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EUREKA : Health Sciences
Number of Followers: 2  

  This is an Open Access Journal Open Access journal
ISSN (Print) 2504-5660 - ISSN (Online) 2504-5679
Published by Scientific Route OÜ Homepage  [8 journals]
  • GASTROESOPHAGEAL REFLUX DISEASE AND BRONCHIAL ASTHMA: MECHANISMS OF
           COMORBID FORMATION AND COURSE.

    • Authors: Aleksey Oparin, Givi Akhvlediani, Anatoliy Oparin, Sergei Pavlov
      Pages: 3 - 7
      Abstract: The aim of the study: To estimate the level of Galectin-3 with the parallel tracing of the content of pro- and anti-inflammatory cytokines Interleukin-6 (IL-6) and Interleukin-4 (IL-4) for patients with GERD and BA both in case of separate nosologies, and in case of their combined course during the period of exacerbation of the diseases.Methods. The study was conducted in three groups of patients, homogeneous by gender and age. The first group included 18 patients with GERD. The second group included 19 patients with intermittent or persistent-mild bronchial asthma. The third group included 22 patients suffering from GERD with concomitant BA intermittent or persistent-mild severity. Determination of the level of galectin-3 and interleukins (IL-4 and IL-6) in the blood serum was carried out by enzyme-linked immunosorbent assay.Results. Analyzing the results of the study, we found that the level of galectin - 3 was increased on average in both groups of patients with isolated GERD (and in patients with BA). In patients of the third group with comorbid pathology, the level of galectin-3 was statistically significantly higher than not only the norm, but also the average of patients with isolated BA and GERD. At the same time, we found the rise in the level of pro-inflammatory (IL-6) and anti-inflammatory (IL-4) cytokines. Moreover, in patients with GERD, the level of IL-6 was increased with a higher degree of reliability, and the level of IL-4 was increased with a lower degree of reliability. In patients with BA, on the contrary, the level of IL-4 was determined more often and higher, and the level of IL-6 was lower.Conclusions. Analyzing result of the study, a clear correlation and features of changes in the level of galectin-3, IL-4, IL-6 in patients with isolated GERD, BA, as well as with the comorbidity of these diseases, were revealed.In patients with BA, the level of galectin-3 increases with the same degree of certainty as in the group of patients with GERD. In the cytokine system, on the contrary, the level of anti-inflammatory (IL-4) cytokines increases with a greater degree of certainty than the level of pro-inflammatory (IL-6) cytokines.In patients with GERD with concomitant BA, the level of galectin-3 increases with a greater degree of certainty. It is observed also a higher rising of pro-inflammatory (IL-6) cytokines and a slightly pronounced increasing of the level of anti-inflammatory (IL-4) cytokines in comparison with the group of patients with isolated GERD.
      PubDate: 2020-03-31
      DOI: 10.21303/2504-5679.2020.001199
       
  • IMMUNE STATUS OF PATIENTS WITH LUNG TUBERCULOSIS AND ALCOHOL CONSUMPTION

    • Authors: Maryna Kochuieva, Ivan Hrek, Olena Klimova, Anton Rogozhyn, Vasyl Kushnir
      Pages: 8 - 16
      Abstract: Tuberculosis is a great medical and social problem. An important risk factor for tuberculosis progress is the systematic use of alcohol. The relationship between alcohol intake and TB has been clear after numerous studies and meta-analyzes. However, there are still open questions about whether alcohol is the direct cause of TB development, which doses of alcohol have the greatest trigger value in progress of TB process, what kind of immunological reactions are significantly associated with alcohol intake and what immune background can determining role of alcohol in progression of TB infection. The features of the immune response in TB patients with different value of alcohol use remain poorly understood.The aim of the research - to investigate the immune status in patients with firstly diagnosed pulmonary tuberculosis with various levels of alcohol consumption.Materials and methods. We selected for participating in the study 102 males with tuberculosis and alcohol consumption. Examination of the patients included: clinical methods, chest X-ray, sputum tests, Alcohol Use Disorders Identification Test and immunological blood analysis.Results. Patients with tuberculosis and alcohol consumption, have increase inflammatory response: higher level of stab neutrophils, CRP, ESR, as well as phagocyte activity, circulating immune complexes levels and medium molecular weight peptides levels. On the other hand we found decreasing of phagocytic number, index of phagocytosis completeness and differentiation clusters CD2+, CD3+, CD4+, CD8+ of T-lymphocytes` sub-populations. The highest changes were observed in the group of alcohol abusers.Conclusions. Patients with tuberculosis and alcohol intake, even at low levels of its consumption, have a dysregulation of their immune response. On the one hand, there is an increase of non-specific inflammatory reactions, and on the other - a situation, when antigen-presenting cells cannot adequately to process and present the antigen to phagocytes. This prevents the immune system from responding quickly, correctly evaluating and suppressing foreign agents.
      PubDate: 2020-03-31
      DOI: 10.21303/2504-5679.2020.001177
       
  • COMPARING OF FAECAL CALPROTECTIN LEVELS IN PATIENTS WITH OSTEOARTHRITIS
           TAKING NSAID TREATMENT AND PATIENTS WITHOUT NSAIDS THERAPY

    • Authors: Olena Gubska, Andrii Kuzminets, Artem Panin
      Pages: 17 - 24
      Abstract: Faecal calprotectin (FC) level can be increased in several conditions, which are characterised by neutrophilic inflammation. Some medications, particularly NSAIDs, can elevate its level as well. NSAIDs are taken by patients in many chronic conditions, including osteoarthritis (OA).On the other hand, there is growing evidence that osteoarthritis is not only a degenerative disease, but it has a significant inflammatory component. The role of systemic inflammation is well-known in inflammatory joint diseases, but there is some evidence that it can play an essential role in the OA as well. It can suggest that in the OA, the inflammatory changes could be found in the different organs and systems.The aim of this study was to investigate the FC level in patients with osteoarthritis depending on the NSAIDs intake and to compare it to the FC levels in healthy adults.Materials and methods. In this small observational study, we evaluated the FC levels in patients suffering from OA (36 persons), divided them into two groups depending on their NSAIDs intake, and compared it to FC levels in healthy participants (12 persons). We compared the FC levels depending on the selectivity of the NSAIDs taken by our participants, as well.Results. The median calprotectin level in our patients was 72.57 (IQR 20.55-221.57) mg/kg, 95 %CI 26,18-109.01. OA patients had higher levels than the healthy group (p<0.001). OA patients who took NSAIDs had the highest FC levels – 221.57 (IQR 91.56-448.61) – higher, than those who did not take it – 72.57 (IQR 35.26-164.79) (p=0.03) and than healthy participants, who has normal FC levels (p<0.001); the FC levels of patients who did not take NSAIDs also exceeds healthy subject’s levels (p<0.001). The FC levels in the collective group have a sufficient positive correlation with the duration of NSAIDs intake, VAS score and strong correlation with Lequesne index values. We found that both NSAIDs groups have a significantly greater prevalence of elevated or high FC levels than the control group (p<0.001) and that NSAIDs patients significantly more often have high FC levels than those who do not take NSAIDs (p=0.035). When comparing FC levels in patients depending on the type of NSAIDs they take, we found that those who take non-selective NSAIDs has significantly higher FC levels than those who do not take NSAIDs – 264.1 (IQR 89.72-464.67) to 25.65 (IQR 19.5-75.33) (p=0.0003). The FC levels of who take selective NSAIDs – 98.53 (91.56-105.5) – did not differ significantly to non-selective NSAIDs taker’s group values and non-selective NSAIDs taker’s values (p>0.05).Conclusions. Patients who suffer from OA have higher FC levels than healthy individuals, and patients with OA who take NSAIDs regularly have higher FC levels than those who do not. The intake of non-selective NSAIDs is associated with higher FC levels, than the intake of high-selective NSAIDs. FC levels of those who take high-selective NSAIDs do not differ statistically from those who do not intake NSAIDs. Further research is needed in this area.
      PubDate: 2020-03-31
      DOI: 10.21303/2504-5679.2020.001193
       
  • CORRELATION ANALYSIS OF GSTP1 GENE POLYMORPHISM WITH MORBIDITY OF PRIMARY
           METASTATIC COLORECTAL CANCER

    • Authors: Yuri Bazhora, Sergii Chetverikov, Viacheslav Onyshchenko, Mykhailo Chetverikov, Valeriia Chetverikova-Ovchynnyk
      Pages: 25 - 31
      Abstract: Colorectal cancer (CRC) is one of the most common malignancies. Susceptibility to malignant processes is mediated by genetically driven differences in the effectiveness of detoxification of potential carcinogens. One of the factors that may influence the risk of CRC is the glutathione-S-transferase (GST) gene family that encodes glutathione transferase enzymes. The GSTP1 gene is expressed both in normal and pathological conditions. Determining its specific alleles may be a marker of CRC.The aim of the research – to study GSTP1 gene polymorphism, which is likely to be more common among patients with primary metastatic colorectal cancer compared with healthy population.Materials and methods. The study involved 12 patients with primary metastatic colorectal cancer aged 43 to 72 years, the control group was represented by a sample of 31 people without cancer.Results. The incidence of advanced CRC in the presence of GSPT1 Val / Val (aa) polymorphism is statistically significantly higher than in the presence of Ile / Ile (AA) and Ile / Val (Aa) GSPT1 polymorphism.Conclusions. Among people with GSTP1 Val / Val (aa) polymorphism, primary CRC is 4.4 times more likely than among people with GSPT1 Ile / Ile (AA) and Ile / Val (Aa) polymorphisms, which are statistically significant (P < 0.05). The obtained results indicate the possibility of conducting a genetic study of GSTP1 polymorphism to form groups of potential risk of CRC.
      PubDate: 2020-03-31
      DOI: 10.21303/2504-5679.2020.001196
       
  • INFLUENCE OF THE ACUTE INFLAMMATION AND ENDOTHELIAL DYSFUNCTION ON THE
           RETINAL VEIN OCCLUSION FORMATION AFTER CARDIO-SURGICAL INTERFERENCES WITH
           THE USE OF CARDIOPULMONARY BYPASS

    • Authors: Olga Venediktova
      Pages: 32 - 39
      Abstract: Acute inflammation and endothelial dysfunction (EDF) are typical pathological processes, which determine the development of retinal vein occlusion (RVO) during cardio-surgery with the use of cardiopulmonary bypass (CB), but the connection of seromarkers according to the terms of occlusion appearance remains undefined.The aim – to determine the influence of the acute inflammation and EDF for RVO formation after cardio-surgical interferences with the use of CB according to the terms of occlusion appearance.Material and methods. There were selected for the research the data of 137 eyes (126 patients, the main group) with RVO after the surgery with CB. The comparison group contains the data about examination of 86 eyes (43 patients), who had not any occlusion during all term of examination. The control group consisted of 10 eyes (5 patients) without occlusion, which were examined before surgery. An ophthalmologist 2, 7, 30, 60, 90 and 180 days after cardio-surgical interference, examined patients. The content of IL-6, IL-8 and VE-cadherin in blood serum was determined by immunoenzyme technique (Bender Medsystems, Austria). Statistical data processing was performed with the use of Statistica 10 program (StatSoft, Inc., USA), regression analysis – with the use of the program package GLZ.Results. The conduction of cardio-surgeries with the use of CB caused an increase of the interleukins content in the early period (IL-6 on the 2nd and 7th days, and IL-8 up to 30 days), while the content of VE-cadherin (VE-C) was slightly increased during almost all period of monitoring. With the availability of RVO, the content of IL-6 during all terms of occlusion appearance was significantly higher, the content of IL-6 was up to 30 days, and the content of VE-C in a greater degree was after the 7th day.The regression analysis showed that after 1-2 days RVO appearance was directly related with the content of IL-6 and IL-8 in the blood, on the 3rd and 7th days – only with the content of IL-8, on the 8th and 30th days – with the content of all markers, and then with the content of IL-6 and VE-C. The accuracy of the prediction of the presence or absence of RVO at the appropriate period according to the calculated regression model is at least 78 % (p <0.001), what proves the influence of markers on the development of RVO.Conclusions. The undertaken study shows the meaning of the acute inflammation and EDF by appearance of RVO with the use of CB, what justifies the application of the preventive measures - at the early stages the restriction of activity of the inflammatory process, at the later stages – prevention of EDF development.
      PubDate: 2020-03-31
      DOI: 10.21303/2504-5679.2020.001143
       
  • EXPERIMENTAL RATIONALE OF THE USE OF CELL THERAPY FOR THE TREATMENT OF
           GLAUCOMA OPTICAL NEUROPATHY

    • Authors: Sergiy Rykov, Oksana Petrenko, Antonina Yakovets, Dmytro Zubov, Roman Vasyliev
      Pages: 40 - 46
      Abstract: Development of new effective treatments for glaucomatous optic neuropathy is one of the most acute aspects of modern ophthalmology.The aim of the work is to investigate the effectiveness of cell therapy with postnatal multipotent neural crest stem cells (NCSCs) using different cell delivery methods in a model of adrenaline-induced glaucoma.Materials and methods. Glaucoma was induced in Wistar rats by intraperitoneal injections of 10 μg to 15 μg/100 g body weight of 0.18 % adrenaline hydrotartrate. NCSCs were delivered intravenously (5 million cells), retrobulbarly (0.5 million cells) or parabulbarly (0.5 million cells). Histomorphometric analysis of the retina was performed on stained haematoxylin-eosin sections with a thickness of 5 μm one month after the delivery of NCSCs.Results. NCSCs transplantation by all modes of delivery caused positive morphological changes to varying degrees. Intravenous administration induced a decrease in edema in all retinal layers and a slight restoration of the cytoarchitectonics of the retinal layers. The parabulbar administration of NCSCs led to a decrease in edema and the restoration of the cytoarchitectonics of the layers, most pronouncedly the ganglion cell layer and the inner retinal layer. After the retrobulbar administration of NCSCs, the reduction in edema and restoration of the cytoarchitectonics of the layers were the most pronounced.Conclusions. According to the results of the study, the positive effect of NCSCs transplantation in an experimental model of glaucoma was the most pronounced following the retrobulbar injection of cells. Further investigations of the mechanisms of the effect of transplanted NCSCs on retinal structure restoration are needed.
      PubDate: 2020-03-31
      DOI: 10.21303/2504-5679.2020.001187
       
  • PREOPERATIVE ENDOBRONCHIAL SANITATION AS PREPARATION FOR THORACIC
           INTERVENTIONS

    • Authors: Vasiliy Kritsak, Kateryna Ponomarova, Dmitriy Minukhin
      Pages: 46 - 52
      Abstract: The aim – to study the effectiveness of endoscopic photodynamic therapy as a preoperative preparation of the tracheobronchial tree in patients with lung cancer.Materials and methods. The study included 181 patients with II-III stage of lung’s cancer. Diagnose of lung’s cancer was confirmed with a morphologically and concomitant endobronchitis. During the preoperative preparation, we used a technique developed by us introducing into the tracheobronchial tree an aqueous solution of brilliant green at concentration of 0.04 %, followed by irradiation of this solution with laser radiation with the wavelength of 0.63 μm (AFL-2 helium-neon laser) in an independent form and in combination with traditional anti-inflammatory therapy. Endoscopic endobronchial sanitation therapy was carried out by low-intensity radiation of the red part of the spectrum (λ=0.63–0.66 μm) in a pulsed mode, with a power of 12 mW.Results. After 3–5 days of the start preoperative preparation and 1–2 sessions of endoscopic bronchosanation, the general state of patients was improved, the amount of sputum decreased and was changing from mucopurulent to mucous. To completely stop the clinical phenomena of endobronchitis, it was necessary to conduct 5-6 sessions of endoscopic photodynamic therapy according to our methodology, with the introduction of our method. In patients without pronounced clinical symptoms of concomitant chronic bronchitis, it was enough to complete only 3 sessions of bronchosanation.Conclusion. Endoscopic photodynamic bronchosanation in the preoperative period in patients for correcting accompanying endobronchitis can significantly reduce or completely stop the clinical manifestations of endobronchitis in the shortest time and leads to a significant decrease in the number of endobronchial complications in surgical and combined treatment of lung cancer.
      PubDate: 2020-03-31
      DOI: 10.21303/2504-5679.2020.001188
       
  • UTERINE FIBROIDS IMPACT ON FERTILITY AND PREGNANCY OUTCOME

    • Authors: Olena Doroha, Mykola Iarotskyi, Iryna Iarotska, Liudmyla Semeniuk, Olga Nikitina
      Pages: 52 - 58
      Abstract: The clinical confirmation of the relationship between uterine leiomyoma and female infertility is of interest to scholars.The aim of our research was to study obstetric history and the level of sex hormones in women of reproductive age with leiomyoma.Materials and methods. The main group consisted of 90 women of reproductive age with uterine leiomyoma, the control group - 45 healthy women. Gynaecological and obstetric history, hormone content in peripheral blood and pelvic vessels (estradiol, progesterone) were analysed. Statistical processing of clinical material was performed using Microsoft Excel, Statistica 7.0, and Statistica 8.0 for Windows.Results. The frequency of infertility in the surveyed women with LM was 18.9 %. One in three patients in the main group (33.3 %) did not give birth, which was statistically different from the healthy women group. In this case, 25.6 % of women in the main group did not use any methods of contraception. It was found that 51.11 % of patients with LM had threatened miscarriage in their history, 37.5 % – complicated delivery. Pathological changes in the concentrations of estradiol and progesterone in the preferential blood and blood vessels of women with fibroids have been identified. We have found that the content of sex hormones in the local bloodstream has a reliable relationship with the location of the myomatous node.Conclusion. The obtained results may indicate that leiomyoma contributes to the reduction of fertility. This is evidenced by the fact that women with LM are more likely to have the risk of miscarriage, complicated delivery and delivery by caesarean section, including complicated one. The findings have made a significant contribution to the further development of effective strategies for the prevention and treatment of infertility in women with fibrotic uterine lesions.
      PubDate: 2020-03-31
      DOI: 10.21303/2504-5679.2020.001205
       
  • SELECTION OF FLAVOUR ADDITIVES AND METHOD OF THEIR INTRODUCTION IN THE
           COMPOSITION OF COMPRESSED MEDICATED CHEWING GUMS

    • Authors: Yuliia Maslii, Оlena Ruban, Sergiy Kutsenko
      Pages: 59 - 66
      Abstract: An important characteristic of oral medicines, which include medicated chewing gums, is their pleasant taste. This can be achieved by adding taste coregents to their composition.The aim: to choose rational flavour additives and justify the method of their introduction to the compressed medicated chewing gums that are being developed.Materials and methods: mint (Natural Mint Flavor SD, Kerry Inc., Malaysia), apple (Nat Apple FlavorWonf, Kerry Inc., Malaysia) and banana (Banana FLV, Kerry Inc., Malaysia) have been used as powdered flavour additives. As flavourings – oil solutions: Peppermint Natural WonfFlavor, Kerry Inc., Malaysia), Apple (Nat Apple FlavorWonf, Kerry Inc., Malaysia), Strawberry (Strawberry FlavorWonf, Kerry Inc., Malaysia), Melon (Chemical-Food Aromatic PlantLLC, Russian Federation) and Lemon (Chemical-Food Aromatic Plant LLC, Russian Federation). Aerosil brand 380 (Evonik Resource Efficiency GmbH, Germany), Syloid® 244FP (Grace Discovery Sciences, USA) and Neusilin® ULP 2 (Fuji Chemical Industry Co. Ltd, Japan) have been taken as carriers. The choice of flavour additives and flavourings was made using organoleptic methods of coregents evaluation according to A. I. Tentsova and I. A.Yegorov. The technological and physicochemical properties of the samples were studied according to conventional methods of the State Pharmacopoeia of Ukraine.Results and discussion. In determining the taste of medicated chewing gums samples with different coregents, the combination of powder additive and liquid flavouring "Apple" has got the highest mark. In order to substantiate the rational method of liquid flavouring introduction in the composition of the compressed chewing gums, it is proposed to introduce the adsorbents Aerosil 380, Syloid® 244FP and Neusilin® ULP 2, which also play the role of moisture regulators and glidants. Microscopic analysis of adsorbents mixtures with flavouring, as well as physicochemical and technological investigations of the mass for pressing have revealed that the best adsorbing, moisture-regulating and flow properties has Syloid® 244FP.Conclusions. As a coregent in medicated chewing gums was selected a combination of powdered (2.0 %) and liquid (0.6 %) flavours “Apple”. The method of introducing the oil flavouring into the composition of the compressed gums has been chosen – by spraying on the adsorbent with subsequent mixing. Based on the studies, Syloid®244FP at a concentration of 1.0 % was chosen as the rational carrier.
      PubDate: 2020-03-31
      DOI: 10.21303/2504-5679.2020.001189
       
  • RESEARCH ON THE SELECTION OF EXCIPIENTS AS THE RATIONALE FOR THE
           COMPOSITION OF THE TABLETS WITH DRY EXTRACT OF SANGUISORBA OFFICINALIS

    • Authors: Lyudmila Shulga, Kateryna Bezkrovna, Nina Domar
      Pages: 66 - 75
      Abstract: The aim. The aim of the research was to study the effect of different groups of excipients on the pharmaco-technological propertiesof the powder mass for tabletting in the development of the composition of the tablets with dry extract of Sanguisorba officinalis for complex therapy of the gastrointestinal tract diseases.Materials and methods. Objects of study - dry extract Sanguisorba officinalis, 25 excipients used in the production of tablets by the method of direct compression, grouped into five groups of factors (fillers based on sugars and microcrystalline cellulose, disintegrants, glidants and lubricants), samples of powder masses. Studies on the determination of pharmaco-technological properties (fluidity, bulk density, bulk density after shrinkage, degree of compressibility, Hausner ratio, and angle of repose) of the obtained powder masses were carried out according to the methods of the State Pharmacopoeia of Ukraine, Second edition. The method of mathematical planning of the experiment was used in the work, the obtained results were subjected to variance analysis, and the ranked series of advantages were placed, in which the excipients were placed in the sequence of their influence on the studied pharmaco-tecnological parameters.Results and discussion. The influence of excipients (factors) on the pharmaco-technological properties (responses) of the powdered tablet masses with the construction of ranked benefits was studied using a five-factor experiment, a hyper-Graeco-Latin square. The results of the analysis of variance showed that glidants have the greatest influence on the fluidity, the bulk density and the bulk density after shrinkage. Neusilin US 2 significantly affects the fluidity of the powder masses and Hausner ratio, the talc having the greatest effect on the bulk density and the bulk density after shrinkage of the powder masses. The representative of the disintegrants group – Sodium starch glycolate most influences the compressibility index, the sugar-based filler – Pearlitol 500 DC – on the angle of repose.Conclusions. The effect of 25 excipients on the pharmaco-technological characteristics of the powdered tablet masses with dry extract of Sanguisorba officinalis was studied. It was found that among the sugars-based fillers equally good results were shown in the powder masses with Compri sugar, Tablettose 80 and Pearlitol 500 DC; among the microcrystalline cellulose based fillers is Prosolv 90; among glidants – Neusilin US 2; no comparison was made of the disintegrants and lubricant excipients from the studied list of leader substances. The results of the studies indicate the possibility of obtaining tablets by the direct compression method, and further study of their pharmaco-technological characteristics will allow to establish the optimal composition of excipients.
      PubDate: 2020-03-31
      DOI: 10.21303/2504-5679.2020.001195
       
  • PATHOMORPHOLOGICAL CHANGES IN THE SKIN OF THE GUINEA PIGS IN THE COURSE OF
           MICROSPORES

    • Authors: Iaroslav Kisera, Yulya Martyniv, Ivan Klishch
      Pages: 76 - 84
      Abstract: During the course of microspores on the skin, the balance between the species of saprophytic microorganisms and hypersensitivity as an integral part of the inflammatory reaction resulting from the presence of the pathogen in the skin is disturbed. The degree of expression of inflammation varies. Clinical manifestations of microsporia are manifested by erythema, alopecia, scaly and crust formation.The aim. Histological studies were performed to determine pathomorphological changes in the skin of guinea pigs during microsporia. The studies were conducted on clinically healthy and sick guinea pigs during the acute course of the disease, during regeneration and during recovery.And also carry out a description and characterization of the identified changes in the skin.The obtained research results showed that patients with microsporia had changes in the morphological composition of the skin, which were characterized by thickening and keratinization of the epidermis, there were small-focal polymorphic cell infiltrates, there was a violation of the microvasculature and the formation of micellar formations.Materials and methods. The material for histological examination(pieces of guinea pig skin) was fixed in a 10–12 % cooled solution of neutral formalin, followed by pouring into paraffin according to the scheme proposed by G.A. Merkulov (1969). Histomic sections 5–7 μm thick were made on the MC-2 dome microtome. Hematoxylin and eosin staining were used for the morphological evaluation of cells and tissue. Microscopy was performed using an OLIMPUS CX-41 microscope.Results. Signs of superficial inflammation with hyperkeratosis and desquamation of cells of the stratified epithelium, microcracks of the epidermis were found in the skin of the patient with microsporia of the tentacle during the acute course. Signs of inflammation of the follicles and surrounding tissues are visualized. Round-cell and plasma connective tissue infiltrates of the connective tissue of the skin were detected by lymphoid, histiocytic and other cellular elements. There is moderate leukocyte infiltration in the dermis. Signs of inflammation by type of infiltration around the hair follicles, enlargement of the blood capillaries and the presence of microabscesses.In the stage of regeneration during recovery, a clear contoured layer of epithelial cells is established. There are no signs of inflammatory reaction in the dermis.Conclusion. In microspores, inflammatory processes occur in all layers of the skin. The skin of sick animals loses its barrier function due to pathomorphological changes. The long-term consequences of the pathological process during the course of microspores can provoke the development of chronic dermatitis with scleroderma.During the course of microspores on the skin, the balance between the species of saprophytic microorganisms and hypersensitivity as an integral part of the inflammatory reaction resulting from the presence of the pathogen in the skin is disturbed. The degree of expression of inflammation varies. Clinical manifestations of microsporia are manifested by erythema, alopecia, scaly and crust formation.The aim. Histological studies were performed to determine pathomorphological changes in the skin of guinea pigs during microsporia. The studies were conducted on clinically healthy and sick guinea pigs during the acute course of the disease, during regeneration and during recovery.And also carry out a description and characterization of the identified changes in the skin.The obtained research results showed that patients with microsporia had changes in the morphological composition of the skin, which were characterized by thickening and keratinization of the epidermis, there were small-focal polymorphic cell infiltrates, there was a violation of the microvasculature and the formation of micellar formations.Materials and methods. The material for histological examination(pieces of guinea pig skin) was fixed in a 10–12 % cooled solution of neutral formalin, followed by pouring into paraffin a...
      PubDate: 2020-03-31
      DOI: 10.21303/2504-5679.2020.001124
       
 
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