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Publisher: Springer-Verlag (Total: 2352 journals)

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Showing 1 - 200 of 2352 Journals sorted alphabetically
3D Printing in Medicine     Open Access   (Followers: 2)
3D Research     Hybrid Journal   (Followers: 21, SJR: 0.222, CiteScore: 1)
4OR: A Quarterly J. of Operations Research     Hybrid Journal   (Followers: 10, SJR: 0.825, CiteScore: 1)
AAPS J.     Hybrid Journal   (Followers: 23, SJR: 1.118, CiteScore: 4)
AAPS PharmSciTech     Hybrid Journal   (Followers: 7, SJR: 0.752, CiteScore: 3)
Abdominal Imaging     Hybrid Journal   (Followers: 16, SJR: 0.866, CiteScore: 2)
Abhandlungen aus dem Mathematischen Seminar der Universitat Hamburg     Hybrid Journal   (Followers: 4, SJR: 0.439, CiteScore: 0)
Academic Psychiatry     Full-text available via subscription   (Followers: 26, SJR: 0.53, CiteScore: 1)
Academic Questions     Hybrid Journal   (Followers: 8, SJR: 0.106, CiteScore: 0)
Accreditation and Quality Assurance: J. for Quality, Comparability and Reliability in Chemical Measurement     Hybrid Journal   (Followers: 29, SJR: 0.316, CiteScore: 1)
Acoustical Physics     Hybrid Journal   (Followers: 11, SJR: 0.359, CiteScore: 1)
Acoustics Australia     Hybrid Journal   (SJR: 0.232, CiteScore: 1)
Acta Analytica     Hybrid Journal   (Followers: 7, SJR: 0.367, CiteScore: 0)
Acta Applicandae Mathematicae     Hybrid Journal   (Followers: 1, SJR: 0.675, CiteScore: 1)
Acta Biotheoretica     Hybrid Journal   (Followers: 4, SJR: 0.284, CiteScore: 1)
Acta Diabetologica     Hybrid Journal   (Followers: 19, SJR: 1.587, CiteScore: 3)
Acta Endoscopica     Hybrid Journal   (Followers: 1)
acta ethologica     Hybrid Journal   (Followers: 4, SJR: 0.769, CiteScore: 1)
Acta Geochimica     Hybrid Journal   (Followers: 7, SJR: 0.24, CiteScore: 1)
Acta Geodaetica et Geophysica     Hybrid Journal   (Followers: 3, SJR: 0.305, CiteScore: 1)
Acta Geophysica     Hybrid Journal   (Followers: 11, SJR: 0.312, CiteScore: 1)
Acta Geotechnica     Hybrid Journal   (Followers: 7, SJR: 1.588, CiteScore: 3)
Acta Informatica     Hybrid Journal   (Followers: 5, SJR: 0.517, CiteScore: 1)
Acta Mathematica     Hybrid Journal   (Followers: 13, SJR: 7.066, CiteScore: 3)
Acta Mathematica Hungarica     Hybrid Journal   (Followers: 2, SJR: 0.452, CiteScore: 1)
Acta Mathematica Sinica, English Series     Hybrid Journal   (Followers: 6, SJR: 0.379, CiteScore: 1)
Acta Mathematica Vietnamica     Hybrid Journal   (SJR: 0.27, CiteScore: 0)
Acta Mathematicae Applicatae Sinica, English Series     Hybrid Journal   (SJR: 0.208, CiteScore: 0)
Acta Mechanica     Hybrid Journal   (Followers: 21, SJR: 1.04, CiteScore: 2)
Acta Mechanica Sinica     Hybrid Journal   (Followers: 5, SJR: 0.607, CiteScore: 2)
Acta Metallurgica Sinica (English Letters)     Hybrid Journal   (Followers: 7, SJR: 0.576, CiteScore: 2)
Acta Meteorologica Sinica     Hybrid Journal   (Followers: 3, SJR: 0.638, CiteScore: 1)
Acta Neurochirurgica     Hybrid Journal   (Followers: 7, SJR: 0.822, CiteScore: 2)
Acta Neurologica Belgica     Hybrid Journal   (Followers: 1, SJR: 0.376, CiteScore: 1)
Acta Neuropathologica     Hybrid Journal   (Followers: 4, SJR: 7.589, CiteScore: 12)
Acta Oceanologica Sinica     Hybrid Journal   (Followers: 3, SJR: 0.334, CiteScore: 1)
Acta Physiologiae Plantarum     Hybrid Journal   (Followers: 3, SJR: 0.574, CiteScore: 2)
Acta Politica     Hybrid Journal   (Followers: 15, SJR: 0.605, CiteScore: 1)
Activitas Nervosa Superior     Hybrid Journal   (SJR: 0.147, CiteScore: 0)
adhäsion KLEBEN & DICHTEN     Hybrid Journal   (Followers: 8, SJR: 0.103, CiteScore: 0)
ADHD Attention Deficit and Hyperactivity Disorders     Hybrid Journal   (Followers: 25, SJR: 0.72, CiteScore: 2)
Adhesion Adhesives & Sealants     Hybrid Journal   (Followers: 9)
Administration and Policy in Mental Health and Mental Health Services Research     Partially Free   (Followers: 17, SJR: 1.005, CiteScore: 2)
Adsorption     Hybrid Journal   (Followers: 4, SJR: 0.703, CiteScore: 2)
Advances in Applied Clifford Algebras     Hybrid Journal   (Followers: 4, SJR: 0.698, CiteScore: 1)
Advances in Atmospheric Sciences     Hybrid Journal   (Followers: 37, SJR: 0.956, CiteScore: 2)
Advances in Computational Mathematics     Hybrid Journal   (Followers: 19, SJR: 0.812, CiteScore: 1)
Advances in Contraception     Hybrid Journal   (Followers: 3)
Advances in Data Analysis and Classification     Hybrid Journal   (Followers: 59, SJR: 1.09, CiteScore: 1)
Advances in Gerontology     Partially Free   (Followers: 8, SJR: 0.144, CiteScore: 0)
Advances in Health Sciences Education     Hybrid Journal   (Followers: 30, SJR: 1.64, CiteScore: 2)
Advances in Manufacturing     Hybrid Journal   (Followers: 4, SJR: 0.475, CiteScore: 2)
Advances in Polymer Science     Hybrid Journal   (Followers: 45, SJR: 1.04, CiteScore: 3)
Advances in Therapy     Hybrid Journal   (Followers: 5, SJR: 1.075, CiteScore: 3)
Aegean Review of the Law of the Sea and Maritime Law     Hybrid Journal   (Followers: 6)
Aequationes Mathematicae     Hybrid Journal   (Followers: 2, SJR: 0.517, CiteScore: 1)
Aerobiologia     Hybrid Journal   (Followers: 3, SJR: 0.673, CiteScore: 2)
Aesthetic Plastic Surgery     Hybrid Journal   (Followers: 11, SJR: 0.825, CiteScore: 1)
African Archaeological Review     Hybrid Journal   (Followers: 20, SJR: 0.862, CiteScore: 1)
Afrika Matematika     Hybrid Journal   (Followers: 1, SJR: 0.235, CiteScore: 0)
AGE     Hybrid Journal   (Followers: 7)
Ageing Intl.     Hybrid Journal   (Followers: 7, SJR: 0.39, CiteScore: 1)
Aggiornamenti CIO     Hybrid Journal   (Followers: 1)
Aging Clinical and Experimental Research     Hybrid Journal   (Followers: 3, SJR: 0.67, CiteScore: 2)
Agricultural Research     Hybrid Journal   (Followers: 6, SJR: 0.276, CiteScore: 1)
Agriculture and Human Values     Hybrid Journal   (Followers: 14, SJR: 1.173, CiteScore: 3)
Agroforestry Systems     Hybrid Journal   (Followers: 20, SJR: 0.663, CiteScore: 1)
Agronomy for Sustainable Development     Hybrid Journal   (Followers: 13, SJR: 1.864, CiteScore: 6)
AI & Society     Hybrid Journal   (Followers: 9, SJR: 0.227, CiteScore: 1)
AIDS and Behavior     Hybrid Journal   (Followers: 14, SJR: 1.792, CiteScore: 3)
Air Quality, Atmosphere & Health     Hybrid Journal   (Followers: 4, SJR: 0.862, CiteScore: 3)
Akupunktur & Aurikulomedizin     Full-text available via subscription   (Followers: 1)
Algebra and Logic     Hybrid Journal   (Followers: 6, SJR: 0.531, CiteScore: 0)
Algebra Universalis     Hybrid Journal   (Followers: 2, SJR: 0.583, CiteScore: 1)
Algebras and Representation Theory     Hybrid Journal   (Followers: 1, SJR: 1.095, CiteScore: 1)
Algorithmica     Hybrid Journal   (Followers: 9, SJR: 0.56, CiteScore: 1)
Allergo J.     Full-text available via subscription   (Followers: 1, SJR: 0.234, CiteScore: 0)
Allergo J. Intl.     Hybrid Journal   (Followers: 2)
Alpine Botany     Hybrid Journal   (Followers: 5, SJR: 1.11, CiteScore: 3)
ALTEX : Alternatives to Animal Experimentation     Open Access   (Followers: 3)
AMBIO     Hybrid Journal   (Followers: 10, SJR: 1.569, CiteScore: 4)
American J. of Cardiovascular Drugs     Hybrid Journal   (Followers: 16, SJR: 0.951, CiteScore: 3)
American J. of Community Psychology     Hybrid Journal   (Followers: 29, SJR: 1.329, CiteScore: 2)
American J. of Criminal Justice     Hybrid Journal   (Followers: 9, SJR: 0.772, CiteScore: 1)
American J. of Cultural Sociology     Hybrid Journal   (Followers: 17, SJR: 0.46, CiteScore: 1)
American J. of Dance Therapy     Hybrid Journal   (Followers: 4, SJR: 0.181, CiteScore: 0)
American J. of Potato Research     Hybrid Journal   (Followers: 2, SJR: 0.611, CiteScore: 1)
American J. of Psychoanalysis     Hybrid Journal   (Followers: 21, SJR: 0.314, CiteScore: 0)
American Sociologist     Hybrid Journal   (Followers: 14, SJR: 0.35, CiteScore: 0)
Amino Acids     Hybrid Journal   (Followers: 8, SJR: 1.135, CiteScore: 3)
AMS Review     Partially Free   (Followers: 4)
Analog Integrated Circuits and Signal Processing     Hybrid Journal   (Followers: 7, SJR: 0.211, CiteScore: 1)
Analysis and Mathematical Physics     Hybrid Journal   (Followers: 5, SJR: 0.536, CiteScore: 1)
Analysis in Theory and Applications     Hybrid Journal   (Followers: 1)
Analysis of Verbal Behavior     Hybrid Journal   (Followers: 6)
Analytical and Bioanalytical Chemistry     Hybrid Journal   (Followers: 32, SJR: 0.978, CiteScore: 3)
Anatomical Science Intl.     Hybrid Journal   (Followers: 3, SJR: 0.367, CiteScore: 1)
Angewandte Schmerztherapie und Palliativmedizin     Hybrid Journal  
Angiogenesis     Hybrid Journal   (Followers: 3, SJR: 2.177, CiteScore: 5)
Animal Cognition     Hybrid Journal   (Followers: 20, SJR: 1.389, CiteScore: 3)
Annales françaises de médecine d'urgence     Hybrid Journal   (Followers: 1, SJR: 0.192, CiteScore: 0)
Annales Henri Poincaré     Hybrid Journal   (Followers: 3, SJR: 1.097, CiteScore: 2)
Annales mathématiques du Québec     Hybrid Journal   (Followers: 4, SJR: 0.438, CiteScore: 0)
Annali dell'Universita di Ferrara     Hybrid Journal   (SJR: 0.429, CiteScore: 0)
Annali di Matematica Pura ed Applicata     Hybrid Journal   (Followers: 1, SJR: 1.197, CiteScore: 1)
Annals of Biomedical Engineering     Hybrid Journal   (Followers: 17, SJR: 1.042, CiteScore: 3)
Annals of Combinatorics     Hybrid Journal   (Followers: 4, SJR: 0.932, CiteScore: 1)
Annals of Data Science     Hybrid Journal   (Followers: 12)
Annals of Dyslexia     Hybrid Journal   (Followers: 10, SJR: 0.85, CiteScore: 2)
Annals of Finance     Hybrid Journal   (Followers: 31, SJR: 0.579, CiteScore: 1)
Annals of Forest Science     Hybrid Journal   (Followers: 7, SJR: 0.986, CiteScore: 2)
Annals of Global Analysis and Geometry     Hybrid Journal   (Followers: 1, SJR: 1.228, CiteScore: 1)
Annals of Hematology     Hybrid Journal   (Followers: 15, SJR: 1.043, CiteScore: 2)
Annals of Mathematics and Artificial Intelligence     Hybrid Journal   (Followers: 12, SJR: 0.413, CiteScore: 1)
Annals of Microbiology     Hybrid Journal   (Followers: 11, SJR: 0.479, CiteScore: 2)
Annals of Nuclear Medicine     Hybrid Journal   (Followers: 4, SJR: 0.687, CiteScore: 2)
Annals of Operations Research     Hybrid Journal   (Followers: 10, SJR: 0.943, CiteScore: 2)
Annals of Ophthalmology     Hybrid Journal   (Followers: 12)
Annals of Regional Science     Hybrid Journal   (Followers: 8, SJR: 0.614, CiteScore: 1)
Annals of Software Engineering     Hybrid Journal   (Followers: 13)
Annals of Solid and Structural Mechanics     Hybrid Journal   (Followers: 9, SJR: 0.239, CiteScore: 1)
Annals of Surgical Oncology     Hybrid Journal   (Followers: 15, SJR: 1.986, CiteScore: 4)
Annals of Telecommunications     Hybrid Journal   (Followers: 9, SJR: 0.223, CiteScore: 1)
Annals of the Institute of Statistical Mathematics     Hybrid Journal   (Followers: 1, SJR: 1.495, CiteScore: 1)
Antonie van Leeuwenhoek     Hybrid Journal   (Followers: 5, SJR: 0.834, CiteScore: 2)
Apidologie     Hybrid Journal   (Followers: 4, SJR: 1.22, CiteScore: 3)
APOPTOSIS     Hybrid Journal   (Followers: 9, SJR: 1.424, CiteScore: 4)
Applicable Algebra in Engineering, Communication and Computing     Hybrid Journal   (Followers: 2, SJR: 0.294, CiteScore: 1)
Applications of Mathematics     Hybrid Journal   (Followers: 2, SJR: 0.602, CiteScore: 1)
Applied Biochemistry and Biotechnology     Hybrid Journal   (Followers: 44, SJR: 0.571, CiteScore: 2)
Applied Biochemistry and Microbiology     Hybrid Journal   (Followers: 18, SJR: 0.21, CiteScore: 1)
Applied Categorical Structures     Hybrid Journal   (Followers: 5, SJR: 0.49, CiteScore: 0)
Applied Composite Materials     Hybrid Journal   (Followers: 49, SJR: 0.58, CiteScore: 2)
Applied Entomology and Zoology     Partially Free   (Followers: 6, SJR: 0.422, CiteScore: 1)
Applied Geomatics     Hybrid Journal   (Followers: 3, SJR: 0.733, CiteScore: 3)
Applied Geophysics     Hybrid Journal   (Followers: 8, SJR: 0.488, CiteScore: 1)
Applied Intelligence     Hybrid Journal   (Followers: 13, SJR: 0.6, CiteScore: 2)
Applied Magnetic Resonance     Hybrid Journal   (Followers: 4, SJR: 0.319, CiteScore: 1)
Applied Mathematics & Optimization     Hybrid Journal   (Followers: 8, SJR: 0.886, CiteScore: 1)
Applied Mathematics - A J. of Chinese Universities     Hybrid Journal   (SJR: 0.17, CiteScore: 0)
Applied Mathematics and Mechanics     Hybrid Journal   (Followers: 5, SJR: 0.461, CiteScore: 1)
Applied Microbiology and Biotechnology     Hybrid Journal   (Followers: 66, SJR: 1.182, CiteScore: 4)
Applied Physics A     Hybrid Journal   (Followers: 10, SJR: 0.481, CiteScore: 2)
Applied Physics B: Lasers and Optics     Hybrid Journal   (Followers: 24, SJR: 0.74, CiteScore: 2)
Applied Psychophysiology and Biofeedback     Hybrid Journal   (Followers: 8, SJR: 0.519, CiteScore: 2)
Applied Research in Quality of Life     Hybrid Journal   (Followers: 12, SJR: 0.316, CiteScore: 1)
Applied Solar Energy     Hybrid Journal   (Followers: 21, SJR: 0.225, CiteScore: 0)
Applied Spatial Analysis and Policy     Hybrid Journal   (Followers: 7, SJR: 0.542, CiteScore: 1)
Aquaculture Intl.     Hybrid Journal   (Followers: 26, SJR: 0.591, CiteScore: 2)
Aquarium Sciences and Conservation     Hybrid Journal   (Followers: 2)
Aquatic Ecology     Hybrid Journal   (Followers: 36, SJR: 0.656, CiteScore: 2)
Aquatic Geochemistry     Hybrid Journal   (Followers: 4, SJR: 0.591, CiteScore: 1)
Aquatic Sciences     Hybrid Journal   (Followers: 13, SJR: 1.109, CiteScore: 3)
Arabian J. for Science and Engineering     Hybrid Journal   (Followers: 5, SJR: 0.303, CiteScore: 1)
Arabian J. of Geosciences     Hybrid Journal   (Followers: 2, SJR: 0.319, CiteScore: 1)
Archaeological and Anthropological Sciences     Hybrid Journal   (Followers: 21, SJR: 1.052, CiteScore: 2)
Archaeologies     Hybrid Journal   (Followers: 12, SJR: 0.224, CiteScore: 0)
Archiv der Mathematik     Hybrid Journal   (Followers: 1, SJR: 0.725, CiteScore: 1)
Archival Science     Hybrid Journal   (Followers: 63, SJR: 0.745, CiteScore: 2)
Archive for History of Exact Sciences     Hybrid Journal   (Followers: 7, SJR: 0.186, CiteScore: 1)
Archive for Mathematical Logic     Hybrid Journal   (Followers: 3, SJR: 0.909, CiteScore: 1)
Archive for Rational Mechanics and Analysis     Hybrid Journal   (SJR: 3.93, CiteScore: 3)
Archive of Applied Mechanics     Hybrid Journal   (Followers: 6, SJR: 0.79, CiteScore: 2)
Archives and Museum Informatics     Hybrid Journal   (Followers: 154, SJR: 0.101, CiteScore: 0)
Archives of Computational Methods in Engineering     Hybrid Journal   (Followers: 6, SJR: 1.41, CiteScore: 5)
Archives of Dermatological Research     Hybrid Journal   (Followers: 7, SJR: 1.006, CiteScore: 2)
Archives of Environmental Contamination and Toxicology     Hybrid Journal   (Followers: 14, SJR: 0.773, CiteScore: 2)
Archives of Gynecology and Obstetrics     Hybrid Journal   (Followers: 17, SJR: 0.956, CiteScore: 2)
Archives of Microbiology     Hybrid Journal   (Followers: 9, SJR: 0.644, CiteScore: 2)
Archives of Orthopaedic and Trauma Surgery     Hybrid Journal   (Followers: 9, SJR: 1.146, CiteScore: 2)
Archives of Osteoporosis     Hybrid Journal   (Followers: 2, SJR: 0.71, CiteScore: 2)
Archives of Sexual Behavior     Hybrid Journal   (Followers: 10, SJR: 1.493, CiteScore: 3)
Archives of Toxicology     Hybrid Journal   (Followers: 17, SJR: 1.541, CiteScore: 5)
Archives of Virology     Hybrid Journal   (Followers: 5, SJR: 0.973, CiteScore: 2)
Archives of Women's Mental Health     Hybrid Journal   (Followers: 15, SJR: 1.274, CiteScore: 3)
Archivio di Ortopedia e Reumatologia     Hybrid Journal  
Archivum Immunologiae et Therapiae Experimentalis     Hybrid Journal   (Followers: 2, SJR: 0.946, CiteScore: 3)
ArgoSpine News & J.     Hybrid Journal  
Argumentation     Hybrid Journal   (Followers: 6, SJR: 0.349, CiteScore: 1)
Arid Ecosystems     Hybrid Journal   (Followers: 2, SJR: 0.2, CiteScore: 0)
Arkiv för Matematik     Hybrid Journal   (Followers: 2, SJR: 0.766, CiteScore: 1)
Arnold Mathematical J.     Hybrid Journal   (Followers: 1, SJR: 0.355, CiteScore: 0)
Arthropod-Plant Interactions     Hybrid Journal   (Followers: 2, SJR: 0.839, CiteScore: 2)
Arthroskopie     Hybrid Journal   (Followers: 1, SJR: 0.131, CiteScore: 0)
Artificial Intelligence and Law     Hybrid Journal   (Followers: 11, SJR: 0.937, CiteScore: 2)
Artificial Intelligence Review     Hybrid Journal   (Followers: 18, SJR: 0.833, CiteScore: 4)
Artificial Life and Robotics     Hybrid Journal   (Followers: 9, SJR: 0.226, CiteScore: 0)
Asia Europe J.     Hybrid Journal   (Followers: 5, SJR: 0.504, CiteScore: 1)
Asia Pacific Education Review     Hybrid Journal   (Followers: 12, SJR: 0.479, CiteScore: 1)
Asia Pacific J. of Management     Hybrid Journal   (Followers: 16, SJR: 1.185, CiteScore: 2)
Asia-Pacific Education Researcher     Hybrid Journal   (Followers: 13, SJR: 0.353, CiteScore: 1)
Asia-Pacific Financial Markets     Hybrid Journal   (Followers: 2, SJR: 0.187, CiteScore: 0)
Asia-Pacific J. of Atmospheric Sciences     Hybrid Journal   (Followers: 19, SJR: 0.855, CiteScore: 1)
Asian Business & Management     Hybrid Journal   (Followers: 9, SJR: 0.378, CiteScore: 1)
Asian J. of Business Ethics     Hybrid Journal   (Followers: 10)
Asian J. of Criminology     Hybrid Journal   (Followers: 6, SJR: 0.543, CiteScore: 1)
AStA Advances in Statistical Analysis     Hybrid Journal   (Followers: 5, SJR: 0.548, CiteScore: 1)
AStA Wirtschafts- und Sozialstatistisches Archiv     Hybrid Journal   (Followers: 5, SJR: 0.183, CiteScore: 0)
ästhetische dermatologie & kosmetologie     Full-text available via subscription  
Astronomy and Astrophysics Review     Hybrid Journal   (Followers: 22, SJR: 3.385, CiteScore: 5)

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Journal Cover
Aquaculture International
Journal Prestige (SJR): 0.591
Citation Impact (citeScore): 2
Number of Followers: 26  
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1573-143X - ISSN (Online) 0967-6120
Published by Springer-Verlag Homepage  [2352 journals]
  • Large-scale production of sea urchin ( Strongylocentrotus intermedius )
           seed in a hatchery in China
    • Authors: John M. Lawrence; Chong Zhao; Ya-Qing Chang
      Pages: 1 - 7
      Abstract: Sea urchins are of major economic importance in aquaculture in China. Strongylocentrotus intermedius, introduced from Japan into Chinese waters, is the major species that is cultured. Seeds are cultured on long-lines or placed into the sea. We provide a description of a seed hatchery in China that produced ca. 18 million seeds of a genetically improved strain of S. intermedius in 2017.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0319-2
      Issue No: Vol. 27, No. 1 (2019)
  • Public attitudes towards marine aquaculture in Canada: insights from the
           Pacific and Atlantic coasts
    • Authors: Mark Flaherty; Gregor Reid; Thierry Chopin; Erin Latham
      Pages: 9 - 32
      Abstract: With 25% of the world’s coastline, Canada is endowed with enormous potential for marine aquaculture. Its development on both the Pacific and Atlantic coasts, however, has come under increasingly intense public scrutiny and generated heated debate over many issues including First Nations territorial rights, impacts on wild fisheries, and environmental impacts. Provincial governments, industry alliances, scientists and ENGOs (environmental non-governmental organizations) have become increasingly embroiled in a communication tug-of-war to win the hearts and minds of the public. To date, however, there has been very limited community-level research into the public’s awareness of aquaculture, the information sources viewed as being most credible, and the issues that resonate the most. This paper reports the results of a study undertaken in small coastal communities on Canada’s Pacific (west) and Atlantic (east) coasts that investigated awareness of aquaculture, and the issues that people associate with its development. While many studies have focused exclusively on attitudes related to either finfish or shellfish farming, this study explores community perceptions related to salmon, shellfish and seaweed farming. Significant differences exist between the Atlantic and Pacific coast respondents in terms of their familiarity with and evaluation of different farming systems, the information sources that they rely upon, and their perceptions of the reliability of different information sources.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0312-9
      Issue No: Vol. 27, No. 1 (2019)
  • Effect of dietary administration of kappa carrageenan extracted from
           Hypnea musciformis on innate immune response, growth, and survival of Nile
           tilapia ( Oreochromis niloticus )
    • Authors: L. Villamil; S. Infante Villamil; G. Rozo; J. Rojas
      Pages: 53 - 62
      Abstract: Immune stimulants are an alternative to antibiotic use and contribute to disease prevention in aquaculture. The effect of dietary administration of kappa carrageenan (Kc), extracted from the red algae Hypnea musciformis, in Nile tilapia (Oreochromis niloticus) was assessed by monitoring weight gain after a 15-day feeding trial. Immunostimulation was inferred by the relative expression of transferrin, interleukin 1β (IL-1β), and growth hormone (GH) in the spleen after 24 h and 15 days of daily administration. The toxic activity of Kc was evaluated in brine shrimp (Artemia salina) nauplii. No significant toxic effects of Kc were observed in A. salina at any dose studied. A positive tendency in growth rate and fish survival values was observed when Kc was administered. Correspondingly, GH, transferrin and IL-1 β levels at day 15 post-treatment were higher in the spleens of fish fed with Kc at 5 g kg−1 relative to non-Kc-treated control fish. Feeding Kc extract from H. musciformis to the fish improved nonspecific immunity parameters and increased survival and growth, but further research, including longer-termed studies, should be conducted before recommendation of Kc supplementation in tilapia diets at commercial scale.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0306-7
      Issue No: Vol. 27, No. 1 (2019)
  • Immunoserology of European seabass ( Dicentrarchus labrax ) and white
           grouper ( Epinephelus aeneus ) as a non-lethal diagnostic tool for viral
           nervous necrosis
    • Authors: Koby Tarrab; Shay Ravid-Peretz; Michal Ucko
      Pages: 63 - 77
      Abstract: Viral nervous necrosis (VNN) is a lethal fish disease that has spread worldwide over the last two decades, causing severe losses in aquaculture. Diagnosis of the infection is generally made by sampling brain tissue, which involves sacrificing often valuable fish. Aiming at developing a non-lethal diagnostic method, the immune responses to an experimental nervous necrosis virus (NNV) infection in sea bass Dicentrarchus labrax and white grouper Epinephelus aeneus, two species most susceptible to the disease, were studied. RT-qPCR revealed presence of NNV in the fish brain within 24 h post-infection, the virus titer remaining high up to 30–35 days post-infection. In D. labrax blood, the virus was detectable within the first 5 days, after which its presence declined rapidly. Mx gene expression correlated to the virus presence in the blood and brain. An indirect ELISA was developed that quantified anti-NNV IgM in the fish blood. In D. labrax, anti-NNV IgM titer increased significantly within 5 days post-infection, and presence of specific IgM was detectable for 180 days. A sandwich ELISA was developed for E. aeneus. In this latter species, anti-NNV IgM titer increased significantly within the first 12 days and was detectable for 208 further days. The sandwich ELISA can be used as a diagnostic tool for detecting NNV exposure in all fish species for which specific antibodies against their IgMs are not yet commercially available. Our immunoserological method can reliably be used for diagnosis of VNN infection and does not require sacrificing the fish.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0307-6
      Issue No: Vol. 27, No. 1 (2019)
  • The effect of dipotassium EDTA and lithium heparin on hematologic values
           of farmed brown trout Salmo trutta (L.) spawners
    • Authors: Mateusz Ciepliński; Mariusz Kasprzak; Monika Grandtke; Aleksandra Steliga; Piotr Kamiński; Leszek Jerzak
      Pages: 79 - 87
      Abstract: Blood analysis is a very important and powerful diagnostic tool in animal health and welfare control. It is routinely performed in higher vertebrates, for which reference values are well established, but fish hematology still needs further research. Many intrinsic and environmental factors have profound impact on fish hematological values, making determination of reference values difficult. Additionally, fish blood usually requires the addition of an anticoagulant agent, because of short clotting times. The choice of anticoagulant is vital for obtaining reliable blood test values. In the present study, the impact of two common anticoagulants, K2EDTA (1.8 mg/ml) and lithium heparin (18 I.U./ml), on hematological values of farmed brown trout Salmo trutta spawners during the spawning season was investigated. Results of basic hematological analysis, such as packed cell volume (PCV), hemoglobin concentration (HGB), red blood cell count (RBC), mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and white blood cell count (WBC), were compared between these two compounds. Statistically significant differences were observed in PCV, MCV, and MCHC, whereas HGB, RBC, MCH, and WBC showed no such differences. These results suggest that lithium heparin gives more reliable results, because red blood cells in K2EDTA-treated samples have a tendency to swell. It is worth noting that ethylenediaminetetraacetic acid salt did not induce sample hemolysis in the present study.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0308-5
      Issue No: Vol. 27, No. 1 (2019)
  • Biodeposits from Mytilus edulis : a potentially high-quality food source
           for the polychaete, Hediste diversicolor
    • Authors: Per Bergström; Niklas Hällmark; Karl-Johan Larsson; Mats Lindegarth
      Pages: 89 - 104
      Abstract: Previous studies have shown clearly that the deposit feeding polychaete, Hediste diversicolor, can promote oxygenation of sediments exposed to excess loads of mussel faeces. In this experimental study, we explicitly test their utility as food for H. diversicolor to survive and grow on. Furthermore, in order to understand the consequences of experimental manipulations, we also evaluated effects on chemical fluxes in and out of the sediment. The results show strong differences in growth but no difference in short-term survival. Fed only on mussel faeces, the polychaetes grew on average 17% in wet weight after a period of 10 days, compared to 3% when given equivalent amounts of organic matter from the natural sediments. Addition of faeces to natural sediments resulted in 19–20% growth, thus suggesting an approximate additive effect of the two food sources. Chemical analyses showed that, oxygen consumption increased with load of organic material irrespective of origin, faecal material caused higher fluxes of ammonia compared to natural organic material, but neither oxygen consumption nor nutrient fluxes were affected by the ashing of sediments. In contrast, fluxes of silicate increased as a consequence of ashing but were not affected by the addition of mussel faeces. Thus, despite risks of experimental artefacts due to ashing of sediments, the results show that oxygen and nutrient dynamics responded to manipulations of organic material and not to the potential modification of sediment structure. Therefore, the observed effects on growth of H. diversicolor can be safely interpreted as caused by differences in amount and quality of organic material. Mussel faeces is a high-quality food source for this species of polychaete and, in combination with ample evidence from previous studies that bioturbation, we conclude that H. diversicolor is a suitable candidate in further efforts to develop technical solutions based on bioturbation for mitigation of adverse effects on benthic environments in connection with mussel-farming.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0309-4
      Issue No: Vol. 27, No. 1 (2019)
  • Environmental stress tolerance and immune response for the small abalone
    • Authors: Weiwei You; Bo Wang; Xuan Luo; Caihuan Ke
      Pages: 105 - 123
      Abstract: Recently, mass mortality affected the cultured small abalone, Haliotis diversicolor diversicolor, which was the dominant cultured abalone species in southern China. Prior studies revealed that survivorship varied significantly between different stocks and crosses. However, the immunological basis for differences in susceptibility has not been well understood to date. Herein, low temperature, air exposure tolerance tests, and pull-off force measurement were assessed in the three groups (Japan, Taiwan, and their Hybrid stock). The results showed that the critical thermal minimum (CTMin) at 50% was 15.6 °C for the Taiwan stock, 12.1 °C for the Japan stock, and 13.2 °C for the Hybrid stock. Upon air exposure challenge, 100% abalones from the Taiwan group died after 8 h at 24 °C, while the survival rate in the Japan and Hybrid groups were 37.8% and 29.4%, respectively. The detachment stress for the Japan group was 42.3 kPa, which was 2.78-fold and 1.43-fold higher compared to the Taiwan and Hybrid groups, respectively. Variation in susceptibility to disease may be based on the effectiveness of the innate immune responses. Therefore, total hemocyte count, phagocytosis, respiratory burst, superoxide dismutase activity, acid phosphatase activity, alkaline phosphatase activity, and myeloperoxidase activity were determined for the healthy abalones in each group. Positive mid-parent heterosis on immunological parameters was consistent with the prior knowledge on the positive mid-parent heterosis for survival rate, which indicated the improvement on immune reaction and disease resistance through hybridization methods. The current study will be useful in efficient design of breeding programs for the development of sustainable abalone aquaculture.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0310-y
      Issue No: Vol. 27, No. 1 (2019)
  • Vibrio harveyi protease deletion mutant as a live attenuated vaccine
           candidate against vibriosis and transcriptome profiling following
           vaccination for Epinephelus fuscoguttatus
    • Authors: Aslizah Mohd-Aris; Mohd-Zamri Saad; Hassan Mohd Daud; Mohd Termizi Yusof; Md Yasin Ina-Salwany
      Pages: 125 - 140
      Abstract: Grouper aquaculture industries have a high risk of being inflicted by bacterial diseases such as vibriosis. Various types of vaccines for vibriosis have been studied throughout the years, yet the potential of live attenuated vaccines remains unsubstantial. Correspondingly, this study attempts to develop a Vibrio harveyi protease deletion mutant into a live attenuated vaccine candidate against vibriosis for Epinephelus fuscoguttatus. Site-directed mutagenesis (SDM) and allelic exchange replacement techniques are employed to synthesize genetically attenuated V. harveyi strain MVh-vhs. The evaluation on safety levels showed that MVh-vhs strain is safe when tested on E. fuscoguttatus. A 100% survival rate with no sign of vibriosis is indicated in fish challenged with the attenuated strain. In contrast, fish challenged with the parental strain showed obvious clinical signs of vibriosis. The median lethal dosage (LD50) of fish challenged with the parental strain is found at 106 CFU/fish. A single dose IP administration of the attenuated strain at 105 CFU/fish following a bacterial challenge at dose 108 CFU/fish is done 4 weeks post vaccination. The vaccinated fish show 52% relative percentage survival (RPS). The transcriptomic profiling following vaccination evoked the regulation of autophagosome pathway and the coagulation and complement cascade pathways as well as antigen processing and presentation pathways. As a conclusion, the V. harveyi attenuated strain MVh-vhs has significant potential to be applied as a live vaccine candidate against vibriosis for E. fuscoguttatus.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0311-x
      Issue No: Vol. 27, No. 1 (2019)
  • Effect of Bacillus subtilis as a probiotic on protein, lipid content, and
           trypsin and chymotrypsin enzymes in rainbow trout biometry ( Oncorhynchus
           mykiss )
    • Authors: Fereshte Sahraei; Hamed Ahari; Shapoor Kakoolaki
      Pages: 141 - 153
      Abstract: This study investigated the effect of Bacillus subtilis IS02 as a probiotic bacterium in four different treatment conditions, namely T1 (no probiotic), T2 (106), T3 (107), and T4 (108) cfu/g of B. subtilis IS02 in the diet of rainbow trout for 30 days, on the protein content, lipid content, trypsin and chymotrypsin enzyme activity, and the bioassay. The protein content and lipid content were measured using the Kjeldahl and Soxhlet methods, respectively.T4 had the highest amount of measured protein (25.30%) while T1 had the lowest amount (15.90). The T4 treatment had a significant effect (P value = 0.001) on improving the protein content as well as the trypsin and chymotrypsin enzyme activity. In contrast, there was no significant difference in terms of the weight gain and specific growth rate between the treatments. Additionally, the results showed that the T3 treatment had a significant effect on increasing the protein content of the carcass, although the effect was not significant in relation to the lipid content. B. subtilis IS02 in a concentration range of 107–108 cfu/g could be employed to improve the protein productive value, trypsin and chymotrypsin enzyme activity, and protein content of the carcass.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0313-8
      Issue No: Vol. 27, No. 1 (2019)
  • A first study on the effect of dietary soya levels and crystalline
           isoflavones on growth, gonad development and gonad histology of farmed
           abalone, Haliotis midae
    • Authors: Yu Wu; Horst Kaiser; Clifford L. W. Jones
      Pages: 167 - 193
      Abstract: Soya in formulated diets for abalone, Haliotis midae, can improve somatic growth. However, it may also influence gonad development, thereby limiting the potential benefit of including soya in feed. This is the first study to determine both the effect of dietary soya level on growth and reproduction of abalone and the role of crystalline isoflavones (ISO) at concentrations occurring in soya diets. Abalone (40–50 g individual−1) were fed seven isonitrogenous and isoenergetic diets. Three diets contained 5, 10 or 19% soya, while an additional three diets included graded levels of ISO. A fishmeal (FM)-only diet was included. The inclusion of ISO did not influence gonad development or abalone growth while soya inclusion level had an effect on both reproductive development and abalone growth. After 180 days, male abalone fed FM with soya inclusion had the highest whole body mass (69.0 ± 2.48 g abalone−1), meat mass index (60.5 ± 1.88%), visceral mass index (13.0 ± 0.27%) and gonad bulk index (42.7 ± 9.82 g abalone−1). Weight loss was observed in all treatments probably due to spawning events. The frequency distribution of the predominant oocyte stage, stage 7 was influenced by soya concentration. The increase in the frequency of stage 7 oocytes in abalone fed FM with soya did not reduce the number of oocytes (45.0 ± 3.01 oocytes mm−2), while the number of oocytes (o) in abalone fed the FM-only diets decreased with increasing abundance of stage 7 oocytes, possibly due to an increase in oocyte size.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0315-6
      Issue No: Vol. 27, No. 1 (2019)
  • A microplate incubation method for assessing egg quality of the barfin
           flounder: effects of well size and rearing medium on larval viability
    • Authors: Tatsuya Unuma; Takashi Ichikawa; Sayumi Sawaguchi; Natsuki Hasegawa
      Pages: 195 - 208
      Abstract: In microplate incubation, fish eggs and larvae are individually reared in small wells. This technique is useful for assessing hatchability, larval survival, and larval abnormality. Nevertheless, the optimal well size (water volume) and rearing media required to ensure full larval viability vary among species. In this study, we examined the effects of well size and rearing medium on hatchability and larval viability of the barfin flounder Verasper moseri and attempted to optimize the incubation conditions for this species. Fertilized eggs were individually stocked in 24-, 48-, and 96-well plates filled with 2, 1, and 0.25 mL seawater containing antibiotics (penicillin G potassium and streptomycin sulfate) and bovine serum albumin (BSA), both of which are required in the rearing media for other species. The eggs were incubated without replacing the medium. Hatch timing and rate were similar in all plate types. Completion of yolk resorption was delayed only in the 96-well plates. In the 24- and 48-well plates, the larvae survived more than 2 weeks after complete yolk resorption until they were starved to death. When the antibiotics were removed from the media, larval mortality increased. In contrast, removal of BSA from the media did not increase yolk sac stage larval mortality for barfin flounder unlike that reported for other species. These results indicate that the use of 24- and 48-well microplates filled with seawater containing antibiotics is optimal for barfin flounder larval viability beyond the completion of yolk resorption.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0316-5
      Issue No: Vol. 27, No. 1 (2019)
  • A review on the impact of seaweed polysaccharide on the growth of
           probiotic bacteria and its application in aquaculture
    • Authors: S. Vidhya Hindu; Natarajan Chandrasekaran; Amitava Mukherjee; John Thomas
      Pages: 227 - 238
      Abstract: The purpose of this review is to elucidate the probiotic and polysaccharide of seaweeds as a dietary supplement for promoting growth and disease resistance in aquatic animals. Because of numerous benefits, the role of dietary supplementation of seaweed polysaccharide and probiotics in promoting growth and interest in commercially important aquatic animals like prawn and fish has generated considerable interest in recent times. Control of bacterial and viral disease and enhancing the immunity of aquatic animals have become urgent needs in aquaculture production. In order to improve the production, several alternative strategies have been raised using probiotics, seaweed polysaccharide (prebiotic), and plant products as biological control agents. Furthermore, the use of natural substances in feed of aquatic animals increases consumer confidence in farmed aquatic animals. Modulation of the intestinal microflora through diet can either be accomplished by means of oral administration of prebiotic compounds or probiotic microorganisms. In some studies for growth enhancement and improvement of immunological parameters, a combination of probiotics and plant products has been reported. The present review suggests the use seaweed polysaccharide and probiotics in combination as dietary supplements due to its beneficial effects to improve the production of healthy aquatic animals.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0318-3
      Issue No: Vol. 27, No. 1 (2019)
  • Effects of autochthonous probiotics, isolated from Tor grypus (Karaman,
           1971) intestine and Lactobacillus casei (PTCC 1608) on expression of
           immune-related genes
    • Authors: Takavar Mohammadian; Mojtaba Alishahi; Mohammad Reza Tabandeh; Abdolhossein Jangaran Nejad; Esmaeil Karami; Mojtaba Zarea
      Pages: 239 - 260
      Abstract: In this study, Lactobacillus plantarum subsp. plantarum and L. delbrukei subsp. bulgaricus were used as autochthonous food supplementation for gene expression and hematological investigation. Then, 480 fish (BW, 45 ± 10 g) were randomly divided into four groups (three replications) and were fed with diet containing 5 × 107 CFU g−1 of each isolated LAB and L. casei (PTCC 1608) and a control diet with no bacteria. Feeding trail was conducted for 60 days, following a change to the non-supplemented feed until 15 days (day 75). Samples from blood and head kidney at days 0, 60, and 75 were taken. The in vitro examination showed that L. plantarum had high antimicrobial activity against the Aeromonas hydrophila and Yesinia ruckeri. Results of this study showed that feeding of T. grypus with L. plantarum supplementation diet led to increase Hb and WBC, and induce TNF-α, IL-8, and IL-1β expressions in the head kidney rather than control. Results suggested that L. plantarum can be applied as a dietary supplement to enhance the immunity system of T. grypus.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0320-9
      Issue No: Vol. 27, No. 1 (2019)
  • Betanodavirus-associated mortality in Asian seabass ( Lates calcarifer ,
           Bloch) cultured in indoor tanks and sea cages
    • Authors: S. R. Krupesha Sharma; M. A. Pradeep; P. N. Dube; T. V. Arun Kumar; Raj Kumar; T. Raja Swaminathan
      Pages: 279 - 286
      Abstract: Sea cage farming of Asian seabass is a prospective aquaculture activity in India. Reports from India on betanodavirus-induced mortality in marine fish culture are not many. An outbreak of viral encephalopathy and retinopathy associated with red-spotted grouper nervous necrosis virus (RGNNV) genotype of betanodavirus in Asian seabass juveniles reared in indoor tanks and sea cages is described in the present paper. Mortality was less severe in cages when compared to tanks. Sequence analysis of the coat protein gene indicated that the virus belonged to RGNNV genotype but found to be distinct from other reported RGNNV isolates. Virus was able to induce cytopathic effects in fish cell lines. Light microscopic features of the disease are also described.
      PubDate: 2019-02-01
      DOI: 10.1007/s10499-018-0322-7
      Issue No: Vol. 27, No. 1 (2019)
  • Heritability estimation of eggs and the newly hatched larvaeof wild
           Persian sturgeon ( Acipenser persicus )
    • Authors: Ali Hallajian; Hossien Ali Abdolhay; Abdol Ahad Shadparvar; Mahtab Yarmohammadi; Mohammad Ali Yazdani Sadati
      Abstract: Persian sturgeon (Acipenser persicus) is one of the most valuable fish species native to the Caspian Sea and Iran’s waters. This study was designed to estimate the heritability of Persian sturgeon eggs and larval hatching. Six fish were caught from the southern Caspian Sea and transferred to “Shahid Beheshti Propagation Complex” during 2016. Cross-breeding was carried out using a 3 × 3 factorial plan. The result of mating among them was nine treatments. For heritability estimates, the total weight, egg size (No. 405), overall weight, and total length of 135 pieces of newly hatched Persian sturgeon larvae were examined. Features including weight and size of larvae and eggs at blastula, gastrula, and neurola stages were evaluated. Weight and length of larvae were measured. Variance components were estimated using software R linear mixed model (LMM) closed Lme 4. Weights at blastula, gastrula, and neurola stages were 0.218 ± 0.124, 0.033 ± 0.01, and 0, respectively. At these stages, heritability of egg size showed 0.375 ± 0.145, 0, and 0, respectively. The heritability of weight and the total length of larvae recorded were 0.231 ± 0.122, 0.161 ± 0.042, respectively. The result of additive genetic value prediction identified three females and three males as the best breeders in the broodstock. The phenotypic correlations between egg weight and size at blastula, gastrula, and neurola stages were 0.106, 0.44, and 0.43, respectively. However, the weight and length correlation of larvae was 0.435. The estimated heritability for these traits supports its suitability as a selection tool in aquaculture.
      PubDate: 2019-02-13
      DOI: 10.1007/s10499-019-00338-w
  • Diversity and bacterial succession of a phototrophic biofilm used as
           complementary food for shrimp raised in a super-intensive culture
    • Authors: Ángel Martín Ortiz-Estrada; Teresa Gollas-Galván; Luis Rafael Martínez-Córdova; Armando Burgos-Hernández; Susana María Scheuren-Acevedo; Mauricio Emerenciano; Marcel Martínez-Porchas
      Abstract: Biofilm-based systems for shrimp aquaculture have emerged as a novel alternative to improve the water quality and obtain a better performance into ponds using zero or limited water exchange. The aims of this work were (1) to evaluate the production response and water quality of a super-intensive shrimp culture using a phototrophic biofilm as complementary food and (2) to study the bacterial diversity of these biofilms. A trial was performed, consisting of a shrimp culture system using biofilms as food source and a control without biofilms. Results showed that the biofilm-based system had better water quality and shrimp registered a higher growth performance compared to the control. Regarding the taxonomic profile of bacterial populations detected in the biofilm, results revealed that Proteobacteria, Bacteroidetes, and Planctomycetes were the three most abundant phyla; of these, eight bacterial orders belonging Rhizobiales, Clostridiales, Cytophagales, Actinomycetales, Vibrionales, Flavobacterales, Planctomycetales, Chlamydiales, and Rhodobacterales constituted a microbial core maintained over time. Some of these are related to anaerobic and aerobic removal processes of nitrogen. The results of this study are a first approach to understand how the bacterial communities forming biofilms are related to water quality of ponds and production performance of aquaculture farms, and how the microbial succession may cause changes on the taxonomic profile biofilms.
      PubDate: 2019-02-06
      DOI: 10.1007/s10499-019-00345-x
  • J. T. Trushenski: Understanding aquaculture
    • Authors: Malcolm Jobling
      PubDate: 2019-02-04
      DOI: 10.1007/s10499-019-00349-7
  • Immune response and resistance of Pacific white shrimp larvae administered
           probiotic, prebiotic, and synbiotic through the bio-encapsulation of
           Artemia sp.
    • Authors: Hamsah Hamsah; Widanarni Widanarni; Alimuddin Alimuddin; Munti Yuhana; Muhammad Zairin Junior; Dendi Hidayatullah
      Abstract: This study aimed to evaluate the immune response and the disease resistance of Pacific white shrimp (Litopenaeus vannamei) larvae administered probiotic Pseudoalteromonas piscicida 1Ub 106 CFU mL−1, prebiotic mannan-oligosaccharide (MOS) 12 mg L−1, and synbiotic (probiotic P. piscicida 1Ub 106 CFU mL−1 and 12 mg L−1 prebiotic MOS) through the bio-encapsulation of Artemia sp. The bio-encapsulation was done in the rearing medium of Artemia sp. for 4 h. The bio-encapsulated Artemia sp. was administered from mysis three to post-larvae (PL) 12. After 13 days of the feeding trial, PL13 were challenged with Vibrio harveyi MR5339 RfR (3 × 107 CFU mL−1) through immersion. The challenge test was performed in the rearing medium (30 individuals L−1) for 5 days. Total hemocyte count (THC), phenoloxidase activity (PO), and respiratory burst activity (RB) were measured before and 3 days after the challenge test, while the shrimp survival was observed until 5 days to evaluate the immune response. The expression of immune-related genes of the shrimp larvae including serine protein, peroxinectin, lipopolysaccharide, and β-1,3-glucan-binding protein was analyzed 24 h after the challenge test. The results demonstrated that THC, PO, RB, the expression of immune-related genes and the survivals of the shrimp larvae administered probiotic, prebiotic, and synbiotic were higher (p < 0.05) than that of control. The administration of probiotic P. piscisida 1Ub, prebiotic MOS, and synbiotic through the bio-encapsulation of Artemia sp. could effectively improve the immune response and the disease resistance of Pacific white shrimp larvae with the best result demonstrated by the synbiotic treatment.
      PubDate: 2019-02-02
      DOI: 10.1007/s10499-019-00346-w
  • Microalgae diet for juveniles of Spondylus limbatu s
    • Authors: A. Marquez; C. Lodeiros; A. Loor; J. Revilla; F. Da Costa; S. Sonnenholzner
      Abstract: The effects of mono- and bi-microalgal diets on shell length, organic tissue, and survival of Spondylus limbatus juveniles were tested in two separate feeding experiments. The first examined nine single species of microalgae: Chaetoceros gracilis, C. muelleri, Isochrysis aff. galbana (T-ISO), Tetraselmis chuii, T. maculata, Pavlova lutheri, Dunaliella salina, Rhodomonas sp., and Nannochloropsis sp. An extra treatment maintaining the juveniles in a long line culture was at Ayangue Bay, Province of Santa Elena, Ecuador. Single diets of P. lutheri, C. muelleri, and C. gracilis showed the best combined growth of survival performance in juveniles, and were therefore selected for the bi-algal diet experiment design with the inclusion of I. galbana T-ISO due to its recommended use in bivalve culture. A second experiment consisted in selecting the best three microalgae species that resulted from the first experiment and combined each other in binary treatments at 1:1 ratio. All bi-algal diets in combination with P. lutheri showed good result in growth (shell and biomass), yield, and survival. However, single P. lutheri mono-algal diet showed equal or better performance index than the other bi-algal combined diets, demonstrating the pertinence in using this microalga as food for S. limbatus juveniles.
      PubDate: 2018-12-17
      DOI: 10.1007/s10499-018-0327-2
  • Bacterial complexes of Bacillus subtilis and Pseudomonas stutzeri alter
           the microbial composition in grass carp water
    • Authors: Xiaoping Zhang; Yang Wang; Zheke Zhong; Qingjun Shao; Yibing Wang; Weifen Li
      Abstract: The current study was designed to evaluate the effects of bacterial complexes (BC, a combination of Bacillus subtilis SC02 and Pseudomonas stutzeri F1M) on nitrogen removal and microbial composition in grass carp culture water. The results of the study showed that the addition of BC could significantly decrease the level of harmful nitrogen compounds and improve the water quality in grass carp culture water over an extended period of time. The 454-pyrosequencing results showed that BC could significantly increase (p < 0.05) the abundance of the genera Hydrogenophaga and Cloacibacterium on the 6th day and 15th day, respectively. LefSe analysis (LDA Score [log10] > 4) indicated that BC enriched in the taxa Firmicutes, Hydrogenophaga, and Pseudomonas, while the CK was enriched in the genus Rhodobacter on the 6th day. On the 15th day, the CK microbiome was characterized by a preponderance of Betaproteobacteria in the water, but no biomarker was identified in the BC group; therefore, the addition of a combination of Bacillus subtilis SC02 and Pseudomonas stutzeri F1M can improve water quality and change the microbiota in grass carp water.
      PubDate: 2018-12-15
      DOI: 10.1007/s10499-018-0325-4
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