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Kocatepe Veteriner Dergisi
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  This is an Open Access Journal Open Access journal
ISSN (Print) 1308-1594 - ISSN (Online) 2147-6853
Published by Afyon Kocatepe Üniversitesi Homepage  [5 journals]
  • Some Andrological Parameters and Biochemical Properties in Relation to
           Season in Rams

    • Authors: Deniz YENİ; Mustafa GÜNDOĞAN
      Abstract: In this study averagely 18-20 months aged total ten head rams were usedto evaluate the testicle morphometric measurements, semen characteristics,spermatozoon DNA damage, some blood biochemical properties and blood hormonallevels were monthly assessed together with climatic data. While themorphometric testes measurements of the rams were greater (p<0.05) in theperiod between August and October than other months, the lowest values wereobtained in the period between January and April. The highest values of volume,mass activity, motility and density levels, as to the spermatological featureswere found in the period between August and October and the highest values ofthe plasma membrane integrity just were found in November (p<0.05).Nevertheless, abnormal and dead sperm ratios were found to be significantlylower (p<0.05) in September and October than other months. The lowest DNAdamage was determined on September in semen. Blood testosterone levels werefound greater September and October than the other months. The lowest values oftriiodothyronine levels were obtained in August, November and December(p<0.05). While total protein, albumin and globulin levels were found to besignificantly high (p<0.05) in November, the lowest triglyceride level wasobtained in November. Nevertheless, the positively correlation among sperm DNAdamage, sperm volume (r: 0.18) andabnormal sperm ratio (r: 0.30), thenegatively correlation between sperm DNA damage and semen viscosity (r: -0.32)(p<0.01) were found to be significant. In conclusion, morphometric testesmeasurements showed that semen characteristics, spermatozoon DNA damage,certain blood sera biochemical properties and hormonal levels are affected bythe season at a significant level. Optimum values are obtained in breedingseason which started from the second half of August until mid November and thecorrelation between findings of morphometric testes measurements andspermatological features can be used for selection of stud animals.
      PubDate: Thu, 01 Mar 2018 00:00:00 +030
       
  • A Light Microscopic Study: Conjunctiva-Associated Lymphoid Tissue in Camel

    • Authors: Ahmet CEYLAN
      Abstract: Conjunctiva-associated lymphoid tissue (CALT)is one of the most important accessory component of the eye. This study wasundertaken to demostrate the histological distribution and characteristicfeatures of CALT in healhty camels using light microscopy technique. A totalupper and lower eyelids of 5 (age range, 13-19) camels were investigated. Thegross appearance of CALT were revealed by acetic acid application. Fully intactsolitary and aggregated lymphoid follicles were observed as members of CALT inall camels. These follicles were covered by a thin, flattened epiteheliumcalled follicular-associated epithelium (FAE) that contained intra epitheliallymphocytes and lacked goblet cells. In addition germinal centers, corona,subepithelial dome region and interfolliculer areas were noticed within thelymphoid follicles. The presence of high endothelial venules (HEV), a highlydistinctive feature of CALT, was confirmed in interfollicular areas. Weconclude that CALT of camel closely resembles other mucosal lymphoid tissues andmay serve as an important member of ocular defense mechanism with itsdeterminative features.
      PubDate: Thu, 01 Mar 2018 00:00:00 +030
       
  • Comparison of two Hematocrite Measurement Methods in Diarrheic Neonatal
           Calves

    • Authors: Hasan ERDOĞAN; Mehmet GÜLTEKİN, Serdar PAŞA, Kerem URAL
      Abstract: Inthis study, it was aimed to compare two different hematocrit measurement techniqueswhich are commonly used in veterinary medicine by cell count, and amperometricmethod. A total of 84 neonataldiarrheic calves from both sexes with different etiologies were included in thestudy. For hematocrit measurements, blood samples (4 ml) were taken to bloodgas injectors and tubes containing lithium heparin from calves. The AbacusJunior Vet 5 whole blood cell counter and Irma True Point blood gas analyzerwere used for hematocrit measurements. Accordingto the results of linear regression analysis and Pearson correlation, it wasdetermined that the data obtained from the hematocrit measurements had positivecorrelations between r = 0.90 (p <0.000) between the two measurementtechniques, but statistically significant differences were found between themean values of the two test techniques. In Passing-bablok analysis y= 0.88x+1.81 equation was found. According to the results, both measurements can be utilized inassessing hematocrit measurement, but in case of the difference between the twomeasuring methods, it was concluded that suspected cases should be made basedon the conventional measurement microhematocrit centrifugation.
      PubDate: Thu, 01 Mar 2018 00:00:00 +030
       
  • Estimation of Volume of Ox Brain and Gray and White Matter with
           Cavalier’s Principle

    • Authors: Durmuş BOLAT
      Abstract: Weestimated the volume values, ratios, and the relationship between brain weight andvolumetric values ​​of gray and white matter of the ox brain. Six fresh brainsof Holstein-Friesian strain healthy ox (17-20 months old) were used. Freshweights of the brains were measured using digital weighing. Brains were fixedby immersion method in 10% formalin solution for 3 weeks before measurements ofgray and white matter volumes using Cavalieri’s principle. The brains wereserially cut using a salami slicer machine (Elta GmBH, Roedermark, Germany)with a mean thickness of 8.25 mm from rostral to caudal end and averages of 11sections were obtained from the brains. The brain volume was 348.00 ± 50.48 cm3,175.70 ± 11.11 cm³ in gray matter volume and 172.30 ± 42.90 cm³ in white mattervolume. Volume ratios of gray and white matter were 50.98% and 49.02%respectively. The ratio of brain weight to brain volume was 1. The ratio ofgray and white matter in the brain of the ox is similar to that of the gray andwhite matter in the human brain, but that the sheep and horse are different. 
      PubDate: Thu, 01 Mar 2018 00:00:00 +030
       
  • Developments in Live Cattle and Beef Import and the Analysis of Producer
           Prices with Trend Models in Turkey

    • Authors: Hasan ÇİÇEK; İlkay DOĞAN
      Abstract: In this study, developments in live cattle and beef import evaluated and current and fixed beef prices and fattening feedprices were analyzed by trend models in Turkey. Study materials are live cattle and beef imports (quantity and value)between 2010-2016 years, current fattening feed and beef producer prices between 1998-2017 years and monthly producerprices index. Current prices have been converted to fixed prices using the monthly producer prices index. Within the scopeof trend analysis, price estimates were made for 2018-2020 period. Although the current fattening feed prices and beefproducer prices have increased by about 17 times in the period examined, it has been determined that both are falling inreal terms. It has been determined that live cattle and beef imports partially affect domestic market prices, changes inexchange rates over the years reflected the cost of imports, and the increase in feed prices puts more pressure on beefproducer prices. A quadratic model has been chosen for beef and feed prices according to trend analysis accuracy criteria.According to this; when the average price of beef from January to September 2017 (26,13 TL/Kg) is considered; it isestimated to increase by about 18% (30,78 TL/Kg) in real terms until the end of 2020 (Y=29,93-1,003t+0,0452t2). Whenthe average price of fattening feed is taken into consideration from January to September 2017 (0,90 TL/Kg); it isestimated to increase by about 31% (1,18 TL/Kg) in real terms until the end of 2020 (Y=1,056-0,047t+0,0023t2). 
      PubDate: Thu, 01 Mar 2018 00:00:00 +030
       
  • Determination of External Ear Canal Problems Using Radiographic,
           Ultrasonographic and Video-Otoscophic Methods

    • Authors: Nusret APAYDIN; Özge HASANDAYIOĞLU
      Abstract: Thepurpose of this study is the determination of external ear canal diseases indogs by applying radiological, ultrasonographic, and video-otoscopic methods,comparison of these methods, and their advantages and disadvantages. Thematerial of the study consisted of 31 dogs of different breed, age, and sexwith ear diseases. Patients were examined by sedation or general anesthesiawhen necessary. Eachcase was examined with video-otoscopy, ultrasonography and radiology. All theimages and data from these examinations were recorded.Twenty-nine cases with video-otoscopic examination, 3cases with radiological imaging, and 25 cases with ultrasound findings werediagnosed with a definite diagnosis. As a result, it has been determined that thevideo-otoscopic method is more advantageous than the other methods in thedefinite diagnosis of external ear canal diseases in dogs. The ultrasonographicmethod can not be sufficient by itself and the radiographic method can beapplied in specific cases.
      PubDate: Thu, 01 Mar 2018 00:00:00 +030
       
  • First Report of Escherichia coli O157 From Traditional Turkish Dairy
           Product Kaymak

    • Authors: Didem SAĞLAM; Esra ŞEKER
      Abstract: The aim of this study was to isolate the E. coli O157:H7/H- serotype from traditional Turkish dairy product kaymak sold in Afyonkarahisar, investigate the Stx1, Stx2, ehlyA and eaeA genes in isolated strains by PCR and determine the antibiotic resistance of strains to some antibiotics commonly used in Turkey. For this purpose, a total of 100 kaymak samples sold in public bazaars located center town and villages of Afyonkarahisar were collected. E. coli O157 was isolated from 3 of 100 samples by using conventional culture methods and serological confirmation tests. The presence of Stx1, Stx2, ehlyA and eaeA genes in the isolated 3 strains was investigated by PCR. While all of 3 strains harboured the Stx1 and ehlyA genes, the Stx2 and eaeA genes were not found in the strains. The antibiotic resistance of strains was investigated by Kirby-Bauer disc diffusion test. While all of strains were resistant to cephazoline, cefoxitin and ceftiofur, the resistance was also determined in the strains to ampicillin (66.7%), cephalothin (66.7%), ceftriaxone (33.3%), nalidixic acid (33.3%) and trimethoprim/sulphamethoxazole (33.3%). The present study is the first investigation showing the presence of E. coli O157 and its virulence genes in the kaymak in Turkey.
      PubDate: Thu, 01 Mar 2018 00:00:00 +030
       
  • Apoptotic and Non-Apoptotic Effects of Granzyme

    • Authors: Ercan KESKİN; Durmuş HATİPOĞLU
      Abstract: Granzymes (granule enzymes) are proteases that are released from the granules of cytotoxic lymphocytes into target cells to protect mammals from altered cells and virus infections. Granzymes released into the target cell cytoplasm activate some specific pathways to effect the death of these cells. Although the realization of death of the target cell is regarded as the main function of these proteases, the findings show that they have other functions besides cell death. Granzymes can display antiviral activity directly by breaking down the proteins they encode so that the virus can survive life cycles in the host. The increased levels of circulating granzymes and disintegration of extracellular substrates into granules in various inflammatory processes indicate that these proteases may have extracellular effects on pathogenesis of chronic inflammatory diseases, rejection of tumor cells and viral infections.
      PubDate: Thu, 01 Mar 2018 00:00:00 +030
       
  • Isothermal Amplification Methods Most Used in the Diagnosis of Infections

    • Authors: Mehmet ÜVEY; Nilgün ÜNAL
      Abstract: Isothermal amplification of nucleic acid is one of the most commonly used methods to identify the infections, tumors, genetic abnormalities, and in forensic medicine. As isothermal amplification methods can be performed at one reaction temperature under simple conditions they are less complex in comparison to Polymerase Chain Reaction (PCR). These methods differ from each other with the diversity of enzymes, primers used, sensitivity and specificity. In this review, the most commonly used isothermal amplification methods, their principles, areas of use and innovations brought about are discussed.
      PubDate: Thu, 01 Mar 2018 00:00:00 +030
       
  • No title

    • Authors: Kozet AVANUS; Lora KOENHEMSI
      PubDate: Thu, 01 Mar 2018 00:00:00 +030
       
  • Estimated Genetic Diversity Between Atak-S and Isa Brown Chickens with SSR
           Markers

    • Authors: Selçuk ÖZDEMİR; Harun ARSLAN, Uğur ÖZENTÜRK, Fatih YILDIRIM, Ahmet YILDIZ
      Abstract: Atak-S layered hybrid, developed by the "Ankara Poultry Research Institute", is the hybrid with the highest egg productivity among local hybrids. However, egg yield are lower compared to foreign-origin hybrids such as Isa brown. Molecular development studies as to productivity are required for Atak-S hybrid to be able to compete with foreign-origin hybrids. The objective of this study was to detect genetic diversity within the breeds and to compare the Atak-S and Isa Brown breeds. To achieve this goal, we individually genotyped 6 SSR loci in 200 chickens from two populations. 85 polymorphic fragments were found in total for 6 different SSR markers employed in this study. For all loci, high He was observed, and means He was 0.61±0.05 among loci. Among breeds, the mean He was 0.35±0.76 in Atak-S and 0.26±0.68 in Isa Brown. This result indicated that genetic diversity in the Atak-S breed is higher than in the Isa Brown breed. Furthermore, genetic differences and population structure of two hybrids were shown by virtue of dendogram analysis and differences were detected. As a result, we could be suggested that genetic diversity is well preserved within and between Atak-S and Isa Brown hybrids. Based on the Atak-S and Isa Brown breeds estimated by microsatellite analysis may be useful as a guide in setting goals for designing future genetic variation studies and for improving conservation strategies.
      PubDate: Thu, 01 Mar 2018 00:00:00 +030
       
  • Assessment of Echocardiographic Ejection Fraction and Fractional Shortness
           at Different Stages of Canine Visceral Leishmaniasis

    • Authors: Canberk BALIKCI; Kerem URAL
      Abstract: In this study, it was aimed to evaluate EF and FS parameters at different stages of canine visceral leishmaniasis to benefit in diagnosis and treatment monitoring. A total of 35 dogs, 28 of which were infected with L. infantum and 7 of which were healthy, constituted the material of study. VL diagnosis was confirmed with test kit positivity with fast ELISA on blood samples. VL dogs eligible for inclusion into study were evaluated in 4 different groups (n = 7, each group), according to "LeishVet Working Group". ECHO, studies were performed on the right parasternal short axisand EF and FS parameters were detected. In control group, EF values (%) was found between 48 and 74, FS(%) value between 24 and 38. EF value was found between 59 and 74,FS valuewas between 31 and 42 in group I. EF value was found between 58 and 93, FS value was between 29 and 63 in group II. EF value was foundbetween 35.3 and 93.8, FS value wasbetween 16.4 and 50 in group III. EF value was between 52 and 82, FS value was between 27 and 50 in group IV. As a result, when we considered the LWG, it can be said that echocardiographic evaluation [reduction in FS, EF (systolic dysfunction)] should be performed into account in CVL dogs. It may be beneficial to focus on cardiac changes contributing to the intravital diagnosis in CVL dogs and to apply the necessary additional treatment protocols.
      PubDate: Thu, 01 Mar 2018 00:00:00 +030
       
  • The Effects of Liquid Dishwashing Detergent Exposure in Male Swiss Albino
           Mice

    • Authors: Özlem YILDIZ GÜLAY; Ayhan ATA, Ahu DEMİRTAŞ, Şükrü GÜNGÖR, Mehmet Şükrü GÜLAY
      Abstract: The current study investigated the possible effects of liquid dishwashing detergent on some blood parameters and organ weights of male Swiss Albino mice. A total of 40 male Swiss albino mice were used in the experiment. Mice were randomly assigned to 5 treatment groups (n=8 per group). Mice in the control group (TR1) were supplied with tap water. Meanwhile, mice in TR2, TR3, TR4 and TR5 groups were given 0.1, 0.5, 1 and 5% v/v of the liquid detergent in tap water, respectively. The treatments started at 3 wk of age and continued for 60 d. Mice were kept under standard laboratory conditions in separate plastic cages. Food and water were provided ad libitum. At the end of the 60 d treatment period, blood samples were collected from the beating hearth under anesthesia. After sacrificed, the organs of the mice were weighted. There were no significant alterations on hematocrit, RBC, WBC, MCV, MCH, MCHC, total lymphocyte, monocyte or basophil levels (P>0.1) due to detergent exposure. Similarly, treatments had no effect on liver, kidney, spleen, lung, testis or brain weights (P>0.1). However, ingestion of detergent adversely affected hemoglobin (P < 0.01), plasma protein (P<0.05), total neutrophil (P<0.03) and eosinophil (P<0.03) levels. Furthermore, ALP and AST levels were significantly increased in TR4 and TR5 (P<0.05) due to detergent exposure. Thus, the results of the current study imply that particularly the higher doses of liquid dishwashing detergent could be toxic and cause health risks to male Swiss albino mice.
      PubDate: Thu, 01 Mar 2018 00:00:00 +030
       
 
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