repeat analysis much easier. Furthermore, saliva has very high durability. Although 94–99% of salivary content is water, saliva also contains numerous cellular elements and many organic and inorganic substances, including most biological markers present in the blood and urine that may be used in the early detection and monitoring of many dental and general diseases.
  • Association of Brain-derived neurotrophic factor gene polymorphisms with
           body mass index: A systematic review and meta-analysis
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Shahab-Aldin Akbarian, Amin Salehi-Abargouei, Makan Pourmasoumi, Roya Kelishadi, Parvaneh Nikpour, Motahar Heidari-BeniAbstractBackgroundMany studies with inconsistent results have assessed the association of Brain-derived neurotrophic factor (BDNF) gene polymorphisms with prevalence of obesity and overweight. This review aims to provide a summary of the literature evaluating the relation between BDNF genotype and body mass index (BMI).MethodsA systematic search through PubMed, Scopus, Science direct, Ovid and Cochrane was performed. We included observational studies with cross-sectional and case-control design, which investigated relationship between all kinds of BDNF polymorphisms with BMI, as a representative index of obesity and overweight. Newcastle–Ottawa Scale was used to assess the quality of included articles.ResultsThirty five studies were included in quantitative synthesis. Analyses were performed separately using OR, β coefficient and mean. Significant association were documented between rs925946 and BMI (OR = 1.12, 95% CI = 1.08–1.17, P heterogeneity = 0.317), rs10501087 and BMI (OR = 1.14, 95% CI = 1.04–1.24, P heterogeneity = 0.861), rs6265 and BMI (OR = 1.13, 95% CI = 1.07–1.19, P heterogeneity = 0.406), rs988712 and BMI (OR = 1.29, 95% CI = 1.18–1.40, P heterogeneity = 0.602). According to pooled β coefficient analysis, significant result was only observed in the rs925946 polymorphism subgroup. Pooled mean analysis showed that overall effects for the association between BDNF polymorphisms and BMI were not statistically significant.ConclusionThis meta-analysis suggests that some polymorphisms in BDNF gene including rs925946, rs10501087, rs6265 and rs988712 can be considered as genetic determinants of obesity.
  • Patients with atrial fibrillation and coronary artery disease –
           Double trouble
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Ewelina Michniewicz, Elżbieta Mlodawska, Paulina Lopatowska, Anna Tomaszuk-Kazberuk, Jolanta MalyszkoAbstractCoronary artery disease (CAD) is the most common cardiovascular disease while atrial fibrillation (AF) is the most common cardiac arrhythmia. Both diseases share associated risk factors – hypertension, diabetes mellitus, sleep apnea, obesity and smoking. Moreover, inflammation plays a causative role in both diseases. The prevalence of CAD in patients with AF is from 17% to 46.5% while the prevalence of AF among patients with CAD is low and it is estimated from 0.2% to 5%. AF is a well-established factor of poor short- and long-term prognosis in patients with acute myocardial infarction (AMI) and is associated with a marked increase in overall mortality.The arrhythmia is common after cardiac surgeries and occurs in about 20 to 40% of patients after coronary artery bypass graft (CABG) surgery. It is predicted that between 5 and 15% of AF patients will require stenting at some point in their lives and will receive triple therapy with aspirin, clopidogrel or ticagrelor and oral anticoagulation (OAC). This requires careful consideration of antithrombotic therapy, balancing bleeding risk, stroke risk, and in-stent thrombosis with subsequent acute coronary syndromes. Co-prescription of OAC with antiplatelet therapy, in particular triple therapy, increases the absolute risk of major bleeding. In addition, major bleeding is associated with an up to 5-fold increased risk of death following an acute coronary syndrome. Coexistence of AF and CAD worsens the prognosis even in carefully treated patients.
  • A systematic review on the role of eicosanoid pathways in rheumatoid
    • Abstract: Publication date: March 2018Source: Advances in Medical Sciences, Volume 63, Issue 1Author(s): Malvina HoxhaAbstractBackgroundRheumatoid arthritis is characterized by the production of eicosanoids, cytokines, adhesion molecules, infiltration of T and B lymphocytes in the synovium and oxygen reduction accompanied by the cartilage degradation. Eicosanoids are responsible for the progressive destruction of cartilage and bone, however neither steroids, nor the non steroidal anti-inflammatory drugs (NSAIDs), cannot slow down cartilage and bone destruction providing only symptomatic improvement. The current rheumatoid arthritis treatment options include mainly the use of disease-modifying anti-rheumatic drugs, the corticosteroids, the NSAIDs and biological agents.MethodsPubMed, Cochrane, and Embase electronic database were used as the main sources for extracting several articles, reviews, original papers in English for further review and analysis on the implication of arachidonic acid metabolites with rheumatoid arthritis and different strategies of targeting arachidonic acid metabolites, different enzymes or receptors for improving the treatment of rheumatoid arthritis patients.ResultsWe first focused on the role of individual prostaglandins and leukotrienes, in the inflammatory process of arthritis, concluding with an outline of the current clinical situation of rheumatoid arthritis and novel treatment strategies targeting the arachidonic acid pathway.ConclusionsExtended research is necessary for the development of these novel compounds targeting the eicosanoid pathway, by increasing the levels of anti-inflammatory eicosanoids (PGD2,15dPGJ2), by inhibiting the production of pro-inflammatory eicosanoids (PGE2, LTB4, PGI2) involved in rheumatoid arthritis or also by developing dual compounds displaying both the COX-2 inhibitor/TP antagonist activity within a single compound.
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
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