Publisher: SciELO   (Total: 911 journals)

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Showing 201 - 400 of 911 Journals sorted alphabetically
Cuadernos de Administración (Universidad del Valle)     Open Access   (Followers: 3)
Cuadernos de Contabilidad     Open Access   (Followers: 3)
Cuadernos de Desarrollo Rural     Open Access   (Followers: 1, SJR: 0.154, CiteScore: 0)
Cuadernos de Economía     Open Access   (Followers: 1, SJR: 0.115, CiteScore: 0)
Cuadernos de Economía     Open Access   (Followers: 3)
Cuadernos de Economia - Latin American J. of Economics     Open Access   (Followers: 4)
Cuadernos de Historia     Open Access   (Followers: 2)
Cuadernos de Historia de la Salud Publica     Open Access  
Cuadernos de Medicina Forense     Open Access   (Followers: 2, SJR: 0.1, CiteScore: 0)
Cuadernos de Psicologia del Deporte     Open Access   (SJR: 0.423, CiteScore: 1)
Cuadernos del CENDES     Open Access   (SJR: 0.109, CiteScore: 0)
Cuadernos.info     Open Access   (SJR: 0.38, CiteScore: 0)
Cubo. A Mathematical J.     Open Access  
Cuestiones Constitucionales     Open Access   (Followers: 2, SJR: 0.1, CiteScore: 0)
Cuicuilco     Open Access   (Followers: 1)
Cuicuilco. Revista de Ciencias Antropológicas     Open Access   (Followers: 1)
Cultivos Tropicales     Open Access   (Followers: 1)
Cultura y Representaciones Sociales     Open Access   (Followers: 1)
Culturales     Open Access   (Followers: 3)
Dados - Revista de Ciências Sociais     Open Access   (SJR: 0.37, CiteScore: 0)
De Jure     Open Access   (Followers: 1)
DELTA : Documentação de Estudos em Lingüística Teórica e Aplicada     Open Access   (SJR: 0.133, CiteScore: 0)
Dementia & Neuropsychologia     Open Access   (Followers: 11, SJR: 0.205, CiteScore: 0)
Dental Press J. of Orthodontics     Open Access   (Followers: 5, SJR: 0.489, CiteScore: 1)
Desacatos     Open Access   (Followers: 1)
Desacatos : Revista de Antropología Social     Open Access   (Followers: 1)
Desarrollo y Sociedad     Open Access   (Followers: 3, SJR: 0.109, CiteScore: 0)
Diálogo Andino - Revista de Historia, Geografía y Cultura Andina     Open Access   (Followers: 1, SJR: 0.116, CiteScore: 0)
Diánoia     Open Access   (Followers: 1)
Dimensión Empresarial     Open Access  
Discusiones Filosóficas     Open Access   (Followers: 1)
Dynamis : Acta Hispanica ad Medicinae Scientiarumque Historiam Illustrandam     Open Access   (Followers: 3, SJR: 0.131, CiteScore: 0)
e-J. of Portuguese History     Open Access   (Followers: 2, SJR: 0.101, CiteScore: 0)
e-Pública : Revista Eletrónica de Direito Público     Open Access   (Followers: 1)
Earth Sciences Research J.     Open Access   (Followers: 1)
Eclética Química     Open Access   (Followers: 1)
Ecología en Bolivia     Open Access  
Economia Aplicada     Open Access   (SJR: 0.208, CiteScore: 0)
Economia e Sociedade     Open Access  
Economía Mexicana. Nueva Época     Open Access   (Followers: 1)
EconoQuantum     Open Access  
Educação & Sociedade     Open Access   (Followers: 3)
Educação & Realidade     Open Access  
Educação e Pesquisa     Open Access   (Followers: 1, SJR: 0.163, CiteScore: 0)
Educação em Revista     Open Access  
Educación Matemática     Open Access  
Educación Médica     Open Access   (Followers: 1, SJR: 0.236, CiteScore: 0)
Educación Médica Superior     Open Access   (Followers: 1, SJR: 0.237, CiteScore: 0)
Educación y Educadores     Open Access  
Educar em Revista     Open Access  
EDUMECENTRO     Open Access  
Eidos     Open Access   (SJR: 0.119, CiteScore: 0)
En-Claves del pensamiento     Open Access   (Followers: 1, SJR: 0.194, CiteScore: 0)
Encuentros     Open Access  
Ene : Revista de Enfermería     Open Access  
Enfermería Global     Open Access   (Followers: 3, SJR: 0.165, CiteScore: 0)
Enfermería Nefrológica     Open Access   (Followers: 1, SJR: 0.198, CiteScore: 0)
Enfoques     Open Access  
Engenharia Agrícola     Open Access   (SJR: 0.305, CiteScore: 1)
Engenharia Sanitaria e Ambiental     Open Access   (SJR: 0.218, CiteScore: 0)
Ensaio Avaliação e Políticas Públicas em Educação     Open Access   (Followers: 1, SJR: 0.254, CiteScore: 0)
Ensayos de Economía     Open Access   (Followers: 1)
Ensayos Revista de Economía     Open Access  
Entomologia y Vectores     Open Access   (Followers: 2)
Entre Ciencia e Ingeniería     Open Access   (Followers: 1)
Epidemiologia e Serviços de Saúde     Open Access  
Escritos de Psicología : Psychological Writings     Open Access   (Followers: 2)
Espacios en Blanco : Revista de educación     Open Access  
Estudios Atacameños     Open Access   (Followers: 2, SJR: 0.446, CiteScore: 1)
Estudios Constitucionales     Open Access   (Followers: 5, SJR: 0.319, CiteScore: 0)
Estudios de Cultura Maya     Open Access   (Followers: 2, SJR: 0.272, CiteScore: 0)
Estudios de Economía     Open Access   (SJR: 0.121, CiteScore: 0)
Estudios de historia moderna y contemporánea de México     Open Access   (SJR: 0.101, CiteScore: 0)
Estudios Economicos     Open Access  
Estudios Filologicos     Open Access   (SJR: 0.107, CiteScore: 0)
Estudios Fronterizos     Open Access   (Followers: 1)
Estudios internacionales     Open Access   (Followers: 5)
Estudios Pedagogicos (Valdivia)     Open Access   (Followers: 1, SJR: 0.338, CiteScore: 0)
Estudios Políticos     Open Access  
Estudios Sociales     Open Access   (Followers: 2)
Estudios sociales : Revista de alimentación contemporánea y desarrollo regional     Open Access   (Followers: 1)
Estudios Socioterritoriales : Revista de Geografía     Open Access  
Estudios y Perspectivas en Turismo     Open Access   (Followers: 2)
Estudos Afro-Asiáticos     Open Access   (Followers: 2)
Estudos Avançados     Open Access   (SJR: 0.232, CiteScore: 0)
Estudos de Psicologia (Campinas)     Open Access   (Followers: 1, SJR: 0.233, CiteScore: 0)
Estudos de Psicologia (Natal)     Open Access   (SJR: 0.117, CiteScore: 0)
Estudos Econômicos     Open Access   (SJR: 0.204, CiteScore: 0)
Estudos Históricos     Open Access   (Followers: 1)
EURE (Santiago) - Revista Latinoamericana de Estudios Urbano Regionales     Open Access   (SJR: 0.38, CiteScore: 1)
Ex aequo     Open Access  
FEM : Revista de la Fundación Educación Médica     Open Access  
Fides et Ratio : Revista de Difusión Cultural y Científica     Open Access   (Followers: 1)
Fisioterapia e Pesquisa     Open Access  
Fisioterapia em Movimento     Open Access  
Fitosanidad     Open Access  
Floresta e Ambiente     Open Access   (Followers: 1, SJR: 0.3, CiteScore: 0)
Folios     Open Access  
Food Science and Technology (Campinas)     Open Access   (Followers: 1, SJR: 0.467, CiteScore: 1)
Formación Universitaria     Open Access   (Followers: 4, SJR: 0.213, CiteScore: 1)
Frónesis     Open Access   (Followers: 1)
Frontera Norte     Open Access  
Gaceta Médica Boliviana     Open Access  
Gaceta Médica Espirituana     Open Access  
Galáxia (São Paulo)     Open Access  
Gayana (Concepcion)     Open Access   (SJR: 0.236, CiteScore: 0)
Gayana. Botanica     Open Access   (SJR: 0.234, CiteScore: 0)
Genetics and Molecular Biology     Open Access   (Followers: 15, SJR: 0.638, CiteScore: 2)
Geofísica internacional     Open Access   (SJR: 0.312, CiteScore: 1)
Gerokomos     Open Access   (Followers: 1, SJR: 0.257, CiteScore: 0)
Gestão & Produção     Open Access   (SJR: 0.156, CiteScore: 0)
Gestión y Política Pública     Open Access   (Followers: 2, SJR: 0.106, CiteScore: 0)
Hacia la Promoción de la Salud     Open Access  
Hallazgos     Open Access   (Followers: 1)
Hidrobiológica     Open Access   (SJR: 0.166, CiteScore: 0)
Historia     Open Access   (Followers: 2)
Historia (Santiago)     Open Access   (Followers: 1, SJR: 0.162, CiteScore: 0)
História (São Paulo)     Open Access   (SJR: 0.11, CiteScore: 0)
História, Ciências, Saúde - Manguinhos     Open Access   (Followers: 2, SJR: 0.144, CiteScore: 0)
Hoehnea     Open Access  
Horizonte Sanitario     Open Access   (Followers: 1)
Horizontes Antropológicos     Open Access   (Followers: 3, SJR: 0.233, CiteScore: 0)
Horticultura Brasileira     Open Access   (SJR: 0.605, CiteScore: 1)
HTS Theological Studies     Open Access   (Followers: 8, SJR: 0.448, CiteScore: 0)
Humanidades Médicas     Open Access  
IDESIA : Revista de Agricultura en Zonas Áridas     Open Access   (SJR: 0.223, CiteScore: 0)
Iheringia. Série Zoologia     Open Access   (Followers: 1, SJR: 0.26, CiteScore: 1)
Íkala : Revista de Lenguaje y Cultura     Open Access   (Followers: 1, SJR: 0.112, CiteScore: 0)
Image & Text     Open Access   (Followers: 1)
Index de Enfermeria     Open Access   (Followers: 7, SJR: 0.113, CiteScore: 0)
Indo-Pacific J. of Phenomenology     Open Access   (Followers: 1)
Infectio     Open Access   (SJR: 0.133, CiteScore: 0)
Información Tecnológica     Open Access   (SJR: 0.197, CiteScore: 0)
Ingeniare : Revista Chilena de Ingenieria     Open Access   (Followers: 1, SJR: 0.239, CiteScore: 0)
Ingeniería Electrónica, Automática y Comunicaciones     Open Access  
Ingeniería Hidráulica y Ambiental     Open Access  
Ingeniería Industrial     Open Access  
Ingeniería Mecánica     Open Access   (Followers: 4)
Ingenieria Mecánica. Tecnologia y Desarrollo     Open Access   (Followers: 3)
Ingeniería y Competitividad     Open Access  
Ingeniería y Desarrollo     Open Access  
Ingenieria y Universidad     Open Access   (Followers: 1, SJR: 0.161, CiteScore: 1)
Ingeniería, Investigación y Tecnología     Open Access   (Followers: 1)
Innovar     Open Access   (SJR: 0.118, CiteScore: 0)
Interações (Campo Grande)     Open Access  
Interface - Comunicação, Saúde, Educação     Open Access   (Followers: 1, SJR: 0.417, CiteScore: 0)
Intersticios Sociales     Open Access   (Followers: 1)
Intervención     Open Access   (Followers: 1)
Intervención Psicosocial     Open Access   (SJR: 0.381, CiteScore: 1)
Intl. Brazilian J. of Urology     Open Access   (Followers: 5, SJR: 0.367, CiteScore: 1)
Intl. J. of Morphology     Open Access  
Intl. J. of Odontostomatology     Open Access   (Followers: 1)
Intl. Law: Revista Colombiana de Derecho Internacional     Open Access   (Followers: 3)
Investigación Administrativa     Open Access   (Followers: 1)
Investigación Bibliotecológica     Open Access   (Followers: 6, SJR: 0.274, CiteScore: 0)
Investigación Económica     Open Access   (Followers: 4, SJR: 0.132, CiteScore: 0)
Investigación y Desarrollo     Open Access   (Followers: 1)
Investigación y Educación en Enfermería     Open Access   (Followers: 1, SJR: 0.198, CiteScore: 0)
Investigaciones Geográficas     Open Access   (SJR: 0.246, CiteScore: 0)
Investigaciones Marinas     Open Access  
Iteckne     Open Access  
Iuris Tantum Revista Boliviana de Derecho     Open Access  
Ius et Praxis     Open Access   (SJR: 0.283, CiteScore: 0)
Izquierdas     Open Access   (SJR: 0.188, CiteScore: 0)
Iztapalapa : Revista de ciencias sociales y humanidades     Open Access   (Followers: 1)
J. of Applied Oral Science     Open Access   (SJR: 0.645, CiteScore: 2)
J. of Applied Research and Technology     Open Access   (SJR: 0.255, CiteScore: 1)
J. of Coloproctology     Open Access   (SJR: 0.167, CiteScore: 0)
J. of Energy in Southern Africa     Open Access   (Followers: 3, SJR: 0.249, CiteScore: 1)
J. of Epilepsy and Clinical Neurophysiology     Open Access   (Followers: 7)
J. of Microwaves, Optoelectronics and Electromagnetic Applications     Open Access   (Followers: 11, SJR: 0.146, CiteScore: 1)
J. of Seed Science     Open Access   (Followers: 1)
J. of Soil Science and Plant Nutrition     Open Access   (Followers: 12, SJR: 0.823, CiteScore: 2)
J. of Technology Management & Innovation     Open Access   (Followers: 5, SJR: 0.271, CiteScore: 1)
J. of the Brazilian Chemical Society     Open Access   (Followers: 2, SJR: 0.357, CiteScore: 1)
J. of the Brazilian Society of Mechanical Sciences     Open Access   (Followers: 2)
J. of the Chilean Chemical Society     Open Access   (Followers: 2, SJR: 0.201, CiteScore: 1)
J. of the Mexican Chemical Society     Open Access   (Followers: 2, SJR: 0.214, CiteScore: 1)
J. of the Selva Andina Research Society     Open Access   (Followers: 3)
J. of the South African Institution of Civil Engineering     Open Access   (Followers: 2, SJR: 0.174, CiteScore: 0)
J. of the Southern African Institute of Mining and Metallurgy     Open Access   (Followers: 6)
J. of Theoretical and Applied Electronic Commerce Research     Open Access   (Followers: 1, SJR: 0.477, CiteScore: 2)
J. of Venomous Animals and Toxins     Open Access   (Followers: 2)
JISTEM : J. of Information Systems and Technology Management     Open Access   (Followers: 7)
Jornal Brasileiro de Nefrologia     Open Access   (SJR: 0.334, CiteScore: 1)
Jornal Brasileiro de Patologia e Medicina Laboratorial     Open Access   (SJR: 0.143, CiteScore: 0)
Jornal Brasileiro de Pneumologia     Open Access   (SJR: 0.448, CiteScore: 1)
Jornal Brasileiro de Psiquiatria     Open Access   (Followers: 3, SJR: 0.224, CiteScore: 0)
Jornal da Sociedade Brasileira de Fonoaudiologia     Open Access  
Jornal de Pneumologia     Open Access  
Jornal Vascular Brasileiro     Open Access   (SJR: 0.136, CiteScore: 0)
Justicia Juris     Open Access  
Kriterion: Revista de Filosofia     Open Access   (Followers: 1, SJR: 0.114, CiteScore: 0)
Kronos : Southern African Histories     Open Access   (Followers: 6)
La ventana. Revista de estudios de género     Open Access  
La zaranda de ideas     Open Access   (Followers: 1)
Laboreal     Open Access  
Lankesteriana     Open Access   (SJR: 0.458, CiteScore: 1)
Latin American Applied Research     Open Access   (Followers: 1)
Latin American J. of Aquatic Research     Open Access   (SJR: 0.289, CiteScore: 1)

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Memórias do Instituto Oswaldo Cruz
Journal Prestige (SJR): 1.172
Citation Impact (citeScore): 2
Number of Followers: 0  

  This is an Open Access Journal Open Access journal
ISSN (Print) 0074-0276 - ISSN (Online) 1678-8060
Published by SciELO Homepage  [911 journals]
  • Rapid detection of Mycobacterium tuberculosis DNA and genetic markers for
           Isoniazid resistance in Ziehl-Neelsen stained slides

    • Abstract: BACKGROUND Early diagnosis of tuberculosis (TB) and identification of strains of Mycobacterium tuberculosis resistant to anti-TB drugs are considered the main factors for disease control. OBJECTIVES To standardise a real-time polymerase chain reaction (qPCR) assay technique and apply it to identify mutations involved in M. tuberculosis resistance to Isoniazid (INH) directly in Ziehl-Neelsen (ZN) stained slides. METHODS Were analysed 55 independent DNA samples extracted from clinical isolates of M. tuberculosis by sequencing. For application in TB diagnosis resistance, 59 ZN-stained slides were used. The sensitivity, specificity and Kappa index, with a 95% confidence interval (CI95%), were determined. FINDINGS The agreement between the tests was, for the katG target, the Kappa index of 0.89 (CI95%: 0.7-1.0). The sensitivity and specificity were 97.6% (CI95%: 87.7-99.9) and 91.7% (CI95%: 61.5-99.5), respectively. For inhA, the Kappa index was 0.92 (CI95%: 0.8-1.0), the sensitivity and specificity were 94.4% (CI95%: 72.7-99.8) and 97.3% (CI95%: 85.8-99.9), respectively. The use of ZN-stained slides for drug-resistant TB detection showed significant results when compared to other standard tests for drug resistance. MAIN CONCLUSIONS qPCR genotyping proved to be an efficient method to detect genes that confer M. tuberculosis resistance to INH. Thus, qPCR genotyping may be an alternative instead of sequencing.
       
  • Leishmania infantum induces high phagocytic capacity and intracellular
           nitric oxide production by human proinflammatory monocyte

    • Abstract: BACKGROUND The mechanism of resistance to SbIII in Leishmania is complex, multifactorial and involves not only biochemical mechanisms, but also other elements, such as the immune system of the host. OBJECTIVES In this study, putative changes in the immunological profile of human monocytes infected with wild-type (WT) and antimony (SbIII)-resistant Leishmania (Viannia) braziliensis and Leishmania (Leishmania) infantum lines were evaluated. METHODS Susceptibility assays WT and SbIII-resistant L. braziliensis and L. infantum were performed using lines THP-1 human monocytic lineage. Phagocytic capacity, cytokine profile, intracellular nitric oxide (NO) production and surface carbohydrate residues profile were performed in peripheral blood monocytes by flow cytometry. FINDINGS The phagocytic capacity and intracellular NO production by classical (CD14++CD16-) and proinflammatory (CD14++CD16+) monocytes were higher in the presence of L. infantum lines compared to L. braziliensis lines. The results also highlight proinflammatory monocytes as the cellular subpopulation of major relevance in a phagocytosis event and NO expression. It is important to note that L. infantum induced a proinflammatory cytokine profile characterised by higher levels of TNF-α in culture supernatant than L. braziliensis. Conversely, both Leishmania lines induce high levels of IL-6 in culture supernatant. Analysis of the expression profile of surface carbohydrates showed that L. braziliensis presents 4.3-fold higher expression of galactose(β1,4)N-acetylglucosamine than L. infantum line. Interestingly, the expression level of α-N-acetylgalactosamine residues was 2-fold lower in the SbIII-resistant L. braziliensis line than its counterpart WT line, indicating differences in surface glycoconjugates between these lines. MAIN CONCLUSIONS Our results showed that L. braziliensis and L. infantum induce different innate immune responses and a highly inflammatory profile, which is characteristic of infection by L. infantum, the species associated with visceral disease.
       
  • DNA nanovaccines prepared using LemA antigen protect Golden Syrian
           hamsters against Leptospira lethal infection

    • Abstract: BACKGROUND Nanoparticles (NPs) are viable candidates as carriers of exogenous materials into cells via transfection and can be used in the DNA vaccination strategy against leptospirosis. OBJECTIVES We evaluated the efficiency of halloysite clay nanotubes (HNTs) and amine-functionalised multi-walled carbon nanotubes (NH2-MWCNTs) in facilitating recombinant LemA antigen (rLemA) expression and protecting Golden Syrian hamsters (Mesocricetus auratus) against Leptospira interrogans lethal infection. METHODS An indirect immunofluorescent technique was used to investigate the potency of HNTs and NH2-MWCNTs in enhancing the transfection and expression efficiency of the DNA vaccine in Chinese hamster ovary (CHO) cells. Hamsters were immunised with two doses of vaccines HNT-pTARGET/lemA, NH2-MWCNTs-pTARGET/lemA, pTARGET/lemA, and empty pTARGET (control), and the efficacy was determined in terms of humoral immune response and protection against a lethal challenge. FINDINGS rLemA DNA vaccines carried by NPs were able to transfect CHO cells effectively, inducing IgG immune response in hamsters (p < 0.05), and did not exhibit cytotoxic effects. Furthermore, 83.3% of the hamsters immunised with NH2-MWCNTs-pTARGET/lemA were protected against the lethal challenge (p < 0.01), and 66.7% of hamsters immunised with HNT-pTARGET/lemA survived (p < 0.05). MAIN CONCLUSIONS NH2-MWCNTs and HNTs can act as antigen carriers for mammalian cells and are suitable for DNA nanovaccine delivery.
       
  • Human papillomavirus type 18 E5 oncoprotein cooperates with E6 and E7 in
           promoting cell viability and invasion and in modulating the cellular redox
           state

    • Abstract: BACKGROUND High-risk human papillomaviruses (HR-HPVs) are the etiological agents of cervical cancer. Among them, types 16 and 18 are the most prevalent worldwide. The HPV genome encodes three oncoproteins (E5, E6, and E7) that possess a high transformation potential in culture cells when transduced simultaneously. In the present study, we analysed how these oncoproteins cooperate to boost key cancer cell features such as uncontrolled cell proliferation, invasion potential, and cellular redox state imbalance. Oxidative stress is known to contribute to the carcinogenic process, as reactive oxygen species (ROS) constitute a potentially harmful by-product of many cellular reactions, and an efficient clearance mechanism is therefore required. Cells infected with HR-HPVs can adapt to oxidative stress conditions by upregulating the formation of endogenous antioxidants such as catalase, glutathione (GSH), and peroxiredoxin (PRX). OBJECTIVES The primary aim of this work was to study how these oncoproteins cooperate to promote the development of certain cancer cell features such as uncontrolled cell proliferation, invasion potential, and oxidative stress that are known to aid in the carcinogenic process. METHODS To perform this study, we generated three different HaCaT cell lines using retroviral transduction that stably expressed combinations of HPV-18 oncogenes that included HaCaT E5-18, HaCaT E6/E7-18, and HaCaT E5/E6/E7-18. FINDINGS Our results revealed a statistically significant increment in cell viability as measured by MTT assay, cell proliferation, and invasion assays in the cell line containing the three viral oncogenes. Additionally, we observed that cells expressing HPV-18 E5/E6/E7 exhibited a decrease in catalase activity and a significant augmentation of GSH and PRX1 levels relative to those of E5, E6/E7, and HaCaT cells. MAIN CONCLUSIONS This study demonstrates for the first time that HPV-18 E5, E6, and E7 oncoproteins can cooperate to enhance malignant transformation.
       
  • Involvement of lipid microdomains in human endothelial cells infected by
           Streptococcus agalactiae type III belonging to the hypervirulent ST-17

    • Abstract: BACKGROUND Streptococcus agalactiae capsular type III strains are a leading cause of invasive neonatal infections. Many pathogens have developed mechanisms to escape from host defense response using the host membrane microdomain machinery. Lipid rafts play an important role in a variety of cellular functions and the benefit provided by interaction with lipid rafts can vary from one pathogen to another. OBJECTIVES This study aims to evaluate the involvement of membrane microdomains during infection of human endothelial cell by S. agalactiae. METHODS The effects of cholesterol depletion and PI3K/AKT signaling pathway activation during S. agalactiae-human umbilical vein endothelial cells (HUVEC) interaction were analysed by pre-treatment with methyl-β-cyclodextrin (MβCD) or LY294002 inhibitors, immunofluorescence and immunoblot analysis. The involvement of lipid rafts was analysed by colocalisation of bacteria with flotillin-1 and caveolin-1 using fluorescence confocal microscopy. FINDINGS In this work, we demonstrated the importance of the integrity of lipid rafts microdomains and activation of PI3K/Akt pathway during invasion of S. agalactiae strain to HUVEC cells. Our results suggest the involvement of flotillin-1 and caveolin-1 during the invasion of S. agalactiae strain in HUVEC cells. CONCLUSIONS The collection of our results suggests that lipid microdomain affects the interaction of S. agalactiae type III belonging to the hypervirulent ST-17 with HUVEC cells through PI3K/Akt signaling pathway.
       
  • Mapping the tuberculosis scientific landscape among BRICS countries: a
           bibliometric and network analysis

    • Abstract: BACKGROUND The five BRICS (Brazil, Russian, Indian, China, and South Africa) countries bear 49% of the world’s tuberculosis (TB) burden and they are committed to ending tuberculosis. OBJECTIVES The aim of this paper is to map the scientific landscape related to TB research in BRICS countries. METHODS Were combined bibliometrics and social network analysis techniques to map the scientific publications related to TB produced by the BRICS. Was made a descriptive statistical data covering the full period of analysis (1993-2016) and the research networks were made for 2007-2016 (8,366 records). The bubble charts were generated by VantagePoint and the networks by the Gephi 0.9.1 software (Gephi Consortium 2010) from co-occurrence matrices produced in VantagePoint. The Fruchterman-Reingold algorithm provided the networks’ layout. FINDINGS During the period 1993-2016, there were 38,315 peer-reviewed, among them, there were 11,018 (28.7%) articles related by one or more authors in a BRICS: India 38.7%; China 23.8%; South Africa 21.1%; Brazil 13.0%; and Russia 4.5% (The total was greater than 100% because our criterion was all papers with at least one author in a BRICS). Among the BRICS, there was greater interaction between India and South Africa and organisations in India and China had the highest productivity; however, South African organisations had more interaction with countries outside the BRICS. Publications by and about BRICS generally covered all research areas, especially those in India and China covered all research areas, although Brazil and South Africa prioritised infectious diseases, microbiology, and the respiratory system. MAIN CONCLUSIONS An overview of BRICS scientific publications and interactions highlighted the necessity to develop a BRICS TB research plan to increase efforts and funding to ensure that basic science research successfully translates into products and policies to help end the TB epidemic.
       
  • Phylogenetics applied to the human immunodeficiency virus type 1 (HIV-1):
           from the cross-species transmissions to the contact network inferences

    • Abstract: Phylogenetic analyses were crucial to elucidate the origin and spread of the pandemic human immunodeficiency virus type 1 (HIV-1) group M virus, both during the pre-epidemic period of cryptic dissemination in human populations as well as during the epidemic phase of spread. The use of phylogenetics and phylodynamics approaches has provided important insights to track the founder events that resulted in the spread of HIV-1 strains across vast geographic areas, specific countries and within geographically restricted communities. In the recent years, the use of phylogenetic analysis combined with the huge availability of HIV sequences has become an increasingly important approach to reconstruct HIV transmission networks and understand transmission dynamics in concentrated and generalised epidemics. Significant efforts to obtain viral sequences from newly HIV-infected individuals could certainly contribute to detect rapidly expanding HIV-1 lineages, identify key populations at high-risk and understand what public health interventions should be prioritised in different scenarios.
       
  • FLI1 gene influences lesion size and skin test may predict therapeutic
           response in cutaneous leishmaniasis

    • Abstract: Genes associated with wound healing have been shown to be risk factors for cutaneous leishmaniasis (CL) which is caused by Leishmania braziliensis. In this study, we examined whether the genes previously associated with CL influenced the clinical outcome. Patients were genotyped and retrospectively classified as responders, who were cured with a single course of pentavalent antimony (Sbv), or as refractories, who did not respond to Sbv. Patients characterised as responders showed a stronger response to the leishmanin skin test (LST) when compared to the refractory subjects (p = 0.0003). Furthermore, we observed an association between the FLI1 CC genotype and an increased size of ulcers (p = 0.0170). We suggest that the leishmanin skin test may be a predictive tool for therapeutic outcome and reinforce FLI1 as a potential influencer of susceptibility and lesion size in CL.
       
  • A new gene inventory of the ubiquitin and ubiquitin-like conjugation
           pathways in Giardia intestinalis

    • Abstract: BACKGROUND Ubiquitin (Ub) and Ub-like proteins (Ub-L) are critical regulators of complex cellular processes such as the cell cycle, DNA repair, transcription, chromatin remodeling, signal translation, and protein degradation. Giardia intestinalis possesses an experimentally proven Ub-conjugation system; however, a limited number of enzymes involved in this process were identified using basic local alignment search tool (BLAST). This is due to the limitations of BLAST’s ability to identify homologous functional regions when similarity between the sequences dips to < 30%. In addition Ub-Ls and their conjugating enzymes have not been fully elucidated in Giardia. OBJETIVE To identify the enzymes involved in the Ub and Ub-Ls conjugation processes using intelligent systems based on the hidden Markov models (HMMs). METHODS We performed an HMM search of functional Pfam domains found in the key enzymes of these pathways in Giardia’s proteome. Each open reading frame identified was analysed by sequence homology, domain architecture, and transcription levels. FINDINGS We identified 118 genes, 106 of which corresponded to the ubiquitination process (Ub, E1, E2, E3, and DUB enzymes). The E3 ligase group was the largest group with 82 members; 71 of which harbored a characteristic RING domain. Four Ub-Ls were identified and the conjugation enzymes for NEDD8 and URM1 were described for first time. The 3D model for Ub-Ls displayed the β-grasp fold typical. Furthermore, our sequence analysis for the corresponding activating enzymes detected the essential motifs required for conjugation. MAIN CONCLUSIONS Our findings highlight the complexity of Giardia’s Ub-conjugation system, which is drastically different from that previously reported, and provides evidence for the presence of NEDDylation and URMylation enzymes in the genome and transcriptome of G. intestinalis.
       
  • Expression of NLRP3 inflammasome in leprosy indicates immune evasion of
           Mycobacterium leprae

    • Abstract: BACKGROUND Leprosy is an infectious-contagious disease caused by Mycobacterium leprae that remain endemic in 105 countries. This neglected disease has a wide range of clinical and histopathological manifestations that are related to the host inflammatory and immune responses. More recently, the inflammasome has assumed a relevant role in the inflammatory response against microbiological agents. However, the involvement of inflammasome in leprosy remains poorly understood. OBJECTIVES The aim is to associate biomarkers of inflammasome with the different immunopathological forms of leprosy. METHODS We performed an observational, cross-sectional, and comparative study of the immunophenotypic expression of inflammasome-associated proteins in immunopathological forms of leprosy of 99 skin lesion samples by immunohistochemistry. The intensity and percentage of NLRP3, Caspase-1, Caspases-4/5, interleukin-1β and interleukin-18 immunoreactivities in the inflammatory infiltrate of skin biopsies were evaluated. FINDINGS Strong expression of NLRP3 and inflammatory Caspases-4/5 were observed in lepromatous leprosy (lepromatous pole). In addition, were observed low expression of caspase-1, interleukin-1β, and interleukin-18 in tuberculoid and lepromatous leprosy. The interpolar or borderline form showed immunophenotype predominantly similar to the lepromatous pole. MAIN CONCLUSIONS Our results demonstrate that the NLRP3 inflammasome is inactive in leprosy, suggesting immune evasion of M. leprae.
       
  • Effects of El Niño-Southern Oscillation on human visceral leishmaniasis
           in the Brazilian State of Mato Grosso do Sul

    • Abstract: BACKGROUND Leishmaniases are considered a major public health problem in South America, specifically in Brazil. Moreover, the transmission and epidemiology of leishmaniasis are possibly associated with climatic and environmental variations. OBJECTIVE This study aimed to assess the association between the extreme climatic phenomenon El Niño-Southern Oscillation (ENSO), the maximum and minimum variations of temperature, precipitation, and soil moisture and the incidence of visceral leishmaniasis (VL) in Mato Grosso do Sul (MS), Brazil, from 2002 to 2015. METHODS The Niño 3.4 index was used for the ENSO variation. The other climatic data were obtained from the climatic tool TerraClimate. Records regarding VL were obtained from the Notification of Injury Information System. FINDINGS From 2002 to 2015, there were 3,137 cases of VL recorded in MS. The annual incidence of the disease was negatively associated with the ENSO index and soil moisture in MS. The VL incidence increased during the negative phase of ENSO and decreased during the positive phase. MAIN CONCLUSIONS The results demonstrated that the interannual cycles of incidence of human VL are significantly associated with the occurrence of the ENSO phenomenon and its phases, El Niño and La Niña.
       
  • Glycogen synthase kinase 3ß participates in late stages of Dengue
           virus-2 infection

    • Abstract: BACKGROUND Viruses can modulate intracellular signalling pathways to complete their infectious cycle. Among these, the PI3K/Akt pathway allows prolonged survival of infected cells that favours viral replication. GSK3β, a protein kinase downstream of PI3K/Akt, gets inactivated upon activation of the PI3K/Akt pathway, and its association with viral infections has been recently established. In this study, the role of GSK3β during Dengue virus-2 (DENV-2) infection was investigated. METHODS GSK3β participation in the DENV-2 replication process was evaluated with pharmacological and genetic inhibition during early [0-12 h post-infection (hpi)], late (12-24 hpi), and 24 hpi in Huh7 and Vero cells. We assessed the viral and cellular processes by calculating the viral titre in the supernatants, In-Cell Western, western blotting and fluorescence microscopy. RESULTS Phosphorylation of GSK3β-Ser9 was observed at the early stages of infection; neither did treatment with small molecule inhibitors nor pre-treatment prior to viral infection of GSK3β reduce viral titres of the supernatant at these time points. However, a decrease in viral titres was observed in cells infected and treated with the inhibitors much later during viral infection. Consistently, the infected cells at this stage displayed plasma membrane damage. Nonetheless, these effects were not elicited with the use of genetic inhibitors of GSK3β. CONCLUSIONS The results suggest that GSK3β participates at the late stages of the DENV replication cycle, where viral activation may promote apoptosis and release of viral particles.
       
  • Oropouche virus detection in saliva and urine

    • Abstract: Oropouche virus (OROV) is an arthropod-borne virus of the Peribunyaviridae family, transmitted to humans primarily by Culicoides paraensis. It is one of the main arboviruses infecting humans in Brazil, primarily in the Amazon Region. Here, we report the detection of OROV in the saliva and urine of a patient whose samples were collected five days after the onset of symptoms. Nucleotide sequencing and phylogenetic analysis further confirmed the results. To our knowledge, this is the first study reporting the detection of OROV in the saliva and urine of an infected patient. In addition, the results of our study expand the current knowledge pertaining to the natural history of Oropouche fever.
       
  • Human acute Chagas disease: changes in factor VII, activated protein C and
           hepatic enzymes from patients of oral outbreaks in Pará State (Brazilian
           Amazon)

    • Abstract: Oral transmission of Chagas disease has been increasing in Latin American countries. The present study aimed to investigate changes in hepatic function, coagulation factor levels and parasite load in human acute Chagas disease (ACD) secondary to oral Trypanosoma cruzi transmission. Clinical and epidemiological findings of 102 infected individuals attended in the State of Pará from October 2013 to February 2016 were included. The most common symptoms were fever (98%), asthenia (83.3%), face and limb edema (80.4%), headache (74.5%) and myalgia (72.5%). The hepatic enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of 30 ACD patients were higher compared with controls, and this increase was independent of the treatment with benznidazole. Moreover, ACD individuals had higher plasma levels of activated protein C and lower levels of factor VII of the coagulation cascade. Patients with the highest parasite load had also the most increased transaminase levels. Also, ALT and AST were associated moderately (r = 0.429) and strongly (r = 0.595) with parasite load respectively. In conclusion, the present study raises the possibility that a disturbance in coagulation and hepatic function may be linked to human ACD.
       
  • Characterisation of the in vitro activity of a
           Nitazoxanide-N-methyl-1H-benzimidazole hybrid molecule against albendazole
           and nitazoxanide susceptible and resistant strains of Giardia intestinalis
           and its in vivo giardicidal activity

    • Abstract: BACKGROUND It was previously demonstrated that CMC-20, a nitazoxanide and N-methyl-1H-benzimidazole hybrid molecule, had higher in vitro activity against Giardia intestinalis WB strain than metronidazole and albendazole and similar to nitazoxanide. OBJETIVES To evaluate the in vitro activity of CMC-20 against G. intestinalis strains with different susceptibility/resistance to albendazole and nitazoxanide and evaluate its effect on the distribution of parasite cytoskeletal proteins and its in vivo giardicidal activity. METHODS CMC-20 activity was tested against two isolates from patients with chronic and acute giardiasis, an experimentally induced albendazole resistant strain and a nitazoxanide resistant clinical isolate. CMC-20 effect on the distribution of parasite cytoskeletal proteins was analysed by indirect immunofluorescence and its activity was evaluated in a murine model of giardiasis. FINDINGS CMC-20 showed broad activity against susceptible and resistant strains to albendazole and nitaxozanide. It affected the parasite microtubule reservoir and triggered the parasite encystation. In this process, alpha-7.2 giardin co-localised with CWP-1 protein. CMC-20 reduced the infection time and cyst load in feces of G. muris infected mice similar to albendazole. MAIN CONCLUSIONS The in vitro and in vivo giardicidal activity of CMC-20 suggests its potential use in the treatment of giardiasis.
       
  • Antiparasitic and anti-inflammatory activities of ß-lapachone-derived
           naphthoimidazoles in experimental acute Trypanosoma cruzi infection

    • Abstract: BACKGROUND Chagas disease, which is caused by the protozoan Trypanosoma cruzi, is endemic to Latin America and mainly affects low-income populations. Chemotherapy is based on two nitrocompounds, but their reduced efficacy encourages the continuous search for alternative drugs. Our group has characterised the trypanocidal effect of naphthoquinones and their derivatives, with naphthoimidazoles derived from β-lapachone (N1, N2 and N3) being the most active in vitro. OBJECTIVES In the present work, the effects of N1, N2 and N3 on acutely infected mice were investigated. METHODS in vivo activity of the compounds was assessed by parasitological, biochemical, histopathological, immunophenotypical, electrocardiographic (ECG) and behavioral analyses. FINDINGS Naphthoimidazoles led to a decrease in parasitaemia (8 dpi) by reducing the number of bloodstream trypomastigotes by 25-50% but not by reducing mortality. N1 protected mice from heart injury (15 dpi) by decreasing inflammation. Bradycardia was also partially reversed after treatment with N1 and N2. Furthermore, the three compounds did not reverse hepatic and renal lesions or promote the improvement of other evaluated parameters. MAIN CONCLUSION N1 showed moderate trypanocidal and promising immunomodulatory activities, and its use in combination with benznidazole and/or anti-arrhythmic drugs as well as the efficacy of its alternative formulations must be investigated in the near future.
       
  • Increasing putative vector importance of Trichophoromyia phlebotomines
           (Diptera: Psychodidae)

    • Abstract: Despite some phlebotomines being well recognised as vectors of leishmaniasis agents, vector importance of those belonging to the genus Trichophoromyia has not been extensively studied. The present study provides evidence regarding the putative vector role played by some species of Trichophoromyia on leishmanine enzootics, based on literature reports and findings obtained from field experiments conducted in the ecotopes of Pará State, Brazil. The species Th. ubiquitalis, Th. velascoi, Th. auraensis, Th. ininii and Th. brachipyga possess minimal criteria to be included in the list of suspected leishmanine vectors. However, knowledge on man-biting behavior, substantiation of vector competence and determination of epidemiological implications are limited for all of the above mentioned species. Published studies together with present data draw attention to prioritize these phlebotomine species in entomological surveillance programs and studies on experimental susceptibility to Leishmania spp. infection.
       
  • Assessment of Culex pipiens bioforms in the world’s
           southernmost distribution limit

    • Abstract: The mosquito Culex pipiens s.s. L. occurs as two bioforms that differ in physiology and behaviour affecting virus transmission cycles. To assess the occurrence of Cx. pipiens bioforms in the southernmost limit of its distribution, specimens were collected aboveground in southern Buenos Aires Province and east Patagonia, Argentina. Ten larvae and 25 adults were individually processed and identified by polymerase chain reaction (PCR) amplification of Ace-2 and CQ11 loci. Culex quinquefasciatus Say (one larva, two adults), Cx. pipiens f. molestus (one larva, one adult) and one adult of hybrid origin were identified in Buenos Aires Province; only Cx. pipiens f. molestus was recorded in Patagonia (eight larvae, 21 adults). The potential absence of bioform pipiens and its implications in arbovirus enzootic cycles is discussed.
       
 
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