Journal Cover Jurnal Sain Veteriner
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  This is an Open Access Journal Open Access journal
   ISSN (Print) 0126-0421 - ISSN (Online) 2407-3733
   Published by Universitas Gadjah Mada Homepage  [28 journals]
  • Mozart KV 448 Menurunkan Densitas dan Aktivitas Neuroglia Hipokampus
           Mencit (Mus musculus) Selama Stres Prenatal No. 416-KE

    • Authors: Shelly Kusumarini, Lita Rakhma Yustinasari, Eka Pramystha Hestianah, Suryo Kuncorojati, Tutik Juniastuti
      Pages: 1 - 8
      Abstract: The aim of this research was to explore the influence of Mozart KV 448 classical music therapy to the neuroglia cells of mice’s hippocampus that were exposed to stress during prenatal. This research were employing twenty female mices and twenty male mices. Female mices were estrus synchronized with PMSG and hCG then monomating to the males. Pregnant females were then divided into four groups (P0, P1, P2, P3). P0 was as control, P1 was treated by one minute forced swim test, P2 was treated by one minute forced swim test followed by thirty
      minutes classical music Mozart KV 448 and P3 was treated by one minute forced swim test followed by sixty minutes classical music Mozart KV 448. This research was carried out for twenty-one days during gestation period. The neuroglia density result was analyzed using ANOVA and Duncan test. The neuroglia activity result was
      analyzed using Kruskal wallis test and Z test. The histology reading showed degradation of density and activity of hippocampus neuroglia.
      PubDate: 2017-10-24
      DOI: 10.22146/jsv.29279
      Issue No: Vol. 35, No. 1 (2017)
       
  • Penyakit Ginjal Kronis pada Anjing dan Kucing: Manajemen Terapi dan Diet

    • Authors: Yanuartono Yanuartono, Alfarisa Nururrozi, Soedarmanto Indarjulianto
      Pages: 16 - 34
      Abstract: Chronic kidney disease is a progressive impairment of renal function and irreversible. The kidneys fail to maintain metabolism and fluid and electrolyte balance, causing uremia. This disease is a common problem in old cats and dogs that not detected by the owners up to 75 % of kidney function is damaged. Clinical signs vary as polyuria, polidipsi, anorexia, vomiting, weight loss, pale mucous membranes, mouth ulceration, halitosis and acute blindness. Chronic renal failure is not curable so that the necessary medication management and proper diet in orderto improve the quality of life and prolong the life of the animal.
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29284
      Issue No: Vol. 35, No. 1 (2017)
       
  • Gambaran Histopatologi Rumen dan Retikulum Sapi Bali Akibat Adanya Benda
           Asing

    • Authors: Eldarya Envisari Depari, Annas Farhani, I Wayan Batan, I Made Kardena
      Pages: 35 - 41
      Abstract: Rumen and reticulum hold an important role in the ruminant’s digestive tract. During the meal, Bali cattle accidentally ingest foreign objects because they can’t distinguish between foreign body object and feed. Cattles do not really chew food before swallowing. This study aims to determine the existence of a foreign body in the stomach and histopathological overview of Bali cattle rumen and reticulum due to a variety of foreign objects. This study used 10 samples of cattle Bali consisting of nine samples of rumen and reticulum contained foreign objects (plastic, metal, wood and stone) and one normal sample of the rumen and reticulum Bali cattle. Samples were obtained from Slaughter House at Mambal, Abian Semal District, Badung regency, Bali. Based on the results of research conducted on the rumen and reticulum Bali cattle, there are a variety of foreign objects such as plastic, metal, wood and stone. Histopathological changes in the mucosa of the rumen and reticulum form of inflammatory cell infiltration from mild to severe. Conclusions from this research that found a foreign body object in the rumen and reticulum Bali cattle, and foreign body objects cause tissue destruction.
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29288
      Issue No: Vol. 35, No. 1 (2017)
       
  • Kemampuan Reagen Curcumax Mendeteksi Boraks dalam Bakso yang Direbus

    • Authors: Erna Dwi Astuti, Widagdo Sri Nugroho
      Pages: 42 - 48
      Abstract: Borax is a harmful compound to human health but sometimes was used illegally as preservative in meatball. Curcumax reagent is a compound which developed from the previous borax detector base on tumeric extract. This reagen was developed as an easy kit to apply in the field. This study aims were to determine accuracy of sight obesrvation and spectrophotometer to detected borax in boiled meatballs which tested by Curcumax and to determine the effect of boiling on the borax presence in the meatballs. This study was using meatballs group
      as a control and another meatballs group preserve with borax 0.5%. Each treatment group was boiled in 0, 5, 10, and 15 minutes and each treatment unit had three replications which consist five meatballs each of them. Borax
      detection was done by mixing 1ml Curcumax reagent into 1 ml meatball extract. Qualitative examination of borax presence in the meatballs was known based on the color changing of tested meatball’s extract from yellow became orange (direct sight with eyes). The Quantitaive analysis was done by measured using spectrophotometer base on wave-length (λ) at 570 nm. Data was analyzed with ANOVA and continued by Least Significant Difference (LSD) analysis. Borax detection using curcumax based on sight sense against color change giving an inconsistence result. Meanwhile quantitative measurement by spectrophotometer more accurate than one. Curcumax had been detected borax in meatball which boiled for 15 minutes.
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29289
      Issue No: Vol. 35, No. 1 (2017)
       
  • Korelasi Berat Badan dan Umur Sapi terhadap Berat, Volume Cairan dan
           Konsentrasi Prostaglandin F2α pada Vesikula Seminalis

    • Authors: Naela Wanda Yusria Dalimunthe, Agung Budiyanto, Erna Prawita Setyowati, Agustina Dwi Wijayanti
      Pages: 49 - 56
      Abstract: Seminal vesicles were collected from 60 heads of Bulls which butchered in slaughter house (RPH) Yogyakarta. The aims of this study are knowing the relationship between body weight, age, fluids volume and concentration of prostaglandin F2 α (PGF2α) in seminal vesicle of beef cattle. Those seminal vesicles were gathered from bulls which recorded its body weight and age then measured its seminal vesicles for weight, fluids volume and PGF2α levels. The PGF2α level was measured by Enzyme-Linked Immunosorbent Assay. Statistical analysis was performed using one way – analysis of varian, regression and correlation with P<0.05. Body weight of bulls showed positive correlation with the weight of seminal vesicle and its fluids volume. However, PGF2α levels were not correlated with the body weight of cattle. Weight of seminal vesicles also exhibited positive correlation with volume of vesicle fluids but no correlation with PGF2α levels. Based on the age of bulls, there were no correlation with
      the weight of seminal vesicles, seminal fluids volume and PGF2α levels. Those result indicated that the weight and fluids volume were affected by the body weight of bulls altough the PGF2α levels have a standard of development
      which seems affected by other factors such as concentration of androgen hormone.
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29291
      Issue No: Vol. 35, No. 1 (2017)
       
  • Kerusakan Usus pada Mencit (Mus musculus) yang Diinokulasi Larva 3 (L3)
           Anisakis spp.

    • Authors: Febrina Dian Permatasari, Poedji Hastutiek, Lucia Tri Suwanti
      Pages: 57 - 62
      Abstract: This study aims to be show damage in the intestine of mice caused by inoculation of the larvae of Anisakis spp. alive and who has died because of a warming 750C for 7 minutes. Eighteen male mice (Mus musculus) were divided into three groups: control group, a group of mice were inoculated the larvae of Anisakis spp. alive and
      groups of mice were inoculated the larvae of Anisakis spp. who have died. 48 hours post inoculation, the mice’s intestines necropsy performed later performed HE staining to identify and scoring intestinal histopathology. The results showed inoculation of the larvae of Anisakis spp. either alive or dead induce histological changes in the intestine in the form of infiltration of inflammatory cells, epithelial changes and structural changes in the intestinal mucosa
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29292
      Issue No: Vol. 35, No. 1 (2017)
       
  • Perlakuan Celup Puting setelah Pemerahan terhadap Keberadaan Bakteri
           Patogen, Staphylococcus aureus, Streptococcus agalactiae, dan E. coli pada
           Sapi Perah Penderita Mastitis Subklinis di Peternakan KUNAK Bogor

    • Authors: Herwin Pisestyani, Etih Sudarnika, Rachmi Ramadhanita, Abdul Zahid Ilyas, Chaerul Basri, Ardilasunu Wicaksono, Arifin Budiman Nugraha, Mirnawati B Sudarwanto1
      Pages: 63 - 70
      Abstract: Mastitis is udder inflammation that can be caused by the entry of pathogenic bacteria through the teat. One of the treatments that can prevent bacteria infection is teat dipping. This research was aimed to identify the effect
      of teat dipping after milking to the presence of pathogenic bacteria that cause subclinical mastitis (Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli) and the presence of pathogenic bacteria that cause subclinical
      mastitis by quarter of udder. Milk samples were taken from normal lactation period of dairy cows, clinically healthy, and positive subclinical mastitis status when the intervention was applied. Sixty seven samples of quarters from 18
      dairy cows were identified to the presence of pathogenic bacteria that cause subclinical mastitis before teat dipping and at 1st, 2nd, and 3rd months after teat dipping. Identification of the presence of pathogenic bacteria was done by
      culturing samples on selective agar media. The presence of common pathogenic bacteria, Staphylococcus aureus, and Escherichia coli decreased significantly after teat dipping, while Streptococcus agalactiae and other pathogenic
      bacteria did not decrease significantly. The presence of pathogenic bacteria based on the quarter of udder did not show the significance difference. Application of teat dipping after milking was able to decrease the presence of
      pathogenic bacteria that cause subclinical mastitis.
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29293
      Issue No: Vol. 35, No. 1 (2017)
       
  • Pengendalian Folikulogenesis Ovarium dengan Pemberian Ekstrak Biji Kapas

    • Authors: Siska Adelya Ramadhani, Iman Supriatna, Ni Wayan Kurniani Karja, Adi Winarto
      Pages: 71 - 80
      Abstract: Gosipol is a substances contained in extracted cotton seed which is thought to have the antifertility ability therefore it is often used as a herbal contraceptive. The aim of this study were to assess the folliculogenesis in mice after administrated with cottonseed extract. 60 female mice strain DDY which was 14-15 weeks old and 30-35 g body weight were divided into five groups and given cottonseed extract each 0; 1,5; 2,1 and 2,7 g/kg BW for 5, 10, 15, 24, and 24 + 10 days (without cottonseed treatment). At the end of the treatment period, mice was euthanasia to observe follicular development histomorphology (each three mice of each treatment). Mice estrous status were evaluated based on the description of the vaginal smear cells with Giemsa staining. The results showed
      that the number of developing follicles was low (P < 0.05) compared with control after 5 days cottonseed extract administration at dose 2,7 g/kg BW that were 23 ± 3,6. At dose 1,5 and 2,1 g/kg BW the number of follicles was low after 24 days that were 25 ± 10,4 and 27 ± 3,5. Recovery effects of follicle number after cottonseed extract administration for 24 days was the best at a dose of 1,5 g/kg BW. Prolonge of estrous cycle occured in mice which were administrated the cottonseed extract of at all dose treatment. In conclusion, although the decrease in the number of developing follicles and prolonge of estrous cycles occurred after cottonseed extract administration, but these effects are reversible after the administration ended.
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29294
      Issue No: Vol. 35, No. 1 (2017)
       
  • Respons Imun Ayam Petelur Pascavaksinasi Newcastle Disease dan Egg Drop
           Syndrome

    • Authors: Gusti Ayu Kencana, I Nyoman Suartha, Daniel Raja Bonar Nainggolan, Agatha Seren Lumban Tobing
      Pages: 81 - 90
      Abstract: Some viral diseases in poultry could lead to huge losses to the farmers. Newcastle Disease (ND) and Egg Drop Syndrome (EDS) are a group of infectious viral disease that can cause the decreasein egg production. Newcastle Disease is caused by Avian paramyxovirus type 1 (PMV-1) Paramyxoviridae family. The causative agent of EDS is Duck adenovirus-I Adenoviridae family. Both of these diseases affect the economic losses to the poultry. The main action to prevent hens from ND and EDS virus diseases is vaccination. The success of
      vaccination can be tested by serology. ND and EDS virus characteristically agglutinate hen’s erythrocyte they have Hemagglutine protein on virus envelope so can be tested by hemagglutination. The study was conducted on
      a commercial poultry farm in order to determine the success of vaccination against ND and EDS. The hens were vaccinated by Newcastle Disease-Infectious Bronchitis- Egg Drop Syndrome (ND-IB-EDS) inactivated vaccines.
      Serological test was conducted in pre and post vaccination by using microtiter hemagglutination test. The antibody titre is expressed in units of HI log2. The results of the study, the mean antibody titer against ND pre vaccination
      was 4,53 ± 1,356 HI log2 and antibody titre in 2nd, 3rd and 4th week were 8,67 ± 0,617 HI log2, 7,73 ± 1,335 HI log2 and 5,20 ± 0,862 HI log2 post vaccination. Antibody titre against EDS pre vaccination was 0 ± 0,000 HI log2 and antibody titre post vaccination in 2nd, 3rd and 4th week were 7 ± 1,363 HI log2, 7,27 ± 1,438 HI log2 and 7,6 ± 1,056 HI log2. It showed that ND-IB-EDS inactivated vaccines is serological protective for ND and EDS titres.
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29295
      Issue No: Vol. 35, No. 1 (2017)
       
  • Ekspresi IL – 1β pada Proses Kesembuhan Fraktur Os Femur Tikus Putih
           (Rattus norvegicus) Ovariektomi yang Diterapi dengan Ekstrak Cikal Tulang
           (Cissus quadrangularis)

    • Authors: Ira Sari Yudaniayanti, Bambang Sektiari Lukiswanto, M. Zainal Arifin
      Pages: 91 - 101
      Abstract: This study aims to determine the potential of the Cissus quadrangularis extract to decreased the expression of IL-1 β so that it can to inhibit bone resorption and osteoporosis fracture healing is not delayed union. Forty rats adapted for 1 week, and then divided into four groups with 10 replications. P0 (sham-operated + Osteotomy + CMC Na); P1 (ovariectomy + Osteotomy + CMC Na); P2 (ovariectomy + Osteotomy + Raloxifene 5.4 mg /kg); P3 (ovariectomy + Osteotomy + CQ 750 mg / kg). Osteoporosis induced is made by bilateral ovariectomy in rats, then to make sure that osteoporosis is already happened, 8 weeks post-ovariectomy performed radiology examinations on the femur, then the osteotomy action. IL1-β expression observations performed in at the 2nd week and 6th week after osteotomy through the immunohistochemistry examination. The results of immunohistochemistry examination in the 2nd week showed an increased expression of IL1-β in all groups of rat that did ovariectomy (P1, P2 and P3) were significantly different (p <0.05) with a rat group that didn’t ovariectomy, as well P2 dan P3 were significantly different with P1. The Results of immunohistochemistry examination in the 6th week showed the same
      pattern with the 2nd week and there is increased IL1-β expression in all groups of rat compared with the results of the examination in the 2nd week. Based on these results it can be concluded that Cissus quadrangularis extract and
      Raloxifen can decreased the expression of IL-1 β, and the results that don’t significantly different, so that these both materials can to inhibit bone resorption and osteoporosis fracture healing don’t get delayed union.
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29296
      Issue No: Vol. 35, No. 1 (2017)
       
  • Ekstrak Air Rumput Kebar (Biophytum petersianum Klotzch) sebagai
           Penghambat Perkembangan Telur Cacing Gastrointestinal Ruminansia Secara in
           Vitro

    • Authors: Alnita Baaka, Isti Widayati, Noviyanti Noviyanti
      Pages: 102 - 110
      Abstract: Gastrointestinal worm disease in cattle is scathing for the breeder’s as it lowers cattle’s productivity and inflict major economic loss. Furthermore, the interraction between cattle, man and the environment might bring
      out the risk of zoonotic diseases (diseases which can spread between man and animals). Kebar grass contains phytochemical elements such as alkaloid, saponin, tanin, glycoside and flavonoid. Those elements have the potential
      as an alternate anthelmentic in the effort to utilize the endemic biodiversities of Papua. This research is performed at Faculty of Animal Husbandry Animal Health Laboratory and Faculty of Math and Natural Science Microbiology
      Laboratory of Universitas Papua, for 8 months. The leaves and the stems of the kebar grass were collected and stored at room temperature. The Kebar grass extract was divided into 3 parts, i.e. fresh extract, wind dried extract
      and sun dried extract. Fresh fecal samples were collected and examined with the native method. The samples where nematodes eggs are found is treated with kebar grass extract. As a comparison, positive control is made with
      oxfendazole and negative control is made nematodes larvae fertilization. Fecal cultures and grass extract mixture is left alone for 7 days in room temperature and examined under microscope with 400x fortification to see whetner
      there is any development of the nematodes’ eggs. The obstained data is analyzed by tabulation and descriptive method. The result is the samples mixed with kebar grass extract show no development of nematodes’ egg. As a
      comparison, the negative control which is observed with baerman method shows development of nematodes’ eggs. It can be concluded that the effect of kebar grass extract can deters nematodes’ egg development.
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29297
      Issue No: Vol. 35, No. 1 (2017)
       
  • Morfologi Hemipenis Biawak Air (Varanus salvator bivittatus)

    • Authors: Mahfud Mahfud, Adi Winarto, Chairun Nisa
      Pages: 111 - 117
      Abstract: Varanus salvator bivittatus has a pair of hemipenes, which is macroscopic anatomically like the copulatory organ in other amniotes, cylindrical-shaped (truncus) with quite flexible, and it located on the base of the caudal tail
      of the cloaca. However, information about the microscopic anatomy of hemipenes of this animal is scientifically unpublicized. Therefore, the aim of this research is to study the microscopic anatomy of hemipenes of male Varanus
      salvator bivittatus. The animals were sacrificed by exsanguination under deep anesthetized and fixed in 4 % paraformaldehyde through perfusion then observed visceral site and morphometric. Histomorphological evaluation
      was obtained by paraffin preparation with section thickness of 3-4 μm then stained in Hematoxylin-Eosin (HE) and Masson’s Trichrome (MT). The results showed that truncus of hemipenes was lined by stratified squamous
      epithelium and supported with thick of dense connective tissue and contain cavernous body and blood vessels are found, the muscles not found. The presence of connective tissue that supported in the down part sometimes
      make hemipenes are rigid while prurient condition. In the caudal of truncus hemipenes there is retractor muscle of hemipenes which arranged by striated muscles. Hemipenes is flexible because contain with much of blood vessel
      that found in truncus hemipenes.
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29298
      Issue No: Vol. 35, No. 1 (2017)
       
  • Pendekatan Diagnosis Avian Influenza Virus dan Newcastle Disease Virus
           pada Kasus Lapangan Ayam Petelur: Imunopatologis Streptavidin Biotin

    • Authors: Maya Ekaningtias, Hastari Wuryastuty, Wasito Wasito
      Pages: 118 - 126
      Abstract: Poultry can be infected not only by one type of virus, but also by more than one type of virus. Avian influenza virus (AIV) and Newcastle disease virus (NDV) are two important pathogens in poultry that can cause recurrent outbreaks and often lead to clinical symptoms and pathological lesions similar in poultry with high morbidity and mortality causing significant economic losses to the poultry industry. This study aims to detect and differentiate pathogenic viruses in commercial laying chickens with similar clinical symptoms, especially torticolis and curled toe paralysis, and anatomic pathological lesions, such as foci necrotic hemorrhages in the digestive tract by applying streptavidin-biotin immunohistochemical immunological test (IHC SB). Samples (chickens) were taken from the cases of the disease in poultry in several commercial poultry farms. The layer chickens in which showed clinical symptoms of torticolis and curled toe paralysis, and hemorrhagic lesions in the lungs and / or digestive system are suspected of having AIV and NDV infections. After being necropsied, then lungs and gastrointestinal tract were tested IHC SB. The IHC SB results were examined with a digital microscope camera system. The results of the present study indicated that AIV antigen was detected in the lungs, but not in the digestive tract, whereas, NDV was detected in the digestive tract, but not in the lungs. It was concluded, that the IHC SB can be applied for confirmation of the diagnosis of AIV and NDV and indicated, that the commercial laying chickens with clinical symptoms of torticolis and curled toe paralysis, as well as pathological hemorrhagic lesions in the gastrointestinal tract could be infected by both viruses.
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29299
      Issue No: Vol. 35, No. 1 (2017)
       
  • Newcastle Disease Virus Detection from Chicken Organ Samples Using Reverse
           Transcriptase Polymerase Chain Reaction

    • Authors: Lehgarubini Shanmuganathan, Dito Anggoro, Michael Haryadi Wibowo
      Pages: 127 - 135
      Abstract: Newcastle disease (ND) is a systemic, viral respiratory disease that is acute and easily transmitted which affects various types of poultry, especially chickens. Diagnosis of ND which generally involves virus isolation and subsequent identification with serological assays has limitations that needs more time. This research was aimed to detect Newcastle Disease virus (NDV) in chickens suspected with ND using the Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) technique. Nine chicken organ samples such as lien, trachea, and lungs were collected from chicken farms diagnosed with ND. The organ samples were processed and the targeted viral RNA was extracted using the RNA extraction kit. Genome amplification was performed with RT-PCR using specific
      primers to target the F gene. Amplification results produced an amplicon product of 565 base pairs (bp). PCR product samples were then visualised using agar gel electrophoresis and viewed using the unified gel documentation system. Amplification results show nine samples positive for the DNA bands corresponding to the targeted band of the NDV F gene fragment. The results of this research confirm that the RT-PCR method is applicable for NDV detection from chicken organ samples.
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29300
      Issue No: Vol. 35, No. 1 (2017)
       
  • Optimalisasi Waktu Pengomposan dan Kualitas Pupuk Kandang dari Kotoran
           Kambing dan Debu Sabut Kelapa dengan Bioaktivator PROMI dan Orgadec

    • Authors: Linda Trivana, Adhitya Yudha Pradhana
      Pages: 136 - 144
      Abstract: Animal manures can be used as organic fertilizer because the high nutrient content such as nitrogen (N), phosphorus (P), and potassium (K). The nutrients are needed by plants and soil for fertility. One of the animal manures
      that can be used for organic fertilizer is goat manure. Goat manure is used as organic fertilizer it contains relatively more balanced nutrients than other natural fertilizer. In addition, the goat manure is mixed with the goat urine that also
      contains high nutrients. Coconut coir dust contains high potassium so that the addition of coconut coir dust in organic fertilizer will increase the potassium content (K) in organic fertilizer. The purpose of this research was to find out the
      influence of effective Orgadec and PROMI bioactivators on the optimal time of composting and the quality of the organic fertilizer. The organic fertilizer was analyzed to determine the content of N, P, K, organic-C, C/N ratio, water
      content, and pH. The result of analysis is compared with SNI 19-7030-2004. Organic fertilizer analysis was conducted on day 0, 10, 20, 30, 40, and 50. The result of the organic fertilizer analysis with Orgadec bio-activator more effective
      and faster on the decomposition organic matter in the compost compared PROMI (<20 days). The quality of organic fertilizer with Orgadec bioactivator on day 10 and 20, while organic fertilizer with PROMI on day 20, 30, 40, and 50
      days was in accordance with the quality stated in SNI 19-7030-2004 (C/N ratio, levels of N, P, K, water, and organic-C).
      PubDate: 2017-06-01
      DOI: 10.22146/jsv.29301
      Issue No: Vol. 35, No. 1 (2017)
       
 
 
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