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Publisher: Springer-Verlag (Total: 2351 journals)

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        1 2 3 4 5 6 7 8 | Last   [Sort by number of followers]   [Restore default list]

Showing 1 - 200 of 2351 Journals sorted alphabetically
3D Printing in Medicine     Open Access  
3D Research     Hybrid Journal   (Followers: 21, SJR: 0.222, CiteScore: 1)
4OR: A Quarterly J. of Operations Research     Hybrid Journal   (Followers: 10, SJR: 0.825, CiteScore: 1)
AAPS J.     Hybrid Journal   (Followers: 22, SJR: 1.118, CiteScore: 4)
AAPS PharmSciTech     Hybrid Journal   (Followers: 7, SJR: 0.752, CiteScore: 3)
Abdominal Imaging     Hybrid Journal   (Followers: 14, SJR: 0.866, CiteScore: 2)
Abhandlungen aus dem Mathematischen Seminar der Universitat Hamburg     Hybrid Journal   (Followers: 4, SJR: 0.439, CiteScore: 0)
Academic Psychiatry     Full-text available via subscription   (Followers: 23, SJR: 0.53, CiteScore: 1)
Academic Questions     Hybrid Journal   (Followers: 8, SJR: 0.106, CiteScore: 0)
Accreditation and Quality Assurance: J. for Quality, Comparability and Reliability in Chemical Measurement     Hybrid Journal   (Followers: 26, SJR: 0.316, CiteScore: 1)
Acoustical Physics     Hybrid Journal   (Followers: 11, SJR: 0.359, CiteScore: 1)
Acoustics Australia     Hybrid Journal   (SJR: 0.232, CiteScore: 1)
Acta Analytica     Hybrid Journal   (Followers: 7, SJR: 0.367, CiteScore: 0)
Acta Applicandae Mathematicae     Hybrid Journal   (Followers: 1, SJR: 0.675, CiteScore: 1)
Acta Biotheoretica     Hybrid Journal   (Followers: 4, SJR: 0.284, CiteScore: 1)
Acta Diabetologica     Hybrid Journal   (Followers: 18, SJR: 1.587, CiteScore: 3)
Acta Endoscopica     Hybrid Journal   (Followers: 1)
acta ethologica     Hybrid Journal   (Followers: 4, SJR: 0.769, CiteScore: 1)
Acta Geochimica     Hybrid Journal   (Followers: 6, SJR: 0.24, CiteScore: 1)
Acta Geodaetica et Geophysica     Hybrid Journal   (Followers: 2, SJR: 0.305, CiteScore: 1)
Acta Geotechnica     Hybrid Journal   (Followers: 7, SJR: 1.588, CiteScore: 3)
Acta Informatica     Hybrid Journal   (Followers: 5, SJR: 0.517, CiteScore: 1)
Acta Mathematica     Hybrid Journal   (Followers: 12, SJR: 7.066, CiteScore: 3)
Acta Mathematica Hungarica     Hybrid Journal   (Followers: 2, SJR: 0.452, CiteScore: 1)
Acta Mathematica Sinica, English Series     Hybrid Journal   (Followers: 6, SJR: 0.379, CiteScore: 1)
Acta Mathematica Vietnamica     Hybrid Journal   (SJR: 0.27, CiteScore: 0)
Acta Mathematicae Applicatae Sinica, English Series     Hybrid Journal   (SJR: 0.208, CiteScore: 0)
Acta Mechanica     Hybrid Journal   (Followers: 21, SJR: 1.04, CiteScore: 2)
Acta Mechanica Sinica     Hybrid Journal   (Followers: 5, SJR: 0.607, CiteScore: 2)
Acta Metallurgica Sinica (English Letters)     Hybrid Journal   (Followers: 7, SJR: 0.576, CiteScore: 2)
Acta Meteorologica Sinica     Hybrid Journal   (Followers: 3, SJR: 0.638, CiteScore: 1)
Acta Neurochirurgica     Hybrid Journal   (Followers: 6, SJR: 0.822, CiteScore: 2)
Acta Neurologica Belgica     Hybrid Journal   (Followers: 1, SJR: 0.376, CiteScore: 1)
Acta Neuropathologica     Hybrid Journal   (Followers: 5, SJR: 7.589, CiteScore: 12)
Acta Oceanologica Sinica     Hybrid Journal   (Followers: 3, SJR: 0.334, CiteScore: 1)
Acta Parasitologica     Hybrid Journal   (Followers: 10, SJR: 0.641, CiteScore: 1)
Acta Physiologiae Plantarum     Hybrid Journal   (Followers: 2, SJR: 0.574, CiteScore: 2)
Acta Politica     Hybrid Journal   (Followers: 14, SJR: 0.605, CiteScore: 1)
Activitas Nervosa Superior     Hybrid Journal   (SJR: 0.147, CiteScore: 0)
adhäsion KLEBEN & DICHTEN     Hybrid Journal   (Followers: 7, SJR: 0.103, CiteScore: 0)
ADHD Attention Deficit and Hyperactivity Disorders     Hybrid Journal   (Followers: 23, SJR: 0.72, CiteScore: 2)
Adhesion Adhesives & Sealants     Hybrid Journal   (Followers: 9)
Administration and Policy in Mental Health and Mental Health Services Research     Partially Free   (Followers: 16, SJR: 1.005, CiteScore: 2)
Adsorption     Hybrid Journal   (Followers: 4, SJR: 0.703, CiteScore: 2)
Advances in Applied Clifford Algebras     Hybrid Journal   (Followers: 4, SJR: 0.698, CiteScore: 1)
Advances in Atmospheric Sciences     Hybrid Journal   (Followers: 37, SJR: 0.956, CiteScore: 2)
Advances in Computational Mathematics     Hybrid Journal   (Followers: 19, SJR: 0.812, CiteScore: 1)
Advances in Contraception     Hybrid Journal   (Followers: 3)
Advances in Data Analysis and Classification     Hybrid Journal   (Followers: 51, SJR: 1.09, CiteScore: 1)
Advances in Gerontology     Partially Free   (Followers: 8, SJR: 0.144, CiteScore: 0)
Advances in Health Sciences Education     Hybrid Journal   (Followers: 29, SJR: 1.64, CiteScore: 2)
Advances in Manufacturing     Hybrid Journal   (Followers: 3, SJR: 0.475, CiteScore: 2)
Advances in Polymer Science     Hybrid Journal   (Followers: 43, SJR: 1.04, CiteScore: 3)
Advances in Therapy     Hybrid Journal   (Followers: 5, SJR: 1.075, CiteScore: 3)
Aegean Review of the Law of the Sea and Maritime Law     Hybrid Journal   (Followers: 6)
Aequationes Mathematicae     Hybrid Journal   (Followers: 2, SJR: 0.517, CiteScore: 1)
Aerobiologia     Hybrid Journal   (Followers: 3, SJR: 0.673, CiteScore: 2)
Aesthetic Plastic Surgery     Hybrid Journal   (Followers: 9, SJR: 0.825, CiteScore: 1)
African Archaeological Review     Hybrid Journal   (Followers: 16, SJR: 0.862, CiteScore: 1)
Afrika Matematika     Hybrid Journal   (Followers: 1, SJR: 0.235, CiteScore: 0)
AGE     Hybrid Journal   (Followers: 7)
Ageing Intl.     Hybrid Journal   (Followers: 7, SJR: 0.39, CiteScore: 1)
Aggiornamenti CIO     Hybrid Journal   (Followers: 1)
Aging Clinical and Experimental Research     Hybrid Journal   (Followers: 3, SJR: 0.67, CiteScore: 2)
Agricultural Research     Hybrid Journal   (Followers: 5, SJR: 0.276, CiteScore: 1)
Agriculture and Human Values     Hybrid Journal   (Followers: 14, SJR: 1.173, CiteScore: 3)
Agroforestry Systems     Hybrid Journal   (Followers: 19, SJR: 0.663, CiteScore: 1)
Agronomy for Sustainable Development     Hybrid Journal   (Followers: 12, SJR: 1.864, CiteScore: 6)
AI & Society     Hybrid Journal   (Followers: 8, SJR: 0.227, CiteScore: 1)
AIDS and Behavior     Hybrid Journal   (Followers: 14, SJR: 1.792, CiteScore: 3)
Air Quality, Atmosphere & Health     Hybrid Journal   (Followers: 4, SJR: 0.862, CiteScore: 3)
Akupunktur & Aurikulomedizin     Full-text available via subscription   (Followers: 1)
Algebra and Logic     Hybrid Journal   (Followers: 6, SJR: 0.531, CiteScore: 0)
Algebra Universalis     Hybrid Journal   (Followers: 2, SJR: 0.583, CiteScore: 1)
Algebras and Representation Theory     Hybrid Journal   (Followers: 1, SJR: 1.095, CiteScore: 1)
Algorithmica     Hybrid Journal   (Followers: 9, SJR: 0.56, CiteScore: 1)
Allergo J.     Full-text available via subscription   (Followers: 1, SJR: 0.234, CiteScore: 0)
Allergo J. Intl.     Hybrid Journal   (Followers: 2)
Alpine Botany     Hybrid Journal   (Followers: 5, SJR: 1.11, CiteScore: 3)
ALTEX : Alternatives to Animal Experimentation     Open Access   (Followers: 3)
AMBIO     Hybrid Journal   (Followers: 10, SJR: 1.569, CiteScore: 4)
American J. of Cardiovascular Drugs     Hybrid Journal   (Followers: 16, SJR: 0.951, CiteScore: 3)
American J. of Community Psychology     Hybrid Journal   (Followers: 28, SJR: 1.329, CiteScore: 2)
American J. of Criminal Justice     Hybrid Journal   (Followers: 8, SJR: 0.772, CiteScore: 1)
American J. of Cultural Sociology     Hybrid Journal   (Followers: 16, SJR: 0.46, CiteScore: 1)
American J. of Dance Therapy     Hybrid Journal   (Followers: 4, SJR: 0.181, CiteScore: 0)
American J. of Potato Research     Hybrid Journal   (Followers: 2, SJR: 0.611, CiteScore: 1)
American J. of Psychoanalysis     Hybrid Journal   (Followers: 21, SJR: 0.314, CiteScore: 0)
American Sociologist     Hybrid Journal   (Followers: 12, SJR: 0.35, CiteScore: 0)
Amino Acids     Hybrid Journal   (Followers: 8, SJR: 1.135, CiteScore: 3)
AMS Review     Partially Free   (Followers: 4)
Analog Integrated Circuits and Signal Processing     Hybrid Journal   (Followers: 7, SJR: 0.211, CiteScore: 1)
Analysis and Mathematical Physics     Hybrid Journal   (Followers: 5, SJR: 0.536, CiteScore: 1)
Analysis in Theory and Applications     Hybrid Journal   (Followers: 1)
Analysis of Verbal Behavior     Hybrid Journal   (Followers: 5)
Analytical and Bioanalytical Chemistry     Hybrid Journal   (Followers: 32, SJR: 0.978, CiteScore: 3)
Anatomical Science Intl.     Hybrid Journal   (Followers: 2, SJR: 0.367, CiteScore: 1)
Angewandte Schmerztherapie und Palliativmedizin     Hybrid Journal  
Angiogenesis     Hybrid Journal   (Followers: 3, SJR: 2.177, CiteScore: 5)
Animal Cognition     Hybrid Journal   (Followers: 19, SJR: 1.389, CiteScore: 3)
Annales françaises de médecine d'urgence     Hybrid Journal   (Followers: 1, SJR: 0.192, CiteScore: 0)
Annales Henri Poincaré     Hybrid Journal   (Followers: 3, SJR: 1.097, CiteScore: 2)
Annales mathématiques du Québec     Hybrid Journal   (Followers: 4, SJR: 0.438, CiteScore: 0)
Annali dell'Universita di Ferrara     Hybrid Journal   (SJR: 0.429, CiteScore: 0)
Annali di Matematica Pura ed Applicata     Hybrid Journal   (Followers: 1, SJR: 1.197, CiteScore: 1)
Annals of Biomedical Engineering     Hybrid Journal   (Followers: 18, SJR: 1.042, CiteScore: 3)
Annals of Combinatorics     Hybrid Journal   (Followers: 4, SJR: 0.932, CiteScore: 1)
Annals of Data Science     Hybrid Journal   (Followers: 11)
Annals of Dyslexia     Hybrid Journal   (Followers: 10, SJR: 0.85, CiteScore: 2)
Annals of Finance     Hybrid Journal   (Followers: 30, SJR: 0.579, CiteScore: 1)
Annals of Forest Science     Hybrid Journal   (Followers: 7, SJR: 0.986, CiteScore: 2)
Annals of Global Analysis and Geometry     Hybrid Journal   (Followers: 1, SJR: 1.228, CiteScore: 1)
Annals of Hematology     Hybrid Journal   (Followers: 15, SJR: 1.043, CiteScore: 2)
Annals of Mathematics and Artificial Intelligence     Hybrid Journal   (Followers: 12, SJR: 0.413, CiteScore: 1)
Annals of Microbiology     Hybrid Journal   (Followers: 10, SJR: 0.479, CiteScore: 2)
Annals of Nuclear Medicine     Hybrid Journal   (Followers: 4, SJR: 0.687, CiteScore: 2)
Annals of Operations Research     Hybrid Journal   (Followers: 10, SJR: 0.943, CiteScore: 2)
Annals of Ophthalmology     Hybrid Journal   (Followers: 11)
Annals of Regional Science     Hybrid Journal   (Followers: 7, SJR: 0.614, CiteScore: 1)
Annals of Software Engineering     Hybrid Journal   (Followers: 13)
Annals of Solid and Structural Mechanics     Hybrid Journal   (Followers: 9, SJR: 0.239, CiteScore: 1)
Annals of Surgical Oncology     Hybrid Journal   (Followers: 13, SJR: 1.986, CiteScore: 4)
Annals of Telecommunications     Hybrid Journal   (Followers: 9, SJR: 0.223, CiteScore: 1)
Annals of the Institute of Statistical Mathematics     Hybrid Journal   (Followers: 1, SJR: 1.495, CiteScore: 1)
Antonie van Leeuwenhoek     Hybrid Journal   (Followers: 5, SJR: 0.834, CiteScore: 2)
Apidologie     Hybrid Journal   (Followers: 4, SJR: 1.22, CiteScore: 3)
APOPTOSIS     Hybrid Journal   (Followers: 8, SJR: 1.424, CiteScore: 4)
Applicable Algebra in Engineering, Communication and Computing     Hybrid Journal   (Followers: 2, SJR: 0.294, CiteScore: 1)
Applications of Mathematics     Hybrid Journal   (Followers: 2, SJR: 0.602, CiteScore: 1)
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 43, SJR: 0.571, CiteScore: 2)
Applied Biochemistry and Microbiology     Hybrid Journal   (Followers: 17, SJR: 0.21, CiteScore: 1)
Applied Cancer Research     Open Access  
Applied Categorical Structures     Hybrid Journal   (Followers: 2, SJR: 0.49, CiteScore: 0)
Applied Composite Materials     Hybrid Journal   (Followers: 49, SJR: 0.58, CiteScore: 2)
Applied Entomology and Zoology     Partially Free   (Followers: 3, SJR: 0.422, CiteScore: 1)
Applied Geomatics     Hybrid Journal   (Followers: 3, SJR: 0.733, CiteScore: 3)
Applied Geophysics     Hybrid Journal   (Followers: 8, SJR: 0.488, CiteScore: 1)
Applied Intelligence     Hybrid Journal   (Followers: 12, SJR: 0.6, CiteScore: 2)
Applied Magnetic Resonance     Hybrid Journal   (Followers: 4, SJR: 0.319, CiteScore: 1)
Applied Mathematics & Optimization     Hybrid Journal   (Followers: 6, SJR: 0.886, CiteScore: 1)
Applied Mathematics - A J. of Chinese Universities     Hybrid Journal   (SJR: 0.17, CiteScore: 0)
Applied Mathematics and Mechanics     Hybrid Journal   (Followers: 5, SJR: 0.461, CiteScore: 1)
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 63, SJR: 1.182, CiteScore: 4)
Applied Physics A     Hybrid Journal   (Followers: 9, SJR: 0.481, CiteScore: 2)
Applied Physics B: Lasers and Optics     Hybrid Journal   (Followers: 24, SJR: 0.74, CiteScore: 2)
Applied Psychophysiology and Biofeedback     Hybrid Journal   (Followers: 8, SJR: 0.519, CiteScore: 2)
Applied Research in Quality of Life     Hybrid Journal   (Followers: 12, SJR: 0.316, CiteScore: 1)
Applied Solar Energy     Hybrid Journal   (Followers: 18, SJR: 0.225, CiteScore: 0)
Applied Spatial Analysis and Policy     Hybrid Journal   (Followers: 4, SJR: 0.542, CiteScore: 1)
Aquaculture Intl.     Hybrid Journal   (Followers: 23, SJR: 0.591, CiteScore: 2)
Aquarium Sciences and Conservation     Hybrid Journal   (Followers: 1)
Aquatic Ecology     Hybrid Journal   (Followers: 33, SJR: 0.656, CiteScore: 2)
Aquatic Geochemistry     Hybrid Journal   (Followers: 4, SJR: 0.591, CiteScore: 1)
Aquatic Sciences     Hybrid Journal   (Followers: 13, SJR: 1.109, CiteScore: 3)
Arabian J. for Science and Engineering     Hybrid Journal   (Followers: 5, SJR: 0.303, CiteScore: 1)
Arabian J. of Geosciences     Hybrid Journal   (Followers: 2, SJR: 0.319, CiteScore: 1)
Archaeological and Anthropological Sciences     Hybrid Journal   (Followers: 20, SJR: 1.052, CiteScore: 2)
Archaeologies     Hybrid Journal   (Followers: 12, SJR: 0.224, CiteScore: 0)
Archiv der Mathematik     Hybrid Journal   (Followers: 1, SJR: 0.725, CiteScore: 1)
Archival Science     Hybrid Journal   (Followers: 60, SJR: 0.745, CiteScore: 2)
Archive for History of Exact Sciences     Hybrid Journal   (Followers: 8, SJR: 0.186, CiteScore: 1)
Archive for Mathematical Logic     Hybrid Journal   (Followers: 2, SJR: 0.909, CiteScore: 1)
Archive for Rational Mechanics and Analysis     Hybrid Journal   (SJR: 3.93, CiteScore: 3)
Archive of Applied Mechanics     Hybrid Journal   (Followers: 5, SJR: 0.79, CiteScore: 2)
Archives and Museum Informatics     Hybrid Journal   (Followers: 144, SJR: 0.101, CiteScore: 0)
Archives of Computational Methods in Engineering     Hybrid Journal   (Followers: 5, SJR: 1.41, CiteScore: 5)
Archives of Dermatological Research     Hybrid Journal   (Followers: 7, SJR: 1.006, CiteScore: 2)
Archives of Environmental Contamination and Toxicology     Hybrid Journal   (Followers: 14, SJR: 0.773, CiteScore: 2)
Archives of Gynecology and Obstetrics     Hybrid Journal   (Followers: 16, SJR: 0.956, CiteScore: 2)
Archives of Microbiology     Hybrid Journal   (Followers: 8, SJR: 0.644, CiteScore: 2)
Archives of Orthopaedic and Trauma Surgery     Hybrid Journal   (Followers: 8, SJR: 1.146, CiteScore: 2)
Archives of Osteoporosis     Hybrid Journal   (Followers: 2, SJR: 0.71, CiteScore: 2)
Archives of Sexual Behavior     Hybrid Journal   (Followers: 10, SJR: 1.493, CiteScore: 3)
Archives of Toxicology     Hybrid Journal   (Followers: 17, SJR: 1.541, CiteScore: 5)
Archives of Virology     Hybrid Journal   (Followers: 5, SJR: 0.973, CiteScore: 2)
Archives of Women's Mental Health     Hybrid Journal   (Followers: 14, SJR: 1.274, CiteScore: 3)
Archivio di Ortopedia e Reumatologia     Hybrid Journal  
Archivum Immunologiae et Therapiae Experimentalis     Hybrid Journal   (Followers: 2, SJR: 0.946, CiteScore: 3)
ArgoSpine News & J.     Hybrid Journal  
Argumentation     Hybrid Journal   (Followers: 5, SJR: 0.349, CiteScore: 1)
Arid Ecosystems     Hybrid Journal   (Followers: 2, SJR: 0.2, CiteScore: 0)
Arkiv för Matematik     Hybrid Journal   (Followers: 1, SJR: 0.766, CiteScore: 1)
Arnold Mathematical J.     Hybrid Journal   (Followers: 1, SJR: 0.355, CiteScore: 0)
Arthropod-Plant Interactions     Hybrid Journal   (Followers: 2, SJR: 0.839, CiteScore: 2)
Arthroskopie     Hybrid Journal   (Followers: 1, SJR: 0.131, CiteScore: 0)
Artificial Intelligence and Law     Hybrid Journal   (Followers: 11, SJR: 0.937, CiteScore: 2)
Artificial Intelligence Review     Hybrid Journal   (Followers: 14, SJR: 0.833, CiteScore: 4)
Artificial Life and Robotics     Hybrid Journal   (Followers: 9, SJR: 0.226, CiteScore: 0)
Asia Europe J.     Hybrid Journal   (Followers: 5, SJR: 0.504, CiteScore: 1)
Asia Pacific Education Review     Hybrid Journal   (Followers: 12, SJR: 0.479, CiteScore: 1)
Asia Pacific J. of Management     Hybrid Journal   (Followers: 16, SJR: 1.185, CiteScore: 2)
Asia-Pacific Education Researcher     Hybrid Journal   (Followers: 12, SJR: 0.353, CiteScore: 1)
Asia-Pacific Financial Markets     Hybrid Journal   (Followers: 2, SJR: 0.187, CiteScore: 0)
Asia-Pacific J. of Atmospheric Sciences     Hybrid Journal   (Followers: 19, SJR: 0.855, CiteScore: 1)
Asian Business & Management     Hybrid Journal   (Followers: 9, SJR: 0.378, CiteScore: 1)
Asian J. of Business Ethics     Hybrid Journal   (Followers: 9)
Asian J. of Criminology     Hybrid Journal   (Followers: 5, SJR: 0.543, CiteScore: 1)
AStA Advances in Statistical Analysis     Hybrid Journal   (Followers: 3, SJR: 0.548, CiteScore: 1)
AStA Wirtschafts- und Sozialstatistisches Archiv     Hybrid Journal   (Followers: 5, SJR: 0.183, CiteScore: 0)
ästhetische dermatologie & kosmetologie     Full-text available via subscription  

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Journal Cover
Applied Biochemistry and Microbiology
Journal Prestige (SJR): 0.21
Citation Impact (citeScore): 1
Number of Followers: 17  
 
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1608-3024 - ISSN (Online) 0003-6838
Published by Springer-Verlag Homepage  [2351 journals]
  • Natural Polyphenols: Biological Activity, Pharmacological Potential, Means
           of Metabolic Engineering (Review)
    • Authors: V. V. Teplova; E. P. Isakova; O. I. Klein; D. I. Dergachova; N. N. Gessler; Y. I. Deryabina
      Pages: 221 - 237
      Abstract: This review examines the main features of natural phytoalexines of flavonoid and stilbenoid natures, which are secondary metabolism products in numerous plants widely used as biologically active substances in the medicine, pharmacology, and agricultural plants protection. We considered the role of flavonoids and stilbenes in phytoimmune and antistress responses in plants, bactericide antifungul, and antiviral effects towards microorganisms, and the wide medical application for a number of mammalian pathologies. The main achievements in the metabolic engineering of flavonoids in microbial biotechnologies are discussed.
      PubDate: 2018-05-01
      DOI: 10.1134/s0003683818030146
      Issue No: Vol. 54, No. 3 (2018)
       
  • Construction of a Synthetic Bypass for Improvement of Aerobic Synthesis of
           Succinic Acid through the Oxidative Branch of the Tricarboxylic Acid Cycle
           by Recombinant Escherichia coli Strains
    • Authors: A. Yu. Skorokhodova; A. A. Stasenko; A. Yu. Gulevich; V. G. Debabov
      Pages: 245 - 251
      Abstract: The effect of the introduction of a synthetic bypass, providing 2-ketoglutarate to succinate conversion via the intermediate succinate semialdehyde formation, on aerobic biosynthesis of succinic acid from glucose through the oxidative branch of the tricarboxylic acid cycle in recombinant Escherichia coli strains has been studied. The strain lacking the key pathways of acetic, lactic acid and ethanol formation from pyruvate and acetyl-CoA and possessing modified system of glucose transport and phosphorylation was used as a chassis for the construction of the target recombinants. The operation of the glyoxylate shunt in the strains was precluded resulting from the deletion of the aceA, aceB, and glcB genes encoding isocitrate lyase and malate synthases A and G. The constitutive activity of isocitrate dehydrogenase was ensured due to deletion of isocitrate dehydrogenase kinase/phosphatase gene, aceK. Upon further inactivation of succinate dehydrogenase, the corresponding strain synthesized succinic acid from glucose with a molar yield of 24.9%. Activation of the synthetic bypass by the induced expression of Mycobacterium tuberculosis 2-ketoglutarate decarboxylase gene notably increased the yield of succinic acid. Functional activity of the synthetic bypass in the strain with the inactivated glyoxylate shunt and opened tricarboxylic acid cycle led to 2.7-fold increase in succinate yield from glucose. As the result, the substrate to the target product conversion reached 67.2%. The respective approach could be useful for the construction of the efficient microbial succinic acid producers.
      PubDate: 2018-05-01
      DOI: 10.1134/s0003683818030134
      Issue No: Vol. 54, No. 3 (2018)
       
  • Biodegradation of Gossypol by Mixed Fungal Cultures in Minimal Medium
    • Authors: V. Mageshwaran; V. Sharma; M. Chinnkar; N. Parvez; V. Krishnan
      Pages: 301 - 308
      Abstract: The fungal cultures, namely – Pleurotus sajor-caju MTCC 1806, Saccharomyces cerevisiae MTCC 6933 and Candida tropicalis MTCC 1406 and their combinations, C. tropicalis + S. cerevisiae, P. sajor-caju + S. cerevisiae and C. tropicalis + P. sajor-caju were grown in minimal medium containing 100 ppm of gossypol as the sole carbon and energy source. The culture supernatants of C. tropicalis + S. cerevisiae and P. sajor-caju + S. cerevisiae had low residual gossypol levels of 29 and 25 ppm, respectively. In the present study, we attempted to isolate gossypol-degrading enzyme and biodegraded gossypol from the culture supernatants of C. tropicalis + S. cerevisiae and P. sajor-caju + S. cerevisiae. The specific activity of laccase in the purified enzyme extracts of the C. tropicalis + S. cerevisiae and P. sajor-caju + S. cerevisiae treated samples was 425 and 224 U/mg, respectively. In SDS-PAGE, the gossypol-degrading enzyme was revealed as 3 bands of molecular weights ranging from 45 to 66 kDa. The characterization of biodegraded gossypol by FTIR analysis showed a reduction in aldehydes (C-H) stretches in samples treated with fungi. Mass spectrometry analysis revealed that the monoisotopic mass of the biodegraded gossypol was 474 g/mol.
      PubDate: 2018-05-01
      DOI: 10.1134/s0003683818030092
      Issue No: Vol. 54, No. 3 (2018)
       
  • Effect of 20-hydroxy-(5 Z ,8 Z ,11 Z ,14 Z )-eicosatetraenoic and
           Arachidonic Acids on Differentiation of Neurospora crassa
    • Authors: S. Yu. Filippovich; N. N. Gessler; G. P. Bachurina; E. A. Kharchenko; I. V. Ivanov; T. I. Sirotenko; A. B. Golovanov; T. A. Belozerskaya; N. V. Groza
      Pages: 309 - 315
      Abstract: Comparative analysis of the influence of arachidonic acid and its derivatives, 3-hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoic and 20-hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoic acids, on the formation of sexual and asexual reproductive structures in Neurospora crassa demonstrated that the presence and location of the hydroxyl group in unsaturated fatty acid determine the biological effect of the compound. In the presence of 5 μM of arachidonic acid, 20-HETE, and 3-HETE, vegetative spore formation in the light decreased by 45, 31, and 40%, respectively, indicating a similarity in the mechanisms of their action on the light-dependent asexual reproduction of the fungus. However, the effects of these compounds on the sexual process of N. crassa were radically different. Gas chromatography–mass spectrometry (GC–MS) revealed a fourfold increase in the monounsaturated oleic acid content in N. crassa cells after the addition of arachidonic acid. In the same time, the effect was not observed after the addition of 20-HETE. On the other hand, the increase in the linoleic acid content in the fungal mycelia was higher with 20-HETE.
      PubDate: 2018-05-01
      DOI: 10.1134/s0003683818030055
      Issue No: Vol. 54, No. 3 (2018)
       
  • Reactions of NADH Oxidation by Tetrazolium and Ubiquinone Catalyzed by
           Yeast Alcohol Dehydrogenase
    • Authors: K. A. Kamenskikh; N. L. Vekshin
      Pages: 316 - 319
      Abstract: The processes of NADH oxidation by p-NTF violet and ubiquinone catalyzed by isolated yeast alcohol dehydrogenase in aqueous and water-alcohol buffer solutions were studied. In the presence of p-NTF in aqueous solution at a pH of 6–7, NADH oxidation was extremely slow due to inhibition of the enzyme by the remaining enzyme-bound hydrophobic product, formazan, which forms during the reduction of p-Nitrotetrazolium. However, when the medium was alkalinized to a pH of 8–9 or when alcohol (ethanol or isopropanol) was added, formazan was desorbed from the enzyme, leading to an increase in the NADH oxidation rate. It was assumed that this redox reaction can be used as the basis for colorimetric measurement of the activity of different alcohol dehydrogenases. Tetrazolium reduction by alcohol was not observed at any value within the entire pH range. NADH oxidation in the presence of the enzyme and ubiquinone was also slow, even with the addition of alcohol, but its rate increased when the medium was acidified to a pH of 5.5–6. When a Tris-phosphate buffer was replaced with HEPES, a quasi-vibrational process was observed: NADH oxidization with ubiquinone to NAD+ and its subsequent reverse recovery with alcohol to NADH.
      PubDate: 2018-05-01
      DOI: 10.1134/s0003683818030067
      Issue No: Vol. 54, No. 3 (2018)
       
  • Proteolytic Activity of Chymotrypsin Immobilized on Selenium Nanoparticles
    • Abstract: Chymotrypsin was immobilized as a part of hybrid organo-inorganic nanocomplexes on selenium nanoparticles in the reduction reaction of selenious acid with ascorbic acid. Chymotrypsin stabilized the selenium nanoparticles in the solution. The spectral characteristics of synthesized nanocomplexes and the proteolytic activity of immobilized chymotrypsin were studied. It was shown that the immobilized enzyme had increased proteolytic activity in an alkaline medium.
      PubDate: 2018-07-01
       
  • Purification and Investigation of Physicochemical and Regulatory
           Properties of Homogeneous L-Lactate: Cytochrom c Oxidoreductase Obtained
           from the Nonsulfur Purple Bacterium Rhodovulum steppense
    • Abstract: L-Lactate: cytochrome c oxidoreductase activity was detected in cells of strain A-20s of the nonsulfur haloalkalophilic purple bacterium Rhodovulum steppense. An electrophoretically homogeneous preparation of the enzyme was obtained by purification. The enzyme had a specific activity of 4.75 U/mg protein, a 81.9-fold purification degree, and a 2.2% yield. The kinetic and physicochemical characteristics were determined. The value of the Michaelis constant with lactate was 15 μM. The temperature optimum for the studied enzyme was 31°C; optimum of pH was 8.2. It was found that the enzyme was a homodimer with a molecular weight of ~140 kDa; the mass of individual subunit was 68 kDa.
      PubDate: 2018-07-01
       
  • Expression in Plants of a Recombinant Protein Based on Flagellin Linked to
           Conservative Fragments of M2 Protein and Hemagglutintin of Influenza Virus
           
    • Abstract: The composition of traditional influenza vaccines require nearly annual updates due to the high variability of the influenza virus. The use of conservative viral antigens, the extracellular domain of the transmembrane protein M2, and fragments of the second subunit of hemagglutinin provides the opportunity to create recombinant broad-spectrum vaccines. Bacterial flagellin was used as a mucosal adjuvant to increase the immunogenicity of these conservative antigens. Recombinant proteins based on flagellin simultaneously containing M2e and a fragment of the hemagglutinin stem region were expressed in Nicotiana benthamiana plants using a self-replicating vector based on the potato virus X genome. Methods for their isolation from plants and purification have been developed. The developed expression system can be used to produce a new candidate influenza vaccine in plants.
      PubDate: 2018-07-01
       
  • Trends in Oil Production from Oleaginous Yeast Using Biomass:
           Biotechnological Potential and Constraints
    • Abstract: In the present scenario of depleting oil reservoir, microbial oil has gained much attention over plant and animal based sources. Among different microorganisms, yeast strains are considered superior source for oil production. The cost of oil produced by yeast could further be lowered using cheaper agro-waste and biomass as substrate. This review focuses on key topics which will help in gaining better understanding to enhance lipid production using yeast strains. The effects of oleaginous yeast co-culturing with microalgae, different cheap carbon sources of biomass, and types of yeast species on oil production were highlighted in the review. An overview of mechanisms of oil production from biomass, viz. pretreatment of biomass, fermentation and oil recovery are also provided. Constraints encountered during the oleogenesis or microbial oil accumulation and their probable solutions along with a section on different by-products obtained during oleo-genesis are also discussed.
      PubDate: 2018-07-01
       
  • Preparation and Characterization of Alphitobius diaperinus Melanin
    • Abstract: Melanin with a high antioxidant and sorption activity comparable to that of synthetic dioxyphenylalanine (DOPA)-melanin was isolated from the biomass of the darkling beetle Alphitobius diaperinus. The pigment was extracted with a solution of potassium hydroxide, followed by precipitation with concentrated hydrochloric acid and hydrolysis of the resulting precipitate with the same acid. The electron paramagnetic resonance (EPR) signal of melanin was characteristic of eumelanins with a spin concentration of 4.9 × 1017 spin per 1 g of dry weight. The melanin concentration that induced 50% inhibition of peroxidation was 9.2 μg/mL (the analogous concentration of DOPA-melanin was 8.0 μg/mL). The maximum of methylene-blue binding to the beetle melanin was 700 mg of dye per 1 g of dry weight of the preparation. The lipid-free melanin preparation exhibited antiradical activity.
      PubDate: 2018-07-01
       
  • Prospects for the Application of Yeast Display in Biotechnology and Cell
           Biology (Review)
    • Abstract: The technology of the yeast cell surface display, which appeared 20 years ago and was based on the displaying of target proteins on the cell surface via fusion to an abundant cell wall protein finds broad application in basic and applied research. The main advantage of the cell surface display on the basis of eukaryotic microorganisms—yeast—is the opportunity for correct modification of mammalian proteins. The cell surface display is an important tool for the analysis and understanding of protein function and protein–protein interactions and for the screening of novel clones from peptide and protein libraries. This technology makes it possible to obtain cells with novel abilities, such as catalytic functions and affinity binding to valuable ligands, including rare and heavy metals. It provides the chance to use yeast in biotechnology and in bioremediation and biomonitoring of the environment. The review considers the methods of obtaining a cell surface display on the basis of the yeasts Saccharomyces cerevisiae, Pichia pastoris, and Yarrowia lipolytica, the properties of anchor proteins, and the main fields of yeast display technology.
      PubDate: 2018-07-01
       
  • Bioregeneration of Leaching Solutions during Two-Step Processing of
           Copper-Zinc Concentrate
    • Abstract: A comparative study of the oxidation of ferrous iron ions by various cultures of acidophilic chemolithotrophic microorganisms in solutions obtained after ferric leaching of copper-zinc concentrate at 80°C has been carried out. It was shown that the use of a moderately thermophilic culture for bioregeneration of leaching solutions was preferable. At the same time, the oxidation rate of Fe2+ ions reached 0.88 g/(L h), or 21.1 g/(L day). We propose that the activity of the moderately thermophilic culture was due to the presence of the mixotrophic bacteria Sulfobacillus spp., which used organic products of the microbial lysis for their growth. These products were formed during high-temperature ferric leaching of the copper-zinc concentrate with the biosolution.
      PubDate: 2018-07-01
       
  • BSA Adsorption on Porous Scaffolds Prepared from BioPEGylated
           Poly(3-Hydroxybutyrate)
    • Abstract: Porous scaffolds for tissue engineering have been prepared from poly(3-hydroxybutyrate) (PHB) and a copolymer of poly(3-hydroxybutyrate) and polyethylene glycol (PHB-PEG) produced by bioPEGylation. The morphology of the scaffolds and their capacity for adsorption of the model protein bovine serum albumin (BSA) have been studied. Scaffolds produced from bioPEGylated PHB adsorbed more BSA, whereas the share of protein irreversibly adsorbed on these scaffolds was significantly lower (33%) than in the case of PHB homopolymer-based scaffolds (47%). The effect of protein adsorption on scaffold biocompatibility in vitro was tested in an experiment that involved the cultivation of fibroblasts (line COS-1) on the scaffolds. PHB-PEG scaffolds had a higher capacity for supporting cell growth than PHB-based scaffolds. Thus, the bioPEGylated PHB-based polymer scaffolds developed in the present study have considerable potential for use in soft tissue engineering.
      PubDate: 2018-07-01
       
  • Antimicrobial Peptaibols, Trichokonins, Inhibit Mycelial Growth and
           Sporulation and Induce Cell Apoptosis in the Pathogenic Fungus Botrytis
           cinerea
    • Abstract: Trichokonins (TKs) are antimicrobial peptaibols extracted from Trichoderma pseudokoningii strain SMF2. In this paper, it was discovered that TK VI, the main active ingredient of TKs, had a profound inhibitory effect on the growth and sporulation of the moth orchid gray mold, Botrytis cinerea. In addition, TK VI increased the cell membrane permeability of the pathogen. Further investigation of nuclear DNA fragmentation, subcellular structure disintegration, and mitochondrial membrane potential depolarization, as well as the appearance of reactive oxygen species, indicated that TK VI could induce programmed cell death in the necrotrophic pathogenic fungus B. cinerea.
      PubDate: 2018-07-01
       
  • Phytases and the Prospects for Their Application (Review)
    • Abstract: Phytases from plants and microorganisms release phospates from poorly soluble phytates, making the phospates more accessible. In this review, some features and biochemical properties of phytases, as well as the areas and prospects for their use, are under discussion. The introduction of phytases into the fodder for food-producing animals increases the product yield without any additional expenses for mineral phosphates. The presense of phytases in the soils reduces the risk of water eutrophication.
      PubDate: 2018-07-01
       
  • Highly Sensitive Immunochromatographic Assay for Qualitative and
           Quantitative Control of Beta-Agonist Ractopamine in Foods
    • Abstract: An immunochromatographic method has been developed for the determination of ractopamine, a low-molecular, nonsteroidal growth regulator controlled in food due to toxicity. The developed analysis was characterized by an instrument detection limit of 0.05 ng/mL, a working range of 0.07‒0.28 ng/mL, and a visual detection limit of 0.5 ng/mL, which meets the requirements of sanitary and hygienic control. It is shown that the developed immunochromatographic test systems can be used to test meat products.
      PubDate: 2018-07-01
       
  • Optimization of Metabolite Profiling for Black Medick ( Medicago lupulina
           ) and Peas ( Pisum sativum )
    • Abstract: Metabolic profiling is a key approach in current basic and applied research in biology. Comparative analysis of different metabolite extraction methods for pea (P. sativum) and black medick (M. lupulina) made it possible to find the optimal conditions for metabolite extraction and subsequent detection by gas chromatography coupled with mass spectrometry. The optimized method was shown to be reliable for assessment of the organ and species metabolic profiles for roots and leaves in pea and black medick plants.
      PubDate: 2018-07-01
       
  • Combined Effect of Salicylic Acid and Nitrogen Oxide Donor on
           Stress-Protective System of Wheat Plants under Drought Conditions
    • Abstract: Presowing treatment of wheat (Triticum aestivum L.) seeds with 10 or 100 μM salicylic acid (SA) reduced the inhibition of 14-day-old plant growth under soil drought. The same effect was caused by the spraying of 7-day-old seedlings with 0.5 or 2 mM nitrogen oxide donor (sodium nitroprusside, SNP) before drought. The protective effect was enhanced by the combination of seed treatment with 10 μM SA and plant spraying with 0.5 mM SNP, while their combinations in higher concentrations caused weaker effects. SA treatment in both concentrations and 0.5 mM SNP under drought conditions increased the antioxidant enzyme activity (superoxide dismutase, catalase, and guaiacol peroxidase) in leaves. This effect was especially significant when 10 μM SA was combined with 0.5 mM SNP. Spraying with 2 mM SNP and its combination with seed presowing with 100 μM SA did not significantly change the antioxidant enzyme activity; however, the proline content in the leaves increased. It is concluded that the SA stress-protective action on plants can be modified with exogenous nitrogen oxide.
      PubDate: 2018-07-01
       
  • The Role of Calcium-Dependent Protein Kinase Genes CPK16 , CPK25 , CPK30 ,
           and CPK32 in Stilbene Biosynthesis and the Stress Resistance of Grapevine
           Vitis amurensis Rupr.
    • Abstract: It is known that calcium-dependent protein kinases (CDPK or CPK) are implicated in the regulation of plant development and stress adaptation. However, there is a lack of information on the properties and functions of certain CDPK family members. The present study investigates the functions of four CDPK genes of the grapevine Vitis amurensis Rupr. in the formation of its high stress resistance level and the production of valuable secondary metabolites. Overexpression of the CPK30 gene of V. amurensis considerably increased the resistance of V. amurensis transgenic cell lines to salt and cold stresses, while CPK16, CPK25, and CPK32 overexpression did not influence the salt and temperature stress tolerance. VaCPK16 and VaCPK32 overexpression increased stilbene production in V. amurensis cell cultures by 2.1–3.1 and 1.6–3.1 times, respectively. The data indicate that the VaCPK30 gene is involved in the formation of grapevine salt and cold stress resistance, while the VaCPK16 and VaCPK32 genes contribute to increased stilbene accumulation.
      PubDate: 2018-07-01
       
  • Cloning, Isolation, and Properties of a New Homologous Exoarabinase from
           the Penicillium canescens Fungus
    • Abstract: A novel exo-arabinase (GH93, exo-ABN) enzyme produced by the ascomycete Penicillium canescens has been studied. Cloning of the abn1 gene coding for exo-ABN into the recipient P. canescens strain RN3-11-7 yielded recombinant producing strains characterized by a high yield of extracellular exo- ABN production (20–30% of the total amount of extracellular protein). Chromatographic purification yielded a homogenous exo-ABN with a molecular weight of 47 kDa, as shown by SDS-PAGE. The enzyme showed high specific activity towards linear arabinan (117 U/mg) and low specific activity towards branched arabinan and arabinoxylan (4–5 U/mg) and para-nitrophenyl-α-L-arabinofuranoside (0.3 U/mg), whereas arabinogalactan and para-nitrophenyl-α-L-arabinopyranoside, the substrates that contained the pyranose form of arabinose, were not hydrolyzed. Arabinohexaose was the major product of linear arabinan hydrolysis. Exo-ABN had a pH optimum at 5.0 and a temperature optimum at 60°C. The enzyme was stable in a broad pH range (4.0–7.0) and upon heating to 50°C during 180 min. Extensive hydrolysis of linear and branched arabinans by exo- and endo-arabinase mixtures, arabinofuranosidase, and arabinofuran-arabinoxylan hydrolase has been performed. The degree of substrate conversion amounted to 67 and 83% of the maximal possible value, respectively.
      PubDate: 2018-07-01
       
 
 
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