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Publisher: Elsevier   (Total: 3181 journals)

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Showing 1 - 200 of 3181 Journals sorted alphabetically
Academic Pediatrics     Hybrid Journal   (Followers: 39, SJR: 1.655, CiteScore: 2)
Academic Radiology     Hybrid Journal   (Followers: 26, SJR: 1.015, CiteScore: 2)
Accident Analysis & Prevention     Partially Free   (Followers: 105, SJR: 1.462, CiteScore: 3)
Accounting Forum     Hybrid Journal   (Followers: 28, SJR: 0.932, CiteScore: 2)
Accounting, Organizations and Society     Hybrid Journal   (Followers: 42, SJR: 1.771, CiteScore: 3)
Achievements in the Life Sciences     Open Access   (Followers: 7)
Acta Anaesthesiologica Taiwanica     Open Access   (Followers: 6)
Acta Astronautica     Hybrid Journal   (Followers: 443, SJR: 0.758, CiteScore: 2)
Acta Automatica Sinica     Full-text available via subscription   (Followers: 2)
Acta Biomaterialia     Hybrid Journal   (Followers: 29, SJR: 1.967, CiteScore: 7)
Acta Colombiana de Cuidado Intensivo     Full-text available via subscription   (Followers: 3)
Acta de Investigación Psicológica     Open Access   (Followers: 3)
Acta Ecologica Sinica     Open Access   (Followers: 11, SJR: 0.18, CiteScore: 1)
Acta Histochemica     Hybrid Journal   (Followers: 5, SJR: 0.661, CiteScore: 2)
Acta Materialia     Hybrid Journal   (Followers: 319, SJR: 3.263, CiteScore: 6)
Acta Mathematica Scientia     Full-text available via subscription   (Followers: 5, SJR: 0.504, CiteScore: 1)
Acta Mechanica Solida Sinica     Full-text available via subscription   (Followers: 9, SJR: 0.542, CiteScore: 1)
Acta Oecologica     Hybrid Journal   (Followers: 12, SJR: 0.834, CiteScore: 2)
Acta Otorrinolaringologica (English Edition)     Full-text available via subscription  
Acta Otorrinolaringológica Española     Full-text available via subscription   (Followers: 2, SJR: 0.307, CiteScore: 0)
Acta Pharmaceutica Sinica B     Open Access   (Followers: 2, SJR: 1.793, CiteScore: 6)
Acta Poética     Open Access   (Followers: 4, SJR: 0.101, CiteScore: 0)
Acta Psychologica     Hybrid Journal   (Followers: 26, SJR: 1.331, CiteScore: 2)
Acta Sociológica     Open Access   (Followers: 1)
Acta Tropica     Hybrid Journal   (Followers: 6, SJR: 1.052, CiteScore: 2)
Acta Urológica Portuguesa     Open Access  
Actas Dermo-Sifiliograficas     Full-text available via subscription   (Followers: 3, SJR: 0.374, CiteScore: 1)
Actas Dermo-Sifiliográficas (English Edition)     Full-text available via subscription   (Followers: 2)
Actas Urológicas Españolas     Full-text available via subscription   (Followers: 3, SJR: 0.344, CiteScore: 1)
Actas Urológicas Españolas (English Edition)     Full-text available via subscription   (Followers: 1)
Actualites Pharmaceutiques     Full-text available via subscription   (Followers: 7, SJR: 0.19, CiteScore: 0)
Actualites Pharmaceutiques Hospitalieres     Full-text available via subscription   (Followers: 3)
Acupuncture and Related Therapies     Hybrid Journal   (Followers: 8)
Acute Pain     Full-text available via subscription   (Followers: 15, SJR: 2.671, CiteScore: 5)
Ad Hoc Networks     Hybrid Journal   (Followers: 11, SJR: 0.53, CiteScore: 4)
Addictive Behaviors     Hybrid Journal   (Followers: 18, SJR: 1.29, CiteScore: 3)
Addictive Behaviors Reports     Open Access   (Followers: 9, SJR: 0.755, CiteScore: 2)
Additive Manufacturing     Hybrid Journal   (Followers: 11, SJR: 2.611, CiteScore: 8)
Additives for Polymers     Full-text available via subscription   (Followers: 23)
Advanced Drug Delivery Reviews     Hybrid Journal   (Followers: 187, SJR: 4.09, CiteScore: 13)
Advanced Engineering Informatics     Hybrid Journal   (Followers: 12, SJR: 1.167, CiteScore: 4)
Advanced Powder Technology     Hybrid Journal   (Followers: 17, SJR: 0.694, CiteScore: 3)
Advances in Accounting     Hybrid Journal   (Followers: 9, SJR: 0.277, CiteScore: 1)
Advances in Agronomy     Full-text available via subscription   (Followers: 17, SJR: 2.384, CiteScore: 5)
Advances in Anesthesia     Full-text available via subscription   (Followers: 30, SJR: 0.126, CiteScore: 0)
Advances in Antiviral Drug Design     Full-text available via subscription   (Followers: 2)
Advances in Applied Mathematics     Full-text available via subscription   (Followers: 12, SJR: 0.992, CiteScore: 1)
Advances in Applied Mechanics     Full-text available via subscription   (Followers: 12, SJR: 1.551, CiteScore: 4)
Advances in Applied Microbiology     Full-text available via subscription   (Followers: 24, SJR: 2.089, CiteScore: 5)
Advances In Atomic, Molecular, and Optical Physics     Full-text available via subscription   (Followers: 15, SJR: 0.572, CiteScore: 2)
Advances in Biological Regulation     Hybrid Journal   (Followers: 4, SJR: 2.61, CiteScore: 7)
Advances in Botanical Research     Full-text available via subscription   (Followers: 2, SJR: 0.686, CiteScore: 2)
Advances in Cancer Research     Full-text available via subscription   (Followers: 34, SJR: 3.043, CiteScore: 6)
Advances in Carbohydrate Chemistry and Biochemistry     Full-text available via subscription   (Followers: 9, SJR: 1.453, CiteScore: 2)
Advances in Catalysis     Full-text available via subscription   (Followers: 5, SJR: 1.992, CiteScore: 5)
Advances in Cell Aging and Gerontology     Full-text available via subscription   (Followers: 5)
Advances in Cellular and Molecular Biology of Membranes and Organelles     Full-text available via subscription   (Followers: 14)
Advances in Chemical Engineering     Full-text available via subscription   (Followers: 29, SJR: 0.156, CiteScore: 1)
Advances in Child Development and Behavior     Full-text available via subscription   (Followers: 11, SJR: 0.713, CiteScore: 1)
Advances in Chronic Kidney Disease     Full-text available via subscription   (Followers: 10, SJR: 1.316, CiteScore: 2)
Advances in Clinical Chemistry     Full-text available via subscription   (Followers: 26, SJR: 1.562, CiteScore: 3)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 20, SJR: 1.977, CiteScore: 8)
Advances in Computers     Full-text available via subscription   (Followers: 14, SJR: 0.205, CiteScore: 1)
Advances in Dermatology     Full-text available via subscription   (Followers: 15)
Advances in Developmental Biology     Full-text available via subscription   (Followers: 13)
Advances in Digestive Medicine     Open Access   (Followers: 12)
Advances in DNA Sequence-Specific Agents     Full-text available via subscription   (Followers: 7)
Advances in Drug Research     Full-text available via subscription   (Followers: 26)
Advances in Ecological Research     Full-text available via subscription   (Followers: 44, SJR: 2.524, CiteScore: 4)
Advances in Engineering Software     Hybrid Journal   (Followers: 29, SJR: 1.159, CiteScore: 4)
Advances in Experimental Biology     Full-text available via subscription   (Followers: 8)
Advances in Experimental Social Psychology     Full-text available via subscription   (Followers: 52, SJR: 5.39, CiteScore: 8)
Advances in Exploration Geophysics     Full-text available via subscription   (Followers: 1)
Advances in Fluorine Science     Full-text available via subscription   (Followers: 9)
Advances in Food and Nutrition Research     Full-text available via subscription   (Followers: 67, SJR: 0.591, CiteScore: 2)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 17)
Advances in Genetics     Full-text available via subscription   (Followers: 21, SJR: 1.354, CiteScore: 4)
Advances in Genome Biology     Full-text available via subscription   (Followers: 11, SJR: 12.74, CiteScore: 13)
Advances in Geophysics     Full-text available via subscription   (Followers: 7, SJR: 1.193, CiteScore: 3)
Advances in Heat Transfer     Full-text available via subscription   (Followers: 26, SJR: 0.368, CiteScore: 1)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 11, SJR: 0.749, CiteScore: 3)
Advances in Human Factors/Ergonomics     Full-text available via subscription   (Followers: 26)
Advances in Imaging and Electron Physics     Full-text available via subscription   (Followers: 3, SJR: 0.193, CiteScore: 0)
Advances in Immunology     Full-text available via subscription   (Followers: 37, SJR: 4.433, CiteScore: 6)
Advances in Inorganic Chemistry     Full-text available via subscription   (Followers: 10, SJR: 1.163, CiteScore: 2)
Advances in Insect Physiology     Full-text available via subscription   (Followers: 2, SJR: 1.938, CiteScore: 3)
Advances in Integrative Medicine     Hybrid Journal   (Followers: 6, SJR: 0.176, CiteScore: 0)
Advances in Intl. Accounting     Full-text available via subscription   (Followers: 3)
Advances in Life Course Research     Hybrid Journal   (Followers: 9, SJR: 0.682, CiteScore: 2)
Advances in Lipobiology     Full-text available via subscription   (Followers: 1)
Advances in Magnetic and Optical Resonance     Full-text available via subscription   (Followers: 8)
Advances in Marine Biology     Full-text available via subscription   (Followers: 21, SJR: 0.88, CiteScore: 2)
Advances in Mathematics     Full-text available via subscription   (Followers: 15, SJR: 3.027, CiteScore: 2)
Advances in Medical Sciences     Hybrid Journal   (Followers: 8, SJR: 0.694, CiteScore: 2)
Advances in Medicinal Chemistry     Full-text available via subscription   (Followers: 6)
Advances in Microbial Physiology     Full-text available via subscription   (Followers: 5, SJR: 1.158, CiteScore: 3)
Advances in Molecular and Cell Biology     Full-text available via subscription   (Followers: 25)
Advances in Molecular and Cellular Endocrinology     Full-text available via subscription   (Followers: 8)
Advances in Molecular Toxicology     Full-text available via subscription   (Followers: 7, SJR: 0.182, CiteScore: 0)
Advances in Nanoporous Materials     Full-text available via subscription   (Followers: 5)
Advances in Oncobiology     Full-text available via subscription   (Followers: 2)
Advances in Organ Biology     Full-text available via subscription   (Followers: 2)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 18, SJR: 1.875, CiteScore: 4)
Advances in Parallel Computing     Full-text available via subscription   (Followers: 7, SJR: 0.174, CiteScore: 0)
Advances in Parasitology     Full-text available via subscription   (Followers: 5, SJR: 1.579, CiteScore: 4)
Advances in Pediatrics     Full-text available via subscription   (Followers: 27, SJR: 0.461, CiteScore: 1)
Advances in Pharmaceutical Sciences     Full-text available via subscription   (Followers: 19)
Advances in Pharmacology     Full-text available via subscription   (Followers: 17, SJR: 1.536, CiteScore: 3)
Advances in Physical Organic Chemistry     Full-text available via subscription   (Followers: 9, SJR: 0.574, CiteScore: 1)
Advances in Phytomedicine     Full-text available via subscription  
Advances in Planar Lipid Bilayers and Liposomes     Full-text available via subscription   (Followers: 3, SJR: 0.109, CiteScore: 1)
Advances in Plant Biochemistry and Molecular Biology     Full-text available via subscription   (Followers: 10)
Advances in Plant Pathology     Full-text available via subscription   (Followers: 6)
Advances in Porous Media     Full-text available via subscription   (Followers: 5)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 19)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 20, SJR: 0.791, CiteScore: 2)
Advances in Psychology     Full-text available via subscription   (Followers: 68)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 6, SJR: 0.371, CiteScore: 1)
Advances in Radiation Oncology     Open Access   (Followers: 2, SJR: 0.263, CiteScore: 1)
Advances in Small Animal Medicine and Surgery     Hybrid Journal   (Followers: 3, SJR: 0.101, CiteScore: 0)
Advances in Space Biology and Medicine     Full-text available via subscription   (Followers: 6)
Advances in Space Research     Full-text available via subscription   (Followers: 423, SJR: 0.569, CiteScore: 2)
Advances in Structural Biology     Full-text available via subscription   (Followers: 5)
Advances in Surgery     Full-text available via subscription   (Followers: 13, SJR: 0.555, CiteScore: 2)
Advances in the Study of Behavior     Full-text available via subscription   (Followers: 38, SJR: 2.208, CiteScore: 4)
Advances in Veterinary Medicine     Full-text available via subscription   (Followers: 20)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 15)
Advances in Virus Research     Full-text available via subscription   (Followers: 6, SJR: 2.262, CiteScore: 5)
Advances in Water Resources     Hybrid Journal   (Followers: 54, SJR: 1.551, CiteScore: 3)
Aeolian Research     Hybrid Journal   (Followers: 6, SJR: 1.117, CiteScore: 3)
Aerospace Science and Technology     Hybrid Journal   (Followers: 383, SJR: 0.796, CiteScore: 3)
AEU - Intl. J. of Electronics and Communications     Hybrid Journal   (Followers: 8, SJR: 0.42, CiteScore: 2)
African J. of Emergency Medicine     Open Access   (Followers: 6, SJR: 0.296, CiteScore: 0)
Ageing Research Reviews     Hybrid Journal   (Followers: 12, SJR: 3.671, CiteScore: 9)
Aggression and Violent Behavior     Hybrid Journal   (Followers: 482, SJR: 1.238, CiteScore: 3)
Agri Gene     Hybrid Journal   (Followers: 1, SJR: 0.13, CiteScore: 0)
Agricultural and Forest Meteorology     Hybrid Journal   (Followers: 18, SJR: 1.818, CiteScore: 5)
Agricultural Systems     Hybrid Journal   (Followers: 31, SJR: 1.156, CiteScore: 4)
Agricultural Water Management     Hybrid Journal   (Followers: 44, SJR: 1.272, CiteScore: 3)
Agriculture and Agricultural Science Procedia     Open Access   (Followers: 4)
Agriculture and Natural Resources     Open Access   (Followers: 3)
Agriculture, Ecosystems & Environment     Hybrid Journal   (Followers: 58, SJR: 1.747, CiteScore: 4)
Ain Shams Engineering J.     Open Access   (Followers: 5, SJR: 0.589, CiteScore: 3)
Air Medical J.     Hybrid Journal   (Followers: 8, SJR: 0.26, CiteScore: 0)
AKCE Intl. J. of Graphs and Combinatorics     Open Access   (SJR: 0.19, CiteScore: 0)
Alcohol     Hybrid Journal   (Followers: 12, SJR: 1.153, CiteScore: 3)
Alcoholism and Drug Addiction     Open Access   (Followers: 12)
Alergologia Polska : Polish J. of Allergology     Full-text available via subscription   (Followers: 1)
Alexandria Engineering J.     Open Access   (Followers: 2, SJR: 0.604, CiteScore: 3)
Alexandria J. of Medicine     Open Access   (Followers: 1, SJR: 0.191, CiteScore: 1)
Algal Research     Partially Free   (Followers: 11, SJR: 1.142, CiteScore: 4)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 2)
Allergologia et Immunopathologia     Full-text available via subscription   (Followers: 1, SJR: 0.504, CiteScore: 1)
Allergology Intl.     Open Access   (Followers: 5, SJR: 1.148, CiteScore: 2)
Alpha Omegan     Full-text available via subscription   (SJR: 3.521, CiteScore: 6)
ALTER - European J. of Disability Research / Revue Européenne de Recherche sur le Handicap     Full-text available via subscription   (Followers: 11, SJR: 0.201, CiteScore: 1)
Alzheimer's & Dementia     Hybrid Journal   (Followers: 54, SJR: 4.66, CiteScore: 10)
Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring     Open Access   (Followers: 6, SJR: 1.796, CiteScore: 4)
Alzheimer's & Dementia: Translational Research & Clinical Interventions     Open Access   (Followers: 6, SJR: 1.108, CiteScore: 3)
Ambulatory Pediatrics     Hybrid Journal   (Followers: 5)
American Heart J.     Hybrid Journal   (Followers: 58, SJR: 3.267, CiteScore: 4)
American J. of Cardiology     Hybrid Journal   (Followers: 66, SJR: 1.93, CiteScore: 3)
American J. of Emergency Medicine     Hybrid Journal   (Followers: 47, SJR: 0.604, CiteScore: 1)
American J. of Geriatric Pharmacotherapy     Full-text available via subscription   (Followers: 13)
American J. of Geriatric Psychiatry     Hybrid Journal   (Followers: 14, SJR: 1.524, CiteScore: 3)
American J. of Human Genetics     Hybrid Journal   (Followers: 37, SJR: 7.45, CiteScore: 8)
American J. of Infection Control     Hybrid Journal   (Followers: 29, SJR: 1.062, CiteScore: 2)
American J. of Kidney Diseases     Hybrid Journal   (Followers: 36, SJR: 2.973, CiteScore: 4)
American J. of Medicine     Hybrid Journal   (Followers: 50)
American J. of Medicine Supplements     Full-text available via subscription   (Followers: 3, SJR: 1.967, CiteScore: 2)
American J. of Obstetrics and Gynecology     Hybrid Journal   (Followers: 267, SJR: 2.7, CiteScore: 4)
American J. of Ophthalmology     Hybrid Journal   (Followers: 66, SJR: 3.184, CiteScore: 4)
American J. of Ophthalmology Case Reports     Open Access   (Followers: 5, SJR: 0.265, CiteScore: 0)
American J. of Orthodontics and Dentofacial Orthopedics     Full-text available via subscription   (Followers: 6, SJR: 1.289, CiteScore: 1)
American J. of Otolaryngology     Hybrid Journal   (Followers: 25, SJR: 0.59, CiteScore: 1)
American J. of Pathology     Hybrid Journal   (Followers: 32, SJR: 2.139, CiteScore: 4)
American J. of Preventive Medicine     Hybrid Journal   (Followers: 28, SJR: 2.164, CiteScore: 4)
American J. of Surgery     Hybrid Journal   (Followers: 39, SJR: 1.141, CiteScore: 2)
American J. of the Medical Sciences     Hybrid Journal   (Followers: 12, SJR: 0.767, CiteScore: 1)
Ampersand : An Intl. J. of General and Applied Linguistics     Open Access   (Followers: 7)
Anaerobe     Hybrid Journal   (Followers: 4, SJR: 1.144, CiteScore: 3)
Anaesthesia & Intensive Care Medicine     Full-text available via subscription   (Followers: 67, SJR: 0.138, CiteScore: 0)
Anaesthesia Critical Care & Pain Medicine     Full-text available via subscription   (Followers: 25, SJR: 0.411, CiteScore: 1)
Anales de Cirugia Vascular     Full-text available via subscription   (Followers: 1)
Anales de Pediatría     Full-text available via subscription   (Followers: 3, SJR: 0.277, CiteScore: 0)
Anales de Pediatría (English Edition)     Full-text available via subscription  
Anales de Pediatría Continuada     Full-text available via subscription  
Analytic Methods in Accident Research     Hybrid Journal   (Followers: 5, SJR: 4.849, CiteScore: 10)
Analytica Chimica Acta     Hybrid Journal   (Followers: 44, SJR: 1.512, CiteScore: 5)
Analytica Chimica Acta : X     Open Access  
Analytical Biochemistry     Hybrid Journal   (Followers: 210, SJR: 0.633, CiteScore: 2)
Analytical Chemistry Research     Open Access   (Followers: 13, SJR: 0.411, CiteScore: 2)
Analytical Spectroscopy Library     Full-text available via subscription   (Followers: 14)
Anesthésie & Réanimation     Full-text available via subscription   (Followers: 2)
Anesthesiology Clinics     Full-text available via subscription   (Followers: 25, SJR: 0.683, CiteScore: 2)
Angiología     Full-text available via subscription   (SJR: 0.121, CiteScore: 0)
Angiologia e Cirurgia Vascular     Open Access   (Followers: 1, SJR: 0.111, CiteScore: 0)
Animal Behaviour     Hybrid Journal   (Followers: 226, SJR: 1.58, CiteScore: 3)
Animal Feed Science and Technology     Hybrid Journal   (Followers: 7, SJR: 0.937, CiteScore: 2)
Animal Reproduction Science     Hybrid Journal   (Followers: 7, SJR: 0.704, CiteScore: 2)

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Similar Journals
Journal Cover
Animal Reproduction Science
Journal Prestige (SJR): 0.704
Citation Impact (citeScore): 2
Number of Followers: 7  
 
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 0378-4320
Published by Elsevier Homepage  [3181 journals]
  • Cervical penetration rates and efficiency of non-surgical embryo recovery
           in estrous-synchronized Santa Inês ewes after administration of estradiol
           ester (benzoate or cypionate) in combination with d-cloprostenol and
           oxytocin
    • Abstract: Publication date: April 2019Source: Animal Reproduction Science, Volume 203Author(s): J.F. Fonseca, F.N. Zambrini, J.D. Guimarães, M.R. Silva, M.E.F. Oliveira, P.M. Bartlewski, J.M.G. Souza-Fabjan The effects of estradiol esters, d-cloprostenol and oxytocin on induction of cervical dilation prior to non-surgical embryo recovery in Santa Inês ewes (Days 6–7 estrous cycle) were assessed in this study. In Trial 1, transcervical embryo flushing was performed in estrous-induced ewes administered 37.5 μg of d-cloprostenol i.m. 10 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing with (EB-PGF-OT; n = 13) or without (PGF-OT; n = 11) 1 mg of estradiol benzoate i.m. administered concurrently with d-cloprostenol injection. In Trial 2, the estrous-synchronized animals were treated with 1 mg of estradiol benzoate (EB-PGF-OT; n = 12) or estradiol cypionate (EC-PGF-OT; n = 12) i.m. along with 37.5 μg of d-cloprostenol i.m. 16 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing. In Trial 1, uterine flushing could be accomplished in 38% of ewes in the EB-PGF-OT and 27% those in the PGF-OT (P>0.05) group. Flushing fluid recovery averaged 90% and there were 1.0 ± 1.1 embryos/ewe collected with mean duration of the flushing procedure being ˜36 min. In Trial 2, uterine flushing was accomplished in 78% of ewes in the EB-PGF-OT and 44% of those in the EC-PGF-OT group (P>0.05) with mean flushing fluid recovery rate being 88% and time elapsing to complete flushing being ˜33 min. Within the subsets of animals treated with EB, the percentages of successful transcervical penetrations were 38% compared with 78% in Trials 1 and 2, respectively (i.e., with EB administered 10 h compared with 16 h before uterine flushing: P
       
  • Characterization of fertility associated sperm proteins in Aseel and Rhode
           Island Red chicken breeds
    • Abstract: Publication date: April 2019Source: Animal Reproduction Science, Volume 203Author(s): Gurjot Kaur Mavi, P.P. Dubey, Ranjna S. Cheema, B.K. Bansal This study focused on characterization of fertility associated proteins in Aseel and RIR roosters and was conducted on two generations of birds. Roosters were divided into high (>50%) and low fertility groups (
       
  • Comparative studies on testis, epididymis and serum hormone concentrations
           in foxes, and hybrids during the pre-breeding period
    • Abstract: Publication date: April 2019Source: Animal Reproduction Science, Volume 203Author(s): T.A. Yang, Y.H. Yang, X.C. Song, L.L. Liu, Y.F. Yang, X.M. Xing, F.H. Yang, Y.H. Peng Silver fox and blue fox belong to different genera, and the hybrid males are reproductively sterile. In the present study, there was a comparison of testicular and epididymal morphology and serum hormone concentrations among silver foxes, blue foxes, and the hybrids during the pre-breeding period, using 20 male silver foxes, 20 male blue foxes, 15 male HSBs (silver fox male × blue fox female hybrids) and 15 male HBSs (blue fox male × silver fox female hybrids), respectively. Hybrids had a smaller diameter of seminiferous tubules than pure-species males, and testes of hybrid males did not differ in mean size and relative weight from pure-species males. There were many Sertoli cells and spermatogenic cells in silver foxes and blue foxes, while numbers of spermatogonia and primary spermatocytes were less with no secondary spermatocytes in the hybrids. Furthermore, mean serum testosterone and estradiol concentrations in the hybrids were less, and FSH, LH, and PRL were greater than that in silver foxes and blue foxes (P 
       
  • Soybean isoflavone affects in rabbits: Effects on metabolism, antioxidant
           capacity, hormonal balance and reproductive performance
    • Abstract: Publication date: April 2019Source: Animal Reproduction Science, Volume 203Author(s): M.A. Abo-elsoud, N.M. Hashem, A.N.M. Nour El-Din, K.I. Kamel, G.A. Hassan Though soybean isoflavones (SBI) have pharmaceutical properties, the compounds also have endocrine disrupting activities that may adversely affect fertility of mammals. The effects of SBI on metabolism, antioxidant capacity, hormonal balance and reproductive performance of male rabbits were investigated. Adult male rabbits (n = 21) fed an isoflavone-free diet were orally treated with 0 (control; CON), 5 (small; LSBI) or 20 (large; HSBI) mg of SBI/kg body weight/day for 12 weeks. Both SBI doses resulted in lesser blood plasma total protein concentrations, while there were no effects on glucose and cholesterol concentrations compared to CON. The HSBI-treated males had the greatest (P 
       
  • Effect of dietary supplementation with long-chain n-3 fatty acids during
           late gestation and early lactation on mare and foal plasma fatty acid
           
    • Abstract: Publication date: April 2019Source: Animal Reproduction Science, Volume 203Author(s): J.M. Kouba, T.A. Burns, S.K. Webel The effects of dietary marine-derived n-3 fatty acids (FA) on mare milk and mare and foal plasma FA, postpartum ovarian follicular growth and prostaglandin concentrations were evaluated. Sixty days prior to expected foaling dates, mares were assigned to one of three diets: corn oil (CORN, n = 6), docosahexaenoic acid (DHA) diet (D; 12.64 g/d, n = 7), or eicosapentaenoic acid (EPA) and DHA (ED; 8.84 g EPA, 10.43 g DHA/d, n = 7). Milk and plasma were collected for FA analysis. Follicular data were recorded through the first postpartum ovulation. Post-ovulation serial blood samples were evaluated for prostaglandin F2α metabolite (PGFM). Supplementation with DHA, or DHA plus EPA resulted in lower linoleic acid and greater EPA and DHA in mare plasma (P 
       
  • Effect of sex ratio, storage time and temperature on hatching rate,
           fertility and embryonic mortality in Chukar partridge (Alectoris chukar)
    • Abstract: Publication date: April 2019Source: Animal Reproduction Science, Volume 203Author(s): Hassan Habibi, Najmeh Ghahtan, Daniel M. Brooks Chukar partridges (Alectoris chukar) are frequently reared in captivity with the aim of producing fertile eggs and chicks. The aims of the present study were to evaluate the effect of male to female sex ratio on hatching rate and fertility and to determine the optimal temperature and time for egg storage and its effect on hatching rate and embryonic mortality in Chukar partridges. Maximum hatching rate and fertility rate were affected by male:female ratios during breeding (P 
       
  • Analyses of mathematical models for Yangzhou geese egg-laying curves
    • Abstract: Publication date: April 2019Source: Animal Reproduction Science, Volume 203Author(s): Binbin Guo, Sanqin Zhao, Xibing Shao, Weimin Ding, Zhendan Shi, Zhongliang Tang Mathematical models of the egg-laying curves for Yangzhou geese exposed to both natural and artificial photoperiods were established to optimise the parameters for maximising geese reproductive performance and for the development of precision feeding methods. With the natural photoperiod, egg-laying starts in autumn when daily photoperiod decreases, but accelerates after the winter solstice, and reaches the peak in spring when photoperiod increases. An accumulating model was constructed based on the hypothesis that the egg-laying capacity of geese was determined by two components of the photoperiod: photo-stimulation and photo-inhibition. In addition, a second segmented model was constructed based on the hypothesis that the photo-stimulation only occurred with lengthening photoperiods after the winter solstice, and the lesser laying rate in autumn could be attributed to the non-photo-dependent animal-husbandry technologies. This model consists of a logistic model before the winter solstice, and an accumulating model after this solstice. The use of the logistic and accumulating resulted in more precise predictions that occurred with use of Model 1 with a greater R2 and lesser RMSE, AIC and BIC. Likewise, the egg-laying curves when there was consideration of artificial photoperiods could also be constructed with consideration of stimulatory and inhibitory photoperiodic effects. The model consists of an initial logistic and subsequently a quadratic polynomial model. With use of this model, there is consideration of changes in egg-laying patterns when there is a fixed photoperiod, with the model parameters reflecting the effects by photoperiod control-programs and age of the geese. In conclusion, new mathematical models have been developed to best fit egg-laying curves when there are both natural and artificial photoperiods. These models can contribute to development of precision-feeding technologies for breeding geese in future.
       
  • In vitro effects of tongue sole LPXRFa and kisspeptin on relative
           abundance of pituitary hormone mRNA and inhibitory action of LPXRFa on
           kisspeptin activation in the PKC pathway
    • Abstract: Publication date: April 2019Source: Animal Reproduction Science, Volume 203Author(s): Bin Wang, Guokun Yang, Yongjiang Xu, Yaxing Zhang, Xuezhou Liu Results of previous studies indicated the existence of LPXRFa, the piscine ortholog of gonadotropin-inhibitory hormone (GnIH), and kisspeptin (Kiss2) in tongue sole (Cynoglossus semilaevis), and that LPXRFa exerts an inhibitory effect on Kiss2 activation in the protein kinase A (PKA) pathway. The functions in the control of reproduction and whether LPXRFa antagonizes the action of Kiss2 by inhibiting the protein kinase C (PKC) pathway, however, are still unknown. In the present study, there was an initial investigation of the direct effects of LPXRFa and Kiss2 on relative abundance of pituitary hormone mRNA transcripts using a whole pituitary culture system. Results indicated that LPXRFa-1 specifically functioned to increase relative abundance of lhβ mRNA when there were comparisons with the control, without any effect on relative abundance of gh, gthα and fshβ mRNA. Treatment with LPXRFa-2 resulted in a reduction in relative abundance of gthα and lhβ mRNA, and did not alter relative abundance of fshβ mRNA. Treatment of LPXRFa-2 resulted in a greater relative abundance of gh mRNA. Treatment with Kiss2, however, resulted in an increase in relative abundance of gthα and fshβ mRNA transcripts, without altering relative abundances of gh and lhβ mRNA. Subsequently, there was valuation of the potential interaction between LPXRFa and kisspeptin in COS-7 cells transfected with the cognate receptors. Both LPXRFa-1 and LPXRFa-2 suppressed serum responsive element-dependent luciferase (SRE-luc) activity when compared to stimulation with Kiss2 alone, indicating an inhibitory effect of LPXRFa on kisspeptin activation on the PKC pathway. Overall, data from the present study provide novel evidence for differential actions of LPXRFa and kisspeptin on pituitary hormone synthesis as well as for the interaction between LPXRFa and kisspeptin systems in teleosts.
       
  • Morphology, morphometry, ultrastructure, and mitochondrial activity of
           jaguar (Panthera onca) sperm
    • Abstract: Publication date: April 2019Source: Animal Reproduction Science, Volume 203Author(s): Herlon Victor Rodrigues Silva, Thalles Gothardo Pereira Nunes, Leandro Rodrigues Ribeiro, Luana Azevedo de Freitas, Moacir Franco de Oliveira, Antônio Chaves de Assis Neto, Alexandre Rodrigues Silva, Lúcia Daniel Machado da Silva The jaguar is categorized as "Near Threatened". Conservation strategies, therefore, are needed which include use of reproductive biotechniques. For implementation of biotechnique use, the reproductive characteristics of the species must be understood, which is currently not the case. This study, therefore, aimed to describe the detailed morphology of jaguar sperm, and to evaluate the sperm mitochondrial activity. Five male adults were used. Slides stained with Rose Bengal were used for morphometric and morphological analyses. The length and the width of the sperm head were measured, as well as the length of the middle piece, the tail, and the total length. Scanning and transmission electron microscopy were used for ultrastructural analysis. Mitochondrial function was assessed using the marker 3,3′-diaminobenzidine (DAB). The results are expressed as means ± SEM. The most significant morphological abnormalities observed were head (9 ± 1.7%) and tail defects (12.5 ± 3.3%). The width and length of the head were 3.6 ± 0.03 μm and 4.9 ± 0.02 μm, respectively. The middle piece measured 9.7 ± 0.3 μm, the tail measured 54.5 ± 4.4 μm, and the total length of the sperm was 59.5 ± 0.1 μm. Electron-lucent regions and approximately 54 mitochondrial spirals in the middle piece were identified in the nucleus using electron microscopy. The greatest percentages of cells were classified as DAB I (46.6 ± 4.9%) and DAB II (38 ± 4.4%). The data provide detailed information on the sperm characteristics of jaguars and can support research on germplasm conservation for the species.
       
  • Reproduction of endangered river lamprey (Lampetra fluviatilis)
           in controlled conditions
    • Abstract: Publication date: April 2019Source: Animal Reproduction Science, Volume 203Author(s): Roman Kujawa, Beata Irena Cejko, Dorota Fopp-Bayat, Sylwia Judycka, Katarzyna Glińska-Lewczuk, Cristina Maria Timofte, Joanna Nowosad, Dariusz Kucharczyk The research reported focuses on reproduction of the river lamprey Lampetra fluviatilis,(Linnaeus, 1758) in controlled conditions. There was specific emphasis on fish harvesting dates (autumn and spring), holding conditions and reproduction in a controlled environment. Attempts were also made to synchronize the time of ovulation among river lampreys, egg and sperm collections. Hormonal stimulation was conducted using carp pituitary homogenate (CPH) at a total dose of 4 mg/kg which allowed for shortening of the egg-laying period from 2 to 3 weeks to a few days while sustaining embryo survival rates and larvae quality. River lamprey males were found to not require hormonal treatment to yield good-quality sperm, as measured using the CASA system. River lamprey broodstocks adapted well to different manipulations in hatchery conditions when harvested in the autumn and spring. The results of the present study may be used to restore endangered natural populations of the river lamprey (egg and sperm collection, fertilization or gamete preservation) because ovulation and spermiation synchronization is very difficult to achieve without hormonal treatment in controlled conditions.
       
  • Serum and seminal plasma IGF-1 associations with semen variables and
           effect of IGF-1 supplementation on semen freezing capacity in buffalo
           bulls
    • Abstract: Publication date: Available online 20 March 2019Source: Animal Reproduction ScienceAuthor(s): Pradeep Kumar, Suman, Shikha Pawaria, Jasmer Dalal, Sonam Bhardwaj, S. Patil, A. Jerome, R.K. SharmaABSTRACTThe objective of the study was to establish correlation of seminal and serum IGF-1 with seminal attributes, estimate antioxidant potential of IGF-1 by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays and to study the effect of IGF-1 supplementation on semen cryopreservation. For this study, buffalo bulls were divided into sub-fertile (n = 2) and normal (n = 5) on the basis of sperm mass motility and individual motility. The serum IGF-1 concentration of normal bulls was greater than in sub-fertile bulls, but there was no difference in the seminal IGF-1 concentration among the groups. The values from correlation analyses indicated that serum IGF-1 concentration is positively correlated with semen mass motility and sperm concentration. In the second experiment, IGF-1 did not have antioxidant activities when assessed with DPPH and FRAP assays. In the third experiment, the ejaculates of normal and sub-fertile bulls were cryopreserved using semen extender in which there was IGF-1 supplementation at 0 (control), 50, 100, 150, 200, 250, 350 and 450 ng/mL of extender. Supplementation of IGF-1 at 250 ng/ml resulted in improved sperm motility, longevity and membrane intactness as compared to control after cryopreservation of semen from normal buffalo bulls, but not sub-fertile bulls. In summary, serum IGF-1 concentration was correlated with sperm mass motility and concentration in buffalo bulls and supplementation of IGF-1 protected sperm during the cryopreservation process but effects were not due to direct antioxidant activity.
       
  • Evaluation of Serum C-reactive protein concentration as a marker of
           impending parturition and correlation with progesterone profile in
           peri-partum bitches
    • Abstract: Publication date: Available online 19 March 2019Source: Animal Reproduction ScienceAuthor(s): Ada Rota, Chiara Milani, Barbara Contiero, Elisa Artusi, Bodil Ström Holst, Stefano Romagnoli C-reactive protein (CRP) is one of the major acute phase proteins in dogs. It is produced by the liver and rapidly increases in response to an inflammatory stimulus. The aim of this study was to measure CRP concentrations around parturition and to verify whether this protein could be useful, together with progesterone (P), to detect the onset of parturition in bitches. The CRP and P concentrations were measured in 66 serum samples from 28 healthy pregnant bitches, collected between -5 and +2 days from parturition (day of parturition = day 0). The effect of ‘days from parturition’, parity, and litter size on P and CRP concentration was analyzed and the correlation between CRP and P values was calculated. The P and CRP values were affected by ‘days from parturition’. While P decreased during the last days of pregnancy, CRP concentration was greater than the normal range (0- 1.07 mg/dl) starting the parturition day with the increase starting on day -1. The CRP concentration profiles during the days around parturition have not been previously reported in dogs. The increase in CRP very near the time of parturition and the low magnitude of the increase do not allow for it to be useful in clinical practice to assess the onset of parturition in the bitch.
       
  • Use of ultrasonography to determine sex in sexually immature European
           river lamprey Lampetra fluviatilis (L.)
    • Abstract: Publication date: Available online 19 March 2019Source: Animal Reproduction ScienceAuthor(s): Roman Kujawa, Joanna Nowosad, Mateusz Biegaj, Beata Irena Cejko, Dariusz Kucharczyk In this study, there was the first attempt to sex immature European river lampreys Lampetra fluviatilis, classified as Agnatha using ultrasonography. This species starts a spawning migration from seas to rivers in the autumn and reproduction is initiated in the late spring. It is recommended to collect breeders soon after the beginning of the spawning migration, however, to date no method has been developed for distinguishing the sex of individuals during this developmental period. The lampreys for the present study were caught in autumn (November) in the Vistula River (northern Poland) during the period of spawning migration and transported to the laboratory. The lampreys were anaesthetised (MS–222, dose: 0.1 g/dm3 prior to sex determinations (n = 100) using ultrasonography. The images obtained using ultrasonography were verified with post–mortem and histological examinations. The findings with this study confirmed that the sex of the European river lamprey can be effectively ascertained much earlier than can occur with assessment of external secondary sexual characteristics (e.g., sexual papilla, ovipositor, skinfold). The advantages of the method include: 100% effectiveness, survival of the fish after examination, non–invasiveness, rapid verification of the lamprey sex and the possibility of determining extent of gonadal development.
       
  • Sperm characterization of the endangered Amazonian fish Hypancistrus
           zebra: Basic knowledge for reproduction and conservation strategies
    • Abstract: Publication date: Available online 19 March 2019Source: Animal Reproduction ScienceAuthor(s): Jôsie S. Caldas, Leandro Godoy Hypancistrus zebra is an ornamental fish endemic to the Xingu River (Brazilian Amazon) and is critically endangered by the construction of a hydroelectric plant in its habitat and illegal fishing. In an attempt to create a germplasm bank for conservation purposes, in the present study there was characterization of H. zebra sperm for the first time and assessment of sperm quality throughout the year after successive stripping. Semen was collected four times during a year, and there was similar (P >  0.05) high quality for all values of sperm variables evaluated. Hypancistrus zebra sperm had an average motility rate of 88.60 ± 2.49% and membrane integrity rate of 87.93 ± 1.88%. There was a peculiar characteristic for the species, with an intermediate sperm vigor (3.00 ± 0.13) and a long duration of motility (14.72 ± 1.31 min) which is uncommon for freshwater fish. Semen had an overall mean of 79.13 ± 9.78% normal spermatozoa and 20.96 ± 9.76% of sperm cells with some morphological abnormalities. The most frequent morphological abnormalities were a degenerated head, an isolated head and a coiled flagellum. The collection of good quality semen throughout the year allows for the possible use of artificial reproduction techniques and cryopreservation for development of a germplasm bank that could contribute to successful conservation of this endangered Amazonian fish.
       
  • Comparisons in geese of the courtship, mating behaviors and fertility of
           the Carlos and Sichuan breeds and the breed crosses
    • Abstract: Publication date: Available online 15 March 2019Source: Animal Reproduction ScienceAuthor(s): Y. Zhang, Y. Yao, M.M. Wang, Y.Z. Yang, T.T. Gu, Z.F. Cao, L. Lu, C. An, J.W. Wang, G.H. Chen, Q. Xu, W.M. Zhao The Chinese goose originated from the swan goose (Anser cygnoides) and the European goose originated from the greylag goose (Anser anser). The Chinese and European geese have the potential to crossbreed. Whether interspecific differences in mating behaviors affect successful hybridization is unknown. In this study, 10-month-old Carlos geese (n = 120; Anser anser) and Sichuan geese (Anser cygnoides) were selected, and 12 multi-male parent families (3♂+12♀) were established. The courtship and mating behaviors of pure and cross-bred combinations of the Carlos and Sichuan geese were recorded using video cameras. Initiative courtship by males was the main type of courtship. Fixed mating, mating interference, and uncooperative mating were common in the flocks. The frequencies of some courtship and mating behaviors were less in the cross-bred groups (Carlos ganders × Sichuan geese, Sichuan ganders × Carlos geese) compared with the Sichuan pure-bred groups (P < 0.05). The Carlos male geese had some unique mating behaviors (i.e., one-to-one mating, formation of distinct hierarchies, and competition interference). The fertility rate had a significant correlation with the frequency of successful mating (rp = 0.992, P < 0.05), rather than with the courtship behavior. These results indicate there were lesser frequencies of courtship and successful matings in the cross-breeding than purebreeding groups. Furthermore, the fertility rate depended largely on the successful mating behavior and was independent of the courtship behavior.
       
  • Effect of dietary supplementation of palm kernel cake on ovarian and
           hepatic function in buffalo (Bubalus bubalis)
    • Abstract: Publication date: Available online 14 March 2019Source: Animal Reproduction ScienceAuthor(s): Benjamim de Souza Nahúm, Naiara Zoccal Saraiva, Cristian Faturi, André Guimarães Maciel e Silva, José de Brito Lourenço Junior, José Silva de Sousa, João Maria do Amaral Júnior, Guilherme de Paula Nogueira, Gisele Zoccal Mingoti To determine the optimal inclusion amount of palm kernel cake (PKC) in a buffalo diet, in the present study there was evaluation of the ovarian activity, metabolism and hepatic function of females that were treated to synchronize the time of ovulation. Twenty-four estrous-cyclic and non-lactating Murrah buffalo with a mean age of 5.7 years were supplemented with 0%, 0.25%, 0.5% and 1% of their body weight (BW) with PKC. Animals were subjected to the Ovsynch protocol (beginning of protocol = D0). The ovaries were examined and the blood was collected on D10 (follicular phase) and D17 (luteal phase). Follicular and luteal development and serum progesterone concentrations were not affected by diet (P > 0.05). Serum concentrations of cholesterol were greater in animals supplemented with PKC in amounts at 0.5% of BW or less with PKC, regardless of the phase of the estrous cycles when evaluations occurred (P  0.05) during the follicular and luteal phases. Triglyceride concentrations increased linearly (P = 0.03) as percentage of PKC inclusion diets increased during the follicular phase, but were similar in the luteal phase (60.0 mg/dL; P = 0.51). Amount of PKC supplementation did not affect the concentrations of alanine aminotransferase, but there was a greater amount of aspartate aminotransferase (AST) and gamma glutamyl transferase (GGT) during both phases of the estrous cycle (P 
       
  • A preliminary study of the heterogeneity in endometrial morphology and
           glycosylation in the uterine horns of the non-pregnant impala (Aepyceros
           melampus)
    • Abstract: Publication date: Available online 9 March 2019Source: Animal Reproduction ScienceAuthor(s): Carolyn J.P. Jones, W.R. (Twink) Allen, Sandra Wilsher Placentation commences only in the right uterine horn in impala (Aepyceros melampus). To investigate possible differences in morphology or glycosylation between the two horns, right and left uterine horns from six non-pregnant, wild impala were examined morphometrically and histochemically using a panel of 23 lectins and an avidin-biotin revealing system. The presence of ovarian 3ß hydroxysteroid dehydrogenase (3ßHSD) and aromatase was also investigated using immunocytochemistry. There were few detectable differences in morphology and glycosylation between right and left uterine horns in five of the specimens, but the sixth had deep clefts and plentiful exocrine secretions in the right horn, and not the left. Heavily glycosylated clusters of supranuclear granules were present in the epithelial cells, which had many classes of O-linked glycans. The serum progestagen was not markedly different, however, from that of the other specimens. In five of the six specimens, the height of luminal epithelium was greater on the right than that on the left, and the height of the gland epithelium was also greater on the right side in four of these. The 3ßHSD and aromatase activities were present in the ovaries and were similar in impala with or without progestagen concentrations>1 ng/ml in peripheral blood. No corpus haemorrhagicum or corpus luteum could be discerned. These findings indicate there are morphological and biochemical differences between right and left uterine horns in the impala and further studies are needed on both impala and other species in which placentation commences only in one uterine horn, to establish the cyclical hormone changes which induce them.
       
  • Ovarian response is not affected by the stage of seasonal anestrus or
           breed of goats when using a progesterone injection plus human chorionic
           gonadotropin-based protocol
    • Abstract: Publication date: Available online 7 March 2019Source: Animal Reproduction ScienceAuthor(s): Alan S. Alvarado-Espino, Alejo Menchaca, Cesar A. Meza-Herrera, Dalia I. Carrillo-Moreno, Santiago Zúñiga-García, Fernando Arellano-Rodríguez, Miguel Mellado, Francisco G. Véliz The aim of this study was to evaluate the effect of the stage of seasonal anestrus and breed on ovarian response in non-estrous cycling goats using a progesterone (P4) injection plus human chorionic gonadotropin (hCG)-based protocol. In Experiment 1, non-estrous cycling local Mexican goats were treated with 20 mg of P4 plus 100 IU of hCG injections 24 h apart during April (early anestrus, n = 13) or June (late anestrus, n = 12). The estrous response, interval from hCG-to-estrus, and interval to ovulation were not affected by season (P> 0.05). In addition, the size of the follicle from which ovulation occurred and the size of the corpus luteum were not different between the two stages of seasonal anestrus (P> 0.05). In Experiment 2, the estrous response was compared between multiparous non-estrous cycling local Mexican (n = 18) and Alpine (n = 19) goats in which stage of the estrous cycle was synchronized using the same P4+hCG protocol as in Experiment 1. Neither the onset of estrus nor the time of ovulation differed between breeds, and the estrus-to-ovulation interval also was similar for both breeds (P> 0.05). The diameter of the dominant follicle at the time of ovulation was similar between local and Alpine goats (P> 0.05). In addition, the pregnancy rate was not different for both local and Alpine goats (P > 0.05). In conclusion, results of this study indicate that the stage of seasonal anestrus or breed do not modify estrous and ovarian response in non-estrous cyclic goats synchronized with a P4 injection plus hCG-based protocol.
       
  • Optimization of donkey sperm vitrification: Effect of sucrose, sperm
           concentration, volume and package (0.25 and 0.5 mL straws)
    • Abstract: Publication date: Available online 5 March 2019Source: Animal Reproduction ScienceAuthor(s): M. Diaz-Jimenez, J. Dorado, C. Consuegra, I. Ortiz, B. Pereira, J.J. Carrasco, V. Gomez-Arrones, A. Domingo, M. Hidalgo The aim of this study was to assess the effect of different factors affecting vitrification success of donkey sperm: extender, sperm concentration, volume and storage vessel type. In Experiment 1, sucrose supplementations at 0.25 and 0.1 M were compared using two base extenders (containing or not egg-yolk); in Experiment 2, three sperm concentrations were assessed: 100, 200 or 300 million sperm/mL; and in Experiment 3, three different sperm volumes (100, 160 and 200 μL) and two different storage vessels (0.25 and 0.5 mL straws) were assessed. Sperm motility variables (CASA), plasma membrane and acrosome (evaluated under fluorescence microscopy) and sperm DNA integrity (flow cytometry) were evaluated after warming with comparisons of protocols. There was a greater total (55.7 ± 16.4%) and progressive (44.0 ± 11.5%) motility using the extender with egg-yolk and 0.1 M sucrose. There were no effects of sperm concentrations on vitrification results (P > 0.05). The 0.25 mL covered straw showed higher values than the 0.5 mL straw for total (50.0 ± 17.3% vs 2.0 ± 6.7%) and progressive (40.5 ± 14.9% vs 0.9 ± 1.5%) motility, plasma membrane (43.9 ± 14.4% vs 14.0 ± 16.4%) and acrosome integrity (51.5 ± 13.6% vs 28.0 ± 14.7%), respectively. In conclusion, values for donkey sperm quality variables after vitrification were greater using an extender containing egg-yolk and 0.1 M sucrose, at 300 million sperm/mL in 0.25 mL straws with outer covers.
       
  • Cellular distribution of aquaporins in testes of normal and cryptorchid
           dogs: A preliminary study on dynamic roles
    • Abstract: Publication date: Available online 5 March 2019Source: Animal Reproduction ScienceAuthor(s): A. Pelagalli, C. Squillacioti, S. Ali’, G. Liguori, N. Mirabella Fluid regulation within the male gonad is an important process for promoting sperm differentiation and maturation. Aquaporins (AQPs) are a family of thirteen integral membrane proteins involved in these processes. The expression of several genes of AQPs occurs in the male reproductive tract of humans and other animal species, although there are few studies on domestic animals. In this study, the localization of AQP7, AQP8, and AQP9 as well as the abundances of protein and mRNA transcripts were examined in normal and cryptorchid dog testes. There was immunohistochemical localization of AQP7, AQP8, and AQP9 in both the tubular and interstitial compartments of the normal and retained testes and crytorchid dogs, albeit there was an obvious difference in cellular localization with the testes from the cryptorchid dogs. These results were supported by western blotting and real-time RT-PCR analyses, there was a lesser AQP7 and greater AQP9 abundance of protein and mRNA transcripts in the cryptorchid testis. These findings indicate combined testicular functions of AQPs in cell volume regulation. In addition, with the cryptorchid condition characterized there was a different cellular distribution of AQPs supporting the thought that early detection is important for controlling possible side effects of cyptorchidism, such as pre-neoplastic and carcinogenic outcomes.
       
  • Absence of a corpus luteum and relatively lesser concentrations of
           progesterone during the period of pre-ovulatory follicle emergence results
           in lesser pregnancy rates in Bos indicus cattle
    • Abstract: Publication date: Available online 5 March 2019Source: Animal Reproduction ScienceAuthor(s): John Cavalieri Concentrations of progesterone before AI have had variable effects on fertility in both Bos indicus and Bos taurus cattle. The aim of this study was to determine if fertility and concentrations of progesterone after AI were affected in Bos indicus females when pre-ovulatory follicles develop in the absence or presence of a corpus luteum (CL). Between 6.5–7.5 days after a synchronised oestrus, all follicles ≥4 mm in diameter were aspirated (Day 0) and cloprostenol was administered on Days 0 and 1 (LP4, n = 36) or on Days 4 and 5 (HP4, n = 40). Animals were inseminated on detection of oestrus until Day 9. Breeding continued using natural mating between Days 9 and 19, AI on detection of oestrus between Day 19 and 29 and natural mating between Days 29–90. Mean concentrations of progesterone were less on Days 2–4 in the LP4 compared to the HP4-treated animals but similar on Days 14 and 20. In the LP4- compared to the HP4-treated animals, the odds of being detected in oestrus and ovulating close to the first AI were similar, but odds of pregnancy to first AI (OR = 0.19, 95% CI 0.07 – 0.52) and after 1, 4 and 13 weeks of breeding were less (P ≤  0.051). Absence of a CL and relatively lesser concentrations of progesterone during emergence of pre-ovulatory follicles resulted in lesser pregnancy rates to AI in Bos indicus cattle but did not affect concentrations of P4 after ovulation.
       
  • Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane
           lipid composition related to cold storage and cryopreservation
    • Abstract: Publication date: Available online 5 March 2019Source: Animal Reproduction ScienceAuthor(s): Rommy Díaz, Manuel Lee-Estevez, John Quiñones, Kelly Dumorné, Stefania Short, Patricio Ulloa-Rodríguez, Ivan Valdebenito, Néstor Sepúlveda, Jorge G. Farías The cold storage and cryopreservation of semen decrease sperm quality. Morphological and biochemical analyses of spermatozoa provide valuable information for the optimization of storage protocols to obtain a sufficient number of spermatozoa for in vitro fertilization. The aim of this study was to evaluate the morphology and lipid composition of Atlantic salmon (Salmo salar) spermatozoa after storage at 4 °C and cryopreservation. Semen samples were obtained by stripping. One aliquot was stored at 4 °C for 7 days, and another aliquot was cryopreserved. The morphology and ultrastructure were analysed using electron microscopy. The lipid composition was analysed by gas chromatography and a commercial kit. After cold storage, the mitochondrion was the most affected component; however, plasma membrane rupture and detachment of the flagellum were also observed. Morphological abnormalities were greater in cryopreserved spermatozoa. The head and mid-piece were dehydrated, sperm membranes were vesiculated, and alterations of mitochondria were observed. After cold storage and cryopreservation, there were less polyunsaturated and omega-3 fatty acids. Furthermore, there was an increase in saturated fatty acids and decrease in cholesterol concentration after cryopreservation (P 
       
  • Concentrations of melatonin, thyroxine, 17β-estradiol and
           11-ketotestosterone in round goby (Neogobius melanostomus) in different
           phases of the reproductive cycle
    • Abstract: Publication date: Available online 2 March 2019Source: Animal Reproduction ScienceAuthor(s): Tatiana Guellard, Hanna Kalamarz-Kubiak, Ewa Kulczykowska The aim of this study was to determine changes in concentrations of melatonin (Mel) and thyroxine (T4) in plasma, and 17β-estradiol (E2) and 11-ketotestosterone (11-KT) in plasma and gonads of female and male round gobies (Neogobius melanostomus) from the Southern Baltic Sea in four phases of the reproductive cycle classified as pre-spawning, spawning, late spawning and non-spawning periods. The concentrations of Mel, T4 and E2 were determined by radioimmunoassay (RIA) whereas 11-KT was quantified using an enzyme immunoassay (EIA). The maturity stage of gonads was determined using histological analyses. The pattern of changes in Mel concentrations of females and males was similar with the greatest concentrations in the spawning and non-spawning phases. In both sexes, there was a similar tendency of change in concentrations of T4 and E2 with the increase being in the pre-spawning and non-spawning phases. The greatest concentrations of 11-KT were observed in the plasma and gonads of males during the spawning phase. In females, there were no changes in 11-KT concentrations either in plasma or gonads during all phases where quantifications occurred. This is the first study for determination of the pattern of changes in Mel and T4 concentrations as well as gonadal steroids E2 and 11-KT, supported by histological analysis of gonads, in batch spawning fish during the reproductive cycle.
       
  • Corrigendum to “Freeze-dried spermatozoa: An alternative biobanking
           
    • Abstract: Publication date: March 2019Source: Animal Reproduction Science, Volume 202Author(s): Debora Agata Anzalone, Luca Palazzese, Domenico Iuso, Giuseppe Martino, Pasqualino Loi
       
  • Effect of plasma progesterone on oocyte recovery, oocyte quality, and
           early in-vitro developmental competence of embryos in Bos indicus dairy
           cows
    • Abstract: Publication date: March 2019Source: Animal Reproduction Science, Volume 202Author(s): Muhammad Saad, Zaeem Sarwar, Muhammad Saleem, Usman Arshad, Muhammad Shahzad, Muhammad Hassan Mushtaq, Ali Husnain, Amjad Riaz, Nasim Ahmad The objective of present study was to determine the effect of plasma progesterone (P4) on oocyte recovery, oocyte quality, and early in-vitro developmental competence of embryos in Bos indicus dairy cows. The ovaries were collected in an abattoir. These ovaries (n = 750) were divided into two groups: 1) estrous CYCLIC (n = 318), and 2) estrous ACYCLIC (n = 432). Mean serum concentrations of P4 in a subset of (n = 85; 4.21 ± 0.4 ng/ml compared with 0.5 ± 0.2 ng/ml; P 
       
  • Effect of number of 6-mm predeviation follicles and intraovarian patterns
           on right-side ovulation in heifers
    • Abstract: Publication date: March 2019Source: Animal Reproduction Science, Volume 202Author(s): O.J. Ginther, S.V. Dangudubiyyam The mechanisms underlying the greater frequency of ovulation from right ovary (RO) than left ovary (LO) were studied in 145 Bos taurus heifers. Diameter deviation during a follicular wave is indicated by continued diameter increase in the future preovulatory follicle (PF) and a decrease in diameter of future subordinate follicles. The PF (ovulation) was in the RO (63/105, 60%) more frequently (P 
       
  • Sperm motility of the Nile tilapia (Oreochromis niloticus): Effects of
           temperature on the swimming characteristics
    • Abstract: Publication date: March 2019Source: Animal Reproduction Science, Volume 202Author(s): Borys Dzyuba, Marc Legendre, Jean François Baroiller, Jacky Cosson Results of previous studies with different fish species, mostly from temperate- or cold-water habitats, indicate a species-specific diversity regarding the relationship between environmental temperature and values for sperm motility variables. In the current study, there was appraisal of environmental temperature effects on sperm motility of tilapia Oreochromis niloticus, a tropical fish species selected because of its aquaculture importance and capacity to reproduce in a broad range of water temperatures. Effects of environmental temperature on the spermatozoa motility characteristics were studied by temperature-controlled video-microscopy and CASA analysis at temperature range from 5 to 50 °C. It appeared that the Nile tilapia spermatozoa exhibit an unexpected capacity to express very different velocity characteristics over this temperature range. In the lower temperature range (5–10 °C), the percentage of motile cells was markedly variable among males. An abrupt increase in the linearity index was observed between 15 and 20 °C suggesting a physiological threshold in sperm movement at about 20 °C which is the minimum temperature for reproduction in the Nile tilapia. With faster spermatozoa velocity, there was a reduction of the motility duration at the greater temperatures. Initially, there is an increase in sperm velocity as the temperature increased until the maximal velocity occurred at 40 to 50 °C which is a temperature beyond that which occurs in natural spawning conditions. Results of the present study clearly indicate the importance of considering ambient temperature when charactering sperm motility and in determining optimal temperature conditions for fertilization in fish.
       
  • Photoperiod-treated bucks are equal to melatonin-treated bucks for
           inducing reproductive behaviour and physiological functions via the
           “male effect” in Mediterranean goats
    • Abstract: Publication date: March 2019Source: Animal Reproduction Science, Volume 202Author(s): L.A. Zarazaga, M.C. Gatica, H. Hernández, P. Chemineau, J.A. Delgadillo, J.L. Guzmán The aim of this study was to examine whether photoperiod-treated bucks have the same capacity as melatonin-treated bucks to induce reproductive responses in female goats during the spring. On 10 April, 38 anoestrous does were placed with: 1) photoperiod-treated bucks (additional light hours for 83 days from the end of the previous November; PHOTO; n = 12); 2) bucks treated with exogenous melatonin at the beginning of March (MEL; n = 13); and 3) bucks that received no treatments (CONTROL; n = 13). The bucks' sexual behaviour was assessed for 10 days, and doe oestrous behaviour was recorded for the next 32 days by checking for harness marks. Ovulation was confirmed from plasma progesterone concentration (measured twice per week) and ovulation rate was assessed by transrectal ultrasonography. Fecundity, fertility, prolificacy and productivity were also determined. The percentage of does in the PHOTO, MEL and CONTROL group: 1) having ovulations was 92%, 100% and 38% respectively; 2) expressing behavioural oestrous associated with ovulation was 92%, 100% and 31%; and 3) that became pregnant was 75%, 69% and 23%, respectively. The kids produced per doe were 1.08 ± 0.23, 1.15 ± 0.25 and 0.31 ± 0.17 for the PHOTO, MEL, and CONTROL groups, respectively with there being no differences between the PHOTO and MEL groups, however, there was a difference (P 
       
  • Effect of pre-maturation with C-type natriuretic peptide and
           3-isobutyl-1-methylxanthine on cumulus-oocyte communication and oocyte
           developmental competence in cattle
    • Abstract: Publication date: March 2019Source: Animal Reproduction Science, Volume 202Author(s): Sandra Soto-Heras, Maria-Teresa Paramio, Jeremy G. Thompson In vitro embryo production depends on oocyte competence, which is acquired during folliculogenesis, involving cytoplasmic and nuclear processes. In vitro maturation (IVM) induces spontaneous resumption of meiosis, preventing full competence acquisition. The incorporation of a pre-IVM phase with supplementation with C-type natriuretic peptide (CNP) and 3-Isobutyl-1-methylxanthine (IBMX) was used with the aim of improving developmental competence of cattle oocytes. In a preliminary experiment, COCs were cultured with increasing CNP concentrations and nuclear stage assessment was performed. Supplementation with both 100 and 200 nM CNP resulted in more germinal vesicle (GV) arrest at 6 h of culture than those in the control group (79.3%, 76.4% and 59.2%, respectively). In a second experiment, use of 100 nM CNP plus 500 μM IBMX resulted in retention of more oocytes in the GV stage (92.0%) at 6 h of culture compared to supplementation with either CNP or IBMX alone (74.8% and 86.7%, respectively). A subsequent assessment of the effect of the pre-IVM system (6-h of culture with CNP plus IBMX), followed by 20-h of IVM, with comparison to the control at 24-h of IVM was performed. Blastocyst development rate was greater after the pre-IVM phase (45.1% compared with 34.5%). The inclusion of the pre-IVM phase also resulted in an enhanced mitochondrial activity in matured oocytes and sustained integrity of transzonal projections for longer after IVM. In conclusion, CNP and IBMX function synergistically to arrest meiosis in cattle oocytes during a pre-IVM phase, which improves cumulus-oocyte communication and embryo development.
       
  • Ovulation and fertility response to commercially available GnRH products
           in lactating cows synchronized with the Double-Ovsynch protocol
    • Abstract: Publication date: March 2019Source: Animal Reproduction Science, Volume 202Author(s): M. Luchterhand, C.A. Gamarra, R.S. Gennari, P.D. Carvalho, R.V. Barletta, A.H. Souza This study was designed to evaluate whether commonly used gonadorelin products that are commercially available in the United States results in comparable ovulation and pregnancy per AI (P/AI) in synchronized lactating dairy cows. A total of 1411 Holstein cows receiving a Double-Ovsynch protocol (DOV) for conducting the first postpartum AI were randomized to receive one of the following GnRH products throughout the Double-Ovsynch: 1) Cystorelin® (CYS, gonadorelin diacetate tetrahydrate, n = 484); 2) Factrel® (FAC, gonadorelin hydrochloride, n = 482) or; 3) Fertagyl® (FER, gonadorelin diacetate tetrahydrate, n = 515). A subgroup of cows (n = 487) received ovarian ultrasound exams and collection of blood samples for progesterone (P4) analysis. Proportion of cows ovulating following the 3rd GnRH of DOV tended (P = 0.07) to differ between GnRH salts (hydrochloride = 61.5% vs. diacetate = 72.7%) but was similar for GnRH products (FER = 74.1% vs. FAC = 61.5% vs. CYS = 72.2%). Interestingly, a logistic regression analyses that considered the circulating P4 at the time of GnRH treatment indicated lower ovulation responses to FAC compared to FER and CYS; although greater circulating P4 decreased ovulation response to all GnRH products. Results for P/AI at 60 d post-insemination differed between GnRH salts (P =  0.02) as well as GnRH products (FER = 47.8% vs. FAC = 42.0% vs. CYS = 49.8%; P = 0.04). In conclusion, fertility following use of the Double-Ovsynch was less following a hydrochloride-based GnRH product likely due to lesser ovulatory responses throughout the synchronization protocol.
       
  • Subfertility effects of turmeric (Curcuma longa) on reproductive
           performance of Pseudotropheus acei
    • Abstract: Publication date: March 2019Source: Animal Reproduction Science, Volume 202Author(s): Seval Bahadir Koca, Omer Ongun, Ozlem Ozmen, Nalan Ozgur Yigit This study was conducted to determine the effect of turmeric powder dietary supplementation on the histology of visceral organs and reproductive performance of Pseudotropheus acei. The fish were fed dietary additions of 0%, 1%, 3%, 5%, and 7% turmeric powder. Each treatment was replicated three times. There were one male and four female fish with a mean weight of 6 g in each replicate. The fish were fed ad libitum for 90 days. There were no differences in fertility rate, fecundity rate, hatching rate, egg diameter, and larvae survival rate among groups (P < 0.05). Ovulation frequency and the percent of spawning broodstock were less in the 7% and 5% turmeric-supplemented group (P < 0.05), while there were no effects of treatment in the 1%, and 3% groups compared with the control group. Observations in histopathological examinations indicated there were normal tissue structures in the control, 1% and 3% turmeric-supplemented groups, while the addition of 5% and 7% turmeric induced a degeneration of and decrease in number of observable ovarian follicles. In addition, there was a normal liver structure in the control and the 1% and 3% turmeric-supplemented groups and slight to severe lipidosis in the 5% and 7% turmeric-supplemented groups. Also, the supplementation of larger amounts of turmeric induced enteritis and the slight to severe side effects on the relevant organs. These results indicate that supplementing of diets with turmeric powder in amounts of 5% and 7% suppressed ovarian follicle development, and extended periods between times of spawning which resulted in subfertility of broodstocks.
       
  • Fatty acid profile of milk for determining reproductive status in
           lactating Holstein Friesian cows
    • Abstract: Publication date: March 2019Source: Animal Reproduction Science, Volume 202Author(s): Hawar M. Zebari, S. Mark Rutter, Emma C.L. Bleach Large percentages of dairy cows do not express behavioural signs of oestrus. Faecal and urine fatty acid concentrations increase during oestrus. The objective of the present study was to determine the milk FA profile of dairy cows during the oestrous and dioestrous periods and the relationship with behavioural signs on the day of oestrus. The activity of 32 Holstein Friesian cows was measured continuously using GEA Rescounter ll pedometers (GEA Farm Technologies, Düsseldorf, Germany) and IceQubes (IceRobotics Ltd., Edinburgh, UK). Milk samples were collected on the day of oestrus and on day 14 of the subsequent oestrous cycle and analysed for FA concentration using gas chromatography (GC) and milk composition was also determined. All cows were artificially inseminated within 12 h of the onset of oestrus. On the day of oestrus, the concentration of acetic acid (P 
       
  • Out-of-season artificial reproduction of common dace (Leuciscus leuciscus
           L.) under controlled conditions
    • Abstract: Publication date: March 2019Source: Animal Reproduction Science, Volume 202Author(s): Dariusz Kucharczyk, Joanna Nowosad, Daria Joanna Kucharczyk, Krzysztof Kupren, Katarzyna Targońska, Elżbieta Wyszomirska, Roman Kujawa This study focused on the artificial reproduction of common dace Leuciscus leuciscus (L.) outside the reproductive season. The results indicate there is the possibility of initiating the reproducion in individuals of this before the natural spawning season. There could be induction of spermiation by altering the environmental conditions. For females, hormonal injections were necessary for induction of final oocyte maturation and ovulation. Generally, there were high percentages of spermiation (100%) and ovulation (87%–100%) as well as low mortality (0%–11% and 7%–13% for males and females, respectively) among the induced spawners. The greatest embryo survival occurred when Ovaprim (84.4%) or hCG (89.6%) was administered, although the latency time using hCG was at least twice as long compared to when other spawning agents were used (84–92 hrs and 30–44 hrs). The results from present study could be applicable for optimization of breeding stock numbers in aquaculture enterprises and in providing insights for conservation of L. leuciscus endangered populations.
       
  • Reproductive seasonality of male dromedary camels
    • Abstract: Publication date: March 2019Source: Animal Reproduction Science, Volume 202Author(s): Samir Al-Bulushi, B.M. Manjunatha, S.P. de Graaf, J.P. Rickard Reproductive seasonality has been reported in numerous species, including male dromedary camels, yet investigations into seasonal changes in camel semen quality have yet to be conducted. The aim of this study was to characterise the seasonal changes in camel semen quantity and quality as well as correlate these changes to testis and accessory sex gland morphology, sexual behaviour, libido and environmental factors such as day length and ambient temperature in Oman. Semen was collected twice a month for a year and testicular and accessory sex organ biometry recorded once a month via ultrasonography (n = 8 bulls). Blood samples were collected monthly to assess testosterone levels. Results indicated that testes and accessory sex glands size increased during October-April, peaking with testosterone concentrations during January (P
       
  • Use of microfluidics to sort stallion sperm for intracytoplasmic sperm
           injection
    • Abstract: Publication date: March 2019Source: Animal Reproduction Science, Volume 202Author(s): Raul A. Gonzalez-Castro, Elaine M. Carnevale We determined if microfluidic sorting (MF) of frozen-thawed stallion sperm improves sperm population characteristics and results in embryo development after intracytoplasmic sperm injection (ICSI). The efficiency and efficacy of MF sperm separation was evaluated by comparing pre- and post-separation sperm population variables. Procedural comparisons were performed after sorting with MF, single-layer colloidal centrifugation (SLC) or swim-up (SU), and cleavage and embryo development were evaluated after ICSI using MF-sorted sperm. In Experiment 1, when compared to the original sperm sample, MF sorting resulted in a sperm subpopulation with greater motility, morphology, viability, and membrane as well as DNA integrity. After sorting by MF, SLC and SU in Experiment 2, motility, viability, and membrane integrity were similar for sperm sorted using MF and SLC; however, morphology and DNA integrity were greater in sperm sorted using MF when compared with SLC. Swim-up was the least effective sorting method. In Experiment 3, sperm were processed using MF and SLC prior to ICSI. Motility, morphology and DNA integrity were similar for sperm subpopulations sorted using either method; but viability was greater for sperm sorted using MF than SLC. Sorting did not improve sperm membrane integrity. Sorting with MF prior to ICSI resulted in similar cleavage and blastocyst development rates as SLC. We concluded that MF separation of stallion sperm resulted in a subpopulation with improved sperm population parameters, comparable or better than SLC and SU. Embryos were produced after ICSI using MF sperm sorting.
       
  • Ovarian follicular and luteal characteristics in Bos indicus-influenced
           beef cows using prostaglandin F2α with or without GnRH at the onset of
           the 5-day CO-Synch + controlled internal drug release (CIDR) protocol
    • Abstract: Publication date: Available online 26 February 2019Source: Animal Reproduction ScienceAuthor(s): J.O. Scarpa, M.M. O’Neil, R.C. Cardoso, R.L Stanko, G.L. Williams A modification of the standard 5-day CO-Synch + CIDR procedure (5-day Bee Synch + CIDR; Bee Synch), developed for use in Bos indicus-influenced cows, utilizes the addition of prostaglandin F2α (PGF) on Day 0 of the protocol to eliminate mature corpora lutea (CL) and fixed-time AI (FTAI) at 66 h. Objectives were to test the hypothesis that elimination of GnRH on Day 0 (GnRH-1) does not impact significantly the synchronized development of a dominant follicle for presumptive FTAI. Seventy-one estrous cycling Brangus and Brahman x Hereford suckled cows were used in two replicates (35–36/replicate). Following stratification, cows were assigned randomly to a 2 × 3 factorial arrangement of treatments involving two truncated (no FTAI or GnRH-2) versions of Bee Synch (Bee Synch It and IIt), each begun 3, 7, and 10 days post-ovulation. Cows in Bee Synch It received 100 μg GnRH (GnRH-1), 25 mg PGF, and a CIDR on Day 0, whereas cows assigned to Bee Synch IIt received the same treatment but without GnRH-1. All cows received 50 mg PGF on Day 5 at CIDR removal. Synchronized new follicular wave emergence (NFWE; days 1–4) was observed in 68.6 and 38.9% of Bee Synch It and IIt, respectively (P =  0.01). This increased to 93.3% and 72.2%, respectively, if days 0–4 were considered. Inclusion of GnRH at CIDR insertion improved synchronized NFWE but size of the largest follicle at 66 h, the normal time of FTAI, did not differ due to treatment or day of the estrous cycle.
       
  • In vitro production of sex preselected cattle embryos using a monoclonal
           antibody raised against bull sperm epitopes
    • Abstract: Publication date: Available online 16 November 2018Source: Animal Reproduction ScienceAuthor(s): M.M.R. Chowdhury, Lianguang Xu, Rami Kong, Bun-Young Park, Ayman Mesalam, Myeong-Don Joo, Fahmida Afrin, Jong-In Jin, Hyun-Tae Lim, Il-Keun Kong Sex preselection has always generated great interest among livestock producers. Among the prevalent sperm sorting methods, there is much evidence that sex sorting has a negative effect on sperm quality with an altered pattern of sperm motility, ultimately reducing the period of cell viability. In this study, we have established a new approach for the preselected embryo production by using WholeMom®; a monoclonal antibody developed against bull sperm epitopes for simple and easy separation of X- and Y-sperm. There were no significant differences (P >  0.05) in the percentage of presumptive zygotes between the control and the X-sperm sorted group, but there was a difference in early cleaving embryos with there being 81.2 ± 1.4%, 78.3 ± 1.0%, and 66.7 ± 1.1% for the control, X-sperm sorted, and Y-sperm sorted groups, respectively. Similarly, the percentage of embryos that developed to the blastocyst stage (Day 7) were also greater (P 
       
 
 
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