Publisher: Springer-Verlag (Total: 2624 journals)

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Showing 1 - 200 of 2624 Journals sorted alphabetically
3D Printing in Medicine     Open Access   (Followers: 4)
3D Research     Hybrid Journal   (Followers: 21, SJR: 0.222, CiteScore: 1)
4OR: A Quarterly J. of Operations Research     Hybrid Journal   (Followers: 11, SJR: 0.825, CiteScore: 1)
AAPS J.     Hybrid Journal   (Followers: 30, SJR: 1.118, CiteScore: 4)
AAPS PharmSciTech     Hybrid Journal   (Followers: 9, SJR: 0.752, CiteScore: 3)
Abdominal Radiology     Hybrid Journal   (Followers: 20, SJR: 0.866, CiteScore: 2)
Abhandlungen aus dem Mathematischen Seminar der Universitat Hamburg     Hybrid Journal   (Followers: 4, SJR: 0.439, CiteScore: 0)
aBIOTECH : An Intl. J. on Plant Biotechnology and Agricultural Sciences     Hybrid Journal   (Followers: 1)
Academic Psychiatry     Full-text available via subscription   (Followers: 31, SJR: 0.53, CiteScore: 1)
Academic Questions     Hybrid Journal   (Followers: 9, SJR: 0.106, CiteScore: 0)
Accreditation and Quality Assurance: J. for Quality, Comparability and Reliability in Chemical Measurement     Hybrid Journal   (Followers: 33, SJR: 0.316, CiteScore: 1)
Acoustical Physics     Hybrid Journal   (Followers: 11, SJR: 0.359, CiteScore: 1)
Acoustics Australia     Hybrid Journal   (Followers: 1, SJR: 0.232, CiteScore: 1)
Acta Analytica     Hybrid Journal   (Followers: 7, SJR: 0.367, CiteScore: 0)
Acta Applicandae Mathematicae     Hybrid Journal   (Followers: 1, SJR: 0.675, CiteScore: 1)
Acta Biotheoretica     Hybrid Journal   (Followers: 3, SJR: 0.284, CiteScore: 1)
Acta Diabetologica     Hybrid Journal   (Followers: 19, SJR: 1.587, CiteScore: 3)
Acta Endoscopica     Hybrid Journal   (Followers: 1)
acta ethologica     Hybrid Journal   (Followers: 4, SJR: 0.769, CiteScore: 1)
Acta Geochimica     Hybrid Journal   (Followers: 7, SJR: 0.24, CiteScore: 1)
Acta Geodaetica et Geophysica     Hybrid Journal   (Followers: 3, SJR: 0.305, CiteScore: 1)
Acta Geophysica     Hybrid Journal   (Followers: 11, SJR: 0.312, CiteScore: 1)
Acta Geotechnica     Hybrid Journal   (Followers: 7, SJR: 1.588, CiteScore: 3)
Acta Informatica     Hybrid Journal   (Followers: 5, SJR: 0.517, CiteScore: 1)
Acta Mathematica     Hybrid Journal   (Followers: 12, SJR: 7.066, CiteScore: 3)
Acta Mathematica Hungarica     Hybrid Journal   (Followers: 2, SJR: 0.452, CiteScore: 1)
Acta Mathematica Sinica, English Series     Hybrid Journal   (Followers: 6, SJR: 0.379, CiteScore: 1)
Acta Mathematica Vietnamica     Hybrid Journal   (SJR: 0.27, CiteScore: 0)
Acta Mathematicae Applicatae Sinica, English Series     Hybrid Journal   (SJR: 0.208, CiteScore: 0)
Acta Mechanica     Hybrid Journal   (Followers: 25, SJR: 1.04, CiteScore: 2)
Acta Mechanica Sinica     Hybrid Journal   (Followers: 5, SJR: 0.607, CiteScore: 2)
Acta Metallurgica Sinica (English Letters)     Hybrid Journal   (Followers: 8, SJR: 0.576, CiteScore: 2)
Acta Meteorologica Sinica     Hybrid Journal   (Followers: 3, SJR: 0.638, CiteScore: 1)
Acta Neurochirurgica     Hybrid Journal   (Followers: 7, SJR: 0.822, CiteScore: 2)
Acta Neurologica Belgica     Hybrid Journal   (Followers: 3, SJR: 0.376, CiteScore: 1)
Acta Neuropathologica     Hybrid Journal   (Followers: 5, SJR: 7.589, CiteScore: 12)
Acta Oceanologica Sinica     Hybrid Journal   (Followers: 3, SJR: 0.334, CiteScore: 1)
Acta Physiologiae Plantarum     Hybrid Journal   (Followers: 4, SJR: 0.574, CiteScore: 2)
Acta Politica     Hybrid Journal   (Followers: 19, SJR: 0.605, CiteScore: 1)
Activitas Nervosa Superior     Hybrid Journal   (SJR: 0.147, CiteScore: 0)
Adaptive Human Behavior and Physiology     Hybrid Journal   (Followers: 1)
adhäsion KLEBEN & DICHTEN     Hybrid Journal   (Followers: 9, SJR: 0.103, CiteScore: 0)
ADHD Attention Deficit and Hyperactivity Disorders     Hybrid Journal   (Followers: 28, SJR: 0.72, CiteScore: 2)
Adhesion Adhesives & Sealants     Hybrid Journal   (Followers: 11)
Administration and Policy in Mental Health and Mental Health Services Research     Partially Free   (Followers: 21, SJR: 1.005, CiteScore: 2)
Adolescent Research Review     Hybrid Journal   (Followers: 2)
Adsorption     Hybrid Journal   (Followers: 5, SJR: 0.703, CiteScore: 2)
Advanced Composites and Hybrid Materials     Hybrid Journal  
Advanced Fiber Materials     Full-text available via subscription  
Advances in Applied Clifford Algebras     Hybrid Journal   (Followers: 4, SJR: 0.698, CiteScore: 1)
Advances in Astronautics Science and Technology     Hybrid Journal  
Advances in Atmospheric Sciences     Hybrid Journal   (Followers: 42, SJR: 0.956, CiteScore: 2)
Advances in Computational Mathematics     Hybrid Journal   (Followers: 23, SJR: 0.812, CiteScore: 1)
Advances in Contraception     Hybrid Journal   (Followers: 3)
Advances in Data Analysis and Classification     Hybrid Journal   (Followers: 57, SJR: 1.09, CiteScore: 1)
Advances in Gerontology     Partially Free   (Followers: 8, SJR: 0.144, CiteScore: 0)
Advances in Health Sciences Education     Hybrid Journal   (Followers: 35, SJR: 1.64, CiteScore: 2)
Advances in Manufacturing     Hybrid Journal   (Followers: 3, SJR: 0.475, CiteScore: 2)
Advances in Neurodevelopmental Disorders     Hybrid Journal  
Advances in Operator Theory     Hybrid Journal   (Followers: 2)
Advances in Polymer Science     Hybrid Journal   (Followers: 49, SJR: 1.04, CiteScore: 3)
Advances in Therapy     Hybrid Journal   (Followers: 5, SJR: 1.075, CiteScore: 3)
Advances in Traditional Medicine     Hybrid Journal   (Followers: 2)
Adversity and Resilience Science : J. of Research and Practice     Hybrid Journal   (Followers: 1)
Aegean Review of the Law of the Sea and Maritime Law     Hybrid Journal   (Followers: 8)
Aequationes Mathematicae     Hybrid Journal   (Followers: 2, SJR: 0.517, CiteScore: 1)
Aerobiologia     Hybrid Journal   (Followers: 4, SJR: 0.673, CiteScore: 2)
Aerosol Science and Engineering     Hybrid Journal  
Aerospace Systems     Hybrid Journal   (Followers: 1)
Aerotecnica Missili & Spazio : J. of Aerospace Science, Technologies & Systems     Hybrid Journal  
Aesthetic Plastic Surgery     Hybrid Journal   (Followers: 12, SJR: 0.825, CiteScore: 1)
Affective Science     Hybrid Journal   (Followers: 1)
African Archaeological Review     Hybrid Journal   (Followers: 20, SJR: 0.862, CiteScore: 1)
Afrika Matematika     Hybrid Journal   (Followers: 3, SJR: 0.235, CiteScore: 0)
Ageing Intl.     Hybrid Journal   (Followers: 7, SJR: 0.39, CiteScore: 1)
Aggiornamenti CIO     Hybrid Journal   (Followers: 1)
Aging Clinical and Experimental Research     Hybrid Journal   (Followers: 3, SJR: 0.67, CiteScore: 2)
Agricultural Research     Hybrid Journal   (Followers: 7, SJR: 0.276, CiteScore: 1)
Agriculture and Human Values     Open Access   (Followers: 15, SJR: 1.173, CiteScore: 3)
Agroforestry Systems     Open Access   (Followers: 20, SJR: 0.663, CiteScore: 1)
Agronomy for Sustainable Development     Open Access   (Followers: 18, SJR: 1.864, CiteScore: 6)
AI & Society     Hybrid Journal   (Followers: 9, SJR: 0.227, CiteScore: 1)
AIDS and Behavior     Hybrid Journal   (Followers: 17, SJR: 1.792, CiteScore: 3)
Air Quality, Atmosphere & Health     Hybrid Journal   (Followers: 6, SJR: 0.862, CiteScore: 3)
Akupunktur & Aurikulomedizin     Full-text available via subscription   (Followers: 1)
Algebra and Logic     Hybrid Journal   (Followers: 7, SJR: 0.531, CiteScore: 0)
Algebra Universalis     Hybrid Journal   (Followers: 2, SJR: 0.583, CiteScore: 1)
Algebras and Representation Theory     Hybrid Journal   (Followers: 1, SJR: 1.095, CiteScore: 1)
Algorithmica     Hybrid Journal   (Followers: 9, SJR: 0.56, CiteScore: 1)
Allergo J.     Full-text available via subscription   (Followers: 2, SJR: 0.234, CiteScore: 0)
Allergo J. Intl.     Hybrid Journal   (Followers: 2)
Alpine Botany     Hybrid Journal   (Followers: 5, SJR: 1.11, CiteScore: 3)
ALTEX : Alternatives to Animal Experimentation     Open Access   (Followers: 2)
AMBIO     Hybrid Journal   (Followers: 9, SJR: 1.569, CiteScore: 4)
American J. of Cardiovascular Drugs     Hybrid Journal   (Followers: 17, SJR: 0.951, CiteScore: 3)
American J. of Community Psychology     Hybrid Journal   (Followers: 31, SJR: 1.329, CiteScore: 2)
American J. of Criminal Justice     Hybrid Journal   (Followers: 9, SJR: 0.772, CiteScore: 1)
American J. of Cultural Sociology     Hybrid Journal   (Followers: 19, SJR: 0.46, CiteScore: 1)
American J. of Dance Therapy     Hybrid Journal   (Followers: 8, SJR: 0.181, CiteScore: 0)
American J. of Potato Research     Hybrid Journal   (Followers: 3, SJR: 0.611, CiteScore: 1)
American J. of Psychoanalysis     Hybrid Journal   (Followers: 22, SJR: 0.314, CiteScore: 0)
American Sociologist     Hybrid Journal   (Followers: 16, SJR: 0.35, CiteScore: 0)
Amino Acids     Hybrid Journal   (Followers: 7, SJR: 1.135, CiteScore: 3)
AMS Review     Partially Free   (Followers: 4)
Analog Integrated Circuits and Signal Processing     Hybrid Journal   (Followers: 11, SJR: 0.211, CiteScore: 1)
Analysis and Mathematical Physics     Hybrid Journal   (Followers: 6, SJR: 0.536, CiteScore: 1)
Analysis in Theory and Applications     Hybrid Journal   (Followers: 1)
Analysis of Verbal Behavior     Hybrid Journal   (Followers: 6)
Analytical and Bioanalytical Chemistry     Hybrid Journal   (Followers: 32, SJR: 0.978, CiteScore: 3)
Anatomical Science Intl.     Hybrid Journal   (Followers: 3, SJR: 0.367, CiteScore: 1)
Angewandte Schmerztherapie und Palliativmedizin     Hybrid Journal  
Angiogenesis     Hybrid Journal   (Followers: 3, SJR: 2.177, CiteScore: 5)
Animal Cognition     Hybrid Journal   (Followers: 23, SJR: 1.389, CiteScore: 3)
Annales françaises de médecine d'urgence     Hybrid Journal   (Followers: 1, SJR: 0.192, CiteScore: 0)
Annales Henri Poincaré     Hybrid Journal   (Followers: 3, SJR: 1.097, CiteScore: 2)
Annales mathématiques du Québec     Hybrid Journal   (Followers: 4, SJR: 0.438, CiteScore: 0)
Annali dell'Universita di Ferrara     Hybrid Journal   (SJR: 0.429, CiteScore: 0)
Annali di Matematica Pura ed Applicata     Hybrid Journal   (Followers: 1, SJR: 1.197, CiteScore: 1)
Annals of Biomedical Engineering     Hybrid Journal   (Followers: 19, SJR: 1.042, CiteScore: 3)
Annals of Combinatorics     Hybrid Journal   (Followers: 4, SJR: 0.932, CiteScore: 1)
Annals of Data Science     Hybrid Journal   (Followers: 13)
Annals of Dyslexia     Hybrid Journal   (Followers: 11, SJR: 0.85, CiteScore: 2)
Annals of Finance     Hybrid Journal   (Followers: 36, SJR: 0.579, CiteScore: 1)
Annals of Forest Science     Hybrid Journal   (Followers: 7, SJR: 0.986, CiteScore: 2)
Annals of Functional Analysis     Hybrid Journal   (Followers: 1)
Annals of Global Analysis and Geometry     Hybrid Journal   (Followers: 1, SJR: 1.228, CiteScore: 1)
Annals of Hematology     Hybrid Journal   (Followers: 15, SJR: 1.043, CiteScore: 2)
Annals of Mathematics and Artificial Intelligence     Hybrid Journal   (Followers: 14, SJR: 0.413, CiteScore: 1)
Annals of Microbiology     Hybrid Journal   (Followers: 13, SJR: 0.479, CiteScore: 2)
Annals of Nuclear Medicine     Hybrid Journal   (Followers: 4, SJR: 0.687, CiteScore: 2)
Annals of Operations Research     Hybrid Journal   (Followers: 11, SJR: 0.943, CiteScore: 2)
Annals of Ophthalmology     Hybrid Journal   (Followers: 14)
Annals of PDE     Hybrid Journal  
Annals of Regional Science     Hybrid Journal   (Followers: 9, SJR: 0.614, CiteScore: 1)
Annals of Software Engineering     Hybrid Journal   (Followers: 13)
Annals of Solid and Structural Mechanics     Hybrid Journal   (Followers: 11, SJR: 0.239, CiteScore: 1)
Annals of Surgical Oncology     Hybrid Journal   (Followers: 18, SJR: 1.986, CiteScore: 4)
Annals of Telecommunications     Hybrid Journal   (Followers: 9, SJR: 0.223, CiteScore: 1)
Annals of the Institute of Statistical Mathematics     Hybrid Journal   (Followers: 1, SJR: 1.495, CiteScore: 1)
Antonie van Leeuwenhoek     Hybrid Journal   (Followers: 5, SJR: 0.834, CiteScore: 2)
Apidologie     Hybrid Journal   (Followers: 5, SJR: 1.22, CiteScore: 3)
APOPTOSIS     Hybrid Journal   (Followers: 9, SJR: 1.424, CiteScore: 4)
Applicable Algebra in Engineering, Communication and Computing     Hybrid Journal   (Followers: 3, SJR: 0.294, CiteScore: 1)
Applications of Mathematics     Hybrid Journal   (Followers: 3, SJR: 0.602, CiteScore: 1)
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 42, SJR: 0.571, CiteScore: 2)
Applied Biochemistry and Microbiology     Hybrid Journal   (Followers: 19, SJR: 0.21, CiteScore: 1)
Applied Categorical Structures     Hybrid Journal   (Followers: 4, SJR: 0.49, CiteScore: 0)
Applied Composite Materials     Hybrid Journal   (Followers: 53, SJR: 0.58, CiteScore: 2)
Applied Entomology and Zoology     Partially Free   (Followers: 7, SJR: 0.422, CiteScore: 1)
Applied Geomatics     Hybrid Journal   (Followers: 3, SJR: 0.733, CiteScore: 3)
Applied Geophysics     Hybrid Journal   (Followers: 10, SJR: 0.488, CiteScore: 1)
Applied Intelligence     Hybrid Journal   (Followers: 15, SJR: 0.6, CiteScore: 2)
Applied Magnetic Resonance     Hybrid Journal   (Followers: 4, SJR: 0.319, CiteScore: 1)
Applied Mathematics & Optimization     Hybrid Journal   (Followers: 10, SJR: 0.886, CiteScore: 1)
Applied Mathematics - A J. of Chinese Universities     Hybrid Journal   (Followers: 1, SJR: 0.17, CiteScore: 0)
Applied Mathematics and Mechanics     Hybrid Journal   (Followers: 5, SJR: 0.461, CiteScore: 1)
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 68, SJR: 1.182, CiteScore: 4)
Applied Physics A     Hybrid Journal   (Followers: 10, SJR: 0.481, CiteScore: 2)
Applied Physics B: Lasers and Optics     Hybrid Journal   (Followers: 27, SJR: 0.74, CiteScore: 2)
Applied Psychophysiology and Biofeedback     Hybrid Journal   (Followers: 8, SJR: 0.519, CiteScore: 2)
Applied Research in Quality of Life     Hybrid Journal   (Followers: 12, SJR: 0.316, CiteScore: 1)
Applied Solar Energy     Hybrid Journal   (Followers: 22, SJR: 0.225, CiteScore: 0)
Applied Spatial Analysis and Policy     Hybrid Journal   (Followers: 6, SJR: 0.542, CiteScore: 1)
Aquaculture Intl.     Hybrid Journal   (Followers: 26, SJR: 0.591, CiteScore: 2)
Aquarium Sciences and Conservation     Hybrid Journal   (Followers: 2)
Aquatic Ecology     Hybrid Journal   (Followers: 38, SJR: 0.656, CiteScore: 2)
Aquatic Geochemistry     Hybrid Journal   (Followers: 3, SJR: 0.591, CiteScore: 1)
Aquatic Sciences     Hybrid Journal   (Followers: 14, SJR: 1.109, CiteScore: 3)
Arabian J. for Science and Engineering     Hybrid Journal   (Followers: 5, SJR: 0.303, CiteScore: 1)
Arabian J. of Geosciences     Hybrid Journal   (Followers: 2, SJR: 0.319, CiteScore: 1)
Archaeological and Anthropological Sciences     Hybrid Journal   (Followers: 23, SJR: 1.052, CiteScore: 2)
Archaeologies     Hybrid Journal   (Followers: 13, SJR: 0.224, CiteScore: 0)
Archiv der Mathematik     Hybrid Journal   (Followers: 1, SJR: 0.725, CiteScore: 1)
Archival Science     Hybrid Journal   (Followers: 70, SJR: 0.745, CiteScore: 2)
Archive for History of Exact Sciences     Hybrid Journal   (Followers: 7, SJR: 0.186, CiteScore: 1)
Archive for Mathematical Logic     Hybrid Journal   (Followers: 3, SJR: 0.909, CiteScore: 1)
Archive for Rational Mechanics and Analysis     Hybrid Journal   (SJR: 3.93, CiteScore: 3)
Archive of Applied Mechanics     Hybrid Journal   (Followers: 6, SJR: 0.79, CiteScore: 2)
Archives and Museum Informatics     Hybrid Journal   (Followers: 177, SJR: 0.101, CiteScore: 0)
Archives of Computational Methods in Engineering     Hybrid Journal   (Followers: 6, SJR: 1.41, CiteScore: 5)
Archives of Dermatological Research     Hybrid Journal   (Followers: 7, SJR: 1.006, CiteScore: 2)
Archives of Environmental Contamination and Toxicology     Hybrid Journal   (Followers: 14, SJR: 0.773, CiteScore: 2)
Archives of Gynecology and Obstetrics     Hybrid Journal   (Followers: 19, SJR: 0.956, CiteScore: 2)
Archives of Microbiology     Hybrid Journal   (Followers: 10, SJR: 0.644, CiteScore: 2)
Archives of Orthopaedic and Trauma Surgery     Hybrid Journal   (Followers: 10, SJR: 1.146, CiteScore: 2)
Archives of Osteoporosis     Hybrid Journal   (Followers: 2, SJR: 0.71, CiteScore: 2)
Archives of Sexual Behavior     Hybrid Journal   (Followers: 11, SJR: 1.493, CiteScore: 3)
Archives of Toxicology     Hybrid Journal   (Followers: 18, SJR: 1.541, CiteScore: 5)
Archives of Virology     Hybrid Journal   (Followers: 5, SJR: 0.973, CiteScore: 2)
Archives of Women's Mental Health     Hybrid Journal   (Followers: 18, SJR: 1.274, CiteScore: 3)
Archivio di Ortopedia e Reumatologia     Hybrid Journal  
Archivum Immunologiae et Therapiae Experimentalis     Hybrid Journal   (Followers: 2, SJR: 0.946, CiteScore: 3)
ArgoSpine News & J.     Hybrid Journal  
Argumentation     Hybrid Journal   (Followers: 6, SJR: 0.349, CiteScore: 1)
Arid Ecosystems     Hybrid Journal   (Followers: 3, SJR: 0.2, CiteScore: 0)
Arkiv för Matematik     Hybrid Journal   (Followers: 1, SJR: 0.766, CiteScore: 1)
arktos : The J. of Arctic Geosciences     Hybrid Journal  
Arnold Mathematical J.     Hybrid Journal   (Followers: 1, SJR: 0.355, CiteScore: 0)
Arthropod-Plant Interactions     Hybrid Journal   (Followers: 2, SJR: 0.839, CiteScore: 2)
Arthroskopie     Hybrid Journal   (Followers: 1, SJR: 0.131, CiteScore: 0)

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Similar Journals
Journal Cover
Applied Biochemistry and Microbiology
Journal Prestige (SJR): 0.21
Citation Impact (citeScore): 1
Number of Followers: 19  
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1608-3024 - ISSN (Online) 0003-6838
Published by Springer-Verlag Homepage  [2624 journals]
  • Conformation of Polymer-Carrier Macromolecules and the Activity of
           Immobilized Enzyme
    • Abstract: The dependence of the activity of immobilized trypsin on the conformation of the polymer carrier macromolecule was studied. Fluorescence spectroscopy shows that immobilization of the enzyme on a rigid polymer chain prevents the association of enzyme molecules and ensures the availability of its active centers, even for high-molecular-weight molecules.
      PubDate: 2020-01-01
  • Determination of the Mycelium and Antigens of a Number of Micromycetes in
           Soil Extracts via Enzyme-Linked Immunosorbent Assay
    • Abstract: Sixteen variants of test systems (two-site sandwich and competitive enzyme-linked immunosorbent assay (ELISA)) were studied to determine the concentration of microfungi, some of which are known opportunistic pathogens for humans, in soil extracts. The correlation of the amount of fungal biomass (the length of the growing mycelium) with the concentrations of antigens detected by the two-site sandwich ELISA method and the competitive ELISA method was evaluated. It has been shown that the methods are highly effective in the case of long incubation (more than 5 days) of soil samples and are ineffective for short-term incubation, because there was no correlation between hypha length and antigen concentration during the first day of cultivation. The perspectivity of ELISA methods for the detection of some soil microfungi from genera Aspergillus (Asp. flavus, Asp. fumigatus, Asp. niger), Fusarium (F. solani, F. poae), and others (Alternaria alternata, Phoma lingham, Mucor hiemalis) was shown. The competitive ELISA method was more efficient than two-site sandwich ELISA.
      PubDate: 2020-01-01
  • Ommochromes from Hermetia illucens : Isolation and Study of Antioxidant
           Characteristics and Antimicrobial Activity
    • Abstract: A technique for the isolation and purification of ommochromes (pigments of the complex eye of the adult Hermetia illucens fly) has been developed. Isolated ommochromes showed fluorescence with emission maxima at 460–470 nm and 525–535 nm, the intensity of which increased abruptly during their oxidation with hydrogen peroxide. The method of chemiluminescence quenching of luminol was used to determine the antiradical activity of ommochromes; the constant value was (1.2 ± 0.5) × 104 M–1. A finely dispersed mixture of ommochromes in 0.1 M potassium phosphate buffer (pH 7.4) containing bovine serum albumin (2.0 mg/mL) showed antimicrobial activity against B. subtilis ATCC 6633, Candida albicans ATCC 2091, and Aspergillus niger INA 00760. The H. illucens imago was concluded to be a promising renewable source of natural pigments with good antioxidant characteristics and antibacterial properties.
      PubDate: 2020-01-01
  • The Screening of Refolding Conditions and Obtainment of the Recombinant
           Antistaphylococcal Endolysin LysK CA in Active Form from E. coli Inclusion
    • Abstract: Step-by-step screening of the main characteristics of refolding buffer is proposed to obtain recombinant antistaphylococcal endolysin LysK containing two catalytic domains, CHAP and amidase-2, in active form from E. coli inclusion bodies. The optimal pH, temperature, redox potential of the refolding buffer, as well as the optimal protein concentration and type of antiaggregation compound were determined. The composition of a renaturation system of antistaphylococcal endolysin containing 20 mM phosphate buffer, pH 7.4, 0.4 M sucrose, and 2.5 mM DTT at 10°C with dilution to a final concentration of ~150 μg/mL was found to be optimal. The refolding yield after scaling was about 29.5 ± 6.7%, which produced 16 ± 2.3 mg of the target protein from 2.25 g of washed inclusion bodies with a specific enzyme activity of 1.8 ± 0.73 × 103 U/mg.
      PubDate: 2020-01-01
  • Effect of Cultivation Conditions on Poly(3 - hydroxybutyrate) Synthesis by
           Nodule Bacteria Rhizobium phaseoli
    • Abstract: The synthesis of poly(3-hydroxybutyric acid) (PHB) in nodule bacteria Rhizobium phaseoli grown in submerged culture was studied. The Law method for the determination of PHB was modified, which made it possible to isolate the polymer and to carry out its quantitative assessment directly in the biomass. Only S forms of R. phaseoli cells could synthesize PHB. Its accumulation was more active on carbon-rich medium (by ~50%), and the polymer granules were more clearly visible. The PHB synthesis increased with a decrease in aeration, and the maximum content was reached after 35−40 h of growth. The effect of the oxidative stress caused by t-butyl hydroperoxide and benzyl viologen on the growth of PHB-synthesizing bacteria was studied. Both compounds negatively affected bacterial growth. The growth was reactivated only in PHB-synthesizing S forms. We can conclude that PHB is a protector for nodule bacteria under the conditions of oxidative stress. The maximal PHB content in R. phaseoli cells reached 54% of the dry cell weight, which exceeded the values previously described for rhizobia. This value is close to those reported for the bacterial strains producing the polymer and used in biotechnology. We can conclude that rhizobia grown in the submerged culture can be potential producers of PHB and other polyhydroxyalkanoates.
      PubDate: 2020-01-01
  • Purification of the Protease Activator of Protein C of Human Blood Plasma
           Produced by the Micromycete Aspergillus ochraceus VKM F-4104D
    • Abstract: A method has been developed for the purification of a protease activator of protein C in human blood plasma from the culture fluid of micromycete Aspergillus ochraceus VKM F-4104D. The properties of this protein are similar to those of the protein-C activator from the venom of the snake Agkistrodon contortrix contortrix, which is used in modern laboratory diagnostics of protein C. The method combines fractionation by ammonium sulfate and hydrophobic, ion-exchange, and gel-permeation chromatography. As a result, a highly purified preparation of protein-C activator was obtained with a 350-fold increase in specific activity during the purification process.
      PubDate: 2020-01-01
  • Role of Phosphate-Accumulating Bacteria in Biological Phosphorus Removal
           from Wastewater
    • Abstract: The review examines the microbiological aspects of the biological phosphorus removal from wastewater. The history of the development of biotechnology and the discovery of the physiological group of phosphate-accumulating organisms (PAOs), which biologically remove phosphorus via the phosphate uptake and storage in the form of intracellular polyphosphates, is briefly described. PAOs are characterized by a cyclic type of metabolism that occurs when the anaerobic/aerobic conditions cyclically change. Under anaerobic conditions, PAOs uptake and store organic compounds through the energy of degradation of intracellular polyphosphates. When anaerobic conditions change to aerobic or an alternative electron acceptor appears, PAOs uptake phosphates and synthesize intracellular polyphosphates using the intracellular polymeric sources of carbon and energy accumulated under anaerobic conditions. The main representatives of the PAOs, their metabolic models, and physiological characteristics are described. The basic principles of the implementation of biotechnology used in the practice of wastewater treatment for phosphorus and other nutrients are considered.
      PubDate: 2020-01-01
  • Electro-Optical Detection of Phage Antibody Interaction with Complementary
           Antigens of Herbaspirillum seropedicae Z78 Cells
    • Abstract: Complementary interactions in the antigen–antibody system have been assessed for the first time via electrooptical analysis with phage antibodies to the main antigens of the Herbaspirillum seropedicae Z78. It is shown that an electrooptical analyzer makes it possible to distinguish the presence or absence of specific interactions of phage antibodies with the main epitopes of a bacterial surface. The revealed relationships in the electrophysical parameters are in good agreement with the component composition of Herbaspirillum antigens and their topographic distribution, and they were also confirmed with electron microscopy and dot-analysis. It is shown that electrooptical analysis can be used to detect Herbaspirillum spp. with exposed antigens on its surface. The results can serve as a basis for the development of an express test for the rapid identification of these microorganisms.
      PubDate: 2020-01-01
  • Prospects and Applications of Lipopeptide-Producing Bacteria for Plant
           Protection (Review)
    • Abstract: The review provides current information on bacterial lipopeptides, mainly those produced by representatives of the genus Bacillus, which play an important role in plant protection against fungi, bacteria, and viruses exhibiting fungicidal, antibacterial, antiviral, and insecticidal properties, as well as phytoimmuno-modulating activity. The present review highlights the characteristics of bacterial lipopeptides, the genes responsible for their production, isolation methods from bacteria and estimation methods (quantitative and qualitative). The plant-protective mechanisms in reaction to lipopeptides, which are associated with signaling systems regulated by salicylates and jasmonates, are discussed. The review confirms that the search for rhizosphere and endophytic strains are promising for the creation of biological products that protect plants from a complex pathogens and pests and stimulate crop growth and productivity.
      PubDate: 2020-01-01
  • Coumarin Amine-Reactive DYE C392STP: an Efficient Building Block to
           Synthesize Single Labeled Oligonucleotides with Application as Fish Probes
    • Abstract: The demand of new reactive fluorophores with improved photophysical characteristics for labeling oligonucleotide probes is growing. Sodium (E/Z)-4-(4-(2-(6,7-dimethoxycoumarin-3-yl)vinyl)-benzoyl)-2,3,5,6-tetrafluoro-benzenesulfonate (C392STP) is an amine-reactive fluorescent dye that has recently shown interesting photophysical properties. In this work, we investigate the possibility of: i) synthesizing it cheaply and easily; ii) using this reactive dye to synthesize single-labeled fluorescent oligonucleotides; and iii) applying the resulting fluorescently labeled oligonucleotides as fluorescence in situ hybridization (FISH) probes. According to the results obtained in this study, C392STP: i) can be synthesized through a low cost and simple pathway; ii) is an efficient building block for mono-labeling of oligomers with an efficiency as high as that of an amine-reactive Alexa Fluor® 488 dye supplied in a commercial oligonucleotide labeling kit. Furthermore, the RNA-FISH assays revealed that C392-mono-labeled probes containing standard carbon linkers (C6) exhibited effective performance as RNA-FISH probes. These findings evidenced the applicability of C392STP in labeling of biomolecules and bioimaging. The results invite the researchers to continue exploring other uses of this reactive dye for exploiting its potential in biological applications.
      PubDate: 2020-01-01
  • Production of Human Papillomavirus Type-16 L1 VLP in Pichia pastoris
    • Abstract: Human papillomavirus-16 (HPV-16) is primary etiological agent of invasive cervical cancer development in the world population. There is no treatment for HPV infection. As a possible tool for prophylactic vaccination, the development of virus-like particles (VLPs) comprising the HPV-16 L1 capsid protein is highly preferred. The expression of HPV-16 L1 gene was carried out in Pichia pastoris. It was done as a promising vaccine candidate against HVP-16 infection for developing countries. The codon optimization for HPV-16 L1 gene was done and synthesized in pPICZA plasmid. The expression of HPV-16 L1 was evaluated in P. pastoris after induction with methanol. The purification of HPV-16 VLPs was accomplished by the ultra-centrifugation using the sucrose density gradient. The sera of 101 subjects including 16 patients positive for HPV-16 and 85 individuals negative for HPV-16 were tested by ELISA assay using anti-HPV-16 antibodies. The results of ELISA test have depicted no false positive and no false negative as compared with commercial ELISA kit. The results of HPV-16 L1 expression in P. pastoris showed a band of about 56 kDa by SDS-PAGE, and were confirmed by Western-blot assay. The formation of VLP and the self-assembly of HPV-16 L1 major capsid protein in VLP was observed by transmission electron and atomic force microscopies.
      PubDate: 2020-01-01
  • Cloning of Nucleoside Phosphorylase Genes from the Extremophilic Bacterium
           Halomonas chromatireducens AGD 8-3 with the Construction of Recombinant
           Producer Strains of These Proteins and the Study of Their Enzymatic
    • Abstract: Thymidine phosphorylase (deoA) and purine nucleoside phosphorylase (deoD) genes from the extremophilic bacterium Halomonas chromatireducens AGD 8-3 have been cloned. Expression plasmids were constructed, and highly efficient recombinant producer strains were obtained for these proteins. Recombinant nucleoside phosphorylases were isolated by ion-exchange chromatography in a homogeneous state, and their physical and enzymatic properties were studied. It was shown that the studied thymidine phosphorylase (HrTPP) and purine nucleoside phosphorylase (HrPNP) form the dimeric and hexameric forms, respectively. It was shown that the specific activity of HrTPP from the extremophilic bacterium H. chromatireducens AGD 8-3 was higher relative to thymidine (in comparison with its counterpart from E. coli).
      PubDate: 2020-01-01
  • Effects of Substrate and Cultivation Duration on the Productivity,
           Biological Activity, and Chromatography Profiles of Extracts Obtained from
           Stagonospora cirsii S-47
    • Abstract: In order to determine the possible toxic properties of a potential mycoherbicide based on the fungus Stagonospora cirsii S-47, the effects of solid and liquid media composition on the yield of extractive matter (YEM), the spectrum of biological activity, and the chromatography profiles of extracts obtained from cultures of this fungus were studied. The maximal YEM (~250 mg/L) was obtained from filtrates taken from a 2-week culture of S. cirsii S-47 grown on a sucrose–soy meal medium. The same time was optimal for the production of maximal YEM (~1.5 g/kg) when the fungus was grown on pearl-barley substrate. The fungal extracts showed phytotoxic, antimicrobial, cytotoxic, and antiesterase activity. Extracts from culture filtrates generally demonstrated a higher level and wider spectrum of biological activity than extracts from solid S. cirsii S-47 culture. The maximal phytotoxic and antimicrobial activity was found in dichloromethane extracts from the filtrate of 3-week fungal culture on Czapek medium, while extracts from two-week-old culture strongly inhibited carboxyl esterase. The maximal cytotoxic activity against the U251 cell line was found in ethyl acetate extract from a 3-week culture of S. cirsii S-47 produced on YMG liquid medium. The extract compositions varied considerably depending on the culture substrate.
      PubDate: 2020-01-01
  • Preparation and Properties of Immobilized Particles Containing Highly
           Efficient Nitrogen-Fixing Klebsiella variicola GN02 Cells Isolated from
           the Pennisetum giganteum z. x. lin Roots
    • Abstract: The biological properties, structural characterization, and performance of Klebsiella variicola GN02 isolated from the roots of P. sinense and embedded in sodium alginate supplemented with 10% sterile skim milk were investigated. The results showed that the immobilized K. variicola GN02 particles were more stable and had a higher survival rate than free bacteria. Infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis showed that immobilized K. variicola GN02 particles had the peaks associated with sodium alginate and K. variicola GN02 cells, indicating that K. variicola GN02 cells were successfully embedded in the sodium alginate. Compared with free bacteria, the immobilized K. variicola GN02 cells revealed slower but a longer growth period during the 40 h culture period. The OD600 and ammonia secretion of immobilized K. variicola GN02 particles were 0.87 and 18.45 mg/L, respectively. A solution incubated with immobilized K. variicola GN02 exhibited a growth-promoting effect on corn than free K. variicola GN02. Root length, plant height, fresh weight, and total nitrogen content of plants subjected to immobilized K. variicola GN02 increased by 15.3, 16.3, 8.9, and 32.3%, respectively. The number of viable K. variicola GN02 in soil also increased significantly to 20.86 × 105 CFU/g. Therefore, immobilized K. variicola GN02 has promising application value, and this study provides a theoretical basis and reference for developing a biological fertilizer with new nitrogen-fixing bacteria.
      PubDate: 2020-01-01
  • Ubiquitin-Specific E. coli Proteinase Does Not Require the Obligatory
           Presence of Dipeptide GlyGly at Processing Site
    • Abstract: It is shown that, unlike eukaryotic ubiquitin-specific proteinases (deubiquitinating enzymes, DUBs), the E. coli deubiquitinase ElaD can process sites containing not only GlyGly but also GlyAla with equal efficiency. At the same time, the bacterial enzyme, like eukaryotic DUBs, fails to process sites containing AlaGly, AlaAla, or GlyPro dipeptides. In light of the functional mission of the E. coli deubiquitinating agent, its decreased specificity can be considered a valuable evolutional acquisition that allows expansion of the list of molecular targets attacked during pathogenesis.
      PubDate: 2019-12-01
  • Increased Isoprene Production by the Recombinant Pantoea ananatis Strain
           due to the Balanced Amplification of Mevalonate Pathway Genes
    • Abstract: The uncoordinated expression of a target biosynthetic pathway often results in low growth and productivity for a producing strain due to the accumulation of toxic metabolic intermediates. In this study, we report an increase in isoprene accumulation by an engineered strain of Pantoea ananatis that possesses foreign, artificial mevalonate-pathway genes. This was achieved due to the simultaneous, φ80-Int-dependent integration of a mixture of conditionally replicated plasmids carrying genes for the upper and lower mevalonate pathways and mevalonate kinase, followed by selection according to the isoprene accumulation of clones with optimal copy numbers of the integrative cassettes. A previously developed method, Dual In/Out, was modified to accelerate the construction of recipient strains containing several attB sites of the φ80 phage in their genomes.
      PubDate: 2019-12-01
  • Study of the Strain Bacillus pumilus B-13176 and Its Metabolites with
           Fungicidal and Antibacterial Activities against Aspergillus niger and
           Staphylococcus aureus (MRSA)
    • Abstract: Strain B-13176, which manifests antibacterial and fungicidal activity, has been isolated via the screening of spore-forming bacteria and identified as Bacillus pumilus based on an analysis of variable regions of 16S RNA sequences. The cultural and morphological characteristics of the strain were studied, the dynamics of the synthesis of fungicidal and antibacterial metabolites and their location in culture were determined, and their resistance to physicochemical factors was investigated. It was shown that the strain possesses a pronounced activity against Aspergillus niger fungi and methycillin-resistant Staphylococcus aureus (MRSA) bacteria. The culture liquid of the strain was fractioned via centrifugation, ultrafiltration, extraction, and high-performance liquid chromatography (HPLC). Mass-spectrometry was used to show that the Bacillus pumilus B-13176 strain produces active, thermostable, protease-resistant metabolites of peptide origin that are located in the CL pellet (the fungicidal component) and supernatant (the antibacterial component).
      PubDate: 2019-12-01
  • Genome Editing: Current State of Research and Application to Animal
    • Abstract: The creation and development of genome editing (GE) technologies bring new opportunities for the genetic engineering of farm mammals and poultry. The present review characterizes GE systems based on ZFN, TALEN, and CRISPR/Cas9 and directions for their improvement in relation to farm animals. The fields for the application of GE technologies in animal husbandry and poultry farming and the objectives and prospects for their further development are discussed.
      PubDate: 2019-12-01
  • Expression of the Xylanase Gene from Paenibacillus brasilensis X1 in
           Pichia pastoris and Characteristics of the Recombinant Enzyme
    • Abstract: The heterologous expression, isolation, and characterization of a novel xylanase from Paenibacillus brasilensis are described. The xyl1 gene from the Paenibacillus brasilensis strain X1 VKPM B-13092, which consists of 639 nucleotides, encodes a secreted endo-1,4-β-xylanase (EC containing 184 amino acids and 28 residues of the putative signal peptide in the N-terminal region. The nucleotide sequence of the xyl1 gene and the amino acid sequence of the mature Xyll protein have the greatest homology with the Bacillus subtilis endo-1,4-β-xylanase sequences (78 and 83%, respectively). A gene fragment encoding the mature protein was expressed in Pichia pastoris. The purified recombinant Xyl1 enzyme was able to use birch xylan and arabinoxylan as substrates. With birch xylan, the optimal pH for the enzymatic reaction was 6.0, the optimal temperature was 40–50°C, and Km and Vmax, were equal to 1.1288 mg/mL and 5124.3 μmol/(min mg), respectively. The recombinant Xyl1 protein showed high pH and thermal stability, and the resistance to digestive enzymes and xylanase protein inhibitors from cereals. It was also shown that Mn2+ and Со2+ ions stimulate enzyme activity.
      PubDate: 2019-12-01
  • Biological Preparations for the Protection of Agricultural Plants (Review)
    • Abstract: The protection of plants from diseases caused by various pathogens is an economically and socially important problem; the losses in crop production constitute 20% of the harvest in different parts of the world. The use of chemical pesticides is the main method of plant protection. However, chemical preparations have a number of serious disadvantages. Biologicals for plant protection (BPP) are currently being developed more intensely. The world biggest chemical companies, BASF, Bayer and Syngenta, show great interest in the market for preparations of biological control. The data on markets of biologicals for the protection of agricultural plants in various parts of the world (North America, Europe, China, Latin America and Russia) are presented. According to expert data, the biological markets in these areas (with the exception of Russia) will exceed $1 billion by 2025. In Russia, only 0.3% of agricultural land is treated with biological products. The review contains data on the use of biofungicides obtained at GosNIIgenetika for the protection of wheat, potato, and vegetable crops. A high efficiency of the biofungicides has been demonstrated.
      PubDate: 2019-12-01
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
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