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 Agricultural ResearchJournal Prestige (SJR): 0.276 Citation Impact (citeScore): 1Number of Followers: 6      Hybrid journal (It can contain Open Access articles) ISSN (Print) 2249-720X - ISSN (Online) 2249-7218 Published by Springer-Verlag  [2352 journals]
• Varietal Response to Benzylaminopurine and Chlorocholine chloride on In
Vitro Tuberization of Potato
• Abstract: The potato varieties, Asterix, Granola and Diamant produce microtubers that are in high demand in Bangladesh. To achieve the full yield potentials of these varieties, an experiment was carried out to determine the efficient protocol for microtuberization. The results showed a wide range of variation in the response of the potato varieties to the addition of chlorocholine chloride [(2-chloroethyl)-trimethylammonium chloride] (CCC) and 6-benzylaminopurine (BAP) to the microtuberization medium. Potato varieties Asterix and Diamant were more efficient to microtuberization.
PubDate: 2019-03-18

• Microsatellite-based Genetic Diversity and Mutation-Drift Equilibrium in
Dharwadi Buffalo ( Bubalus bubalis ) of India
• Abstract: Genetic diversity was assessed in a buffalo population (Dharwadi) of Karnataka by utilizing 25 heterologous bovine microsatellite markers. Genotypic status of individuals at each locus was identified by automated DNA sequencer, and allelic data were analyzed for genetic diversity parameters. Observed number of alleles varied between 5 and 19 with 13.12 ± 0.80 alleles per locus. High level of heterozygosity (0.63) indicated plenty of existing genetic diversity in this buffalo population. Within-population inbreeding estimate (FIS) was significantly positive (0.186) and 88% of the investigated loci contributed toward the homozygote excess. Accordingly, significant deviation from Hardy–Weinberg equilibrium was observed at 24 of 25 analyzed loci. The population did not suffer reduction in effective population size in the last few generations. Assessment of Dharwadi buffalo population for mutation-drift equilibrium did not reveal significant heterozygosity excess under all the models of microsatellite evolution as per Sign test. No shift was recorded in the frequency distribution of alleles, and a normal L-shaped curve indicated absence of any recent genetic bottleneck. The study highlighted sufficient genetic variability within Dharwadi buffalo population that can be utilized in combination with phenotype and production information for registration as a distinct breed of Indian buffalo.
PubDate: 2019-03-18

• Biochemical Responses of Wheat Plants Primed with Ochrobactrum
pseudogrignonense and Subjected to Salinity Stress
• Abstract: A large number of bacteria were isolated from the rhizosphere of Imperata cylindrica, a facultative halophyte, and the most tolerant one with an ability to grow under 10% sodium chloride in vitro was selected and designated as IP8. This was identified to be Ochrobactrum pseudogrignonense on the basis of 16SrDNA analysis and showed positive plant-growth-promoting traits in vitro. Growth of wheat was enhanced by the bacterium under both non-stressed and salt-stressed conditions. Alleviation of salt stress by the bacterium was evident by the biochemical responses such as reduction in hydrogen peroxide accumulation as well as enhanced proline accumulation and antioxidative mechanisms. Activities of peroxidase, catalase, ascorbate peroxidase, and glutathione reductase were enhanced after salinity treatment, but that of superoxide dismutase was not. Treatment with IP8 and sodium chloride showed enhanced activities, and when compared to sodium chloride alone, peroxidase showed further up-regulation in some cultivars and superoxide dismutase also showed significantly increased activities. Salinity had no effect on activities of defense enzymes in general. Activities of defense enzymes were significantly enhanced by IP8 alone or in addition to salinity treatment. Microarray analysis revealed that 282 genes were down-regulated and 6022 up-regulated after application of O. pseudogrignonense under salt stress induction by 200 mM sodium chloride. Among the up-regulated genes were those of peroxidase, phenylalanine ammonia lyase, chitinase, glucanase, as well as germin-like proteins, histone H2B, and sulfur-rich thionin-like proteins. Genes for ascorbate peroxidase, lipid transfer proteins, and salt stress responsiveness were among the down-regulated ones.
PubDate: 2019-03-18

• Variation in Nutritional and Shelf Life Parameters Among Rabi Sorghum
Cultivars and Effect of Processing on These Parameters
• Abstract: Variation in grain mineral contents, proximate composition, shelf stability parameters, and vitamins of eight rabi grain sorghum cultivars and effect of processing (dehulling and parboiling) on these parameters were investigated. Wide variations were observed among the rabi cultivars tested for different nutritional and shelf stability parameters. Parboiling process resulted in increase of grain calcium and zinc. Dehulling resulted in significant loss in all grain minerals tested. Grain proximate compositions such as carbohydrate, proteins and vitamins like thiamine, niacin, and folic acid also showed significant increase in the parboiled-dehulled sorghum compared to raw sorghum, indicating the possible nutritional benefits that can be exploited from parboiling in sorghum. Alcoholic acidity, an important parameter to assess the shelf stability of grain, gradually increased from 0.08 and reached 0.18 at 90th day of storage period, giving indication that grain samples can be stored for 90 days without much loss due to deterioration. Alcoholic acidity of parboiled grains were in a range between 0.07 and 0.08 even at 90th day of storage period indicating positive role of parboiling in improving shelf stability of sorghum grains. Water activity for raw and parboiled sorghum for both raw and parboiled sorghum was within the limits at the 90th day of storage period.
PubDate: 2019-03-18

• Finger Millet in Tribal Farming Systems Contributes to Increased
Availability of Nutritious Food at Household Level: Insights from India
• Abstract: The challenge to food production posed by climate aberrations has been seeing increased attention to reviving millet-based farming systems. Millets are climate-resilient and nutritionally equivalent or superior to most other cereals, making them a favourable crop to address the prevalence of malnutrition. Finger millet (Eleusine coracana) is among the major crops cultivated in the undulating terrains of Koraput district of Odisha, India. It is consumed as a staple food and drink by the local tribal communities. However, over the years there has been rapid decline both in area and in production of the crop leading to reduced grain availability for household consumption. With a view to increase the productivity, the study assessed the effects of possible combinations of crop varieties and agronomic practices that can be customized for finger millet production system in Koraput over 2 years (2015–2017). The study focused on crop productivity, profitability and labour requirement along with nutrition awareness initiatives. On-farm trial with improved variety ‘GPU-67’ with line transplanting and recommended fertilizer management in 2015–2016 showed 31 and 50% higher grain yield and profit than that of farmers’ practice (1579 kg ha− 1 and ₹13,730 ha− 1, respectively) and was counted as a recommended cultivation package. In 2016–2017, the recommended practice showed 60% higher grain yield and 1.16 times more profit than farmers’ practice (1575 kg ha− 1 and ₹14,000 ha− 1, respectively) (P < 0.000). Both total and women’s labour requirement per ha was lower under recommended practice. An endline survey in 2017 revealed improved household consumption over baseline.
PubDate: 2019-03-07

• Antioxidant and Antimicrobial Efficacy of Peptidic Hydrolysate Obtained
from Porcine Blood
• Abstract: Aim of present research was to explore changes in pH, degree of hydrolysis (DH) antioxidant [2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 2,2′-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing-antioxidant power assay (FRAP)] and antibacterial property of enzymatic hydrolysates obtained from hydrolysis of porcine blood using proteases viz., alcalase, trypsin and papain. It was observed that pH of porcine blood hydrolysates slightly decreased as hydrolysis progressed for all the enzymes. DH improved considerably (p < 0.05) up to 6 h for alcalase and papain digested samples, while it increased significantly (p < 0.05) up to 4 h for trypsin. ABTS, DPPH, FRAP assay and antimicrobial activity improved appreciably (p < 0.05) indicating positive correlation with duration of digestion and DH. SDS-PAGE showed distinct decrease in amount and intensity of protein bands with progressing duration of digestion. It can be concluded from the study that porcine blood can be utilized as excellent substrate for production of protein hydrolysates with potent antioxidant and antimicrobial activities which may be employed for meat/food products preservation.
PubDate: 2019-03-01

• Disinfestation of Chickpea and Green Gram from Callosobruchus maculatus
Adults Through Hot-Air-Assisted Microwave Heating System
• Abstract: Pulse bruchid-infested samples of green gram (Vigna radiata) and chickpea (Cicer arietinum) having different moisture contents (7.5–9.5% wb) (1 cm bed depth) were exposed to microwave–hot air treatment for different exposure times (3 min 20 s to 6 min) and hot air temperatures (40–60 °C). Protein content, cooking time, textural kinetics, color and viability of the treated samples were analyzed, and techno-economic feasibility analysis was carried out for the process. Hundred percent mortality of adult insect (Callosobruchus maculatus) was achieved immediately, at an air temperature of 60 °C with microwave exposure period of 6 min at 2900 W power level. All the three processing parameters, i.e., moisture content, period of exposure and hot air temperature, were found to have significant effect on the mortality of pulse bruchids for both chickpea and green gram. Negligible differences were observed between the control and treated samples with respect to protein content for both chickpea and green gram. Cooking time, textural hardness, L value, greenness and viability of treated samples were lower than control sample. Cost of the treatment works out to be INR 6.28–7.07 per kg which is likely to come down with economics of scale.
PubDate: 2019-03-01

• Temporal and Vertical Variation of Selected Extracellular Enzyme
Activities on Tree Litter Degradation of a Subtropical Forest
• Abstract: The present study focused on the soil enzyme activities through in situ incubation of litter decomposition pits across major seasons in a subtropical forest of Bengal. This study examined the activities of extracellular enzymes (amylase, cellulase and invertase) and their relationship with the types of litter decay and soil physiochemical properties at different depths of the soil matrix. The higher decay constant (k) of decomposed leaf litter was recorded for Tectona and lower for Albizia in the studied three seasons. The results also revealed that the activities of soil extracellular enzymes were higher in rainy season compared to winter in all experimental sites with respect to control. Among the studied enzymes, invertase was significantly higher in different decomposed sites at the surface soils compared to other enzymes during the rainy season. The results also demonstrated that the enzyme activities gradually declined from the surface soil to subsurface soil at the different decomposed sites. However, in surface soil, the amylase and invertase activities were highest in Tectona litter-containing soil and cellulase activity was highest in Shorea litter-containing soil in all the studied seasons. On the other hand, soil parameters such as moisture, organic carbon and available nitrogen were significantly correlated with enzyme activities in different decomposed sites. The results suggest that the activity of the extracellular enzymes involved in litter decomposition varies in different species and seasons.
PubDate: 2019-03-01

• Diversity and Antifungal Activity of Fungal Endophytes of Asparagus
racemosus Willd
• Abstract: Endophytic microbes are hosted inside plants in a symbiotic and hugely benefitting relationship. In the present work, 60 asymptomatic fungi representing nine different genera were isolated from 286 plant tissues of Asparagus racemosus. Fungal endophytes were identified by ITS rDNA sequencing. Penicillium sp. was the most dominant fungus. Tissue specificity was observed by principal component analysis. Cluster analysis revealed correlation between fungal species abundance and mean temperature. Highest Shannon diversity was recorded in leaf tissues ( $${H^\prime }$$  = 1.279) from Delhi in 2010. Pielou’s evenness index was highest in stem tissues sampled from Hyderabad in the first survey. Of the total number of isolates examined, 12% of fungal endophytes demonstrated antifungal activity against the causal agents of four distinctive plant diseases (grey mould, stem rot, root rot and wilting, i.e. Botrytis cinerea, Sclerotinia sclerotiorum, Rhizoctonia solani and Fusarium oxysporum, respectively) in dual-culture bioassay. Penicillium sp. (isolate ARDS-2.3) and Aspergillus oryzae (isolate ARHS-1.1) displayed most effective antifungal activity with IC50 value ranging from 0.381 to 0.955 mg/ml against the broad-spectrum phytopathogens investigated.
PubDate: 2019-03-01

• Soil Class, Mechanical Impedance and Irrigation: Impact on Physiological
Performance in Green Dwarf Coconut
• Abstract: The objective of this study was to assess the physiological aspects of young green dwarf coconut cultivated in greenhouse as a function of soil class and different compaction levels and moisture conditions. The experiment was carried out in a greenhouse, using a randomized complete block design with nine replications and with 32 treatments in a 2 × 4 × 4 factorial scheme, with two soil classes—Typic Kandiudult and Umbric Dystrochrept, four compaction levels and four soil moisture conditions. Leaf predawn water potential (Ψw), net photosynthetic (A), stomatal conductance (gs), transpiration rates (E) and growth traits were higher in coconut grown in Umbric Dystrochrept soil. Moreover, these plants showed the highest values of maximum quantum yield of photosystem II (Fv/Fm) and leaf ‘greenness' (SPAD reading) was higher, but only in the lowest soil moisture condition, when compared with Typic Kandiudult. Higher soil moisture led to a higher Ψw for both soil classes. Close relationship between SPAD reading and the increase in soil moisture as well as between Fv/Fm and the increase in soil moisture for coconuts grown in Typic Kandiudult soil was observed. Furthermore, good correlations were observed between Ψw and the increase in soil moisture in coconuts grown in both soil classes as well as between Fv/Fm and the increase in soil density in coconuts grown in Typic Kandiudult soil. Effects of soil compaction were not observed on other physiological variables. Overall, green dwarf coconut trees grown in Umbric Dystrochrept soil allowed the higher water storage, which may contribute to increases in circumference, plant height, leaf area and dry weight (leaves, stem and root) linked to high photosynthetic rates.
PubDate: 2019-03-01

• Crop Establishment Methods, Use of Microbial Consortia, Biofilms and Zinc
Fertilization for Enhancing Productivity and Profitability of Rice–Wheat
Cropping System
• Abstract: Rice–wheat cropping system in India (RWCS) is facing several problems which lead to reducing productivity and profitability. Change in the crop establishment methods (CEMs) and nutrient and water management strategies have considerable influence on both productivity and profitability. Our 2-year study at ICAR—Indian Agricultural Research Institute—showed that net return in aerobic rice system followed by (fb) zero-till wheat increased by ₹ 3416–7737 ha−1 over the conventional puddled transplanted rice fb conventional drill-sown wheat and ₹ 7864–12,376 ha−1 over system of rice intensification (SRI) fb system of wheat intensification due to savings in energy and water, even though gross return and system productivity were statistically identical in all three CEMs. Zinc fertilization increased the net return and system productivity by ₹ 5350–7670 ha−1 and 0.34–0.39 Mg ha−1 when applied with 100% recommended dose of fertilizer (120 kg nitrogen ha−1 and 25.8 kg phosphorus ha−1). Nutrient management strategy involving Anabaena–Pseudomonas biofilmed formulations improved system productivity by 350–440 kg ha−1 and net return by ₹ 9217–11,344 ha−1 over the same quantity of chemical fertilizer application. Net return was highly correlated with cost of cultivation (R2 = 0.95 and 0.99) than the gross return in both years indicating the importance of cost of cultivation in selecting CEM than gross return.
PubDate: 2019-03-01

• Improving Global Hunger Index
• Abstract: End of hunger is one of the United Nations Sustainable Development Goals. Proper assessment of hunger is of great assistance to planners and administrators. The paper suggests ways to improve the currently used Global Hunger Index (GHI), which describes the hunger situation in different countries. GHI measures the prevalence of some broad outcomes of hunger like undernutrition and mortality-related proxy indicators. A limitation of GHI is the upward bias it induces because of using proxy indicators involving undernutrition and under-5 mortality. This is because while hunger leads to undernutrition, hunger alone is not the only reason for undernutrition or mortality. Use of other indicators having a direct bearing on hunger like dietary intake and behavioral responses on food access is suggested. The search for an improved hunger estimate requires computer-based statistical techniques like simulation. The use of all these indicators is likely to improve the hunger estimate.
PubDate: 2019-03-01

• Identification of Protein Motifs in Phytoplasma Associated with Root
(Wilt) Disease of Coconut ( Cocos nucifera ) Using an Improved Statistical
Measure
• Abstract: Protein motif is a conserved short region within larger sequence, and it provides an important way to get functional or structural information about a noble protein sequence. This study primarily provides a new approach to identify motifs which are considered to play an important role to determine functional or structural class of any protein. Coconut palm (Cocos nucifera L.) root (wilt) disease (CRWD) became a serious concern for coconut cultivation in the coastal region of South India. It has already been reported that species of the phytoplasma (-belonging to ribosomal group 16SrXI) is primarily associated with CRWD. In this study, our objective is to modify an existing motif identification algorithm, and also identify motifs that describe their conserved region within a CRWD phytoplasma protein sequence. Accordingly, the existing information content measure formulas for a no-gapped and gapped aligned sequence set were re-formulated. Further, an improvement on both these existing measures has been vied by incorporating prior information of BLOSUM90 substitution matrix. Results showed that proposed modifications could successfully identify N-terminal myristoylation motif within an alignment matrix of Arabidopsis thaliana sequences; concurrently our modification has also been validated. Both the approaches, namely frequency- and BLOSUM90-based information content, have been compared using the observed results, in terms of identified motifs. Finally, we could also test this noble BLOSUM90-based approach to correctly predict conserved regions at locations, (163–171) and (117–138), in an aligned secA gene sequence set of phytoplasma, an etiological agent of CRWD.
PubDate: 2019-03-01

• Antioxidant Potential of Indian Eggplant: Comparison Among White, Purple
and Green Genotypes Using Chemometrics
• Abstract: Twenty-six eggplant genotypes of different colours were evaluated for their variations in total phenolics, total flavonoids, total monomeric anthocyanin and antioxidant activity. Antioxidant potentiality was determined using two in vitro assays, viz. cupric ion reducing antioxidant capacity and 2, 2-diphenyl-1-picrylhydrazyl (DPPH). Additionally, five colour attributes were evaluated. Among the eggplant genotypes, significant differences (p < 0.05) were observed with respect to antioxidant composition and antioxidant activity. Total phenolics and total flavonoids varied from 13.00 to 49.29 mg/100 g fresh weight (FW) and 5.30 to 28.70 mg/100 g FW, respectively. Chemometric tools such as principal component analysis (PCA) and agglomerative hierarchical clustering (AHC) were used to understand the possible classification of Indian eggplant genotypes based on their colour properties, antioxidant composition and antioxidant potentiality. PCA revealed that the first two components represented 80.68% of the total variability in the total variation. AHC classified genotypes into three main groups on the basis of the measured parameters. A white-coloured genotype was found to be the richer source of phenolics and flavonoids with high antioxidant activity.
PubDate: 2019-03-01

• Phenotypic Diversity of Rice Landraces Collected from Goa State for
Salinity and Agro-morphological Traits
• Abstract: The present study aimed at the characterization of rice landraces collected from the state of Goa for their utilization in breeding programs. Eighteen landraces of rice were collected from the remote villages of Goa during the survey conducted between 2010 and 2013 along with passport data and traditional knowledge. All the 18 landraces were characterized for 27 qualitative and 16 quantitative characters as per the DUS guidelines. They were also screened for salt tolerance at seedling stage under microplot conditions. Qualitative characters were analyzed using Shannon diversity and Cluster analysis, whereas quantitative characters were subjected to principal component analysis (PCA). Shannon diversity index ranged from 0.00 to 1.00 with a mean of 0.60. Cluster analysis grouped the accessions into two major clusters with three subclusters in each of the main cluster with a Jaccard similarity coefficient varying between 0.38 and 0.87. PCA has revealed four major components (eigenvalue > 1), which altogether explained 80.60% of the total variation. Phenotyping for salt stress at seedling stage grouped the landraces as highly tolerant (Korgut-red), moderately tolerant (Shidde, Korgut-black) and sensitive to highly sensitive. Information generated from this study would be useful in selecting landrace collections as donors in breeding new varieties.
PubDate: 2019-03-01

• Development, Testing and Optimization of a Screw Press Oil Expeller for
Moringa ( Moringa oleifera ) Seeds
• Abstract: A screw press moringa oil expeller was developed and evaluated in terms of oil expression efficiency (OEE), material balance efficiency (MBE) and expression loss (EL). Four different models were fitted to the output variables. Maximum OEE of 81.66% was obtained at moisture content of 11% wet basis, heating temperature of 80 °C, heating time of 30 min and applied pressure of 20 MPa. The coefficient of determination (R2) for the OEE was 0.77. Predicted optimum OEE of 80.62% at moisture content of 11.30% wet basis, temperature of 85.55 °C, time of 27.17 min and pressure of 19.64 MPa was obtained with a desirability of 0.867. Under these optimal conditions, the experimental value was 80.74%. Deviations between experimental and predicted values were low and statistically insignificant which implies the model chosen can effectively predict the OEE. Maximum MBE and EL were 95.47 and 4.53%, respectively, which shows a better conversion of input (seeds) to output (oil and cake) materials with minimal losses along the production line. However, it was observed that the mean is a better predictor for the MBE and EL than any of the models considered as the experimental values obtained were very close and statistically insignificant.
PubDate: 2019-03-01

• The Anti-malnutrition Effect of Fulvic Acid from Yunnan Young Brown Coal
in Silkworms
• Abstract: Mulberry powdery mildew often leads to a severe loss in cocoon production during the late autumn silkworm season. We investigated if fulvic acid sodium (FANa) from Yunnan young brown coal could reduce such losses. We examined the effects of FANa on 9 types of bacteria isolated from diseased silkworms, and we examined its effects on starvation resistance and high-temperature survival of silkworms. The results indicated that FANa had no noticeable influence on bacteria, but it significantly increased survival of silkworms during fasting and in high-temperature conditions (increased 12.21 and 24.16%, respectively, p < 0.05). Finally, we evaluated if FANa could reduce malnutrition of silkworms caused by diseased mulberry leaf ingestion and tested it in a mulberry-reducing model. Compared to the control group, the model group (given 80% of leaves) significantly delayed the spinning time (3 days, p < 0.0001), decreased cocoon weight (28% less, p < 0.05) and reduced cocoon production (41% less, p < 0.0001). However, addition of 4% FANa shortened the larval stage (2 days, p < 0.01), and it saved 27% of leaves and 22% of work time. Moreover, FANa increased the cocoon weight (101% of control, p < 0.05) and increased cocoon production (79% of control, p < 0.05) compared to the model group. Taken together, these results suggest that FANa can improve resistance to malnutrition in silkworms and reduce the loss caused by mulberry powdery mildew.
PubDate: 2019-03-01

• Sheep and Goat Corralling Density Effect on Soil Properties and Weed
Species Diversity of Arable Lands
• Abstract: The hypothesis that livestock corralling improves soil properties and weed species richness was investigated in a 2-year on-farm trial conducted in Sudan savanna agro-ecology of Ghana. The effect of three stocking densities of sheep and goats corralling (0, 70 and 140 head ha−1) on soil properties and weed species diversity was evaluated in a randomized complete block design with eight replicates. Sheep and goats weighing 27 ± 2.0 and 24 ± 1.5 kg, respectively, were corralled on fallow arable lands from 19: 00 to 06: 00 GMT hours daily for 178 nights during 2014 and 2015 cropping seasons. Soil parameters measured include pH, organic carbon (OC), total nitrogen, available phosphorus, exchangeable potassium, microbial biomass carbon, microbial biomass nitrogen, soil microbial quotient, earthworm cast, bulk density, porosity, and moisture. Soil properties and weed species diversity increased (P < 0.01) with sheep and goats corralling relative to the control. Broadleaf and sedge species were positively correlated with soil OC while the grass was positively correlated with soil OC, microbial biomass carbon, bulk density, and porosity. The results suggest that crop-livestock farmers could corral 70 head ha−1 sheep and goats for soil fertility amendment on Ferric Lixisol in Sudan savanna agro-ecology of West Africa and similar agro-ecologies.
PubDate: 2019-03-01

• Molecular Verification of Putative Zygotic Seedlings in Different
Intra-Specific Crosses in Mandarins ( Citrus reticulata ) by SSR Markers
• Abstract: The molecular verification of different intra-specific mandarin hybrids was explored during 2014–2015. Simple sequence repeat (SSR) markers were employed to identify putative zygotic seedlings from hybrid progeny produced from different crosses in mandarin. The most informative SSR marker in the F1 hybrids of ‘Daisy × Kinnow’ cross was CCSM170 which identified 73% of zygotic seedlings followed by CMS4, AG14, CiBE6006 and CiBE3397 which identified 38.5%, 31.5%, 27% and 24% of zygotic seedlings, respectively. Most efficient SSR marker in ‘Kinnow × Daisy’ cross was CiBE6006 which identified 22.5% zygotic seedlings followed by CCSM170, CiBE3397 and CMS4 which identified 17.5%, 12.5% and 5.5% of zygotic seedlings, respectively. In ‘W. Murcott × Kinnow’ hybridization, CMS31 marker identified 28.5% zygotic seedlings in F1 progeny followed by CiBE3298 which identified 14.5% zygotic seedlings. The SSR marker CiBE3397 identified 19.5% zygotic seedlings in ‘Kinnow × W. Murcott’ cross. The results show that the SSR markers can differentiate the zygotic seedlings of F1 progeny in different intra-specific crosses in mandarins.
PubDate: 2019-03-01

• Identification of Rhizosphere Bacterial Diversity with Promising Salt
Tolerance, PGP Traits and Their Exploitation for Seed Germination
Enhancement in Sodic Soil
• Abstract: The present research was carried to explore bacterial diversity in rhizosphere of salt tolerant rice and wheat genotypes cultivated in sodic soils and examined for the plant growth promoting traits, seed germination and vigor index of wheat in salt affected soils of pH > 9.30. Soil was collected and analyzed for physico-chemical properties using standard methods. Valuable bacterial population was isolated from the rhizosphere of salt tolerant rice and wheat varieties grown in salt affected sodic soils of Uttar Pradesh, India. Isolates were tested for PGP traits, NaCl uptake and tolerance at varying concentration (0.5, 5.0, 7.5, 10.0%). Bacterial isolates possessing high salt tolerance and PGP traits were selected for molecular identification and further exploited for the seed germination in wheat. On the basis of partial sequencing of 16S rRNA gene in nine potential isolates were confirmed as Pseudomonas sp., Agromyces tropicus, Lysinibacillus fusiformis, Bacillus flexus, Lysinibacillus sp., Bacillus tequilensis, Bacillus licheniformis, Pseudomonas mendocina and Bacillus licheniformis, respectively. The Lysinibacillus sp. was most effective in terms of improving seed germination percent (83%), shoot length (20.3 cm), fresh (0.41 g) and dry weight (0.057 g), and seedlings vigor index I (2056.2) and II (6.2). On the other side, root growth (length, fresh and dry weight) was found significantly higher with the inoculation of Lysinibacillus fusiformis. Salt tolerant PGPR of this study could be useful for the reclamation of saline/sodic soils concomitant with improved plant growth and yield.
PubDate: 2019-03-01

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