Journal Cover Journal of the Medical Sciences (Berkala ilmu Kedokteran)
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  This is an Open Access Journal Open Access journal
   ISSN (Print) 0126-1312 - ISSN (Online) 2356-3931
   Published by Universitas Gadjah Mada Homepage  [27 journals]
  • Comparison of Bcl-xL protein expression in placental trophoblast cells
           between pregnancy complicated by severe preeclampsia and normotensive

    • Authors: Diah Rumekti Hadiati, Arsi Palupi, Mohammad Hakimi, Sofia Mubarika Haryana
      Abstract: Background: Preeclampsia is one of the main causes of maternal and perinatal mortality and morbidity. The pathogenesis of preeclampsia remains unclear but it is believed that the failure of spiral arteries remodeling will eventually leads to placental hypoxia. In pregnancy complicated by preeclampsia, trophoblast apoptosis is considered to be excessive. Although this hypothesis is still under study, it is believed that regulation of apoptosis in trophoblast cells plays a key role in the pathophysiology of preeclampsia. The mechanism of molecular apoptosis in human is very complicated and involves many signaling molecules, one of them is Bcl-2 protein. Bcl-2 protein group consists of proapoptosis (Bax) protein and apoptosis inhibitor (Bcl-2 and Bcl-xL) protein.Objective: To compare the expression of Bcl-xL protein in placental trophoblast cells between pregnancy complicated by severe preeclampsia and normotensive pregnancy.Methods: The design of this study was cross sectional design. The population were patients with severe preeclampsia and normotensive patients which were treated in Dr. Sardjito Hospital, Yogyakarta, Indonesia from October 2011 until March 2012. Placenta samples were obtained from 43 patients with pregnancy complicated by severe preeclampsia and 38 patients with normotensive pregnancy. Bcl-xL protein expression was observed using immunohistochemistry technique. Statistical analysis was conducted using independent t-test (p<0.05), bivariate analysis using chi-square test, and multivariate analysis using logistic regression.Results: There was significant difference in Bcl-xL protein expression in trophoblast cells between pregnancy complicated by severe preeclamptic group (1,29 ± 0,12) compared to normotensive group (1,71 ± 0,14) with p=0.00. Multivariate analysis using logistic regression showed that diagnosis of severe preeclampsia had a statistically significant role in Bcl-xL protein expression with p= 0.000.Conclusion: Expression of Bcl-xL protein is lower in pregnancy complicated by severe preeclampsia compared to normotensive pregnancy.
      PubDate: 2017-06-21
  • Molecular Mechanism of Synthesized Potential Anticancer Agent Chalcone in
           Leukemia Cell Line K562

    • Authors: Arina Novilla, . Mustofa, Indwiani Astuti, . Jumina, Hery Suwito
      Abstract: Chalcone, a precursor of flavonoids that are abundantly found in plants, has been investigated and found to possess anticancer activity. This study aimed to evaluate the effect of chalcone derivatives on the PI3K/AKT signaling pathway in the K562 cell line. A K562 cell line was treated with two chalcone derivatives: (E)-1-(4-aminophenyl)-3-(2,3dimethoxyphenyl)-prop-2-en-1-one (Compound 4), and (E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one) (Compound 7), and compared to the anticancer drugs imatinib and accutane. Measurement of AKT caspase-3, STAT3, and cyclin D1 were performed with ELISA, whilst cell cycle and apoptosis analysis were performed using flow cytometry. Both Compound 4 and Compound 7 at all concentrations showed the most significantly decreased AKT levels in K562. Compound 4 at 5 and 10 µg/mL, and Compound 7 at 10 µg/mL, significantly increased caspase-3 on K562, although this was lower than when using imatinib. Compound 4 and Compound 7 in all concentrations showed decreasing STAT3 on K562, which were comparable to imatinib. Compound 4 and Compound 7 showed the highest decrease of cyclin D1 among treatments, and also arrested  G0/G1 and G2/M phase in K562 cells, with the results being comparable to imatinib and Isotretinoin. Compound 4 and Compound 7 are promising anticancer agents that function via inhibition of the PI3K/AKT signaling pathway, induction of apoptosis through up-regulation of apoptotic markers, and blockade of cell cycle progression by regulating cell cycle-related factors.
      PubDate: 2017-06-21
  • Comparing P-Selectin (CD62P) expression in patients receiving
           non-leukodepleted vs leukodepleted thrombocyte concentrates

    • Authors: Teguh Triyono
      Abstract: Thrombocyte concentrate (TC) transfusion is an important supportive therapy in patients with thrombocytopenia. The risks in platelet transfusions may be related to the content of TC including the contaminant leukocytes. The aim of this study was to assess the risk of increased level of P-Selectin (CD62P) expression of non-leukodepleted TC transfusions.This was a quasi-experimental study. Subjects were children patients aged 1-18 years who received a non-leukodepleted or a leukodepleted TC transfusions. Comparison of the proportion of  increased expression of CD62P in both groups expressed as relative risk. The subjects consisted of 51 patients who received non-leukodepleted and 52 patients who received leukodepleted TC transfusions. The risk of increased expression of CD62P in patients receiving non-leukodepleted TC transfusions were 2.38 (95%CI:1.60-3.53) times higher than those who received leukodepleted TC. Non-leukodepleted have significant higher risks of increased CD62P expression than leukodepleted  TC transfusions.
      PubDate: 2017-03-03
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