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Publisher: John Wiley and Sons   (Total: 1583 journals)

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Showing 1 - 200 of 1583 Journals sorted alphabetically
Abacus     Hybrid Journal   (Followers: 12, SJR: 0.48, h-index: 22)
About Campus     Hybrid Journal   (Followers: 5)
Academic Emergency Medicine     Hybrid Journal   (Followers: 55, SJR: 1.385, h-index: 91)
Accounting & Finance     Hybrid Journal   (Followers: 44, SJR: 0.547, h-index: 30)
ACEP NOW     Free  
Acta Anaesthesiologica Scandinavica     Hybrid Journal   (Followers: 50, SJR: 1.02, h-index: 88)
Acta Archaeologica     Hybrid Journal   (Followers: 143, SJR: 0.101, h-index: 9)
Acta Geologica Sinica (English Edition)     Hybrid Journal   (Followers: 3, SJR: 0.552, h-index: 41)
Acta Neurologica Scandinavica     Hybrid Journal   (Followers: 5, SJR: 1.203, h-index: 74)
Acta Obstetricia et Gynecologica Scandinavica     Hybrid Journal   (Followers: 15, SJR: 1.197, h-index: 81)
Acta Ophthalmologica     Hybrid Journal   (Followers: 5, SJR: 0.112, h-index: 1)
Acta Paediatrica     Hybrid Journal   (Followers: 54, SJR: 0.794, h-index: 88)
Acta Physiologica     Hybrid Journal   (Followers: 7, SJR: 1.69, h-index: 88)
Acta Polymerica     Hybrid Journal   (Followers: 9)
Acta Psychiatrica Scandinavica     Hybrid Journal   (Followers: 35, SJR: 2.518, h-index: 113)
Acta Zoologica     Hybrid Journal   (Followers: 5, SJR: 0.459, h-index: 29)
Acute Medicine & Surgery     Hybrid Journal   (Followers: 2)
Addiction     Hybrid Journal   (Followers: 32, SJR: 2.086, h-index: 143)
Addiction Biology     Hybrid Journal   (Followers: 11, SJR: 2.091, h-index: 57)
Adultspan J.     Hybrid Journal   (SJR: 0.127, h-index: 4)
Advanced Energy Materials     Hybrid Journal   (Followers: 24, SJR: 6.411, h-index: 86)
Advanced Engineering Materials     Hybrid Journal   (Followers: 25, SJR: 0.81, h-index: 81)
Advanced Functional Materials     Hybrid Journal   (Followers: 49, SJR: 5.21, h-index: 203)
Advanced Healthcare Materials     Hybrid Journal   (Followers: 13, SJR: 0.232, h-index: 7)
Advanced Materials     Hybrid Journal   (Followers: 253, SJR: 9.021, h-index: 345)
Advanced Materials Interfaces     Hybrid Journal   (Followers: 6, SJR: 1.177, h-index: 10)
Advanced Optical Materials     Hybrid Journal   (Followers: 5, SJR: 2.488, h-index: 21)
Advanced Science     Open Access   (Followers: 4)
Advanced Synthesis & Catalysis     Hybrid Journal   (Followers: 17, SJR: 2.729, h-index: 121)
Advances in Polymer Technology     Hybrid Journal   (Followers: 13, SJR: 0.344, h-index: 31)
Africa Confidential     Hybrid Journal   (Followers: 19)
Africa Research Bulletin: Economic, Financial and Technical Series     Hybrid Journal   (Followers: 12)
Africa Research Bulletin: Political, Social and Cultural Series     Hybrid Journal   (Followers: 9)
African Development Review     Hybrid Journal   (Followers: 33, SJR: 0.275, h-index: 17)
African J. of Ecology     Hybrid Journal   (Followers: 14, SJR: 0.477, h-index: 39)
Aggressive Behavior     Hybrid Journal   (Followers: 15, SJR: 1.391, h-index: 66)
Aging Cell     Open Access   (Followers: 9, SJR: 4.374, h-index: 95)
Agribusiness : an Intl. J.     Hybrid Journal   (Followers: 6, SJR: 0.627, h-index: 14)
Agricultural and Forest Entomology     Hybrid Journal   (Followers: 14, SJR: 0.925, h-index: 43)
Agricultural Economics     Hybrid Journal   (Followers: 44, SJR: 1.099, h-index: 51)
AIChE J.     Hybrid Journal   (Followers: 29, SJR: 1.122, h-index: 120)
Alcoholism and Drug Abuse Weekly     Hybrid Journal   (Followers: 7)
Alcoholism Clinical and Experimental Research     Hybrid Journal   (Followers: 7, SJR: 1.416, h-index: 125)
Alimentary Pharmacology & Therapeutics     Hybrid Journal   (Followers: 34, SJR: 2.833, h-index: 138)
Alimentary Pharmacology & Therapeutics Symposium Series     Hybrid Journal   (Followers: 3)
Allergy     Hybrid Journal   (Followers: 49, SJR: 3.048, h-index: 129)
Alternatives to the High Cost of Litigation     Hybrid Journal   (Followers: 3)
American Anthropologist     Hybrid Journal   (Followers: 129, SJR: 0.951, h-index: 61)
American Business Law J.     Hybrid Journal   (Followers: 24, SJR: 0.205, h-index: 17)
American Ethnologist     Hybrid Journal   (Followers: 90, SJR: 2.325, h-index: 51)
American J. of Economics and Sociology     Hybrid Journal   (Followers: 28, SJR: 0.211, h-index: 26)
American J. of Hematology     Hybrid Journal   (Followers: 31, SJR: 1.761, h-index: 77)
American J. of Human Biology     Hybrid Journal   (Followers: 12, SJR: 1.018, h-index: 58)
American J. of Industrial Medicine     Hybrid Journal   (Followers: 16, SJR: 0.993, h-index: 85)
American J. of Medical Genetics Part A     Hybrid Journal   (Followers: 15, SJR: 1.115, h-index: 61)
American J. of Medical Genetics Part B: Neuropsychiatric Genetics     Hybrid Journal   (Followers: 3, SJR: 1.771, h-index: 107)
American J. of Medical Genetics Part C: Seminars in Medical Genetics     Partially Free   (Followers: 5, SJR: 2.315, h-index: 79)
American J. of Orthopsychiatry     Hybrid Journal   (Followers: 4, SJR: 0.756, h-index: 69)
American J. of Physical Anthropology     Hybrid Journal   (Followers: 36, SJR: 1.41, h-index: 88)
American J. of Political Science     Hybrid Journal   (Followers: 250, SJR: 5.101, h-index: 114)
American J. of Primatology     Hybrid Journal   (Followers: 15, SJR: 1.197, h-index: 63)
American J. of Reproductive Immunology     Hybrid Journal   (Followers: 3, SJR: 1.347, h-index: 75)
American J. of Transplantation     Hybrid Journal   (Followers: 15, SJR: 2.792, h-index: 140)
American J. on Addictions     Hybrid Journal   (Followers: 9, SJR: 0.843, h-index: 57)
Anaesthesia     Hybrid Journal   (Followers: 118, SJR: 1.404, h-index: 88)
Analyses of Social Issues and Public Policy     Hybrid Journal   (Followers: 11, SJR: 0.397, h-index: 18)
Analytic Philosophy     Hybrid Journal   (Followers: 16)
Anatomia, Histologia, Embryologia: J. of Veterinary Medicine Series C     Hybrid Journal   (Followers: 3, SJR: 0.295, h-index: 27)
Anatomical Sciences Education     Hybrid Journal   (Followers: 1, SJR: 0.633, h-index: 24)
Andrologia     Hybrid Journal   (Followers: 2, SJR: 0.528, h-index: 45)
Andrology     Hybrid Journal   (Followers: 2, SJR: 0.979, h-index: 14)
Angewandte Chemie     Hybrid Journal   (Followers: 157)
Angewandte Chemie Intl. Edition     Hybrid Journal   (Followers: 212, SJR: 6.229, h-index: 397)
Animal Conservation     Hybrid Journal   (Followers: 34, SJR: 1.576, h-index: 62)
Animal Genetics     Hybrid Journal   (Followers: 8, SJR: 0.957, h-index: 67)
Animal Science J.     Hybrid Journal   (Followers: 6, SJR: 0.569, h-index: 24)
Annalen der Physik     Hybrid Journal   (Followers: 5, SJR: 1.46, h-index: 40)
Annals of Anthropological Practice     Partially Free   (Followers: 2, SJR: 0.187, h-index: 5)
Annals of Applied Biology     Hybrid Journal   (Followers: 8, SJR: 0.816, h-index: 56)
Annals of Clinical and Translational Neurology     Open Access   (Followers: 1)
Annals of Human Genetics     Hybrid Journal   (Followers: 9, SJR: 1.191, h-index: 67)
Annals of Neurology     Hybrid Journal   (Followers: 43, SJR: 5.584, h-index: 241)
Annals of Noninvasive Electrocardiology     Hybrid Journal   (Followers: 2, SJR: 0.531, h-index: 38)
Annals of Public and Cooperative Economics     Hybrid Journal   (Followers: 9, SJR: 0.336, h-index: 23)
Annals of the New York Academy of Sciences     Hybrid Journal   (Followers: 5, SJR: 2.389, h-index: 189)
Annual Bulletin of Historical Literature     Hybrid Journal   (Followers: 12)
Annual Review of Information Science and Technology     Hybrid Journal   (Followers: 14)
Anthropology & Education Quarterly     Hybrid Journal   (Followers: 24, SJR: 0.72, h-index: 31)
Anthropology & Humanism     Hybrid Journal   (Followers: 16, SJR: 0.137, h-index: 3)
Anthropology News     Hybrid Journal   (Followers: 14)
Anthropology of Consciousness     Hybrid Journal   (Followers: 11, SJR: 0.172, h-index: 5)
Anthropology of Work Review     Hybrid Journal   (Followers: 11, SJR: 0.256, h-index: 5)
Anthropology Today     Hybrid Journal   (Followers: 93, SJR: 0.545, h-index: 15)
Antipode     Hybrid Journal   (Followers: 44, SJR: 2.212, h-index: 69)
Anz J. of Surgery     Hybrid Journal   (Followers: 6, SJR: 0.432, h-index: 59)
Anzeiger für Schädlingskunde     Hybrid Journal   (Followers: 1)
Apmis     Hybrid Journal   (Followers: 1, SJR: 0.855, h-index: 73)
Applied Cognitive Psychology     Hybrid Journal   (Followers: 67, SJR: 0.754, h-index: 69)
Applied Organometallic Chemistry     Hybrid Journal   (Followers: 7, SJR: 0.632, h-index: 58)
Applied Psychology     Hybrid Journal   (Followers: 138, SJR: 1.023, h-index: 64)
Applied Psychology: Health and Well-Being     Hybrid Journal   (Followers: 48, SJR: 0.868, h-index: 13)
Applied Stochastic Models in Business and Industry     Hybrid Journal   (Followers: 5, SJR: 0.613, h-index: 24)
Aquaculture Nutrition     Hybrid Journal   (Followers: 13, SJR: 1.025, h-index: 55)
Aquaculture Research     Hybrid Journal   (Followers: 31, SJR: 0.807, h-index: 60)
Aquatic Conservation Marine and Freshwater Ecosystems     Hybrid Journal   (Followers: 34, SJR: 1.047, h-index: 57)
Arabian Archaeology and Epigraphy     Hybrid Journal   (Followers: 11, SJR: 0.453, h-index: 11)
Archaeological Prospection     Hybrid Journal   (Followers: 12, SJR: 0.922, h-index: 21)
Archaeology in Oceania     Hybrid Journal   (Followers: 13, SJR: 0.745, h-index: 18)
Archaeometry     Hybrid Journal   (Followers: 27, SJR: 0.809, h-index: 48)
Archeological Papers of The American Anthropological Association     Hybrid Journal   (Followers: 14, SJR: 0.156, h-index: 2)
Architectural Design     Hybrid Journal   (Followers: 25, SJR: 0.261, h-index: 9)
Archiv der Pharmazie     Hybrid Journal   (Followers: 4, SJR: 0.628, h-index: 43)
Archives of Drug Information     Hybrid Journal   (Followers: 4)
Archives of Insect Biochemistry and Physiology     Hybrid Journal   (SJR: 0.768, h-index: 54)
Area     Hybrid Journal   (Followers: 12, SJR: 0.938, h-index: 57)
Art History     Hybrid Journal   (Followers: 215, SJR: 0.153, h-index: 13)
Arthritis & Rheumatology     Hybrid Journal   (Followers: 49, SJR: 1.984, h-index: 20)
Arthritis Care & Research     Hybrid Journal   (Followers: 28, SJR: 2.256, h-index: 114)
Artificial Organs     Hybrid Journal   (Followers: 1, SJR: 0.872, h-index: 60)
ASHE Higher Education Reports     Hybrid Journal   (Followers: 14)
Asia & the Pacific Policy Studies     Open Access   (Followers: 14)
Asia Pacific J. of Human Resources     Hybrid Journal   (Followers: 313, SJR: 0.494, h-index: 19)
Asia Pacific Viewpoint     Hybrid Journal   (SJR: 0.616, h-index: 26)
Asia-Pacific J. of Chemical Engineering     Hybrid Journal   (Followers: 7, SJR: 0.345, h-index: 20)
Asia-pacific J. of Clinical Oncology     Hybrid Journal   (Followers: 6, SJR: 0.554, h-index: 14)
Asia-Pacific J. of Financial Studies     Hybrid Journal   (SJR: 0.241, h-index: 7)
Asia-Pacific Psychiatry     Hybrid Journal   (Followers: 3, SJR: 0.377, h-index: 7)
Asian Economic J.     Hybrid Journal   (Followers: 8, SJR: 0.234, h-index: 21)
Asian Economic Policy Review     Hybrid Journal   (Followers: 3, SJR: 0.196, h-index: 12)
Asian J. of Control     Hybrid Journal   (SJR: 0.862, h-index: 34)
Asian J. of Endoscopic Surgery     Hybrid Journal   (SJR: 0.394, h-index: 7)
Asian J. of Organic Chemistry     Hybrid Journal   (Followers: 4, SJR: 1.443, h-index: 19)
Asian J. of Social Psychology     Hybrid Journal   (Followers: 5, SJR: 0.665, h-index: 37)
Asian Politics and Policy     Hybrid Journal   (Followers: 13, SJR: 0.207, h-index: 7)
Asian Social Work and Policy Review     Hybrid Journal   (Followers: 5, SJR: 0.318, h-index: 5)
Asian-pacific Economic Literature     Hybrid Journal   (Followers: 5, SJR: 0.168, h-index: 15)
Assessment Update     Hybrid Journal   (Followers: 4)
Astronomische Nachrichten     Hybrid Journal   (Followers: 2, SJR: 0.701, h-index: 40)
Atmospheric Science Letters     Open Access   (Followers: 29, SJR: 1.332, h-index: 27)
Austral Ecology     Hybrid Journal   (Followers: 12, SJR: 1.095, h-index: 66)
Austral Entomology     Hybrid Journal   (Followers: 10, SJR: 0.524, h-index: 28)
Australasian J. of Dermatology     Hybrid Journal   (Followers: 8, SJR: 0.714, h-index: 40)
Australasian J. On Ageing     Hybrid Journal   (Followers: 7, SJR: 0.39, h-index: 22)
Australian & New Zealand J. of Statistics     Hybrid Journal   (Followers: 13, SJR: 0.275, h-index: 28)
Australian Accounting Review     Hybrid Journal   (Followers: 4, SJR: 0.709, h-index: 14)
Australian and New Zealand J. of Family Therapy (ANZJFT)     Hybrid Journal   (Followers: 3, SJR: 0.382, h-index: 12)
Australian and New Zealand J. of Obstetrics and Gynaecology     Hybrid Journal   (Followers: 43, SJR: 0.814, h-index: 49)
Australian and New Zealand J. of Public Health     Hybrid Journal   (Followers: 11, SJR: 0.82, h-index: 62)
Australian Dental J.     Hybrid Journal   (Followers: 6, SJR: 0.482, h-index: 46)
Australian Economic History Review     Hybrid Journal   (Followers: 4, SJR: 0.171, h-index: 12)
Australian Economic Papers     Hybrid Journal   (Followers: 22, SJR: 0.23, h-index: 9)
Australian Economic Review     Hybrid Journal   (Followers: 6, SJR: 0.357, h-index: 21)
Australian Endodontic J.     Hybrid Journal   (Followers: 3, SJR: 0.513, h-index: 24)
Australian J. of Agricultural and Resource Economics     Hybrid Journal   (Followers: 3, SJR: 0.765, h-index: 36)
Australian J. of Grape and Wine Research     Hybrid Journal   (Followers: 5, SJR: 0.879, h-index: 56)
Australian J. of Politics & History     Hybrid Journal   (Followers: 13, SJR: 0.203, h-index: 14)
Australian J. of Psychology     Hybrid Journal   (Followers: 17, SJR: 0.384, h-index: 30)
Australian J. of Public Administration     Hybrid Journal   (Followers: 386, SJR: 0.418, h-index: 29)
Australian J. of Rural Health     Hybrid Journal   (Followers: 4, SJR: 0.43, h-index: 34)
Australian Occupational Therapy J.     Hybrid Journal   (Followers: 66, SJR: 0.59, h-index: 29)
Australian Psychologist     Hybrid Journal   (Followers: 11, SJR: 0.331, h-index: 31)
Australian Veterinary J.     Hybrid Journal   (Followers: 19, SJR: 0.459, h-index: 45)
Autism Research     Hybrid Journal   (Followers: 31, SJR: 2.126, h-index: 39)
Autonomic & Autacoid Pharmacology     Hybrid Journal   (SJR: 0.371, h-index: 29)
Banks in Insurance Report     Hybrid Journal   (Followers: 1)
Basic & Clinical Pharmacology & Toxicology     Hybrid Journal   (Followers: 10, SJR: 0.539, h-index: 70)
Basic and Applied Pathology     Open Access   (Followers: 2, SJR: 0.113, h-index: 4)
Basin Research     Hybrid Journal   (Followers: 3, SJR: 1.54, h-index: 60)
Bauphysik     Hybrid Journal   (Followers: 2, SJR: 0.194, h-index: 5)
Bauregelliste A, Bauregelliste B Und Liste C     Hybrid Journal  
Bautechnik     Hybrid Journal   (Followers: 1, SJR: 0.321, h-index: 11)
Behavioral Interventions     Hybrid Journal   (Followers: 8, SJR: 0.297, h-index: 23)
Behavioral Sciences & the Law     Hybrid Journal   (Followers: 23, SJR: 0.736, h-index: 57)
Berichte Zur Wissenschaftsgeschichte     Hybrid Journal   (Followers: 9, SJR: 0.11, h-index: 5)
Beton- und Stahlbetonbau     Hybrid Journal   (Followers: 2, SJR: 0.493, h-index: 14)
Biochemistry and Molecular Biology Education     Hybrid Journal   (Followers: 6, SJR: 0.311, h-index: 26)
Bioelectromagnetics     Hybrid Journal   (Followers: 1, SJR: 0.568, h-index: 64)
Bioengineering & Translational Medicine     Open Access  
BioEssays     Hybrid Journal   (Followers: 10, SJR: 3.104, h-index: 155)
Bioethics     Hybrid Journal   (Followers: 14, SJR: 0.686, h-index: 39)
Biofuels, Bioproducts and Biorefining     Hybrid Journal   (Followers: 1, SJR: 1.725, h-index: 56)
Biological J. of the Linnean Society     Hybrid Journal   (Followers: 14, SJR: 1.172, h-index: 90)
Biological Reviews     Hybrid Journal   (Followers: 3, SJR: 6.469, h-index: 114)
Biologie in Unserer Zeit (Biuz)     Hybrid Journal   (Followers: 42, SJR: 0.12, h-index: 1)
Biology of the Cell     Full-text available via subscription   (Followers: 9, SJR: 1.812, h-index: 69)
Biomedical Chromatography     Hybrid Journal   (Followers: 6, SJR: 0.572, h-index: 49)
Biometrical J.     Hybrid Journal   (Followers: 5, SJR: 0.784, h-index: 44)
Biometrics     Hybrid Journal   (Followers: 36, SJR: 1.906, h-index: 96)
Biopharmaceutics and Drug Disposition     Hybrid Journal   (Followers: 10, SJR: 0.715, h-index: 44)
Biopolymers     Hybrid Journal   (Followers: 18, SJR: 1.199, h-index: 104)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 45, SJR: 0.415, h-index: 55)
Biotechnology and Bioengineering     Hybrid Journal   (Followers: 136, SJR: 1.633, h-index: 146)
Biotechnology J.     Hybrid Journal   (Followers: 13, SJR: 1.185, h-index: 51)
Biotechnology Progress     Hybrid Journal   (Followers: 39, SJR: 0.736, h-index: 101)
Biotropica     Hybrid Journal   (Followers: 18, SJR: 1.374, h-index: 71)
Bipolar Disorders     Hybrid Journal   (Followers: 10, SJR: 2.592, h-index: 100)
Birth     Hybrid Journal   (Followers: 34, SJR: 0.763, h-index: 64)
Birth Defects Research Part A : Clinical and Molecular Teratology     Hybrid Journal   (Followers: 2, SJR: 0.727, h-index: 77)
Birth Defects Research Part B: Developmental and Reproductive Toxicology     Hybrid Journal   (Followers: 5, SJR: 0.468, h-index: 47)
Birth Defects Research Part C : Embryo Today : Reviews     Hybrid Journal   (SJR: 1.513, h-index: 55)

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Journal Cover Annals of Applied Biology
  [SJR: 0.816]   [H-I: 56]   [8 followers]  Follow
    
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0003-4746 - ISSN (Online) 1744-7348
   Published by John Wiley and Sons Homepage  [1583 journals]
  • The role of domestication and maternal effects on seed traits of
           crop–wild sunflower hybrids (Helianthus annuus)
    • Authors: F. Hernández; L.I. Lindström, E. Parodi, M. Poverene, A. Presotto
      Abstract: Hybridisation between crops and their wild relatives may promote the evolution of weeds. Seed germination and dormancy are the earliest life-history traits and are highly influenced by the maternal parent. However, the ecological role of the maternal effect on seed traits in the evolution of crop–wild hybrids has received little attention. In this study, we test the relative importance of maternal and hybridisation effects on seed traits of the first generation of crop–wild sunflower hybrids (Helianthus annuus). Seed germination was tested in two wild populations with contrasting dormancy, two cultivated materials and their reciprocal crosses at four different times after harvest and three different temperatures. Seed germination at each of the four times, after ripening response and secondary dormancy were recorded along with four morphological traits. Additionally, the pericarp anatomy was analysed with light and scanning electron microscopy. We observed strong maternal effects on all seed traits. Seed germination, morphology and pericarp anatomy differed largely between the crop and wild seeds and these traits in the crop–wild hybrids resembled their female parent. Slight but significant hybridisation effects were observed in germination, mainly in seeds produced on wild plants. Crop hybridisation changed seed germination, the after ripening response and secondary dormancy in the crop direction. Morphological and anatomical traits associated with domestication strongly correlated with the observed differences in seed germination and dormancy in crop–wild sunflower hybrids. The large maternal effects along with the evolutionary divergence in seed traits were responsible for the large phenotypic differences observed in crop–wild hybrids with the same genetic composition. Wild-like seed traits of hybrids suggest that there are no barriers to crop gene introgression at the seed level whereas crop-like seed traits could be strongly selected against, conditioning the selection of traits expressed later in the life cycle and in the next generations.
      PubDate: 2017-06-15T00:35:35.230304-05:
      DOI: 10.1111/aab.12368
       
  • Seed treatment with selected plant growth-promoting rhizobacteria
           increases maize yield in the field
    • Authors: G. Breedt; N. Labuschagne, T.A. Coutinho
      Abstract: Maize, Zea mays is the most important grain crop in South Africa and is a staple food in many African countries. The beneficial effects of plant growth-promoting rhizobacteria on crop growth and yield have been well documented, but obtaining reproducible results under field conditions is often difficult. In the current study, five selected rhizobacterial strains that showed plant growth-promoting activities in pilot studies were evaluated for potential enhancement of maize yield under field conditions. The five strains together with a commercial standard were assessed as seed treatments of maize over three seasons in four different soil types. The strains were identified on the basis of 16S rRNA sequencing as Lysinibacillus sphaericus (T19), Paenibacillus alvei (T29), Bacillus safensis (S7) Bacillus pumilus (A26) and Brevundimonas vesicularis (A40). The best yield increases in maize were obtained during the 2011/2012 and 2012/2013 seasons in the Shortlands ecotope with the rhizobacterial strains T19, T29 and S7, resulting in yield increases ranging from 24% to 34%. Strain T19 rendered the most consistent yield increases during the three successive field trials amounting to 33% and 24% in Shortlands ecotope and 12% in Clovalley ecotope, respectively. During 2013/2014 a consortium of three strains, viz. T19, S7 and A26 gave a 32% yield increase in Clovalley ecotope. All the rhizobacterial strains solubilised phosphate in vitro except T19. Strain T29 showed the best nitrogen-fixing activity in vitro, proliferating on a nitrogen-free substrate and also producing ammonia. All the strains tested positive for indole acetic acid production. The current study demonstrates the ability of rhizobacterial strains T19, T29, S7 and A26 applied as seed treatments to significantly enhance maize yield in the field, making development and commercialisation of these strains a viable option.
      PubDate: 2017-06-15T00:35:21.83683-05:0
      DOI: 10.1111/aab.12366
       
  • A discussion on disease severity index values. Part I: warning on inherent
           errors and suggestions to maximise accuracy
    • Authors: K.S. Chiang; H.I. Liu, C.H. Bock
      Abstract: A special type of ordinal scale comprising a number of intervals of known numeric ranges can be used when estimating severity of a plant disease. The interval ranges are most often based on the percent area with symptoms [e.g. the Horsfall–Barratt (H–B) scale]. Studies in plant pathology and plant breeding often use this type of ordinal scale. The disease severity is estimated by a rater as a value on the scale and has been used to determine a disease severity index (DSI) on a percentage basis, where DSI (%) = [sum (class frequency × score of rating class)]/[(total number of plants) × (maximal disease index)] × 100. However, very few studies have investigated the effects of different scales on accuracy of the DSI. Therefore, the objectives of this study were to investigate the process of calculating a DSI on a percentage basis from ordinal scale data, and to use simulation approaches to explore the effect of using different methods for calculation of the interval range and the nature of the ordinal scales used on the DSI estimates (%). We found that the DSI is particularly prone to overestimation when using the above formula if the midpoint values of the rating class are not considered. Moreover, the results of the simulation studies show that, if rater estimates are unbiased, compared with other methods tested in this study, the most accurate method for estimation of a DSI is to use the midpoint of the severity range for each class with an amended 10% ordinal scale (an ordinal scale based on a 10% linear scale emphasising severities ≤50% disease, with additional grades at low severities). As for biased conditions, the accuracy for calculating DSI estimates (%) will depend mainly on the degree and direction of the rater bias relative to the actual mean value.
      PubDate: 2017-06-06T03:20:39.04349-05:0
      DOI: 10.1111/aab.12362
       
  • Persistence of transgenes in wild rice populations depends on the
           interaction between genetic background of recipients and environmental
           conditions
    • Authors: S.S. Dong; M.Q. Xiao, D.X. Ouyang, J. Rong, B.-R. Lu, J. Su, F. Wang, J.K. Chen, Z.-P. Song
      Abstract: The persistence of transgenes in wild populations may cause unintended ecological consequences, and the possibility of transgenes' persistence and introgression is dependent on fitness performance of transgenic crop–wild hybrids. To investigate the effects of transgene and genotype × environment on the fitness of crop–wild rice hybrids, a total of 11 cross-combination progenies between insect-resistant transgene (CpTI and Bt/CpTI) rice lines and wild rice (Oryza rufipogon) were evaluated at different sites with contrasting insect treatments. The results showed that fitness performance varied between transgenic hybrids having different wild parents and under different environmental conditions, indicating that fitness effects of transgenes on hybrid progenies depend heavily on the genetic background of recipient plants and growing environment. Significant fitness advantages conferred by transgenes were found only in some hybrids under high insect pressure condition, demonstrating that the level of target insects in the field environment influences the persistence and spread of insect-resistant transgenes in wild rice populations. These findings suggest that evolutionary fate of escaped transgenes is different in wild populations with diverse genetic backgrounds under various environmental conditions.
      PubDate: 2017-06-01T02:50:28.325977-05:
      DOI: 10.1111/aab.12365
       
  • Probability distributions for marketable pods and white mould on snap bean
    • Authors: D.A. Shah; H.R. Dillard, S.J. Pethybridge
      Abstract: White mould, caused by Sclerotinia sclerotiorum, is one of the most recalcitrant diseases of snap bean. Probability distributions suitable for describing the total number of marketable pods (hereafter simply referred to as pods) per plant, as well as for the number of pods and stems with white mould per plant, have not been identified. The total number of pods and the number of pods and stems with white mould were measured on a per plant basis in plots of processing snap bean (var. Hystyle) in New York. The total number of pods per plant ranged from 0 to 29, and was best described by the Pólya-Aeppli distribution. The number of pods and the number of stems with white mould per plant were well-described by the negative binomial (NB) distribution. A Sarmanov bivariate distribution with NB marginals was derived and fitted to the joint data on the number of pods and stems with white mould per plant, accounting for correlation between pods and stems with white mould on the same plant. The bivariate distribution was used to formulate an empirical equation for the incidence of plants with white mould as a function of the average number of pods and stems with white mould per plant. The results represented a more complete understanding of the distributional properties of white mould in snap bean.
      PubDate: 2017-05-23T04:11:24.960644-05:
      DOI: 10.1111/aab.12363
       
  • Ethylenediamine-N,N′-disuccinic acid mitigates salt-stress damages in
           strawberry by interfering with effects on the plant ionome
    • Authors: R. Aslantas; I. Angin, M. Kose, N. Bernstein
      Abstract: This study evaluated the hypothesis that the organic chelant ethylenediamine-N,N′-disuccinic acid (EDDS) mitigates plant damage under salinity, and that this is accomplished by EDDS-induced effects on cation uptake. Damaging effects of salinity on plants often involve inhibited uptake of nutritional cations, such as K and Ca, and excessive accumulation of Na. Therefore, mechanisms that improve uptake of K and Ca, or reduce Na uptake, have a potential for ameliorating salinity damages. Organic chelants increase heavy-metal cation availability at the site of uptake and increase their uptake by the roots or in planta transport. Although organic chelants are routinely used in agriculture to enhance uptake of heavy-metal cations into plants, and for soil bioremediation, their effect on uptake of cation-macronutrients is not known, and neither is their impact on plant function under salinity. In this study, we evaluated the response of strawberry plants to EDDS application (0, 1, 3 and 5 mmol kg soil−1), under six levels of NaCl (0, 3, 6, 9, 12 and 15 mmol L−1). EDDS application under salinity improved vegetative development, as well as reproductive growth and chlorophyll content, with statistically significant interaction between chelant dosage and level of salinity. The mitigation of salinity damage by EDDS occurred at high salinity treatments (from 9 mM NaCl). Application rates of 1–3 mmol EDDS kg−1 were optimal for mitigating salinity effects on reproductive development, but in accordance with the extent of chelant-induced accumulation of the macronutrients K, Ca and P in the leaves, higher application rates (3–5 mmol EDDS kg−1) were required for optimal improvement of vegetative development. These results suggest that EDDS improves plant function under mild salinities by interfering with salinity effects on the plant ionome.
      PubDate: 2017-05-23T03:40:29.654703-05:
      DOI: 10.1111/aab.12364
       
  • Effects of Grapevine leafroll-associated virus 3 on the physiology in
           asymptomatic plants of Vitis vinifera
    • Authors: R. Montero; H. El aou ouad, D. Pacifico, C. Marzachì, N. Castillo, E. García, N.F. Del Saz, I. Florez-Sarasa, J. Flexas, J. Bota
      Abstract: Grapevine leafroll disease is one of the most important viral diseases of grapevine (Vitis vinifera) worldwide. Grapevine leafroll-associated virus 3 (GLRaV-3) is the most predominant virus species causing this disease. Therefore, it is important to identify GLRaV-3 effects, especially in plants which do not systematically show visual symptoms. In this study, effects of GLRaV-3 on grapevine physiology were evaluated in asymptomatic plants of Malvasía de Banyalbufar and Cabernet Sauvignon cvs. Absolute virus quantification was performed in order to determine the level of infection of the treatment. The net carbon dioxide (CO2) assimilation (AN) and electron transport rate (Jflux) were the main parameters affected by the virus. The AN reduction in infected plants was attributed to restrictions in CO2 diffusion caused by anatomical leaf changes and a reduction of Rubisco activity. Those effects were more evident in Malvasia de Banyalbufar plants. The reduction of AN leads to a decrease in the total oxygen uptake rate by the activity of the cytochrome oxidase pathway, producing slight differences in plant growth. Therefore, even though no symptoms were expressed in the plants, the effects of the virus compromised the plant vital processes, showing the importance of early detection of the virus in order to fight against the infection.
      PubDate: 2017-05-17T03:41:11.103456-05:
      DOI: 10.1111/aab.12356
       
  • Lessons learned from the virus indexing of Musa germplasm: insights from a
           multiyear collaboration
    • Authors: C. De Clerck; K. Crew, I. Van den houwe, L. McMichael, C. Berhal, L. Lassois, M. Haissam Jijakli, N. Roux, J. Thomas, S. Massart
      Abstract: The Bioversity International Transit Center (ITC) for banana hosts more than 1500 accessions largely covering the genetic diversity of the genus Musa. Its objective is to conserve this genetic diversity and to supply plant materials to users worldwide. All the Musa accessions must be tested for virus presence and, if infected, virus elimination must be attempted, to enable the supply of virus-free plant material. An international collaborative effort launched under the auspices of Bioversity International (2007–2013) finally led to the implementation of a two-step process to test the accessions. The first step, called pre-indexing, involved only molecular tests and was designed as a pre-screen of new germplasm lines or existing accessions to reduce the need for post-entry virus therapy and repeated virus indexing. The second step, called full indexing, was performed on either older existing accessions or newer accessions which tested negative during pre-indexing, and involved molecular tests, transmission electron microscopy (TEM) and symptom observation. In total, 270 germplasm lines (434 samples) were pre-indexed; while full indexing was carried out on 243 accessions (68 of which had been pre-indexed). A significant proportion of the samples tested during pre-indexing was infected with at least one virus (68%), showing the utility of this early pre-screening step. Banana streak OL virus and Banana mild mosaic virus were the most commonly detected viruses during both pre- and full indexing. For 22 accessions, viral particles were observed by TEM in full indexing while the molecular tests were negative, underlining the importance of combining various detection techniques. After full indexing, viruses were not detected in 166 accessions, which were then released for international distribution from the ITC. This publication exemplifies how the practical application of diagnostic protocols can raise fundamental questions related to their appropriate use in routine practice and the need for their continuous monitoring and improvement after their first publication.
      PubDate: 2017-04-20T04:51:31.242832-05:
      DOI: 10.1111/aab.12353
       
  • Allometric equations for yield predictions of enset (Ensete ventricosum)
           and khat (Catha edulis) grown in home gardens of southern Ethiopia
    • Authors: B.T. Mellisse; K. Descheemaeker, M.J. Mourik, G.W.J. Ven
      Abstract: Enset is a large, single-stemmed perennial herbaceous plant domesticated as a staple food crop only in Ethiopia. Khat is a perennial plant cultivated for its economically important leaves and twigs that are the sources of stimulant when chewed. We address the issue of yield estimation of both crops, as they are important for the livelihoods of smallholders in the home garden systems in Southern Ethiopia and have received little attention so far. The objective of this study was to develop linear allometric models for estimating the edible (food and feed) and commercial yields of enset and khat plants, respectively. Data were collected from 20 enset and 100 khat plants. Diameter at 50-cm height (d50), pseudostem height (hp) and their combination were good predictor variables for the food products of enset with adjusted R2 values above 0.85, while d50, hp, edible pseudostem height (hep), total height (ht) and their combination were good predictor variables for the feed products of enset with adjusted R2 values above 0.70. For dwarf khat plants crown area (ca) combined with total height (ht) resulted in the best prediction with an adjusted R2 of 0.77, while the leaf and twig dry weight for tall khat plants was best predicted by ca with adjusted R2 of 0.43. In all cases linear models were used.
      PubDate: 2017-04-18T04:05:49.499485-05:
      DOI: 10.1111/aab.12350
       
  • Effects of species and soil-nitrogen availability on root system
           architecture traits – study on a set of weed and crop species
    • Authors: D. Moreau; F. Abiven, H. Busset, A. Matejicek, L. Pagès
      Abstract: Better managing crop : weed competition in cropping systems while reducing both nitrogen and herbicide inputs is a real challenge that requires a better understanding of crop and weed root architecture in relation to soil-nitrogen availability. An original approach was used which considered the parameters of a simulation model of root architecture as traits to analyse (a) the interspecific diversity of root system architecture, and (b) its response to soil-nitrogen availability. Two greenhouse experiments were conducted using three crop and nine weed species grown at two contrasted concentrations of soil-nitrogen availability. Plant traits were measured to characterise both overall plant growth and root architecture, with a focus on primary root emergence, root elongation and branching. The studied root traits varied among species (from a twofold to a fourfold factor, depending on the trait), validating their use as indicators to analyse the interspecific variability of root architecture. The largest interspecies differences were for two traits: ‘maximal apical root diameter’ and ‘interbranch distance’ (distance between two successive laterals on the same root). Conversely, most of the studied root traits varied little with soil-nitrogen availability (from no variation to a 1.1-fold factor, depending on the trait) even though soil-nitrogen availability varied with a 17-fold factor and impacted the overall shoot and root biomass. So, the root traits used in this article are stable whatever soil-nitrogen availability. As they reflect processes underlying root system architecture, this low effect of nitrogen suggests that the rules governing root architecture are little affected by plant nitrogen status and soil-nitrogen availability. We propose that the determinants of differences in root system architecture between soils with contrasted nitrogen availability mainly originate from differences in the amount of carbon allocated to and within the root system. Characterising each plant species by a combination of root traits gave insights regarding the potential species competitive ability for soil resources in agroecosystems.
      PubDate: 2017-04-11T03:10:29.020179-05:
      DOI: 10.1111/aab.12355
       
  • New insights on Flavescence dorée phytoplasma ecology in the vineyard
           agro-ecosystem in southern Switzerland
    • Authors: P. Casati; M. Jermini, F. Quaglino, G. Corbani, S. Schaerer, A. Passera, P.A. Bianco, I.E. Rigamonti
      Abstract: Phytoplasmas associated with Flavescence dorée (FDp) grapevine disease are quarantine pathogens controlled through mandatory measures including the prompt eradication and destruction of diseased plants, and the insecticide treatments against the insect vector, the ampelophagous leafhopper Scaphoideus titanus. In the present study, a multidisciplinary approach has been applied to investigate the FDp ecological cycle in a test vineyard agro-ecosystem in Canton Ticino, south Switzerland. Despite the scarce population density of S. titanus, a regular trend of new infections (3.4% of the total vines) through the years was observed. The leafhopper Orientus ishidae was found as the most abundant among the captured insect species known as phytoplasma vectors (245 out of 315 specimens). The population of O. ishidae was evidenced prevalently (167 specimens) in the south-western side of the vineyard and within the neighbouring forest constituted mainly by hazel (Corylus avellana) and willow (Salix spp.). These plant species were found infected by FDp related strains (30% of analysed trees) for the first time in this study. Interestingly, O. ishidae was found to harbour FDp related strains in high percentage (26% of the analysed pools). In addition, 16SrV phytoplasma group was detected for the first time in the insect Hyalesthes obsoletus and a FDp related strain in Thamnotettix dilutior, present in low populations within the test vineyard. Molecular characterisation and phylogenetic analyses of methionine aminopeptidase (map) gene sequences of FDp and related strains, here identified, revealed the great prevalence of the map-type FD2 in grapevines (97%) and in O. ishidae pools (72%). Such a map-type was found also in hazel and in T. dilutior, but not in S. titanus. Moreover, map-types FD1 and FD3 were identified for the first time in Switzerland in several host plants and phytoplasma vectors, including grapevine (FD1), S. titanus (FD1) and O. ishidae (FD1 and FD3). Based on the data obtained in this study, it is reasonable to hypothesise that the ecological cycle of FDp could be related not exclusively to the grapevine-specific feeding diet of S. titanus, but it could include other insect vector(s) and/or plant host(s). Further studies will be needed to prove the role of O. ishidae as vector able to transmit FDp from wild plants (e.g. hazel) to grapevine.
      PubDate: 2017-04-07T04:38:33.379387-05:
      DOI: 10.1111/aab.12359
       
  • A real-time PCR assay for improved rapid, specific detection of
           Cryphonectria parasitica
    • Authors: S. Rubio; A. Barnes, K. Webb, J. Hodgetts
      Abstract: Cryphonectria parasitica, an ascomycete fungus, is the causal agent of chestnut blight. This highly destructive disease of chestnut trees causes significant losses, and is therefore a regulated pathogen in Europe. Existing methods for the detection of C. parasitica include morphological identification following culturing, or PCR; however, these are time-consuming resulting in delays to diagnosis. To allow improved detection, a new specific real-time PCR assay was designed to detect C. parasitica directly from plant material and fungal cultures, and was validated according to the European Plant Protection Organisation (EPPO) standard PM 7/98. The analytical specificity of the assay was tested extensively using a panel of species taxonomically closely related to Cryphonectria, fungal species associated with the hosts and healthy plant material. The assay was found to be specific to C. parasitica, whilst the analytical sensitivity of the assay was established as 2 pg µL−1 of DNA. Comparative testing of 63 samples of naturally infected plant material by the newly developed assay and traditional morphological diagnosis demonstrated an increased diagnostic sensitivity when using the real-time PCR assay. Furthermore the assay is able to detect both virulent and hypovirulent strains of C. parasitica. Therefore the new real-time PCR assay can be used to provide reliable, rapid, specific detection of C. parasitica to prevent the accidental movement of the disease and to monitor its spread.
      PubDate: 2017-04-07T04:01:37.548846-05:
      DOI: 10.1111/aab.12354
       
  • First report of the gall midge Asphondylia serpylli on thyme (Thymus
           vulgaris), and identification of the associated fungal symbiont
    • Authors: B. Zimowska; G. Viggiani, R. Nicoletti, A. Furmańczyk, A. Becchimanzi, I. Kot
      Abstract: Asphondylia spp. (Diptera: Cecidomyiidae) are known for inducing gall formation on many diverse plants in both wild and agricultural contexts. The species Asphondylia serpylli is herewith reported for the first time on thyme (Thymus vulgaris) cropped in Poland. The associated fungus has been identified as Botryosphaeria dothidea, representing its first record from cecidomyiid galls on a species of Lamiaceae. Moreover, a short account is given on the parasitoid species active in this particular ecological context. These findings point out the basic role of B. dothidea in the organization of these three-component biotic systems regardless of the varied assortments between the midge species and their host plant.
      PubDate: 2017-03-31T06:35:25.306197-05:
      DOI: 10.1111/aab.12360
       
  • Influence of limiting and regulating factors on populations of Asian
           citrus psyllid and the risk of insect and disease outbreaks
    • Authors: B.J. Udell; C. Monzo, T.M. Paris, S.A. Allan, P.A. Stansly
      Abstract: Diaphorina citri, known as the Asian citrus psyllid (ACP), is the insect vector of a devastating citrus disease (huanglongbing; HLB), which has caused billions of dollars in damage in Florida since its detection in 2005. Data from long-term monitoring programs in two Florida citrus groves were used to assess ACP demography and population ecology, which is needed to implement more effective management strategies for HLB. Seasonal patterns and correlations between ACP density estimates and a suite of environmental and community indicators, previously shown to influence ACP demography, were described and interpreted. Data were evaluated for evidence for spatial clustering and density-dependent recruitment of ACP in a major outbreak event using Taylor power law analysis and by fitting a stochastic Beverton–Holt recruitment model using state-space approach. Strong evidence for density-dependent recruitment and spatial clustering was found and should be considered for future modelling work as it can greatly influence ACP populations by affecting their growth rates, dispersal behaviour and morphology, as well as HLB transmission. Observations of ACP density and dispersion in space and time, along with the estimated parameters from the Beverton–Holt model, suggested that intraspecific competition for resources may initiate both local, then widespread dispersal process, thus affecting grove-wide and area-wide HLB transmission. When these results were synthesised with those of parallel studies, the removal of several of these regulating factors in a single year could lead to widespread disease of the entire crop in a grove, and likely surrounding groves as well. We provide the first field evidence of the consequences of back-to-back ACP colonisation on the rapid spread of the HLB in a grove (a mechanism previously demonstrated in laboratory settings). We stress the importance of proper integrated pest management and area-wide management to prevent such outbreaks.
      PubDate: 2017-03-31T06:30:43.440895-05:
      DOI: 10.1111/aab.12349
       
  • Effect of five fungicides with different modes of action on cobweb disease
           (Cladobotryum mycophilum) and mushroom yield
    • Authors: J. Carrasco; M.J. Navarro, M. Santos, F.J. Gea
      Abstract: The fungicides chlorothalonil, metrafenone, prochloraz-Mn, thiabendazole and thiophanate-methyl were tested in vitro and in vivo for their effect on Cladobotryum mycophilum, the mycoparasite that causes cobweb disease in white button mushroom. In vitro experiments showed that metrafenone (EC50= 0.025 mg L−1) and prochloraz-Mn (EC50= 0.045 mg L−1) were the most effective fungicides for inhibiting the mycelial growth of C. mycophilum. Selectivity indexes of the tested fungicides on both C. mycophilum and Agaricus bisporus indicated that metrafenone was also the most selective fungicide, while chlorothalonil was the most toxic fungicide against A. bisporus mycelium. The in vivo efficacy of fungicides for controlling cobweb was evaluated in three mushroom cropping trials, which were artificially inoculated with C. mycophilum (106 conidia m−2). Prochloraz-Mn provided good control, although the surface colonised by cobweb reached 12% by the end of the crop cycles. None of the inoculated cropping trials treated with metrafenone showed any cobweb disease symptoms, and neither were any significant phytotoxic effects on mushroom yield recorded. These results indicated that metrafenone can be used as an alternative to prochloraz-Mn in the control of cobweb disease.
      PubDate: 2017-03-31T06:20:24.830082-05:
      DOI: 10.1111/aab.12352
       
  • Phenological growth stages of quinoa (Chenopodium quinoa) based on the
           BBCH scale
    • Authors: V. Sosa-Zuniga; V. Brito, F. Fuentes, U. Steinfort
      Abstract: Quinoa (Chenopodium quinoa) is a pseudocereal native from the Andean region of South America that has increased in importance worldwide. Quinoa is now considered an alternative to traditional crops in a climate change scenario, considering its ability to adapt to marginal soils, droughts and frosts. Despite the interesting agronomic and nutritional features of this crop, research into quinoa is characterised by individual attempts to define its phenological stages without an international consensus. A unique criterion to quantify the phenology of quinoa could become a useful tool for researchers and plant breeders in future work by standardising this information for international cooperation. In this article, a proposed scale of the phenological growth stages of quinoa based on the BBCH coding system (Biologische Bundesanstalt Bundessortenamt und CHemische Industrie) was developed. Growth stages were described utilising the decimal code of the BBCH system, and figures were included for the most representative stages.
      PubDate: 2017-03-31T06:15:43.248042-05:
      DOI: 10.1111/aab.12358
       
  • Temporal and spatial dynamics of Tomato spotted wilt virus and its vector
           in a potato crop in Argentina
    • Authors: A.E. Salvalaggio; P.M. López Lambertini, G. Cendoya, M.A. Huarte
      Abstract: The nature of spatial and temporal dynamics of Tomato spotted wilt virus (TSWV) and its vector in a potato crop cv. Innovator without insecticide application is analysed. Seed tuber was analysed for the presence of TSWV as a source of initial inoculum. The presence of plants with symptoms of TSWV was evaluated by visual observation and DAS-ELISA analysis to confirm the virus infection. Thrips species were collected from leaves and inflorescences and identified under stereomicroscope. The distribution of symptomatic plants and thrips species was recorded five times at 14 days intervals. The initial seed tuber infection was of 1.1%. Disease incidence was 0% at 29 days after planting (DAP), 0.2% at 43 DAP, 2.2% at 56 DAP, 11.6% at 70 DAP and 14.6% at 84 DAP. The progress of the disease was adequately described by a Logistic model [y = 0.15/(1 + 1205372.93 × exp (−0.22 × DAP))]. Thrips vector species identified as resident in the crop during the whole cycle were Thrips tabaci (n = 423), Frankliniella occidentalis (n = 141) and as occasional species, F. schultzei (n = 34) and F. gemina (n = 5) were found. At 43 and 56 DAP a random distribution pattern was observed and the thrips species found were T. tabaci (n = 188) and F. occidentalis (n = 105). An aggregated pattern was determined at 70 and 84 DAP. Spatial patterns of the disease spread suggest a polycyclic epidemic with TSWV secondary spread in the potato crop. Multiple control measures were deduced from these epidemiological results like virus testing in tubers, removal of external virus infection sources and thrips control.
      PubDate: 2017-03-28T03:30:26.533028-05:
      DOI: 10.1111/aab.12357
       
  • Detection of lethal yellowing phytoplasma in coconut plantlets obtained
           through in vitro germination of zygotic embryos from the seeds of infected
           palms
    • Authors: C. Oropeza; I. Cordova, C. Puch-Hau, R. Castillo, J.L. Chan, L. Sáenz
      Abstract: Lethal yellowing (LY) is a disease caused by 16SrIV phytoplasmas that has devastated coconut plantations in the Americas. An alternative means of phytoplasma spread is through seeds. Therefore, we used a novel approach based on plumules from the embryos of LY-diseased coconut palms. We cultured the plumules in vitro to determine the presence of phytoplasma DNA in the plantlets. In the first assay, 185 embryos were obtained. The results showed positive detection in 20 samples (11%) with the nested PCR and in 59 samples (32%) with the TaqMan real-time PCR. A second assay was designed to trace plumules to their respective embryos and haustorial tissues to determine whether they had derived from an embryo with positive LY detection; a total of 124 embryos were obtained. The results showed no positive detection with the nested PCR and positive detection in 42 of the haustorial tissue samples (32%) with the TaqMan real-time PCR. The 124 plumules isolated from the embryos were cultivated under in vitro conditions and divided into two groups. Group A was followed for shoot formation and Group B was followed to the plantlet stage. After 3 months of cultivation, 33 cultures (50%) within Group A became necrotic; the rest were analysed to evaluate LY phytoplasma DNA with the TaqMan real-time PCR assay and 14 (42%) tested positive. After 18 months of cultivation, 20 cultures (34%) within Group B became necrotic. The rest were analysed for the detection of the LY phytoplasma DNA, and 15 and 11 (39% and 29%) of the samples tested positive with the TaqMan real-time PCR and nested PCR assays, respectively. Blast analysis of the sequenced products revealed that the sequences showed 99% homology with LY-phytoplasma subgroup 16SrIV-A. The results presented here demonstrate, for the first time, the occurrence of the transmission of LY phytoplasmas from coconut embryos to plantlets.
      PubDate: 2017-03-14T02:05:38.907363-05:
      DOI: 10.1111/aab.12351
       
  • Ecology and diversity of plant viruses, and epidemiology of plant
           virus-induced diseases
    • Authors: M.A. Aranda; J. Freitas-Astúa
      Pages: 1 - 4
      PubDate: 2017-06-05T05:37:43.914437-05:
      DOI: 10.1111/aab.12361
       
 
 
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