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Publisher: Springer-Verlag (Total: 2573 journals)

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Showing 1 - 200 of 2573 Journals sorted alphabetically
3D Printing in Medicine     Open Access   (Followers: 4)
3D Research     Hybrid Journal   (Followers: 21, SJR: 0.222, CiteScore: 1)
4OR: A Quarterly J. of Operations Research     Hybrid Journal   (Followers: 11, SJR: 0.825, CiteScore: 1)
AAPS J.     Hybrid Journal   (Followers: 29, SJR: 1.118, CiteScore: 4)
AAPS PharmSciTech     Hybrid Journal   (Followers: 8, SJR: 0.752, CiteScore: 3)
Abdominal Radiology     Hybrid Journal   (Followers: 18, SJR: 0.866, CiteScore: 2)
Abhandlungen aus dem Mathematischen Seminar der Universitat Hamburg     Hybrid Journal   (Followers: 4, SJR: 0.439, CiteScore: 0)
Academic Psychiatry     Full-text available via subscription   (Followers: 30, SJR: 0.53, CiteScore: 1)
Academic Questions     Hybrid Journal   (Followers: 9, SJR: 0.106, CiteScore: 0)
Accreditation and Quality Assurance: J. for Quality, Comparability and Reliability in Chemical Measurement     Hybrid Journal   (Followers: 31, SJR: 0.316, CiteScore: 1)
Acoustical Physics     Hybrid Journal   (Followers: 11, SJR: 0.359, CiteScore: 1)
Acoustics Australia     Hybrid Journal   (Followers: 1, SJR: 0.232, CiteScore: 1)
Acta Analytica     Hybrid Journal   (Followers: 7, SJR: 0.367, CiteScore: 0)
Acta Applicandae Mathematicae     Hybrid Journal   (Followers: 1, SJR: 0.675, CiteScore: 1)
Acta Biotheoretica     Hybrid Journal   (Followers: 4, SJR: 0.284, CiteScore: 1)
Acta Diabetologica     Hybrid Journal   (Followers: 19, SJR: 1.587, CiteScore: 3)
Acta Endoscopica     Hybrid Journal   (Followers: 1)
acta ethologica     Hybrid Journal   (Followers: 4, SJR: 0.769, CiteScore: 1)
Acta Geochimica     Hybrid Journal   (Followers: 7, SJR: 0.24, CiteScore: 1)
Acta Geodaetica et Geophysica     Hybrid Journal   (Followers: 3, SJR: 0.305, CiteScore: 1)
Acta Geophysica     Hybrid Journal   (Followers: 11, SJR: 0.312, CiteScore: 1)
Acta Geotechnica     Hybrid Journal   (Followers: 7, SJR: 1.588, CiteScore: 3)
Acta Informatica     Hybrid Journal   (Followers: 5, SJR: 0.517, CiteScore: 1)
Acta Mathematica     Hybrid Journal   (Followers: 12, SJR: 7.066, CiteScore: 3)
Acta Mathematica Hungarica     Hybrid Journal   (Followers: 2, SJR: 0.452, CiteScore: 1)
Acta Mathematica Sinica, English Series     Hybrid Journal   (Followers: 6, SJR: 0.379, CiteScore: 1)
Acta Mathematica Vietnamica     Hybrid Journal   (SJR: 0.27, CiteScore: 0)
Acta Mathematicae Applicatae Sinica, English Series     Hybrid Journal   (SJR: 0.208, CiteScore: 0)
Acta Mechanica     Hybrid Journal   (Followers: 25, SJR: 1.04, CiteScore: 2)
Acta Mechanica Sinica     Hybrid Journal   (Followers: 5, SJR: 0.607, CiteScore: 2)
Acta Metallurgica Sinica (English Letters)     Hybrid Journal   (Followers: 7, SJR: 0.576, CiteScore: 2)
Acta Meteorologica Sinica     Hybrid Journal   (Followers: 3, SJR: 0.638, CiteScore: 1)
Acta Neurochirurgica     Hybrid Journal   (Followers: 7, SJR: 0.822, CiteScore: 2)
Acta Neurologica Belgica     Hybrid Journal   (Followers: 2, SJR: 0.376, CiteScore: 1)
Acta Neuropathologica     Hybrid Journal   (Followers: 4, SJR: 7.589, CiteScore: 12)
Acta Oceanologica Sinica     Hybrid Journal   (Followers: 3, SJR: 0.334, CiteScore: 1)
Acta Physiologiae Plantarum     Hybrid Journal   (Followers: 4, SJR: 0.574, CiteScore: 2)
Acta Politica     Hybrid Journal   (Followers: 19, SJR: 0.605, CiteScore: 1)
Activitas Nervosa Superior     Hybrid Journal   (SJR: 0.147, CiteScore: 0)
Adaptive Human Behavior and Physiology     Hybrid Journal  
adhäsion KLEBEN & DICHTEN     Hybrid Journal   (Followers: 8, SJR: 0.103, CiteScore: 0)
ADHD Attention Deficit and Hyperactivity Disorders     Hybrid Journal   (Followers: 28, SJR: 0.72, CiteScore: 2)
Adhesion Adhesives & Sealants     Hybrid Journal   (Followers: 9)
Administration and Policy in Mental Health and Mental Health Services Research     Partially Free   (Followers: 19, SJR: 1.005, CiteScore: 2)
Adolescent Research Review     Hybrid Journal   (Followers: 1)
Adsorption     Hybrid Journal   (Followers: 5, SJR: 0.703, CiteScore: 2)
Advanced Composites and Hybrid Materials     Hybrid Journal  
Advanced Fiber Materials     Full-text available via subscription  
Advances in Applied Clifford Algebras     Hybrid Journal   (Followers: 4, SJR: 0.698, CiteScore: 1)
Advances in Astronautics Science and Technology     Hybrid Journal  
Advances in Atmospheric Sciences     Hybrid Journal   (Followers: 40, SJR: 0.956, CiteScore: 2)
Advances in Computational Mathematics     Hybrid Journal   (Followers: 21, SJR: 0.812, CiteScore: 1)
Advances in Contraception     Hybrid Journal   (Followers: 3)
Advances in Data Analysis and Classification     Hybrid Journal   (Followers: 58, SJR: 1.09, CiteScore: 1)
Advances in Gerontology     Partially Free   (Followers: 8, SJR: 0.144, CiteScore: 0)
Advances in Health Sciences Education     Hybrid Journal   (Followers: 35, SJR: 1.64, CiteScore: 2)
Advances in Manufacturing     Hybrid Journal   (Followers: 3, SJR: 0.475, CiteScore: 2)
Advances in Neurodevelopmental Disorders     Hybrid Journal  
Advances in Polymer Science     Hybrid Journal   (Followers: 49, SJR: 1.04, CiteScore: 3)
Advances in Therapy     Hybrid Journal   (Followers: 5, SJR: 1.075, CiteScore: 3)
Aegean Review of the Law of the Sea and Maritime Law     Hybrid Journal   (Followers: 7)
Aequationes Mathematicae     Hybrid Journal   (Followers: 2, SJR: 0.517, CiteScore: 1)
Aerobiologia     Hybrid Journal   (Followers: 3, SJR: 0.673, CiteScore: 2)
Aerosol Science and Engineering     Hybrid Journal  
Aerospace Systems     Hybrid Journal   (Followers: 1)
Aerotecnica Missili & Spazio : J. of Aerospace Science, Technologies & Systems     Hybrid Journal  
Aesthetic Plastic Surgery     Hybrid Journal   (Followers: 11, SJR: 0.825, CiteScore: 1)
African Archaeological Review     Hybrid Journal   (Followers: 21, SJR: 0.862, CiteScore: 1)
Afrika Matematika     Hybrid Journal   (Followers: 1, SJR: 0.235, CiteScore: 0)
Ageing Intl.     Hybrid Journal   (Followers: 7, SJR: 0.39, CiteScore: 1)
Aggiornamenti CIO     Hybrid Journal   (Followers: 1)
Aging Clinical and Experimental Research     Hybrid Journal   (Followers: 3, SJR: 0.67, CiteScore: 2)
Agricultural Research     Hybrid Journal   (Followers: 7, SJR: 0.276, CiteScore: 1)
Agriculture and Human Values     Open Access   (Followers: 15, SJR: 1.173, CiteScore: 3)
Agroforestry Systems     Open Access   (Followers: 20, SJR: 0.663, CiteScore: 1)
Agronomy for Sustainable Development     Open Access   (Followers: 15, SJR: 1.864, CiteScore: 6)
AI & Society     Hybrid Journal   (Followers: 9, SJR: 0.227, CiteScore: 1)
AIDS and Behavior     Hybrid Journal   (Followers: 16, SJR: 1.792, CiteScore: 3)
Air Quality, Atmosphere & Health     Hybrid Journal   (Followers: 4, SJR: 0.862, CiteScore: 3)
Akupunktur & Aurikulomedizin     Full-text available via subscription   (Followers: 1)
Algebra and Logic     Hybrid Journal   (Followers: 7, SJR: 0.531, CiteScore: 0)
Algebra Universalis     Hybrid Journal   (Followers: 2, SJR: 0.583, CiteScore: 1)
Algebras and Representation Theory     Hybrid Journal   (Followers: 1, SJR: 1.095, CiteScore: 1)
Algorithmica     Hybrid Journal   (Followers: 9, SJR: 0.56, CiteScore: 1)
Allergo J.     Full-text available via subscription   (Followers: 1, SJR: 0.234, CiteScore: 0)
Allergo J. Intl.     Hybrid Journal   (Followers: 2)
Alpine Botany     Hybrid Journal   (Followers: 5, SJR: 1.11, CiteScore: 3)
ALTEX : Alternatives to Animal Experimentation     Open Access   (Followers: 2)
AMBIO     Hybrid Journal   (Followers: 10, SJR: 1.569, CiteScore: 4)
American J. of Cardiovascular Drugs     Hybrid Journal   (Followers: 17, SJR: 0.951, CiteScore: 3)
American J. of Community Psychology     Hybrid Journal   (Followers: 29, SJR: 1.329, CiteScore: 2)
American J. of Criminal Justice     Hybrid Journal   (Followers: 9, SJR: 0.772, CiteScore: 1)
American J. of Cultural Sociology     Hybrid Journal   (Followers: 18, SJR: 0.46, CiteScore: 1)
American J. of Dance Therapy     Hybrid Journal   (Followers: 6, SJR: 0.181, CiteScore: 0)
American J. of Potato Research     Hybrid Journal   (Followers: 3, SJR: 0.611, CiteScore: 1)
American J. of Psychoanalysis     Hybrid Journal   (Followers: 22, SJR: 0.314, CiteScore: 0)
American Sociologist     Hybrid Journal   (Followers: 16, SJR: 0.35, CiteScore: 0)
Amino Acids     Hybrid Journal   (Followers: 7, SJR: 1.135, CiteScore: 3)
AMS Review     Partially Free   (Followers: 4)
Analog Integrated Circuits and Signal Processing     Hybrid Journal   (Followers: 10, SJR: 0.211, CiteScore: 1)
Analysis and Mathematical Physics     Hybrid Journal   (Followers: 6, SJR: 0.536, CiteScore: 1)
Analysis in Theory and Applications     Hybrid Journal   (Followers: 1)
Analysis of Verbal Behavior     Hybrid Journal   (Followers: 6)
Analytical and Bioanalytical Chemistry     Hybrid Journal   (Followers: 32, SJR: 0.978, CiteScore: 3)
Anatomical Science Intl.     Hybrid Journal   (Followers: 3, SJR: 0.367, CiteScore: 1)
Angewandte Schmerztherapie und Palliativmedizin     Hybrid Journal  
Angiogenesis     Hybrid Journal   (Followers: 3, SJR: 2.177, CiteScore: 5)
Animal Cognition     Hybrid Journal   (Followers: 23, SJR: 1.389, CiteScore: 3)
Annales françaises de médecine d'urgence     Hybrid Journal   (Followers: 1, SJR: 0.192, CiteScore: 0)
Annales Henri Poincaré     Hybrid Journal   (Followers: 3, SJR: 1.097, CiteScore: 2)
Annales mathématiques du Québec     Hybrid Journal   (Followers: 4, SJR: 0.438, CiteScore: 0)
Annali dell'Universita di Ferrara     Hybrid Journal   (SJR: 0.429, CiteScore: 0)
Annali di Matematica Pura ed Applicata     Hybrid Journal   (Followers: 1, SJR: 1.197, CiteScore: 1)
Annals of Biomedical Engineering     Hybrid Journal   (Followers: 19, SJR: 1.042, CiteScore: 3)
Annals of Combinatorics     Hybrid Journal   (Followers: 4, SJR: 0.932, CiteScore: 1)
Annals of Data Science     Hybrid Journal   (Followers: 13)
Annals of Dyslexia     Hybrid Journal   (Followers: 10, SJR: 0.85, CiteScore: 2)
Annals of Finance     Hybrid Journal   (Followers: 35, SJR: 0.579, CiteScore: 1)
Annals of Forest Science     Hybrid Journal   (Followers: 7, SJR: 0.986, CiteScore: 2)
Annals of Global Analysis and Geometry     Hybrid Journal   (Followers: 1, SJR: 1.228, CiteScore: 1)
Annals of Hematology     Hybrid Journal   (Followers: 15, SJR: 1.043, CiteScore: 2)
Annals of Mathematics and Artificial Intelligence     Hybrid Journal   (Followers: 13, SJR: 0.413, CiteScore: 1)
Annals of Microbiology     Hybrid Journal   (Followers: 13, SJR: 0.479, CiteScore: 2)
Annals of Nuclear Medicine     Hybrid Journal   (Followers: 5, SJR: 0.687, CiteScore: 2)
Annals of Operations Research     Hybrid Journal   (Followers: 11, SJR: 0.943, CiteScore: 2)
Annals of Ophthalmology     Hybrid Journal   (Followers: 13)
Annals of PDE     Hybrid Journal  
Annals of Regional Science     Hybrid Journal   (Followers: 9, SJR: 0.614, CiteScore: 1)
Annals of Software Engineering     Hybrid Journal   (Followers: 13)
Annals of Solid and Structural Mechanics     Hybrid Journal   (Followers: 11, SJR: 0.239, CiteScore: 1)
Annals of Surgical Oncology     Hybrid Journal   (Followers: 15, SJR: 1.986, CiteScore: 4)
Annals of Telecommunications     Hybrid Journal   (Followers: 9, SJR: 0.223, CiteScore: 1)
Annals of the Institute of Statistical Mathematics     Hybrid Journal   (Followers: 1, SJR: 1.495, CiteScore: 1)
Antonie van Leeuwenhoek     Hybrid Journal   (Followers: 5, SJR: 0.834, CiteScore: 2)
Apidologie     Hybrid Journal   (Followers: 4, SJR: 1.22, CiteScore: 3)
APOPTOSIS     Hybrid Journal   (Followers: 9, SJR: 1.424, CiteScore: 4)
Applicable Algebra in Engineering, Communication and Computing     Hybrid Journal   (Followers: 3, SJR: 0.294, CiteScore: 1)
Applications of Mathematics     Hybrid Journal   (Followers: 3, SJR: 0.602, CiteScore: 1)
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 44, SJR: 0.571, CiteScore: 2)
Applied Biochemistry and Microbiology     Hybrid Journal   (Followers: 19, SJR: 0.21, CiteScore: 1)
Applied Categorical Structures     Hybrid Journal   (Followers: 4, SJR: 0.49, CiteScore: 0)
Applied Composite Materials     Hybrid Journal   (Followers: 53, SJR: 0.58, CiteScore: 2)
Applied Entomology and Zoology     Partially Free   (Followers: 7, SJR: 0.422, CiteScore: 1)
Applied Geomatics     Hybrid Journal   (Followers: 3, SJR: 0.733, CiteScore: 3)
Applied Geophysics     Hybrid Journal   (Followers: 9, SJR: 0.488, CiteScore: 1)
Applied Intelligence     Hybrid Journal   (Followers: 15, SJR: 0.6, CiteScore: 2)
Applied Magnetic Resonance     Hybrid Journal   (Followers: 4, SJR: 0.319, CiteScore: 1)
Applied Mathematics & Optimization     Hybrid Journal   (Followers: 10, SJR: 0.886, CiteScore: 1)
Applied Mathematics - A J. of Chinese Universities     Hybrid Journal   (Followers: 1, SJR: 0.17, CiteScore: 0)
Applied Mathematics and Mechanics     Hybrid Journal   (Followers: 5, SJR: 0.461, CiteScore: 1)
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 68, SJR: 1.182, CiteScore: 4)
Applied Physics A     Hybrid Journal   (Followers: 10, SJR: 0.481, CiteScore: 2)
Applied Physics B: Lasers and Optics     Hybrid Journal   (Followers: 26, SJR: 0.74, CiteScore: 2)
Applied Psychophysiology and Biofeedback     Hybrid Journal   (Followers: 8, SJR: 0.519, CiteScore: 2)
Applied Research in Quality of Life     Hybrid Journal   (Followers: 12, SJR: 0.316, CiteScore: 1)
Applied Solar Energy     Hybrid Journal   (Followers: 22, SJR: 0.225, CiteScore: 0)
Applied Spatial Analysis and Policy     Hybrid Journal   (Followers: 6, SJR: 0.542, CiteScore: 1)
Aquaculture Intl.     Hybrid Journal   (Followers: 26, SJR: 0.591, CiteScore: 2)
Aquarium Sciences and Conservation     Hybrid Journal   (Followers: 2)
Aquatic Ecology     Hybrid Journal   (Followers: 37, SJR: 0.656, CiteScore: 2)
Aquatic Geochemistry     Hybrid Journal   (Followers: 3, SJR: 0.591, CiteScore: 1)
Aquatic Sciences     Hybrid Journal   (Followers: 14, SJR: 1.109, CiteScore: 3)
Arabian J. for Science and Engineering     Hybrid Journal   (Followers: 5, SJR: 0.303, CiteScore: 1)
Arabian J. of Geosciences     Hybrid Journal   (Followers: 2, SJR: 0.319, CiteScore: 1)
Archaeological and Anthropological Sciences     Hybrid Journal   (Followers: 22, SJR: 1.052, CiteScore: 2)
Archaeologies     Hybrid Journal   (Followers: 13, SJR: 0.224, CiteScore: 0)
Archiv der Mathematik     Hybrid Journal   (Followers: 1, SJR: 0.725, CiteScore: 1)
Archival Science     Hybrid Journal   (Followers: 68, SJR: 0.745, CiteScore: 2)
Archive for History of Exact Sciences     Hybrid Journal   (Followers: 7, SJR: 0.186, CiteScore: 1)
Archive for Mathematical Logic     Hybrid Journal   (Followers: 3, SJR: 0.909, CiteScore: 1)
Archive for Rational Mechanics and Analysis     Hybrid Journal   (SJR: 3.93, CiteScore: 3)
Archive of Applied Mechanics     Hybrid Journal   (Followers: 6, SJR: 0.79, CiteScore: 2)
Archives and Museum Informatics     Hybrid Journal   (Followers: 171, SJR: 0.101, CiteScore: 0)
Archives of Computational Methods in Engineering     Hybrid Journal   (Followers: 6, SJR: 1.41, CiteScore: 5)
Archives of Dermatological Research     Hybrid Journal   (Followers: 7, SJR: 1.006, CiteScore: 2)
Archives of Environmental Contamination and Toxicology     Hybrid Journal   (Followers: 14, SJR: 0.773, CiteScore: 2)
Archives of Gynecology and Obstetrics     Hybrid Journal   (Followers: 18, SJR: 0.956, CiteScore: 2)
Archives of Microbiology     Hybrid Journal   (Followers: 10, SJR: 0.644, CiteScore: 2)
Archives of Orthopaedic and Trauma Surgery     Hybrid Journal   (Followers: 9, SJR: 1.146, CiteScore: 2)
Archives of Osteoporosis     Hybrid Journal   (Followers: 2, SJR: 0.71, CiteScore: 2)
Archives of Sexual Behavior     Hybrid Journal   (Followers: 11, SJR: 1.493, CiteScore: 3)
Archives of Toxicology     Hybrid Journal   (Followers: 18, SJR: 1.541, CiteScore: 5)
Archives of Virology     Hybrid Journal   (Followers: 5, SJR: 0.973, CiteScore: 2)
Archives of Women's Mental Health     Hybrid Journal   (Followers: 17, SJR: 1.274, CiteScore: 3)
Archivio di Ortopedia e Reumatologia     Hybrid Journal  
Archivum Immunologiae et Therapiae Experimentalis     Hybrid Journal   (Followers: 2, SJR: 0.946, CiteScore: 3)
ArgoSpine News & J.     Hybrid Journal  
Argumentation     Hybrid Journal   (Followers: 6, SJR: 0.349, CiteScore: 1)
Arid Ecosystems     Hybrid Journal   (Followers: 3, SJR: 0.2, CiteScore: 0)
Arkiv för Matematik     Hybrid Journal   (Followers: 1, SJR: 0.766, CiteScore: 1)
arktos : The J. of Arctic Geosciences     Hybrid Journal  
Arnold Mathematical J.     Hybrid Journal   (Followers: 1, SJR: 0.355, CiteScore: 0)
Arthropod-Plant Interactions     Hybrid Journal   (Followers: 2, SJR: 0.839, CiteScore: 2)
Arthroskopie     Hybrid Journal   (Followers: 1, SJR: 0.131, CiteScore: 0)
Artificial Intelligence and Law     Hybrid Journal   (Followers: 12, SJR: 0.937, CiteScore: 2)
Artificial Intelligence Review     Hybrid Journal   (Followers: 22, SJR: 0.833, CiteScore: 4)
Artificial Life and Robotics     Hybrid Journal   (Followers: 10, SJR: 0.226, CiteScore: 0)
Asia Europe J.     Hybrid Journal   (Followers: 4, SJR: 0.504, CiteScore: 1)
Asia Pacific Education Review     Hybrid Journal   (Followers: 12, SJR: 0.479, CiteScore: 1)
Asia Pacific J. of Management     Hybrid Journal   (Followers: 17, SJR: 1.185, CiteScore: 2)

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Similar Journals
Journal Cover
Applied Biochemistry and Microbiology
Journal Prestige (SJR): 0.21
Citation Impact (citeScore): 1
Number of Followers: 19  
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1608-3024 - ISSN (Online) 0003-6838
Published by Springer-Verlag Homepage  [2573 journals]
  • Effect of the Method of Nanocomplex Synthesis on the Proteolytic Activity
           of Chymotrypsin Immobilized on Silver Nanoparticles
    • Abstract: Two methods for the synthesis of hybrid organic-inorganic nanocomplexes comprising chymotrypsin and silver nanoparticles have been developed based on the chemical reduction of silver nitrate by sodium borohydride. In the first method, the enzyme is added to the reaction mixture simultaneously with other reagents. In the second method, it is added to the reaction mixture at certain intervals after the start of the reduction reaction. Chymotrypsin immobilized on nanocomplexes stabilizes silver nanoparticles. As compared to the native enzyme, the immobilized enzyme prepared by the both methods exhibits an increased proteolytic activity in acidic and strongly alkaline media. At the same time, chymotrypsin immobilized on silver nanoparticles by the second method exhibits the highest level of proteolytic activity.
      PubDate: 2019-09-01
  • Effect of Salt Stress on Acetoin Metabolism of an Aroma-producing Strain
           Bacillus subtilis
    • Abstract: An aroma-producing strain B15 was isolated from a spontaneous fermentation culture of traditional Chinese sweet bean sauce (Tian Wei Food Co., Ltd., China), and identified as Bacillus subtilis, exhibiting an ability to synthesize acetoin. In order to better understand the influence of salt stress on acetoin metabolism, a metabolic network of B. subtilis for synthesis of acetoin was established, and the changes in levels of acetoin, glucose, lactic acid, amylase activity, cell dry weight and amino acids were measured during fermentation under 0.5, 5 and 10% NaCl. After the metabolic flux analysis of 3 important metabolite nodes (glucose-6-phosphate, fructose-6-phosphate and pyruvic acid) and the role of amino acids and NADPH in metabolic network, it was found that salt stress greatly affected the metabolic content of pyruvate, and the total carbon flux distribution of amino acids and NADPH decreased in different pathways along with the increase of salt concentration. The results provided a further in-depth investigation of B. subtilis strategies in the struggle for acetoin biosynthesis under salt stress.
      PubDate: 2019-09-01
  • Increased Productivity and Antifreeze Activity of Ice-binding Protein from
    • Abstract: Ice-binding proteins (IBPs) inhibit the growth and recrystallization of intracellular ice, enabling polar organisms to survive at subzero temperatures. IBPs are promising materials in biomedical applications such as cryopreservation and the hypothermic storage of cells, tissues, and organs. In this study, recombinant IBP from the antarctic bacterium Flavobacterium frigoris PS1 (FfIBP) was produced by Escherichia coli used as bioreactor, to examine the feasibility of scale-up. Oxygen transfer was the most important factor influencing cell growth and FfIBP production during pilot-scale fermentation. The final yield of recombinant FfIBP produced by E. coli harboring the pET28a-FfIBP vector system was 1.6 g/L, 3.8-fold higher than that from the previously published report using pCold I-FfIBP vector system, and its thermal hysteresis activity was 2.5°C at 9.7 µM. This study demonstrates the successful pilot-scale production of FfIBP.
      PubDate: 2019-09-01
  • Effect of Starch Composition on the Biosynthesis of Immunosuppressant
           Tacrolimus (FK-506) by Streptomyces tsukubaensis VKM Ac-2618D Strain
    • Abstract: The dependence of biosynthesis of macrolide immunosuppressive agent tacrolimus (FK-506) in starch-containing media by the Streptomyces tsukubaensis VKM Ac-2618D strain on the molecular weight and composition of potato starch polysaccharides was studied. It was shown that the use of high-molecular branched starches as a carbon source provided a higher tacrolimus yield as compared to linear low-molecular polysaccharides at the similar culture growth indicators. The approaches providing the highest rate of tacrolimus biosynthesis by using high- and low-molecular starches and dextrins have been proposed. The biosynthesis process carrying out under the periodic batch cultivation with fractional starch feeding increased the tacrolimus yield significantly (up to 2 times), maintained biomass at a high level and reduced destructive processes rates. The maximum tacrolimus titer was observed when high-molecular potato starches were added to the culture in the retardation growth phase, while the best positive effect for low-molecular starches and dextrins additives was noted at stationary growth phase. The results could be useful in creating biotechnology for the production of pharmaceutical tacrolimus.
      PubDate: 2019-09-01
  • Structural and Functional Characteristics of Hydrolytic Enzymes of
           Phytophagon Insects and Plant Protein Inhibitors (Review)
    • Abstract: The data on proteolytic, cellulolytic, proteolytic, and amylolytic enzymes of insect pests and their inhibitors from plants are considered and generalized. The structure, physical, chemical and functional properties of enzymes and inhibitors are described. The analyzed data showed that the search for possible ways to improve the inhibitor activity of insect hydrolases is relevant for the creation of pesticide alternatives.
      PubDate: 2019-09-01
  • Antioxidative System of Plants: Cellular Compartmentalization, Protective
           and Signaling Functions, Mechanisms of Regulation (Review)
    • Abstract: The current data on the generation and neutralization of reactive oxygen species in various compartments of plant cells are summarized. The role of antioxidants (AOs) in the protection of macromolecules and cellular structures from oxidative damage is considered. Special attention is paid to the participation of AOs in redox signaling and the regulation of cellular functions. The cross-link between the functioning of the AO system (AOS) and the nonspecific plant resistance to stressors is analyzed. The regulation of AOS in plants under the action of exogenous AOs, signal mediators, phytohormones, and other physiologically active substances, as well as by transgenesis, is considered.
      PubDate: 2019-09-01
  • Cloning, Heterologous Expression and Characterization of an Intracellular
           Serine Protease from Bacillus sp. LCB10
    • Abstract: An intracellular serine protease designated ISPr from newly isolated salt-tolerant Bacillus sp. LCB10 was expressed in Escherichia coli. The isp gene was cloned into pET30a vector and expressed in E. coli BL21 (DE3). After purification, the molecular mass of ISPr was estimated to be 35 kDa by SDS-PAGE and the specific activity was 384 U/mg. The protease showed optimal activity at 40°C and pH 10.0. ISPr was active and stable in wide range of alkaline pH. ISPr activity increased 1.8-fold in the presence of Mn2+ and was completely inhibited by PMSF. The enzyme exhibited high NaCl tolerance. The protease had a stable activity in the presence of 1–5% NaCl and retained 86% activity in the presence 7% NaCl, thus, this enzyme would have great potential in industry.
      PubDate: 2019-09-01
  • Pathogenicity and Lipid Composition of Mycelium of the Fungus Stagonospora
    • Abstract: The effect of the nitrogen source (sodium nitrate and soybean meal) on the growth, pathogenicity, and lipid composition of mycelium of Stagonospora cirsii Davis VIZR 1.41, a potential mycoherbicide against Canada thistle (Cirsium arvens (L.) Scop.), was studied. The fungus grew significantly (two times) faster on sucrose-soybean meal medium (SSM) than on modified Czapek medium (CM). The fungal mycelium during the exponential growth phase demonstrated the maximal pathogenicity level on the third day on SSM and on the sixth day on CM. Canada thistle leaf tissues were more susceptible (25% higher development of necrotic lesion) to mycelium obtained on SSM than to mycelium obtained on CM. The nitrogen source strongly affected the lipid composition of S. cirsii mycelium. In S. cirsii mycelium obtained on SSM, the total lipid content was 1.7 times lower; the ratio of nonpolar lipids to polar lipids was three times lower than that for fungal mycelium grown on CM. Considerable differences were found in the composition of nonpolar (sterols and fatty acid) and polar (sphingolipids) lipids with respect to the nitrogen source in the culture medium. The higher contents of sterols, free fatty acids, and some glycoceramides in the mycelium of S. cirsii obtained on SSM may be related to its higher pathogenicity level and can serve as a marker of mycoherbicide quality.
      PubDate: 2019-09-01
  • Activity of Neutral Phytase from Obesumbacterium proteus in Recombinant
           Strains of Yarrowia lipolytica under Cultivation on Low-Grade Vegetable
    • Abstract: The characteristics of recombinant phytase from Obesumbacterium proteus have been studied after its integration into Yarrowia lipolytica with a Po1f (pUV3-Op) plasmid. Phytase activity was observed in Y. lipolytica Po1f transformants in a wide pH range (3.5–7.5) and remained at 80% of the initial value after 3-min heating at 95°C, which made it possible to add the enzyme to a fodder produced with heat treatment. In the case of transformant cultivation on low-grade vegetable substrates, they also possessed confirmed phytase activity, as well as an increase in the content of intracellular phosphates as compared to cells of the wild-type Y. lipolytica strain.
      PubDate: 2019-09-01
  • Characteristics of the Chromatographic Cleaning and Protectiveness of the
           LcrV Isoform of Yersinia pestis
    • Abstract: Electrophoretic, homogeneous, highly purified preparations of various isoforms of the immunodominant antigen V (LcrV protein) of Yersinia pestis have been obtained via chromatographic purification. A significant similarity in the chromatographic profiles of the LcrV antigen isoforms of different strains with tryptophan (W113) and glutamic acid (E113) residues at position 113, which differed from the isoform profile containing glycine residue LcrV (G113), has been recorded. The latter is characterized by the highest antigenic and protective activity as compared with other protein isoforms. This isoform almost completely protected the immunized group of animals and had the greatest ability to induce the production of antibodies, the titers of which reached 1 : 250 000.
      PubDate: 2019-09-01
  • Campylobacter spp. and Typing Tools (Review)
    • Abstract: Campylobacter spp. are the principal bacteria causing human gastroenteritis globally. Recently, several species of Campylobacter other than C. jejuni and C. coli have been identified. These emerging species have been associated with undiagnosed intestinal infectious diseases, although their clinical importance has not been determined yet. In order to establish the implication of the emerging bacterial species in human gastroenteritis, several epidemiological studies have been performed, where molecular typing methods have played a central role. This review provides an overview of the most used molecular typing tools used to characterized Campylobacter species.
      PubDate: 2019-09-01
  • Isolation and Cleaning of Lactate Dehydrogenase from Pea ( Pisum sativum
           L.) Roots by Hypoxia and the Study of Its Regulatory Properties
    • Abstract: The placement of pea plants (Pisum sativum L.) under flooding conditions led to an increase in lactate dehydrogenase (EC activity in the roots. The enzyme was purified to an electrophoretic homogeneous state by a multistage purification method including ammonium sulfate fractionation, ion exchange chromatography on DEAE-Sephacel, and gel chromatography on Sephadex G-200. The degree of purification was 43.4, the yield was 2.5%, and the specific activity was 80.5 U/mg protein. Its physicochemical properties were studied: the molecular weight of the native lactate dehydrogenase molecule was 138 kDa. The molecular weight of the subunits was determined by PAGE by electrophoresis in the presence of DDS-Na. Its value was 34 kDa, which indicates that the enzyme is a homotetramer. The kinetic and regulatory properties of the enzyme and the values ​​of the Michaelis constants were established. he effect of the concentration of hydrogen ions and temperature on direct and reverse reactions catalyzed by it was obtained. It was determined that lactate dehydrogenase inhibited ATP.
      PubDate: 2019-09-01
  • Development of an Immunoenzyme Assay to Control the Total Content of
           Antibiotics of the Fluoroquinolone Group in Milk
    • Abstract: A method for preparing antibodies that specifically recognize antibiotics of the fluoroquinolone group with the same radical in the first position of the quinolone nucleus is proposed. The specificity of rabbit antisera prepared at different cycles of immunization by changing the structure of the hapten in the composition of immunogens was characterized. The selected antibodies provided a group-specific analysis of 16 representatives of fluoroquinolones, including a combination of the following compounds that are controlled in animal products: ciprofloxacin, norfloxacin, pefloxacin, ofloxacin, and enrofloxacin. Using these antibodies, an indirect competitive enzyme-linked immunosorbent assay was developed that was characterized by a detection limit of ciprofloxacin of 0.2 ng/mL and a duration of 2 h. The assay was approbated for the detection of fluoroquinolones in milk.
      PubDate: 2019-09-01
  • Synthesis and Characterization of Polyaspartic Acid-Histidine Conjugate as
           an Analog of Antioxidant Enzymes
    • Abstract: Polyamino acids are versatile macromolecules with potential to be used as bioactive compounds and drug carriers. Polyaspartic acid (pAsp) and amide conjugate of pAsp with L-histidine (pAsp–His) were synthesized and their catalytic antioxidant properties were studied. pAsp–His did not exhibit the ability to decompose hydrogen peroxide. According to the redox reaction of generation of superoxide radical (SOR) by NADH/phenazine methosulfate, pAsp–His at a concentration of ≥0.1 mg/mL showed superoxide dismutase (SOD)-like activity similar to that of SOD-2 (100 U/mL). In addition, we showed that L-histidine in composition with manganese (II) and copper (II) ions catalyzed SOR dismutation in proportion to the amino acid concentration. SOD-like activity of pAsp–His was observed without addition of metal cofactors. The conjugate caused some cytotoxic effect towards cancer cells (PC-3 line, IC50 ≈ 0.8 mg/mL, 72 h) and modulated viability human skin fibroblasts. The results are of particular interest for the development of bioactive peptides, which mimic action of natural enzymes.
      PubDate: 2019-09-01
  • Detection of RNA Hydrolysis with Binase by Acridine Orange Fluorescence
    • Abstract: Fluorescence spectroscopy was used to study the specific hydrolysis of ribosomal RNA by binase in aqueous buffer solutions. Acridine orange was used for RNA staining. When the dye was bound to RNA, its fluorescence intensity increased by two times due to the formation of a stable complex. During RNA hydrolysis by binase for 1–20 min, this complex was cleaved, which was accompanied by a nearly twofold decrease in fluorescence. The degree of polarization of the dye fluorescence during hydrolysis was reduced by a factor of 5.5. The hydrolysis reaction was slower at a pH of 5.0–6.0 than at a pH of 7.0–8.0, but it proceeded almost until the end. Hydrolysis was slowed with an increase in the ionic strength of the buffer and was suppressed by magnesium ions. The studied reaction can be further used as a convenient, selective, fluorometric method for the detection of single-stranded ribosomal RNA and the study of their properties.
      PubDate: 2019-09-01
  • Membranolytic Effects of KT2 on Gram-Negative Escherichia coli Evaluated
           by Atomic Force Microscopy
    • Abstract: KT2 is a cationic antimicrobial peptide belonging to Crocodylus siamensis leucrocin I analogs. The mode of action of this compound taken at lethal concentration includes translocation into bacterial cells where binding to DNA is presumed to occur. However, the effects of KT2 on bacterial membrane have not been completely elucidated to date. In this study, a LIVE/DEAD staining technique was used to estimate the appropriate time of peptide-bacteria interaction. The results indicated more than 90% of Escherichia coli population was killed at density of ∼5 × 108 CFU/mL within 30 min after treatment with KT2 at MIC and 10 × MIC. The effects of KT2 on bacterial cells were investigated by the atomic force microscopy (AFM). At near MICs, the peptide induced heavy indentation of the bacterial surface as well as cellular collapse. Conversely, at concentrations of several times the MIC the potential to kill bacteria was greatly increased as judged by the induction of multiple membrane buds on the cell surface. Therefore, the collected results indicate that KT2 can cause different effects on bacterial surface which are positively correlated in magnitude and severity with peptide concentration via membranolytic effects.
      PubDate: 2019-09-01
  • Sodium Alginate Based Mucoadhesive Hydrogels
    • Abstract: The potential use of sodium-alginate hydrogels as mucoadhesive drug delivery carriers for intranasal application is studied. It is demonstrated that the drug release rate is dependent on the drug solubility in water, the drug concentration in the carrier, and the degree of alginate crosslinking.
      PubDate: 2019-07-01
  • Influence of the Main Characteristics of Low Weight Chitosan on the Growth
           of the Plant Pathogenic Fungus Botrytis сinerea
    • Abstract: The effect of the main characteristics of low molecular weight chitosan obtained via enzymatic and chemical hydrolysis with a molecular mass (MM) of 2–100 kDa, a deacetylation degree (DD) of 60–98%, and a polydispersity (Mw/Mn) of 1.9–2.7 on the growth of Botrytis cinerea has been studied. The greatest inhibitory effect on the fungal germination process (a conidia germination index of less than 50%) was provided by chitosan samples with an MM of 2–13 kDa, a DD of 85–98%, and a polydispersity of 2–2.5. Among the most effective was chitosan with an MM of 13 kDa and a deacetylation degree 98%, which had a significant inhibitory effect on the growth of the fungal mycelium at concentration 0.938 mg/mL. This sample of chitosan and its complex with copper ions (75 ppm) significantly suppressed fungal metabolic activity (up to 20 and 10%, respectively), which indicates their high potential as antifungal agents for the development of fungicides.
      PubDate: 2019-07-01
  • Biotechnological Aspects of the Enzymatic Preparation of Bioactive
           Chitooligosaccharides (Review)
    • Abstract: The review describes recent technologies for the enzymatic preparation of chitooligosaccharides with chitosanases. Features of the microbial synthesis of chitosanases and approaches to the implementation of their biotechnological production are also highlighted. Tendencies in the development of methods and technologies for the targeted preparation of chitosan oligomers with the use of bioreactors and immobilized enzymes are considered.
      PubDate: 2019-07-01
  • Characterization and Construction of Acinetobacter calcoaceticus T32
           Strain that Can Remove Ammonia Nitrogen and Mercury
    • Abstract: Acinetobacter calcoaceticus has been reported to be a good candidate for removing various pollutants. In this study, A. calcoaceticus T32 could remove 100% \({\text{NH}}_{4}^{ + }\) -N and 98% of total inorganic nitrogen within 48 h, with production of traces of \({\text{NO}}_{2}^{ - }\) and \({\text{NO}}_{3}^{ - }\) . The gene of subunit of ammonia monooxygenase (AMO), amoAAc, was cloned from A. calcoaceticus. Real-time reverse transcriptase PCR showed that mRNA level of amoAAc was 22-fold higher when \({\text{NH}}_{4}^{ + }\) was present, and retained to normal level in the presence of 50 mg/L allylthiourea, an inhibitor of AMO. mer operon containing merT, merP and merA genes involved in mercury resistance was fused into plasmid JR20 and introduced into A. calcoaceticus T32 through bacterial conjugation, constructing A. calcoaceticus T32/JRHg, which removed 93% HgCl2. LD50 of A. calcoaceticus T32 was 6.19 × 108 CFU using model with Japanese flounder, suggesting that A. calcoaceticus T32 might be environmentally friendly to treat nitrogen and mercury pollutants.
      PubDate: 2019-07-01
School of Mathematical and Computer Sciences
Heriot-Watt University
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