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Publisher: John Wiley and Sons   (Total: 1592 journals)

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Showing 1 - 200 of 1592 Journals sorted alphabetically
Abacus     Hybrid Journal   (Followers: 13, SJR: 0.48, h-index: 22)
About Campus     Hybrid Journal   (Followers: 5)
Academic Emergency Medicine     Hybrid Journal   (Followers: 66, SJR: 1.385, h-index: 91)
Accounting & Finance     Hybrid Journal   (Followers: 47, SJR: 0.547, h-index: 30)
ACEP NOW     Free   (Followers: 1)
Acta Anaesthesiologica Scandinavica     Hybrid Journal   (Followers: 54, SJR: 1.02, h-index: 88)
Acta Archaeologica     Hybrid Journal   (Followers: 171, SJR: 0.101, h-index: 9)
Acta Geologica Sinica (English Edition)     Hybrid Journal   (Followers: 3, SJR: 0.552, h-index: 41)
Acta Neurologica Scandinavica     Hybrid Journal   (Followers: 5, SJR: 1.203, h-index: 74)
Acta Obstetricia et Gynecologica Scandinavica     Hybrid Journal   (Followers: 14, SJR: 1.197, h-index: 81)
Acta Ophthalmologica     Hybrid Journal   (Followers: 6, SJR: 0.112, h-index: 1)
Acta Paediatrica     Hybrid Journal   (Followers: 56, SJR: 0.794, h-index: 88)
Acta Physiologica     Hybrid Journal   (Followers: 6, SJR: 1.69, h-index: 88)
Acta Polymerica     Hybrid Journal   (Followers: 9)
Acta Psychiatrica Scandinavica     Hybrid Journal   (Followers: 37, SJR: 2.518, h-index: 113)
Acta Zoologica     Hybrid Journal   (Followers: 7, SJR: 0.459, h-index: 29)
Acute Medicine & Surgery     Hybrid Journal   (Followers: 5)
Addiction     Hybrid Journal   (Followers: 36, SJR: 2.086, h-index: 143)
Addiction Biology     Hybrid Journal   (Followers: 15, SJR: 2.091, h-index: 57)
Adultspan J.     Hybrid Journal   (SJR: 0.127, h-index: 4)
Advanced Energy Materials     Hybrid Journal   (Followers: 26, SJR: 6.411, h-index: 86)
Advanced Engineering Materials     Hybrid Journal   (Followers: 26, SJR: 0.81, h-index: 81)
Advanced Functional Materials     Hybrid Journal   (Followers: 51, SJR: 5.21, h-index: 203)
Advanced Healthcare Materials     Hybrid Journal   (Followers: 14, SJR: 0.232, h-index: 7)
Advanced Materials     Hybrid Journal   (Followers: 279, SJR: 9.021, h-index: 345)
Advanced Materials Interfaces     Hybrid Journal   (Followers: 6, SJR: 1.177, h-index: 10)
Advanced Optical Materials     Hybrid Journal   (Followers: 7, SJR: 2.488, h-index: 21)
Advanced Science     Open Access   (Followers: 5)
Advanced Synthesis & Catalysis     Hybrid Journal   (Followers: 18, SJR: 2.729, h-index: 121)
Advances in Polymer Technology     Hybrid Journal   (Followers: 13, SJR: 0.344, h-index: 31)
Africa Confidential     Hybrid Journal   (Followers: 21)
Africa Research Bulletin: Economic, Financial and Technical Series     Hybrid Journal   (Followers: 13)
Africa Research Bulletin: Political, Social and Cultural Series     Hybrid Journal   (Followers: 11)
African Development Review     Hybrid Journal   (Followers: 33, SJR: 0.275, h-index: 17)
African J. of Ecology     Hybrid Journal   (Followers: 16, SJR: 0.477, h-index: 39)
Aggressive Behavior     Hybrid Journal   (Followers: 16, SJR: 1.391, h-index: 66)
Aging Cell     Open Access   (Followers: 11, SJR: 4.374, h-index: 95)
Agribusiness : an Intl. J.     Hybrid Journal   (Followers: 3, SJR: 0.627, h-index: 14)
Agricultural and Forest Entomology     Hybrid Journal   (Followers: 16, SJR: 0.925, h-index: 43)
Agricultural Economics     Hybrid Journal   (Followers: 45, SJR: 1.099, h-index: 51)
AIChE J.     Hybrid Journal   (Followers: 32, SJR: 1.122, h-index: 120)
Alcoholism and Drug Abuse Weekly     Hybrid Journal   (Followers: 7)
Alcoholism Clinical and Experimental Research     Hybrid Journal   (Followers: 7, SJR: 1.416, h-index: 125)
Alimentary Pharmacology & Therapeutics     Hybrid Journal   (Followers: 34, SJR: 2.833, h-index: 138)
Alimentary Pharmacology & Therapeutics Symposium Series     Hybrid Journal   (Followers: 3)
Allergy     Hybrid Journal   (Followers: 51, SJR: 3.048, h-index: 129)
Alternatives to the High Cost of Litigation     Hybrid Journal   (Followers: 3)
American Anthropologist     Hybrid Journal   (Followers: 152, SJR: 0.951, h-index: 61)
American Business Law J.     Hybrid Journal   (Followers: 24, SJR: 0.205, h-index: 17)
American Ethnologist     Hybrid Journal   (Followers: 93, SJR: 2.325, h-index: 51)
American J. of Economics and Sociology     Hybrid Journal   (Followers: 29, SJR: 0.211, h-index: 26)
American J. of Hematology     Hybrid Journal   (Followers: 35, SJR: 1.761, h-index: 77)
American J. of Human Biology     Hybrid Journal   (Followers: 13, SJR: 1.018, h-index: 58)
American J. of Industrial Medicine     Hybrid Journal   (Followers: 16, SJR: 0.993, h-index: 85)
American J. of Medical Genetics Part A     Hybrid Journal   (Followers: 17, SJR: 1.115, h-index: 61)
American J. of Medical Genetics Part B: Neuropsychiatric Genetics     Hybrid Journal   (Followers: 4, SJR: 1.771, h-index: 107)
American J. of Medical Genetics Part C: Seminars in Medical Genetics     Partially Free   (Followers: 6, SJR: 2.315, h-index: 79)
American J. of Physical Anthropology     Hybrid Journal   (Followers: 38, SJR: 1.41, h-index: 88)
American J. of Political Science     Hybrid Journal   (Followers: 296, SJR: 5.101, h-index: 114)
American J. of Primatology     Hybrid Journal   (Followers: 15, SJR: 1.197, h-index: 63)
American J. of Reproductive Immunology     Hybrid Journal   (Followers: 4, SJR: 1.347, h-index: 75)
American J. of Transplantation     Hybrid Journal   (Followers: 18, SJR: 2.792, h-index: 140)
American J. on Addictions     Hybrid Journal   (Followers: 10, SJR: 0.843, h-index: 57)
Anaesthesia     Hybrid Journal   (Followers: 141, SJR: 1.404, h-index: 88)
Analyses of Social Issues and Public Policy     Hybrid Journal   (Followers: 10, SJR: 0.397, h-index: 18)
Analytic Philosophy     Hybrid Journal   (Followers: 20)
Anatomia, Histologia, Embryologia: J. of Veterinary Medicine Series C     Hybrid Journal   (Followers: 3, SJR: 0.295, h-index: 27)
Anatomical Sciences Education     Hybrid Journal   (Followers: 1, SJR: 0.633, h-index: 24)
Andrologia     Hybrid Journal   (Followers: 2, SJR: 0.528, h-index: 45)
Andrology     Hybrid Journal   (Followers: 2, SJR: 0.979, h-index: 14)
Angewandte Chemie     Hybrid Journal   (Followers: 166)
Angewandte Chemie Intl. Edition     Hybrid Journal   (Followers: 234, SJR: 6.229, h-index: 397)
Animal Conservation     Hybrid Journal   (Followers: 41, SJR: 1.576, h-index: 62)
Animal Genetics     Hybrid Journal   (Followers: 8, SJR: 0.957, h-index: 67)
Animal Science J.     Hybrid Journal   (Followers: 6, SJR: 0.569, h-index: 24)
Annalen der Physik     Hybrid Journal   (Followers: 5, SJR: 1.46, h-index: 40)
Annals of Anthropological Practice     Partially Free   (Followers: 2, SJR: 0.187, h-index: 5)
Annals of Applied Biology     Hybrid Journal   (Followers: 7, SJR: 0.816, h-index: 56)
Annals of Clinical and Translational Neurology     Open Access   (Followers: 1)
Annals of Human Genetics     Hybrid Journal   (Followers: 9, SJR: 1.191, h-index: 67)
Annals of Neurology     Hybrid Journal   (Followers: 49, SJR: 5.584, h-index: 241)
Annals of Noninvasive Electrocardiology     Hybrid Journal   (Followers: 1, SJR: 0.531, h-index: 38)
Annals of Public and Cooperative Economics     Hybrid Journal   (Followers: 8, SJR: 0.336, h-index: 23)
Annals of the New York Academy of Sciences     Hybrid Journal   (Followers: 5, SJR: 2.389, h-index: 189)
Annual Bulletin of Historical Literature     Hybrid Journal   (Followers: 12)
Annual Review of Information Science and Technology     Hybrid Journal   (Followers: 14)
Anthropology & Education Quarterly     Hybrid Journal   (Followers: 26, SJR: 0.72, h-index: 31)
Anthropology & Humanism     Hybrid Journal   (Followers: 18, SJR: 0.137, h-index: 3)
Anthropology News     Hybrid Journal   (Followers: 15)
Anthropology of Consciousness     Hybrid Journal   (Followers: 11, SJR: 0.172, h-index: 5)
Anthropology of Work Review     Hybrid Journal   (Followers: 11, SJR: 0.256, h-index: 5)
Anthropology Today     Hybrid Journal   (Followers: 93, SJR: 0.545, h-index: 15)
Antipode     Hybrid Journal   (Followers: 52, SJR: 2.212, h-index: 69)
Anz J. of Surgery     Hybrid Journal   (Followers: 8, SJR: 0.432, h-index: 59)
Anzeiger für Schädlingskunde     Hybrid Journal   (Followers: 1)
Apmis     Hybrid Journal   (Followers: 1, SJR: 0.855, h-index: 73)
Applied Cognitive Psychology     Hybrid Journal   (Followers: 73, SJR: 0.754, h-index: 69)
Applied Organometallic Chemistry     Hybrid Journal   (Followers: 7, SJR: 0.632, h-index: 58)
Applied Psychology     Hybrid Journal   (Followers: 181, SJR: 1.023, h-index: 64)
Applied Psychology: Health and Well-Being     Hybrid Journal   (Followers: 51, SJR: 0.868, h-index: 13)
Applied Stochastic Models in Business and Industry     Hybrid Journal   (Followers: 5, SJR: 0.613, h-index: 24)
Aquaculture Nutrition     Hybrid Journal   (Followers: 14, SJR: 1.025, h-index: 55)
Aquaculture Research     Hybrid Journal   (Followers: 32, SJR: 0.807, h-index: 60)
Aquatic Conservation Marine and Freshwater Ecosystems     Hybrid Journal   (Followers: 36, SJR: 1.047, h-index: 57)
Arabian Archaeology and Epigraphy     Hybrid Journal   (Followers: 11, SJR: 0.453, h-index: 11)
Archaeological Prospection     Hybrid Journal   (Followers: 12, SJR: 0.922, h-index: 21)
Archaeology in Oceania     Hybrid Journal   (Followers: 13, SJR: 0.745, h-index: 18)
Archaeometry     Hybrid Journal   (Followers: 30, SJR: 0.809, h-index: 48)
Archeological Papers of The American Anthropological Association     Hybrid Journal   (Followers: 15, SJR: 0.156, h-index: 2)
Architectural Design     Hybrid Journal   (Followers: 27, SJR: 0.261, h-index: 9)
Archiv der Pharmazie     Hybrid Journal   (Followers: 3, SJR: 0.628, h-index: 43)
Archives of Drug Information     Hybrid Journal   (Followers: 5)
Archives of Insect Biochemistry and Physiology     Hybrid Journal   (SJR: 0.768, h-index: 54)
Area     Hybrid Journal   (Followers: 13, SJR: 0.938, h-index: 57)
Art History     Hybrid Journal   (Followers: 267, SJR: 0.153, h-index: 13)
Arthritis & Rheumatology     Hybrid Journal   (Followers: 55, SJR: 1.984, h-index: 20)
Arthritis Care & Research     Hybrid Journal   (Followers: 27, SJR: 2.256, h-index: 114)
Artificial Organs     Hybrid Journal   (Followers: 1, SJR: 0.872, h-index: 60)
ASHE Higher Education Reports     Hybrid Journal   (Followers: 15)
Asia & the Pacific Policy Studies     Open Access   (Followers: 16)
Asia Pacific J. of Human Resources     Hybrid Journal   (Followers: 326, SJR: 0.494, h-index: 19)
Asia Pacific Viewpoint     Hybrid Journal   (Followers: 1, SJR: 0.616, h-index: 26)
Asia-Pacific J. of Chemical Engineering     Hybrid Journal   (Followers: 8, SJR: 0.345, h-index: 20)
Asia-pacific J. of Clinical Oncology     Hybrid Journal   (Followers: 6, SJR: 0.554, h-index: 14)
Asia-Pacific J. of Financial Studies     Hybrid Journal   (SJR: 0.241, h-index: 7)
Asia-Pacific Psychiatry     Hybrid Journal   (Followers: 4, SJR: 0.377, h-index: 7)
Asian Economic J.     Hybrid Journal   (Followers: 8, SJR: 0.234, h-index: 21)
Asian Economic Policy Review     Hybrid Journal   (Followers: 4, SJR: 0.196, h-index: 12)
Asian J. of Control     Hybrid Journal   (SJR: 0.862, h-index: 34)
Asian J. of Endoscopic Surgery     Hybrid Journal   (Followers: 1, SJR: 0.394, h-index: 7)
Asian J. of Organic Chemistry     Hybrid Journal   (Followers: 6, SJR: 1.443, h-index: 19)
Asian J. of Social Psychology     Hybrid Journal   (Followers: 5, SJR: 0.665, h-index: 37)
Asian Politics and Policy     Hybrid Journal   (Followers: 12, SJR: 0.207, h-index: 7)
Asian Social Work and Policy Review     Hybrid Journal   (Followers: 5, SJR: 0.318, h-index: 5)
Asian-pacific Economic Literature     Hybrid Journal   (Followers: 5, SJR: 0.168, h-index: 15)
Assessment Update     Hybrid Journal   (Followers: 4)
Astronomische Nachrichten     Hybrid Journal   (Followers: 3, SJR: 0.701, h-index: 40)
Atmospheric Science Letters     Open Access   (Followers: 29, SJR: 1.332, h-index: 27)
Austral Ecology     Hybrid Journal   (Followers: 15, SJR: 1.095, h-index: 66)
Austral Entomology     Hybrid Journal   (Followers: 9, SJR: 0.524, h-index: 28)
Australasian J. of Dermatology     Hybrid Journal   (Followers: 8, SJR: 0.714, h-index: 40)
Australasian J. On Ageing     Hybrid Journal   (Followers: 6, SJR: 0.39, h-index: 22)
Australian & New Zealand J. of Statistics     Hybrid Journal   (Followers: 14, SJR: 0.275, h-index: 28)
Australian Accounting Review     Hybrid Journal   (Followers: 3, SJR: 0.709, h-index: 14)
Australian and New Zealand J. of Family Therapy (ANZJFT)     Hybrid Journal   (Followers: 3, SJR: 0.382, h-index: 12)
Australian and New Zealand J. of Obstetrics and Gynaecology     Hybrid Journal   (Followers: 47, SJR: 0.814, h-index: 49)
Australian and New Zealand J. of Public Health     Hybrid Journal   (Followers: 13, SJR: 0.82, h-index: 62)
Australian Dental J.     Hybrid Journal   (Followers: 6, SJR: 0.482, h-index: 46)
Australian Economic History Review     Hybrid Journal   (Followers: 6, SJR: 0.171, h-index: 12)
Australian Economic Papers     Hybrid Journal   (Followers: 31, SJR: 0.23, h-index: 9)
Australian Economic Review     Hybrid Journal   (Followers: 6, SJR: 0.357, h-index: 21)
Australian Endodontic J.     Hybrid Journal   (Followers: 3, SJR: 0.513, h-index: 24)
Australian J. of Agricultural and Resource Economics     Hybrid Journal   (Followers: 3, SJR: 0.765, h-index: 36)
Australian J. of Grape and Wine Research     Hybrid Journal   (Followers: 5, SJR: 0.879, h-index: 56)
Australian J. of Politics & History     Hybrid Journal   (Followers: 15, SJR: 0.203, h-index: 14)
Australian J. of Psychology     Hybrid Journal   (Followers: 18, SJR: 0.384, h-index: 30)
Australian J. of Public Administration     Hybrid Journal   (Followers: 429, SJR: 0.418, h-index: 29)
Australian J. of Rural Health     Hybrid Journal   (Followers: 6, SJR: 0.43, h-index: 34)
Australian Occupational Therapy J.     Hybrid Journal   (Followers: 74, SJR: 0.59, h-index: 29)
Australian Psychologist     Hybrid Journal   (Followers: 12, SJR: 0.331, h-index: 31)
Australian Veterinary J.     Hybrid Journal   (Followers: 23, SJR: 0.459, h-index: 45)
Autism Research     Hybrid Journal   (Followers: 37, SJR: 2.126, h-index: 39)
Autonomic & Autacoid Pharmacology     Hybrid Journal   (SJR: 0.371, h-index: 29)
Banks in Insurance Report     Hybrid Journal   (Followers: 1)
Basic & Clinical Pharmacology & Toxicology     Hybrid Journal   (Followers: 11, SJR: 0.539, h-index: 70)
Basic and Applied Pathology     Open Access   (Followers: 2, SJR: 0.113, h-index: 4)
Basin Research     Hybrid Journal   (Followers: 5, SJR: 1.54, h-index: 60)
Bauphysik     Hybrid Journal   (Followers: 2, SJR: 0.194, h-index: 5)
Bauregelliste A, Bauregelliste B Und Liste C     Hybrid Journal  
Bautechnik     Hybrid Journal   (Followers: 1, SJR: 0.321, h-index: 11)
Behavioral Interventions     Hybrid Journal   (Followers: 9, SJR: 0.297, h-index: 23)
Behavioral Sciences & the Law     Hybrid Journal   (Followers: 23, SJR: 0.736, h-index: 57)
Berichte Zur Wissenschaftsgeschichte     Hybrid Journal   (Followers: 10, SJR: 0.11, h-index: 5)
Beton- und Stahlbetonbau     Hybrid Journal   (Followers: 2, SJR: 0.493, h-index: 14)
Biochemistry and Molecular Biology Education     Hybrid Journal   (Followers: 6, SJR: 0.311, h-index: 26)
Bioelectromagnetics     Hybrid Journal   (Followers: 1, SJR: 0.568, h-index: 64)
Bioengineering & Translational Medicine     Open Access  
BioEssays     Hybrid Journal   (Followers: 10, SJR: 3.104, h-index: 155)
Bioethics     Hybrid Journal   (Followers: 14, SJR: 0.686, h-index: 39)
Biofuels, Bioproducts and Biorefining     Hybrid Journal   (Followers: 1, SJR: 1.725, h-index: 56)
Biological J. of the Linnean Society     Hybrid Journal   (Followers: 18, SJR: 1.172, h-index: 90)
Biological Reviews     Hybrid Journal   (Followers: 5, SJR: 6.469, h-index: 114)
Biologie in Unserer Zeit (Biuz)     Hybrid Journal   (Followers: 41, SJR: 0.12, h-index: 1)
Biology of the Cell     Full-text available via subscription   (Followers: 9, SJR: 1.812, h-index: 69)
Biomedical Chromatography     Hybrid Journal   (Followers: 6, SJR: 0.572, h-index: 49)
Biometrical J.     Hybrid Journal   (Followers: 5, SJR: 0.784, h-index: 44)
Biometrics     Hybrid Journal   (Followers: 37, SJR: 1.906, h-index: 96)
Biopharmaceutics and Drug Disposition     Hybrid Journal   (Followers: 10, SJR: 0.715, h-index: 44)
Biopolymers     Hybrid Journal   (Followers: 18, SJR: 1.199, h-index: 104)
Biotechnology and Applied Biochemistry     Hybrid Journal   (Followers: 44, SJR: 0.415, h-index: 55)
Biotechnology and Bioengineering     Hybrid Journal   (Followers: 157, SJR: 1.633, h-index: 146)
Biotechnology J.     Hybrid Journal   (Followers: 14, SJR: 1.185, h-index: 51)
Biotechnology Progress     Hybrid Journal   (Followers: 39, SJR: 0.736, h-index: 101)
Biotropica     Hybrid Journal   (Followers: 20, SJR: 1.374, h-index: 71)
Bipolar Disorders     Hybrid Journal   (Followers: 9, SJR: 2.592, h-index: 100)
Birth     Hybrid Journal   (Followers: 38, SJR: 0.763, h-index: 64)
Birth Defects Research Part A : Clinical and Molecular Teratology     Hybrid Journal   (Followers: 2, SJR: 0.727, h-index: 77)
Birth Defects Research Part B: Developmental and Reproductive Toxicology     Hybrid Journal   (Followers: 7, SJR: 0.468, h-index: 47)
Birth Defects Research Part C : Embryo Today : Reviews     Hybrid Journal   (SJR: 1.513, h-index: 55)
BJOG : An Intl. J. of Obstetrics and Gynaecology     Partially Free   (Followers: 248, SJR: 2.083, h-index: 125)

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Journal Cover Animal Science Journal
  [SJR: 0.569]   [H-I: 24]   [6 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 1344-3941 - ISSN (Online) 1740-0929
   Published by John Wiley and Sons Homepage  [1592 journals]
  • Analysis of influencing factors of boar claw lesion and lameness
    • Authors: Chao Wang; Jia-Lian Li, Hong-Kui Wei, Yuan-Fei Zhou, Jia-Jian Tan, Hai-Qing Sun, Si-Wen Jiang, Jian Peng
      Abstract: This study aimed to investigate the factors affecting boar claw lesions and lameness. A total of 1299 boars were examined for claw lesions and lameness, including 788 boars reared in individual pens with solid concreted floor (IPS) and 511 boars raised in individual stalls with slatted floor (ISS). Flooring type showed significant impacts on all claw lesion types (P 
      PubDate: 2018-02-06T03:05:58.756141-05:
      DOI: 10.1111/asj.12974
  • Multi-level mixed models for evaluating factors affecting the mortality
           and weaning weight of piglets in large-scale commercial farms in central
    • Authors: Ze-Xue Liu; Hong-Kui Wei, Yuan-Fei Zhou, Jian Peng
      Abstract: This study investigated the factors affecting piglet mortality (square root of mortality, SQRM) and average weaning weight (AWW) in commercial farms in central China. Information on sow diets, management and climate from 2478 weaning batches completed in 16 pig farms was collected from 2009 to 2013. Multi-level mixed models, which included batch level (level 1) and farm level (level 2), were used to analyze the factors associated with SQRM and AWW. The mean values of SQRM and AWW were 2.52% (SD = 0.96) and 7.31 (SD = 0.77), respectively. Lactation sow diets supplemented with oregano essential oils (OEOs) decreased the SQRM (P 
      PubDate: 2018-02-04T22:55:21.784012-05:
      DOI: 10.1111/asj.12963
  • Genetic diversity and population structure of Polish Arabian horses
           assessed through breeding and microsatellite data
    • Authors: Iwona Głażewska; Barbara Gralak, Aleksandra M. Naczk, Beata Prusak
      Abstract: Polish Arabian horses are one of the most important populations of this breed in the world. Their post-war history can be divided into two periods, with the dominant role of state studs until 1989, and the increasing significance of private breeding in the next years. The goal of the study was to evaluate genetic diversity and structure of the population under a new breeding policy. The analyses of breeding and microsatellite data from 1996 to 2012 provide a coherent picture of the population with constant flow of horses only in one direction from state to private studs. An increase in the number of broodmares was observed, from 396 mares in 1996 to 1021 mares in 2012. The proportion of foreign sires used in Polish studs also increased, from 7.1% to 37.0%. An increasing number of alleles and progressive differentiation in mares were observed. STRUCTURE analysis indicated that the Polish horses were clearly separated from foreign horses used in Polish breeding, although only one of the 75 alleles found can be considered as typically Polish. The high heterozygosity is an important feature of the Polish population; however, the decrease of heterozygosity in state broodmares was noted. This issue needs to be studied further.
      PubDate: 2018-02-02T04:05:44.046959-05:
      DOI: 10.1111/asj.12983
  • Hypolipidemic and anti-inflammatory effects of aorta and heart tissues of
           cattle and pigs in the atherosclerosis rat model
    • Authors: Irina M. Chernukha; Liliya V. Fedulova, Elena A. Kotenkova, Shiro Takeda, Ryoichi Sakata
      Abstract: The aim of this study was to investigate the effects of aorta and heart tissues obtained from cattle and pigs on atherosclerosis disorders. Atherosclerosis model rats were provided with the respective diets consisting of aorta and heart tissues. Administration of each tissue suppressed body weight gain as compared to that of the control. In particular, the aorta tissues of pigs and cattle demonstrated significant suppressions in body weight gain in the model rats. The aorta tissues of pigs and cattle showed a significant increase and decrease in the serum high-density lipoproteins and atherogenic index, respectively, which was correlated with the increase in apolipoprotein A1. Hematological analysis revealed that aorta tissues of pigs and cattle clearly reduced the ratio of granulocytes/lymphocytes in the atherosclerosis rats. Serum vascular cellular adhesion molecule-1 levels in the atherosclerosis rats, which were administered these aorta tissues, were also significantly reduced. Additionally, there was an increase in von Willebrand factor in the rat serum. Based on the results obtained, the aorta tissues of pigs and cattle, in particular, demonstrated positive effects in the atherosclerosis rats due to the alteration of lipid metabolism and reduction in inflammation related to atherosclerosis.
      PubDate: 2018-02-01T20:00:24.03954-05:0
      DOI: 10.1111/asj.12986
  • A genome-wide association study for fat-related traits computed by image
           analysis in Japanese Black cattle
    • Authors: Ayaka Nakajima; Fuki Kawaguchi, Yoshinobu Uemoto, Moriyuki Fukushima, Emi Yoshida, Eiji Iwamoto, Takayuki Akiyama, Namiko Kohama, Eiji Kobayashi, Takeshi Honda, Kenji Oyama, Hideyuki Mannen, Shinji Sasazaki
      Abstract: The objective of this study was to identify genomic regions associated with fat-related traits using a Japanese Black cattle population in Hyogo. From 1836 animals, those with high or low values were selected on the basis of corrected phenotype and then pooled into high and low groups (n = 100 each), respectively. DNA pool-based genome-wide association study (GWAS) was performed using Illumina BovineSNP50 BeadChip v2 with three replicate assays for each pooled sample. GWAS detected that two single nucleotide polymorphisms (SNPs) on BTA7 (ARS-BFGL-NGS-35463 and Hapmap23838-BTA-163815) and one SNP on BTA12 (ARS-BFGL-NGS-2915) significantly affected fat percentage (FAR). The significance of ARS-BFGL-NGS-35463 on BTA7 was confirmed by individual genotyping in all pooled samples. Moreover, association analysis between SNP and FAR in 803 Japanese Black cattle revealed a significant effect of SNP on FAR. Thus, further investigation of these regions is required to identify FAR-associated genes and mutations, which can lead to the development of DNA markers for marker-assisted selection for the genetic improvement of beef quality.
      PubDate: 2018-01-30T03:45:28.978698-05:
      DOI: 10.1111/asj.12987
  • Effects of intravenous tryptophan infusion on thermoregulation in steers
           exposed to acute heat stress
    • Authors: Madoka Sutoh; Etsuko Kasuya, Ken-ichi Yayou
      Abstract: This study was conducted to investigate the effect of tryptophan (TRP) supply on the thermoregulatory responses via brain serotonin (5-HT) in cattle. In period 1, 12 Holstein steers were kept under a constant room temperature (22°C) and were administered the intravenous (i.v.) infusion of saline or TRP (38.5 mg/kg/2 h). Changes in rectal temperature (RT), 5-HT concentration in the cerebrospinal fluid (CSF), and other factors involved in thermoregulation were measured. In period 2, the steers received the same treatments as in period 1; however, the room temperature was elevated from 22°C to 33°C during i.v. infusion and maintained at 33°C for 3 h. 5-HT concentration in CSF increased following TRP infusion in both periods, and RT significantly decreased following TRP infusion only in period 2. The effect of TRP on respiration rate and plasma prolactin and total triiodothyronine concentrations was not significant. These results suggest that increase in TRP supply can attenuate increase in RT in response to acute heat stress through the increase in brain 5-HT, followed by presumable increase in evaporative heat loss from the skin surface in cattle. It is possible that the increase in peripheral blood TRP metabolites could also participate in the hypothermic effect of TRP.
      PubDate: 2018-01-19T01:10:41.234523-05:
      DOI: 10.1111/asj.12988
  • Research on the preparation, uniformity and stability of mixed standard
           substance for rapid detection of goat milk composition
    • Authors: Yuying Zhu; Jianmin Wang, Cunfang Wang
      Abstract: Taking fresh goat milk as raw material after filtering, centrifuging, hollow fiber ultrafiltration, allocating formula, value detection and preparation processing, a set of 10 goat milk mixed standard substances was prepared on the basis of one-factor-at-a-time using a uniform design method, and its accuracy, uniformity and stability were evaluated by paired t-test and F-test of one-way analysis of variance. The results showed that three milk composition contents of these standard products were independent of each other, and the preparation using the quasi-level design method, and without emulsifier was the best program. Compared with detection value by cow milk standards for calibration fast analyzer, the calibration by goat milk mixed standard was more applicable to rapid detection of goat milk composition, detection value was more accurate and the deviation showed less error. Single factor analysis of variance showed that the uniformity and stability of the mixed standard substance were better; it could be stored for 15 days at 4°C. The uniformity and stability of the in-units and inter-units could meet the requirements of the preparation of national standard products.
      PubDate: 2018-01-19T01:00:34.201916-05:
      DOI: 10.1111/asj.12985
  • Effects of traditional Chinese medicine formula on ruminal fermentation,
           enzyme activities and nutrient digestibility of beef cattle
    • Authors: Zhi Zhu; Zhen-hui Song, Li-ting Cao, Yong Wang, Wen-zhang Zhou, Pei Zhou, Fu-yuan Zuo
      Abstract: This study was conducted to evaluate effects of traditional Chinese medicine formula (TCMF) combined with several herbs on ruminal fermentation, enzyme activities and nutrient digestibility. Twenty finishing bulls were assigned to control or different TCMFs (Yufeisan-1, -2, -3; 2.5% dry matter (DM) in concentrate). Results showed that DM intake was higher (P 
      PubDate: 2018-01-12T04:15:23.173503-05:
      DOI: 10.1111/asj.12978
  • Effects of different rearing systems on growth performance, carcass
           traits, meat quality and serum biochemical parameters of Chaohu ducks
    • Authors: Cheng Zhang; Richard-Hermann Ah Kan Razafindrabe, Kaikai Chen, Xiaohui Zhao, Lei Yang, Li Wang, Xingyong Chen, Sihua Jin, Zhaoyu Geng
      Abstract: This study was conducted using a total of 360 22-day-old Chaohu ducks to evaluate the effect of rearing system on growth performance, carcass traits, meat quality and serum parameters of male and female Chaohu ducks. The birds were divided and raised in separate pens according to sex and rearing system, with three replicate pens of 30 male or 30 female ducks per pen for each rearing system. The rearing systems consisted of a floor rearing system (FRS) and a net rearing system (NRS). Results showed that ducks raised in NRS had better growth performance, whereas, ducks raised in FRS exhibited better carcass traits and meat color, and lower intramuscular fat. For the serum parameters, NRS significantly decreased high-density lipoprotein cholesterol content, and enhanced total protein and triacylglycerol contents. Male ducks had lower abdominal fat percentage, and higher growth performance and shear force, but there were no other significant differences between sexes. No rearing system × sex interaction was observed in the present study, revealing that rearing system had the same effect on both sexes. In conclusion, NRS was beneficial to the growth performance of Chaohu ducks, whereas this system had some negative effects on carcass traits, meat quality and serum profiles.
      PubDate: 2018-01-09T22:11:04.91653-05:0
      DOI: 10.1111/asj.12976
  • Evaluation of three hormonal protocols for anovulatory lactating cows
           under regulations restricting the use of estrogenic compounds
    • Authors: Guang-Min Yu; Yue Wu, Xian-Long Wang, Shuan Zhao, Teruo Maeda, Shen-Ming Zeng
      Abstract: When European Union regulations restricted the use of estrogenic compounds in food-producing animals, refined hormonal protocols were no longer applicable for anovulatory cows. However, Ovsynch and its adaptations are routinely and uniformly applied to all cows regardless of ovarian function. To evaluate their efficacy on anovulatory cows, 143, 147 and 144 anovulatory cows received Ovsynch, Presynch and G6G protocols, respectively. In comparison, 150 cyclic cows were bred without using a synchronized protocol. Results showed that cows in the Presynch group had luteolysis responding to the last prostaglandin F2α (PGF2α) injection greater than the Ovsynch group. The serous progesterone levels at the first gonadotropin-releasing hormone of Ovsych and the last PGF2α injection was greater in the G6G group than the other two hormonal treatment groups. Concentrations of Ca2+ and total protein in cervical mucus in all three hormone-treated groups before artificial insemination (AI) were significantly different from the controls. The G6G group obtained a greater pregnancy rate compared with Ovsynch and Presynch, but significantly less than the controls. For open cows in the Ovsynch group, estrus rate within 24 days after the first AI was significantly less than the controls. In conclusion, the G6G treatment resulted to better reproductive performance in anovulatory cows.
      PubDate: 2018-01-09T21:50:51.921671-05:
      DOI: 10.1111/asj.12953
  • The effect of transport on the quality of rabbit meat
    • Authors: Joanna Składanowska-Baryza; Agnieszka Ludwiczak, Ewa Pruszyńska-Oszmałek, Paweł Kołodziejski, Marta Bykowska, Marek Stanisz
      Abstract: The analyzed material included 40 hybrid rabbits slaughtered at the age of 90 days. The control group was transported directly after weaning, while the transport group was transported directly prior to slaughter. The experiment was designed to assess the transport stress, carcass and meat quality implications, taking into account the muscle type and sex. The transported animals were characterized by a higher level of blood cortisol, glucose and triglycerides (P 
      PubDate: 2018-01-08T21:31:04.978847-05:
      DOI: 10.1111/asj.12966
  • Effects of an immune stimulant, inactivated Mycobacterium phlei, on the
           growth performance as well as meat quality of fattening pigs
    • Authors: Jin-Feng Zhong; Wei-Gao Wu, Aseyinjan Tastan, Xiao-Qing Zhang, Bin Wang, Xiao-Peng Tang, Re-Jun Fang
      Abstract: Inactivated mycobacterium phlei (M. phlei) is well known for its immune-stimulatory functions in humans and livestock, but less information is available about the influence on meat quality of pigs when used as a feed additive. This study was designed to evaluate the effects of inactivated M. phlei on growth performance as well as meat quality of fattening pigs. A total of 240 cross-bred pigs ([Landrace × Yorkshire] × Duroc) with initial body weight of 80.14 ± 0.29 kg were randomly allocated to five treatments, each of which consisted of eight replicates with 6six pigs per replicate. The basal diet supplemented with five levels of inactivated M. phlei preparations (0, 3.5 × 109 [0.1% w/w], 7 × 109 [0.2%], 1.4 × 1010 [0.4%] or 2.1 × 1010 [0.6%] colony-forming units/kg) was respectively fed to the control group and four treatment groups for 30 days. Adding 0.4% of inactivated M. phlei to diet increased the average daily feed intake and average daily gain of pigs. Importantly, intramuscular fat percentage in the Longissimus dorsi (LD) was increased by feeding diet containing 0.2%, 0.4% and 0.6% of inactivated M. phlei, despite the pH value, drip loss, cooking loss and filter paper fluid uptake not being influenced. Analysis of the fatty acid components showed that some saturated fatty acids were decreased in LD after feeding inactivated M. phlei, but some monounsaturated fat acids (MUFAs) and polyunsaturated fatty acids were increased (PUFAs), which induced the total contents of MUFAs and PUFAs were improved. RT-PCR assay revealed that feeding inactivated M. phlei up-regulated genes implicated in fat metabolism in muscle, including ELOVL6, FASN, SCD1 and H-FABP. This study revealed that feeding inactivated M. phlei not only increased growth performance of fattening pigs, but also improved the meat quality by increasing intramuscular fat content, thus inactivated M. phlei probably has high utilization value in modern pig production.
      PubDate: 2018-01-03T07:39:12.549791-05:
      DOI: 10.1111/asj.12967
  • Issue Information
    • Pages: 271 - 272
      PubDate: 2018-02-01T19:37:48.756096-05:
      DOI: 10.1111/asj.12902
  • Estimation of dietary threonine requirement using plasma amino acid
           concentrations in mature thoroughbreds
    • Authors: Tatsuyuki Yoshida; Yoshiyuki Ohta
      Abstract: The experiment was conducted to estimate dietary threonine (Thr) requirement using plasma amino acid concentrations as a criterion in mature thoroughbreds. Four adult thoroughbreds were used, and a 4 × 4 Latin square design was used for four dietary Thr levels. Plasma Thr concentration was constant until 0.41%, and then increased rapidly with increasing dietary Thr levels. The Thr requirement was estimated to be 67% of lysine with plasma Thr concentration at four Thr levels.
      PubDate: 2017-12-28T23:45:21.793152-05:
      DOI: 10.1111/asj.12975
  • Effect of FH535 on in vitro maturation of porcine oocytes by
           inhibiting WNT signaling pathway
    • Authors: Meihong Shi; Jianyong Cheng, Yamei He, Zhongliang Jiang, Bello M. Bodinga, Boyang Liu, Huali Chen, Qingwang Li
      Abstract: Wingless-int (WNT) signaling pathway is vital to modulate life processes, including cell fate determination, cell differentiation, cell proliferation, cell apoptosis and embryogenesis. To demonstrate the uncertain effect of the canonical WNT signaling pathway on oocyte maturation, immature porcine oocytes were collected and cultured in vitro with the WNT/β-catenin inhibitor FH535. The concentrations of FH535 were selected as 0.00, 0.01, 0.10, 1.00 and 10.00 μmol/L. The results showed that the optimum concentration of FH535 on oocyte maturation was 1.00 μmol/L. In this concentration, the proportion of MII oocytes increased (P  0.05), while the rate of blastocysts in the 1.00 μmol/L FH535 treated group was higher than that of control (P 
      PubDate: 2017-12-28T19:56:16.908253-05:
      DOI: 10.1111/asj.12982
  • Inclusion of Bacillus amyloliquefaciens strain TOA5001 in the diet of
           broilers suppresses the symptoms of coccidiosis by modulating intestinal
    • Authors: Takamitsu Tsukahara; Ryo Inoue, Keizo Nakayama, Takio Inatomi
      Abstract: Coccidiosis is an intestinal parasitic infection and one of the most prevalent and economically damaging diseases of chickens. Furthermore, coccidia-induced mucogenesis promotes secondary colonization by Clostridium perfringens, a major pathogen of chickens that causes necrotic enteritis. Our previous work found that supernatant of a culture of Bacillus amyloliquefaciens strain TOA5001 (BA) inhibited the growth of C. perfringens on Gifu anaerobic broth medium. Accordingly, we evaluated the effectiveness of dietary BA administration in inhibiting C. perfringens colonization of the intestine in broilers that were experimentally infected with coccidia. Ten healthy broilers from a BA-supplemented (2 × 105 colony-forming units/g of feed) broiler group and 10 from a non-treated group were challenged with Eimeria tenella and E. maxima (5000 oocysts of each species/chick) at 28 days old. At 36 days old, five chicks from each group were slaughtered, whereas the remaining five in each group were killed at 49 days old. Dietary BA administration into Eimeria-challenged birds reduced coccidial symptoms such as intestinal lesions. It also modified the cecal microbiota through suppressing C. perfringens and E. coli colonization, and inducing domination of Faecalibacterium prausnitzii, the Lactobacillus group and unknown Lachnospiraceae genera by bacterial DNA-based metagenome analyses. B. amyloliquefaciens TOA5001 supplementation suppressed the symptoms of coccidiosis by modulating cecal microbiota in Eimeria-challenged broilers.
      PubDate: 2017-12-28T02:40:30.630202-05:
      DOI: 10.1111/asj.12980
  • Gene expression profiling in embryonic chicken ovary during asymmetric
    • Authors: Amir Hossan Shaikat; Shoko Namekawa, Sadequllah Ahmadi, Misa Takeda, Takeshi Ohkubo
      Abstract: The reproductive system in female birds arises as bilateral asymmetrical anlagen, excluding the birds of prey. Earlier, histological and messenger RNA (mRNA) expression profile studies of several genes related to gonadal sex differentiation in chicken embryos tried to elucidate the query of this asymmetry in a scattered manner. To understand the matter precisely, we have focused on mRNA expression of a cohort of genes (FSHR, CYP19A1, caspase 3, caspase 8) in second half of the embryonic days (E10–E18). The established role of leptin in development of the embryo and its expression in the embryonic ovary also drove us to check leptin receptor (LEPR) expression in the ovary. Increased expression of FSHR and CYP19A1 in the left ovary compared with that in the right ovary was identified (P 
      PubDate: 2017-12-28T02:30:30.891431-05:
      DOI: 10.1111/asj.12979
  • Effects of different gestation housing types on reproductive performance
           of sows
    • Authors: Jeehwan Choe; Sheena Kim, Jin Ho Cho, Jeong Jae Lee, Sangwoo Park, Byeonghyeon Kim, Junsu Kim, Samuel K. Baidoo, Sangnam Oh, Hyeun Bum Kim, Minho Song
      Abstract: This study evaluated the effects of different gestation housing types on reproductive performance of sows. A total of 60 sows (218 ± 24 kg body weight) with mixed parity were used. During gestation, 28 sows were housed in groups with electronic sow feeders (space allowance = 1.26 m2/sow) and 32 sows were housed in individual stalls (space allowance = 1.20 m2/sow). Sows from both housing types were moved to farrowing crates on day 109 of gestation and stayed until weaning (18 days post-farrowing). Typical corn-soybean meal diets were provided to sows during gestation and lactation. Measurements were reproductive performance of sows at farrowing as well as performance of sows and their litter during lactation. Similar total numbers of piglets born at farrowing were observed for sows gestated in both housing types. However, group-housed gestation sows had more mummies (0.321 vs. 0.064; P 
      PubDate: 2017-12-28T02:17:18.036876-05:
      DOI: 10.1111/asj.12977
  • Effect of chopping time and heating on 1H nuclear magnetic resonance and
           rheological behavior of meat batter matrix
    • Authors: Fen Zhou; Hui Dong, Jun-Hua Shao, Jun-Long Zhang, Deng-Yong Liu
      Abstract: The effect of chopping time and heating on physicochemical properties of meat batters was investigated by low-field nuclear magnetic resonance and rheology technology. Cooking loss and L* increased while texture profile analysis index decreased between chopping 5 and 6 min. The relaxation time T21 (bound water) and its peak area ratio decreased, while the ratio of T22 peak area (immobilized water) in raw meat batters gradually increased with the extension of chopping time. However, T22 was opposite after being heated and a new component T23 (free water) appeared (T2i is the spin – spin relaxation time for the ith component.). The initial damping factor (Tan δ) gradually decreased and there were significant difference between 4 and 5 min of chopping time. There were significantly positive correlations between the ratio of peak area of T22 and chopping time, the storage modulus (G′), cooking loss, and L*, respectively. Continued chopping time could improve the peak area proportion of T22 in raw meat batters. Further, the higher the peak area proportion of T22 in raw meat batters, the cooking loss of heated meat gel was higher. Also, the stronger the mobility of immobilized water in meat batter, the higher the L* of the fresh meat batters. Thus, it is revealed that the physicochemical properties of meat batter are significantly influenced by chopping time which further affects the water holding capacity and the texture of emulsification gel.
      PubDate: 2017-12-27T21:45:49.12033-05:0
      DOI: 10.1111/asj.12971
  • Bacteriological evaluation of composted manure solids prepared from
           anaerobic digested slurry for hygienic recycled bedding materials for
           dairy cows
    • Authors: Eiryu Okamoto; Hiroki Miyanishi, Akinobu Nakamura, Tomoyuki Kobayashi, Norihiko Kobayashi, Yoshinori Terawaki, Hajime Nagahata
      Abstract: Changes in mastitis-causing pathogens, pH and water content in composted manure solids (CMS) prepared from digested slurry were evaluated during turning at 2-day intervals for 8 days (C1–C4). The numbers of streptococci, coagulase-negative staphylococci and coliforms were 2.6 × 101, 1.7 × 102 and 1.0 × 101 colony-forming units (cfu)/g in CMS (C4) (summer), and these counts were markedly lower (P 
      PubDate: 2017-12-27T06:08:11.843383-05:
      DOI: 10.1111/asj.12962
  • Protective effects of melatonin on the in vitro developmental
           competence of bovine oocytes
    • Authors: Yunwei Pang; Shanjiang Zhao, Yeqing Sun, Xiaolong Jiang, Haisheng Hao, Weihua Du, Huabin Zhu
      Abstract: The present study investigated the effects of melatonin on bovine oocyte maturation and subsequent embryonic development in vitro. Results showed that the nuclear and cytoplasmic maturation, characterized by first polar body extrusion, normal distribution of cortical granules and mitochondria, as well as increased mitochondrial membrane potential, were significantly improved in 10−9 mol/L melatonin-treated oocytes. Melatonin supplementation reduced intracellular reactive oxygen species level and enhanced glutathione production. Meanwhile, the presence of melatonin (10−9 mol/L) during oocyte maturation resulted in a decreased early apoptotic rate in oocytes. After in vitro fertilization, oocytes receiving melatonin supplementation exhibited a significantly higher blastocyst formation rate and yielded a markedly lower number of apoptotic cells. Mechanistic explorations showed that addition of 10−9 mol/L melatonin to in vitro maturation media significantly attenuated the transcript level of caspase-3, while the expressions of BCL-2, XIAP, CAT and HSP70 were significantly reinforced in the resultant embryos. Taken together, melatonin ameliorates bovine oocyte maturation potential, and the beneficial effects can affect subsequent embryonic development. The protective role of melatonin may be due to its anti-apoptotic and anti-oxidative activities.
      PubDate: 2017-12-27T05:11:34.528193-05:
      DOI: 10.1111/asj.12970
  • Capsosiphon fulvescens extracts improve obesity-associated metabolic
           disorders and hepatic steatosis in high-fat diet-induced obese mice
    • Authors: Mi-Young Yun; Jae-Sug Lee, Byoung-Soo Kim, Hwa-Jung Choi
      Abstract: Herbal dietary supplements have attracted more and more attention owing to their relative effectiveness in obesity -related metabolic disorders and diseases. This study investigated the therapeutic effects and underlying mechanisms of Capsosiphon fulvescens (CF) extracts on obesity, their associated metabolic disorders and hepatic steatosis in high-fat diet (HFD)-induced obese mice. Male C57BL/6 mice were fed with normal, HFD/Vehicle and HFD/CF (orally 300 mg/kg/day for CF). After 12 weeks, CF blocked HFD-induced body weight, food intake, liver weight, hepatic triglyceride (TG), fat mass (weight of abdominal subcutaneous fat and epididymal adipose tissue) and biochemical parameters (total cohlesterol, glucose, TG, creatinine, high-density lipoproteins cholesterol and low-density lipoprotein cholesterol) of serum. CF also had improved serum levels of adiponectin, leptin and insulin-like growth factor-1 in HFD/CF mice. Moreover, CF ameliorated the hepatic steatosis-reducing size of white adipose tissue. These results indicate that CF have anti-obesity effects and are effective for reducing metabolic risk and hepatic steatosis.
      PubDate: 2017-12-22T06:28:56.366762-05:
      DOI: 10.1111/asj.12969
  • Dietary protein level affects nutrient digestibility and ileal microbiota
           structure in growing pigs
    • Authors: Kai Qiu; Xin Zhang, Ning Jiao, Doudou Xu, Caiyun Huang, Yubo Wang, Jingdong Yin
      Abstract: This study aimed to determine whether dietary protein content influences pig health as indicated by ileal microbiota structure and coefficients of total tract apparent digestibility (CTTAD) of nutrients. Seventy-two gilts, with an initial body weight of 29.9 ± 1.5 kg, were used in this 42-day feeding study. Pigs were randomly allotted to one of three dietary treatments of corn-soybean meal contained 14, 16 or 18% crude protein (CP). As dietary CP content decreased, the CTTAD of most essential amino acids (AAs), except for arginine and histidine, increased linearly, while those of most nonessential AAs decreased linearly. The concentration of total short-chain fatty acids (SCFA) was higher in pigs fed the diet with 14% CP content than others. Ileal microbiota structure was changed by dietary treatments. In particular, at the phylum level, the relative abundance of Tenericutes in ileal digesta decreased as the dietary protein content reduced, while that of cyanobacteria increased. At the genus level, the relative abundance of Weeksella, Phaseolus acutifolius, Slackia, Sulfurimonas and Aerococcus showed significant differences among the three dietary treatments. In conclusion, ileal microbiota structure was changed by dietary protein content. Moderate reduction of protein intake can benefit gut health by enhancing the gut microbial fermentation and SCFA formation.
      PubDate: 2017-12-22T06:16:19.675898-05:
      DOI: 10.1111/asj.12946
  • Effects of complex probiotic supplementation in growing pig diets with and
           without palm kernel expellers on growth performance, nutrient
           digestibility, blood parameters, fecal microbial shedding and noxious gas
    • Authors: Wenchao Liu; Subramaniammohana Devi, Jaewon Park, Inho Kim
      Abstract: In Experiment 1, a total of 100 growing pigs (Duroc × [Landrace × Yorkshire]) with an average initial body weight (BW) of 24.88 ± 1.57 kg were randomly allotted to 2 × 2 factorial arrangement with two concentrations of palm kernel expellers (PKE) in diets at 0% or 10%, and two concentrations of supplemental probiotics at 0 or 6.0 × 107 colony-forming units/kg. There were five replicate pens per treatment with five pigs per pen. In Experiment 2, eight barrows with average initial BW of 25.78 ± 0.19 kg were allotted to a replicated 4 × 4 Latin square design with four diets and four periods per square. Four experimental diets were the same as Experiment 1. In Experiment 1, dietary probiotic supplementation improved (P 
      PubDate: 2017-12-19T02:10:27.116851-05:
      DOI: 10.1111/asj.12965
  • Effect of fructo-oligosaccharides on nutrient digestibility and digesta
           retention time in adult guinea pigs
    • Authors: Kiyonori Kawasaki; Xiao Min, Ei Sakaguchi
      Abstract: A previous study suggested that addition of fructo-oligosaccharides (FOS) to the diet improved nitrogen (N) utilization and decreased acid detergent fiber (ADF) digestibility in guinea pigs. The present study was conducted to clarify the relationship between ADF digestibility and gastrointestinal mean retention time (MRT) in guinea pigs under FOS supplementation. Adult male guinea pigs were fed a commercial diet (50 g/day) with either 5% glucose (glucose group) or 5% FOS (FOS group) for 12 days in individual metabolism cages. Unlike the glucose group, N utilization improved, but ADF digestibility significantly (P 
      PubDate: 2017-12-15T03:45:22.644402-05:
      DOI: 10.1111/asj.12957
  • The effect of administration of copper nanoparticles to chickens in their
           drinking water on the immune and antioxidant status of the blood
    • Authors: Katarzyna Ognik; Iwona Sembratowicz, Ewelina Cholewińska, Jan Jankowski, Krzysztof Kozłowski, Jerzy Juśkiewicz, Zenon Zduńczyk
      Abstract: The aim of this experiment was to determine what dosage of copper (Cu) nanoparticles, added to a standard dietary supplementation with copper sulfate, would improve antioxidant and immune defense in chickens. The experiment was conducted with 126 broiler chickens assigned to seven treatments with three pens per treatment and six broiler chickens per pen. The basal-diet treatment did not receive Cu nanoparticles (nano-Cu) and as shown by analyses it was Cu-deficient (−29% vs. National Research Council (NRC) recommendations; 5.7 vs. 8 mg/kg). Broiler chickens received nano-Cu (0.5, 1.0 or 1.5 mg/kg body weight per day) via a tube into the crop over three 3-day periods (days 8–10, 22–24 and 36–38) or three 7-day periods (days 8–14, 22–28 and 36–42). As a result, in the nano-Cu-treated broilers the total experimental Cu intake was from −11% to +96% versus NRC recommendations. At the age of 42 days of broilers, their blood indices were determined. The obtained results showed that correction of the deficient basal diet of chickens with nano-Cu to a level of copper exceeding the NRC recommendation by 54% increased the antioxidant potential of the organism and inhibited lipid peroxidation. At the dosage of +96% versus NRC, some symptoms of a deterioration in antioxidant status appeared (a decrease in the level of glutathione plus glutathione disulfide and an increase in superoxide dismutase, catalase and ceruloplasmin activity and in lipid hydroperoxide content). Additionally, +7% versus the recommended Cu dietary level was followed by unfavorable results, indicating a deterioration in red blood cell parameters and stimulation of the immune system (an increase in interleukin-6, immunoglobulin A (IgA), IgM and IgY). To conclude, it was shown that it is possible to simultaneously increase antioxidant and immune defense of chickens by supplementing their diets with nano-Cu – up to 12 mg per bird during 6 weeks of feeding, that is to a level no more than 7% over the NRC recommendation for growing broiler chickens.
      PubDate: 2017-12-12T22:16:47.355597-05:
      DOI: 10.1111/asj.12956
  • Copper sulphate forms in piglet diets: Microbiota, intestinal morphology
           and enteric nervous system glial cells
    • Authors: Alessia Di Giancamillo; Raffaella Rossi, Piera Anna Martino, Lucia Aidos, Federica Maghin, Cinzia Domeneghini, Carlo Corino
      Abstract: The objective of this study was to evaluate dietary supplementation with different copper sulphate (CuSO4) forms on small intestine microanatomy and large intestine microbiota. Ninety weaned piglets were divided into three experimental groups: control diet (CTR), with no added CuSO4 and diets supplemented with 150 ppm of CuSO4 in protected (150P) and unprotected form (150UP). After 18 days of dietary treatment, six piglets per treatment were randomly selected and sacrificed. Duodenum villi length and crypt depths were higher (P 
      PubDate: 2017-12-12T05:49:13.530796-05:
      DOI: 10.1111/asj.12948
  • Effects of limit-feeding diets with different forage-to-concentrate ratios
           on nutrient intake, rumination, ruminal fermentation, digestibility, blood
           parameters and growth in Holstein heifers
    • Authors: Jun Zhang; Haitao Shi, Yajing Wang, Shengli Li, Hongtao Zhang, Zhijun Cao, Kailun Yang
      Abstract: The objective of this study was using a wide range of dietary concentrate levels to investigate the major effects of limit-feeding on heifers. Twenty-four Holstein heifers were blocked into six groups and fed with one of four diets containing different levels of concentrate (20%, 40%, 60% and 80% on a dry matter (DM) basis) but with same intakes of metabolizable energy for 28 days. Increasing levels of dietary concentrate caused decreased (P ≤ 0.02) intakes of dry matter (DMI) and neutral detergent fiber and total rumination time, but increased (P 
      PubDate: 2017-12-12T05:31:58.920945-05:
      DOI: 10.1111/asj.12959
  • Growth performance, carcass traits and meat quality of yellow-feathered
           broilers fed graded levels of alfalfa meal with or without wheat
    • Authors: Shouqun Jiang; Zhongyong Gou, Long Li, Xiajing Lin, Zongyong Jiang
      Abstract: The effects of 0, 40 and 80 g/kg alfalfa meal on growth performance, carcass traits and meat quality of Chinese yellow-feathered broilers fed diets containing or lacking wheat (0 or 200 g/kg) as part of the energy source, were examined using random design with a 2 × 3 factorial arrangement of treatments. Dressing percentage and semi-eviscerated proportion were lower, and meat color a* (redness) value was higher in birds fed diets containing wheat than diets lacking wheat (P 
      PubDate: 2017-12-07T03:20:38.862846-05:
      DOI: 10.1111/asj.12968
  • Thyroid hormones alter estrous cyclicity and antioxidative status in the
           ovaries of rats
    • Authors: Quanwei Wei; Jaafar Sulieman Fedail, Lingfa Kong, Kaizhi Zheng, Chunhua Meng, Mohamed Babo Fadlalla, Fangxiong Shi
      Abstract: To expand our understanding of the roles of thyroid hormones on female reproduction, we induced hypo- and hyper-T rat models to investigate the roles of thyroid hormones on estrous cyclicity, as well as the antioxidative status in the ovaries of rats. In the current study, our data show that hypothyroidism (hypo-T) and hyperthyroidism (hyper-T) led to significantly reduced body weights and ovarain weights and delayed vaginal opening day. For hyper-T, thyroxine (T4), tri-iodothyronine (T3), progesterone (P4) and follicle-stimulating hormone (FSH) were significantly increased, while estradiol (E2) and luteinizing hormone (LH) were significantly decreased. For hypo-T rats, serum levels of total T4 and T3, E2, P4, FSH and LH were significantly increased, while concentrations of E2 and LH were significantly decreased. For ovary morphology, the numbers of secondary and antral follicles were significantly decreased with more atretic antral follicles and less corpora lutea in both hyper- and hypo-T groups. Both hyper-T and hypo-T treatment significantly decreased the expressions of thyroid hormone receptor α1 in the ovary. Hypo-T significantly reduced nitric oxide (NO), total NO synthase (tNOS), inducible NOS and constitutive NOS activities, but hyper-T increased them. For antioxidative parameters, hypo-T and hyper-T treatment significantly increased malondialdehyde (MDA) contents. The activities of both glutathione peroxidase (GSH-Px) and catalase (CAT) significantly decreased in the hypo-T group but increased in the hyper-T group. Total superoxide dismutase (T-SOD) activity was significantly increased in the hyper-T group. In summary, thyroid hormones alter estrous cyclicity and antioxidative status in the ovary of the rat may act through the NOS signaling pathway.
      PubDate: 2017-12-07T02:56:30.135337-05:
      DOI: 10.1111/asj.12950
  • Characterization of myofibrillar adenosine triphosphatase activity and
           liberation of actin from myofibrils upon heating chicken breast meat
    • Authors: Masanori Matsuishi; Yoshitaka Eda, Emi Saito, Shohei Yamamoto, Kenji Kanamori, Yuto Goto, Yutaro Kobayashi, Akihiro Okitani
      Abstract: Denaturation of actin and myosin in myofibrils induced by heating at 50°C was investigated to reveal the mechanism of irreversible liberation of actin from myofibrils on heating at lower temperatures than conventional cooking. Denaturation of these proteins was determined by Mg2+-ATPase (adenosine triphosphatase) and Ca2+-ATPase activities. When minced meat was heated for 20 min, actin was liberated accompanying denaturation of 80% of actin and 50% of myosin. Heating of the myofibrillar fraction (MFF) isolated from meat homogenate induced much slower denaturation of actin than myosin. When MFF was heated with sarcoplasmic fractions, denaturation of actin was facilitated, suggesting that sarcoplasmic fractions contain factors to facilitate actin denaturation. Inosine-5′-monophosphate, a component of sarcoplasmic fractions, was shown to have no effect on actin and myosin denaturation. These results suggest that heating meat at 50°C dissociates binding (‘Bond A’) between actin and myosin participating in ATPase activities, resulting in denaturation of both proteins under influence of sarcoplasmic components. Although denaturation of actin and myosin disrupted Bond A, actin was not liberated simultaneously, suggesting the presence of another bond (‘Bond B’, more heat-stable than Bond A) between both proteins and necessity of disruption of Bond B for actin release from myofibrils.
      PubDate: 2017-12-07T01:21:24.057449-05:
      DOI: 10.1111/asj.12960
  • Effect of fermented total mixed rations on the growth performance, carcass
           and meat quality characteristics of Hanwoo steers
    • Authors: Tae-Il Kim; Vijayakumar Mayakrishnan, Dong-Hyun Lim, Jeong-Ha Yeon, Kwang-Soo Baek
      Abstract: This study was conducted to assess the effects of fermented total mixed ration (FTMR) on the growth performance, carcass and meat quality traits of Hanwoo steers. The present study evidenced that the FTMR had a strong effect on dry matter intake, body weight, daily gain, slaughter weight and carcass characteristics compared with control animals. The results showed that the dry matter intake (7.17 ± 0.13 kg), average body weight (615.20 ± 112.82 kg), and daily gain (0.56 ± 0.16 kg) were greater in animals receiving FTMR than in control animals (P 
      PubDate: 2017-12-04T06:25:00.809669-05:
      DOI: 10.1111/asj.12958
  • Effects of dietary protein level during rearing period and age at
           overfeeding on magret and foie gras quality in male mule ducks
    • Authors: Julien Arroyo; Caroline Molette, Franck Lavigne, Carole Margetyal, Olivier Amador, Jean-Pierre Dubois, Laurence Fortun-Lamothe
      Abstract: The aim of this trial was to study the effects of dietary protein content during the rearing period on the performance of mule ducks, according to age at overfeeding (O). Ducks (n = 612) were divided into four groups differing in the protein content in the diet offered during the starting period (S, 0–20 days; Sl vs. Sh: 150 vs. 175 g/kg crude protein (CP)) and growing-finishing period (GF; 21–67 or 81 days, depending on age at O; GFl vs. GFh: 133 vs. 152 g/kg CP). The relative weight of pectoral muscle was lower when ducks were fed a low protein diet during S (−5%, P 
      PubDate: 2017-12-04T06:20:23.766453-05:
      DOI: 10.1111/asj.12941
  • Different prostaglandin F2α secretion in response to oxytocin injection
           between pregnant and non-pregnant cows: effect of the day of oxytocin
           challenge test for determining the difference
    • Authors: Tomoaki Kubo; Kosuke Iga, Naoki Fukuju, Keiichiro Kizaki, Takeshi Osawa, Yoshiaki Izaike, Toru Takahashi
      Abstract: The present study was conducted to determine the difference in plasma prostaglandin F2α metabolite concentrations following oxytocin (OT) challenge between pregnant and non-pregnant cows. Experiment 1: cows were subjected to the OT challenge test on days 12, 14 or 16 (day of estrus = day 0) with or without prior insemination and plasma 13,14-dihydro-15-keto prostaglandin F2α (PGFM) concentrations were measured from −30 to 180 min after OT injection. On day 16, the increment of plasma PGFM concentrations in response to OT injection was significantly smaller in pregnant than that in cyclic cows. On days 12 and 14, there was little OT-induced PGFM secretion and no difference in PGFM increase between the pregnant and cyclic cows. Experiment 2: cows were inseminated on day 0 and subjected to the OT challenge test on day 16. Cows were classified into non-pregnant/early embryonic death (NP/EED), late embryonic death (LED) and pregnant (PREG) groups. The increment of PGFM concentrations in response to OT injection was less in both PREG and LED groups than that in the NP/EED group. In conclusion, plasma PGFM secretion induced by OT is suggested as the base of pregnancy diagnosis prior to returning estrus in cows.
      PubDate: 2017-11-27T04:16:40.93145-05:0
      DOI: 10.1111/asj.12952
  • Expression levels of taste-related genes in palate and tongue tip, and
           involvement of transient receptor potential subfamily M member 5 (TRPM5)
           in taste sense in chickens
    • Authors: Yuta Yoshida; Fuminori Kawabata, Yuko Kawabata, Shotaro Nishimura, Shoji Tabata
      Abstract: The elucidation of the mechanisms underlying the taste sense of chickens will contribute to improvements in poultry feeding, because the molecular mechanism of chickens’ taste sense defines the feeding behavior of chickens. Here we focused on the gene expressions in two different oral tissues of chickens – the palate, which contains many taste buds, and the tongue tip, which contains few taste buds. Using the quantitative real-time polymerase chain reaction method, we found that the molecular markers for taste buds of chickens, that is α-gustducin and vimentin, were expressed significantly highly in the palate compared to the tongue tip. Our analyses also revealed that transient receptor potential subfamily M member 5 (TRPM5), a cation channel involved in taste transduction in mammals, was also highly expressed in the palate compared to the tongue tip. Our findings demonstrated that the expression patterns of these genes were significantly correlated. We showed that the aversion to bitter solution was alleviated by a TRPM5 inhibitor in behavior of chickens. Taken together, our findings enabled us to develop a simple method for screening taste-related genes in chickens. The use of this method demonstrated that TRPM5 was involved in chickens’ taste transduction, and that a TRPM5 inhibitor can alleviate chickens’ bitter taste perception of feed ingredients.
      PubDate: 2017-11-27T03:58:57.109829-05:
      DOI: 10.1111/asj.12945
  • Effect of adding different levels of rumen protected choline to the diet
           on productive and reproductive performance of female goats and growth of
           their kids from birthing to weaning
    • Authors: Alsaied A. Habeeb; Ahmed E. Gad, Mostafa A. Atta, Mohammed M. Mustafa
      Abstract: Forty female goats in the third parity were randomly divided into four similar groups. The experiment was started 20 days before mating and lasted until the end of the suckling period for 60 days and weaning their kids. The first group were fed diet without supplementation and kept as control while in the second, third and fourth groups, each doe was fed diets with rumen protected choline (RPC) at the rate of 10, 20 and 40 g/day, respectively. Results showed that number of doe kidding twins and triplets and litter weight of kids born per group increased with increasing the level of RPC in the diet of goats and viability rate of born kids during the suckling period improved due to RPC supplementation in the diets of their mothers. Duration of estrous, days from weaning to estrous, days from kidding to estrous and kidding interval decreased significantly, while conception rate increased due to adding RPC. Milk choline concentrations and total choline secretion though milk were progressively increased significantly with increasing the level of RPC supplementation. Live body weight and daily body gain of their suckling male and female kids at weaning increased significantly with increasing RPC levels in the diets of their mothers.
      PubDate: 2017-11-20T07:00:19.154696-05:
      DOI: 10.1111/asj.12932
  • Effects of high ambient temperature on plasma metabolomic profiles in
    • Authors: Shozo Tomonaga; Hirofumi Okuyama, Tetsuya Tachibana, Ryosuke Makino
      Abstract: Exposure to high ambient temperature is detrimental to the poultry industry. To understand the influence from a metabolic perspective, we investigated the effects of exposure to high ambient temperature on plasma low-molecular-weight metabolite levels in chicks using gas chromatography/mass spectrometry-based non-targeted metabolomic analysis. Heat exposure for 4 days suppressed growth and food intake. Of the 92 metabolites identified, the levels of 29 decreased, whereas the levels of nine increased. We performed an enrichment analysis on the identified metabolites and found 35 candidates for metabolic processes affected by heat exposure. Among them, the sulfur amino acid metabolic pathway was clearly detected and the levels of the following related metabolites were decreased: cystathionine, cysteine, cystine, homocysteine and hypotaurine. Changes in the kynurenine pathway in tryptophan metabolism, which is linked to the immune system and oxidative stress, were also observed: kynurenine and quinolinic acid levels increased, whereas nicotinamide levels decreased. These results suggest the possible involvement of various metabolic processes in heat-exposed chicks. Some of these metabolites would be important to understand the mechanism of biological responses to high ambient temperature in chicks.
      PubDate: 2017-11-20T06:57:43.906228-05:
      DOI: 10.1111/asj.12951
  • Epistatic effects between pairs of the growth hormone secretagogue
           receptor 1a, growth hormone, growth hormone receptor, non-SMC condensin I
           complex, subunit G and stearoyl-CoA desaturase genes on carcass,
           price-related and fatty acid composition traits in Japanese Black cattle
    • Authors: Masanori Komatsu; Kagetomo Nishino, Yuki Fujimori, Yasutoshi Haga, Nagako Iwama, Aisaku Arakawa, Yoshito Aihara, Hisato Takeda, Hideaki Takahashi
      Abstract: Growth hormone secretagogue receptor 1a (GHSR1a), growth hormone (GH), growth hormone receptor (GHR), non-SMC condensin I complex, subunit G (NCAPG) and stearoyl-CoA desaturase (SCD), are known to play important roles in growth and lipid metabolisms. Single and epistatic effects of the five genes on carcass, price-related and fatty acid (FA) composition traits were analyzed in a commercial Japanese Black cattle population of Ibaraki Prefecture. A total of 650 steers and 116 heifers for carcass and price-related traits, and 158 steers for FA composition traits were used in this study. Epistatic effects between pairs of the five genes were found in several traits. Alleles showing strain-specific differences in the five genes had significant single and epistatic effects in some traits. The data suggest that a TG-repeat polymorphism of the GHSR1a.5′UTR-(TG)n locus plays a central role in gene–gene epistatic interaction of FA composition traits in the adipose tissue of Japanese Black cattle.
      PubDate: 2017-11-20T06:57:38.179703-05:
      DOI: 10.1111/asj.12947
  • Influences of quorum-quenching probiotic bacteria on the gut microbial
           community and immune function in weaning pigs
    • Authors: Jonggun Kim; Jaepil Kim, Younghoon Kim, Sangnam Oh, Minho Song, Jee Hwan Choe, Kwang-Youn Whang, Kwang Hyun Kim, Sejong Oh
      Abstract: The aim of this study is to investigate the dynamic gut microbial diversity in weaning swine after administering feed supplemented with probiotic bacteria that specifically inhibit the activity of quorum molecules. Initially, the universal quorum molecule autoinducer-2 (AI-2) bioassay results indicated that AI-2 activity was profoundly inhibited in enterohemorrhagic Escherichia coli (EHEC) O157:H7 in the presence of Lactobacillus acidophilus strain 30SC cell extract, although the growth of EHEC was not affected. Based on plate counting results, bacterial community analysis revealed a specific reduction in coliforms compared to the control, whereas the population of lactobacilli increased in weaning swine in in vivo trials. Supplementation with L. acidophilus strain 30SC did not affect the counts of other communities, such as total aerobes and yeast/mold. In addition, PCR-denaturing gradient gel electrophoresis analysis showed a significant difference in the 16S rRNA gene products after administering L. acidophilus strain 30SC. Selected bands were sequenced, and most of them were identified as uncultured bacterium clones or a Lactobacillus- and Bifidobacterium-specific community. Therefore, our results indicate that quorum-quenching probiotic bacteria can significantly modulate the gut microbiota of swine and these beneficial effects can contribute to the improvement of performance and health in the gastrointestinal tract of weaning pigs.
      PubDate: 2017-11-20T06:50:40.088837-05:
      DOI: 10.1111/asj.12954
  • Development of furnished cages re-using conventional cages for laying
           hens: Behaviour, physical condition and productivity
    • Authors: Tsuyoshi Shimmura; Naoko Maekawa, Satoshi Hirahara, Toshio Tanaka, Michael C. Appleby
      Abstract: Furnished cages for laying hens have advantages in allowing normal behaviors and maintaining productivity. As the cost of introduction is a barrier for farms, we developed furnished cages that re-use conventional cages. To determine the minimum and functional cage design, we compared six designs, combinations of two floor designs (artificial turf or wire cage floor) and three screening designs in the integrated area (no screening, one entrance side or four sides). In total, 144 hens were used, and we measured behavior, physical condition and productivity. Comparing the floors, the percentages of hens performing dust-bathing and laying eggs in the integrated area were higher in cages with turf than wire floor (P 
      PubDate: 2017-11-20T06:50:21.375539-05:
      DOI: 10.1111/asj.12955
  • Effects of supplementary amino acids on available energy of soybean meal
           determined by difference and regression methods fed to growing pigs
    • Authors: Jinbiao Zhao; Chuanxin Shi, Zhongchao Li, Ji Wang, Ling Liu, Defa Li, Shuai Zhang
      Abstract: This study was conducted to evaluate effects of inclusion level and supplementary amino acids (AAs) on digestible energy (DE) and metabolizable energy (ME) values of soybean meal (SBM) fed to growing pigs, determined by difference and regression methods. Sixty pigs were fed 10 diets according to a 5 × 2 factorial arrangement. Two control diets contained 97.34% corn without supplementary AAs or 95.61% corn with supplementary AAs. Eight diets were formulated by replacing corn and AAs in control diets with 8%, 15%, 25% and 31% SBM. There was no difference in DE and ME values of SBM determined by difference method as inclusion level of SBM increased or crystalline AAs were added. No difference was observed in DE and ME values of SBM determined by the two methods in diets without supplementary AAs, but the values determined by the difference method were greater (P 
      PubDate: 2017-11-12T22:25:23.098521-05:
      DOI: 10.1111/asj.12940
  • Performance and hemtochemical parameters of buck-kids fed concentrate
           partially replaced with tropical Piliostigma thonningii foliage
    • Authors: Olurotimi A. Olafadehan; Ahmed A. Njidda, Sunday A. Okunade, Sarah O. Salihu, David O. Balogun, Abdelfattah Z. M. Salem
      Abstract: Fifteen 5-month-old Red Sokoto buck-kids, (6.6 ± 0.71 kg body weight (BW)) randomly distributed into three groups of five animals per group, were used to study the effects of supplementary concentrate partially replaced with Piliostigma thonningii (PT) foliage on the growth performance, economic benefit and blood profile in a completely randomized design using analysis of variance. The goats in group 1 received 100% supplementary concentrates (PT0), groups 2 and 3 received 25% (PT25) and 50% (PT50), respectively, of concentrate replaced with an equal amount (dry matter basis) of Piliostigma foliage. The goats were fed a basal diet of threshed sorghum top (TST). Intake of concentrate, hemoglobin, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, total feeding cost and cost/kg BW were greater (P 
      PubDate: 2017-11-12T21:35:20.403409-05:
      DOI: 10.1111/asj.12814
  • Effect of follistatin on pre-implantational development of pig
           parthenogenetic embryos
    • Authors: Yunsheng Li; Xing Liu, Zhen Chen, Dandan Song, Jie Yang, Xiaoyuan Zuo, Zubing Cao, Ya Liu, Yunhai Zhang
      Abstract: The present study was designed to explore effects of follistatin (FST) on pre-implantational development of parthenogenetically activated embryos (PAEs) in pigs. First, we investigated the FST messenger RNA expression level and dynamic FST protein expression patterns in porcine oocytes and PAEs. Then, PAEs were placed in embryo culture medium supplemented with 10 ng/mL of FST-288, FST-300, and FST-315. Next, PAEs were cultured with 0, 1, 10 and 100 ng/mL of FST-315 protein throughout the in vitro culture (IVC) duration. Further, 10 ng/mL of FST-300 was added from the start of IVC in which PAEs were treated for 30, 48 and 60 h. The results showed that 1 ng/mL FST-315 could significantly increase the total cell numbers of blastocyst and trophectoderm cell number in PAEs. Exogenous FST-300 supplementation could significantly promote the early cleavage divisions and improve the blastocyst formation rate of porcine embryos. FST-300 appeared to affect early embryonic development before activation of the embryonic genome. In all, the study confirmed for the first time that FST plays a role in promoting early embryonic development in pigs, which differed with different FST subtypes. FST-300 could facilitate the initial cleavage time and improve the blastocyst formation rate, and FST-315 could improve the blastocyst quality.
      PubDate: 2017-11-09T01:30:47.489465-05:
      DOI: 10.1111/asj.12936
  • Relationship of the conception rate and the side (left or right) of
           preovulatory follicle location at artificial insemination in dairy heifers
    • Authors: Ryotaro Miura; Taiki Izumi
      Abstract: In this study, we examined the locational effect (left or right ovary) of the preovulatory follicle (PF) on fertility in dairy heifers. In total, 1,111 artificial inseminations (AI) were analyzed. At AI, PF locations were examined using rectal palpation, and heifers were divided into two groups on their PF locations: (i) the PF located in the left ovary (L-PF); and (ii) the PF located in the right ovary (R-PF). Pregnancy was diagnosed by rectal palpation 60 days after AI. The conception rate was 50.7% in all heifers. Conception rate was significantly higher in the L-PF (60.1%) than in the R-PF (46.2%). The conception rate was significantly lower by sexed semen (48.6%) than conventional semen (59.1%). Conception rates divided by the semen type (sexed: n = 896, conventional: n = 215) were significantly higher in the L-PF than in the R-PF for both semen types (sexed; L-PF vs. R-PF: 57.3% vs. 44.4%, conventional; L-PF vs. R-PF: 72.3% vs. 53.3%). In addition, season, age, AI number, and the number of re-inseminations at the same estrus did not affect conception rates. In summary, PF development in the left ovary was associated with increased conception rates in dairy heifers.
      PubDate: 2017-11-08T04:55:25.580776-05:
      DOI: 10.1111/asj.12949
  • Trans10, cis12 conjugated linoleic acid increases triacylglycerol
           accumulation in goat mammary epithelial cells in vitro
    • Authors: Tianying Zhang; Yue Ma, Hui Wang, Juan J Loor, Huifen Xu, Huaiping Shi, Jun Luo
      Abstract: Trans10, cis12 conjugated linoleic acid (t10c12-CLA) is well-established in decreasing milk fat content and causing milk fat depression (MFD) in dairy cattle and goats. However, the detailed mechanisms of its effect are not completely understood. Therefore, we used goat mammary epithelial cells (GMECs) to further study the molecular mechanisms whereby t10c12-CLA regulates milk fat synthesis. The optimal concentration of t10c12-CLA (100 μmol/L) for cell culture was determined through a cell vitality and morphology assay, and evaluation of abundance of apoptosis-related proteins. Oil red O stain indicated that t10c12-CLA increased concentration of cytoplasmic lipid droplets. Furthermore, t10c12-CLA increased the intracellular triacylglycerol (TG) content (P 
      PubDate: 2017-11-08T04:30:50.546201-05:
      DOI: 10.1111/asj.12935
  • Impact of α-amylase supplementation on energy balance and performance of
           high-yielding dairy cows on moderate starch feeding
    • Authors: Martin Bachmann; Mandy Bochnia, Norbert Mielenz, Joachim Spilke, Wolfgang B. Souffrant, Elisabeth Azem, Wolfgang Schliffka, Annette Zeyner
      Abstract: In dairy cows, exogenous α-amylase is suggested to improve starch utilization and positively affect performance and health traits linked to energy balance and fertility. In a 1-year feeding experiment, 421 cows were orally supplemented with α-amylase (treatment: 12.5 g/kg dry matter (DM) addition rate to a concentrated feed) or non-supplemented (control) on the basis of an ad libitum total mixed ration (TMR). Every cow was allocated to a high- (≥32 kg milk/day) or late-lactation group (
      PubDate: 2017-10-30T05:00:20.953773-05:
      DOI: 10.1111/asj.12939
  • Effects of composite antimicrobial peptide on growth performance and
           health in weaned piglets
    • Authors: Jiankai Shi; Pan Zhang, Meng meng Xu, Zhengfeng Fang, Yan Lin, Lianqiang Che, Bin Feng, Jian Li, Gang Li, De Wu, Shengyu Xu
      Abstract: This study was conducted to evaluate the effects of composite antimicrobial peptide (CAP) on growth performance and health status in weaned piglets. Over 28 days, 36 weaned piglets (body weight, 10.58 ± 0.99 kg) underwent three treatments: negative control (NC, basal diet), positive control (PC, basal diet + 20 mg/kg colistin sulphate + 50 mg/kg kitasamycin), and CAP treatment (CAP, basal diet with 400 mg/kg CAP). Average daily gain of piglets fed the CAP diet was greater (P 
      PubDate: 2017-10-30T00:50:22.617523-05:
      DOI: 10.1111/asj.12933
  • Influence of stewing time on the texture, ultrastructure and in vitro
           digestibility of meat from the yellow-feathered chicken breed
    • Authors: Jun Qi; Xiao Li, Wenwen Zhang, Huhu Wang, Guanghong Zhou, Xinglian Xu
      Abstract: This study assessed the influence of stewing (1, 2 or 3 h) on the texture, ultrastructure and in vitro digestibility of meat from the yellow-feathered chicken, which is a popular broiler chicken in Asia. Results indicated that longer stewing considerably increased cooking loss of the chicken carcass and tenderness of thigh meat. After 3 h of stewing, protein digestibility decreased from 90.5% to 80.3% and fiber diameter decreased by 8.63 μm. The shear force value of breast meat decreased from 32.34 N at 1 h to 10.29 N at 2 h, and then increased to 39.98 N at 3 h. The texture profile of breast meat remarkably decreased during stewing. Moreover, increased stewing time induced longitudinal and transversal shrinkage of muscle fibers and the degradation of the myosin heavy chain. These findings suggested that prolonged stewing (3 h) resulted in decreased meat qualities, based on the changes in cooking loss, digestibility and texture profile, but that stewing for 2 h increased thigh and breast tenderness. Producers could utilize this information to stew yellow-feathered chicken meat with desirable qualities.
      PubDate: 2017-10-30T00:30:51.756955-05:
      DOI: 10.1111/asj.12929
  • Involvement of μ/m-calpain in the proteolysis and meat quality changes
           during postmortem storage of chicken breast muscle
    • Authors: Liang Zhao; Tong Xing, Jichao Huang, Yan Qiao, Yulian Chen, Ming Huang
      Abstract: The objective of this study was to investigate the role of calpain isotypes, especially poultry-specific μ/m-calpain in the proteolysis and meat quality changes of chicken breast muscle during postmortem storage. Calpain activity was detected by casein zymography, while the degradation of titin, desmin and Troponin-T was analyzed by sodium dodecyl sulfate – polyacrylamide gel electrophoresis and western blot. Meat quality indicators such as water holding capacity and tenderness were also studied. The correlation analysis between calpain activity, proteolysis and the changes in meat quality indicators indicated that there were strong correlations for μ-calpain during the first 12 h of storage, while such strong correlations for μ/m-calpain were only found in samples stored from 12 h to 7 days. Our study suggested that μ-calpain played a major role in meat quality changes while μ/m-calpain could also be involved but played a limited role in the proteolysis and meat quality changes during 12 h to 7 days postmortem storage of chicken breast muscle.
      PubDate: 2017-10-26T19:45:34.558232-05:
      DOI: 10.1111/asj.12921
  • A method for extracting carboxy-myoglobin from beef
    • Authors: Masahiro Waga; Shiro Takeda, Ryoichi Sakata
      Abstract: The present study was conducted to devise a method for the effective extraction of carboxy-myoglobin (COMb) from beef without carbon monoxide dissociation. The ratio of COMb to myoglobin was computed at absorptions of wavelengths 541 and 551 nm, which characterize COMb and the isosbestic point between COMb and deoxy-myoglobin, respectively. The COMb extraction rate was found to vary with temperature, pH and oxygen conditions. The decrease observed in this rate was inversely proportional to the rise in extraction temperature. The COMb extraction rate was also affected by pH, and the stability of COMb in the extract solution was the highest at pH 8.0–9.0. Moreover, the presence of oxygen was found to disturb COMb extraction. According to these results, nearly all COMb could be extracted from carbon-monoxide-treated beef under stirring conditions in pH 8.5 deoxidized buffer, at 1°C, and under N2 flow with the improved extraction method in this study (98.1 ± 2.7%). The decrement of COMb in the extract was accelerated by light, and the COMb was stable for 20 min in the dark, at 1°C. The extraction conditions for COMb described above should allow the accurate evaluation of COMb in meat tissue.
      PubDate: 2017-10-20T04:30:25.003404-05:
      DOI: 10.1111/asj.12942
  • Interactions between fawns and does of farmed fallow deer Dama dama in the
           postnatal period
    • Authors: Paweł Janiszewski; Justyna Cilulko-Dołęga, Daria Murawska, Marek Bogdaszewski
      Abstract: The aim of the present study was to describe the suckling behavior of the offspring of farmed European fallow deer Dama dama in the postnatal period. Video surveillance footage from the first 18 days postpartum was analyzed in detail to reveal that the duration of interactions between the mother and the offspring changed with the fawns’ age. The duration of mother-offspring interactions was longest between postpartum days 12 and 18. The frequency of interactions at different times of the day varied in the analyzed periods. The results of the study could be used to increase the welfare of farmed deer.
      PubDate: 2017-10-18T22:10:35.126087-05:
      DOI: 10.1111/asj.12926
  • Partial characterization of phylogeny, ecology and function of the
           fibrolytic bacterium Ruminococcus flavefaciens OS14, newly isolated from
           the rumen of swamp buffalo
    • Authors: Phoompong Boonsaen; Madoka Kinjo, Suriya Sawanon, Yutaka Suzuki, Satoshi Koike, Yasuo Kobayashi
      Abstract: The fibrolytic rumen bacterium Ruminococcus flavefaciensOS14 was isolated from swamp buffalo and its phylogenetic, ecological and digestive properties were partially characterized. Isolates from rumen contents of four swamp buffalo were screened for fibrolytic bacteria; one of the 40 isolates showed a distinctive feature of solubilizing cellulose powder in liquid culture and was identified as R. flavefaciens based on its 16S ribosomal DNA sequence. This isolate, OS14, was employed for detection and digestion studies, for which a quantitative PCR assay was developed and defined cultures were tested with representative forages in Thailand. OS14 was phylogenetically distant from other isolated and uncultured R. flavefaciens and showed limited distribution among Thai ruminants but was absent in Japanese cattle. OS14 digested rice straw and other tropical forage to a greater extent than the type strain C94 of R. flavefaciens. OS14 produced more lactate than C94, and digested para grass to produce propionate more extensively in co-culture with lactate-utilizing Selenomonas ruminantium S137 than a co-culture of C94 with S137. These results indicate that phylogenetically distinct OS14 could digest Thai local forage more efficiently than the type strain, possibly forming a symbiotic cross-feeding relationship with lactate-utilizing bacteria. This strain might be useful for future animal and other industrial applications.
      PubDate: 2017-10-17T04:00:42.684025-05:
      DOI: 10.1111/asj.12927
  • Selenium supplementation during in vitro maturation enhances meiosis and
           developmental capacity of yak oocytes
    • Authors: Xianrong Xiong; Daoliang Lan, Jian Li, Yaqiu Lin, Mingyang Li
      Abstract: The objectives of the present study were to investigate the effects of selenium (Se) supplementation during in vitro maturation (IVM) on the developmental capacity of yak (Bos grunniens) oocytes. Nuclear maturation, DNA integrity, glutathione peroxidase (GSH-Px) activity, subsequent embryonic development, and gene expression after in vitro fertilization (IVF) were evaluated. The Se concentrations in yak plasma and follicular fluid were 0.142 and 0.069 μg/mL, respectively. The DNA damage in cumulus cells decreased significantly with 2 and 4 μg/mL supplementation of sodium selenite to IVM medium (P  0.05). The IVF blastocyst formation rates of 0, 1 and 4 μg/mL sodium selenite groups were 47.7%, 51.2% and 58.9%, respectively. The 2 μg/mL sodium selenite group had the highest blastocyst formation rate (60.5%). Gene expression analysis revealed that the quantity of transcripts associated with selenoprotein and protein synthesis were high in the 2 and 4 μg/mL groups. In conclusion, both GSH-Px activity of oocytes and DNA integrity of cumulus cells significantly increased with supplemental Se during oocyte IVM. Considering that embryonic development is responsive to Se supplementation, we inferred that appropriate Se concentrations during IVM were beneficial for yak oocyte maturation and subsequent development.
      PubDate: 2017-10-16T03:55:52.485355-05:
      DOI: 10.1111/asj.12894
  • Feeding forage mixtures of alfalfa hay and maize stover optimizes growth
           performance and carcass characteristics of lambs
    • Authors: Lin Sun; Qiang Yin, Ge Gentu, Yanlin Xue, Meiling Hou, Liying Liu, Yushan Jia
      Abstract: A feeding experiment was carried out to determine the effects of different proportions of alfalfa hay and maize stover on the growth performance and carcass characteristics of fattening lambs. Forty-two healthy 3.5-month-old male Small-tail Han lambs of 23.89 ± 0.38 kg body mass were assigned to seven groups, which were fed mixed diets composed of maize stover and alfalfa hay containing 0%, 30%, 40%, 50%, 60%, 70% or 100% maize stover on a dry matter (DM) basis. Each lamb received 1.5 kg DM of forage mixture three times daily and supplementary concentrate (0.5 kg corn per lamb per day) for 60 days. Compared with the control forage diet of maize stover only, forage intake rate and forage conversion rate on the 40% maize stover/60% alfalfa hay mixture were 30% and 5% higher, respectively. Body weight, dressing percentage and net income were 6.7 kg, 8% and 94.37 Yuan higher per lamb, respectively. Thus, feeding lambs with a forage mixture of maize stover and alfalfa hay in a ratio of 40:60 optimized the production performance, slaughter performance, and the income derived from lambs.
      PubDate: 2017-10-11T01:05:23.541801-05:
      DOI: 10.1111/asj.12928
  • Optimization and physicochemical properties of nutritional protein isolate
           from pork liver with ultrasound-assisted alkaline extraction
    • Authors: Ye Zou; Huan Bian, Pengpeng Li, Zhilan Sun, Chong Sun, Muhan Zhang, Zhiming Geng, Weimin Xu, Daoying Wang
      Abstract: The aim of this study was to investigate the optimal conditions of ultrasound-assisted alkaline extraction (UAAE) on pork liver protein isolate (UPLPI) and its physico-chemical properties. Response surface methodology was used to determine the optimal conditions for UAAE, which were at ultrasonic power 265 W, ultrasonic time 42 min, NaOH concentration 0.80%, temperature 50°C, and solvent/raw material ratio 70. The extraction yield and efficiency of UPLPI were significantly improved over the conventional alkaline extraction (PLPI). The results of amino acid composition showed that UAAE could increase serine (36.5 g/kg), arginine (38.1 g/kg), alanine (37.5 g/kg), proline (48.7 g/kg), phenylalanine (55.6 g/kg) and lysine (47.2 g/kg) elution amount. The changes in fourier transform infrared spectra indicated unfolding and destruction of the protein structure in UPLPI. The differential scanning calorimetry analysis presented UPLPI with a slightly lower onset and peak denaturation temperature over PLPI. Surface hydrophobicity increased and the microstructures presented larger and more pores of UPLPI, therefore, it had better in vitro digestibility than PLPI. Therefore, UPLPI might have a potential application prospect in the food field due to its changes on molecular structure as well as on the microstructure of protein by UAAE.
      PubDate: 2017-10-10T03:21:11.885976-05:
      DOI: 10.1111/asj.12930
  • Vitrification of bovine matured oocytes and blastocysts in a paper
    • Authors: Ashit Kumar Paul; Yuanyuan Liang, Kanokwan Srirattana, Takashi Nagai, Rangsun Parnpai
      Abstract: In the present study, we aimed to determine the applicability of a paper container for the vitrification of in vitro matured (IVM) bovine oocytes. In experiment 1, IVM oocytes were exposed to vitrification solution (20% dimethylsulfoxide (DMSO), 20% ethylene glycol (EG), and 5 mol/L sucrose), using a two-step method, for 30 s; loaded onto either a paper container or Cryotop; and stored in liquid nitrogen. No significant difference (P 
      PubDate: 2017-10-10T03:20:57.600841-05:
      DOI: 10.1111/asj.12892
  • Effect of replacing alfalfa hay with a mixture of cassava foliage silage
    • Authors: Hoang Lam Dang; Renlong Lv, Taketo Obitsu, Toshihisa Sugino
      Abstract: This study aimed to investigate the effects of replacing alfalfa hay (AH) with a mixture of cassava foliage silage and sweet potato vine silage (CSP) (1:1 on a dry matter (DM) basis) on ruminal and intestinal nutrient digestion in sheep. Four wethers were fed a control diet containing 35% of AH and two treatment diets containing 15% and 30% of the CSP as substitute for AH at 1.5 times the metabolizable energy required for maintenance. Replacing AH with the CSP silage did not affect DM intake, whereas it linearly increased (P 
      PubDate: 2017-10-09T20:41:02.158437-05:
      DOI: 10.1111/asj.12925
  • The use of accelerometer to measure sleeping posture of beef cows
    • Authors: Michiru Fukasawa; Tokushi Komatsu, Yumi Higashiyama, Akinori Oshibe
      Abstract: Sleep is one of the essential behaviors for mammals. The aims of this study were to validate the use of accelerometer for measuring sleeping posture of cattle. Duration of sleeping posture of seven Japanese Black cows from 19.00 to 07.00 hours was measured by both accelerometer and video, and a total of 67 accelerometer and video measurement sets were collected. We calculated Cohen's κ coefficient between accelerometer and video measurements and 91.5% of the κ-values were>0.80. Intra- and inter-observer coefficient of variance showed that specific acceleration waveform patterns of sleeping posture could be easily and accurately detected by independent observers. There were no significant differences in the frequency of sleeping posture occurrences between accelerometer and video measurements. We compared averaged sleeping posture bout, and the total sleeping posture time between accelerometer and video measurements using regression. In each trait, the slope was close to 1 and the intercept was not different from 0, which showed a strong agreement between accelerometer and video measurements. This shows that an accelerometer could accurately detect sleeping postures of cattle. We conclude that adequate measurements of sleeping postures can be made using an accelerometer.
      PubDate: 2017-10-09T20:40:59.308083-05:
      DOI: 10.1111/asj.12931
  • Hair cortisol levels of lactating dairy cows in cold- and warm-temperate
           regions in Japan
    • Authors: Katsuji Uetake; Shigeru Morita, Nobutada Sakagami, Kazuaki Yamamoto, Shinji Hashimura, Toshio Tanaka
      Abstract: We compared the hair cortisol levels of lactating dairy cows in a cold- and a warm-temperate region out of four climatic zones in Japan. We simultaneously investigated the effects of calving number, lactation period and month of hair sampling. Hair of nine Holstein lactating cows chosen from each region (i.e. 18 cows per sampling) was sampled in March, June, September and December. Number of calvings (1, 2, ≥3) and lactation duration (early: 201 days) were balanced between regions. Cortisol was extracted from hair by methanol, and its level was determined with a cortisol immunoassay kit. A multi-way analysis of variance revealed that the effects of month of hair sampling (P 
      PubDate: 2017-10-09T20:40:37.889071-05:
      DOI: 10.1111/asj.12934
  • Aging adult porcine fibroblasts can support nuclear transfer and
           transcription factor-mediated reprogramming
    • Authors: Xia Li; Pengfei Zhang, Shaoshuai Jiang, Biao Ding, Xiaoyuan Zuo, Yunsheng Li, Zubing Cao, Yunhai Zhang
      Abstract: Somatic cell nuclear transfer (SCNT) and induced pluripotent stem cells (iPSCs) technology are two classical reprogramming methods. Donor cell types can affect the reprogramming results in the above two methods. We here used porcine embryonic fibroblasts (PEFs) and adult porcine ear skin fibroblasts (APEFs) and adipose-derived stem cells (ADSCs) as donor cells for SCNT and source cells for iPSCs to study their in vitro developmental capability and colony-formation efficiency, respectively. For SCNT, fusion and cleavage rate has no significant difference among PEFs, ADSCs and APEFs. The rate and total cell number of blastocysts in the APEF group were significant lower than that in PEFs and ADSCs. For transcription factor-mediated reprogramming, the reprogramming efficiency of ADSCs were significantly higher than PEFs and APEFs and there is no significant difference between PEFs and APEFs. Furthermore, PEFs, APEFs and ADSCs can be used to generate iPSCs. Fianlly, somatic cloned pigs could still be successfully generated from APEFs, suggesting terminally differentiated aging adult somatic cells could be reprogrammed into a totipotent state. Considering the easy availability of animal tissue and the costs of establishing cell lines, aging porcine ear fibroblasts can support nuclear transfer-mediated and transcription factor-based reprogramming.
      PubDate: 2017-10-03T01:35:14.728104-05:
      DOI: 10.1111/asj.12871
  • The induction of cashmere shedding via cyclophosphamide injection
    • Authors: Qian Chen; Yu-yan Cong, Xue-jian Li, Rui-yang Zhang
      Abstract: The aim of this study was to investigate the effects of cyclophosphamide (CPA) on cashmere shedding in cashmere goats. Thirty-two castrated Liaoning cashmere goats were randomly allotted to four groups, with eight replicates in each group. The four groups were injected intravenously with CPA doses of 0, 15, 20 and 25 mg/kg body weight, respectively. Feed intake, body weight, body temperature, and sphygmus were recorded and the erythrocyte count, leukocyte count, hemoglobin content, and cashmere yield and length were determined. CPA has no significant effect on feed intake, body weight, body temperature, or sphygmus of cashmere goats. It was found that CPA significantly decreased the erythrocyte count and hemoglobin content in cashmere goats on the days immediately following injection, but the effects on erythrocytes diminished within 6 days, with hemoglobin content returning to normal within 10 days. Cashmere fiber began to shed on about day 10 after injection with CPA. CPA had no significant effect on cashmere length but significantly increased cashmere yield. The results indicate that CPA can induce cashmere shedding and achieve the purpose of concentrated defleecing. A dose of 20 mg/kg body weight is preferable for hair removal and regrowth in cashmere goats.
      PubDate: 2017-10-01T21:05:31.066858-05:
      DOI: 10.1111/asj.12917
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