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Showing 1 - 200 of 3043 Journals sorted alphabetically
AASRI Procedia     Open Access   (Followers: 15)
Academic Pediatrics     Hybrid Journal   (Followers: 20, SJR: 1.402, h-index: 51)
Academic Radiology     Hybrid Journal   (Followers: 18, SJR: 1.008, h-index: 75)
Accident Analysis & Prevention     Partially Free   (Followers: 83, SJR: 1.109, h-index: 94)
Accounting Forum     Hybrid Journal   (Followers: 23, SJR: 0.612, h-index: 27)
Accounting, Organizations and Society     Hybrid Journal   (Followers: 27, SJR: 2.515, h-index: 90)
Achievements in the Life Sciences     Open Access   (Followers: 4)
Acta Anaesthesiologica Taiwanica     Open Access   (Followers: 5, SJR: 0.338, h-index: 19)
Acta Astronautica     Hybrid Journal   (Followers: 332, SJR: 0.726, h-index: 43)
Acta Automatica Sinica     Full-text available via subscription   (Followers: 3)
Acta Biomaterialia     Hybrid Journal   (Followers: 25, SJR: 2.02, h-index: 104)
Acta Colombiana de Cuidado Intensivo     Full-text available via subscription   (Followers: 1)
Acta de Investigación Psicológica     Open Access   (Followers: 2)
Acta Ecologica Sinica     Open Access   (Followers: 8, SJR: 0.172, h-index: 29)
Acta Haematologica Polonica     Free   (SJR: 0.123, h-index: 8)
Acta Histochemica     Hybrid Journal   (Followers: 3, SJR: 0.604, h-index: 38)
Acta Materialia     Hybrid Journal   (Followers: 211, SJR: 3.683, h-index: 202)
Acta Mathematica Scientia     Full-text available via subscription   (Followers: 5, SJR: 0.615, h-index: 21)
Acta Mechanica Solida Sinica     Full-text available via subscription   (Followers: 9, SJR: 0.442, h-index: 21)
Acta Oecologica     Hybrid Journal   (Followers: 9, SJR: 0.915, h-index: 53)
Acta Otorrinolaringologica (English Edition)     Full-text available via subscription   (Followers: 1)
Acta Otorrinolaringológica Española     Full-text available via subscription   (Followers: 3, SJR: 0.311, h-index: 16)
Acta Pharmaceutica Sinica B     Open Access   (Followers: 2)
Acta Poética     Open Access   (Followers: 4)
Acta Psychologica     Hybrid Journal   (Followers: 23, SJR: 1.365, h-index: 73)
Acta Sociológica     Open Access  
Acta Tropica     Hybrid Journal   (Followers: 6, SJR: 1.059, h-index: 77)
Acta Urológica Portuguesa     Open Access  
Actas Dermo-Sifiliograficas     Full-text available via subscription   (Followers: 4)
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Actualites Pharmaceutiques Hospitalieres     Full-text available via subscription   (Followers: 4, SJR: 0.112, h-index: 2)
Acupuncture and Related Therapies     Hybrid Journal   (Followers: 3)
Ad Hoc Networks     Hybrid Journal   (Followers: 11, SJR: 0.967, h-index: 57)
Addictive Behaviors     Hybrid Journal   (Followers: 15, SJR: 1.514, h-index: 92)
Addictive Behaviors Reports     Open Access   (Followers: 5)
Additive Manufacturing     Hybrid Journal   (Followers: 8, SJR: 1.039, h-index: 5)
Additives for Polymers     Full-text available via subscription   (Followers: 20)
Advanced Drug Delivery Reviews     Hybrid Journal   (Followers: 129, SJR: 5.2, h-index: 222)
Advanced Engineering Informatics     Hybrid Journal   (Followers: 11, SJR: 1.265, h-index: 53)
Advanced Powder Technology     Hybrid Journal   (Followers: 16, SJR: 0.739, h-index: 33)
Advances in Accounting     Hybrid Journal   (Followers: 9, SJR: 0.299, h-index: 15)
Advances in Agronomy     Full-text available via subscription   (Followers: 15, SJR: 2.071, h-index: 82)
Advances in Anesthesia     Full-text available via subscription   (Followers: 25, SJR: 0.169, h-index: 4)
Advances in Antiviral Drug Design     Full-text available via subscription   (Followers: 3)
Advances in Applied Mathematics     Full-text available via subscription   (Followers: 6, SJR: 1.054, h-index: 35)
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Advances In Atomic, Molecular, and Optical Physics     Full-text available via subscription   (Followers: 16, SJR: 3.31, h-index: 42)
Advances in Biological Regulation     Hybrid Journal   (Followers: 4, SJR: 2.277, h-index: 43)
Advances in Botanical Research     Full-text available via subscription   (Followers: 3, SJR: 0.619, h-index: 48)
Advances in Cancer Research     Full-text available via subscription   (Followers: 25, SJR: 2.215, h-index: 78)
Advances in Carbohydrate Chemistry and Biochemistry     Full-text available via subscription   (Followers: 9, SJR: 0.9, h-index: 30)
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Advances in Cellular and Molecular Biology of Membranes and Organelles     Full-text available via subscription   (Followers: 12)
Advances in Chemical Engineering     Full-text available via subscription   (Followers: 24, SJR: 0.183, h-index: 23)
Advances in Child Development and Behavior     Full-text available via subscription   (Followers: 10, SJR: 0.665, h-index: 29)
Advances in Chronic Kidney Disease     Full-text available via subscription   (Followers: 10, SJR: 1.268, h-index: 45)
Advances in Clinical Chemistry     Full-text available via subscription   (Followers: 28, SJR: 0.938, h-index: 33)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 18, SJR: 2.314, h-index: 130)
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Advances in Developmental Biology     Full-text available via subscription   (Followers: 11)
Advances in Digestive Medicine     Open Access   (Followers: 4)
Advances in DNA Sequence-Specific Agents     Full-text available via subscription   (Followers: 5)
Advances in Drug Research     Full-text available via subscription   (Followers: 22)
Advances in Ecological Research     Full-text available via subscription   (Followers: 41, SJR: 3.25, h-index: 43)
Advances in Engineering Software     Hybrid Journal   (Followers: 25, SJR: 0.486, h-index: 10)
Advances in Experimental Biology     Full-text available via subscription   (Followers: 7)
Advances in Experimental Social Psychology     Full-text available via subscription   (Followers: 40, SJR: 5.465, h-index: 64)
Advances in Exploration Geophysics     Full-text available via subscription   (Followers: 3)
Advances in Fluorine Science     Full-text available via subscription   (Followers: 8)
Advances in Food and Nutrition Research     Full-text available via subscription   (Followers: 47, SJR: 0.674, h-index: 38)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 15)
Advances in Genetics     Full-text available via subscription   (Followers: 15, SJR: 2.558, h-index: 54)
Advances in Genome Biology     Full-text available via subscription   (Followers: 11)
Advances in Geophysics     Full-text available via subscription   (Followers: 6, SJR: 2.325, h-index: 20)
Advances in Heat Transfer     Full-text available via subscription   (Followers: 21, SJR: 0.906, h-index: 24)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 8, SJR: 0.497, h-index: 31)
Advances in Human Factors/Ergonomics     Full-text available via subscription   (Followers: 25)
Advances in Imaging and Electron Physics     Full-text available via subscription   (Followers: 2, SJR: 0.396, h-index: 27)
Advances in Immunology     Full-text available via subscription   (Followers: 35, SJR: 4.152, h-index: 85)
Advances in Inorganic Chemistry     Full-text available via subscription   (Followers: 9, SJR: 1.132, h-index: 42)
Advances in Insect Physiology     Full-text available via subscription   (Followers: 3, SJR: 1.274, h-index: 27)
Advances in Integrative Medicine     Hybrid Journal   (Followers: 5)
Advances in Intl. Accounting     Full-text available via subscription   (Followers: 4)
Advances in Life Course Research     Hybrid Journal   (Followers: 8, SJR: 0.764, h-index: 15)
Advances in Lipobiology     Full-text available via subscription   (Followers: 2)
Advances in Magnetic and Optical Resonance     Full-text available via subscription   (Followers: 9)
Advances in Marine Biology     Full-text available via subscription   (Followers: 16, SJR: 1.645, h-index: 45)
Advances in Mathematics     Full-text available via subscription   (Followers: 10, SJR: 3.261, h-index: 65)
Advances in Medical Sciences     Hybrid Journal   (Followers: 6, SJR: 0.489, h-index: 25)
Advances in Medicinal Chemistry     Full-text available via subscription   (Followers: 5)
Advances in Microbial Physiology     Full-text available via subscription   (Followers: 4, SJR: 1.44, h-index: 51)
Advances in Molecular and Cell Biology     Full-text available via subscription   (Followers: 22)
Advances in Molecular and Cellular Endocrinology     Full-text available via subscription   (Followers: 10)
Advances in Molecular Toxicology     Full-text available via subscription   (Followers: 7, SJR: 0.324, h-index: 8)
Advances in Nanoporous Materials     Full-text available via subscription   (Followers: 4)
Advances in Oncobiology     Full-text available via subscription   (Followers: 3)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 15, SJR: 2.885, h-index: 45)
Advances in Parallel Computing     Full-text available via subscription   (Followers: 7, SJR: 0.148, h-index: 11)
Advances in Parasitology     Full-text available via subscription   (Followers: 7, SJR: 2.37, h-index: 73)
Advances in Pediatrics     Full-text available via subscription   (Followers: 24, SJR: 0.4, h-index: 28)
Advances in Pharmaceutical Sciences     Full-text available via subscription   (Followers: 13)
Advances in Pharmacology     Full-text available via subscription   (Followers: 15, SJR: 1.718, h-index: 58)
Advances in Physical Organic Chemistry     Full-text available via subscription   (Followers: 7, SJR: 0.384, h-index: 26)
Advances in Phytomedicine     Full-text available via subscription  
Advances in Planar Lipid Bilayers and Liposomes     Full-text available via subscription   (Followers: 3, SJR: 0.248, h-index: 11)
Advances in Plant Biochemistry and Molecular Biology     Full-text available via subscription   (Followers: 8)
Advances in Plant Pathology     Full-text available via subscription   (Followers: 5)
Advances in Porous Media     Full-text available via subscription   (Followers: 4)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 18)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 19, SJR: 1.5, h-index: 62)
Advances in Psychology     Full-text available via subscription   (Followers: 60)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 5, SJR: 0.478, h-index: 32)
Advances in Radiation Oncology     Open Access  
Advances in Small Animal Medicine and Surgery     Hybrid Journal   (Followers: 2, SJR: 0.1, h-index: 2)
Advances in Space Research     Full-text available via subscription   (Followers: 345, SJR: 0.606, h-index: 65)
Advances in Structural Biology     Full-text available via subscription   (Followers: 8)
Advances in Surgery     Full-text available via subscription   (Followers: 7, SJR: 0.823, h-index: 27)
Advances in the Study of Behavior     Full-text available via subscription   (Followers: 30, SJR: 1.321, h-index: 56)
Advances in Veterinary Medicine     Full-text available via subscription   (Followers: 15)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 13)
Advances in Virus Research     Full-text available via subscription   (Followers: 5, SJR: 1.878, h-index: 68)
Advances in Water Resources     Hybrid Journal   (Followers: 43, SJR: 2.408, h-index: 94)
Aeolian Research     Hybrid Journal   (Followers: 5, SJR: 0.973, h-index: 22)
Aerospace Science and Technology     Hybrid Journal   (Followers: 309, SJR: 0.816, h-index: 49)
AEU - Intl. J. of Electronics and Communications     Hybrid Journal   (Followers: 8, SJR: 0.318, h-index: 36)
African J. of Emergency Medicine     Open Access   (Followers: 5, SJR: 0.344, h-index: 6)
Ageing Research Reviews     Hybrid Journal   (Followers: 8, SJR: 3.289, h-index: 78)
Aggression and Violent Behavior     Hybrid Journal   (Followers: 405, SJR: 1.385, h-index: 72)
Agri Gene     Hybrid Journal  
Agricultural and Forest Meteorology     Hybrid Journal   (Followers: 15, SJR: 2.18, h-index: 116)
Agricultural Systems     Hybrid Journal   (Followers: 30, SJR: 1.275, h-index: 74)
Agricultural Water Management     Hybrid Journal   (Followers: 38, SJR: 1.546, h-index: 79)
Agriculture and Agricultural Science Procedia     Open Access  
Agriculture and Natural Resources     Open Access   (Followers: 1)
Agriculture, Ecosystems & Environment     Hybrid Journal   (Followers: 53, SJR: 1.879, h-index: 120)
Ain Shams Engineering J.     Open Access   (Followers: 5, SJR: 0.434, h-index: 14)
Air Medical J.     Hybrid Journal   (Followers: 5, SJR: 0.234, h-index: 18)
AKCE Intl. J. of Graphs and Combinatorics     Open Access   (SJR: 0.285, h-index: 3)
Alcohol     Hybrid Journal   (Followers: 9, SJR: 0.922, h-index: 66)
Alcoholism and Drug Addiction     Open Access   (Followers: 6)
Alergologia Polska : Polish J. of Allergology     Full-text available via subscription   (Followers: 1)
Alexandria Engineering J.     Open Access   (Followers: 1, SJR: 0.436, h-index: 12)
Alexandria J. of Medicine     Open Access  
Algal Research     Partially Free   (Followers: 8, SJR: 2.05, h-index: 20)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 3)
Allergologia et Immunopathologia     Full-text available via subscription   (Followers: 1, SJR: 0.46, h-index: 29)
Allergology Intl.     Open Access   (Followers: 4, SJR: 0.776, h-index: 35)
ALTER - European J. of Disability Research / Revue Européenne de Recherche sur le Handicap     Full-text available via subscription   (Followers: 7, SJR: 0.158, h-index: 9)
Alzheimer's & Dementia     Hybrid Journal   (Followers: 48, SJR: 4.289, h-index: 64)
Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring     Open Access   (Followers: 5)
Alzheimer's & Dementia: Translational Research & Clinical Interventions     Open Access   (Followers: 3)
American Heart J.     Hybrid Journal   (Followers: 48, SJR: 3.157, h-index: 153)
American J. of Cardiology     Hybrid Journal   (Followers: 45, SJR: 2.063, h-index: 186)
American J. of Emergency Medicine     Hybrid Journal   (Followers: 38, SJR: 0.574, h-index: 65)
American J. of Geriatric Pharmacotherapy     Full-text available via subscription   (Followers: 6, SJR: 1.091, h-index: 45)
American J. of Geriatric Psychiatry     Hybrid Journal   (Followers: 16, SJR: 1.653, h-index: 93)
American J. of Human Genetics     Hybrid Journal   (Followers: 31, SJR: 8.769, h-index: 256)
American J. of Infection Control     Hybrid Journal   (Followers: 24, SJR: 1.259, h-index: 81)
American J. of Kidney Diseases     Hybrid Journal   (Followers: 33, SJR: 2.313, h-index: 172)
American J. of Medicine     Hybrid Journal   (Followers: 46, SJR: 2.023, h-index: 189)
American J. of Medicine Supplements     Full-text available via subscription   (Followers: 3)
American J. of Obstetrics and Gynecology     Hybrid Journal   (Followers: 191, SJR: 2.255, h-index: 171)
American J. of Ophthalmology     Hybrid Journal   (Followers: 54, SJR: 2.803, h-index: 148)
American J. of Ophthalmology Case Reports     Open Access   (Followers: 3)
American J. of Orthodontics and Dentofacial Orthopedics     Full-text available via subscription   (Followers: 6, SJR: 1.249, h-index: 88)
American J. of Otolaryngology     Hybrid Journal   (Followers: 23, SJR: 0.59, h-index: 45)
American J. of Pathology     Hybrid Journal   (Followers: 26, SJR: 2.653, h-index: 228)
American J. of Preventive Medicine     Hybrid Journal   (Followers: 21, SJR: 2.764, h-index: 154)
American J. of Surgery     Hybrid Journal   (Followers: 34, SJR: 1.286, h-index: 125)
American J. of the Medical Sciences     Hybrid Journal   (Followers: 12, SJR: 0.653, h-index: 70)
Ampersand : An Intl. J. of General and Applied Linguistics     Open Access   (Followers: 5)
Anaerobe     Hybrid Journal   (Followers: 4, SJR: 1.066, h-index: 51)
Anaesthesia & Intensive Care Medicine     Full-text available via subscription   (Followers: 55, SJR: 0.124, h-index: 9)
Anaesthesia Critical Care & Pain Medicine     Full-text available via subscription   (Followers: 10)
Anales de Cirugia Vascular     Full-text available via subscription  
Anales de Pediatría     Full-text available via subscription   (Followers: 2, SJR: 0.209, h-index: 27)
Anales de Pediatría (English Edition)     Full-text available via subscription  
Anales de Pediatría Continuada     Full-text available via subscription   (SJR: 0.104, h-index: 3)
Analytic Methods in Accident Research     Hybrid Journal   (Followers: 2, SJR: 2.577, h-index: 7)
Analytica Chimica Acta     Hybrid Journal   (Followers: 38, SJR: 1.548, h-index: 152)
Analytical Biochemistry     Hybrid Journal   (Followers: 162, SJR: 0.725, h-index: 154)
Analytical Chemistry Research     Open Access   (Followers: 8, SJR: 0.18, h-index: 2)
Analytical Spectroscopy Library     Full-text available via subscription   (Followers: 11)
Anesthésie & Réanimation     Full-text available via subscription   (Followers: 1)
Anesthesiology Clinics     Full-text available via subscription   (Followers: 22, SJR: 0.421, h-index: 40)
Angiología     Full-text available via subscription   (SJR: 0.124, h-index: 9)
Angiologia e Cirurgia Vascular     Open Access  
Animal Behaviour     Hybrid Journal   (Followers: 157, SJR: 1.907, h-index: 126)
Animal Feed Science and Technology     Hybrid Journal   (Followers: 5, SJR: 1.151, h-index: 83)
Animal Reproduction Science     Hybrid Journal   (Followers: 5, SJR: 0.711, h-index: 78)
Annales d'Endocrinologie     Full-text available via subscription   (Followers: 1, SJR: 0.394, h-index: 30)
Annales d'Urologie     Full-text available via subscription  
Annales de Cardiologie et d'Angéiologie     Full-text available via subscription   (SJR: 0.177, h-index: 13)
Annales de Chirurgie de la Main et du Membre Supérieur     Full-text available via subscription  
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Annales de Chirurgie Vasculaire     Full-text available via subscription   (Followers: 1)

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Journal Cover Animal Reproduction Science
  [SJR: 0.711]   [H-I: 78]   [5 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0378-4320
   Published by Elsevier Homepage  [3043 journals]
  • Proteomic analysis of seminal plasma from locally-adapted “Curraleiro
           Pé-Duro bulls” (Bos taurus): identifying biomarkers involved in sperm
           physiology in endangered animals for conservation of biodiversity
    • Authors: E.B. Menezes; R.V. de Oliveira; M.F. van Tilburg; E.A. Barbosa; N.V. Nascimento; A.L.M.C.S. Velho; F.B. Moreno; R.A. Moreira; A.C.O. Monteiro-Moreira; G.M.C. Carvalho; A.F. Ramos; E. Memili; A.A. Moura
      Pages: 86 - 101
      Abstract: Publication date: August 2017
      Source:Animal Reproduction Science, Volume 183
      Author(s): E.B. Menezes, R.V. de Oliveira, M.F. van Tilburg, E.A. Barbosa, N.V. Nascimento, A.L.M.C.S. Velho, F.B. Moreno, R.A. Moreira, A.C.O. Monteiro-Moreira, G.M.C. Carvalho, A.F. Ramos, E. Memili, A.A. Moura
      The present study was aimed at evaluating the seminal plasma proteins and sperm parameters of Curraleiro Pé-Duro bulls. Semen was collected from 10 bulls by electroejaculation, and sperm parameters were evaluated in fresh and frozen-thawed semen. Seminal plasma proteins were analyzed by 2-D SDS-PAGE and mass spectrophotometry. Tools in computational biology were used to generate bioinformatic knowledge and evaluate gene ontology, protein–protein interactions, phylogenetic trees and multiple sequence alignments. Sperm motility in fresh and frozen-thawed semen was 78.8±1.8% and 21.2±1.6%, respectively. Pearson’s correlations were evaluated (p<0.05). Sperm motility and vigor in fresh semen were correlated with clusterin, TIMP2 and cathepsin S (r=0.64–0.71) and sperm defects were related to inhibitor of carbonic anhydrase and BSP 5 (r=0.78–0.80). Clusterin, BSP 5, alpha-enolase, creatine kinase M-type, glyceraldehyde-3-phosphate dehydrogenase, BSP 3, albumin, and 5′-nucleotidase and legumain were correlated with acrosome intact live sperm (r=0.80–0.64). Associations were detected between sperm vigor and spermadhesin 1 (r=−0.89), and between sperm defects in fresh semen and spermadhesin 1 and clusterin (r=−0.81). Sperm motility in frozen-thawed semen was associated with BSP 1, spermadhesin 1, clusterin and spermadhesin Z13 (r=0.64–0.85). The percent of motile sperm after freeze-thawing was negatively correlated (r=−0.64) with the amount of spermadhesin 1 in the seminal plasma. Based on in silico analysis, TIMP2 interacted with BSP1, BSP3, BSP5 and metalloproteinases. Molecular functions of proteins associated with sperm parameters were binding, catalytic activity and enzymatic regulation. Amino acid sequences of spermadhesin 1 and BSP 1 from Bos taurus, and other domestic species were similar. Phylogenetic tree analysis demonstrated that clusterin from Bos taurus was related to Ovis aries and domains of clusterin, spermadhesin 1, BSP 1 and inhibitor of carbonic anhydrase were conserved as well. In summary, specific seminal proteins are associated with sperm parameters of locally-adapted bulls. Use of the endangered mammalian as a model may assist in understanding aspects of evolutionary adaptations and could improve assisted reproductive biotechnologies.

      PubDate: 2017-07-12T08:47:18Z
      DOI: 10.1016/j.anireprosci.2017.05.014
      Issue No: Vol. 183 (2017)
  • Letrozole, an aromatase inhibitor, reduces post-peak age-related
           regression of rooster reproductive performance
    • Authors: Emad Abdulgabbar Ali; Mahdi Zhandi; Armin Towhidi; Mojtaba Zaghari; Mahdi Ansari; Mojtaba Najafi; Hamid Deldar
      Pages: 110 - 117
      Abstract: Publication date: August 2017
      Source:Animal Reproduction Science, Volume 183
      Author(s): Emad Abdulgabbar Ali, Mahdi Zhandi, Armin Towhidi, Mojtaba Zaghari, Mahdi Ansari, Mojtaba Najafi, Hamid Deldar
      This study was designed to evaluate orally administrated Letrozole (Lz) on reproductive performance, plasma testosterone and estradiol concentrations and relative abundance of mRNA of GnRH, FSH and LH in roosters. Ross 308 roosters (n =32) that were 40-weeks of age were individually housed and received a basal standard diet supplemented different amounts of capsulated Lz [0 (Lz-0), 0.5 (Lz-0.5), 1 (Lz-1) or 1.5 (Lz-1.5), mg Lz/bird/day] for 12 weeks. Sperm quality variables and plasma testosterone and estradiol concentrations were assessed from the first to the tenth week of the treatment period. Semen samples from the 11th to 12th week were used for artificial insemination and eggs were collected and allotted to assess fertility and hatchability rates. Relative abundance of hypothalamic and pituitary GnRH, LH and FSH mRNA was evaluated at the end of 12th week. The results indicated that total and forward sperm motility as well as egg hatchability rate were greater in the Lz-0.5 group. Greater sperm concentrations, ejaculate volume, sperm plasma membrane integrity, testis index and fertility rates were recorded for both Lz-0.5 and Lz-1 groups compared with the Lz-0 group (P< 0.05). Body weight, percentage of sperm abnormalities, and sperm plasma membrane functionality were not affected by treatment. Testosterone and estradiol concentrations were negatively related with greater testosterone concentrations in the Lz-1.5 group which had lesser estradiol concentrations. Relative mRNA transcript abundance for GnRH, LH and FSH was Lz dose responsive being greater in the treated groups; however, this trend plateaued for GnRH and for the relative abundance of both LH and FSH mRNA was less in the Lz-1.5 group than the other treatment groups. It is concluded that Lz may be an effective treatment to improve age related post-peak reproductive performance of roosters.

      PubDate: 2017-07-12T08:47:18Z
      DOI: 10.1016/j.anireprosci.2017.05.010
      Issue No: Vol. 183 (2017)
  • Effect of light intensity on ovarian gene expression, reproductive
           performance and body weight of rabbit does
    • Authors: Liangzhan Sun; Zhenyu Wu; Fuchang Li; Lei Liu; Jinglin Li; Di Zhang; Chaoran Sun
      Pages: 118 - 125
      Abstract: Publication date: August 2017
      Source:Animal Reproduction Science, Volume 183
      Author(s): Liangzhan Sun, Zhenyu Wu, Fuchang Li, Lei Liu, Jinglin Li, Di Zhang, Chaoran Sun
      The objective of the experiment was to find the minimum light intensity which could improve reproduction by examining its effect on ovarian gene expression, reproductive performance and body weight of rabbit does with three different light intensities: 60 (L), 80 (M), and 100 (H)lx. A total of 144 Rex-rabbits submitted to a 49-day reproductive regimen were used in this study. Ovaries were collected and relative abundance of mRNA for ovarian proteins of interest was examined with real-time PCR. Amount of protein for proteins of interest was examined by immunohistochemistry. Reproductive performance and doe bodyweight of the first three consecutive reproductive periods after initiation of the light intensity treatments were evaluated. The results provided evidence that light intensity had no effect on relative abundance of estradiol receptor-α (ER-α), follicle stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), gonadotropin releasing hormone receptor 1 (GnRHR1) and progesterone receptor (PGR) mRNA. The relative abundance of growth hormone receptor (GHR) mRNA was, however, greater in Group L than M and H (P <0.05). No difference was observed for all reproductive indices as a result of submission to the three light intensities (P >0.05). The bodyweight of the does in Group L was greater than the other two groups at first insemination, second insemination and the second postpartum period (P <0.05). There was no difference in bodyweight after the second postpartum period (P >0.05). These observations suggest that light intensity between 60 and 100lx has no effect on the reproductive performance of rabbit does, however, the amounts of GHR mRNA and growth hormone (GH) protein were affected and the greater light intensity had a negative effect on bodyweight between the time of the first insemination and the second partum period.

      PubDate: 2017-07-12T08:47:18Z
      DOI: 10.1016/j.anireprosci.2017.05.009
      Issue No: Vol. 183 (2017)
  • Dietary inclusion of fish oil changes the semen lipid composition but does
           not improve the post-thaw semen quality of ram spermatozoa
    • Authors: Rommy Díaz; Mariana A. Torres; Erwin Paz; John Quiñones; Silvana Bravo; Jorge G. Farías; Néstor Sepúlveda
      Pages: 132 - 142
      Abstract: Publication date: August 2017
      Source:Animal Reproduction Science, Volume 183
      Author(s): Rommy Díaz, Mariana A. Torres, Erwin Paz, John Quiñones, Silvana Bravo, Jorge G. Farías, Néstor Sepúlveda
      The aim of this study was to investigate the effects of dietary fish oil (FO) time-response on the fatty acid profile, cholesterol levels and sperm cryosurvival in ram semen. Criollo Araucano rams were randomly assigned to two groups (n=4) according to the type of supplementation: a control group without FO and a supplemented group fed a diet with 3% FO for 8 weeks. The semen lipid profile and post-thaw sperm quality were analyzed at weeks 0 (pre-supplementation), 4, 8, 12 and 16 (post-supplementation) to evaluate the effects of FO supplementation by time interaction. Post-thaw sperm quality was determined by CASA and flow cytometry. In spermatozoa, the supplemented group increased the linoleic acid (C18:2n6c) and docosahexaenoic acid (DHA; C22:6n3) with levels higher at week 16 (P< 0.05). The effect of FO on cholesterol concentration in sperm was significant at the end of the experiment (week 16). In seminal plasma, statistical differences of butyric acid (C4:0), palmitic acid (C16:0), stearic acid (C18:0), eicosatrienoic acid (C20:3n3) and DHA were observed at week 12. The cholesterol concentration was not affected by dietary treatments (P> 0.05). However, the post-thaw sperm quality of the FO treatment group decreased. Motility percentage decreased 50% and spermatozoa with permeable plasma membrane and reacted acrosome were higher (63%) at week 16 than the control group. These results showed that DHA was effectively incorporated into semen through dietary supplementation with FO, but evaluations of post-thaw sperm quality confirm alteration specificity related to the structure of the lipid bilayer.

      PubDate: 2017-07-12T08:47:18Z
      DOI: 10.1016/j.anireprosci.2017.05.002
      Issue No: Vol. 183 (2017)
  • Characterization of male germ cell markers in canine testis
    • Authors: Won-Young Lee; Ran Lee; Hyun-Jung Park; Jeong Tae Do; Chankyu Park; Jin-Hoi Kim; Hyunjhung Jhun; Ji-Heon Lee; Taiyoung Hur; Hyuk Song
      Pages: 1 - 8
      Abstract: Publication date: July 2017
      Source:Animal Reproduction Science, Volume 182
      Author(s): Won-Young Lee, Ran Lee, Hyun-Jung Park, Jeong Tae Do, Chankyu Park, Jin-Hoi Kim, Hyunjhung Jhun, Ji-Heon Lee, Taiyoung Hur, Hyuk Song
      Spermatogenesis begins at puberty and continues throughout a male’s life. This process is initiated and maintained by spermatogonial stem cells in the seminiferous tubules, and these cells produce haploid spermatozoa. Markers of male germ cells have been fully identified in rodents, including mice and rats, but not in canines. To characterize the canine male germ cells, histological and immunohistochemical analyses were performed, using prepubertal (1–3-month-old), early pubertal (4-month-old), and postpubertal (7-month-old) dog testes. Expression of protein gene product 9.5 (PGP9.5), deleted in azoospermia-like (DAZL), synaptonemal complex protein (SCP3), tyrosine-protein kinase Kit (C-kit), and acrosin was confirmed by immunohistochemical analysis. PGP9.5 and DAZL were detected in spermatogonia and co-localized near the basement membrane of seminiferous tubules. Some SCP3-positive cells expressed PGP9.5 but not C-kit, and most of these cells were located near the basement membrane. C-kit is a marker of differentiated spermatogenic cells. In addition, acrosin was detected in C-kit-positive spematocytes and mature spermatozoa, whereas C-kit was detected in Sertoli cells in all stages of canine testis development. We suggest that male germ cell markers detected in other species are conserved in canines. PGP9.5, DAZL, SCP3, and acrosin expressions were conserved among various species, but C-kit expression varied. This study might facilitate the identification of stage-specific canine germ cell markers and cellular mechanisms of spermatogenesis.

      PubDate: 2017-06-12T04:33:08Z
      DOI: 10.1016/j.anireprosci.2017.01.002
      Issue No: Vol. 182 (2017)
  • Hedgehog-Gli1 signaling regelates differentiation of chicken (Gallus
           gallus) embryonic stem cells to male germ cells
    • Authors: Dong Li; Shaoze Cheng; Wenhui Zhang; Man Wang; Changhua Sun; Chen Zhang; Yilin Wang; Jing Jin; Yani Zhang; Bichun Li
      Pages: 9 - 20
      Abstract: Publication date: July 2017
      Source:Animal Reproduction Science, Volume 182
      Author(s): Dong Li, Shaoze Cheng, Wenhui Zhang, Man Wang, Changhua Sun, Chen Zhang, Yilin Wang, Jing Jin, Yani Zhang, Bichun Li
      Gli1 is an important signaling molecular in Hedgehog signaling pathway. In our study, we explored the adjustment effect of Hedgehog-Gli1 signaling pathway on chicken male germ cells differentiation based on the transcriptome-wide analyses of chicken ESCs, primordial germ cells (PGCs) and spermatogonia stem cells (SSCs) that were associated with male germ cell differentiation. We screened out Hedgehog signaling pathway and identified 8 candidated differentially expressed genes (DEGs), Wnt3a, Wnt16, Wnt8a, HHIPL1, Gli1, BMP6, BMP7 and TTLL4. These DEGs expression change trend among blastoderm, genital ridge and testes, from which ESCs, PGCs and SSCs were isolated was the same as RNA-Seq data with quantitative RT-PCR evaluation. Based on retinoic acid (RA) induction of ESCs to SSCs in vitro, Gli1 overexpression has the ability to induce ESCs differentiation and SSCs-like cells formation and high expression of related reproductive genes, like Cvh, C-kit, Blamp1, Prmd14, Stra8, Dazl, integrin α6 and integrin β1 and so on in vitro. While RNAi knockdown of Gli1 can protect ESCs from differentiating into SSCs and correspondingly reduce the expression of the associated reproductive gene in vivo and vitro. Immunochemistry results showed that Gli1 overexpression could increase the expression of PGCs markers Cvh and C-kit and SSCs markers integrin α6 and integrin β1 in vivo, while Gli1 knockdown can have the opposite effect in vivo and in vitro. PAS stain and flow cytometry (FCM) evaluation results indicated the quantity of germ cells is decrease or increase with Gli1 knockdown or overexpression. Collectively, these results uncovered a novel function of Gli1 and demonstrated Hedgehog-Gli1 signaling pathway involved in chicken male germ cell differentiation, where it acts as a facilitator.

      PubDate: 2017-06-12T04:33:08Z
      DOI: 10.1016/j.anireprosci.2017.02.002
      Issue No: Vol. 182 (2017)
  • Effect of different concentrations of egg yolk and virgin coconut oil in
           Tris-based extenders on chilled and frozen-thawed bull semen
    • Authors: A.A. Tarig; H. Wahid; Y. Rosnina; N. Yimer; Y.M. Goh; F.H. Baiee; A.M. Khumran; H. Salman; M. Ebrahimi
      Pages: 21 - 27
      Abstract: Publication date: July 2017
      Source:Animal Reproduction Science, Volume 182
      Author(s): A.A. Tarig, H. Wahid, Y. Rosnina, N. Yimer, Y.M. Goh, F.H. Baiee, A.M. Khumran, H. Salman, M. Ebrahimi
      The aim of this study was to evaluate the effects of 8% virgin coconut oil (VCO) combined with different percentages of egg yolk in Tris extender on the quality of chilled and frozen-thawed bull semen. A total of 24 ejaculates from four bulls were collected using an electroejaculator. Semen samples were diluted with 8% VCO in Tris extender which contained different concentrations 0% (control), 4%, 8%, 12%, 16% and 20% egg yolk. The diluted semen samples were divided into two fractions: one was chilled and stored at 4°C until evaluation after 24, 72, and 144h; the second fraction was processed by chilling for 3h at 4°C to equilibrate, then packaged in 0.25ml straws and frozen and stored in liquid nitrogen at −196°C until evaluation after 7 and 14 days. Both chilled and frozen semen samples were then thawed at 37°C and assessed for general motility using computer-assisted semen analysis (CASA), viability, acrosome integrity, and morphology (eosin–nigrosin), membrane integrity (hypo-osmotic swelling test) and lipid peroxidation (thiobarbituric acid-reactive substances (TBARS)). The results indicate treatments with 8%, 12%, 16% and 20% egg yolk with 8% VCO had greater sperm quality (P <0.05) as compared with the control. The treatment with 20% egg yolk had the greatest sperm quality (P <0.05) among the treated groups for both chilled and frozen-thawed semen. In conclusion, the use of 8% VCO combined with 20% egg yolk in a Tris-based extender enhanced the values for chilled and frozen-thawed quality variables of bull sperm.

      PubDate: 2017-06-12T04:33:08Z
      DOI: 10.1016/j.anireprosci.2017.03.024
      Issue No: Vol. 182 (2017)
  • Relationship of antral follicular blood flow velocity to superovulatory
           responses in ewes
    • Authors: M.E.F. Oliveira; P.M. Bartlewski; N. Jankowski; L.C. Padilha-Nakaghi; L.G. Oliveira; S.D. Bicudo; J.F. Fonseca; W.R.R. Vicente
      Pages: 48 - 55
      Abstract: Publication date: July 2017
      Source:Animal Reproduction Science, Volume 182
      Author(s): M.E.F. Oliveira, P.M. Bartlewski, N. Jankowski, L.C. Padilha-Nakaghi, L.G. Oliveira, S.D. Bicudo, J.F. Fonseca, W.R.R. Vicente
      The aim of this study was to examine the association between antral follicular blood flow velocity and the response of ewes to hormonal ovarian superstimulation. Ten Santa Inês ewes were subjected to a short- (7days; Group 1) or long-term (13days; Group 2) progesterone (CIDR®; InterAg, Hamilton, New Zealand) priming, and a superovulatory treatment with porcine follicle-stimulating hormone (pFSH; Folltropin®-V; Bioniche Animal Health, Belleville, ON, Canada), given twice daily for four consecutive days in decreasing doses and initiated four or ten days after CIDR insertion, respectively. Embryos were recovered surgically seven days after the last pFSH dose. From one day prior to until the end of the pFSH regimen (Days −1 to 3), all ewes underwent daily transrectal ultrasonography of ovaries. The number of high-velocity pixels (HVPs; 0.055–0.11m/s or upper 50% of recordable velocities) on Day 1 correlated directly with the number of corpora lutea (CL; r =0.92, P =0.0002) and of viable embryos (r =0.77, P =0.01). Correlations were also recorded between the number of HVPs on Day 3 and the recovery rate (r =−0.69, P =0.03), viability rate (r =−0.64, P =0.05), and percentage of degenerated embryos (r=0.65, P=0.04). The percentage of HVPs relative to the total area of ovarian cross section on Day 1 was correlated with the number of CL (r =0.95, P< 0.001) and of viable embryos (r =0.85, P =0.002). This parameter on Day 3 was also correlated with the recovery rate (r =−0.69, P =0.03). The percentage of HVPs relative to the total Doppler area on Day 0 was correlated with the recovery rate (r =0.72, P =0.02). It can be concluded that sonographic assessment of high-velocity antral follicular blood flow has the makings of a useful non-invasive method to predict the outcome of the superovulatory treatment in ewes.

      PubDate: 2017-06-12T04:33:08Z
      DOI: 10.1016/j.anireprosci.2017.04.009
      Issue No: Vol. 182 (2017)
  • Comparison of visual and computerized estrous detection and evaluation of
           influencing factors
    • Authors: Ina Gaude; Andreas Kempf; Klaas Dietrich Strüve; Martina Hoedemaker
      Abstract: Publication date: Available online 29 July 2017
      Source:Animal Reproduction Science
      Author(s): Ina Gaude, Andreas Kempf, Klaas Dietrich Strüve, Martina Hoedemaker
      The aim of this study was to compare the automatic estrous detection system (AED) Heatime® with visual estrous detection (VED). The study was conducted on 139 Holstein Friesian cows in one dairy herd in Northern Germany. The cows were fitted with activity collars from d 21 postpartum until d 40 of gestation and a 30-minute visual estrous observation was conducted three times a day. In addition, as a separate part of the VED, estrous detection by exclusive consideration of standing estrus (SE) was investigated. Ovulation detected by regular trans-rectal ultrasonography and serum progesterone analyses served as gold standard to calculate estrous detection rate (EDR) and reliability rate (RR) for each of the three estrous detection systems (AED, VED and SE). Change in body condition antepartum and postpartum, lameness, milk yield and milk fat- milk protein- ratio (FPR) on the expression and detection of estrus were investigated. Estrus was more precisely detected by the AED (EDR: 85.1%) than by VED (EDR: 52.2%) and SE (EDR: 22.3%) (P <0.05). The RR when using the three methods did not differ (P >0.05). Changes in body condition, lameness, milk yield or the FPR were not associated with the estrous detection rate by the AED. The estrous detection rate by VED in lame animals (EDR: 24.2%) was, however, less than in cows without any lameness (EDR: 52.7%; P< 0.05). In conclusion, the AED Heatime® system can be effectively used for estrous detection and can be used to more precisely detect estrus than with VED.

      PubDate: 2017-08-03T13:45:42Z
      DOI: 10.1016/j.anireprosci.2017.07.019
  • Integrity of head and tail plasmalemma is associated with different
           kinetic variables in boar sperm
    • Authors: Adéla Grieblová; Eliana Pintus; José Luis Ros-Santaella
      Abstract: Publication date: Available online 29 July 2017
      Source:Animal Reproduction Science
      Author(s): Adéla Grieblová, Eliana Pintus, José Luis Ros-Santaella
      An intact and functional sperm plasmalemma has a major role in sperm motility and fertilizing capacity. Several techniques have been developed to evaluate the integrity of the sperm plasma membrane, but there are still some inconsistencies concerning the methods that are more closely associated with sperm function. In this study, the aim was to: i) evaluate the integrity of the boar sperm plasmalemma during 72h of semen storage at 17°C using four techniques: eosin/nigrosin (E/N), propidium iodide/carboxyfluorescein diacetate (PI/CFDA), hypo-osmotic swelling test (HOST), and combined HOST with eosin staining (HOST/E), ii) assess the correlations and the limits of consistency among these techniques, iii) and estimate the relationships with the acrosomal status and sperm kinetics. Results indicate that the integrity of the sperm plasmalemma decreases during 72h of storage, although significant differences were found only using the HOST and HOST/E techniques. Moreover, use of E/N and PI/CFDA results in greater values relative to the undamaged sperm membrane than use of HOST and HOST/E at any incubation time. Overall, results using all techniques were consistent and correlate except for findings with PI/CFDA and HOST, which was slightly below 95%. Moreover, values using the techniques for the evaluation of the integrity of the sperm head and tail membranes are positively associated with the acrosomal status and different kinetic variables with the tail integrity being related to rapid linear trajectories and the head integrity to rapid curvilinear trajectories. The results of this study provide new insights into the relevance of evaluating the boar sperm plasmalemma in the routine spermiogram.

      PubDate: 2017-08-03T13:45:42Z
      DOI: 10.1016/j.anireprosci.2017.07.020
  • Fertility disturbances of dimethylacetamide and glycerol in rooster sperm
           diluents: Discrimination among effects produced pre and post
           freezing-thawing process
    • Authors: F.M.K. Abouelezz; M.A.M. Sayed; J. Santiago-Moreno
      Abstract: Publication date: Available online 29 July 2017
      Source:Animal Reproduction Science
      Author(s): F.M.K. Abouelezz, M.A.M. Sayed, J. Santiago-Moreno
      With avian sperm cryopreservation protocols, the most widely used cryoprotectants (CPAs) are the glycerol (GLY; in gradual freezing: in-straw freezing method), and the dimethylacetamide (DMA; in pellets by plunging into liquid nitrogen: in-pellet rapid freezing method). Use of both methods results in a small portion of thawed live sperm with lesser fertilizing ability compared with the semen samples immediately after collection. This study was conducted to assess the pre-freezing damage occurring to the sperm due to the interaction with the cryoprotectants (CPAs) GLY (8%) and DMA (5%), as well as the post-freezing damage resulting from both freezing methods Data for each treatment, in fresh and frozen-thawed samples, were compared for sperm motility, fertilizing capacity and sperm-egg penetration holes/germinal disc (SP holes/GD). Hens (n =50) were artificially inseminated (10 hens/treatment) six times with 3day intervals between inseminations. The treatment of fresh sperm with DMA led to a reduction (P< 0.05) in the count of SP holes/GD (21.4) and the fertility rate (66.7%). The addition and elimination of GLY in fresh samples resulted in a lesser (P< 0.05) number of SP holes/GD (11.8) and the fertility rate (i.e., 50.0%). The number of SP-holes/GD was least in frozen-thawed samples using both DMA and GLY (14.2 and 9.2, respectively). The fertility rate when using semen frozen with DMA in- pellets was greater (P< 0.05) than with use of semen that had been frozen using GLY in straws (46.4% compared with 31.3%). The reduction in fertility compared with the control when semen was cryopreserved using GLY was 64.1%; the GLY addition and elimination was responsible for two thirds of this reduction. The reduction in fertility when using semen cryopreserved with DMA was 46.7%; half of the reduction was attributed to the treatment with DMA. In conclusion, the mechanical damage attributed to the process for reducing GLY concentrations was more harmful to sperm fertilizing capacity than the toxicity of DMA and freeze/thaw process. For both freezing methods, the amount of sperm cryo-damage was similar, when the damage attributed to the CPA addition and elimination process was excluded.

      PubDate: 2017-08-03T13:45:42Z
      DOI: 10.1016/j.anireprosci.2017.07.021
  • Assessment of butorphanol-azaperone-medetomidine combination as anesthesia
           for semen collection and evaluation of semen quality in white-tailed deer
           (Odocoileus virginianus)
    • Authors: S.M. Kirschner; R. Rodenkirch
      Abstract: Publication date: Available online 25 July 2017
      Source:Animal Reproduction Science
      Author(s): S.M. Kirschner, R. Rodenkirch
      The aim of this current study was to evaluate the level of anesthesia produced by a combination of butorphanol-azaperone-medetomidine (BAM) for semen collection by electroejaculation on captive white-tailed bucks (Odocoileus virginianus). Ten male white-tailed deer, weighing 68.2–115.9kg, ranging in age from one to four years were randomly selected from housing pens and anesthetized with the BAM drug combination at a dose volume of 2.0mL each. Semen was collected from each animal using a standard cervid electroejaculation protocol while under BAM anesthesia. Physiological data was recorded following induction of anesthesia and during semen collection. Collected ejaculates were prepared for analysis using a standard extender protocol for cryopreservation. Eleven sperm viability parameters were quantified for each sample using a Computerized Assisted Sperm Analysis system, including total seminal volume; sperm concentration and total sperm number. kinematic parameters of motile spermatozoa were also assessed. Results demonstrated that BAM provided an effective plane of anesthesia for successful collection of viable sperm. Measured physiological variables of heart rate, respiration and body temperature all remained within safe, normal limits. Data recorded on semen characteristics from all collected ejaculates correlated well with key traits determined to be important for successful fertilization through measurement of total semen volume; sperm concentration; total sperm number; and kinematic parameters of motile spermatozoa. There were no serious adverse events. This field study indicates that BAM anesthesia is suitable for semen collection in white-tailed deer.

      PubDate: 2017-08-03T13:45:42Z
      DOI: 10.1016/j.anireprosci.2017.07.016
  • Effects of adding different levels of Glutamine to modified Beltsville
           extender on the survival of frozen rooster semen
    • Authors: Aytak Bakhshayesh Khiabani; Gholamali Moghaddam; Hossein Daghigh Kia
      Abstract: Publication date: Available online 25 July 2017
      Source:Animal Reproduction Science
      Author(s): Aytak Bakhshayesh Khiabani, Gholamali Moghaddam, Hossein Daghigh Kia
      The aim of the present study was to investigate the effects of l-glutamine on the quality of frozen-thawed rooster semen. Semen samples were collected from eight mature roosters (Ross 308). After initial semen assessments, samples of adequate quality were mixed together and diluted with modified Beltsville extender without l-glutamine (control) and supplemented with 2.5, 5, and 7.5mM l-glutamine. Semen straws were subjected to cryopreservation and evaluated twice at 15-day intervals. After thawing, sperm viability, total and progressive sperm motilities were measured by Eosin-Nigrosine and Computer-Aided Sperm Analysis (CASA), respectively. The results showed that sperm functions decreased on day 30 compared to day 15. The extender supplemented with 5mM glutamine improved (p<0.05) sperm viability, total and progressive sperm motilities compared to other treatments and the control group. The best level of glutamine appeared to be 2.5mM, as it provided the highest sperm membrane integrity and the lowest level of abnormalities. The results of this study suggest that the addition of glutamine to the diluent improves semen quality and using glutamine allows rooster sperm to be frozen for longer.

      PubDate: 2017-08-03T13:45:42Z
      DOI: 10.1016/j.anireprosci.2017.07.013
  • Effect of 11β-hydroxyandrostenedione on European whitefish Coregonus
           lavaretus (Linnaeus, 1758)
    • Authors: Jarosław Król; Piotr Hliwa; Adam Polewacz; Agnieszka Stabińska; Stefan Dobosz; Konrad Ocalewicz
      Abstract: Publication date: Available online 25 July 2017
      Source:Animal Reproduction Science
      Author(s): Jarosław Król, Piotr Hliwa, Adam Polewacz, Agnieszka Stabińska, Stefan Dobosz, Konrad Ocalewicz
      The goal of the present research was to evaluate the efficiency of 11β-hydroxyandrostenedione (OHA) applied in the diet to achieve sex reversal in the European whitefish (Coregonus lavaretus). At 32day post-hatching, fish were reared in four groups: fish fed with 10ppm of OHA (10 OHA), fish fed with 20ppm of OHA (20 OHA), fish fed without OHA (C) and fish fed without OHA and reared in the water from 20 OHA group (R). The experimental groups were conducted in separate recirculation systems and the first phase of the experiment lasted 63days. For the histological analysis of the gonads, fish from all groups were reared without OHA treatment for an additional 91days (second phase). At the end of the first phase of the experiment, survival of the whitefish ranged from 34.5±11.1% to 51.5±7.3%. The final body weight and coefficient of variation in the weight ranged from 5.6±1.2g to 6.9±1.5g and from 21.5 to 22.7%, respectively. No negative effects of OHA treatment on the growth and the survival of the whitefish were found. Six histological categories of the whitefish gonads were observed. Apart from the typical ovaries and testes, two types of the intersexual gonads (ovotestis and testis-ova) and two types of the sterile-altered gonads were distinguished. No gonadal females were found among fish from any of OHA groups. Gonadal males constituted of 60% and 50% of the fish from 10 OHA and 20 OHA groups, respectively. Intersexes were observed in all groups with the highest proportion found among fish from R variant. Rate of sterile individuals in 10 OHA and 20 OHA groups was 17% and 30%, respectively. The proportion of fish with normal testes to fish with other types of gonad varied from 0.43:1 to 1.5:1 with the higher ratio observed in both OHA groups. Lack of the females among fish from OHA groups suggested OHA affected growth and development of ovaries in the whitefish. However, a high percentage of the sterile fish in both OHA treated groups indicated application of lower doses of OHA for masculinization of the whitefish in the further research.

      PubDate: 2017-08-03T13:45:42Z
      DOI: 10.1016/j.anireprosci.2017.07.015
  • Hyaluronic Acid Improves Frozen-thawed Sperm Quality and Fertility
           Potential in Rooster
    • Authors: Saied Lotfi; Morteza Mehri; Mohsen Sharafi; Reza Masoudi
      Abstract: Publication date: Available online 25 July 2017
      Source:Animal Reproduction Science
      Author(s): Saied Lotfi, Morteza Mehri, Mohsen Sharafi, Reza Masoudi
      Beneficial effects of Hyaluronic acid (HA) has not been yet assessed for cryopreservation of rooster sperm. This study was conducted to evaluate the effects of different concentrations of HA (0, 1, 2, 4 and 8mM) in Beltsville extender on the cryopreservation of rooster sperm. Semen samples were collected from six Ross broiler breeders (24-week) using abdominal massage, then divided into five equal aliquots and cryopreserved in Beltsville extender that contained different concentrations of HA. Motion characteristics, morphology, membrane functionality, viability, acrosome integrity, lipid peroxidation and fertility potential of sperm were assessed after thawing. HA at concentration of 2mM (HA2) resulted in the highest (P<0.05) total motility (55.3±1.1%) and progressive motility (25.2±0.8%) compared to the other groups. HA8 produced the lowest significant (P<0.05) percentage of total (38.6±1.1%) and progressive (14.7±0.8%) motility. High significant percentage of membrane functionality were observed in HA1 and HA2 (43.2±1.0 and 46.1±1.0%, respectively) compared to HA4 (40.1±1.0%) and HA8 (32.5±1.0%). Moreover, HA1 and HA2 produced the higher percentage of acrosome integrity (54.8±1.2 and 57.5±1.2, respectively) compared to other groups. HA1 and HA2 reduced (P<0.05) malondialdehyde formation (3.66±0.08 and 3.75±0.08 nmol/ml) compared to other groups. Fertility rate and hatching rate obtained from artificial insemination were significantly higher in HA1 (63.7 and 54.7%) and HA2 (67.5 and 57.7%) compared to control group (40 and 37%). Our results showed that supplementation of Beltsville extender with 1 and 2mM HA significantly improved the quality of rooster sperm after freeze thawing.

      PubDate: 2017-08-03T13:45:42Z
      DOI: 10.1016/j.anireprosci.2017.07.018
  • Semen quality, antioxidant status and reproductive performance of rabbits
           bucks fed milk thistle seeds and rosemary leaves
    • Authors: Youssef A. Attia; Rawia S. Hamed; Fulvia Bovera; Abd El-Hamid E. Abd El-Hamid; Mohammed A. Al-Harthi; Hossam A. Shahba
      Abstract: Publication date: Available online 23 July 2017
      Source:Animal Reproduction Science
      Author(s): Youssef A. Attia, Rawia S. Hamed, Fulvia Bovera, Abd El-Hamid E. Abd El-Hamid, Mohammed A. Al-Harthi, Hossam A. Shahba
      The study aimed to investigate the effects of milk thistle seeds (MTS) and rosemary leaves (RL) both at 5 and 10g/kg diet on reproductive performance, semen quality and blood metabolites of rabbit bucks. A total of 35 rabbit bucks were randomly distributed into five experimental groups (7 bucks/group). All the groups were fed the same basal diet. The 1st group (control) did not have MTS and RL in its basal diet. The 2nd and 3rd groups were supplemented with MTS at 5 and 10g/kg, respectively. The 4th and 5th groups were fed the basal diet supplemented with RL at 5 and 10g/kg, respectively. The sperm concentration (SC), total sperm output (TSO), live sperm (LS), total live sperm (TLS) and total motile sperm (TMS) were significantly greater in the bucks fed MTS at 10 and RL at 5g/kg diet than the control group. Bucks fed MTS at 10g/kg diet had higher fertility than the control. Also, RL 5g/kg group showed higher testosterone and fertility than the control, but the MTS 10g/kg group showed the highest value for both parameters. In conclusion, MTS and RL at 10 and 5g/kg, respectively, significantly improved the semen quality and the fertility and MTS also increased the economic efficiency of rabbit bucks.

      PubDate: 2017-08-03T13:45:42Z
      DOI: 10.1016/j.anireprosci.2017.07.014
  • Apoptosis and cell proliferation in porcine placental vascularization
    • Authors: Eva G. Sanchis; Andrea L. Cristofolini; Mariana R. Fiorimanti; Claudio G. Barbeito; Cecilia I. Merkis
      Abstract: Publication date: Available online 23 July 2017
      Source:Animal Reproduction Science
      Author(s): Eva G. Sanchis, Andrea L. Cristofolini, Mariana R. Fiorimanti, Claudio G. Barbeito, Cecilia I. Merkis
      The placenta is a highly vascularized organ, indispensable tothe transfer of nutrients to the growing fetuses. During gestation, there exists an expansion of the placental vascular network through active angiogenesis. The aim of this research was to study cell proliferation and apoptosis through high resolution light microscopy (HRLM) and transmission electron microscopy (TEM) ultrastructure, immunohistochemistry for Ki67and caspase-3, determination of placental vascular area,and TUNEL assay. Crossbred sows placental tissues from approximately 30±2(n=5), 40±2(n=5), 60±2 (n=5), 80±2(n=5), 90±2(n=5) and 114±2(n=5) days of gestation were used. The evaluation of cell proliferation showed the highest%Ki67 values on days 30±2 and 80±2 of pregnancy. Caspase-3 expressed the highest value on day 30±2, while the highest apoptotic indexes were found on days30±2 and 90±2. The placental vascular area was higher on day 80±2 of pregnancy. According to our results, an active vascular cell remodeling by a caspase-3 dependent apoptosis seems to be present in early pregnancy. The increase in the vascular area on day 80±2 would be the result of the intense vascular cell proliferation detected with Ki67. Further studies are needed to understand the complex processes of angiogenesis, cell proliferation and apoptosis that interact in the placenta during porcine gestation.

      PubDate: 2017-08-03T13:45:42Z
      DOI: 10.1016/j.anireprosci.2017.06.009
  • Divergently Expressed Gene Identification and Interaction Prediction of
           Long Noncoding RNA and mRNA Involved In Duck Reproduction
    • Authors: Jindong Ren; Xue Du; Tao Zeng; Li Chen; Junda Shen; Jianhong Hu; Lizhi Lu
      Abstract: Publication date: Available online 23 July 2017
      Source:Animal Reproduction Science
      Author(s): Jindong Ren, Xue Du, Tao Zeng, Li Chen, Junda Shen, Jianhong Hu, Lizhi Lu
      Long noncoding RNAs (lncRNAs) and divergently expressed genes exist widely in different tissues of mammals and birds, in which they are involved in various biological processes. However, there is limited information on their role in the regulation of normal biological processes during differentiation, development, and reproduction in birds. In this study, whole transcriptome strand-specific RNA sequencing of the ovary from young ducks (60 days), first-laying ducks (160 days), and old ducks, i.e., ducks that stopped laying eggs (490 days) was performed. The lncRNAs and mRNAs from these ducks were systematically analyzed and identified by duck genome sequencing in the three study groups. The transcriptome from the duck ovary comprised 15,011 protein-coding genes and 2,905 lncRNAs; all the lncRNAs were identified as novel long noncoding transcripts. The comparison of transcriptome data from different study groups identified 2,240 divergent transcription genes and 135 divergently expressed lncRNAs, which differed among the groups; most of them were significantly downregulated with age. Among the divergent genes, 38 genes were related to the reproductive process and 6 genes were upregulated. Further prediction analysis revealed that 52 lncRNAs were closely correlated with divergent reproductive mRNAs. More importantly, 6 remarkable lncRNAs were correlated significantly with the conversion of the ovary in different phases. Our results aid in the understanding of the divergent transcriptome of duck ovary in different phases and the underlying mechanisms that drive the specificity of protein-coding genes and lncRNAs in duck ovary.

      PubDate: 2017-08-03T13:45:42Z
      DOI: 10.1016/j.anireprosci.2017.07.012
  • Metritis vaccination in Holstein dairy heifers using a herd-specific
           multivalent vaccine – Effects on uterine health and fertility in first
    • Authors: M. Freick; A. Kunze; O. Passarge; J. Weber; S. Geidel
      Abstract: Publication date: Available online 22 July 2017
      Source:Animal Reproduction Science
      Author(s): M. Freick, A. Kunze, O. Passarge, J. Weber, S. Geidel
      In cattle, acute puerperal metritis (APM) compromises animal welfare and has an economic impact to the farmer because of the detrimental effects on reproductive performance, milk production, and survivability. The aim of this randomized clinical study was to investigate the effects of a prepartum immunization using a herd-specific multivalent vaccine on incidence of APM, prevalence of clinical endometritis (CE), and selected measures of reproductive performance in primiparous Holstein cows. Pregnant nulliparous cows (230±4days of gestation) assigned to the vaccinated group (VG; n=142) received subcutaneously 5.0mL of a multivalent herd-specific vaccine containing inactivated whole bacterial cells of Trueperella pyogenes, Escherichia coli, Streptococcus uberis, Bacteroides species, and Peptostreptococcus species obtained from uterine swabs of primiparous cows suffering from APM at the time of enrolment and three weeks later. Heifers allocated to the control group (Co; n=144) remained unvaccinated. Rectal temperature in the first 10days in milk (DIM) did not differ between treatments, and no interaction between treatment and day was observed (interaction treatment*day: p=0.623). Incidence of APM (VG, 46.0%, Co, 48.9%, p=0.588), number of antibiotic doses per cow to treat APM (VG, 0.63±0.81, Co, 0.64±0.75, p=0.496), prevalence of CE at 35 DIM (VG, 24.6%, Co, 19.3%, p=0.350), first service pregnancy per artificial insemination (VG, 40.3%, Co, 45.5%, p=0.541), number of inseminations until 150 DIM (VG, 2.0±1.4, Co, 1.9±1.3, p=0.749), interval from calving to first service (median days, VG, 52, Co, 52, p=0.915), and interval from calving to pregnancy (median days, VG, 90, Co, 83, p=0.419) did not differ between VG and Co. Treatment did not affect activity of aspartate aminotransferase (AST) and creatine kinase (CK) or concentrations of beta-hydroxybutyrate (BHB) and haptoglobin in serum samples collected at 5 DIM. Furthermore, treatment did not influence yields of milk, fat or protein in the first 100 DIM. Moreover, no signs that vaccination provided an attenuation of severity of the APM cases were detected as the course of rectal body temperature and values of AST, CK, BHB, and haptoglobin were not influenced by treatment within the group of cows with diagnosed APM. Use of this herd-specific multivalent vaccine designed to reduce metritis was not efficacious at reducing the risk of uterine disease or influencing production and reproduction in dairy cows.

      PubDate: 2017-07-23T13:26:36Z
      DOI: 10.1016/j.anireprosci.2017.07.011
  • Effect of kisspeptin on the proliferation and apoptosis of bovine
           granulosa cells
    • Authors: Hongyu Liu; Gaoqing Xu; Zhiyu Yuan; Yangyunyi Dong; Jun Wang; Wenfa Lu
      Abstract: Publication date: Available online 20 July 2017
      Source:Animal Reproduction Science
      Author(s): Hongyu Liu, Gaoqing Xu, Zhiyu Yuan, Yangyunyi Dong, Jun Wang, Wenfa Lu
      Previous studies have shown that kisspeptin (Kp-10) is expressed in mammalian ovaries; however, the expression and role of Kp-10 in bovine ovarian granulosa cells are still unclear. In this study, we assessed the expression of Kp-10 and its effects on the proliferation and apoptosis of bovine granulosa cells. Immunohistochemical analysis showed that Kp-10 was expressed in the cytoplasm of bovine ovarian granulosa cells. Moreover, MTT assays showed that 100nM Kp-10 significantly inhibited the viability of granulosa cells (P< 0.05). Flow cytometry analysis showed that Kp-10 could significantly increase accumulation of cells in the G1 phase, decrease accumulation of cells in the S phase, and promote apoptosis in bovine granulosa cells (P< 0.05). Additionally, Kp-10 decreased the mRNA levels of Bcl-2, an anti-apoptotic gene; increased the mRNA levels of caspase-3, a pro-apoptotic gene; and increased the mRNA levels of Fas and Fasl (P<  0.05). Thus, our findings demonstrated for the first time that Kp-10 inhibited proliferation and promoted apoptosis in bovine ovarian granulosa cells. These findings provide insights into our understanding of the role of Kp-10 in mediating the proliferation of bovine granulosa cells.

      PubDate: 2017-07-23T13:26:36Z
      DOI: 10.1016/j.anireprosci.2017.07.008
  • Impact of Cryopreservation Method on Dromedary Camel Ovary Structure,
           Viability, and Development of Antral Follicular Oocytes
    • Authors: M.M. Madboly; E.S. Abdel-Aal; Eitedal H. Elsayed
      Abstract: Publication date: Available online 19 July 2017
      Source:Animal Reproduction Science
      Author(s): M.M. Madboly, E.S. Abdel-Aal, Eitedal H. Elsayed
      The objectives of this study were, a) to compare two different vitrification techniques, the solid surface vitrification (SSV) and direct vitrification (DV) method, b) to evaluate the effect of four cryoprotectant agents and their toxicity on the morphological appearance and ultrastructural of camel ovarian cortex, c) to examine the development of oocytes recovered from the vitrified ovarian cortex. Fragments of ovarian cortex were exposed to equilibration solution consisting of TCM- 199 with 10% fetal camel serum (FCS); 0.10M sucrose and including one of the following cryoprotectants; 20% glycerol (GLY); 3.5M ethylene glycol (EG); 3.5M propanediol (PROH) or 3M dimethylsulphoxide (DMSO). After vitrification of ovarian fragments, they were warmed and evaluated by histological and transmission electron microscope. The oocytes isolated from vitrified ovarian cortex were cultured in TCM-199 at 38.5∘C under 5% CO2 for 44h. Maturation was indicated through cumulus expansion and calculated by oocytes reaching first telophase and second metaphase (TI+MII). The percentage of morphologically normal and viable follicles of SSV was significantly higher P <0.05 than DV group (52.9 vs. 38.1, respectively). In conclusion, viability, histological and ultrastructural observations revealed that SSV method and ethylene glycol-based freezing solution were able to remain morphology better follicle and oocyte. Additionally, most organelles of oocytes are able to recover their normal morphology in camel ovarian cortex following cryopreservation and thawing processes, and oocytes isolated from vitrified ovarian cortex can exhibit maturation and reaches to (TI+MII).

      PubDate: 2017-07-23T13:26:36Z
      DOI: 10.1016/j.anireprosci.2017.07.006
  • Effects of dietary propolis on the number and size of pleopadal egg,
           oxidative stress and antioxidant status of freshwater crayfish (Astacus
           leptodactylus Eschscholtz)
    • Authors: Serpil Mişe Yonar; Kenan Köprücü; Muhammet Enis Yonar; Sibel Silici
      Abstract: Publication date: Available online 19 July 2017
      Source:Animal Reproduction Science
      Author(s): Serpil Mişe Yonar, Kenan Köprücü, Muhammet Enis Yonar, Sibel Silici
      Four different crayfish diets; control, E1, E2 and E3, respectively containing 0, 1, 2 and 4% propolis, were tested to determine the effects of dietary propolis on the number and size of pleopadal egg, and malondialdehyde (MDA) level, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities in the freshwater crayfish (Astacus leptodactylus). The crayfish were kept at 9.6±5.3°C water temperature and fed three times daily during a six month period The pleopodal egg number (from 7 to 9) produced per gram of the body weight and total pleopodal egg number (from 201 to 263) significantly increased (P< 0.05) with the dietary propolis supplemantation. However, an increase in the dietary propolis led to a significant decrease (P< 0.05) in the pleopodal egg size (from 3.22mm to 2.76mm). MDA level significantly (P< 0.05) decreased in the hepatopancreas (from 4.78 to 3.04 nmol/g protein) and ovarium (from 3.52 to 1.98 nmol/g protein) of the crayfish fed with the increased dietary propolis level. On the other hand, an increase in the dietary propolis led to a significant increase (P< 0.05) in SOD activities in hepatopancreas (from 21.8 to 41.1U/g protein) and ovarium (from 16.8 to 26.8U/g protein). However, CAT activities significantly decreased (P< 0.05) in the hepatopancreas (from 23.8 to 18.9 nmol/g protein) and ovarium (from 21.8 to 17.5 nmol/g protein) of the crayfish fed with the increased dietary propolis level. Similarly, an increase in the dietary propolis caused a significant decrease (P< 0.05) in GSH-Px activities in the hepatopancreas (from 21.8 to 41.1U/g protein) and ovarium (from 16.8 to 26.8U/g protein) with the formation of the pleopodal egg. The dietary propolis improves reproductive efficiency in the crayfish and decreases the oxidative stress under controlled hatchery conditions.

      PubDate: 2017-07-23T13:26:36Z
      DOI: 10.1016/j.anireprosci.2017.07.010
  • Effect of pyrethroids on female genital system. Review
    • Authors: Elena Marettova; Milan Maretta; Jaroslav Legáth
      Abstract: Publication date: Available online 16 July 2017
      Source:Animal Reproduction Science
      Author(s): Elena Marettova, Milan Maretta, Jaroslav Legáth
      Pyrethroids have been associated with a range of toxicological effects on various organs in animals.Recent animal studies suggest that neurodevelopmental, reproductive, and immunological effects may result following exposure to some pyrethroids at levels below those that induce overt signs of neurotoxicity. A variety of pyrethroids and their metabolites have the potential to affect the reproductive system. Dose-dependent effects on reproduction are associated with exposure across pyrethroid types. In mammals, permethrin and tetramethrin and cypermethrin have been found to be associated with adverse effects at high doses. Fenvalerate, deltamethrin, cypermethrin, caused morphometric and structural changes in the female genital organs. These pyrethroids affect ovulation, cause atresia of follicles, decrease the number of follicular cells, oocytes and corpora lutea and induce vesicular atrophy of the endometrial glands. The potential hormonal activity of pyrethroids showed that certain pyrethroids and their metabolites have multiple effects on the endocrine system. The level of steroid hormones, such as progesterone and estradiol, was inhibited. The pyrethorids may have the potential to mimic estrogens or to inhibit estrogen action. Some metabolites of pyrethroids, in particular permethrin and cypermethrin, are more likely to interact with the cellular estrogen receptors than the parent pyrethroids. Though several pyrethroids posses low toxicity, some pyrethroids, such as deltamethrin, cypermethrin, fenvalerate and bifenthrin have showed considerable toxicity.

      PubDate: 2017-07-23T13:26:36Z
      DOI: 10.1016/j.anireprosci.2017.07.007
  • Differential expression of subunits of 20β-hydroxysteroid dehydrogenase
           during gametogenesis in rainbow trout (Oncorhychus mykiss)
    • Authors: Arya Vazirzadeh; Yann Guiguen
      Abstract: Publication date: Available online 14 July 2017
      Source:Animal Reproduction Science
      Author(s): Arya Vazirzadeh, Yann Guiguen
      The patterns of expression of two subunits of 20β-hydroxysteroid dehydrogenase (20β-HSD), key enzyme involved in the biosynthesis and activation of steroid hormones, were examined in rainbow trout by using a combination of quantitative real-time PCR and in-situ hybridization. The expression of targeted genes was examined in mRNA extracted from different tissues at different gonadal stages in male and female trout. Both subunits of 20β-HSD were found to be widely distributed in tissues. The highest expression of 20β-HSD A was found in intestine followed by skin, stomach, liver and gills, whereas, the highest expression of 20β-HSD B was observed in stomach followed by head kidney, ovary − at late vitellogenesis stage- and trunk kidney. In ovarian tissue 20β-HSD A was highly expressed in mature oocytes, and the highest expression of 20β-HSD B was in ovary at late vitellogenesis stage. There were no differences in the level of expression of either subunit among groups of rainbow trout at different stages of maturational competence. In male fish, 20β-HSD A was highly expressed in testis stage I in contrast to 20β-HSD B which was highly expressed in testis stage VIII. In situ- hybridization results showed that the 20β-HSD gene was highly expressed in gastrointestinal organs, while only slightly expressed in the gonadal tissue of fish at stage 62day-post-fertilization (dpf). Overall, the results confirm the ubiquitous presence of 20β-HSD among tissues in rainbow trout with relatively minor fluctuations in expression associated with reproductive cycles which collectively suggests a wider metabolic role of these enzymes than just an association with the synthesis of control hormones for reproduction.

      PubDate: 2017-07-23T13:26:36Z
      DOI: 10.1016/j.anireprosci.2017.07.009
  • Spermatogenesis in a neotropical marsupial species, Philander frenatus
           (Olfers, 1818)
    • Authors: A.F.A. Figueiredo; D.A. Cordeiro; J.C. Nogueira; S.A. Talamoni; L.R. França; G.M.J. Costa
      Abstract: Publication date: Available online 13 July 2017
      Source:Animal Reproduction Science
      Author(s): A.F.A. Figueiredo, D.A. Cordeiro, J.C. Nogueira, S.A. Talamoni, L.R. França, G.M.J. Costa
      Despite the singular morphology of the male genital system and the different reproductive strategies of marsupials, little emphasis has been given to the testis morphology and spermatogenic kinetics in this mammalian order. The present study aimed to investigate the testis function and the duration of spermatogenesis in the southeastern four-eyed opossum, Philander frenatus. Testes of six adult males were routinely processed for histological and stereological analyses. In order to determine the duration of spermatogenesis, intratesticular injections of tritiated thymidine were performed 1h, 13days and 21days before the sacrifice. Based on the development of the acrosomic system, ten stages of the seminiferous epithelium cycle were characterized. The mean body and testis weights for the P. frenatus were respectively 326±20g and 0.4±0.05g, providing a gonadosomatic index of 0.3±0.02%. The most advanced germ cell types labeled at 1h, 13days and 21days after thymidine injections were, respectively, preleptotene spermatocytes at stage IV, pachytene spermatocytes at stage IV and diplotene spermatocytes at stage IX. Based on the stages frequencies and the most advanced labeled germ cells, each spermatogenic cycle and the entire spermatogenic process lasted respectively 13.5±0.5 and 60.9±2.4days. When compared to the vast majority of eutherian mammals already investigated, these data indicate that the Philander frenatus presents a relatively long duration of spermatogenesis.

      PubDate: 2017-07-23T13:26:36Z
      DOI: 10.1016/j.anireprosci.2017.07.004
  • The effect of antioxidants on sperm motility activation in the Booroolong
    • Authors: L.M. Keogh; P.G. Byrne A.J. Silla
      Abstract: Publication date: August 2017
      Source:Animal Reproduction Science, Volume 183
      Author(s): L.M. Keogh, P.G. Byrne, A.J. Silla
      Motile sperm can generate high levels of reactive oxygen species (ROS) post activation, and ROS can quickly accumulate to levels that impair motility and fertilising ability. The addition of antioxidants to sperm suspensions has been suggested as a means of reducing oxidative stress and enhancing sperm motility during and after sperm storage. Despite this, very few studies have attempted to experimentally test the effects of antioxidants on sperm motility activation in animals that use an external mode of fertilisation, espcially in amphibians. The present study aimed to investigate the effect of vitamin C and vitamin E on sperm motility activation in the Booroolong frog. Spermatozoa were activated in media containing either vitamin C (0, 0.05, 0.10, 0.15, 0.20, 0.25μgμL−1) or vitamin E (0, 0.25, 0.50, 0.75, 1.0, 1.25 1.50, 1.75μgμL−1). Sperm performance parameters (percent motility and velocity) were assessed using CASA at 0, 1, 2, 3, 4, 5 and 6h post-activation. Contrary to expectations, vitamin C supplementation was detrimental to sperm motility across all tested concentrations, while vitamin E had no effect. Further investigation on the endogenous antioxidant system of anuran sperm is required to ascertain whether alternative antioxidants may be more suitable at reducing ROS produced during sperm activation and improving sperm motility activation in vitro.

      PubDate: 2017-07-12T08:47:18Z
  • Clinical use of fetal measurements to determine the whelping day in German
           shepherd breed bitches
    • Authors: Marta Cecchetto; Chiara Milani; Juri Vencato; Hasan Sontas; Antonio Mollo; Barbara Contiero; Stefano Romagnoli
      Abstract: Publication date: Available online 12 July 2017
      Source:Animal Reproduction Science
      Author(s): Marta Cecchetto, Chiara Milani, Juri Vencato, Hasan Sontas, Antonio Mollo, Barbara Contiero, Stefano Romagnoli
      The aim of this work was to use a linear regression model previously developed in a pilot study to calculate days before parturition (DbP) using inner chorionic cavity (ICC), biparietal diameter (BPD), crown-rump length (CRL), body diameter (BD) and deep portion of telencephalic vesicle (DPTV) in German shepherd dogs (GSD) with known ovulation day and then to test that model in bitches with unknown ovulation day. In our current study, a model for GSD bitches published in a previous report, proved satisfactory for ICC [DbP=44.76−(4.34×ICC)] and BPD [DbP=38.65−(12.86×BPD)]. We therefore used their model, but developed a new one for CRL, BD and DPTV. For ICC and BPD, we tested accuracy for more than 35days before parturition (ICC) and more than 15days before parturition (BPD). Measurements were taken on at least two fetuses during each ultrasound recording (US) of 22 GSD bitches with known (n=16) and unknown (n=6) ovulation days. The accuracy of the above model was 77–100% for ICC and 83-96% for BPD with a precision of ±1 and ±2 days, respectively. Accuracy increased significantly when US was performed more than 35days before parturition for ICC and more than 15days before parturition for BPD. BD and CRL were the most accurate parameters (R2 =0.95 and 0.85). In bitches with unknown ovulation day, BD accuracy was 71.4–100% with a precision of ±1day and ±2 days, respectively. CRL and DPTV were less accurate (±1day, 60%; ±2 days, 80% accuracy).

      PubDate: 2017-07-12T08:47:18Z
      DOI: 10.1016/j.anireprosci.2017.07.005
  • Corrigendum to “Reproductive cycles of marine mammals” [Anim. Reprod.
           Sci. 124 (2011) 184–193]
    • Authors: Pomeroy
      Abstract: Publication date: Available online 10 July 2017
      Source:Animal Reproduction Science
      Author(s): P. Pomeroy

      PubDate: 2017-07-12T08:47:18Z
  • Evaluation of changes in blood flow of the uterine artery by Doppler
           ultrasonography during the estrous cycle in lactating Bos indicus cows
    • Authors: Mubbashar Hassan; Abdul Sattar; Muhammad Bilal; Muhammad Avais; Nasim Ahmad
      Abstract: Publication date: Available online 9 July 2017
      Source:Animal Reproduction Science
      Author(s): Mubbashar Hassan, Abdul Sattar, Muhammad Bilal, Muhammad Avais, Nasim Ahmad
      The objective of this study was to evaluate the changes in uterine blood flow (UBF) based on the resistance index (RI) and the pulsatility index (PI) by Doppler ultrasonography throughout the estrous cycle in lactating Sahiwal cows (n=9). Cows were randomly selected during their spontaneous estrus. UBF was examined on alternate days in all cows during the estrous cycle. The results revealed that the mean RI values were higher (P< 0.05) on day −1 (estrus) than on day 0 (ovulation). The mean RI values followed a consistent pattern on days 2, 4, 6, and 8 (metestrus to early diestrus) and were lower (P< 0.05) than on days 10, 14 16, and 18 (diestrus to early proestrus). The RI and corpus luteum (CL) size negatively correlated during its static phase (r=−0.99; P< 0.05). The mean RI was greater (P< 0.05) in high producers than in low producing cows. The mean PI value was higher (P< 0.05) on day 10 than on day −1 and day 0. Similarly, the mean PI values were lower (P < 0.05) on days 2 and 4 than on day 10. The PI value remained significantly lower (P< 0.05) on day 2 than on day 8 of the estrous cycle. There was a positive correlation between RI and P4 (r=0.70; P< 0.05) and PI and P4 (r=0.56; P< 0.05) during the estrous cycle. It is concluded that the RI of uterine arteries, as a measure of blood flow, is considerably lower, while the PI is substantially elevated during diestrus compared to estrus and ovulation in Sahiwal cows.

      PubDate: 2017-07-12T08:47:18Z
      DOI: 10.1016/j.anireprosci.2017.07.001
  • Effects of sex, pregnancy and season on insulin secretion and carbohydrate
           metabolism in horses
    • Authors: Elisabeth Beythien; Manuela Wulf; Natascha Ille; Jörg Aurich; Christine Aurich
      Abstract: Publication date: Available online 6 July 2017
      Source:Animal Reproduction Science
      Author(s): Elisabeth Beythien, Manuela Wulf, Natascha Ille, Jörg Aurich, Christine Aurich
      In pregnant mares, peripheral insulin antagonism channels glucose preferentially to the foetus. On the other hand, horses reduce their metabolic activity in winter. Taking these aspects of equine pregnancy and metabolism together, we hypothesized that glucose clearance from blood and the insulin response to glucose do not only change throughout gestation but also with season. To test this hypothesis, the glucose and insulin response to an oral glucose test and relative insulin release were analysed in pregnant mares (n=12) and in geldings (n=10) as controls. Animals were tested in June, September, December, and in March (geldings) and on day 320 of gestation (mares). Furthermore, the 6 mares foaling early and 6 foaling later in the year were compared. In mares and geldings, plasma glucose concentration increased after glucose feeding (p<0.001). The increase was more pronounced in mares (p<0.05) and increased from June to December in mares (p<0.001) but not geldings (month x group p<0.05). This indicates constant glucose clearance in geldings but reduced clearance in pregnant mares. A partial insulin resistance is thus induced by pregnancy independent from season. Insulin release increased after glucose feeding (p<0.001) similarly in geldings and mares. The insulin response to glucose and relative insuslin release increased from June to December (p<0.001) indicating seasonal changes in β-cell sensitivity. Glucose and insulin concentration did not differ between early and late foaling mares. In conclusion, in horses, β-cell sensitivity to glucose is affected by season while insulin sensitivity during pregnancy decreases independent from season.

      PubDate: 2017-07-12T08:47:18Z
      DOI: 10.1016/j.anireprosci.2017.07.002
  • Genetic variation and correlated changes in reproductive performance of a
           red tilapia line selected for improved growth over three generations
    • Authors: Ngo Phu Thoa; Azhar Hamzah; Nguyen Hong Nguyen
      Abstract: Publication date: Available online 6 July 2017
      Source:Animal Reproduction Science
      Author(s): Ngo Phu Thoa, Azhar Hamzah, Nguyen Hong Nguyen
      The present study examines genetic variation and correlated changes in reproductive performance traits in a red tilapia (Oreochromis spp.) population selected over three generations for improved growth. A total of 328 breeding females (offspring of 111 sires and 118 dams) had measurements of body weight prior to spawning (WBS), number of fry at hatching (NFH), total fry weight (TFW) and number of dead fry (NDF) or mortality of fry including unhatched eggs at hatching (MFH). Restricted maximum likelihood (REML) analysis in a multi-trait model showed that there are heritable genetic components for all traits studied. The heritability for WBS was very high (0.80). The estimates for traits related to fecundity (NFH, TFW) and survival (NDF) were low and they were associated with high standard errors. Genetic correlations of WBS with other reproductive performance traits (NFH, TFW and NDF) were generally positive. However, NFH was negatively correlated genetically with TFW. As expected, body measurements during growth stage exhibited strong positive genetic correlations with WBS. The genetic correlations between body traits and reproductive performance (NFH, TFW, NDF) were not significant. Correlated responses in reproductive traits were measured as changes in least squares means between generations or spawning years. Except for WBS that increased with the selection programs, the phenotypic changes in other reproductive traits observed were not statistically significant (P>0.05). It is concluded that the selection program for red tilapia has resulted in very little changes in reproductive performance of the animals after three generations. However, periodic monitoring of genetic changes in fecundity and fitness related traits such as NDF or MFH should be made in selective breeding programs for red tilapia.

      PubDate: 2017-07-12T08:47:18Z
      DOI: 10.1016/j.anireprosci.2017.07.003
  • GPER1 in Sand Rat Epididymis: Effects of Seasonal Variations, Castration
           and Efferent Ducts Ligation
    • Authors: Rafik Menad; Meriem Fernini; Souaâd Smaï; Xavier Bonnet; Thérèse Gernigon-Spychalowicz; Elara Moudilou; Farida Khammar; Jean-Marie Exbrayat
      Abstract: Publication date: Available online 23 June 2017
      Source:Animal Reproduction Science
      Author(s): Rafik Menad, Meriem Fernini, Souaâd Smaï, Xavier Bonnet, Thérèse Gernigon-Spychalowicz, Elara Moudilou, Farida Khammar, Jean-Marie Exbrayat
      Estrogen plays a crucial role in regulating epididymal function and development. Estrogen signaling is mediated via two main receptors essentially involved in the genomic regulating pathway: ERα and ERβ. Recent studies revealed the contribution of a novel estrogen receptor involved in the non-genomic pathway: GPER1. This receptor belongs to the family of seven-transmembrane G-protein-coupled receptors and it triggers rapid cellular responses. Immuno-histochemical studies and Western Blot analyses were performed to investigate the GPER1 expression in the caput and cauda epididymis of free-ranging fat sand rats (Psammomys obesus) captured during the breeding and resting seasons. We also investigated the effect of castration (C), castration followed by testosterone treatment (C+T), and ligation of the efferent ducts (L). During the breeding season, a marked positive GPER1 immunoreactivity was detected in the cytoplasm of principal cells and basal cells; this signal persisted during the resting season, attenuated however, meanwhile the clear cells were not immuno-reactive. In C animals, the immuno-histochemical staining underwent nuclear translocation. In C+T animals, this response became nuclear and cytoplasmic. In the L group, the expression of the GPER1 was mainly located in the cytoplasm of principal cells and in the nuclei of basal cells; the sperm was also immune-positive in the cauda epididymis. Western blot analysis showed that GPER1 has a molecular weight of 55kDa in the caput and cauda epididymis during the breeding season, and it persisted during the resting season in the caput epididymis with a decrease in the cauda epididymis. These results suggest that GPER1 mediate a specific cellular estrogen signaling with marked differences between the breeding and resting seasons. Experimental groups suggest that testosterone is involved in the regulation of the expression of GPER1, in addition to other estrogen signalization pathways.

      PubDate: 2017-07-03T05:41:36Z
      DOI: 10.1016/j.anireprosci.2017.06.012
  • Age related changes in laying pattern and egg weight of different laying
           hen genotypes
    • Abstract: Publication date: Available online 23 June 2017
      Source:Animal Reproduction Science
      Author(s): E. Tůmová, L. Uhlířová, R. Tůma, D. Chodová, L. Máchal
      The aim of the study was to evaluate changes in laying patterns depending on the age of different genotypes of laying hens. In the experiment, six genotypes were evaluated (brown-egg hens Bovans Brown, Bovans Sperwer and Isa Sussex, white-egg hens Dekalb White, and laying hens with tinted shells Moravia Barred and Moravia BSL) in three periods during the laying cycle (the onset of lay between 20 and 26 weeks of age, the middle from 36 to 42 weeks of age and the end of lay between 64 and 70 weeks of age). A significant interaction between genotype and age was apparent in mean sequence length (P<0.001), length of the prime sequence (P<0.001), mean number of sequences (P<0.001) and mean time of oviposition (P˂0.001). The longest lag during the course of the experiment was with Moravia BSL, which was more than 3h; the shortest lag was observed in Bovans Brown, which was less than 1h. The mean time of oviposition was also affected by genotype (P˂0.001). Bovans Brown laid their eggs approximately 3.5h after the lights came on, whereas Moravia BSL laid their eggs almost 6h after the lights came on. Egg weight increased with age (P˂0.001), and the smallest differences in egg weight were with ISA Sussex (5g), whereas the biggest differences were with Moravia BSL (10g).

      PubDate: 2017-07-03T05:41:36Z
  • Sperm membrane proteins associated with the boar semen cryopreservation
    • Authors: Daianny B. Guimarães; Tatyane B. Barros; Maurício F. van Tilburg; Jorge A.M. Martins; Arlindo. A. Moura; Frederico B. Moreno; Ana C. Monteiro-Moreira; Renato A. Moreira; Ricardo Toniolli
      Abstract: Publication date: Available online 20 June 2017
      Source:Animal Reproduction Science
      Author(s): Daianny B. Guimarães, Tatyane B. Barros, Maurício F. van Tilburg, Jorge A.M. Martins, Arlindo. A. Moura, Frederico B. Moreno, Ana C. Monteiro-Moreira, Renato A. Moreira, Ricardo Toniolli
      This study aimed to define sperm membrane protein markers of semen freezability of boars with the aid of a proteomic approach. Semen from fourteen adult boars were subjected to slow freezing and rapid thawing. After thawing, sperm vigor and motility were analyzed, and based on these results, animals were separated into two groups: good (GFEs) and poor freezability (PFEs). Sperm membrane proteins were extracted and subjected to two-dimensional electrophoresis. Stained gels were analyzed by computerized resources to indicate differentially expressed protein spots, that were identified by mass spectrometry. Six animals showed good freezability with average sperm vigor and motility of 2.2±0.8 and 41.8±22.9, respectively, whereas eight boars showed poor freezability, with 1.9±0.6 and 26.8±17.5 of sperm vigor sperm motility, respectively. An average of 263±62.2 spots per gel and 234.2±54.6 of spots consistently present in all gels were detected. The intensities of five spots were significantly different between groups. Fc fragment of IgG binding protein and lactadherin were more intense in the PFE group, while Arylsulfatase A and F-actin capping protein subunit alpha 1 were more expressed in the GEF group. Based on their functions and interactions with other proteins, we conclude that these four sperm membrane proteins may act as potential markers of boar semen freezability.

      PubDate: 2017-06-22T05:17:37Z
      DOI: 10.1016/j.anireprosci.2017.06.005
  • Ovum pick-up interval in buffalo (Bubalus bubalis) managed under wetland
           conditions in Argentina: effect on follicular population, oocyte recovery,
           and in vitro embryo development
    • Authors: J. Konrad; G. Clerico; M.J. Garrido; G. Taminelli; M. Yuponi; R. Yuponi; G. Crudeli; M. Sansinena
      Abstract: Publication date: Available online 20 June 2017
      Source:Animal Reproduction Science
      Author(s): J. Konrad, G. Clerico, M.J. Garrido, G. Taminelli, M. Yuponi, R. Yuponi, G. Crudeli, M. Sansinena
      The excellent adaptation of water buffalo (Bubalis bubalis) to swampy environments means that animals are frequently managed in areas with restricted access for reproductive procedures. The objective of this study was to evaluate the effect of the ovum pick-up (OPU) interval on follicular population, oocyte recovery, oocyte quality and in vitro embryo production. Twelve Murrah buffaloes were subjected to two consecutive dominant follicle reductions, and randomly assigned to either 7-day (n=6) or 14-day (n=6) OPU interval groups. Although there was no significant difference in the average number of small (<3mm) and large (>8mm) diameter follicles available per OPU, a higher proportion of medium-sized follicles (3–8mm) were observed in the 14-day interval group (5.129 vs 3.267; p<0.05). The number of recovered oocytes per donor was also significantly higher (4.51 vs. 2.8; p<0.05) in the 14-day interval group, although this was attributed to an increase in the proportion of lower quality oocytes (grades III and IV). After in vitro fertilization, embryo developmental competence from grade I and II oocytes was superior to that from grade III and IV oocytes, irrespective of OPU interval group. There was no significant difference in the proportion of grade I and II oocytes cleaved after sperm co-incubation; however, there was a higher proportion of blastocysts produced in 14-day interval group (28 vs. 6%, p<0.05). No blastocysts were produced from grade III and IV oocytes. This study indicates it is possible to use a 14-day interval for oocyte collection in water buffalo; this approach could be considered as an alternative when access to animals is restricted.

      PubDate: 2017-06-22T05:17:37Z
      DOI: 10.1016/j.anireprosci.2017.06.004
  • Protocols for sperm cryopreservation in the domestic cat: a review
    • Authors: K. Buranaamnuay
      Abstract: Publication date: Available online 13 June 2017
      Source:Animal Reproduction Science
      Author(s): K. Buranaamnuay
      The main objectives of sperm cryopreservation in domestic cats are to preserve these gametes for future use, especially in valuable domestic cat breeds and to use knowledge-gained for developing sperm preservation techniques in wild felids that are threatened with extinction. To achieve acceptable quality of post-thaw sperm and results after insemination, sperm samples must be properly handled, cryopreserved and thawed by using appropriate protocols. In this paper, cryopreservation protocols of domestic cat sperm that have been reported previously are described. The subtopics include sources of sperm, freezing extenders, methods of sperm dilution, freezing storage vessels, methods of sperm cryopreservation, thawing temperature, and thawing extenders. In addition, comparisons of sperm quality results for different treatments within the same studies and between different studies are also presented.

      PubDate: 2017-06-15T04:37:17Z
      DOI: 10.1016/j.anireprosci.2017.06.002
  • Yucca schidigera can promote rabbit growth, fecundity, affect the release
           of hormones in vivo and in vitro, induce pathological changes in liver,
           and reduce ovarian resistance to benzene
    • Authors: Martina Földešiová; Andrej Baláži; Ľubica Chrastinová; Juraj Pivko; Jan Kotwica; Abdel Halim Harrath; Peter Chrenek; Alexander V. Sirotkin
      Abstract: Publication date: Available online 10 June 2017
      Source:Animal Reproduction Science
      Author(s): Martina Földešiová, Andrej Baláži, Ľubica Chrastinová, Juraj Pivko, Jan Kotwica, Abdel Halim Harrath, Peter Chrenek, Alexander V. Sirotkin
      This study evaluated the effect of Yucca schidigera (YS) extract on the physiological, reproductive, and endocrine indexes of New Zealand White rabbit does. Six-week-old rabbit does were fed a standard diet (control group) or a diet enriched with 5 or 20g of Y powder extract per 100-kg feed mixture for 350 days. The does were artificially inseminated after induction of superovulation. Weight gain; conception and kindling rate; viability of pups and mothers; histopathological state of liver and muscle; plasma levels of progesterone (P4), oxytocin (OT), and prostaglandin F (PGF); and the release of P4, insulin-like growth factor I (IGF-I), OT, and PGF by isolated ovarian fragments and their response to the addition of benzene were analyzed. YS extract supplementation promoted weight gain and induced histopathological changes in the liver (creased vacuolization and occurrence of fuchsinophile inclusions in hepatocytes, liver fibrosis, hyperemia, occurrence of Kupffer cells, signs of necrosis and inflammation). YS consumption was not associated with changes in muscle (occurrence of fuchsinophile inclusions and signs of atrophy, interstitial edema, and inflammation), although Y2 increased muscle vascularization. YS supplementation increased conception and kindling rates but did not affect viability of pups or adult animals. Moreover, it enhanced plasma OT and PGF levels; plasma P4 concentration was increased by low-dose YS, but decreased by high-dose YS. Cultured ovarian fragments isolated from YS-fed does released more P4 and PGF and less IGF-I than ovarian fragments of control animals. However, YS supplementation did not affect ovarian OT release. Benzene alone did not influence the release of hormones by ovaries of control does. YS supplementation induced the inhibitory effect of benzene on the release of PGF, but not on other ovarian hormones. Collectively, these results suggest that dietary supplementation of YS extract can stimulate rabbit performance (growth and fecundity), which may be due to the promotion of P4, OT, and PGF release. It could, however, induce some pathological changes in the liver and reduce resistance of ovaries to the environmental contaminant benzene.

      PubDate: 2017-06-12T04:33:08Z
      DOI: 10.1016/j.anireprosci.2017.06.001
  • Multiple paternity in the cultured yellow pond turtles (Mauremys mutica)
    • Authors: Xin-cheng Zhang; Jian Zhao; Wei Li; Cheng-qing Wei; Xin-ping Zhu
      Abstract: Publication date: Available online 9 June 2017
      Source:Animal Reproduction Science
      Author(s): Xin-cheng Zhang, Jian Zhao, Wei Li, Cheng-qing Wei, Xin-ping Zhu
      As a result of hunting and habitat loss, wild populations of the yellow pond turtle, Mauremys mutica, are decreasing. The International Union for Conservation of Nature considers M. mutica to be an endangered species. All studied freshwater turtles have polyandrous mating with multiple paternity. To survey the mating strategies of M. mutica, 1year’s genetic data of parents and all offspring in an artificially captive population were analyzed. Two groups of multiplex PCR containing 16 microsatellite loci were used to analyze the paternity of 302 hatchlings from 132 parents and from 159 clutches. The genetic data indicated that multiple paternity is rare in M. mutica, occurring in only seven of 138 clutches. Although the frequency of multiple paternity was only 5.07%, results of the present research indicate that M. mutica has a polyandrous mating system. In the breeding season, the successive clutches of 34 females each had the same paternity as the previous clutches. It was observed that four males (f85, f58, f87, and f76) had more than 20 offspring each, totaling 99 and representing 32.78% of all offspring. This finding implies that paternity is competitive in this artificially captive population and might bias the genetic diversity of the offspring.

      PubDate: 2017-06-12T04:33:08Z
      DOI: 10.1016/j.anireprosci.2017.06.003
  • Heat and Chemical Treatments in Adult Cyprinus carpio (Pisces
           Cypriniformes) Rapidly Produce Sterile Gonads
    • Authors: Sullip Kumar Majhi; Avinash Rambhau Rasal; Basdeo Kushwaha; Sudhir Raizada
      Abstract: Publication date: Available online 3 June 2017
      Source:Animal Reproduction Science
      Author(s): Sullip Kumar Majhi, Avinash Rambhau Rasal, Basdeo Kushwaha, Sudhir Raizada
      Several options have been proposed for eradication of germ cells (GCs) in mammals such as treatment with cytotoxic drugs, irradiation, cold ischemia and hyperthermic treatment. Some of these methods have been also tried in fish but conditions for complete sterilization of gonads have not been established. Here, we report the production of sterile adult common carp Cyprinus carpio in 10 weeks by the heat and chemical treatments. The cytotoxic drug busulfan (40mg/kg) was intraperitoneally injected into the animals at 2-week intervals (5 doses in total), and they were maintained in water at 38°C between Weeks 1 and 10. The effectiveness of the treatments was assessed using gonadal index, histology, and vasa gene expression. At the end of Week 10, very severe gonadal degeneration was observed in fish treated with the heat–chemical combination, and 100% of male and female fish were devoid of endogenous GCs. The average levels of vasa transcript were 0.01±0.005 and 0.02±0.016 for males and females, respectively. By contrast, high temperature alone caused minor gonadal degeneration and the gene transcript were 0.59±0.131 for male and 0.62±0.13 for female. In Week 20, after the recovery period of 10 weeks at 25°C, the gonadal germ cell did not recover from the sterile condition in any of the sampled individuals. The change in colouration of gonads was an additional useful index of the degree of gonadal sterility.

      PubDate: 2017-06-07T04:29:24Z
      DOI: 10.1016/j.anireprosci.2017.05.015
  • The expression of progesterone receptor coregulators mRNA and protein in
           corpus luteum and endometrium of cows during the estrous cycle
    • Authors: R Rekawiecki; M.K. Kowalik; J. Kotwica
      Abstract: Publication date: Available online 3 June 2017
      Source:Animal Reproduction Science
      Author(s): R Rekawiecki, M.K. Kowalik, J. Kotwica
      The aim of this study was to examine whether changes in the mRNA and protein expression of the progesterone receptor (PGR) coactivator P300/CBP-associated factor (PCAF) and the corepressor Nuclear Receptor Corepressor 1 (NCOR1) may participate in the regulation of PGR function during the estrous cycle in corpus luteum (CL) and endometrium and thus modulate the effect of progesterone (P4) within the reproductive system. The experimental material included CL and endometrial tissues from cows on days 2-5, 6-10, 11-16, and 17-20 of the estrous cycle. The mRNA expression of PCAF and NCOR1 was determined by means of real-time PCR, and protein levels were determined using western blotting. The highest mRNA and protein expression for PCAF (P < 0.01) and NCOR1 (P < 0.01) was found on days 6-16 in CL, whereas mRNA and protein expression for PCAF in endometrium was the highest on days 1-10 (P < 0.05), but for NCOR1 it was the highest on days 2-5 (P < 0.05) and decreased thereafter. Significant correlations were found between PCAF and NCOR1 mRNA and protein in CL and endometrium, between PCAF mRNA or protein and P4 levels only in CL, and between NCOR1 protein and P4 levels in endometrium only. Correlations between PCAF and NCOR1 mRNA and PCAF and NCOR1 protein were also found. These data suggest that the variable expression of these coregulators in CL and endometrium during the estrous cycle may depend on the influence of P4, and in these tissues both coregulators may compete for binding to the PGR.

      PubDate: 2017-06-07T04:29:24Z
      DOI: 10.1016/j.anireprosci.2017.05.011
  • Reproductive dysfunction in females of endangered golden mahseer (Tor
           putitora) in captivity
    • Authors: M.S. Akhtar; A. Ciji; D. Sarma; M. Rajesh; B.S. Kamalam; P. Sharma; A.K. Singh
      Abstract: Publication date: Available online 18 May 2017
      Source:Animal Reproduction Science
      Author(s): M.S. Akhtar, A. Ciji, D. Sarma, M. Rajesh, B.S. Kamalam, P. Sharma, A.K. Singh
      The present study was undertaken to gain insight on the physiological basis underlying the constraints in attaining maturity of endangered golden mahseer (Tor putitora) in captivity. Selected hormone levels and stress biomarkers were analysed in wild and captive reared brooders to address the above objectives. As compared to their captive counterparts, plasma 17β-estradiol was significantly (p<0.05) higher in wild caught females. A concurrent trend was observed for plasma vitellogenin, aromatase, 17α, 20β-dihydroxy progesterone (17α, 20β DHP), luteinizing hormone (LH) and11-ketotestosterone (11-KT) indicating a weak hormone response in captive females that potentially hindered maturity. To the contrary, the plasma11-KT levels were not significantly different between wild and captive males. Plasma 17α, 20β DHP level was found significantly (p<0.05) higher in wild caught females compared to females reared in captivity. However, both males of wild and captivity registered significantly higher 17α, 20β DHP than captive females. Plasma 11-KT level was significantly higher in males compared to females. However, the captive females had higher level of 11-KT than captive males. Stress biomarkers viz., cortisol, superoxide dismutase (SOD) and glutathione peroxidase (GPx) were also estimated both in wild and captive brooders. There was no significant difference in plasma cortisol levels of wild and captive reared brooders. However, plasma GPx and SOD activity were significantly higher in captive reared T. putitora as compared to wild brooders counterpart manifesting prevailing oxidative stress in captivity. Overall results showed endocrine and stress differences between wild and captive reared brood fishes during early spawning period which highlighted the endocrine failure of female reproductive maturity in captivity.

      PubDate: 2017-05-22T18:46:14Z
      DOI: 10.1016/j.anireprosci.2017.05.004
  • A simple, field-friendly technique for cryopreserving semen from Asian
           elephants (Elephas maximus)
    • Authors: Danielle M. Arnold; Charlie Gray; Terri L. Roth; Sebastian Mitchell; Laura H. Graham
      Abstract: Publication date: Available online 18 May 2017
      Source:Animal Reproduction Science
      Author(s): Danielle M. Arnold, Charlie Gray, Terri L. Roth, Sebastian Mitchell, Laura H. Graham
      The specific objectives of the present study were to investigate the effects of manual seeding, differing freeze and thaw rates as well as storage for 24h at 4°C prior to cryopreservation on post-thaw sperm quality in Asian elephants. Extended semen was cooled in an equitainer to 4°C, frozen in liquid nitrogen vapour at various rates with and without manual seeding or in a dry shipper and thawed at 37, 50 and 75°C. There was a significant effect of freeze rate on post-thaw motility (P<0.0001) and acrosomal integrity (P<0.005). The faster freeze rates in the dry shipper and at 1cm or 2cm above liquid nitrogen consistently provided better cryopreservation than slower freezing rates. Thaw temperature had no effect on post-thaw semen quality but there was an interaction between freeze and thaw rates with higher thaw rates resulting in superior post-thaw semen quality in straws frozen at fast rates. Storage of samples prior to freezing had a detrimental effect on post-thaw semen quality. In summary, our results indicate cooling extended semen in an equitainer and cryopreserving it by placing straws directly in a dry shipper is a simple technique for effectively cryopreserving Asian elephant semen in the field or zoo.

      PubDate: 2017-05-22T18:46:14Z
      DOI: 10.1016/j.anireprosci.2017.05.003
  • Direct effect of curcumin on porcine ovarian cell functions
    • Authors: Attila Kádasi; Nora Maruniaková; Aneta Štochmaľová; Miroslav Bauer; Roland Grossmann; Abdel Halim Harrath; Adriana Kolesárová; Alexander V. Sirotkin
      Abstract: Publication date: Available online 12 May 2017
      Source:Animal Reproduction Science
      Author(s): Attila Kádasi, Nora Maruniaková, Aneta Štochmaľová, Miroslav Bauer, Roland Grossmann, Abdel Halim Harrath, Adriana Kolesárová, Alexander V. Sirotkin
      Curcuma longa Linn (L.) is a plant widely used in cooking (in curry powder a.o.) and in folk medicine, but its action on reproductive processes and its possible mechanisms of action remain to be investigated. The objective of this study was to examine the direct effects of curcumin, the major Curcuma longa L. molecule, on basic ovarian cell functions such as proliferation, apoptosis, viability and steroidogenesis. Porcine ovarian granulosa cells were cultured with and without curcumin (at doses of 0, 1, 10 and 100μg/ml of medium). Markers of proliferation (accumulation of PCNA) and apoptosis (accumulation of bax) were analyzed by immunocytochemistry. The expression of mRNA for PCNA and bax was detected by RT-PCR. Cell viability was detected by trypan blue exclusion test. Release of steroid hormones (progesterone and testosterone) was measured by enzyme immunoassay (EIA). It was observed that addition of curcumin reduced ovarian cell proliferation (expression of both PCNA and its mRNA), promoted apoptosis (accumulation of both bax and its mRNA), reduced cell viability, and stimulated both progesterone and testosterone release. These observations demonstrate the direct suppressive effect of Curcuma longa L./curcumin on female gonads via multiple mechanisms of action − suppression of ovarian cell proliferation and viability, promotion of their apoptosis (at the level of mRNA transcription and subsequent accumulation of promoters of genes regulating these activities) and release of anti-proliferative and pro-apoptotic progesterone and androgen. The potential anti-gonadal action of curcumin should be taken into account by consumers of Curcuma longa L.-containing products.

      PubDate: 2017-05-17T18:33:33Z
      DOI: 10.1016/j.anireprosci.2017.05.001
  • Sperm sexing in Nili-Ravi buffalo through modified swim up: validation
           using SYBR® green real-time PCR
    • Authors: Asma-ul-Husna; Muhammad Amjad Awan; Abid Mehmood; Tasawar Sultana; Qaisar Shahzad; Muhammad Sajjad Ansari; Bushra Allah Rakha; S.M. Saqlan Naqvi; Shamim Akhter
      Abstract: Publication date: Available online 6 May 2017
      Source:Animal Reproduction Science
      Author(s): Asma-ul-Husna, Muhammad Amjad Awan, Abid Mehmood, Tasawar Sultana, Qaisar Shahzad, Muhammad Sajjad Ansari, Bushra Allah Rakha, S.M. Saqlan Naqvi, Shamim Akhter
      Sperm sexing through flow-sorting technology is relatively expensive, requires considerable technical support and is actually not practicable in many developing countries. The aim of this study was to investigate the feasibility of producing enriched pools of X or Y chromosome-bearing sperm by a modified swim-up method. For this purpose semen was collected from five mature Nili-Ravi buffalo bulls for a period of six weeks. The qualifying ejaculates were divided into two aliquots for further processing through modified swim-up or control (untreated). After processing, semen was cryopreserved in tris citric acid extender using standard techniques. Semen quality was assessed at pre dilution, post dilution and post thawing. Validation of technique was done by using SYBR® green real time PCR using two sets of primers, PLP and SRY for X and Y chromosome of buffalo genes, respectively. Sperm recovery rates, pre freeze and post thaw sperm quality were found significantly higher in X chromosome bearing sperm fraction than Y chromosome bearing fraction and control. Mean fold relative expression of X bearing sperm was significantly higher (4-5 fold) in X chromosome bearing fraction of supernatant than Y chromosome bearing fraction (0.06 fold), similarly mean fold relative expression of Y chromosome bearing sperm was significantly higher in Y chromosome bearing fraction (4 fold) of supernatant than X chromosome bearing fraction (0.15 fold) compared to control (1.00). In conclusion, a modified swim up method proved to be an effective method for Nili-Ravi buffalo sperm sexing as validated by real time PCR.

      PubDate: 2017-05-08T18:13:36Z
      DOI: 10.1016/j.anireprosci.2017.04.011
  • Expression of TNF-α system members in bovine ovarian follicles and the
           effects of TNF-α or dexamethasone on preantral follicle survival,
           development and ultrastructure in vitro
    • Authors: A.W.B. Silva; R.P. Ribeiro; V.G. Menezes; R.S. Barberino; J.R.S. Passos; A.M.P. Dau; J.J.N. Costa; L.R.F. Melo; F.T.G. Bezerra; M.A.M. Donato; C.A. Peixoto; M.H.T. Matos; P.B.D Gonçalves; R. van den Hurk; J.R.V. Silva
      Abstract: Publication date: Available online 6 May 2017
      Source:Animal Reproduction Science
      Author(s): A.W.B. Silva, R.P. Ribeiro, V.G. Menezes, R.S. Barberino, J.R.S. Passos, A.M.P. Dau, J.J.N. Costa, L.R.F. Melo, F.T.G. Bezerra, M.A.M. Donato, C.A. Peixoto, M.H.T. Matos, P.B.D Gonçalves, R. van den Hurk, J.R.V. Silva
      This study was conducted to detect the protein expression of TNF-α system members (TNF-α/TNFR1/TNFR2) in bovine ovarian follicles and to evaluate the effects of TNF-α or dexamethasone on the survival and growth of primordial follicles in vitro, as well as on gene expression in cultured ovarian tissue. It was hypothesized that TNF-α induces follicular atresia in ovarian tissues cultured in vitro, and that dexamethasone suppresses the production of endogenous TNF-α, which can improve follicle viability in vitro. Ovarian fragments were cultured for 6days in α-MEM+ supplemented with TNF-α (0, 1, 10, 100 or 200ng/mL) or dexamethasone (0, 1, 10, 100 or 200ng/mL). After culture, the expression of mRNAs for BCL-2, BAX, P53, TNF-α, and CASP3 and CASP6 were evaluated. Immunohistochemical results showed that the TNF-α system members, were detected in bovine preantral and antral follicles. After 6 days, the TNF-α (10ng/ml) treatment reduced the percentage of normal preantral follicles and increased the number of TUNEL-positive cells in cultured tissue. Dexamethasone (10ng/mL) during 6days of culture did maintain the percentage of normal follicles and the ultrastructure of follicles, while the presence of TNF-α or dexamethasone did not influence primordial follicle activation. However, TNF-α or dexamethasone had no effect on the levels of mRNA for P53, BCL-2, BAX and CASP6, in cultured tissues, but the presence of dexamethasone reduced the levels of CASP3 compared to ovarian slices cultured in control medium (α-MEM+). In conclusion, proteins of the TNF-α system are expressed at different bovine follicle stages. The addition of TNF-α in culture reduces follicle survival and increases the number of apoptotic cells in ovarian tissue, while the presence of dexamethasone maintains follicle ultrastructure in cultured tissue.

      PubDate: 2017-05-08T18:13:36Z
      DOI: 10.1016/j.anireprosci.2017.04.010
  • Effects of leptin administration on development, vascularization and
           function of Corpus luteum in alpacas submitted to pre-ovulatory fasting
    • Authors: María Cecilia Norambuena; Francisca Hernández; Jonathan Maureira; Carolina Rubilar; Jorge Alfaro; Gonzalo Silva; Mauricio Silva; César Ulloa-Leal
      Abstract: Publication date: Available online 3 May 2017
      Source:Animal Reproduction Science
      Author(s): María Cecilia Norambuena, Francisca Hernández, Jonathan Maureira, Carolina Rubilar, Jorge Alfaro, Gonzalo Silva, Mauricio Silva, César Ulloa-Leal
      The objective of this study was to determine the effect of leptin administration on the development, vascularization and function of Corpus luteum (CL) in alpacas submitted to pre-ovulatory fasting. Fourteen alpacas were kept in fasting conditions for 72h and received five doses of o-leptin (2μg/kg e.v.; Leptin group) or saline (Control group) every 12h. Ovulation was induced with a GnRH dose (Day 0). The ovaries were examined every other day by trans-rectal ultrasonography (7.5MHz; mode B and power Doppler) from Day 0 to 13 to determine the pre-ovulatory follicle diameter and ovulation, and then to monitor CL diameter and vascularization until the regression phase. Serial blood samples were taken after GnRH treatment to determine plasma LH concentration; and every other day from Days 1 to 13 to determine plasma progesterone and leptin concentrations. The pre-ovulatory follicle and CL diameter, LH, progesterone and leptin plasma concentrations were not affected by treatment (P >0.05). The vascularization area of the CL was, nevertheless, affected by the treatment (P <0.01) with significant differences between groups at Days 3, 7 and 9 (P <0.05). The Leptin group had a larger maximum vascularization area (0.67±0.1 compared with 0.35±0.1cm2; P <0.05). In addition, there was a positive correlation between CL vascularization, CL diameter and plasma progesterone. The exogenous administration of leptin during pre-ovulatory fasting increased the vascularization of the CL in alpacas in vivo.

      PubDate: 2017-05-08T18:13:36Z
      DOI: 10.1016/j.anireprosci.2017.04.006
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