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Polish Journal of Microbiology
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  This is an Open Access Journal Open Access journal
ISSN (Print) 1733-1331 - ISSN (Online) 2544-4646
Published by Exeley Inc Homepage  [17 journals]
  • Pol J Microbiol 2017; 66(2):181-188 "Effect of Lyophilization on
           Survivability and Growth Kinetic of Trichoderma Strains Preserved on
           Various Agriculture By-Products"

    • Authors: Danuta Witkowska; Katarzyna Buska-Pisarek, Wojciech Łaba, Michał Piegza, Anna Kancelista
      Abstract: Growth of four [i]Trichoderma[/i] strains were tested on lignocellulosic by-products in solid state fermentation (SSF). The strains were also analyzed for their survival rate and growth after lyophilization on these carriers. All applied monocomponent and bicomponent media were substrates for the production and preservation of [i]Trichoderma[/i] biomass. However, the maximum number of colony forming units (CFU/g dm) was acquired on bicomponent media based on dried grass and beet pulp or grass with corn cobs, when compared to monocomponent media. Although the process of lyophilization reduced the survival rate by 50%-60%, the actual number of viable cells in obtained biopreparations remained relatively high (0.58 × 108-1.68 × 108 CFU/g dm). The studied strains in the preserved biopreparations were characterized by a high growth rate, as evaluated in microcultures using the Bioscreen C system.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):189-199 "Gene Expression during BTEX
           Biodegradation by a Microbial Consortium Acclimatized to Unleaded Gasoline
           and a Pseudomonas putida Strain (HM346961) Isolated from It"

    • Authors: Jesus A. Morlett-Chavez ; Jose A. Ascacio-Martinez, William E. Haskins , Karim Acuña-Askar, Hugo A. Barrera-Saldaña
      Abstract: [i]Pseudomonas putida[/i] strain (HM346961) was isolated from a consortium of bacteria acclimatized to unleaded gasoline-contaminated water. The consortium can efficiently remove benzene, toluene, ethylbenzene and xylene (BTEX) isomers, and a similar capability was observed with the [i]P. putida[/i] strain. Proteome of this strain showed certain similarities with that of other strains exposed to the hydrocarbon compounds. Furthermore, the toluene di-oxygenase ([i]tod[/i]) gene was up-regulated in [i]P. putida[/i] strain when exposed to toluene, ethylbenzene, xylene, and BTEX. In contrast, the tod gene of[i] P. putida[/i] F1 (ATCC 700007) was up-regulated only in the presence of toluene and BTEX. Several differences in the nucleotide and protein sequences of these two tod genes were observed. This suggests that tod up-regulation in [i]P. putida [/i]strain may partially explain their great capacity to remove aromatic compounds, relative to[i] P. putida[/i] F1. Therefore, new [i]tod [/i]and [i]P. putida[/i] strain are promising for various environmental applications.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):201-207 "The Diversity, Growth Promoting
           Abilities and Anti-microbial Activities of Bacteria Isolated from the
           Fruiting Body of Agaricus bisporus"

    • Authors: Quanju Xiang; Lihua Luo, Yuhuan Liang, Qiang Chen, Xiaoping Zhang, Yunfu Gu
      Abstract: [i]Agaricus bisporus[/i] plays an important role in ecological processes and is one of the most widely cultivated mushrooms worldwide. Mushroom growth-promoting bacteria have been isolated from casing soil and compost, but microorganisms in the fruiting body have received only a little attention. To get an overview of phylogenetic diversity of microorganisms in the fruiting body of [i]A. bisporus[/i], as well as to screen antimicrobial and mushroom growth-promoting strains, and eventually intensify mushroom production, we isolated and characterized microorganisms from the fruiting body of A. bisporus. In total, 55 bacterial strains were isolated, among which nine isolates represented Actinomycetes. All the isolates were analyzed by 16S rRNA gene RFLP and sixteen representative strains by 16S rRNA gene sequencing. According to the phylogenetic analysis, eleven isolates represented the Gram-positive [i]Bacillus[/i], [i]Lysinibacillus[/i], [i]Paenibacillus[/i], [i]Pandorea [/i]and [i]Streptomyces [/i]genera, and five isolates belonged to the Gram-negative [i]Alcaligenes [/i]and [i]Pseudomonas [/i]genera. The bacteria isolated from the fruiting body of [i]A. bisporus[/i] had broad-spectrum antimicrobial activities and potential mushroom growth-promoting abilities.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):209-221 "Molecular Study of Indigenous
           Bacterial Community Composition on Exposure to Soil Arsenic Concentration

    • Authors: Semanti Basu; Tanima Paul, Priya Yadav, Abhijit Debnath, Keka Sarkar
      Abstract: Community structure of bacteria present in arsenic contaminated agricultural soil was studied with qPCR (quantitative PCR) and DGGE (Denaturing Gradient Gel Electrophoresis) as an indicator of extreme stresses. Copy number of six common bacterial taxa ([i]Acidobacteria[/i], [i]Actinobacteria[/i], [i]α[/i]-, [i]β[/i]- and [i]γ[/i]-[i]Proteobacteria[/i], [i]Firmicutes[/i]) was calculated using group specific primers of 16S rDNA. It revealed that soil contaminated with low concentration of arsenic was dominated by both [i]Actinobacteria [/i]and [i]Proteobacteria [/i]but a shift towards [i]Proteobacteria [/i]was observed with increasing arsenic concentration, and number of [i]Actinobacteria [/i]eventually decreases. PCA (Principle Component Analysis) plot of bacterial community composition indicated a distinct resemblance among high arsenic content samples, while low arsenic content samples remained separated from others. Cluster analysis of soil parameters identifies three clusters, each of them was related to the arsenic content. Further, cluster analysis of 16S rDNA based DGGE fingerprint markedly distributed the soil bacterial populations into low (< 10 ppm) and high (> 10 ppm) arsenic content subgroups. Following analysis of diversity indices shows significant variation in bacterial community structure. MDS (Multi Dimensional Scaling) plot revealed distinction in the distribution of each sample denoting variation in bacterial diversity. Phylogenetic sequence analysis of fragments excised from DGGE gel revealed the presence of [i]γ[/i]-[i]Proteobacteria [/i]group across the study sites. Collectively, our experiments indicated that gradient of arsenic contamination affected the shape of the soil bacterial population by significant structural shift.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):223-233 "Safety Evaluation of Enterocin
           Producer Enterococcus sp. Strains Isolated from Traditional Turkish

    • Authors: Mine Avcı; Banu Özden Tuncer
      Abstract: The purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in [i]Enterococcus [/i]spp. isolated from different cheeses and also investigate some of their virulence factors. [i]Enterococcus [/i]strains were isolated from 33 different cheeses. [i]Enterococcus faecium[/i] (6 strains) and [i]Enterococcus faecalis[/i] (5 strains) enterocin-producing strains were identified by 16S rDNA analyses. Structural genes [i]ent[/i]A, [i]ent[/i]B, [i]ent[/i]P and [i]ent[/i]X were detected in some isolates. Multiple enterocin structural genes were found in 7 strains. None of the tested enterococci demonstrated anyβ-haemolytic activity and only one strain had gelatinase activity. Six strains showed multiple antibiotic resistance patterns and in addition, vanA and several virulence genes were detected in many strains. Only [i]E. faecalis[/i] MBE1-9 showed tyrosine decarboxylase activity and tdc gene was detected only in this strain.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):245-250 "In vitro Interaction between
           Fumonisin B1 and the Intestinal Microflora of Pigs"

    • Authors: Huu Anh Dang; Attila Zsolnai, Melinda Kovacs, Istvan Bors, Andras Bonai, Brigitta Bota, Judit Szabo_Fodor
      Abstract: The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B1 (5 μg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for enumerating the amount of bacteria including aerobic, anaerobic bacteria, coliform, [i]Escherichia coli[/i] and [i]Lactobacillus[/i] sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, [i]Lactobacillus[/i], [i]Bacteroides [/i]and [i]Prevotella[/i]. No significant differences in the amount of bacterial groups between the experimental (buffer, chyme, and fumonisin B[sub]1[/sub]) and control 1 groups (buffer + chyme) were observed in both methods. Fumonisin B[sub]1[/sub] and hydrolysed fumonisin B[sub]1[/sub] concentration were analysed by liquid chromatograghy – mass spectrometry. There was no significant difference in FB[sub]1[/sub] concentration between the experimental and the control 2 group (buffer and fumonisin B[sub]1[/sub]) at 0 h incubation, 5.185 ± 0.174 μg/ml compared with 6.433 ± 0.076 μg/ml. Fumonisin B[sub]1[/sub] concentration in the experimental group was reduced to 4.080 ± 0.065 μg/ml at 24 h and to 2.747 ± 0.548 μg/ml at 48 h incubation and was significantly less than that of in the control group. Hydrolysed fumonisin B[sub]1[/sub] was detected after 24 h incubation (0.012 ± 0 μg/ml). At 48 h incubation time, hydrolysed fumonisin B[sub]1[/sub] concentration was doubled to 0.024 ± 0.004 μg/ml. These results indicate that fumonisin B[sub]1[/sub] can be metabolised by caecal microbiota in pigs though the number of studied bacteria did not change.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):251-254 "Early KPC-Producing Klebsiella
           pneumoniae Bacteremia among Intensive Care Unit Patients Non-Colonized
           upon Admission"

    • Authors: Matthaios Papadimitriou-Olivgeris; Fotini Fligou, Iris Spiliopoulou, Christina Bartzavali, Vasiliki Dodou, Sophia Vamvakopoulou, Kyriaki Koutsileou, Anastasia Zotou, Evangelos D. Anastassiou, Myrto Christofidou, Markos Marangos
      Abstract: Among 140 patients colonized by KPC-producing [i]Klebsiella pneumoniae[/i] (KPC-Kp) between fourth and seventh day of Intensive Care Unit stay, 24 developed bacteraemia immediately after colonization. Colistin-resistance of the colonizing isolate was the factor significantly associated with early KPC-Kp bacteraemia (P < 0.001; OR 6.6, 95% CI 2.4–18.4), a worrisome finding since infections by colistin-resistant isolates is associated with increased mortality due to limited remaining therapeutic options.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):255-257 "An Improve Protocol for PCR Using LM1
           and LM2 Primers for Listeria monocytogenes Detection in Food Matrices"

    • Authors: Angélica Godínez-Oviedo; Gerardo M. Nava, Sofía M. Arvizu-Medrano, Montserrat Hernández-Iturriaga
      Abstract: Several studies have observed that a conventional PCR protocol using primers LM1 and LM2 for the identification of gene[i] hly[/i]A [i]Listeria monocytogenes[/i] generates non-specific PCR amplifications and false positives. For this reason in this study, we provide a modified PCR protocol that improves the specificity of the LM1 and LM2 primers
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):259-263 "Bactericidal Activity of Octenidine
           to Various Genospecies of Borrelia burgdorferi, Sensu Lato Spirochetes in
           Vitro and in Vivo"

    • Authors: Stanisława Tylewska-Wierzbanowska; Urszula Rogulska, Grażyna Lewandowska, Tomasz Chmielewski
      Abstract: The aim of our studies was to invent a reliable method for detection of the bactericidal activity of disinfectants against [i]Borrelia burgdorferi[/i] in suspension ([i]in vitro[/i]) and in cell line cultures ([i]in vivo[/i]). In the suspension method, 0.01% octenidine at 20°C and 35°C was bactericidal to [i]Borrelia afzeli[/i]; [i]Borrelia garini[/i], [i]B. burgdorferi[/i] sensu stricto after 5 minutes treatment. Increase of the temperature to 35°C speed up the bactericidal effect to 1 minute. The bactericidal action of octenidine towards [i]B. burgdorferi [/i]spirochetes growing in fibroblasts was less effective and needed a longer time to kill them than in the suspension.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):265-268 "Erythromycin or Clindamycin – is it
           Still an Empirical Therapy against Streptococcus agalactiae in Patients
           Allergic to Penicillin'"

    • Authors: Piotr Leszczyński; Beata Sokół-Leszczyńska, Bronisława Pietrzak, Anna Sawicka-Grzelak, Mirosław Wielgoś
      Abstract: Retrospective analysis of [i]Streptococcus agalactiae[/i] antibiotic susceptibility isolated in 2010–2013 was performed. Penicillin was still the first-line antibiotic. Due to the high percentage of strains resistant to erythromycin and clindamycin empirical treatment with these antibiotics may not be effective. Lower resistance rate to erythromycin and clindamycin among strains isolated from infected pregnant women and newborns were observed than among strains isolated from samples from patients hospitalized in other departments (29% and 47% [i]v.[/i] 46% and 63%). The increasing resistance rate might give a rise to a new epidemiological situation.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):269-272 "Influence of Pseudomonas and Bacillus
           Strains Isolated from Lolium perenne Rhizospheric Soil in Vojvodina
           (Serbia) on Planth Growth and Soil Microbial Communities"

    • Authors: Stamenov R. Dragana; Simonida Đurić, Timea I Hajnal Jafari, Snezana Anđelković
      Abstract: The aim of this study was the isolation of [i]Pseudomonas [/i]sp. and [i]Bacillus [/i]sp. strains from rhizospheric soil and monitoring the impact of two isolates denoted as P12 ([i]Pseudomonas [/i]sp.) and B1 ([i]Bacillus [/i]sp.) on the parameters of English ryegrass ([i]Lolium perenne[/i]) yield and activity of the soil microbial communities. During 2012–2014, a plot experiment was set up following the randomized block system. Better effect on the plant growth was recorded with the use of [i]Pseudomonas [/i]sp. P12 isolate than with [i]Bacillus [/i]sp. B1. Positive effect on the increase in the total number of microorganisms, aminoheterotrophs and azotobacter was also achieved. [i]Bacillus [/i]sp. B1 increased only the number of actimycetes. Both isolates positively affected dehydrogenase activity (DHA).
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):277-279 "The Serological Surveillance of
           Hepatitis E virus among Hunters and Foresters in Eastern Poland"

    • Authors: Marcin Weiner; Małgorzata Tokarska-Rodak, Dorota Plewik, Anna Pańczuk, Adam Szepeluk, Monika Krajewska
      Abstract: The aim of the study was the analysis of the occurrence of specific antibodies against HEV among hunters and foresters who are at risk to be exposed. The study group consisted of 210 hunters (23–80 years old) and 60 foresters (22–64 years old). Anti-HEV IgG were present in 3.81% of the samples of the hunters and in 5% of the samples of the foresters. The statistical analysis shows no significant differences in the results anti-HEV IgG between the groups of hunters and foresters (p = 0.5278). Significantly higher anti-HEV IgG titers were found in the older age group (> 55 years old).
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):281-285 "Morphological and Molecular
           Characterization of Phoma complanata,a New Causal Agent of Archangelica
           officinalis Hoffm. in Poland"

    • Authors: Beata Zimowska; Ewa D. Zalewska, Ewa D. Król, Agnieszka Furmańczyk
      Abstract: The paper concerns the fungus [i]Phoma complanata[/i], isolated for the first time in Poland, from the roots and umbels of angelica ([i]Archangelica officinalis[/i]) in 2009. The morphology of fungal isolates was tested on standard culture media. Moreover, the sequence analysis of ITS regions was conducted. Morphological similarity of [i]P. complanata [/i]Polish isolates to the reference isolate obtained from CBS culture collection was determined and together with the molecular analysis confirmed the affiliation of the fungus to the species.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):151-161 "Biochar–rhizosphere
           interactions – A review"

    • Authors: Sławomir Głuszek; Lidia Sas Paszt, Beata Sumorok, Ryszard Kozera
      Abstract: Biochar is a solid material of biological origin obtained from the biomass carbonization, designed as a mean to reduce greenhouse gases emission and carbon sequestration in soils for a long time. Biochar has a wide spectrum of practical utilization and is applied as a promising soil improver or fertilizer in agriculture, or as a medium for soil or water remediation. Preparations of biochar increase plant growth and yielding when applied into soil and also improve plant growth conditions, mainly bio, physical and chemical properties of soil. Its physical and chemical properties have influence on bacteria, fungi and invertebrates, both in field and laboratory conditions. Such effects on rhizosphere organisms are positive or negative depending on biochar raw material origin, charring conditions, frequency of applications, applications method and doses, but long terms effects are generally positive and are associated mainly with increased soil biota activity. However, a risk assessment of biochar applications is necessary to protect the food production and soil environment. This should be accomplished by biochar production and characterization, land use implementation, economic analysis, including life cycle assessment, and environmental impact assessment.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):163-169 "Genetic Characterization of a Novel
           Composite Transposon Carrying armA and aac(6)-Ib Genes in an Escherichia
           coli Isolate from Egypt"

    • Authors: Mona T. Kashef; Omneya M. Helmy
      Abstract: Aminoglycosides are used in treating a wide range of infections caused by Gram-positive and Gram-negative bacteria; however, aminoglycoside resistance is common and occurs by several mechanisms. Among these mechanisms is bacterial rRNA methylation by the 16S rRNA methyl transferase (16S-RMTase) enzymes; but data about the spread of this mechanism in Egypt are scarce. Cephalosporins are the most commonly used antimicrobial agents in Egypt; therefore, this study was conducted to determine the frequency of 16S-RMTase among third generation cephalosporin-resistant clinical isolates in Egypt. One hundred and twenty three cephalosporin resistant Gram-negative clinical isolates were screened for aminoglycosides resistance by the Kirby Bauer disk diffusion method and tested for possible production of 16S-RMTase. PCR testing and sequencing were used to confirm the presence of 16S-RMTase and the associated antimicrobial resistance determinants, as well as the genetic region surrounding the armA gene. Out of 123 isolates, 66 (53.66%) were resistant to at least one aminoglycoside antibiotic. Only one [i]Escherichia coli[/i] isolate (E9ECMO) which was totally resistant to all tested aminoglycosides, was confirmed to have the armA gene in association with [i]bla[/i][sub]TEM-1[/sub], [i]bla[/i][sub]CTX-M-15[/sub], [i]bla[/i][sub]CTX-M-14[/sub] and [i]aac(6)-Ib[/i] genes. The [i]arm[/i]A gene was found to be carried on a large A/C plasmid. Genetic mapping of the armA surrounding region revealed, for the first time, the association of [i]arm[/i]A with [i]aac(6)-Ib[/i] on the same transposon. In conclusion, the isolation frequency of 16S-RMTase was low among the tested aminoglycoside-resistant clinical samples. However, a novel composite transposon has been detected conferring high-level aminoglycosides resistance.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):171-180 "Trends of Bloodstream Infections in a
           University Greek Hospital during a Three-Year Period: Incidence of
           Multidrug-Resistant Bacteria and Seasonality in Gram-negative Predominance

    • Authors: Fevronia Kolonitsiou; Matthaios Papadimitriou-Olivgeris, Anastasia Spiliopoulou, Vasiliki Stamouli, Vasileios Papakostas, Eleni Apostolopoulou, Christos Panagiotopoulos, Markos Marangos, Evangelos D. Anastassiou, Myrto Christofidou, Iris Spiliopoulou
      Abstract: The aim of the study was to assess the epidemiology, the incidence of multidrug-resistant bacteria and bloodstream infections’ (BSIs) seasonality in a university hospital. This retrospective study was carried out in the University General Hospital of Patras, Greece, during 2011–13 y. Blood cultures from patients with clinical presentation suggestive of bloodstream infection were performed by the BacT/ALERT System. Isolates were identified by Vitek 2 Advanced Expert System. Antibiotic susceptibility testing was performed by the disk diffusion method and E-test. Resistance genes ([i]mec[/i]A in staphylococci;[i] van[/i]A/[i]van[/i]B/[i]van[/i]C in enterococci; [i]bla[/i][sub]KPC[/sub]/[i]bla[/i][sub]VIM[/sub]/[i]bla[/i][sub]NDM[/sub] in [i]Klebsiella[/i] spp.) were detected by PCR. In total, 4607 (9.7%) blood cultures were positive from 47451 sets sent to Department of Microbiology, representing 1732 BSIs. Gram-negative bacteria (52.3%) were the most commonly isolated, followed by Gram-positive (39.5%), fungi (6.6%) and anaerobes bacteria (1.8%). The highest contamination rate was observed among Gram-positive bacteria (42.3%). Among 330 CNS and 150 [i]Staphylococcus aureus[/i], 281 (85.2%) and 60 (40.0%) were [i]mec[/i]A-positive, respectively. From 113 enterococci, eight were [i]van[/i]A, two [i]van[/i]B and two [i]van[/i]C-positives. Of the total 207 carbapenem-resistant Klebsiella pneumoniae (73.4%), 202 carried [i]bla[/i][sub]KPC[/sub], four [i]bla[/i][sub]KPC[/sub] and [i]bla[/i][sub]VIM[/sub] and one [i]bla[/i][sub]VIM[/sub]. A significant increase in monthly BSIs’ incidence was shown (R2: 0.449), which may be attributed to a rise of Gram-positive BSIs (R2: 0.337). Gram-positive BSIs were less frequent in spring (P < 0.001), summer (P < 0.001), and autumn (P < 0.001), as compared to winter months, while Gram-negative bacteria (P < 0.001) and fungi (P < 0.001) were more frequent in summer months. BSIs due to methicillin resistant S. aureus and carbapenem-resistant Gram-negative bacteria increased during the study period. The increasing incidence of BSIs can be attributed to an increase of Gram-positive BSI incidence, even though Gram-negative bacteria remained the predominant ones. Seasonality may play a role in the predominance of Gram-negative’s BSI.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):235-243 "Intracellular Siderophore Detection
           in an Egyptian, Cobalt-Treated F. solani Isolate Using SEM-EDX with
           Reference to its Tolerance"

    • Authors: Rasha M. Farrag
      Abstract: An Egyptian, plant pathogenic [i]Fusarium solani[/i] isolate was grown on cobalt concentrations of 0, 50, 200, 500, 800, and 1000 ppm. The isolate survived concentrations up to 800 ppm, however failed to grow at 1000 ppm. Morphology and elemental analysis of the isolate under the investigated Co concentrations were studied using Scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). The isolate reserved its morphology up to a concentration of 200 ppm. Morphological distortions were dramatic at 500 and 800 ppm. EDX detected Co uptake through the hyphae, microconidia, macroconidia, and chlamydospores. Iron, calcium, and potassium were also detected. EDX results showed a linear relationship between Co% and Fe% up to a concentration of 500 ppm reflecting the possible ability of the isolate to synthesize intracellular siderophores storing iron and their release from the vacuoles. The participation of such siderophores in conferring tolerance against cobalt is discussed. At 800 ppm, the % of Fe was greatly reduced with an accompanying increase in morphological distortions and absence of microconidia. Increasing the implicated cobalt concentrations resulted in increasing the percentages of the chelated cobalt reflecting the possible implication of the cell wall as well as extracellular siderophores in the uptake of cobalt. The current results recommend the absence of cobalt in any control regime taken to combat the investigated [i]F. solani[/i] isolate and highlights the accomplishment of biochemical, ultrastructural, and molecular studies on such isolate to approve the production of siderophores and the role of cell wall in cobalt uptake.
      PubDate: Thu, 6 Jul 2017 9:57 EST
  • Pol J Microbiol 2017; 66(2):273-276 "Betaine Improves Polymer-Grade
           D-Lactic Acid Production by Sporolactobacillus inulinus Using Ammonia as
           Green Neutralizer"

    • Authors: Guoping Lv; Chengchuan Che, Li Li, Shujing Xu, Wanyi Guan, Baohua Zhao, Jiansong Ju
      Abstract: The traditional CaCO3-based fermentation process generates huge amount of insoluble CaSO4 waste. To solve this problem, we have developed an efficient and green D-lactic acid fermentation process by using ammonia as neutralizer. The 106.7 g/L of D-lactic acid production and 0.89 g per g of consumed sugar were obtained by Sporolactobacillus inulinus CASD with a high optical purity of 99.7% by adding 100 mg/L betaine in the simple batch fermentation process. The addition of betaine was experimentally proven to protect cell at high concentration of ammonium ion, increase the D-lactate dehydrogenase specific activity and thus promote the production of D-lactic acid.
      PubDate: Thu, 6 Jul 2017 9:57 EST
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
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