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Publisher: SciELO   (Total: 723 journals)

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Showing 1 - 200 of 723 Journals sorted alphabetically
ABCD. Arquivos Brasileiros de Cirurgia Digestiva     Open Access   (Followers: 2, SJR: 0.277, h-index: 5)
ACIMED     Open Access  
Acta Agronómica     Open Access   (Followers: 1, SJR: 0.11, h-index: 2)
Acta Amazonica     Open Access   (Followers: 3, SJR: 0.32, h-index: 18)
Acta Bioethica     Open Access   (Followers: 1, SJR: 0.131, h-index: 4)
Acta Botanica Brasilica     Open Access   (Followers: 2, SJR: 0.364, h-index: 23)
Acta botánica mexicana     Open Access   (Followers: 1, SJR: 0.251, h-index: 6)
Acta Cirurgica Brasileira     Open Access   (SJR: 0.319, h-index: 19)
Acta Limnologica Brasiliensia     Open Access   (Followers: 3, SJR: 0.29, h-index: 6)
Acta Literaria     Open Access   (Followers: 3, SJR: 0.1, h-index: 2)
Acta Medica Colombiana     Open Access   (Followers: 1)
Acta Médica Costarricense     Open Access   (Followers: 2)
Acta Neurológica Colombiana     Open Access   (Followers: 1)
Acta Ortopédica Brasileira     Open Access   (Followers: 1, SJR: 0.288, h-index: 10)
Acta Paulista de Enfermagem     Open Access   (Followers: 2, SJR: 0.242, h-index: 15)
Acta Pediátrica Costarricense     Open Access   (Followers: 1)
Acta Scientiarum. Agronomy     Open Access   (Followers: 4, SJR: 0.961, h-index: 15)
Acta zoológica mexicana     Open Access  
Actualidades Biológicas     Open Access   (Followers: 1)
African Human Rights Law J.     Open Access   (Followers: 19)
African Natural History     Open Access   (Followers: 1, SJR: 0.106, h-index: 4)
Afro-Asia     Open Access  
Ágora - studies in psychoanalytic theory     Open Access   (Followers: 3, SJR: 0.101, h-index: 2)
Agricultura Tecnica     Open Access   (Followers: 6)
Agrociencia     Open Access   (Followers: 1, SJR: 0.213, h-index: 15)
Agrociencia Uruguay     Open Access  
Agronomía Mesoamericana     Open Access  
Aisthesis     Open Access   (Followers: 4, SJR: 0.1, h-index: 1)
Alea : Estudos Neolatinos     Open Access   (Followers: 1, SJR: 0.1, h-index: 3)
Alfa : Revista de Linguística     Open Access  
Alpha (Osorno)     Open Access   (SJR: 0.114, h-index: 3)
Ambiente & sociedade     Open Access   (Followers: 2, SJR: 0.142, h-index: 8)
Ambiente & Agua : An Interdisciplinary J. of Applied Science     Open Access   (Followers: 1, SJR: 0.221, h-index: 4)
Ambiente Construído     Open Access   (Followers: 1)
América Latina en la historia económica     Open Access   (Followers: 2, SJR: 0.148, h-index: 1)
Anais Brasileiros de Dermatologia     Open Access   (Followers: 2, SJR: 0.498, h-index: 23)
Anais da Academia Brasileira de Ciências     Open Access   (Followers: 2, SJR: 0.322, h-index: 42)
Anais do Museu Paulista : História e Cultura Material     Open Access   (Followers: 1)
Anales de Medicina Interna     Open Access   (Followers: 1)
Anales del Instituto de la Patagonia     Open Access   (Followers: 1)
Anales del Sistema Sanitario de Navarra     Open Access   (Followers: 1, SJR: 0.196, h-index: 18)
Análise Psicológica     Open Access   (Followers: 1, SJR: 0.129, h-index: 3)
Análise Social     Open Access   (Followers: 1, SJR: 0.109, h-index: 8)
Andean geology     Open Access   (Followers: 13, SJR: 0.997, h-index: 25)
Annali dell'Istituto Superiore di Sanità     Open Access   (SJR: 0.318, h-index: 29)
Antipoda. Revista de Antropología y Arqueología     Open Access   (Followers: 5, SJR: 0.1, h-index: 0)
Anuario Colombiano de Historia Social y de la Cultura     Open Access   (SJR: 0.101, h-index: 1)
Anuario de Historia Regional y de las Fronteras     Open Access  
Apuntes : Revista de Estudios sobre Patrimonio Cultural - J. of Cultural Heritage Studies     Open Access   (Followers: 3)
Archivos de cardiología de México     Open Access   (Followers: 1, SJR: 0.155, h-index: 13)
Archivos de Medicina Veterinaria     Open Access   (Followers: 2, SJR: 0.199, h-index: 16)
Archivos de Neurociencias     Open Access   (Followers: 3, SJR: 0.1, h-index: 4)
Archivos de Pediatria del Uruguay     Open Access   (Followers: 3)
Archivos de Zootecnia     Open Access   (Followers: 1, SJR: 0.248, h-index: 9)
Archivos Españoles de Urología     Open Access   (SJR: 0.188, h-index: 19)
ARQ     Open Access   (Followers: 4, SJR: 0.1, h-index: 2)
Arquitectura y Urbanismo     Open Access   (Followers: 2)
Arquivo Brasileiro de Medicina Veterinária e Zootecnia     Open Access   (SJR: 0.307, h-index: 22)
Arquivos Brasileiros de Cardiologia     Open Access   (Followers: 1, SJR: 0.334, h-index: 32)
Arquivos Brasileiros de Endocrinologia e Metabologia     Open Access  
Arquivos Brasileiros de Oftalmologia     Open Access   (SJR: 0.308, h-index: 19)
Arquivos de Gastroenterologia     Open Access   (Followers: 1, SJR: 0.424, h-index: 22)
Arquivos de Medicina     Open Access   (SJR: 0.1, h-index: 5)
Arquivos de Neuro-Psiquiatria     Open Access   (SJR: 0.374, h-index: 38)
Arquivos do Instituto Biológico     Open Access   (Followers: 1)
Arquivos Internacionais de Otorrinolaringologia     Open Access  
ARS     Open Access   (Followers: 2)
Atenea (Concepción)     Open Access   (SJR: 0.111, h-index: 3)
Atmósfera     Open Access   (Followers: 2, SJR: 0.377, h-index: 18)
Audiology - Communication Research     Open Access   (Followers: 9)
Avaliação : Revista da Avaliação da Educação Superior (Campinas)     Open Access  
Avances en Odontoestomatologia     Open Access   (Followers: 1, SJR: 0.109, h-index: 4)
Avances en Periodoncia e Implantología Oral     Open Access   (Followers: 1)
Bakhtiniana : Revista de Estudos do Discurso     Open Access  
BAR. Brazilian Administration Review     Open Access   (Followers: 1, SJR: 0.188, h-index: 6)
Biota Neotropica     Open Access   (SJR: 0.373, h-index: 18)
Biotecnología Aplicada     Open Access   (SJR: 0.122, h-index: 10)
Boletim de Ciências Geodésicas     Open Access   (SJR: 0.227, h-index: 5)
Boletim do Museu Paraense Emílio Goeldi. Ciências Humanas     Open Access   (Followers: 1, SJR: 0.139, h-index: 4)
Boletin Chileno de Parasitologia     Open Access  
Boletín de Filología     Open Access  
Boletín de la Sociedad Botánica de México     Open Access  
Boletin de la Sociedad Chilena de Quimica     Open Access  
Boletín de la Sociedad Geológica Mexicana     Open Access   (SJR: 0.231, h-index: 8)
Boletín del Museo Chileno de Arte Precolombino     Open Access   (Followers: 1, SJR: 0.149, h-index: 1)
Bosque     Open Access   (Followers: 2, SJR: 0.256, h-index: 10)
Bragantia     Open Access   (Followers: 2, SJR: 0.522, h-index: 20)
Brazilian Archives of Biology and Technology     Open Access   (Followers: 3, SJR: 0.242, h-index: 31)
Brazilian Dental J.     Open Access   (Followers: 2, SJR: 0.47, h-index: 34)
Brazilian J. of Biology     Open Access   (Followers: 3, SJR: 0.358, h-index: 35)
Brazilian J. of Chemical Engineering     Open Access   (Followers: 3, SJR: 0.424, h-index: 32)
Brazilian J. of Food Technology     Open Access   (Followers: 3)
Brazilian J. of Medical and Biological Research     Open Access   (SJR: 0.541, h-index: 70)
Brazilian J. of Microbiology     Open Access   (Followers: 2, SJR: 0.39, h-index: 38)
Brazilian J. of Oceanography     Open Access   (Followers: 1, SJR: 0.285, h-index: 13)
Brazilian J. of Oral Sciences     Open Access   (Followers: 1, SJR: 0.145, h-index: 6)
Brazilian J. of Physical Therapy     Open Access   (SJR: 0.466, h-index: 16)
Brazilian J. of Plant Physiology     Open Access   (Followers: 3, SJR: 0.452, h-index: 32)
Brazilian J. of Veterinary Research and Animal Science     Open Access   (Followers: 7, SJR: 0.184, h-index: 10)
Brazilian Oral Research     Open Access  
Brazilian Political Science Review     Open Access  
Bulletin of the World Health Organization     Open Access   (Followers: 15, SJR: 2.819, h-index: 123)
Caderno CRH     Open Access   (SJR: 0.102, h-index: 4)
Caderno de Estudos     Open Access  
Cadernos CEDES     Open Access   (Followers: 1, SJR: 0.111, h-index: 5)
Cadernos de Pesquisa     Open Access   (Followers: 2, SJR: 0.26, h-index: 8)
Cadernos de Saúde Pública     Open Access   (Followers: 1, SJR: 0.593, h-index: 55)
Cadernos de Tradução     Open Access  
Cadernos Metrópole     Open Access  
Cadernos Nietzsche     Open Access  
Cadernos Pagu     Open Access   (SJR: 0.179, h-index: 4)
Cadernos Saúde Coletiva     Open Access   (Followers: 1)
Calidad en la educación     Open Access   (Followers: 1)
Cerâmica     Open Access   (Followers: 4, SJR: 0.19, h-index: 11)
CES Medicina     Open Access  
Chilean J. of Agricultural Research     Open Access   (Followers: 1, SJR: 0.366, h-index: 15)
Chungara (Arica) - Revista de Antropologia Chilena     Open Access   (Followers: 1, SJR: 0.49, h-index: 13)
Ciência & Educação (Bauru)     Open Access  
Ciência & Saúde Coletiva     Open Access   (Followers: 2, SJR: 0.588, h-index: 30)
Ciência Animal Brasileira     Open Access   (SJR: 0.322, h-index: 4)
Ciência da Informação     Open Access   (Followers: 1, SJR: 0.117, h-index: 7)
Ciencia del suelo     Open Access   (Followers: 2, SJR: 0.206, h-index: 13)
Ciência e Agrotecnologia     Open Access   (SJR: 0.444, h-index: 19)
Ciencia e Cultura     Open Access   (Followers: 1)
Ciencia e investigación agraria     Open Access   (Followers: 1, SJR: 0.21, h-index: 10)
Ciencia forestal en México     Open Access  
Ciência Rural     Open Access   (Followers: 2, SJR: 0.389, h-index: 24)
Ciencia y Enfermeria - Revista Iberoamericana de Investigacion     Open Access   (Followers: 3, SJR: 0.165, h-index: 7)
Ciencias Marinas     Open Access   (Followers: 2, SJR: 0.348, h-index: 21)
Ciencias Psicológicas     Open Access  
Cirugia Plastica Ibero-Latinoamericana     Open Access   (SJR: 0.175, h-index: 8)
CLEI Electronic J.     Open Access  
Clínica y Salud     Open Access   (SJR: 0.15, h-index: 3)
Clinics     Open Access   (SJR: 0.525, h-index: 36)
CoDAS     Open Access   (SJR: 0.177, h-index: 12)
Computación y Sistemas     Open Access   (SJR: 0.253, h-index: 4)
Comuni@cción     Open Access  
Comunicación y sociedad     Open Access   (Followers: 2, SJR: 0.104, h-index: 1)
Contaduría y Administración     Open Access   (SJR: 0.103, h-index: 1)
Contexto Internacional     Open Access  
Convergencia     Open Access   (SJR: 0.112, h-index: 4)
Correo Científico Médico     Open Access  
Corrosão e Protecção de Materiais     Open Access  
Crop Breeding and Applied Biotechnology     Open Access   (Followers: 4, SJR: 0.604, h-index: 13)
Cuadernos de Economía     Open Access   (Followers: 1)
Cuadernos de Economia - Latin American J. of Economics     Open Access   (Followers: 1)
Cuadernos de Historia     Open Access   (Followers: 3)
Cuadernos de Historia de la Salud Publica     Open Access  
Cuadernos de Medicina Forense     Open Access   (Followers: 2, SJR: 0.106, h-index: 4)
Cuadernos.info     Open Access   (SJR: 0.117, h-index: 2)
Cubo. A Mathematical J.     Open Access  
Cuicuilco     Open Access   (Followers: 1)
Cultivos Tropicales     Open Access  
Culturales     Open Access   (Followers: 2)
Dados - Revista de Ciências Sociais     Open Access   (SJR: 0.429, h-index: 15)
De Jure     Open Access   (Followers: 1)
DELTA : Documentação de Estudos em Lingüística Teórica e Aplicada     Open Access   (SJR: 0.142, h-index: 5)
Dementia & Neuropsychologia     Open Access   (Followers: 4, SJR: 0.232, h-index: 10)
Dental Press J. of Orthodontics     Open Access   (Followers: 2, SJR: 0.214, h-index: 7)
Desacatos     Open Access   (Followers: 1)
Desarrollo y Sociedad     Open Access   (Followers: 3, SJR: 0.106, h-index: 2)
Diálogo Andino - Revista de Historia, Geografía y Cultura Andina     Open Access  
Diánoia     Open Access   (Followers: 1)
Dimensión Empresarial     Open Access  
Dynamis : Acta Hispanica ad Medicinae Scientiarumque Historiam Illustrandam     Open Access   (Followers: 1, SJR: 0.134, h-index: 7)
e-J. of Portuguese History     Open Access   (Followers: 2, SJR: 0.125, h-index: 2)
Eclética Química     Open Access   (Followers: 1)
Ecología en Bolivia     Open Access  
Economia Aplicada     Open Access   (SJR: 0.168, h-index: 6)
Economia e Sociedade     Open Access  
EconoQuantum     Open Access  
Educação & Sociedade     Open Access   (Followers: 3, SJR: 0.244, h-index: 12)
Educação e Pesquisa     Open Access   (Followers: 1, SJR: 0.171, h-index: 8)
Educação em Revista     Open Access  
Educación Matemática     Open Access  
Educación Médica     Open Access   (Followers: 1, SJR: 0.11, h-index: 7)
Educación Médica Superior     Open Access   (Followers: 1, SJR: 0.188, h-index: 7)
Educación y Educadores     Open Access   (Followers: 1)
Educar em Revista     Open Access  
EDUMECENTRO     Open Access  
En-Claves del pensamiento     Open Access   (Followers: 1)
Encuentros     Open Access  
Ene : Revista de Enfermería     Open Access  
Enfermería Global     Open Access   (Followers: 3, SJR: 0.14, h-index: 2)
Enfermería Nefrológica     Open Access   (Followers: 1)
Engenharia Agrícola     Open Access   (SJR: 0.396, h-index: 18)
Engenharia Sanitaria e Ambiental     Open Access   (SJR: 0.15, h-index: 10)
Ensaio Avaliação e Políticas Públicas em Educação     Open Access   (Followers: 1, SJR: 0.19, h-index: 6)
Entomologia y Vectores     Open Access   (Followers: 2)
Escritos de Psicología : Psychological Writings     Open Access   (Followers: 2)
Estudios Atacameños     Open Access   (Followers: 2, SJR: 0.418, h-index: 8)
Estudios Constitucionales     Open Access   (Followers: 4, SJR: 0.383, h-index: 5)
Estudios de Cultura Maya     Open Access   (Followers: 3, SJR: 0.167, h-index: 1)
Estudios de Economía     Open Access   (SJR: 0.144, h-index: 7)
Estudios de historia moderna y contemporánea de México     Open Access   (SJR: 0.101, h-index: 3)
Estudios Filologicos     Open Access   (SJR: 0.105, h-index: 3)
Estudios Fronterizos     Open Access   (Followers: 1)
Estudios internacionales     Open Access   (Followers: 5)
Estudios Pedagogicos (Valdivia)     Open Access   (Followers: 2, SJR: 0.209, h-index: 7)
Estudios Políticos     Open Access  

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Journal Cover Brazilian Journal of Medical and Biological Research
  [SJR: 0.541]   [H-I: 70]   [0 followers]  Follow
    
  This is an Open Access Journal Open Access journal
   ISSN (Print) 0100-879X - ISSN (Online) 1414-431X
   Published by SciELO Homepage  [723 journals]
  • Relationship between matrix metalloproteinases and the occurrence and
           development of ovarian cancer

    • Abstract: Ovarian cancer is one of the most malignant genital cancers, with a high mortality rate. Many researchers have suggested that matrix metalloproteinases (MMPs) have remarkably high expression in ovarian cancer tissues. MMPs are considered to be related to the occurrence, development, invasion and metastasis of ovarian cancer. Moreover, some studies have discovered that the unbalance between MMPs and tissue inhibitor of metalloproteinases (TIMPs) are associated with the malignant phenotype of tumors. This review summarizes the latest research progress of MMPs in ovarian cancer. The investigation of MMP mechanism in ovarian cancer will facilitate the development of effective anti-tumor drugs, and thereby improve the survival rate of patients with ovarian cancer.
       
  • MicroRNA-455 suppresses the oncogenic function of HDAC2 in human
           colorectal cancer

    • Abstract: Colorectal cancer (CRC) is the fourth leading cause of cancer-induced mortality. Histone deacetylase 2 (HDAC2) is involved in prognosis and therapy of CRC. This study aimed to explore novel therapeutic targets for CRC. The alteration of HDAC2 expression in CRC tissues was estimated by qRT-PCR. After lentivirus transfection, HDAC2 knockdown was confirmed by western blot analysis. The effect of HDAC2 knockdown on cell proliferation was then assessed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Screened by TargetScan, microRNA (miR)-455 was predicted to bind to 3′UTR of HDAC2 and the prediction was verified by luciferase assay. Finally, cells were transfected, respectively, with miR-455 mimics or miR-455 negative control (miR-NC) and the expression of HDAC2, cell proliferation and apoptosis of transfected cells were respectively evaluated by western blot analysis, MTT assay and flow cytometry. Results showed that the HDAC2 expression was up-regulated in CRC tissues (P<0.05). HDAC2 knockdown significantly decreased cell viability at day 3 (P<0.05), day 4 (P<0.01), and day 5 (P<0.001) after infection. Then, miR-455 was verified to directly target HDAC2, resulting in a significant difference in luciferase activity (P<0.01). Moreover, miR-455 decreased the expression of HDAC2 (P<0.01). miR-455 remarkably decreased cell viability at day 3 (P<0.05), day 4 (P<0.01), and day 5 (P<0.001) after transfection while inducing cell apoptosis (P<0.001). In conclusion, miR-455 inhibited cell proliferation while inducing cell apoptosis by targeting HDAC2 in CRC cells.
       
  • Characteristics of liver fibrosis with different etiologies using a fully
           quantitative fibrosis assessment tool

    • Abstract: This study aimed to test the diagnostic performance of a fully quantitative fibrosis assessment tool for liver fibrosis in patients with chronic hepatitis B (CHB), primary biliary cirrhosis (PBC) and non-alcoholic steatohepatitis (NASH). A total of 117 patients with liver fibrosis were included in this study, including 50 patients with CHB, 49 patients with PBC and 18 patients with NASH. All patients underwent liver biopsy (LB). Fibrosis stages were assessed by two experienced pathologists. Histopathological images of LB slices were processed by second harmonic generation (SHG)/two-photon excited fluorescence (TPEF) microscopy without staining, a system called qFibrosis (quantitative fibrosis) system. Altogether 101 quantitative features of the SHG/TPEF images were acquired. The parameters of aggregated collagen in portal, septal and fibrillar areas increased significantly with stages of liver fibrosis in PBC and CHB (P<0.05), but the same was not found for parameters of distributed collagen (P>0.05). There was a significant correlation between parameters of aggregated collagen in portal, septal and fibrillar areas and stages of liver fibrosis from CHB and PBC (P<0.05), but no correlation was found between the distributed collagen parameters and the stages of liver fibrosis from those patients (P>0.05). There was no significant correlation between NASH parameters and stages of fibrosis (P>0.05). For CHB and PBC patients, the highest correlation was between septal parameters and fibrosis stages, the second highest was between portal parameters and fibrosis stages and the lowest correlation was between fibrillar parameters and fibrosis stages. The correlation between the septal parameters of the PBC and stages is significantly higher than the parameters of the other two areas (P<0.05). The qFibrosis candidate parameters based on CHB were also applicable for quantitative analysis of liver fibrosis in PBC patients. Different parameters should be selected for liver fibrosis assessment in different stages of PBC compared with CHB.
       
  • Ghrelin plasma levels, gastric ghrelin cell density and bone mineral
           density in women with rheumatoid arthritis

    • Abstract: Generalized bone loss can be considered an extra-articular manifestation of rheumatoid arthritis (RA) that may lead to the occurrence of fractures, resulting in decreased quality of life and increased healthcare costs. The peptide ghrelin has demonstrated to positively affect osteoblasts in vitro and has anti-inflammatory actions, but the studies that correlate ghrelin plasma levels and RA have contradictory results. We aimed to evaluate the correlation between total ghrelin plasma levels, density of ghrelin-immunoreactive cells in the gastric mucosa, and bone mineral density (BMD) in twenty adult women with established RA with 6 months or more of symptoms (mean age of 52.70±11.40 years). Patients with RA presented higher ghrelin-immunoreactive cells density in gastric mucosa (P=0.008) compared with healthy females. There was a positive relationship between femoral neck BMD and gastric ghrelin cell density (P=0.007). However, these same patients presented a negative correlation between plasma ghrelin levels and total femoral BMD (P=0.03). The present results indicate that ghrelin may be involved in bone metabolism of patients with RA. However, the higher density of ghrelin-producing cells in the gastric mucosa of these patients does not seem to induce a corresponding elevation in the plasma levels of this peptide.
       
  • Establishment of patient-derived tumor xenograft (PDTX) models using
           samples from CT-guided percutaneous biopsy

    • Abstract: This study aimed to investigate the feasibility of the establishment of a human cancer xenograft model using samples from computed tomography (CT)-guided percutaneous biopsy. Fresh tumor tissues obtained from 10 cancer patients by CT-guided percutaneous biopsy were subcutaneously inoculated into NOD-Prkdcem26Il2rgem26Nju (NCG) mice to establish human patient-derived tumor xenograft (PDTX) models. The formation of first and second generation xenografts was observed, and tumor volume was recorded over time. Tumor tissue consistency between the PDTX model and primary tumors in patients was compared using H&E staining and immunohistochemistry. Pharmacodynamic tests of clinically used chemotherapeutic drugs were conducted on second generation xenografts, and their effects on tumor growth and body weight were observed. CT-guided percutaneous biopsy samples were successfully collected from 10 patients with advanced cancers. The PDTX model was established in mice using tumor samples obtained from 4 cancer patients, including one small cell carcinoma sample, two adenocarcinoma samples, and one squamous cell carcinoma sample. The success rate was 40%. The obtained PDTX model maintained a degree of differentiation, and morphological and structural characteristics were similar to primary tumors. The pharmacodynamic test of chemotherapeutic drugs in the PDTX model revealed a therapeutic effect on tumor growth, as expected. CT-guided percutaneous biopsy samples can be effectively used to establish a PDTX model, and test these chemotherapy regimens.
       
  • Hippocampal overexpression of Down syndrome cell adhesion molecule in
           amyloid precursor protein transgenic mice

    • Abstract: Down syndrome cell adhesion molecule (DSCAM) is located within the Down syndrome critical region of chromosome 21. DSCAM is a broadly expressed neurodevelopmental protein involved in synaptogenesis, neurite outgrowth, and axon guidance. We previously demonstrated DSCAM overexpression in the cortex of amyloid precursor protein (APP) transgenic mice, suggesting possible regulatory interactions between APP and DSCAM. APP mice exhibit deficits in hippocampus-dependent learning and memory. In this preliminary study, we examined age-related changes in DSCAM expression within the hippocampus in 16 APP transgenic mice (1, 3, 6 and 12 months old). Hippocampus-dependent spatial memory was assessed in APP mice and age-matched wild type littermates (WTs) using the Morris water maze (MWM). The cellular distribution of hippocampal DSCAM and total expression at both mRNA and protein levels were measured by immunohistochemistry, qRT-PCR, and western blotting, respectively. APP mice exhibited spatial memory deficits in the MWM. Intense DSCAM immunoreactivity was observed in the dentate gyrus granule cell layer and hippocampal stratum pyramidale. Total hippocampal DSCAM mRNA and protein expression levels were substantially higher in APP mice than WTs at 1 and 3 months of age. Expression decreased with age in both groups but remained higher in APP mice. DSCAM is overexpressed in the hippocampus over the first 12 months of life in APP mice, but especially during maturation to adulthood. In conclusion, these results suggest an association between DSCAM and APP mice, which is characterized by neuropathology and behavioral deficits. These results provide some clues for future studies on the role of DSCAM overexpression in the precocious cognitive decline observed in APP transgenic mice.
       
  • Etanercept protects rat cardiomyocytes against hypertrophy by regulating
           inflammatory cytokines secretion and cell apoptosis

    • Abstract: We aimed to investigate the effect of etanercept, a tumor necrosis factor-α (TNF-α) inhibitor, on rat cardiomyocyte hypertrophy and its underlying mechanism. Primary neonatal rat cardiomyocytes were isolated from Sprague-Dawley rats. The model of rat cardiomyocyte hypertrophy was induced by endothelin, and then treated with different concentrations of etanercept (1, 10, and 50 μM). After treatment, cell counts, viability and cell apoptosis were evaluated. The mRNA levels of myocardial hypertrophy marker genes, including atrial natriuretic factor (ANF), matrix metalloproteinase (MMP)-9 and MMP-13, were detected by qRT-PCR, and the expressions of apoptosis-related proteins (Bcl-2 and Bax) were measured by western blotting. The protein levels of transforming growth factor-β1 (TGF-β1), interleukin (IL)-1β, IL-6, leukemia inhibitory factor (LIF) and cardiotrophin-1 (CT-1) were determined using enzyme linked immunosorbent assay (ELISA) kits. In the present study, TNF-α level in cardiomyocytes with hypertrophy was significantly enhanced (P<0.05). Compared to the model group, cell number and viability were significantly increased and ratio of apoptotic cells was reduced by etanercept (P<0.05, P<0.01, or P<0.001). In addition, etanercept remarkably reduced the mRNA levels of ANF, MMP-9 and MMP-13, inhibited the expression of Bax, and increased the expression of Bcl-2 compared to the model group (P<0.05). ELISA results further showed that etanercept lowered the levels of IL-1β, IL-6, LIF and CT-1 but not TGF-β1 compared to the model group (P<0.05). Etanercept may protect rat cardiomyocytes from hypertrophy by inhibiting inflammatory cytokines secretion and cell apoptosis.
       
  • Association between MFN2 gene polymorphisms and the risk and prognosis of
           acute liver failure: a case-control study in a Chinese population

    • Abstract: This study aimed to determine the role of mitofusin 2 (MFN2) gene polymorphisms in the risk and prognosis of acute liver failure (ALF). A total of 298 blood samples were collected from 138 ALF patients (case group) and 160 healthy participants (control group). Coagulation function, glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), total bilirubin (TB), blood ammonia and lactic acid (LA) were measured. The predictive evaluation of MFN2 gene polymorphisms in the risk and prognosis of ALF patients was estimated using Kaplan-Meier survival analysis, haplotype analysis, binary logistic regression analysis and Cox regression analysis. Higher levels of GPT, GOT, TB, blood ammonia and LA were observed in ALF patients with the GG genotype of rs873457 or the TT genotype of rs4846085 than in those with the CC genotype of these two SNPs. The GTACAGC and GTGTGGC haplotypes were a protective factor and a risk factor for ALF, respectively. Blood ammonia and LA levels were independent risk factors and the CC genotype of rs873457 and the CC genotype of rs4846085 were protective factors for ALF. ALF patients with the GG genotype of rs873457 or the TT genotype of rs4846085 had a lower survival rate than those with other genotypes of these two SNPs. The rs4846085 and rs873457 polymorphisms were both independent factors affecting the prognosis of ALF patients. MFN2 gene polymorphisms (rs873457, rs2336384, rs1474868, rs4846085 and rs2236055) may be associated with ALF and the rs873457 and rs4846085 polymorphisms are correlated with the risk and prognosis of ALF.
       
  • Adiponectin inhibits oxidization-induced differentiation of T helper cells
           through inhibiting costimulatory CD40 and CD80

    • Abstract: Adiponectin is a multifunctional adipokine that has several oligomeric forms in the blood stream, which broadly regulates innate and acquired immunity. Therefore, in this study, we aimed to observe the differentiation of T helper (Th) cells and expression of costimulatory signaling molecules affected by adiponectin. The mRNA and protein expression levels of adiponectin and its receptors in oxidized low density lipoprotein cholesterol-treated endothelial cells were assayed by real time PCR and immunofluorescence. The endothelial cells were then treated with adiponectin with or without adipoR1 or adipoR2 siRNA and co-cultured with T lymphocytes. The distribution of Th1, Th2 and Th17 subsets were assayed by flow cytometry. The effects of adiponectin on costimulatory signaling molecules HLA-DR, CD80, CD86 and CD 40 was also assayed by flow cytometry. The results showed that endothelial cells expressed adiponectin and its receptor adipoR1 and adipoR2, but not T-cadherin. Adiponectin suppressed Th1 and Th17 differentiation through adipoR1 receptor, contributed to the inhibition of CD80 and CD40, and inhibited differentiation of Th1 and Th17 by inhibiting antigen presenting action.
       
  • Attenuation of renal ischemic reperfusion injury by salvianolic acid B via
           suppressing oxidative stress and inflammation through PI3K/Akt signaling
           pathway

    • Abstract: Salvianolic acid B (SAB) is one the major phytocomponents of Radix Salvia miltiorrhiza and exhibit numerous health promoting properties. The objective of the current study was to examine whether SAB exerts a renoprotective effect by attenuating oxidative stress and inflammatory response through activating phosphatidylinositol 3-kinase/serine-threonine kinase B (PI3K/Akt) signaling pathway in a renal ischemic reperfusion rat model. Forty Sprague-Dawley male rats (250–300 g) were obtained and split into four groups with ten rats in each group. The right kidney of all rats was removed (nephrectomy). The rats of the Control group received only saline (occlusion) and served as a sham control group, whereas rats subjected to ischemic reperfusion (IR) insult by clamping the left renal artery served as a postitive control group. The other 2 groups of rats were pretreated with SAB (20 and 40 mg·kg-1·day-1) for 7 days prior IR induction and served as treatment groups (SAB 20+IR; SAB 40+IR). Renal markers creatinine (Cr) and blood urea nitrogen (BUN) were significantly lower in the groups that received SAB. Pretreatment with SAB appears to attenuate oxidative stress by suppressing the production of lipid peroxidation products like malondialdehyde as well as elevating antioxidant activity. The concentration of inflammatory markers and neutrophil infiltration (myeloperoxidase) were significantly decreased. Meanwhile, PI3K protein expression and pAkt/Akt ratio were significantly upregulated upon supplementation with SAB, indicating its renoprotective activity. Taken together, these results indicate that SAB can therapeutically alleviate oxidative stress and inflammatory process via modulating PI3K/Akt signaling pathway and probably ameliorate renal function and thus act as a renoprotective agent.
       
  • Species distribution and antifungal susceptibility patterns of Candida
           isolates from a public tertiary teaching hospital in the Eastern Cape
           Province, South Africa

    • Abstract: Candida species are the leading cause of invasive fungal infections, and over the past decade there has been an increased isolation of drug resistant Candida species. This study aimed to identify the species distribution of Candida isolates and to determine their unique antifungal susceptibility and resistance patterns. During a cross-sectional study, 209 Candida isolates (recovered from 206 clinical samples) were collected and their species distribution was determined using ChromAgar Candida. The Vitek-2 system (Biomerieux, South Africa) was used to determine minimum inhibitory concentrations (MICs) to azoles (fluconazole, voriconazole), echinocandins (caspofungin, micafungin), polyenes (amphotericin B) and flucytosine. Four species of Candida were isolated, of which C. albicans was the most frequent, isolated in 45.4% (95/209) of the isolates, followed by C. glabrata: 31.1% (65/209). The MICs of the different antifungal drugs varied amongst the species of Candida. From the 130 isolates tested for MICs, 90.77% (112/130) were susceptible to all antifungal drugs and 6.9% (9/130) of the isolates were multi-drug resistant. C. dubliniensis (n=2) isolates were susceptible to all the above mentioned antifungal drugs. There was no significant difference in species distribution amongst clinical specimens and between patients' genders (P>0.05). An increase in MIC values for fluconazole and flucytosine towards the resistance range was observed. To our knowledge, this is the first report on surveillance of Candida species distribution and antifungal susceptibility at a public tertiary teaching hospital in Eastern Cape, South Africa.
       
  • Serum microRNA-30c levels are correlated with disease progression in
           Xinjiang Uygur patients with chronic hepatitis B

    • Abstract: We aimed to investigate the potential role and mechanism of microRNA-30c (miR-30c) in the pathological development of chronic hepatitis B (CHB). The serum levels of miR-30c in hepatitis B virus (HBV) carrier Xinjiang Uygur patients with inactive, low-replicative, high-replicative and HBe antigen-positive CHB were investigated. HepG2 cells were co-transfected with pHBV1.3 and miR-30c mimic or inhibitor or scramble RNA. The effects of miR-30c dysregulation on HBV replication and gene expression, cell proliferation and cell cycle were then investigated. miR-30c was down-regulated in Xinjiang Uygur patients with CHB compared to healthy controls and its expression level discriminated HBV carrier patients with inactive, low-replicative, high-replicative and HBe antigen-positive risk for disease progression. Overexpression of miR-30c significantly inhibited HBV replication and the expressions of HBV pgRNA, capsid-associated virus DNA and Hbx in hepatoma cells. Moreover, overexpression of miR-30c significantly inhibited cell proliferation and delayed G1/S phase transition in hepatoma cells. Opposite effects were obtained after suppression of miR-30c. Our results indicate that miR-30c was down-regulated in Xinjiang Uygur patients with CHB, and miR-30c levels could serve as a marker for risk stratification of HBV infection. Down-regulation of miR-30c may result in the progression of CHB via promoting HBV replication and cell proliferation.
       
  • Rimonabant improves metabolic parameters partially attributed to
           restoration of high voltage-activated Ca2+ channels in skeletal muscle in
           HFD-fed mice

    • Abstract: Cannabinoid type 1 receptor (CB1R) inhibition tends to be one of the promising strategies for the treatment of obesity and other related metabolic disorders. Although CB1R inhibition may cause adverse psychiatric effects including depression and anxiety, the investigation of the role of peripheral CB1R on weight loss and related metabolic parameters are urgently needed. We first explored the effect of rimonabant, a selective CB1R antagonist/inverse agonist, on some metabolic parameters in high fat-diet (HFD)-induced obesity in mice. Then, real-time PCR and electrophysiology were used to explore the contribution of high voltage-activated Ca2+ channels (HVACCs), especially Cav1.1, on rimonabant's effect in skeletal muscle (SM) in HFD-induced obesity. Five-week HFD feeding caused body weight gain, and decreased glucose/insulin tolerance in mice compared to those in the regular diet group (P<0.05), which was restored by rimonabant treatment compared to the HFD group (P<0.05). Interestingly, HVACCs and Cav1.1 were decreased in soleus muscle cells in the HFD group compared to the control group. Daily treatment with rimonabant for 5 weeks was shown to counter such decrease (P<0.05). Collectively, our findings provided a novel understanding for peripheral CB1R's role in the modulation of body weight and glucose homeostasis and highlight peripheral CB1R as well as Cav1.1 in the SM as potential targets for obesity treatment.
       
  • FAMLF is a target of miR-181b in Burkitt lymphoma

    • Abstract: Burkitt lymphoma (BL) is a highly malignant non-Hodgkin's lymphoma that is closely related to the abnormal expression of genes. Familial acute myelogenous leukemia related factor (FAMLF; GenBank accession No. EF413001.1) is a novel gene that was cloned by our research group, and miR-181b is located in the intron of the FAMLF gene. To verify the role of miR-181b and FAMLF in BL, RNAhybrid software was used to predict target site of miR-181b on FAMLF and real-time quantitative PCR (RQ-PCR) was used to detect expression of miR-181b and FAMLF in BL patients, Raji cells and unaffected individuals. miR-181b was then transfected into Raji and CA46 cell lines and FAMLF expression was examined by RQ-PCR and western blotting. Further, Raji cells viability and proliferation were detected by MTT and clone formation, and Raji cell cycle and apoptosis were detected by flow cytometry. The results showed that miR-181b can bind to bases 21–42 of the FAMLF 5′ untranslated region (UTR), FAMLF was highly expressed and miR-181b was lowly expressed in BL patients compared with unaffected individuals. FAMLF expression was significantly and inversely correlated to miR-181b expression, and miR-181b negatively regulated FAMLF at posttranscriptional and translational levels. A dual-luciferase reporter gene assay identified that the 5′ UTR of FAMLF mRNA contained putative binding sites for miR-181b. Down-regulation of FAMLF by miR-181b arrested cell cycle, inhibited cell viability and proliferation in a BL cell line model. Our findings explain a new mechanism of BL pathogenesis and may also have implications in the therapy of FAMLF-overexpressing BL.
       
 
 
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