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Showing 1 - 200 of 3043 Journals sorted alphabetically
AASRI Procedia     Open Access   (Followers: 15)
Academic Pediatrics     Hybrid Journal   (Followers: 22, SJR: 1.402, h-index: 51)
Academic Radiology     Hybrid Journal   (Followers: 21, SJR: 1.008, h-index: 75)
Accident Analysis & Prevention     Partially Free   (Followers: 84, SJR: 1.109, h-index: 94)
Accounting Forum     Hybrid Journal   (Followers: 25, SJR: 0.612, h-index: 27)
Accounting, Organizations and Society     Hybrid Journal   (Followers: 30, SJR: 2.515, h-index: 90)
Achievements in the Life Sciences     Open Access   (Followers: 4)
Acta Anaesthesiologica Taiwanica     Open Access   (Followers: 5, SJR: 0.338, h-index: 19)
Acta Astronautica     Hybrid Journal   (Followers: 351, SJR: 0.726, h-index: 43)
Acta Automatica Sinica     Full-text available via subscription   (Followers: 3)
Acta Biomaterialia     Hybrid Journal   (Followers: 25, SJR: 2.02, h-index: 104)
Acta Colombiana de Cuidado Intensivo     Full-text available via subscription   (Followers: 1)
Acta de Investigación Psicológica     Open Access   (Followers: 2)
Acta Ecologica Sinica     Open Access   (Followers: 8, SJR: 0.172, h-index: 29)
Acta Haematologica Polonica     Free   (SJR: 0.123, h-index: 8)
Acta Histochemica     Hybrid Journal   (Followers: 3, SJR: 0.604, h-index: 38)
Acta Materialia     Hybrid Journal   (Followers: 238, SJR: 3.683, h-index: 202)
Acta Mathematica Scientia     Full-text available via subscription   (Followers: 5, SJR: 0.615, h-index: 21)
Acta Mechanica Solida Sinica     Full-text available via subscription   (Followers: 9, SJR: 0.442, h-index: 21)
Acta Oecologica     Hybrid Journal   (Followers: 10, SJR: 0.915, h-index: 53)
Acta Otorrinolaringologica (English Edition)     Full-text available via subscription   (Followers: 1)
Acta Otorrinolaringológica Española     Full-text available via subscription   (Followers: 3, SJR: 0.311, h-index: 16)
Acta Pharmaceutica Sinica B     Open Access   (Followers: 2)
Acta Poética     Open Access   (Followers: 4)
Acta Psychologica     Hybrid Journal   (Followers: 23, SJR: 1.365, h-index: 73)
Acta Sociológica     Open Access  
Acta Tropica     Hybrid Journal   (Followers: 6, SJR: 1.059, h-index: 77)
Acta Urológica Portuguesa     Open Access  
Actas Dermo-Sifiliograficas     Full-text available via subscription   (Followers: 4)
Actas Dermo-Sifiliográficas (English Edition)     Full-text available via subscription   (Followers: 3)
Actas Urológicas Españolas     Full-text available via subscription   (Followers: 4, SJR: 0.383, h-index: 19)
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Actualites Pharmaceutiques     Full-text available via subscription   (Followers: 5, SJR: 0.141, h-index: 3)
Actualites Pharmaceutiques Hospitalieres     Full-text available via subscription   (Followers: 4, SJR: 0.112, h-index: 2)
Acupuncture and Related Therapies     Hybrid Journal   (Followers: 4)
Acute Pain     Full-text available via subscription   (Followers: 13)
Ad Hoc Networks     Hybrid Journal   (Followers: 11, SJR: 0.967, h-index: 57)
Addictive Behaviors     Hybrid Journal   (Followers: 15, SJR: 1.514, h-index: 92)
Addictive Behaviors Reports     Open Access   (Followers: 5)
Additive Manufacturing     Hybrid Journal   (Followers: 7, SJR: 1.039, h-index: 5)
Additives for Polymers     Full-text available via subscription   (Followers: 21)
Advanced Drug Delivery Reviews     Hybrid Journal   (Followers: 135, SJR: 5.2, h-index: 222)
Advanced Engineering Informatics     Hybrid Journal   (Followers: 11, SJR: 1.265, h-index: 53)
Advanced Powder Technology     Hybrid Journal   (Followers: 17, SJR: 0.739, h-index: 33)
Advances in Accounting     Hybrid Journal   (Followers: 9, SJR: 0.299, h-index: 15)
Advances in Agronomy     Full-text available via subscription   (Followers: 15, SJR: 2.071, h-index: 82)
Advances in Anesthesia     Full-text available via subscription   (Followers: 25, SJR: 0.169, h-index: 4)
Advances in Antiviral Drug Design     Full-text available via subscription   (Followers: 3)
Advances in Applied Mathematics     Full-text available via subscription   (Followers: 6, SJR: 1.054, h-index: 35)
Advances in Applied Mechanics     Full-text available via subscription   (Followers: 11, SJR: 0.801, h-index: 26)
Advances in Applied Microbiology     Full-text available via subscription   (Followers: 22, SJR: 1.286, h-index: 49)
Advances In Atomic, Molecular, and Optical Physics     Full-text available via subscription   (Followers: 16, SJR: 3.31, h-index: 42)
Advances in Biological Regulation     Hybrid Journal   (Followers: 4, SJR: 2.277, h-index: 43)
Advances in Botanical Research     Full-text available via subscription   (Followers: 3, SJR: 0.619, h-index: 48)
Advances in Cancer Research     Full-text available via subscription   (Followers: 25, SJR: 2.215, h-index: 78)
Advances in Carbohydrate Chemistry and Biochemistry     Full-text available via subscription   (Followers: 9, SJR: 0.9, h-index: 30)
Advances in Catalysis     Full-text available via subscription   (Followers: 5, SJR: 2.139, h-index: 42)
Advances in Cellular and Molecular Biology of Membranes and Organelles     Full-text available via subscription   (Followers: 12)
Advances in Chemical Engineering     Full-text available via subscription   (Followers: 26, SJR: 0.183, h-index: 23)
Advances in Child Development and Behavior     Full-text available via subscription   (Followers: 10, SJR: 0.665, h-index: 29)
Advances in Chronic Kidney Disease     Full-text available via subscription   (Followers: 9, SJR: 1.268, h-index: 45)
Advances in Clinical Chemistry     Full-text available via subscription   (Followers: 29, SJR: 0.938, h-index: 33)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 18, SJR: 2.314, h-index: 130)
Advances in Computers     Full-text available via subscription   (Followers: 16, SJR: 0.223, h-index: 22)
Advances in Dermatology     Full-text available via subscription   (Followers: 12)
Advances in Developmental Biology     Full-text available via subscription   (Followers: 11)
Advances in Digestive Medicine     Open Access   (Followers: 6)
Advances in DNA Sequence-Specific Agents     Full-text available via subscription   (Followers: 5)
Advances in Drug Research     Full-text available via subscription   (Followers: 22)
Advances in Ecological Research     Full-text available via subscription   (Followers: 41, SJR: 3.25, h-index: 43)
Advances in Engineering Software     Hybrid Journal   (Followers: 25, SJR: 0.486, h-index: 10)
Advances in Experimental Biology     Full-text available via subscription   (Followers: 7)
Advances in Experimental Social Psychology     Full-text available via subscription   (Followers: 41, SJR: 5.465, h-index: 64)
Advances in Exploration Geophysics     Full-text available via subscription   (Followers: 3)
Advances in Food and Nutrition Research     Full-text available via subscription   (Followers: 50, SJR: 0.674, h-index: 38)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 16)
Advances in Genetics     Full-text available via subscription   (Followers: 15, SJR: 2.558, h-index: 54)
Advances in Genome Biology     Full-text available via subscription   (Followers: 11)
Advances in Geophysics     Full-text available via subscription   (Followers: 6, SJR: 2.325, h-index: 20)
Advances in Heat Transfer     Full-text available via subscription   (Followers: 22, SJR: 0.906, h-index: 24)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 9, SJR: 0.497, h-index: 31)
Advances in Imaging and Electron Physics     Full-text available via subscription   (Followers: 2, SJR: 0.396, h-index: 27)
Advances in Immunology     Full-text available via subscription   (Followers: 35, SJR: 4.152, h-index: 85)
Advances in Inorganic Chemistry     Full-text available via subscription   (Followers: 9, SJR: 1.132, h-index: 42)
Advances in Insect Physiology     Full-text available via subscription   (Followers: 3, SJR: 1.274, h-index: 27)
Advances in Integrative Medicine     Hybrid Journal   (Followers: 6)
Advances in Life Course Research     Hybrid Journal   (Followers: 8, SJR: 0.764, h-index: 15)
Advances in Lipobiology     Full-text available via subscription   (Followers: 2)
Advances in Magnetic and Optical Resonance     Full-text available via subscription   (Followers: 9)
Advances in Marine Biology     Full-text available via subscription   (Followers: 16, SJR: 1.645, h-index: 45)
Advances in Mathematics     Full-text available via subscription   (Followers: 10, SJR: 3.261, h-index: 65)
Advances in Medical Sciences     Hybrid Journal   (Followers: 6, SJR: 0.489, h-index: 25)
Advances in Medicinal Chemistry     Full-text available via subscription   (Followers: 5)
Advances in Microbial Physiology     Full-text available via subscription   (Followers: 4, SJR: 1.44, h-index: 51)
Advances in Molecular and Cell Biology     Full-text available via subscription   (Followers: 22)
Advances in Molecular and Cellular Endocrinology     Full-text available via subscription   (Followers: 10)
Advances in Molecular Toxicology     Full-text available via subscription   (Followers: 7, SJR: 0.324, h-index: 8)
Advances in Nanoporous Materials     Full-text available via subscription   (Followers: 4)
Advances in Oncobiology     Full-text available via subscription   (Followers: 3)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 15, SJR: 2.885, h-index: 45)
Advances in Parallel Computing     Full-text available via subscription   (Followers: 7, SJR: 0.148, h-index: 11)
Advances in Parasitology     Full-text available via subscription   (Followers: 7, SJR: 2.37, h-index: 73)
Advances in Pediatrics     Full-text available via subscription   (Followers: 24, SJR: 0.4, h-index: 28)
Advances in Pharmaceutical Sciences     Full-text available via subscription   (Followers: 13)
Advances in Pharmacology     Full-text available via subscription   (Followers: 15, SJR: 1.718, h-index: 58)
Advances in Physical Organic Chemistry     Full-text available via subscription   (Followers: 8, SJR: 0.384, h-index: 26)
Advances in Phytomedicine     Full-text available via subscription  
Advances in Planar Lipid Bilayers and Liposomes     Full-text available via subscription   (Followers: 3, SJR: 0.248, h-index: 11)
Advances in Plant Biochemistry and Molecular Biology     Full-text available via subscription   (Followers: 8)
Advances in Plant Pathology     Full-text available via subscription   (Followers: 5)
Advances in Porous Media     Full-text available via subscription   (Followers: 4)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 17)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 20, SJR: 1.5, h-index: 62)
Advances in Psychology     Full-text available via subscription   (Followers: 61)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 5, SJR: 0.478, h-index: 32)
Advances in Radiation Oncology     Open Access  
Advances in Small Animal Medicine and Surgery     Hybrid Journal   (Followers: 3, SJR: 0.1, h-index: 2)
Advances in Space Research     Full-text available via subscription   (Followers: 353, SJR: 0.606, h-index: 65)
Advances in Structural Biology     Full-text available via subscription   (Followers: 8)
Advances in Surgery     Full-text available via subscription   (Followers: 7, SJR: 0.823, h-index: 27)
Advances in the Study of Behavior     Full-text available via subscription   (Followers: 30, SJR: 1.321, h-index: 56)
Advances in Veterinary Medicine     Full-text available via subscription   (Followers: 17)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 13)
Advances in Virus Research     Full-text available via subscription   (Followers: 5, SJR: 1.878, h-index: 68)
Advances in Water Resources     Hybrid Journal   (Followers: 43, SJR: 2.408, h-index: 94)
Aeolian Research     Hybrid Journal   (Followers: 5, SJR: 0.973, h-index: 22)
Aerospace Science and Technology     Hybrid Journal   (Followers: 325, SJR: 0.816, h-index: 49)
AEU - Intl. J. of Electronics and Communications     Hybrid Journal   (Followers: 8, SJR: 0.318, h-index: 36)
African J. of Emergency Medicine     Open Access   (Followers: 5, SJR: 0.344, h-index: 6)
Ageing Research Reviews     Hybrid Journal   (Followers: 8, SJR: 3.289, h-index: 78)
Aggression and Violent Behavior     Hybrid Journal   (Followers: 405, SJR: 1.385, h-index: 72)
Agri Gene     Hybrid Journal  
Agricultural and Forest Meteorology     Hybrid Journal   (Followers: 15, SJR: 2.18, h-index: 116)
Agricultural Systems     Hybrid Journal   (Followers: 30, SJR: 1.275, h-index: 74)
Agricultural Water Management     Hybrid Journal   (Followers: 39, SJR: 1.546, h-index: 79)
Agriculture and Agricultural Science Procedia     Open Access  
Agriculture and Natural Resources     Open Access   (Followers: 1)
Agriculture, Ecosystems & Environment     Hybrid Journal   (Followers: 54, SJR: 1.879, h-index: 120)
Ain Shams Engineering J.     Open Access   (Followers: 5, SJR: 0.434, h-index: 14)
Air Medical J.     Hybrid Journal   (Followers: 5, SJR: 0.234, h-index: 18)
AKCE Intl. J. of Graphs and Combinatorics     Open Access   (SJR: 0.285, h-index: 3)
Alcohol     Hybrid Journal   (Followers: 10, SJR: 0.922, h-index: 66)
Alcoholism and Drug Addiction     Open Access   (Followers: 8)
Alergologia Polska : Polish J. of Allergology     Full-text available via subscription   (Followers: 1)
Alexandria Engineering J.     Open Access   (Followers: 1, SJR: 0.436, h-index: 12)
Alexandria J. of Medicine     Open Access  
Algal Research     Partially Free   (Followers: 8, SJR: 2.05, h-index: 20)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 3)
Allergologia et Immunopathologia     Full-text available via subscription   (Followers: 1, SJR: 0.46, h-index: 29)
Allergology Intl.     Open Access   (Followers: 4, SJR: 0.776, h-index: 35)
Alpha Omegan     Full-text available via subscription   (SJR: 0.121, h-index: 9)
ALTER - European J. of Disability Research / Revue Européenne de Recherche sur le Handicap     Full-text available via subscription   (Followers: 8, SJR: 0.158, h-index: 9)
Alzheimer's & Dementia     Hybrid Journal   (Followers: 48, SJR: 4.289, h-index: 64)
Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring     Open Access   (Followers: 6)
Alzheimer's & Dementia: Translational Research & Clinical Interventions     Open Access   (Followers: 5)
American Heart J.     Hybrid Journal   (Followers: 49, SJR: 3.157, h-index: 153)
American J. of Cardiology     Hybrid Journal   (Followers: 47, SJR: 2.063, h-index: 186)
American J. of Emergency Medicine     Hybrid Journal   (Followers: 39, SJR: 0.574, h-index: 65)
American J. of Geriatric Pharmacotherapy     Full-text available via subscription   (Followers: 8, SJR: 1.091, h-index: 45)
American J. of Geriatric Psychiatry     Hybrid Journal   (Followers: 15, SJR: 1.653, h-index: 93)
American J. of Human Genetics     Hybrid Journal   (Followers: 31, SJR: 8.769, h-index: 256)
American J. of Infection Control     Hybrid Journal   (Followers: 25, SJR: 1.259, h-index: 81)
American J. of Kidney Diseases     Hybrid Journal   (Followers: 32, SJR: 2.313, h-index: 172)
American J. of Medicine     Hybrid Journal   (Followers: 45, SJR: 2.023, h-index: 189)
American J. of Medicine Supplements     Full-text available via subscription   (Followers: 3)
American J. of Obstetrics and Gynecology     Hybrid Journal   (Followers: 235, SJR: 2.255, h-index: 171)
American J. of Ophthalmology     Hybrid Journal   (Followers: 57, SJR: 2.803, h-index: 148)
American J. of Ophthalmology Case Reports     Open Access   (Followers: 5)
American J. of Orthodontics and Dentofacial Orthopedics     Full-text available via subscription   (Followers: 6, SJR: 1.249, h-index: 88)
American J. of Otolaryngology     Hybrid Journal   (Followers: 25, SJR: 0.59, h-index: 45)
American J. of Pathology     Hybrid Journal   (Followers: 26, SJR: 2.653, h-index: 228)
American J. of Preventive Medicine     Hybrid Journal   (Followers: 22, SJR: 2.764, h-index: 154)
American J. of Surgery     Hybrid Journal   (Followers: 34, SJR: 1.286, h-index: 125)
American J. of the Medical Sciences     Hybrid Journal   (Followers: 12, SJR: 0.653, h-index: 70)
Ampersand : An Intl. J. of General and Applied Linguistics     Open Access   (Followers: 5)
Anaerobe     Hybrid Journal   (Followers: 4, SJR: 1.066, h-index: 51)
Anaesthesia & Intensive Care Medicine     Full-text available via subscription   (Followers: 57, SJR: 0.124, h-index: 9)
Anaesthesia Critical Care & Pain Medicine     Full-text available via subscription   (Followers: 11)
Anales de Cirugia Vascular     Full-text available via subscription  
Anales de Pediatría     Full-text available via subscription   (Followers: 2, SJR: 0.209, h-index: 27)
Anales de Pediatría (English Edition)     Full-text available via subscription  
Anales de Pediatría Continuada     Full-text available via subscription   (SJR: 0.104, h-index: 3)
Analytic Methods in Accident Research     Hybrid Journal   (Followers: 4, SJR: 2.577, h-index: 7)
Analytica Chimica Acta     Hybrid Journal   (Followers: 37, SJR: 1.548, h-index: 152)
Analytical Biochemistry     Hybrid Journal   (Followers: 167, SJR: 0.725, h-index: 154)
Analytical Chemistry Research     Open Access   (Followers: 8, SJR: 0.18, h-index: 2)
Analytical Spectroscopy Library     Full-text available via subscription   (Followers: 11)
Anesthésie & Réanimation     Full-text available via subscription   (Followers: 1)
Anesthesiology Clinics     Full-text available via subscription   (Followers: 22, SJR: 0.421, h-index: 40)
Angiología     Full-text available via subscription   (SJR: 0.124, h-index: 9)
Angiologia e Cirurgia Vascular     Open Access  
Animal Behaviour     Hybrid Journal   (Followers: 161, SJR: 1.907, h-index: 126)
Animal Feed Science and Technology     Hybrid Journal   (Followers: 5, SJR: 1.151, h-index: 83)
Animal Reproduction Science     Hybrid Journal   (Followers: 5, SJR: 0.711, h-index: 78)
Annales d'Endocrinologie     Full-text available via subscription   (Followers: 1, SJR: 0.394, h-index: 30)
Annales d'Urologie     Full-text available via subscription  
Annales de Cardiologie et d'Angéiologie     Full-text available via subscription   (SJR: 0.177, h-index: 13)
Annales de Chirurgie de la Main et du Membre Supérieur     Full-text available via subscription  
Annales de Chirurgie Plastique Esthétique     Full-text available via subscription   (Followers: 2, SJR: 0.354, h-index: 22)
Annales de Chirurgie Vasculaire     Full-text available via subscription   (Followers: 1)

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Journal Cover Animal Reproduction Science
  [SJR: 0.711]   [H-I: 78]   [5 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0378-4320
   Published by Elsevier Homepage  [3043 journals]
  • Supplementation of cilostazol during in vitro maturation enhances the
           meiosis and developmental competence of yak oocytes by influencing cAMP
           content and mRNA expression
    • Authors: Xian-Rong Xiong; Dao-Liang Lan; Jian Li; Ya-Qiu Lin; Ming-Yang Li
      Abstract: Publication date: November 2017
      Source:Animal Reproduction Science, Volume 186
      Author(s): Xian-Rong Xiong, Dao-Liang Lan, Jian Li, Ya-Qiu Lin, Ming-Yang Li
      The efficiency of in vitro embryo production remains low compared with that observed in vivo. Recent studies have independently shown that cyclic adenosine monophosphate (cAMP) modulation prior to in vitro maturation (IVM) supplementation improves oocyte developmental competence. In this context, special cAMP modulators have been applied during IVM as promising alternatives to improve this biotechnology. Accordingly, this study was conducted to evaluate the effects of treatment with cilostazol, a PDE3 inhibitor, during pre-IVM culture on oocyte meiotic maturation in yak. Immature yak cumulus-oocyte complexes (COCs) were treated in vitro without (control) or with 5μM cilostazol for 0, 2, or 4h prior to IVM. Results showed that the presence of cilostazol in pre-IVM medium significantly increased the percentages of oocytes at metaphase II stage compared with that in the control groups (P< 0.05). Moreover, pre-IVM with cilostazol significantly enhanced intraoocyte cAMP and glutathione (GSH) levels at the pre-IVM or IVM phase relative to the no pre-IVM groups (P< 0.05). After in vitro fertilization (IVF) and parthenogenetic activation (PA), the developmental competences of oocytes and embryo quality were improved significantly after pre-IVM with cilostazol compared with the control groups (P< 0.05), given that the cleavage and blastocyst formation rates and the total number of blastocyst cells were increased. The presence of cilostazol also increased the levels of mRNA expression for adenylate cyclase 3 (ADCY3) and protein kinase 1 (PKA1), as well as decreased the abundance of phosphodiesterase 3A (PDE3A) in COCs and IVF blastocysts, compared with their control counterparts (P< 0.05). The results demonstrated that the meiotic progression of immature yak oocytes could be reversibly affected by cAMP modulators. By contrast, treatment with cilostazol during pre-IVM positively affected the developmental competence of yak oocytes, probably by improving intraoocyte cAMP and GSH levels and regulating mRNA expression patterns. We concluded that appropriate treatment with cilostazol during pre-IVM would be beneficial for oocyte maturation in vitro.

      PubDate: 2017-10-11T20:05:08Z
      DOI: 10.1016/j.anireprosci.2017.08.013
      Issue No: Vol. 186 (2017)
  • Different approaches to establish infertile rooster
    • Authors: Fereshteh Ghadimi; Malak Shakeri; Mahdi Zhandi; Mojtaba Zaghari; Abbas Piryaei; Parham Moslehifar; Alireza Rajabinejad
      Abstract: Publication date: November 2017
      Source:Animal Reproduction Science, Volume 186
      Author(s): Fereshteh Ghadimi, Malak Shakeri, Mahdi Zhandi, Mojtaba Zaghari, Abbas Piryaei, Parham Moslehifar, Alireza Rajabinejad
      Several methods have been developed to suppress spermatogenesis in recipient males before spermatogonial stem cells (SSCs) transplantation. The aim of this study was to compare two different methods of depleting endogenous spermatogenesis in recipient ROSS 308 strain adult roosters. Gamma-radiation and alkylating agent busulfan were utilized to infertilize adult roosters (ROSS 308 strain). Two radiation therapy regimes (based on 60co isotope) were conducted locally to testes using 40Gy (5×8Gy with three-day intervals) and 30Gy (3×10Gy with three-day intervals). And two different levels of busulfan 60mg(40+20) and 50mg(30+20) with 10-day intervals were injected intraperitoneally. The results showed that both radiation therapy regimes and both busulfan levels reduced sperm motility and sperm concentration significantly compared with control group. Moreover, there were no significant differences between gamma radiation and busulfan treatments in progressive and total motility of sperm reduction. Sperm concentration reached to zero at the end of the 4th week of experiment in all treatment groups. Also histological examinations revealed that both treatments could significantly reduce the diameter of seminiferous tubules and thickness of epithelium. None of the treatments had significant effect on body weight in comparison with control group and the health status of experimental roosters remained good throughout the study. Given that, the risk probability of high doses of radiation exposure and busulfan, it can be concluded that the 30Gy (3×10Gy) and 50mg (30+20) are appropriate for suppression of endogenous spermatogenesis in mature roosters.

      PubDate: 2017-10-11T20:05:08Z
      DOI: 10.1016/j.anireprosci.2017.08.019
      Issue No: Vol. 186 (2017)
  • Effect of kisspeptin on the proliferation and apoptosis of bovine
           granulosa cells
    • Authors: Hongyu Liu; Gaoqing Xu; Zhiyu Yuan; Yangyunyi Dong; Jun Wang; Wenfa Lu
      Pages: 1 - 7
      Abstract: Publication date: October 2017
      Source:Animal Reproduction Science, Volume 185
      Author(s): Hongyu Liu, Gaoqing Xu, Zhiyu Yuan, Yangyunyi Dong, Jun Wang, Wenfa Lu
      Previous studies have shown that kisspeptin (Kp-10) is expressed in mammalian ovaries; however, the expression and role of Kp-10 in bovine ovarian granulosa cells are still unclear. In this study, we assessed the expression of Kp-10 and its effects on the proliferation and apoptosis of bovine granulosa cells. Immunohistochemical analysis showed that Kp-10 was expressed in the cytoplasm of bovine ovarian granulosa cells. Moreover, MTT assays showed that 100nM Kp-10 significantly inhibited the viability of granulosa cells (P< 0.05). Flow cytometry analysis showed that Kp-10 could significantly increase accumulation of cells in the G1 phase, decrease accumulation of cells in the S phase, and promote apoptosis in bovine granulosa cells (P< 0.05). Additionally, Kp-10 decreased the mRNA levels of Bcl-2, an anti-apoptotic gene; increased the mRNA levels of caspase-3, a pro-apoptotic gene; and increased the mRNA levels of Fas and Fasl (P<  0.05). Thus, our findings demonstrated for the first time that Kp-10 inhibited proliferation and promoted apoptosis in bovine ovarian granulosa cells. These findings provide insights into our understanding of the role of Kp-10 in mediating the proliferation of bovine granulosa cells.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.07.008
      Issue No: Vol. 185 (2017)
  • Divergently expressed gene identification and interaction prediction of
           long noncoding RNA and mRNA involved in duck reproduction
    • Authors: Jindong Ren; Xue Du; Tao Zeng; Li Chen; Junda Shen; Lizhi Lu; Jianhong Hu
      Pages: 8 - 17
      Abstract: Publication date: October 2017
      Source:Animal Reproduction Science, Volume 185
      Author(s): Jindong Ren, Xue Du, Tao Zeng, Li Chen, Junda Shen, Lizhi Lu, Jianhong Hu
      Long noncoding RNAs (lncRNAs) and divergently expressed genes exist widely in different tissues of mammals and birds, in which they are involved in various biological processes. However, there is limited information on their role in the regulation of normal biological processes during differentiation, development, and reproduction in birds. In this study, whole transcriptome strand-specific RNA sequencing of the ovary from young ducks (60days), first-laying ducks (160days), and old ducks, i.e., ducks that stopped laying eggs (490days) was performed. The lncRNAs and mRNAs from these ducks were systematically analyzed and identified by duck genome sequencing in the three study groups. The transcriptome from the duck ovary comprised 15,011 protein-coding genes and 2905 lncRNAs; all the lncRNAs were identified as novel long noncoding transcripts. The comparison of transcriptome data from different study groups identified 2240 divergent transcription genes and 135 divergently expressed lncRNAs, which differed among the groups; most of them were significantly downregulated with age. Among the divergent genes, 38 genes were related to the reproductive process and 6 genes were upregulated. Further prediction analysis revealed that 52 lncRNAs were closely correlated with divergent reproductive mRNAs. More importantly, 6 remarkable lncRNAs were correlated significantly with the conversion of the ovary in different phases. Our results aid in the understanding of the divergent transcriptome of duck ovary in different phases and the underlying mechanisms that drive the specificity of protein-coding genes and lncRNAs in duck ovary.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.07.012
      Issue No: Vol. 185 (2017)
  • Effects of sodium pyruvate on viability, synthesis of reactive oxygen
           species, lipid peroxidation and DNA integrity of cryopreserved bovine
    • Authors: F. Korkmaz; E. Malama; M. Siuda; C. Leiding; H. Bollwein
      Pages: 18 - 27
      Abstract: Publication date: October 2017
      Source:Animal Reproduction Science, Volume 185
      Author(s): F. Korkmaz, E. Malama, M. Siuda, C. Leiding, H. Bollwein
      The aim of this study was to examine effects of sodium pyruvate on viability as well as on synthesis of reactive oxygen species (ROS), lipid peroxidation and DNA integrity of cryopreserved bovine sperm. In each of 23 Simmental AI bulls three ejaculates were collected. In a split sample design ejaculates were diluted by using Triladyl® extender without and with the addition of 5mM sodium pyruvate. Both aliquots were equilibrated for 24h before freezing. Frozen sperm samples were thawed, and examined immediately after thawing (0h) as well as after 3, 6, 12, and 24h incubation at 37°C. The percentages of rapidly motile sperm (RMS), plasma membrane and acrosome intact sperm (PMAI), sperm with a high mitochondrial membrane potential (HMMP), amounts of ROS synthesis (dichlorofluorescein-diacetate (DCFH), CellROX Deep Red Reagent® probe (CellROX)) and lipid peroxidation of sperm (LPO) and percentage of sperm with a high degree of DNA fragmentation (%DFI) were determined. Overall, sperm diluted with the extender containing sodium pyruvate showed higher levels of RMS, PMAI and HMMP, CellROX and lower %DFI values (P<0.001) compared to sperm frozen in the extender without sodium pyruvate. However, there was no effect (P>0.05) of sodium pyruvate on LPO and DCFH. The results of this study show that the addition of sodium pyruvate to the semen extender improved the viability as well as DNA integrity of cryopreserved sperm and did not affect their lipid peroxidation, although it increased the synthesis of some ROS.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.07.017
      Issue No: Vol. 185 (2017)
  • Eggshell matrix proteins OC-116, OC-17 and OCX36 in hen's sperm storage
    • Authors: Cindy Riou; Luiz Cordeiro; Nadine Gérard
      Pages: 28 - 41
      Abstract: Publication date: October 2017
      Source:Animal Reproduction Science, Volume 185
      Author(s): Cindy Riou, Luiz Cordeiro, Nadine Gérard
      While uterine epithelium secretes eggshell matrix proteins to regulate eggshell structural organization, uterovaginal junction (UVJ) epithelium supports sperm storage in tubules (SST). Here, we examined the presence of OCX36, OC-116 and OC-17 eggshell matrix proteins in SSTs. Two experimental lines of hens displaying either a long (F+ line) or a short (F- line) potential to store sperm were used, before and 24h after insemination. Using immunohistochemistry and western blot, we analyzed the presence of OC-116, OC-17 and OCX36 proteins in the SSTs. Using lectin and calcium staining, we examined the presence in SSTs of Gal/GalNAc (Galactose/N-acetylgalactosamine) and Glc/GlcNAc (Glucose/N-acetylglucosamine) glycans, as well as calcium ions. Our results indicate that in both F+ and F- hens, the eggshell matrix proteins OC-116 and OCX36 were identified in SST cells and lumen, in contact with spermatozoa. The OC-17 protein was found associated with calcium in F+ and F- hens, only in the SST lumen 24h after insemination. Glycans Gal/GalNAc and Glc/GlcNAc were found to be more abundant in the apical cytoplasmic area of the SST cells of F+ hens than in that of F- hens after insemination. This is the first report demonstrating the presence in SSTs of the OC-116, OC-17 and OCX36 eggshell matrix proteins, and their concomitant presence with Gal/GalNAc and Glc/GlcNAc glycans, as well as with calcium. Our results suggest that the OC-116, OC-17 and OCX36 eggshell matrix proteins may be involved in sperm storage.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.07.022
      Issue No: Vol. 185 (2017)
  • Sperm quality variables as indicators of bull fertility may be breed
    • Authors: Jane M Morrell; Thanapol Nongbua; Sabina Valeanu; Isabel Lima Verde; Katrin Lundstedt-Enkel; Anders Edman; Anders Johannisson
      Pages: 42 - 52
      Abstract: Publication date: October 2017
      Source:Animal Reproduction Science, Volume 185
      Author(s): Jane M Morrell, Thanapol Nongbua, Sabina Valeanu, Isabel Lima Verde, Katrin Lundstedt-Enkel, Anders Edman, Anders Johannisson
      A means of discriminating among bulls of high fertility based on sperm quality is needed by breeding centers. The objective of the study was to examine parameters of sperm quality in bulls of known fertility to identify useful indicators of fertility. Frozen semen was available from bulls of known fertility (Viking Genetics, Skara, Sweden): Swedish Red (n =31), Holstein (n =25) and Others (one each of Charolais, Limousin, Blonde, SKB). After thawing, the sperm samples were analyzed for motility (computer assisted sperm analysis), plasma membrane integrity, chromatin integrity, acrosome status, mitochondrial activity and reactive oxygen species. A fertility index score based on the adjusted 56-day non-return rate for >1000 inseminations was available for each bull. Multivariate data analysis (Partial Least Squares Regression and Orthogonal Partial Least Squares Regression) was performed to identify variables related to fertility; Pearson univariate correlations were made on the parameters of interest. Breed of bull affected the relationship of sperm quality variables and fertility index score, as follows: Swedish Red: %DNA Fragmentation Index, r =−0.56, P<0.01; intact plasma membrane, r =0.40, P< 0.05; membrane damaged, not acrosome reacted, r =−0.6, P< 0.01; Linearity, r =0.37, P< 0.05; there was a trend towards significance for Wobble, r =0.34, P =0.08. Holstein: Linearity was significant r =0.46, P< 0.05; there was a trend towards significance for Wobble, r =0.45, P =0.08. In conclusion, breed has a greater effect on sperm quality than previously realized; different parameters of sperm quality are needed to indicate potential fertility in different breeds.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.08.001
      Issue No: Vol. 185 (2017)
  • Comparison of different methods of semen cryopreservation in Melopsittacus
    • Authors: Andrea Dogliero; Mitzy Mauthe von Degerfeld; Ada Rota; Paola Pregel; Giuseppe Quaranta
      Pages: 53 - 65
      Abstract: Publication date: October 2017
      Source:Animal Reproduction Science, Volume 185
      Author(s): Andrea Dogliero, Mitzy Mauthe von Degerfeld, Ada Rota, Paola Pregel, Giuseppe Quaranta
      Melopsittacus undulatus is largely used as a potential model for assisted reproduction in other endangered parrot species. Semen was collected from nineteen healthy adult males, by massage technique, during the breeding season. After preliminary evaluations through CASA analysis, eight birds were selected as semen donors and five ejaculates of each bird were utilized to evaluate the effect of 10% ethylene glycol (EG) addition, in two times, during 40min of equilibration time at 4°C, and to compare two programmable freezing curves (a rapid and a slow temperature descent rate) and freezing on nitrogen vapors. Diluted semen (modified TALP, pH 8.4) was divided into two aliquots, the first for freezing individual samples, the second to be mixed in a semen pool from the eight birds. Potential inseminating doses of 10μl, containing 1×106 spermatozoa, were frozen. The effect of EG addition on semen motility and kinetic parameters was analyzed and the three freezing methods were compared. EG addition caused a significant decline of semen motility in individual samples, not in semen pools. The three freezing curves resulted in significant differences in thawed-semen parameters, with nitrogen vapors showing the worst results, while the higher total and progressive motility values were obtained with the ‘fast’ protocol. Thawed-semen pools motility and kinetic parameters appeared lower than those of the individual ejaculates. The differences found between single ejaculates and semen pools emphasize the importance of performing artificial insemination tests in order to assess the in vivo performance of single ejaculates in the parrot species.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.08.002
      Issue No: Vol. 185 (2017)
  • ROS activates autophagy in follicular granulosa cells via mTOR pathway to
           regulate broodiness in goose
    • Authors: Yaping Lou; Wensai Yu; Lu Han; Songbai Yang; Yali Wang; Ting Ren; Jing Yu; Ayong Zhao
      Pages: 97 - 103
      Abstract: Publication date: October 2017
      Source:Animal Reproduction Science, Volume 185
      Author(s): Yaping Lou, Wensai Yu, Lu Han, Songbai Yang, Yali Wang, Ting Ren, Jing Yu, Ayong Zhao
      Broodiness causes reduced reproductive ability in poultry, but its regulatory mechanism remains poorly understood. ROS (reactive oxygen species) and autophagy are important for follicular development, and the interaction between the two may play a role in regulating broodiness. We examined goose follicles for ROS and oxidation scavenger activities during the egg-laying and broody stages. The follicular granulosa cells were exposed to media containing H2O2, and the interactions between ROS and autophagy in follicular granulosa cells in vitro were analyzed using a Western blot method. We found that the activities of superoxide dismutase (SOD) and lactate dehydrogenase (LDH) were enhanced and the amount of malondialdehyde (MDA) decreased in broody goose follicles. H2O2 inhibited the cell viability and induced autophagy. Furthermore, it was also found that H2O2 regulated autophagy by reducing mTOR and increasing p53; however, H2O2 had no impact on Beclin1 or ATG12. It was also shown that the enhanced autophagy lessened ROS-induced damages. We conclude that ROS and autophagy both played important roles in regulating follicular development to control broodiness in geese, and ROS activated autophagy in follicular granulosa cells via the mTOR pathway.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.08.008
      Issue No: Vol. 185 (2017)
  • Effect of feeding three lysine to energy diets on growth, body composition
           and age at puberty in replacement gilts
    • Authors: J.A. Calderón Díaz; J.L. Vallet; R.D. Boyd; C.A. Lents; T.J. Prince; A.E. DeDecker; C.E. Phillips; G. Foxcroft; K.J. Stalder
      Pages: 1 - 10
      Abstract: Publication date: September 2017
      Source:Animal Reproduction Science, Volume 184
      Author(s): J.A. Calderón Díaz, J.L. Vallet, R.D. Boyd, C.A. Lents, T.J. Prince, A.E. DeDecker, C.E. Phillips, G. Foxcroft, K.J. Stalder
      This study evaluated the effect of diets differing in standard ileal digestible (SID) lysine on lysine intake, growth rate, body composition and age at puberty on maternal line gilts. Crossbred Large White×Landrace gilts (n =641) were fed corn-soybean diets differing in SID lysine concentration (%, g SID lysine:Mcal ME); diets were not isocaloric. Gilts received three grower, finisher diet combinations: low (0.68% lysine grower, 0.52% lysine finisher), medium (0.79% lysine grower, 0.60% lysine finisher) or high (0.90% lysine grower, 0.68% lysine finisher). Grower diets were fed from 100 until 142days of age, and finisher diets were fed until they reached 220days of age. Body weight (BW), backfat thickness (BF), and loin depth (LD) were recorded every 28days. From 160–220days of age, gilts were exposed daily to vasectomized boars and observed for behavioral estrus. Gilts fed the low lysine diet had lower average daily gain and BW (P< 0.05), but not fat depth:LD ratio. The percentage of gilts that displayed natural estrus by 220days of age was low but not different among dietary treatments (low 27.7%, medium 31.0% and high 37.7%, respectively; P =0.1201). Gilts fed the high and medium diets reached puberty 10 and 6days earlier, however, than gilts fed the low lysine diet (P <0.05). The rate of puberty attainment may have been less because all gilts contracted porcine epidemic diarrhea (PEDv) just as boar exposure was to begin for the first group of gilts. Results from the present study indicate that growth rate and age at puberty can be altered by ad libitum fed diets that differ in SID lysine concentration.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.06.007
      Issue No: Vol. 184 (2017)
  • Supplementation of tris-based extender with plasma egg yolk of six avian
           species and camel skim milk for chilled preservation of dromedary camel
    • Authors: Farnaz Panahi; Amir Niasari-Naslaji; Fahimehsadat Seyedasgari; Tahereh Ararooti; Kamal Razavi; Ali Akbar Moosavi-Movaheddi
      Pages: 11 - 19
      Abstract: Publication date: September 2017
      Source:Animal Reproduction Science, Volume 184
      Author(s): Farnaz Panahi, Amir Niasari-Naslaji, Fahimehsadat Seyedasgari, Tahereh Ararooti, Kamal Razavi, Ali Akbar Moosavi-Movaheddi
      The present study was conducted to investigate a suitable source (Expt. 1) and concentration (Expt. 2) of plasma egg yolk (PEY) and concentration of camel skim milk (CSM; Expt. 3) to supplement tris based extender for chilled storage of dromedary camel semen. In Expt. 1, PEY (20%) of six avian species (domestic chicken, domestic duck, Japanese quail, partridge, pigeon and guinea fowl) was added to semen extender. In Expt. 2, different concentrations (0, 10, 20, 30 and 40%) of selected PEY from Expt.1 were added to semen extender. In both Expt. 1 and 2, CSM remained constant (20%). In Expt. 3, semen extender was supplemented with different concentrations of CSM (0, 20, 40, 60 and 80%) while the concentration of PEY remained constant. The sperm viability parameters were assessed at 6, 12 and 24h following chilled storage. In Expt. 1, progressive forward motility (PFM) of diluted semen supplemented with pigeon PEY was similar to domestic duck and Japanese quail PEYs (P>0.05) and superior to other PEYs (P<0.05). In Expt. 2, PFM following the addition of 20% pigeon PEY was similar to 10 and 30% (P>0.05) and greater than 0 and 40% (P<0.05). In Expt. 3, total motility, PFM and live percentage of sperm were better in 20% compared to 40, 60 and 80% CSM (P<0.05). In the last experiment, PFM in 20% was better than 0% CSM (P<0.05). In conclusion, pigeon PEY at the concentration of 20% and CSM at the concentration of 20% could provide beneficial effect on some of the sperm viability parameters during chilled storage of dromedary camel semen.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.06.008
      Issue No: Vol. 184 (2017)
  • Effect of liquid helium vitrification on cytoskeleton of immature cattle
    • Abstract: Publication date: Available online 16 October 2017
      Source:Animal Reproduction Science
      Author(s): Xian Fei Guo, Xue Li Yu, Fan Zhang, Hua Wu, Xu Zhe Pei, Xiao Xia Li, Ying Hua Li
      The developmental potential and the changes in cytoskeleton structures in immature oocytes of cattle resulting from liquid helium (LHe) vitrification was evaluated in this study. Immature oocytes were randomly divided into three groups: fresh oocytes (negative control), oocytes vitrified in liquid nitrogen (LN group, positive control), and oocytes vitrified in LHe (LHe group). In Experiment 1, the proportions for normal morphology, maturation, cleavage, and blastocyst were greater in the LHe group than in the LN group (88.3% compared with 79.1%, 51.7% compared with 43.3%, 42.6% compared with 33.0%, and 11.0% compared with 4.7%, respectively; P< 0.05), and the rates of oocyte development were greater in the control group (100%, 72.8%, 64.3%, and 40.3%) than in the vitrified groups (P <0.05). In Experiment 2, the effect of vitrification by LHe and LN on cytoskeleton of cattle oocytes was examined. The cytoskeleton had varying degrees of damage, and the negative influence of LHe vitrification on the cytoskeleton was less than that of LN vitrification (P <0.05), and the vitrified group had greater cytoskeleton degeneration than the control group (P <0.05). In conclusion, LHe vitrification reduced the negative effect of cryoinjury on cytoskeleton structure and improved the viability of immature oocytes of cattle compared with LN vitrification.

      PubDate: 2017-10-18T14:46:33Z
  • Comparison of DNA fragmentation of frozen-thawed epididymal sperm of dogs
           using Sperm Chromatin Structure Analysis and Sperm Chromatin Dispersion
    • Abstract: Publication date: Available online 12 October 2017
      Source:Animal Reproduction Science
      Author(s): I. Ortiz, M. Urbano, J. Dorado, J.M. Morrell, E. Al-Essawe, A. Johannisson, M. Hidalgo
      The aim of this study was to compare sperm DNA fragmentation of frozen-thawed epididymal sperm of dogs using the SCSA (Sperm Chromatin Structure Assay) and SCDt (Sperm Chromatin Dispersion test). For this purpose, epididymis from neutered dogs were minced and incubated in a Tris-based extender. The recovered sperm were frozen in a two-step cooling protocol with Tris-based, egg yolk extender and increasing glycerol concentrations, and stored in liquid nitrogen. After thawing, each replica was incubated at 38°C for 24h. Sperm DNA fragmentation index (sDFi) was assessed by SCSA and SCDt at 0, 3, 6 and 24h of incubation and compared within treatments. The relationship and agreement between techniques were evaluated by Pearson’s coefficient and Intraclass Correlation Coefficient (ICC). The results were expressed as mean±standard error of the mean (SEM). Both techniques indicated there was a significant increase of DNA fragmentation after 24h of incubation. Moderate correlation (r=0.65; P<0.01) but lack of agreement (ICC=0.451; P>0.05) was found between SCSA and SCDt. The lack of agreement indicates that SCSA and SCDt measure different aspects of DNA fragmentation. Four halo morphologies were detected after 24h of incubation using the SCDt: un-fragmented DNA with a small halo, fragmented DNA with large halo and two new halo presentations never described before for dog sperm: receding sperm with a disappearing halo and “bald” sperm without chromatin dispersion halo around the core. Sperm without a halo of chromatin dispersion are not described by the manufacturer and are similar to un-fragmented sperm, which could lead to erroneous results when using the SCDt. Further studies with different incubation periods and including the new morphologies described in this study should be performed. In conclusion, although SCSA and SCDt can evaluate the changes in the sperm DNA fragmentation dynamics of frozen-thawed epididymal dog sperm, these provided different findings on sperm DNA fragmentation.

      PubDate: 2017-10-18T14:46:33Z
  • PTGER2 activation induces PTGS-2 and growth factor gene expression in
           endometrial epithelial cells of cattle
    • Authors: Long Gao; Liu Wei Mao Ruifeng Gao Shuangyi Zhang Duritahala
      Abstract: Publication date: Available online 10 October 2017
      Source:Animal Reproduction Science
      Author(s): Long Gao, Bo Liu, Wei Mao, Ruifeng Gao, Shuangyi Zhang, Duritahala, Changqi Fu, Yuan Shen, Ying Zhang, Nan Zhang, Jindi Wu, Yang Deng, Xing Wu, Jinshan Cao
      The prostaglandin E2 receptor 2 (PTGER2) is present in the endometrium and its gene expression is accompanied with endometrial growth, however, it is unknown whether there is endometrial repair through stimulation of growth factor gene expression that is promoted by PTGER2 activation in cattle. The aim of this study was to investigate whether PTGER2 activation can induce prostaglandin-endoperoxide synthase-2 (PTGS-2) and growth factor gene expression by activating PKA and ERK signaling pathways in endometrial epithelial cells of cattle. Results demonstrated that the PTGER2 agonist, butaprost, induced cAMP/PKA and ERK activation and up-regulated PTGS-2, VEGF, CTGF, TGF-β1 and IL-8 gene expression. These activations were less after PTGER2 antagonist, AH6809, treatment. Data suggested that PTGS-2 gene expression was induced by PTGER2 activation through the PKA and ERK pathways. Furthermore, PTGER2 activation promoted several growth factor gene expressions in endometrial epithelial cells. One potential implication of this finding is that PTGER2 activation in the endometrium of cattle could induce endometrial repair by stimulating VEGF, CTGF, TGF-β1 and IL-8 gene expression.

      PubDate: 2017-10-11T20:05:08Z
  • Inhibition of deubiquitinases alters gamete ubiquitination states and
           sperm-oocyte binding ability in pigs
    • Authors: Yang Wang; Lili Zhuang Xuan Chen Man Zuochen Jin
      Abstract: Publication date: Available online 7 October 2017
      Source:Animal Reproduction Science
      Author(s): Yang Wang, Lili Zhuang, Xuan Chen, Man Xu, Zuochen Li, Yi Jin
      This study was undertaken to investigate the dynamics of protein ubiquitination in pig gametes and their micro-environments, as well as to explore the action of deubiquitinases (DUBs) in sperm-oocyte binding. Protein ubiquitination states were evaluated by in the ejaculated sperm, seminal plasma, epididymal sperm, oocytes, zona pellucida (ZP) and follicular fluid (FF) by western blotting. Different concentrations of PR-619, a non-selective inhibitor of DUBs, were used to treat oocytes during in vitro maturation (IVM), the maturation rate, amount of ubiquitinated ZP proteins, and ZP solubility were assessed. The PR-619 was also used to treat sperms during capacitation, then the ubiquitinated amounts of acrosin inhibitor (AI) proteins were evaluated. The number of sperm attached to the ZP of each oocyte was subsequently determined after gamete co-incubation. The study indicates the existence of ubiquitinated proteins (76kDa) in sperm, seminal plasma, oocytes, and follicular fluid (FF). The amount of ubiquitinated ZP proteins changed as growth of follicles progressed. Treatment with PR-619 at 10 and 15μM concentrations during IVM reduced the maturation rate of pig oocytes (P <0.05), while treatments with 10μM of PR-619 extended the ZP dissolution time (P <0.05). Treatment with PR-619 enhanced AI ubiquitination and improved amounts of 30-kDa ubiquitinated proteins (P <0.05). Treatment with PR-619 at the 10μM dose effectively reduced the number of sperm attached to per oocyte (P< 0.05). Ubiquitinated proteins were present in gametes and their micro-environments. The DUBs were important in regulating pig gamete ubiquitination and sperm-oocyte binding.

      PubDate: 2017-10-11T20:05:08Z
  • Oxytocin is not involved in luteolysis and early maternal recognition of
           pregnancy (MRP) in alpacas
    • Authors: Michela Ciccarelli; Muhammad Salman Waqas James Pru Ahmed Tibary
      Abstract: Publication date: Available online 6 October 2017
      Source:Animal Reproduction Science
      Author(s): Michela Ciccarelli, Muhammad Salman Waqas, James K. Pru, Ahmed Tibary
      Pregnancy maintenance depends on the maternal recognition of pregnancy (MRP), a physiological process by which the lifespan of the corpus luteum is prolonged. This mechanism is not well characterized in camelids. The objectives of the present research were to determine if exogenous oxytocin prolongs the corpus luteum activity in alpacas and to evaluate expression and localization of oxytocin receptors within the endometrium at 9 and 14days post-mating. In the oxytocin studies, plasma progesterone profiles were determined after ovulation in the same alpacas on 2 cycles: one cycle without oxytocin treatment and one cycle with oxytocin treatment. Oxytocin was administered daily by intramuscular injections (IM) at a dose of 20IU (experiment 1, n=6) or 60IU (experiment 2, n=7 from day 3 through day 10 after induction of ovulation with GnRH IM. There was no significant difference in the length of the luteal phase (i.e. corpus luteum lifespan) between the treated and control cycles using either 20 or 60IU of oxytocin. In the final experiment, uteri from open and pregnant alpacas (n=4 per group) at 9 and 14days post-mating were evaluated for expressions of oxytocin receptors by immunohistochemistry. No significant difference (P≤0.05) in the expression of oxytocin receptors was observed between open and pregnant animals in either staining intensity or tissue localization. We conclude that oxytocin is not involved in luteolysis and early MRP in alpacas.

      PubDate: 2017-10-11T20:05:08Z
  • Sperm subpopulations in ejaculated sperm and spermatozoa recovered from
           ovine epididymides up to 48h after death
    • Authors: Tácia Gomes Bergstein-Galan; Romildo Romualdo Weiss; Luiz Ernandes Kozicki; Sony Dimas Bicudo
      Abstract: Publication date: Available online 5 October 2017
      Source:Animal Reproduction Science
      Author(s): Tácia Gomes Bergstein-Galan, Romildo Romualdo Weiss, Luiz Ernandes Kozicki, Sony Dimas Bicudo
      The objectives of this study were threefold: to identify subpopulations of sperm based on the kinetics of frozen/thawed sheep epididymal spermatozoa or semen collected with an artificial vagina; to evaluate the effects on sperm subpopulations in the thawed samples of post mortem storage at room temperature and the addition of 20% of seminal plasma to the freezing extender and to correlate the percentage of subpopulations with gestation rate following artificial intrauterine insemination. The categorization of the subpopulations was based on sperm kinetic data from Computer Assisted Sperm Analysis (CASA). A hundred ewes were inseminated with thawed spermatozoa and gestation rate was correlated with the proportions of each subpopulation using Pearson correlation matrix and linear regression. Three distinct subpopulations were identified in the thawed samples of either ovine ejaculate collected in artificial vaginas (AV) or ovine spermatozoa retrieved from the cauda epididymis. Subpopulation 1 (SP1) was characterized by spermatozoa with slow and non-linear motion, subpopulation 2 (SP2) was classified as hyperactived spermatozoa and subpopulation 3 (SP3) was composed of spermatozoa with fast, linear motion. The largest subpopulation in all groups was SP1. The semen collected in an artificial vagina had a higher (P<0.05) percentage of SP2 and lower (P<0.05) percentage of SP1 when compared to spermatozoa recovered after death. Increasing time of storage after death had a detrimental effect on sperm samples, increasing (P<0.05) the percentage of SP1 and decreasing (P<0.05) SP2. Length of storage after death was the only variable that influenced, with an inversely proportional relationship, SP3. In samples stored for 48h after death no SP3 spermatozoa were present. The addition of seminal plasma to the cryopreservative decreased (P<0.05) the subpopulation of hyperactived spermatozoa (SP2). We conclude that, after thawing there are three sperm subpopulations in the spermatozoa obtained from the cauda epididymides and the semen collected in AVs and that the relative proportions of these subpopulations varies with the time of storage post mortem and the presence of 20% of seminal plasma in the extender. However, we conclude that these subpopulations do not correlate with fertility after intrauterine artificial insemination.

      PubDate: 2017-10-11T20:05:08Z
      DOI: 10.1016/j.anireprosci.2017.10.001
  • Effect of season on follicular population, quality and nuclear maturation
           of bovine oocytes under tropical conditions
    • Authors: Jorge Alonso Peralta-Torres; Jesús Ricardo Aké-López; José Candelario Segura-Correa; Jesús Ricardo Aké-Villanueva
      Abstract: Publication date: Available online 5 October 2017
      Source:Animal Reproduction Science
      Author(s): Jorge Alonso Peralta-Torres, Jesús Ricardo Aké-López, José Candelario Segura-Correa, Jesús Ricardo Aké-Villanueva
      The aim was to determine the effect of season of the year and the presence of a corpus luteum (CL) on follicular population (FP) and the quality of the oocytes, and of season on nuclear maturation of the bovine oocytes under tropical conditions. Three seasons were evaluated: hot-dry (March–June), hot-humid (July–October) and fresh-humid (November–February). In a first study, 1112 bovine ovaries were obtained from a local slaughterhouse. Follicles were classified as small (≤4mm), middle (4.1–8mm) and large (≥8.1mm); and the maximum diameter of the follicle (MDF) and CL (MDCL) were also recorded. The oocytes were collected by aspiration and classified as viable (grade I and II) and damaged (grade III and IV). In the second study, 2261 viable oocytes were matured in vitro, and then fixed and stained with Lacmoid to classify the stage of development as mature (metaphase II), immature or degenerate. Data were analyzed using analysis of variance and chi-square procedures. The largest FP of large follicles (0.67), MDF (1.18mm), MDCL (1.87mm), and the highest proportion of viable oocytes (34.19%) were obtained during the hot-humid season (P<0.05). The ovaries without CL had the greatest FP (10.34) with more viable oocytes (24.44%). The highest proportion of mature oocytes (76.92%) was also obtained in the hot-humid season. In conclusion, season influenced FP, MDF, MDCL, and the quality and nuclear maturation of oocytes. The presence of a CL in the ovary resulted in a decrease of FP and viability of oocytes.

      PubDate: 2017-10-11T20:05:08Z
      DOI: 10.1016/j.anireprosci.2017.10.004
  • Prediction of calving time in dairy cattle
    • Authors: Fadul Mahmoud; Bogdahn Christopher; Alsaaod Maher; Hüsler Jürg; Starke Alexander; Steiner Adrian; Hirsbrunner Gaby
      Abstract: Publication date: Available online 5 October 2017
      Source:Animal Reproduction Science
      Author(s): Fadul Mahmoud, Bogdahn Christopher, Alsaaod Maher, Hüsler Jürg, Starke Alexander, Steiner Adrian, Hirsbrunner Gaby
      This prospective study was carried out to predict the calving time in primiparous (n=11) and multiparous (n=22) Holstein-Friesian cows using the combination of data obtained from the RumiWatch noseband-sensor and 3D-accelerometer. The animals included in the study were fitted with the RumiWatch noseband-sensor and 3D-accelerometer at least 10days before the expected calving day. The calving event was defined as the time of the first appearance of the calves’ feet outside the vulva, and this moment was determined by farm staff and/or confirmed by video monitor. As primiparous and multiparous cows behaved differently, two models including data of noseband-sensors and 3D-accelerometers were used to predict the calving time in each group. Lying bouts (LB) increased and rumination chews (RC) decreased similarly in both groups; besides that, boluses (B) decreased and other activities (OA) increased significantly in multiparous and primiparous cows, respectively. The sensitivity (Se) and specificity (Sp) for prediction of the onset of calving within the next 3h were determined with the logistic regression and ROC analysis (Se=88.9%, 85% and Sp=93.3%, 74% for multiparous and primiparous cows, respectively). This pilot study revealed that the RumiWatch system is a useful tool to predict calving time under farm conditions.

      PubDate: 2017-10-11T20:05:08Z
      DOI: 10.1016/j.anireprosci.2017.10.003
  • HSP90 maintains boar spermatozoa motility and mitochondrial membrane
           potential during heat stress
    • Authors: V. Calle-Guisado; M.J. Bragado; L.J. García-Marín; L. González-Fernández
      Abstract: Publication date: Available online 29 September 2017
      Source:Animal Reproduction Science
      Author(s): V. Calle-Guisado, M.J. Bragado, L.J. García-Marín, L. González-Fernández
      Heat Shock Proteins (HSP) is a family of proteins that protects cells from high temperatures. The present work aimed to elucidate the role that HSP90 exerts on boar sperm incubated under heat stress conditions on viability, total motility (TM), progressive motility (PM), acrosome status, mitochondrial membrane potential and plasma membrane lipid organization. Sperm were incubated in non-capacitating conditions (Tyrode’s basal medium or TBM) for 3, 8 and 24h or in capacitating conditions (Tyrode’s complete medium or TCM) for 4h at 38.5°C or 40°C (Heat stress) in the presence or absence of 5 or 20μM of 17-AAG, a specific HSP90 inhibitor. Sperm viability was not affected by the presence of 17-AAG in any condition tested compared with its own control (at same the temperature and incubation time). In non-capacitating conditions TM (22.7±4.1 vs. 1.9±1.1; % mean±SEM), PM (3.1±0.9 vs. 0) and high mitochondrial membrane potential (19.5±2.2 vs. 11.8±0.8) decreased significantly in sperm incubated at 40°C for 24h in the presence of 20μM 17-AAG (control vs. 20μM 17-AAG, respectively; p<0.05). In sperm incubated at 38.5°C only a mild decrease in TM was observed (48.7±3.1 vs. 32.1±4.8; control vs. 20μM 17-AAG, respectively; p<0.05). However, under capacitating conditions none of the sperm parameters studied were affected by 17-AAG after 4h of incubation. These results demonstrate for first time the role of HSP90 in the maintenance of boar sperm motility and mitochondrial membrane potential during prolonged heat stress in non-capacitating conditions.

      PubDate: 2017-10-03T19:25:10Z
      DOI: 10.1016/j.anireprosci.2017.09.009
  • Fetal sex alters maternal anti-Mullerian hormone during pregnancy in
    • Authors: Anja Stojsin-Carter; Nathalia N. Costa; Rodrigo De Morais; Tiago H. De Bem; Mayra P. Costa; Timothy F. Carter; Daniel J. Gillis; Michael S. Neal; Otavio M. Ohashi; Moyses S. Miranda; Flavio V. Meirelles; Laura A. Favetta; W. Allan King
      Abstract: Publication date: Available online 22 September 2017
      Source:Animal Reproduction Science
      Author(s): Anja Stojsin-Carter, Nathalia N. Costa, Rodrigo De Morais, Tiago H. De Bem, Mayra P. Costa, Timothy F. Carter, Daniel J. Gillis, Michael S. Neal, Otavio M. Ohashi, Moyses S. Miranda, Flavio V. Meirelles, Laura A. Favetta, W. Allan King
      Anti-Mullerian hormone (AMH) is expressed by both male and female fetuses during mammalian development, with males expressing AMH earlier and at significantly higher concentration. The aim of the current study was to explore the potential impact of pregnancy and fetal sex on maternal AMH and to determine if plasma (Pl) AMH or placenta intercotyledonary membrane and cotyledonary AMH receptor 2 (AMHR2) mRNA expression differ in pregnant cows carrying male vs. female fetuses. AMH levels in blood were measured using a bovine optimized ELISA kit. Cows pregnant with a male fetus were observed to have a significantly greater difference in Pl AMH between day 35 and 135 of gestation. Average fetal AMH level between 54 and 220days of gestation was also observed to be significantly higher in male vs. female fetuses. Intercotyledonary membranes and cotyledons were found to express AMHR2 between days 38 and 80 of gestation at similar levels in both fetal sexes. These findings support the hypothesis that fetal sex alters maternal Pl AMH during pregnancy in cattle.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.09.010
  • Current status of the role of endothelins in regulating ovarian follicular
           function: a review
    • Authors: J.M. Ervin; L.F. Schütz; L.J. Spicer
      Abstract: Publication date: Available online 21 September 2017
      Source:Animal Reproduction Science
      Author(s): J.M. Ervin, L.F. Schütz, L.J. Spicer
      Endothelins (EDN) are a group of vasoactive 21 amino acid peptides reported to play roles in steroidogenesis, folliculogenesis, and ovulation. EDN1, EDN2 and EDN3 have all been shown to affect granulosa cell (GC) function in a variety of mammalians species. Herewithin, the role of EDN in regulating steroidogenesis and ovarian follicular development is reviewed, focusing on the localization and function of EDN and their receptors in ovarian follicular function emphasizing species differences. For example, in single ovulating species such as humans and cattle, in the presence of trophic hormones such as FSH and IGF1, EDN1 and EDN2 significantly inhibited GC estradiol production in 2 of 4 studies, while no effect was observed for GC progesterone production in 2 of 4 studies. In contrast, EDN1 exhibited inhibitory effects on progesterone production by GC in 3 of 3 studies in pigs and 3 of 4 studies in rats. Also, EDN1 inhibited GC estradiol production in 4 of 5 studies in rats. Altogether, these results indicate that EDN are produced by ovarian follicles and are involved in the regulation of steroidogenesis of GC of several mammalian species including humans, cattle, pigs and rats, but that these effects may vary with species and culture condition.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.09.008
  • Pregnancy-associated changes in expression of progesterone receptor and
           progesterone-induced blocking factor genes in bone marrow of ewes
    • Authors: Le-Ying Zhang; Hao Mi; Jin-Kun Yan; Xian-Xi Yan; Ling Yang
      Abstract: Publication date: Available online 21 September 2017
      Source:Animal Reproduction Science
      Author(s): Le-Ying Zhang, Hao Mi, Jin-Kun Yan, Xian-Xi Yan, Ling Yang
      Progesterone (P4) regulates reproductive and immune functions through binding to the progesterone receptor (PGR), and the effects of P4 are partly mediated by a progesterone-induced blocking factor (PIBF). Bone marrow (BM) is a key component of the lymphatic system and has an important role in immune response. In this study, BM was harvested from femurs on days 13, 16 and 25 of pregnancy and day 16 of the estrous cycles without mated by intact rams, and a qRT-PCR assay, Western blot and an immunohistochemistry analysis were used to analyze the expression of PGR and PIBF genes in BM. The results showed that there was an increase in relative abundance of PGR and PIBF mRNA in BM during early pregnancy, and PGR-B and the full-length PIBF genes were up-regulated in pregnant ewes. Immunohistochemistry results confirmed that the PGR and PIBF proteins were localized in both the cytoplasm and nuclei of adipocytes and the cells in the stroma and capillaries. This is the first study reporting an up-regulated expression of PGR-B and full-length PIBF genes in BM during early pregnancy in sheep. It is suggested that the conceptus exerted its effects on the adipocytes and the cells in the stroma and capillaries in BM, which were involved in the immunoregulation of BM through both cytosolic and nuclear pathways in ewes.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.09.007
  • Improvement of liquid stored boar semen quality by removing low molecular
           weight proteins and supplementation with α-tocopherol
    • Authors: M. Zakošek Pipan; J. Mrkun; A. Nemec Svete; P. Zrimšek
      Abstract: Publication date: Available online 20 September 2017
      Source:Animal Reproduction Science
      Author(s): M. Zakošek Pipan, J. Mrkun, A. Nemec Svete, P. Zrimšek
      Seminal plasma contains low-molecular weight components that can exert a harmful effect on sperm function. We have evaluated the effects of removing low-molecular weight components from seminal plasma and adding α-tocopherol on boar semen quality after 72h of liquid storage. Semen was evaluated on the basis of motility, morphology, acrosome integrity, plasma membrane modifications, mitochondrial activity, DNA fragmentation and lipid peroxidation. Thiobarbituric acid reactive substances (TBARS), 8-isoprostane, and antioxidant status (total antioxidant capacity (TAC) and superoxide dismutase activity (SOD)) were measured in seminal plasma. Removal of low-molecular weight components from seminal plasma, together with the addition of α-tocopherol, kept the lipid peroxidation and mitochondrial activity and DNA fragmentation at the same level as in native semen samples. Dialysing semen and adding 200μM of α-tocopherol led to higher progressive motility, a higher proportion of morphologically normal spermatozoa and a significantly lower level of acrosomal reacted spermatozoa compared to non-dialyzed semen samples after 72h of storage. In conclusion, liquid stored boar semen was better preserved, and oxidative stress in the semen was reduced when semen was dialyzed and α-tocopherol was added prior to storage.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.09.004
  • Beneficial effects of dietary soluble fiber supplementation in replacement
           gilts: Pubertal onset and subsequent performance
    • Authors: Yong Zhuo; Xiaolin Shi; Gang Lv; Lun Hua; Pan Zhou; Liangqiang Che; Zhengfeng Fang; Yan Lin; Shengyu Xu; Jian Li; Bin Feng; De Wu
      Abstract: Publication date: Available online 17 September 2017
      Source:Animal Reproduction Science
      Author(s): Yong Zhuo, Xiaolin Shi, Gang Lv, Lun Hua, Pan Zhou, Liangqiang Che, Zhengfeng Fang, Yan Lin, Shengyu Xu, Jian Li, Bin Feng, De Wu
      The aim of this study was to examine the effects of soluble fiber supplementation prior to puberty on age at puberty and subsequent reproductive performance of gilts. A total of 136 gilts of similar body weight (BW, 60.59±7.02kg) and age (140±10 days) were fed a control diet (CON) or control diet supplemented with 0.8% soluble fiber (SF) until mating at the third estrus. Circulating concentrations of cholesterol, triglyceride, and estradiol in gilts fed the SF diet were lower than in CON gilts at 205d of age. Compared with CON-fed gilts, the SF-fed gilts attained observed puberty 15.6d earlier (P< 0.05), at a 12.2kg lower body weight, and a 0.84mm lower backfat thickness at the P2 point (P <0.05). The total number of piglets born, the number born alive, and average birthweight, were not affected by diet (P >0.05). However, the incidence of intrauterine growth restriction (IUGR) was lower for SF gilts (4.62%) than for CON gilts (11.3%) (P< 0.05). There was also a greater intra-litter uniformity (P< 0.05) and a tendency for a higher number of piglets born in the SF gilts compared with the CON gilts (P =0.07). In summary, prepubescent dietary soluble fiber supplementation can reduce the age at puberty in gilts and increase their subsequent reproductive performance as sows.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.08.007
  • High energetic cost of oviposition in an edible marine gastropod
    • Authors: Andrés Averbuj; Daniel Fernández; Pablo E. Penchaszadeh; Gregorio Bigatti
      Abstract: Publication date: Available online 17 September 2017
      Source:Animal Reproduction Science
      Author(s): Andrés Averbuj, Daniel Fernández, Pablo E. Penchaszadeh, Gregorio Bigatti
      The edible neogastropod Buccinanops cochlidium from Patagonia, Argentina, reproduces by means of egg capsules attached by the female to its own shell. Gravid females lay an outstanding mean of 500,000 eggs that nurse around 800 embryos, which hatch as crawling juveniles (4mm in shell length) after four months of intracapsular development. This reproductive investment could be expressed as the energy content (EC) estimated for the production of a complete egg mass (33.94±12.85 KJ), representing a conservative estimation of the total EC needed for an adult female to spawn, which is in average ∼12% of the total EC in gravid females. This high maternal investment allows a considerable offspring size, which confers them high survival chances. A translocation of energy stored in the foot during the oviposition season is shown in a relative decrease of ∼10% in the foot EC in respect of the total EC (61.8% in non-gravid females vs. 51.3% in gravid females). Gravid females showed significantly higher body wet mass/shell length index and higher total EC than non-gravid females (266.0±66.4 KJ vs. 184.3±69.6 KJ), suggesting that a body condition threshold is required for females to reproduce. These values represent an energetic surplus of over 40% of the total EC per individual when compared to non-gravid females. Protecting gravid females from fisheries would ensure the sustainability of the resource and must be taken into account when establishing fisheries policies.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.09.005
  • Luteolytic efficiency of reduced doses of cloprostenol in the ewe. Effect
           of progesterone concentrations at the time of treatment
    • Authors: Luz María Granados-Villarreal; Luis Zarco; Octavio Mejía; María Teresa Sánchez-Torres; José Luis Pablos-Hach
      Abstract: Publication date: Available online 17 September 2017
      Source:Animal Reproduction Science
      Author(s): Luz María Granados-Villarreal, Luis Zarco, Octavio Mejía, María Teresa Sánchez-Torres, José Luis Pablos-Hach
      Seventy six ewes were treated with 7.5, 12.5, 25 or 50μg of cloprostenol on day 6 or 9 post-estrus to compare the luteolytic efficiency of the PGF2α analogue at each stage and to evaluate if progesterone concentrations at the time of treatment affect such efficiency. Blood samples were obtained before cloprostenol administration and 12, 24, 48, and 72h thereafter. There was an effect of dose (p<0.05) but not of day post-estrus on the proportion of animals completing luteolysis. As the dose increased, the proportion of ewes completing luteolysis also increased. Also, as the dose increased from 7.5 to 25μg, more ewes showed a transient progesterone decline instead of an absence of response, indicating that in some ewes reduced doses initiated luteolysis but were not able to finish the process. Since the dose of 25μg resulted in close to 50% luteolytic efficacy, this group was used to study the effects of progesterone concentrations at the time of treatment on the response to cloprostenol. Pre-treatment progesterone concentrations were higher (p<0.01) in ewes experiencing luteolytic failure than in those that completed luteolysis. There was a negative correlation between initial progesterone concentrations and their reduction by 12h post-treatment. It is concluded that high progesterone concentrations are associated with a reduction in sensitivity to small doses of cloprostenol. Possible mechanisms and implications of this luteoprotective effect are discussed.

      PubDate: 2017-09-26T05:58:05Z
      DOI: 10.1016/j.anireprosci.2017.09.006
  • Pre-ovulatory follicle affects corpus luteum diameter, blood flow, and
           progesterone production in mares
    • Authors: G.M. Ishak; S.T. Bashir; M.O. Gastal; E.L. Gastal
      Abstract: Publication date: Available online 8 September 2017
      Source:Animal Reproduction Science
      Author(s): G.M. Ishak, S.T. Bashir, M.O. Gastal, E.L. Gastal
      Color Doppler ultrasonography was used to study the temporal relationships between pre-ovulatory follicle (POF) and corpus luteum (CL) diameter and blood flow, with systemic progesterone (P4) concentration during two transitional ovulatory seasons in mares. Variables of POF and CL/P4 were evaluated for 6days before and 17days after ovulation, respectively. Evaluations were performed during two consecutive estrous cycles in spring and fall seasons, and during the last estrous cycle of the season. There were significant correlations among POF and CL variables, and P4 concentration that ranged from 0.24 to 0.95, and among the ratios of different variables that ranged from 0.39 to 0.92. There were linear regressions (P< 0.01−0.001) for all comparisons among different variables. The POF diameter before the first ovulation of the season was larger (P< 0.05), and POF vascularity was less (P< 0.05), than in the last estrous cycle during the season. The CL blood flow was less (P ˂ 0.01) during the last compared with first pre-ovulatory period of the season. The POF diameters were positively correlated (r =0.67) during the two pre-ovulatory periods of spring and fall. Results provide evidence that the POF affects CL diameter and blood flow, and subsequently P4 production, and that POF diameter is repeatable within the same individual during different seasons.

      PubDate: 2017-09-13T20:31:23Z
      DOI: 10.1016/j.anireprosci.2017.09.003
  • Nanos2 is a molecular marker of inchoate buffalo spermatogonia
    • Authors: Meng-Qi Li; Ao-Lin Luo; Peng-Wei Zhao; Ting-Ting Li; Shuang-Shuang Geng; Xing-Wei Liang; Hui-Yan Xu; Yang-Qing Lu; Sheng-Sheng Lu; Xiao-Gan Yang; Ke-Huan Lu
      Abstract: Publication date: Available online 8 September 2017
      Source:Animal Reproduction Science
      Author(s): Meng-Qi Li, Ao-Lin Luo, Peng-Wei Zhao, Ting-Ting Li, Shuang-Shuang Geng, Xing-Wei Liang, Hui-Yan Xu, Yang-Qing Lu, Sheng-Sheng Lu, Xiao-Gan Yang, Ke-Huan Lu
      Nanos2 belongs to the Nanos gene-coding family and is an important RNA-binding protein that has been shown to have essential roles in male germline stem cells development and self-renewal in mouse. However, little is known about Nanos2 in inchoate buffalo spermatogonia. Here, rapid-amplification of cDNA ends (RACE) was used to obtain the full-length buffalo Nanos2 sequence and bioinformatic analysis revealed a highly conserved Nanos2 sequence between buffalo and other mammalian species. Although Nanos2 was expressed in various tissues, the highest mRNA expression levels were found in testes tissue. Moreover, Nanos2 mRNA was abundant in fetal and pre-puberal testes but markedly decreased in the testes of adults. At the protein level, immunohistochemistry in pre-puberal testes revealed a pattern of NANOS2 expression similar to that for the undifferentiated type A spermatogonia marker PGP9.5. Furthermore, NANOS2 expression was low in adult testes and restricted to elongating spermatids. Altogether, our data suggest that Nanos2 is a potential preliminary molecular marker of inchoate buffalo spermatogonia, and may play an important role in buffalo spermatogonial stem cells (SSCs) development and self-renewal, as has been observed in other model animals.

      PubDate: 2017-09-13T20:31:23Z
      DOI: 10.1016/j.anireprosci.2017.09.002
  • Effects of GnRHR polymorphisms on sperm quality in Chinese water buffalo
    • Authors: Gang Wang; Linlin Hao; Yunyun Cheng; Shuai Li; Yu Zhang; Chen Lv; Wenzhen Wei; Shan Huang; Hongyu Shi; Lijie Dong; Yifan Zhang; Hao Yu; Jiabao Zhang
      Abstract: Publication date: Available online 5 September 2017
      Source:Animal Reproduction Science
      Author(s): Gang Wang, Linlin Hao, Yunyun Cheng, Shuai Li, Yu Zhang, Chen Lv, Wenzhen Wei, Shan Huang, Hongyu Shi, Lijie Dong, Yifan Zhang, Hao Yu, Jiabao Zhang
      Gonadotropin-releasing hormone receptor (GnRHR) plays a critical physiological role in animal reproduction and is a potential marker for improving sperm quality. In the present study, eight SNPs (g.539T>C, g.640A>G, g.655T>C, g.707T>C, g.812A>G, g.18951A>T g.16867T>C and g.18953Indel GGCAAAGTAA) were detected in the GnRHR gene from one-hundred-sixty-five water buffalo by direct sequencing and identification of overlapping peaks. All SNPs were associated significantly with the ejaculate volume and two genes (g.655T>C and g.707T>C) were correlated with sperm abnormalities. Furthermore, three haplotypes (H1:TAI, H2:CT-, and H3:TT-) were identified by linkage disequilibrium analysis and were composed of four combined genotypes. Notably, buffalo with the combined genotypes H1H2 and H1H3 had the higher ejaculate volume compared to the other combined genotypes. Among the eight SNPs and four combined genotypes, the deletion of GGCAAAGTAA at position 18953bp in GnRHR was associated significantly with a higher ejaculate volume. Moreover, the GGCAAAGTAA deletion may lead to the miR8661 binding failure and subsequent changes in GnRHR gene expression. In the present study, we demonstrate that there is a significant association between SNPs in the GnRHR gene and the sperm ejaculate volume of Chinese water buffalo. To the best of our knowledge, this study is the first to address the association between the SNPs in the GnRHR gene and the sperm quality of Chinese buffalo.

      PubDate: 2017-09-08T05:16:24Z
      DOI: 10.1016/j.anireprosci.2017.09.001
  • Resumption of ovarian function, the metabolic profile and body condition
           in Brahman cows (Bos indicus) is not affected by the combination of calf
           separation and progestogen treatment
    • Authors: Ramiro Díaz; Carlos S. Galina; Ivette Rubio; Manuel Corro; José Luis Pablos; Ana Rodríguez; Agustín Orihuela
      Abstract: Publication date: Available online 1 September 2017
      Source:Animal Reproduction Science
      Author(s): Ramiro Díaz, Carlos S. Galina, Ivette Rubio, Manuel Corro, José Luis Pablos, Ana Rodríguez, Agustín Orihuela
      To evaluate the effect of different calf separation procedures after a progestogen treatment on the resumption of ovarian function, body condition and metabolic profile, 59 multiparous Brahman cows grazing on a mixed grass pasture were studied. No supplementation was given at any time. Body condition score (BCS), fat thickness (FAT) and blood metabolites were measured fortnightly from the beginning of the last trimester of gestation until 96days postpartum. At 30days postpartum all animals received a progesterone (P4)-releasing device (CIDR) which was withdrawn 9days later when prostaglandin F2α was applied. At this time, treatments TW (n =28), where calves were separated from their dams for 48h; RS (n =21), calves were allowed to suckle once a day for 1h.; and continuous suckling (CS; n =10). Ovarian function was assessed by blood concentrations of progesterone on days −14, −9, 10, 13, 30 and 33 after CIDR removal. At the end of the experimental period, an average of 20% of the cows had not initiated estrous cycles. There were no changes of FAT or BCS during the last trimester of pregnancy in all cows (P> 0.05). During the postpartum period cows of all groups lost (P <0.05) BCS and FAT with a nadir at 60 to 80days postpartum, regardless of treatment. At 10days after CIDR withdrawal the percentage of cows having ovulations was 75, 61 and 80 (P >0.05) for TW, RS and CS groups. Blood metabolites follow a similar pattern in the three groups. With the conditions of the present study, the method of calf separation after a progestogen treatment, does not affect the resumption of ovarian function or metabolic profile.

      PubDate: 2017-09-08T05:16:24Z
      DOI: 10.1016/j.anireprosci.2017.08.018
  • Cryopreservation of sperm in Grey mullet Mugil cephalus (Linnaeus, 1758)
    • Authors: Ramachandran Balamurugan; Natesan Munuswamy
      Abstract: Publication date: Available online 30 August 2017
      Source:Animal Reproduction Science
      Author(s): Ramachandran Balamurugan, Natesan Munuswamy
      The aim of this study was to document the effects of cryopreservation on sperm motility and viability in Grey mullet Mugil cephalus. Cryopreservation of sperm was attempted by using two extenders ringer solution for marine fish (RSMF) and V2 extender (V2E) and cryoprotectants dimethylacetamide (DMA), dimethylsulfoxide (DMSO), ethylene glycol (EG), glycerol (GLY), propylene glycol (PG) and methanol (MeOH). Cryoprotectants were assessed at different concentrations individually as well as in combination with varying equilibration times (10 and 30min). For optimization of freezing rate, four freezing protocols (−5, −10, −20 and −30°C/min) were evaluated. After achieving final temperature, samples were plunged in liquid nitrogen (−196°C) and stored for a week. Samples were subsequently thawed in a water bath at 30°C for assessment of sperm motility and viability. Results indicated that cryomedium constituting of V2E extender+10% glycerol with a dilution ratio of 1:1 (sperm: cryomedium) at an equilibration time of 5 to- 10min and freezing rate of −20°C/min was more desirable compared with other factors that were assessed. Use of this protocol resulted in retaining the greatest sperm motility grade 3.0±0.0 (50%–80% sperm movement, fast swimming) and 48.19±3.12% of sperm viability. The results of the present study, therefore, provide base-line data for establishing a protocol for sperm cryopreservation in M.cephalus. Further studies are, however, required for optimization of most suitable sperm cryopreservation protocol.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.022
  • Enhancement of sperm motility and viability by turmeric by-product dietary
           supplementation in roosters
    • Authors: Wenjing Yan; Chihiro Kanno; Eiki Oshima; Yukiko Kuzuma; Sung-Woo Kim; Hanako Bai; Masashi Takahashi; Yojiro Yanagawa; Masashi Nagano; Jun-ichi Wakamatsu; Manabu Kawahara
      Abstract: Publication date: Available online 30 August 2017
      Source:Animal Reproduction Science
      Author(s): Wenjing Yan, Chihiro Kanno, Eiki Oshima, Yukiko Kuzuma, Sung-Woo Kim, Hanako Bai, Masashi Takahashi, Yojiro Yanagawa, Masashi Nagano, Jun-ichi Wakamatsu, Manabu Kawahara
      Improving sperm motility and viability are major goals to improve efficiency in the poultry industry. In this study, the effects of supplemental dietary turmeric by-product (TBP) from commercial turmeric production on sperm motility, viability, and antioxidative status were examined in domestic fowl. Mature Rhode Island Red roosters were divided into two groups – controls (groupC) without TBP administration and test subjects (groupT) fed a basal diet supplemented with 0.8g of TBP/day in a temperature-controlled rearing facility (Experiment 1) and 1.6g/day under heat stress (Experiment 2) for 4 weeks. In Experiment 1, TBP dietary supplementation increased the sperm motility variables straight-line velocity, curvilinear velocity, and linearity based on a computer-assisted semen analysis, 2 weeks following TBP supplementation. In Experiment 2, using flow cytometry, sperm viability at 3 and 4 weeks following TBP supplementation was greater in Group T than C, and this increase was consistent with a reduction in reactive oxygen species (ROS) production at 2 and 4 weeks. The results of both experiments clearly demonstrate that dietary supplementation with TBP enhanced sperm motility in the controlled-temperature conditions as well as sperm viability, and reduced ROS generation when heat stress prevailed. Considering its potential application in a range of environments, TBP may serve as an economical and potent antioxidant to improve rooster fertility.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.021
  • Anandamide exerts a suppressive effect on sperm binding to oviduct
           explants through CB1 receptors in the water buffalo (Bubalus bubalis)
    • Authors: Vimlesh Kumar; Arumugam Kumaresan; Puneeth Kumar D.S; Sreela Lathika; Samiksha Nayak; Kaustubh Kishor Saraf; Pradeep Nag B.S; Shivani Chhillar; Tirtha Kumar Datta; Tushar Kumar Mohanty
      Abstract: Publication date: Available online 26 August 2017
      Source:Animal Reproduction Science
      Author(s): Vimlesh Kumar, Arumugam Kumaresan, Puneeth Kumar D.S, Sreela Lathika, Samiksha Nayak, Kaustubh Kishor Saraf, Pradeep Nag B.S, Shivani Chhillar, Tirtha Kumar Datta, Tushar Kumar Mohanty
      An endocannabinoid system comprising of Anandamide (AEA) and its receptor has been shown to play a role in sperm acquisition of fertilizing potential and sperm-oviduct interaction. In the present study, we assessed the effect of sperm pre-treatment with AEA or co-incubation of sperm-oviduct explants with AEA in the presence or absence of CB1 receptor antagonist (SR141716A) on sperm-oviduct binding in the water buffalo. Cryopreserved spermatozoa from 3 Murrah buffalo bulls (3 ejaculates from each bull) were utilized for the study. Oviduct explants were prepared by overnight culture of epithelial cells in TCM- 199 and washed spermatozoa were added to the oviduct explants and incubated for 1h. Then, sperm-oviduct explants were stained with a fluorescent stain (JC-1) and sperm binding index (BI – No. of bound spermatozoa/unit area of oviduct explants) was assessed. The results indicate that BI decreased significantly (P<0.05) when spermatozoa were either pre-treated with AEA (14.16±0.87) or sperm-oviduct explants were co-incubated with AEA (16.27±0.86) at 1nM concentration compared to the control group (29.12±2.17), however such effect was not observed when AEA was used at 1μM concentration. Incorporation of SR141716A in the incubation medium inhibited the suppressive effect of AEA on BI. It was concluded that AEA, at 1nM concentration, decreased the number of spermatozoa bound to the oviduct explants and the suppressive effect of AEA on sperm-oviduct binding was inhibited by CB1 receptor antagonist suggesting that the effect of AEA was mediated through CB1 receptor in the water buffalo.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.020
  • Gonadorelin increases semen production and does not affect its quality in
           Leporinus obtusidens
    • Authors: Jurandir Joaquim Bernardes Júnior; Robie Allan Bombardelli; Alex Pires de Oliveira Nuñer
      Abstract: Publication date: Available online 24 August 2017
      Source:Animal Reproduction Science
      Author(s): Jurandir Joaquim Bernardes Júnior, Robie Allan Bombardelli, Alex Pires de Oliveira Nuñer
      Use of carp pituitary extract (CPE) as a hormone inducer for Leporinus obtusidens is cost-prohibitive; moreover, CPE contains unknown concentrations of gonadotropins. We evaluated the efficacy of gonadorelin as a substitute by analyzing its effect on sperm characteristics of L. obtusidens and cost-effectiveness (20, 40, 60, 80, and 100μgkg−1). Untreated and CPE-treated (4mgkg−1) fish were used as negative and positive controls, respectively. Following a single intramuscular dose of hormones, semen was collected at 200°-h. At doses from 60μgkg−1, all fish produced higher milt volume (P <0.05) than the negative control (0.2–0.8mLkg−1), equal (P >0.05) to the positive control (3.52±0.97mLkg−1). Gonadorelin did not affect sperm concentration (P> 0.05). The percentage of morphologically normal spermatozoa was >64% in all treatments. The number of spermatozoa with intact membrane (70%–85%) was equal (P >0.05) to that in the controls. Relative to the negative control, gonadorelin did not affect the motility rate (P >0.05). Curvilinear velocity was equal (P >0.05) to that in both controls. The sperm swimming path was linear (0.9) 10s after activation and was not (P >0.05) dose-dependent. Considering the response rate of gonadorelin-induced fish and the similarity of semen quality compared to controls, the lowest effective dose to produce a higher volume of semen in L. obtusidens is 60μgkg−1, and the cost to induce 1kg of L. obtusidens is 14.0% lower than that with CPE.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.015
  • Analysis of putative biomarkers of undifferentiated spermatogonia in dog
    • Authors: Won-Young Lee; Hyun-Jung Park; Ran Lee; Ji-Heon Lee; Hyunjhung Jhun; Tai-Young Hur; Hyuk Song
      Abstract: Publication date: Available online 24 August 2017
      Source:Animal Reproduction Science
      Author(s): Won-Young Lee, Hyun-Jung Park, Ran Lee, Ji-Heon Lee, Hyunjhung Jhun, Tai-Young Hur, Hyuk Song
      Spermatogenesis begins after puberty and continues throughout a male’s life, and is regulated by spermatogonial stem cells in the seminiferous tubules. Markers of male germ cells, including undifferentiated spermatogonia to fully developed spermatozoa have been identified in rodents, but not in dogs. In this study, to characterize the markers of undifferentiated spermatogonia, histological and immunohistochemical analyses were performed on pre-pubertal (1-month-old), early pubertal (4-month-old), and post-pubertal (7-month-old) dog testes. Expression of chemokine receptor 4 (CXCR4), insulin-like growth factor binding protein 3 (IGFBP3), LIN28, and Sal-like protein 4 (SALL4) genes was confirmed by immunohistochemical analysis. In pre-pubertal and early pubertal dog testes, CXCR4, IGFBP4, and LIN28 genes were expressed in undifferentiated spermatogonia, whereas the SALL4 gene was not expressed in the pre-pubertal stage. In adult dog testes, CXCR4 and IGFBP3 gene expression was detected in undifferentiated spermatogonia and co-localized with protein gene product 9.5 (PGP9.5) near the basement membrane of the seminiferous tubules. The LIN28 and SALL4 genes were expressed in synaptonemal complex protein 3-positive spermatocytes. The CXCR4 and IGFBP3 gene expression is conserved among other species, while LIN28 and SALL4 gene expression varies. Based on results of the present study, it is suggested that undifferentiated spermatogonia markers detected in other species are conserved in dogs. These results may facilitate further studies of the cellular mechanisms of spermatogenesis in dogs.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.017
  • Boar variability in sperm cryo-tolerance after cooling of semen in
           different long-term extenders at various temperatures
    • Authors: K. Wasilewska; L. Fraser
      Abstract: Publication date: Available online 24 August 2017
      Source:Animal Reproduction Science
      Author(s): K. Wasilewska, L. Fraser
      This study investigated individual boar variability in the quality of pre-freeze (PF) and post-thaw (PT) semen cooled in different long-term (LT) extenders and for different holding times (HT). Sperm rich fractions were diluted with Androhep® Plus (AHP), Androstar® Plus (ASP), Safecell® Plus (SCP) and TRIXcell® Plus (TCP) extenders, stored for 2h at 17°C (HT 1) and additionally for 24h at 10°C (HT 2) and the samples were subsequently evaluated and frozen. Besides the analysis of CASA sperm variables, mitochondrial membrane potential (MMP), plasma membrane integrity (PMI), normal apical ridge (NAR) acrosome integrity, and viability (YO-PRO-1−/PI−) of sperm were assessed in the PF and PT semen. Results indicated that boar, extender and HT group affected the sperm quality characteristics. There were great variations in PMOT and the sperm motion patterns of the PF semen among the boars. Differences in the HT groups of the PF semen, with respect to the sperm membrane integrity, were less marked among the boars. Consistent variations in TMOT and PMOT in the PT semen were observed among the boars, being greater in the HT 2 group. Most of the CASA-analyzed sperm motion patterns were greater in the HT 2 group of the PT semen. Furthermore, sperm MMP, PMI and viability were greater in the HT 2 group of the PT semen in most of the boars, while consistent differences were observed among the boars for sperm NAR acrosome integrity in either HT group. The significant effect of the cryopreservation process on the sperm membrane proteome was evident from the number of protein bands, detected in the electrophoretic profiles of sperm of the HT 1 and HT 2 groups. The electrophoretic profiles of the PF and PT semen among boars with poor and good semen freezability, however, differed with respect to the abundance and types of sperm membrane-associated proteins. The overall results of this study provided evidence that there are differences among boars in response to the different cooling regimens, and that cooling of extended semen for a 24-h period at 10°C modulated the functions of sperm in an extender-dependent manner, rendering the cells less susceptible to cryo-induced damage. It is suggested that the findings of this study have the potential to improve the technology of boar semen cryopreservation.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.016
  • Osmotic tolerance of feline epididymal spermatozoa
    • Authors: Panisara Kunkitti; Kaywalee Chatdarong; Junpen Suwimonteerabutr; Teerawut Nedumpun; Anders Johannisson; Ann-Sofi Bergqvist; Ylva Sjunnesson; Eva Axnér
      Abstract: Publication date: Available online 23 August 2017
      Source:Animal Reproduction Science
      Author(s): Panisara Kunkitti, Kaywalee Chatdarong, Junpen Suwimonteerabutr, Teerawut Nedumpun, Anders Johannisson, Ann-Sofi Bergqvist, Ylva Sjunnesson, Eva Axnér
      During the cryopreservation process, spermatozoa are exposed to hypertonic solutions contributed by the high concentration of cryoprotectant. During addition and removal of cryoprotectant the spermatozoa are subjected to a substantial osmotic stress. Spermatozoa of different species and different stages of maturation may have different susceptibility to osmotic stress depending on the biology of the cell membrane and this will affect their tolerance to the freezing-thawing stress. The aims of this study were to determine the osmotic tolerance limits for motility, membrane integrity and mitochondrial membrane potential of feline epididymal spermatozoa and to study the effect of osmotic stress on the feline spermatozoa of different epididymal regions. Epididymal spermatozoa from three regions (caput, corpus and cauda) were pre-exposed to various osmolalities (75, 300, 600, 900, 1200 mOsm) in a single step for 10min and returned to 300 mOsm afterward. Percentage of motile spermatozoa was measured subjectively and membrane integrity (SYBR-14 positive cells) was evaluated prior to and after exposure to different osmolalities. The mitochondrial membrane potential (JC1) of spermatozoa were evaluated using flow cytometer and compared between epididymal regions (caput, corpus and cauda). All the parameters were compared using a mixed procedure. The percentage of motile epididymal spermatozoa decreased significantly when spermatozoa were exposed to 75 mOsm and 600 mOsm. Epididymal spermatozoa showed signs of damage when pre-exposed to 900 and 1200 mOsm and returned to isotonic condition as significant reduction of membrane integrity and mitochondrial membrane potential were observed (P<0.05). The plasma membrane of spermatozoa from the cauda epididymal region showed higher susceptibility to osmotic stress than the other regions as demonstrated by a significant difference between regions after return to isotonicity from 900 mOsm (P>0.01) and a difference between caput and corpus after return from 1200 mOsm (P<0.05). The corpus and cauda epididymal spermatozoa had higher percentage of spermatozoa with high mitochondrial membrane potential than those from caput when exposed to 75, 300 and 600 mOsm (P<0.05). In conclusion, a single step exposure to hypertonic solution of greater than 600 mOsm prior to return to isotonic condition can cause severe damage to sperm membrane and mitochondrial membrane potential compared to non-returning (exposure to various osmolality but not returned to isotonic condition). Changes in osmolality impacted mostly on sperm motility. Spermatozoa from cauda epididymis were more susceptible to osmotic stress compared to those from corpus and caput indicating that the maturation changes in the sperm membrane during passage through the epididymis increase susceptibility to the osmotic changes that may occur during sperm cryopreservation.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.014
  • Irisin stimulates gonadotropins gene expression in tilapia (Oreochromis
           niloticus) pituitary cells
    • Authors: Quan Jiang; Qianli Zhang; Anji Lian; Yue Xu
      Abstract: Publication date: Available online 19 August 2017
      Source:Animal Reproduction Science
      Author(s): Quan Jiang, Qianli Zhang, Anji Lian, Yue Xu
      The link between energy metabolism and reproduction is well known in vertebrates. Irisin, the product of fibronectin type III domain-containing protein 5 (FNDC5) gene, plays an important role in energy homeostasis. However, biological actions of irisin on reproduction remain elusive. To address this gap, we examined the direct effects of irisin on luteinizing hormone β (LHβ) and follicle-stimulating hormone β (FSHβ) gene expression in tilapia pituitary cells. As a first step, the transcripts of FNDC5 were detected in the proximal pars distalis (PPD), but not in the rostral pars distalis (RPD) and neurointermediate lobe (NIL) of the tilapia pituitary by RT-PCR. In the tilapia pituitary, irisin immunoreactive signals were also detected in PPD region. In primary cultures of tilapia pituitary cells, irisin was effective in stimulating both LHβ and FSHβ mRNA levels in vivo and in vitro. In cultured pituitary cells of tilapia, removal of endogenous irisin by immunoneutralization using irisin antiserum inhibited LHβ and FSHβ gene expression. Salmon gonadotrophin releasing hormone (sGnRH) increased LHβ and FSHβ mRNA levels in tilapia pituitary but these stimulatory actions were not either enhanced by treatment with irisin or blocked by irisin antiserum. Furthermore, the stimulation on LHβ and FSHβ mRNA expression was coincident with the enhancement of LHβ and FSHβ mRNA stability after irisin treatment. These results provide evidence that irisin may serve as a novel intrapituitary factor maintaining gonadotropins gene expression in tilapia pituitary.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.06.018
  • Refining insulin concentrations in culture medium containing growth
           factors BMP15 and GDF9: an in vitro study of the effects on follicle
           development of goats
    • Authors: D.J. Dipaz-Berrocal; N.A.R. Sá; D.D. Guerreiro; J.J.H. Celestino; J. Leiva-Revilla; B.G.K.A. Alves; R.R. Santos; F.W.S. Cibin; A.P.R. Rodrigues; J.R. Figueiredo
      Abstract: Publication date: Available online 18 August 2017
      Source:Animal Reproduction Science
      Author(s): D.J. Dipaz-Berrocal, N.A.R. Sá, D.D. Guerreiro, J.J.H. Celestino, J. Leiva-Revilla, B.G.K.A. Alves, R.R. Santos, F.W.S. Cibin, A.P.R. Rodrigues, J.R. Figueiredo
      The aim of the present study was to investigate the influence of two insulin concentrations (10ng/mL and 10μg/mL) combined or in the absence of BMP15 and/or GDF9, on the in vitro survival and development of preantral follicles of goat ovarian tissue. Ovarian slices from the same goat ovary pair were randomly assigned to a non-cultured control treatment or to be in vitro cultured for 1 or 7days in α-MEM containing 10ng/mL (Low) or 10μg/mL (High) of insulin in the absence or presence of BMP15 and/or GDF9. With the low insulin treatment, there was a greater percentage of normal follicles than with the high insulin treatment. The addition of BMP15 alone or in association with GDF9 to the medium containing low insulin resulted in a lesser percentage of normal follicles (P< 0.05). The addition of BMP15 and GDF9 separately or in combination with the high insulin concentration enhanced the percentage of normal follicles. On day 7 of culture, the use of medium containing low insulin alone or high insulin supplemented with BMP15 and BMP15+GDF9 resulted in a greater percentage of secondary follicles than the non-cultured control, although follicles cultured with low insulin were smaller than those from the control group and had greater rates of oxidative stress. In conclusion, in the presence of physiological concentrations of insulin (10ng/mL), medium supplementation with GDF9 and BMP15 alone or in combination is unnecessary for normal follicle development in vitro.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.011
  • Delayed blastocyst formation or an extra day culture increases apoptosis
           in pig blastocysts
    • Authors: Tao Lin; Jae Eun Lee; Reza K. Oqani; So Yeon Kim; Eun Seok Cho; Yong Dae Jeong; Jun Jong Baek; Dong Il Jin
      Abstract: Publication date: Available online 18 August 2017
      Source:Animal Reproduction Science
      Author(s): Tao Lin, Jae Eun Lee, Reza K. Oqani, So Yeon Kim, Eun Seok Cho, Yong Dae Jeong, Jun Jong Baek, Dong Il Jin
      In the present study, the timing was examined of blastocyst collection/formation or of how the duration of post-blastulation culture affected the quality and developmental competence of in vitro-produced pig parthenogenetic embryos. The earliest apoptotic signals were observed at the morula stage while the earliest cytoplasmic fragmentation was observed before the 4- to 8-cell stage of embryo development. Nuclear condensation was detected in morulae and blastocysts, but not all condensed nuclei were positive for the apoptotic signal (TUNEL staining). The mean blastocyst diameter increased with delayed blastocyst collection or extended post-blastulation culture, but decreased with delayed blastocyst formation. Delayed blastocyst collection/formation or an additional day of post-blastulation culture increased the frequencies of apoptosis, condensed nuclei, and low quality blastocysts (those showing a nuclear destruction that negated counting of the nuclei); increased the expression of the pro-apoptotic BAX gene; and reduced the ratio of ICM (inner cell mass) cells to TE (trophectoderm) cells. In addition, delayed blastocyst formation decreased POU5F1 gene expression. These results suggest that a delay in blastocyst collection/formation or an additional day of culture could increase the incidence of apoptosis, decrease the ICM:TE cell ratio, and influence the gene expression and diameter of blastocysts derived from in vitro-produced pig embryos. These findings provide a useful reference for improving the quality of in vitro-produced embryos.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.012
  • Effects of estrus synchronization using Matrix® followed by treatment
           with the GnRH agonist triptorelin to control ovulation in mature gilts
    • Authors: R.V. Knox; S.K. Webel; M. Swanson; M.E. Johnston; R.R. Kraeling
      Abstract: Publication date: Available online 14 August 2017
      Source:Animal Reproduction Science
      Author(s): R.V. Knox, S.K. Webel, M. Swanson, M.E. Johnston, R.R. Kraeling
      Estrus and ovulation responses in Matrix-treated gilts may affect ovulation synchrony in response to triptorelin. In experiment 1, estrus and ovulation measures at 12h intervals after last Matrix feeding (LMF) were analyzed. For the 398 gilts that displayed estrus, 87.4% were detected on Days 6–8 after LMF. Duration of estrus was 24–60h for 85.6% of gilts and negatively correlated with interval from LMF to estrus (r=−0.53, P< 0.0001). The estrus to ovulation interval was positively correlated with duration of estrus (r=0.61, P< 0.0001). In experiment 2, gilts (n=96) received intravaginal treatment with 2mL of gel containing placebo (Control) at 96h, 200μg of triptorelin at 96h (TRP96), 120h (TRP120) or 144h (TRP144) after LMF. Estrus measures did not differ (P> 0.10) among treatments. The proportion of gilts ovulating 32–56h after treatment was greater for TRP120 and TRP144 (P< 0.01) compared to other treatments. The treatment to ovulation intervals for all triptorelin treatments were shorter (P< 0.001) than Control. In experiment 3, gilts (n=86) received placebo (Control), 100μg (TRP100), 200μg (TRP200), or 400μg (TRP400) of triptorelin at 120h after LMF. There was no effect of treatment (P> 0.10) on estrus or on interval from LMF to estrus. The proportion of gilts ovulating by 40, 48 and 56h after treatment increased (P< 0.05) with triptorelin compared to Control. Our results indicate that gilts receiving 100–400μg of triptorelin at 120h after LMF had the greatest ovulation synchrony 24–48h following treatment. These studies provide important information for developing a procedure for a single insemination in synchronized gilts.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.003
  • The association between subclinical mastitis around calving and
           reproductive performance in grazing dairy cows
    • Authors: N.A. Villa-Arcila; J. Sanchez; M.H. Ratto; J.C. Rodriguez-Lecompte; P.C. Duque-Madrid; S. Sanchez-Arias; A. Ceballos-Marquez
      Abstract: Publication date: Available online 14 August 2017
      Source:Animal Reproduction Science
      Author(s): N.A. Villa-Arcila, J. Sanchez, M.H. Ratto, J.C. Rodriguez-Lecompte, P.C. Duque-Madrid, S. Sanchez-Arias, A. Ceballos-Marquez
      The objective of this study was to evaluate the effect of subclinical mastitis (SCM) on calving-to-first-service interval (CFS), calving-to-conception interval (CC), and on the number of services per conception (S/C) in grazing Holstein and Normande cows. Primiparous (n=43) and multiparous (n=165) cows were selected from five dairy herds. Two composite milk samples were aseptically collected from each cow at drying-off, and then every week during the first postpartum month. One sample was used for somatic cell count (SCC), and the other one for bacteriological analysis. Cows were followed up to 300 d after calving. Non-parametric and parametric survival models, and negative binomial regression were used to assess the association between SCM, evaluated by SCC and milk culture, and reproductive indices. Staphylococcus aureus, CNS, and Streptococcus uberis were the most frequent isolated pathogens. Subclinical mastitis in the first month of lactation was not associated with CFS; however, the CC interval was longer in cows with SCM compared to healthy cows, the former also had a higher number of S/C.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.010
  • Expression of bone morphogenetic protein receptors in bovine oviductal
           epithelial cells: Evidence of autocrine BMP signaling
    • Authors: Pablo Alberto Valdecantos; Rocío del Carmen Bravo Miana; Elina Vanesa García; Daniela Celeste García; Mariela Roldán-Olarte; Dora Cristina Miceli
      Abstract: Publication date: Available online 14 August 2017
      Source:Animal Reproduction Science
      Author(s): Pablo Alberto Valdecantos, Rocío del Carmen Bravo Miana, Elina Vanesa García, Daniela Celeste García, Mariela Roldán-Olarte, Dora Cristina Miceli
      Members of the transforming growth factor beta (TGF-β) family, including bone morphogenetic proteins (BMPs), are expressed in the epithelial cells of the mammalian oviduct. These signaling molecules play important roles in development and tissue homeostasis; however, little is known about their function in the mammalian oviduct. In the present study, RT-qPCR was used to analyze the mRNA abundance of BMP type I (BMPR1A, BMPR1B, ACVR1) and type II receptors (BMPR2, ACVR2A, ACVR2B) in the bovine oviduct epithelial cells (BOEC) isolated from ampulla and isthmus at both the follicular (FP) and the luteal (LP) phase of the estrous cycle. Results indicate that mRNAs for all the BMP receptors studied are expressed in the BOEC. Significant mRNA abundance differences were observed for both BMPR1B and ACVR2B when comparing both the ampulla and isthmus regions with the greater abundance at the isthmus. When both FP and LP samples were compared, ACVR2B mRNA showed greater abundance during the LP, with significant differences in the isthmus region. These variations highlight differences between the isthmus and ampulla regions of the oviduct. By means of wound healing assays on BOEC primary cultures, exogenous recombinant human BMP5 induced a significant increase in wound healing at 24h. The observed changes at the mRNA abundance of components of the signaling pathway and the BMP5 effect on oviductal epithelial cells suggest a possible autocrine role for the BMP pathway that could affect epithelial cell functions necessary for normal physiology and reproductive success in BOEC homeostasis.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.006
  • Stallion sperm selection prior to freezing using a modified colloid
           swim-up procedure without centrifugation
    • Authors: M. Hidalgo; I. Ortiz; J. Dorado; J.M. Morrell; J. Gosálvez; C. Consuegra; M. Diaz-Jimenez; B. Pereira; F. Crespo
      Abstract: Publication date: Available online 12 August 2017
      Source:Animal Reproduction Science
      Author(s): M. Hidalgo, I. Ortiz, J. Dorado, J.M. Morrell, J. Gosálvez, C. Consuegra, M. Diaz-Jimenez, B. Pereira, F. Crespo
      The aims of this study were to: 1) develop a new method for stallion sperm selection using a modified swim-up procedure through a colloid and 2) evaluate its impact in good quality ejaculates from bad freezers in comparison to methods involving centrifugation such as single layer centrifugation and sperm washing. Ejaculates were processed before freezing using three different procedures: sperm washing (SW), colloid single layer centrifugation (SLC) and a modified colloid swim-up (SU). After semen processing, sperm recovery rates were measured and sperm were frozen. Post-thaw sperm motility (assessed by computer-assisted sperm analysis), normal forms and plasma membrane integrity (evaluated under bright-field and fluorescence microscopy respectively), and DNA fragmentation (assessed by the Sperm-Halomax kit) were compared between treatments. Sperm recovery rates were similar between SU and SLC but lower than SW. Sperm motility after thawing was lower in SU in comparison to SLC and SW, maybe due to the incomplete removal of seminal plasma before freezing. Sperm DNA fragmentation was lower in SU and SLC selection methods, particularly in SLC selected samples during the first 6h of incubation. The remaining sperm parameters assessed were similar among treatments. In conclusion, SLC is more suitable than SW and SU to process stallion semen prior to freezing, in particular when sperm DNA damage is suspected. Further studies are needed in order to determine the potential benefits of SU in samples where centrifugation is not necessary, such as epididymal sperm, ejaculate fractioning or post-thaw semen samples.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.005
  • Melatonin treatment in winter and spring and reproductive recovery in
           Sarda breed sheep
    • Authors: Maria Consuelo Mura; Sebastiano Luridiana; Federico Farci; Maria Veronica Di Stefano; Cinzia Daga; Luisa Pulinas; Jože Starič; Vincenzo Carcangiu
      Abstract: Publication date: Available online 12 August 2017
      Source:Animal Reproduction Science
      Author(s): Maria Consuelo Mura, Sebastiano Luridiana, Federico Farci, Maria Veronica Di Stefano, Cinzia Daga, Luisa Pulinas, Jože Starič, Vincenzo Carcangiu
      This study aimed to evaluate the effect of melatonin treatment carried out between late winter and early spring on reproductive response in Sarda breed sheep and whether the photo-refractoriness can influence this reproductive response. The study was conducted on 3200 adult ewes, aged 3–6years old, with body condition score (BCS) 2.5–4.0, from 16 commercial sheep farms in Northern Sardinia. In each farm 200 animals were enrolled and subdivided into 2 groups (n=100 each): Group M (treated with one 18mg melatonin implant), and group C (untreated). The melatonin treatments were performed on 10th February; 10th March; 10th April and on 10th May each time in 4 different randomly selected farms. Adult males, treated with 3 melatonin implants 1 week before females, were introduced in each flock 35days after ewes’ treatment, and removed after 45days of cohabitation with females. Pregnancy was determined by transabdominal ultrasonography examination between 45th and 90th day after ram introduction. Data showed that melatonin treatment increased the fertility rate significantly (P<0.05), with the higher fertility rate in the ewes treated in April and May. The average time in days from male introduction to lambing was shorter in treated than in control ewes (P<0.05). Further, at the 160th and 170th day after male introduction the group M showed a higher number of lambed ewes compared to C (P<0.01). This effect was observed at 180th and 190th days after ram introduction, also, but with lower significance (P<0.05). In conclusion, melatonin treatment improved reproductive efficiency and advanced breeding season in Sarda sheep, especially when ewes were treated in spring.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.009
  • Relationship between time post-ovulation and progesterone on oocyte
           maturation and pregnancy in canine cloning
    • Authors: Joung Joo Kim; Kang Bae Park; Eun Ji Choi; Sang Hwan Hyun; Nam-Hyung Kim; Yeon Woo Jeong; Woo Suk Hwang
      Abstract: Publication date: Available online 12 August 2017
      Source:Animal Reproduction Science
      Author(s): Joung Joo Kim, Kang Bae Park, Eun Ji Choi, Sang Hwan Hyun, Nam-Hyung Kim, Yeon Woo Jeong, Woo Suk Hwang
      Canine oocytes ovulated at prophase complete meiosis and continue to develop in presence of a high progesterone concentration in the oviduct. Considering that meiotic competence of canine oocyte is accomplished in the oviductal environment, we postulate that hormonal milieu resulting from the circulating progesterone concentration may affect oocyte maturation and early development of embryos. From 237 oocyte donors, 2620 oocytes were collected and their meiotic status and morphology were determined. To determine optimal characteristics of the mature oocytes subjected to nuclear transfer, a proportion of the meiotic status of the oocytes were classified in reference to time post-ovulation as well as progesterone (P4) level. A high proportion of matured oocytes were collected from >126h (55.5%) post-ovulation or 40–50ngmL−1 (46.4%) group compared to the other groups. Of the oocyte donors that provided mature oocytes in vivo, there was no correlation between serum progesterone of donors and time post ovulation, however, time post-ovulation were significantly shorter for <30ng/mL group (P<0.05). Using mature oocytes, 1161 cloned embryos were reconstructed and transferred into 77 surrogates. In order to determine the relationship between pregnancy performance and serum progesterone level, embryos were transferred into surrogates showing various P4 serum levels. The highest pregnancy (31.8%) and live birth cloning efficacy (2.2%) rates were observed when the embryos were transferred into surrogates with circulating P4 levels were from 40 to 50ngmL−1. In conclusion, measurement of circulating progesterone of female dog could be a suitable an indicator of the optimal time to collect quality oocyte and to select surrogates for cloning.

      PubDate: 2017-09-02T04:56:16Z
      DOI: 10.1016/j.anireprosci.2017.08.004
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