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Publisher: Elsevier   (Total: 3043 journals)

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Showing 1 - 200 of 3043 Journals sorted alphabetically
AASRI Procedia     Open Access   (Followers: 15)
Academic Pediatrics     Hybrid Journal   (Followers: 20, SJR: 1.402, h-index: 51)
Academic Radiology     Hybrid Journal   (Followers: 18, SJR: 1.008, h-index: 75)
Accident Analysis & Prevention     Partially Free   (Followers: 83, SJR: 1.109, h-index: 94)
Accounting Forum     Hybrid Journal   (Followers: 23, SJR: 0.612, h-index: 27)
Accounting, Organizations and Society     Hybrid Journal   (Followers: 27, SJR: 2.515, h-index: 90)
Achievements in the Life Sciences     Open Access   (Followers: 4)
Acta Anaesthesiologica Taiwanica     Open Access   (Followers: 5, SJR: 0.338, h-index: 19)
Acta Astronautica     Hybrid Journal   (Followers: 331, SJR: 0.726, h-index: 43)
Acta Automatica Sinica     Full-text available via subscription   (Followers: 3)
Acta Biomaterialia     Hybrid Journal   (Followers: 25, SJR: 2.02, h-index: 104)
Acta Colombiana de Cuidado Intensivo     Full-text available via subscription   (Followers: 1)
Acta de Investigación Psicológica     Open Access   (Followers: 2)
Acta Ecologica Sinica     Open Access   (Followers: 8, SJR: 0.172, h-index: 29)
Acta Haematologica Polonica     Free   (SJR: 0.123, h-index: 8)
Acta Histochemica     Hybrid Journal   (Followers: 3, SJR: 0.604, h-index: 38)
Acta Materialia     Hybrid Journal   (Followers: 211, SJR: 3.683, h-index: 202)
Acta Mathematica Scientia     Full-text available via subscription   (Followers: 5, SJR: 0.615, h-index: 21)
Acta Mechanica Solida Sinica     Full-text available via subscription   (Followers: 9, SJR: 0.442, h-index: 21)
Acta Oecologica     Hybrid Journal   (Followers: 9, SJR: 0.915, h-index: 53)
Acta Otorrinolaringologica (English Edition)     Full-text available via subscription   (Followers: 1)
Acta Otorrinolaringológica Española     Full-text available via subscription   (Followers: 3, SJR: 0.311, h-index: 16)
Acta Pharmaceutica Sinica B     Open Access   (Followers: 2)
Acta Poética     Open Access   (Followers: 4)
Acta Psychologica     Hybrid Journal   (Followers: 23, SJR: 1.365, h-index: 73)
Acta Sociológica     Open Access  
Acta Tropica     Hybrid Journal   (Followers: 6, SJR: 1.059, h-index: 77)
Acta Urológica Portuguesa     Open Access  
Actas Dermo-Sifiliograficas     Full-text available via subscription   (Followers: 4)
Actas Dermo-Sifiliográficas (English Edition)     Full-text available via subscription   (Followers: 3)
Actas Urológicas Españolas     Full-text available via subscription   (Followers: 4, SJR: 0.383, h-index: 19)
Actas Urológicas Españolas (English Edition)     Full-text available via subscription   (Followers: 2)
Actualites Pharmaceutiques     Full-text available via subscription   (Followers: 5, SJR: 0.141, h-index: 3)
Actualites Pharmaceutiques Hospitalieres     Full-text available via subscription   (Followers: 4, SJR: 0.112, h-index: 2)
Acupuncture and Related Therapies     Hybrid Journal   (Followers: 3)
Ad Hoc Networks     Hybrid Journal   (Followers: 11, SJR: 0.967, h-index: 57)
Addictive Behaviors     Hybrid Journal   (Followers: 15, SJR: 1.514, h-index: 92)
Addictive Behaviors Reports     Open Access   (Followers: 5)
Additive Manufacturing     Hybrid Journal   (Followers: 8, SJR: 1.039, h-index: 5)
Additives for Polymers     Full-text available via subscription   (Followers: 20)
Advanced Drug Delivery Reviews     Hybrid Journal   (Followers: 128, SJR: 5.2, h-index: 222)
Advanced Engineering Informatics     Hybrid Journal   (Followers: 11, SJR: 1.265, h-index: 53)
Advanced Powder Technology     Hybrid Journal   (Followers: 16, SJR: 0.739, h-index: 33)
Advances in Accounting     Hybrid Journal   (Followers: 9, SJR: 0.299, h-index: 15)
Advances in Agronomy     Full-text available via subscription   (Followers: 15, SJR: 2.071, h-index: 82)
Advances in Anesthesia     Full-text available via subscription   (Followers: 25, SJR: 0.169, h-index: 4)
Advances in Antiviral Drug Design     Full-text available via subscription   (Followers: 3)
Advances in Applied Mathematics     Full-text available via subscription   (Followers: 6, SJR: 1.054, h-index: 35)
Advances in Applied Mechanics     Full-text available via subscription   (Followers: 10, SJR: 0.801, h-index: 26)
Advances in Applied Microbiology     Full-text available via subscription   (Followers: 22, SJR: 1.286, h-index: 49)
Advances In Atomic, Molecular, and Optical Physics     Full-text available via subscription   (Followers: 16, SJR: 3.31, h-index: 42)
Advances in Biological Regulation     Hybrid Journal   (Followers: 4, SJR: 2.277, h-index: 43)
Advances in Botanical Research     Full-text available via subscription   (Followers: 3, SJR: 0.619, h-index: 48)
Advances in Cancer Research     Full-text available via subscription   (Followers: 25, SJR: 2.215, h-index: 78)
Advances in Carbohydrate Chemistry and Biochemistry     Full-text available via subscription   (Followers: 9, SJR: 0.9, h-index: 30)
Advances in Catalysis     Full-text available via subscription   (Followers: 5, SJR: 2.139, h-index: 42)
Advances in Cellular and Molecular Biology of Membranes and Organelles     Full-text available via subscription   (Followers: 12)
Advances in Chemical Engineering     Full-text available via subscription   (Followers: 24, SJR: 0.183, h-index: 23)
Advances in Child Development and Behavior     Full-text available via subscription   (Followers: 10, SJR: 0.665, h-index: 29)
Advances in Chronic Kidney Disease     Full-text available via subscription   (Followers: 10, SJR: 1.268, h-index: 45)
Advances in Clinical Chemistry     Full-text available via subscription   (Followers: 28, SJR: 0.938, h-index: 33)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 18, SJR: 2.314, h-index: 130)
Advances in Computers     Full-text available via subscription   (Followers: 16, SJR: 0.223, h-index: 22)
Advances in Developmental Biology     Full-text available via subscription   (Followers: 11)
Advances in Digestive Medicine     Open Access   (Followers: 4)
Advances in DNA Sequence-Specific Agents     Full-text available via subscription   (Followers: 5)
Advances in Drug Research     Full-text available via subscription   (Followers: 22)
Advances in Ecological Research     Full-text available via subscription   (Followers: 41, SJR: 3.25, h-index: 43)
Advances in Engineering Software     Hybrid Journal   (Followers: 25, SJR: 0.486, h-index: 10)
Advances in Experimental Biology     Full-text available via subscription   (Followers: 7)
Advances in Experimental Social Psychology     Full-text available via subscription   (Followers: 40, SJR: 5.465, h-index: 64)
Advances in Exploration Geophysics     Full-text available via subscription   (Followers: 3)
Advances in Fluorine Science     Full-text available via subscription   (Followers: 8)
Advances in Food and Nutrition Research     Full-text available via subscription   (Followers: 47, SJR: 0.674, h-index: 38)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 15)
Advances in Genetics     Full-text available via subscription   (Followers: 15, SJR: 2.558, h-index: 54)
Advances in Genome Biology     Full-text available via subscription   (Followers: 11)
Advances in Geophysics     Full-text available via subscription   (Followers: 6, SJR: 2.325, h-index: 20)
Advances in Heat Transfer     Full-text available via subscription   (Followers: 21, SJR: 0.906, h-index: 24)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 8, SJR: 0.497, h-index: 31)
Advances in Human Factors/Ergonomics     Full-text available via subscription   (Followers: 25)
Advances in Imaging and Electron Physics     Full-text available via subscription   (Followers: 2, SJR: 0.396, h-index: 27)
Advances in Immunology     Full-text available via subscription   (Followers: 35, SJR: 4.152, h-index: 85)
Advances in Inorganic Chemistry     Full-text available via subscription   (Followers: 9, SJR: 1.132, h-index: 42)
Advances in Insect Physiology     Full-text available via subscription   (Followers: 3, SJR: 1.274, h-index: 27)
Advances in Integrative Medicine     Hybrid Journal   (Followers: 5)
Advances in Intl. Accounting     Full-text available via subscription   (Followers: 4)
Advances in Life Course Research     Hybrid Journal   (Followers: 8, SJR: 0.764, h-index: 15)
Advances in Lipobiology     Full-text available via subscription   (Followers: 2)
Advances in Magnetic and Optical Resonance     Full-text available via subscription   (Followers: 9)
Advances in Marine Biology     Full-text available via subscription   (Followers: 16, SJR: 1.645, h-index: 45)
Advances in Mathematics     Full-text available via subscription   (Followers: 10, SJR: 3.261, h-index: 65)
Advances in Medical Sciences     Hybrid Journal   (Followers: 6, SJR: 0.489, h-index: 25)
Advances in Medicinal Chemistry     Full-text available via subscription   (Followers: 5)
Advances in Microbial Physiology     Full-text available via subscription   (Followers: 4, SJR: 1.44, h-index: 51)
Advances in Molecular and Cell Biology     Full-text available via subscription   (Followers: 22)
Advances in Molecular and Cellular Endocrinology     Full-text available via subscription   (Followers: 10)
Advances in Molecular Toxicology     Full-text available via subscription   (Followers: 7, SJR: 0.324, h-index: 8)
Advances in Nanoporous Materials     Full-text available via subscription   (Followers: 4)
Advances in Oncobiology     Full-text available via subscription   (Followers: 3)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 15, SJR: 2.885, h-index: 45)
Advances in Parallel Computing     Full-text available via subscription   (Followers: 7, SJR: 0.148, h-index: 11)
Advances in Parasitology     Full-text available via subscription   (Followers: 7, SJR: 2.37, h-index: 73)
Advances in Pediatrics     Full-text available via subscription   (Followers: 24, SJR: 0.4, h-index: 28)
Advances in Pharmaceutical Sciences     Full-text available via subscription   (Followers: 13)
Advances in Pharmacology     Full-text available via subscription   (Followers: 15, SJR: 1.718, h-index: 58)
Advances in Physical Organic Chemistry     Full-text available via subscription   (Followers: 7, SJR: 0.384, h-index: 26)
Advances in Phytomedicine     Full-text available via subscription  
Advances in Planar Lipid Bilayers and Liposomes     Full-text available via subscription   (Followers: 3, SJR: 0.248, h-index: 11)
Advances in Plant Biochemistry and Molecular Biology     Full-text available via subscription   (Followers: 8)
Advances in Plant Pathology     Full-text available via subscription   (Followers: 5)
Advances in Porous Media     Full-text available via subscription   (Followers: 4)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 18)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 19, SJR: 1.5, h-index: 62)
Advances in Psychology     Full-text available via subscription   (Followers: 60)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 5, SJR: 0.478, h-index: 32)
Advances in Radiation Oncology     Open Access  
Advances in Small Animal Medicine and Surgery     Hybrid Journal   (Followers: 2, SJR: 0.1, h-index: 2)
Advances in Space Research     Full-text available via subscription   (Followers: 343, SJR: 0.606, h-index: 65)
Advances in Structural Biology     Full-text available via subscription   (Followers: 8)
Advances in Surgery     Full-text available via subscription   (Followers: 7, SJR: 0.823, h-index: 27)
Advances in the Study of Behavior     Full-text available via subscription   (Followers: 30, SJR: 1.321, h-index: 56)
Advances in Veterinary Medicine     Full-text available via subscription   (Followers: 15)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 13)
Advances in Virus Research     Full-text available via subscription   (Followers: 5, SJR: 1.878, h-index: 68)
Advances in Water Resources     Hybrid Journal   (Followers: 43, SJR: 2.408, h-index: 94)
Aeolian Research     Hybrid Journal   (Followers: 5, SJR: 0.973, h-index: 22)
Aerospace Science and Technology     Hybrid Journal   (Followers: 307, SJR: 0.816, h-index: 49)
AEU - Intl. J. of Electronics and Communications     Hybrid Journal   (Followers: 8, SJR: 0.318, h-index: 36)
African J. of Emergency Medicine     Open Access   (Followers: 5, SJR: 0.344, h-index: 6)
Ageing Research Reviews     Hybrid Journal   (Followers: 8, SJR: 3.289, h-index: 78)
Aggression and Violent Behavior     Hybrid Journal   (Followers: 405, SJR: 1.385, h-index: 72)
Agri Gene     Hybrid Journal  
Agricultural and Forest Meteorology     Hybrid Journal   (Followers: 15, SJR: 2.18, h-index: 116)
Agricultural Systems     Hybrid Journal   (Followers: 30, SJR: 1.275, h-index: 74)
Agricultural Water Management     Hybrid Journal   (Followers: 38, SJR: 1.546, h-index: 79)
Agriculture and Agricultural Science Procedia     Open Access  
Agriculture and Natural Resources     Open Access   (Followers: 1)
Agriculture, Ecosystems & Environment     Hybrid Journal   (Followers: 53, SJR: 1.879, h-index: 120)
Ain Shams Engineering J.     Open Access   (Followers: 5, SJR: 0.434, h-index: 14)
Air Medical J.     Hybrid Journal   (Followers: 5, SJR: 0.234, h-index: 18)
AKCE Intl. J. of Graphs and Combinatorics     Open Access   (SJR: 0.285, h-index: 3)
Alcohol     Hybrid Journal   (Followers: 9, SJR: 0.922, h-index: 66)
Alcoholism and Drug Addiction     Open Access   (Followers: 6)
Alergologia Polska : Polish J. of Allergology     Full-text available via subscription   (Followers: 1)
Alexandria Engineering J.     Open Access   (Followers: 1, SJR: 0.436, h-index: 12)
Alexandria J. of Medicine     Open Access  
Algal Research     Partially Free   (Followers: 8, SJR: 2.05, h-index: 20)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 3)
Allergologia et Immunopathologia     Full-text available via subscription   (Followers: 1, SJR: 0.46, h-index: 29)
Allergology Intl.     Open Access   (Followers: 4, SJR: 0.776, h-index: 35)
ALTER - European J. of Disability Research / Revue Européenne de Recherche sur le Handicap     Full-text available via subscription   (Followers: 7, SJR: 0.158, h-index: 9)
Alzheimer's & Dementia     Hybrid Journal   (Followers: 48, SJR: 4.289, h-index: 64)
Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring     Open Access   (Followers: 5)
Alzheimer's & Dementia: Translational Research & Clinical Interventions     Open Access   (Followers: 3)
American Heart J.     Hybrid Journal   (Followers: 48, SJR: 3.157, h-index: 153)
American J. of Cardiology     Hybrid Journal   (Followers: 45, SJR: 2.063, h-index: 186)
American J. of Emergency Medicine     Hybrid Journal   (Followers: 38, SJR: 0.574, h-index: 65)
American J. of Geriatric Pharmacotherapy     Full-text available via subscription   (Followers: 6, SJR: 1.091, h-index: 45)
American J. of Geriatric Psychiatry     Hybrid Journal   (Followers: 16, SJR: 1.653, h-index: 93)
American J. of Human Genetics     Hybrid Journal   (Followers: 31, SJR: 8.769, h-index: 256)
American J. of Infection Control     Hybrid Journal   (Followers: 24, SJR: 1.259, h-index: 81)
American J. of Kidney Diseases     Hybrid Journal   (Followers: 33, SJR: 2.313, h-index: 172)
American J. of Medicine     Hybrid Journal   (Followers: 46, SJR: 2.023, h-index: 189)
American J. of Medicine Supplements     Full-text available via subscription   (Followers: 3)
American J. of Obstetrics and Gynecology     Hybrid Journal   (Followers: 191, SJR: 2.255, h-index: 171)
American J. of Ophthalmology     Hybrid Journal   (Followers: 54, SJR: 2.803, h-index: 148)
American J. of Ophthalmology Case Reports     Open Access   (Followers: 3)
American J. of Orthodontics and Dentofacial Orthopedics     Full-text available via subscription   (Followers: 6, SJR: 1.249, h-index: 88)
American J. of Otolaryngology     Hybrid Journal   (Followers: 23, SJR: 0.59, h-index: 45)
American J. of Pathology     Hybrid Journal   (Followers: 26, SJR: 2.653, h-index: 228)
American J. of Preventive Medicine     Hybrid Journal   (Followers: 21, SJR: 2.764, h-index: 154)
American J. of Surgery     Hybrid Journal   (Followers: 34, SJR: 1.286, h-index: 125)
American J. of the Medical Sciences     Hybrid Journal   (Followers: 12, SJR: 0.653, h-index: 70)
Ampersand : An Intl. J. of General and Applied Linguistics     Open Access   (Followers: 5)
Anaerobe     Hybrid Journal   (Followers: 4, SJR: 1.066, h-index: 51)
Anaesthesia & Intensive Care Medicine     Full-text available via subscription   (Followers: 55, SJR: 0.124, h-index: 9)
Anaesthesia Critical Care & Pain Medicine     Full-text available via subscription   (Followers: 10)
Anales de Cirugia Vascular     Full-text available via subscription  
Anales de Pediatría     Full-text available via subscription   (Followers: 2, SJR: 0.209, h-index: 27)
Anales de Pediatría (English Edition)     Full-text available via subscription  
Anales de Pediatría Continuada     Full-text available via subscription   (SJR: 0.104, h-index: 3)
Analytic Methods in Accident Research     Hybrid Journal   (Followers: 2, SJR: 2.577, h-index: 7)
Analytica Chimica Acta     Hybrid Journal   (Followers: 38, SJR: 1.548, h-index: 152)
Analytical Biochemistry     Hybrid Journal   (Followers: 162, SJR: 0.725, h-index: 154)
Analytical Chemistry Research     Open Access   (Followers: 8, SJR: 0.18, h-index: 2)
Analytical Spectroscopy Library     Full-text available via subscription   (Followers: 11)
Anesthésie & Réanimation     Full-text available via subscription   (Followers: 1)
Anesthesiology Clinics     Full-text available via subscription   (Followers: 22, SJR: 0.421, h-index: 40)
Angiología     Full-text available via subscription   (SJR: 0.124, h-index: 9)
Angiologia e Cirurgia Vascular     Open Access  
Animal Behaviour     Hybrid Journal   (Followers: 157, SJR: 1.907, h-index: 126)
Animal Feed Science and Technology     Hybrid Journal   (Followers: 5, SJR: 1.151, h-index: 83)
Animal Reproduction Science     Hybrid Journal   (Followers: 5, SJR: 0.711, h-index: 78)
Annales d'Endocrinologie     Full-text available via subscription   (Followers: 1, SJR: 0.394, h-index: 30)
Annales d'Urologie     Full-text available via subscription  
Annales de Cardiologie et d'Angéiologie     Full-text available via subscription   (SJR: 0.177, h-index: 13)
Annales de Chirurgie de la Main et du Membre Supérieur     Full-text available via subscription  
Annales de Chirurgie Plastique Esthétique     Full-text available via subscription   (Followers: 2, SJR: 0.354, h-index: 22)
Annales de Chirurgie Vasculaire     Full-text available via subscription   (Followers: 1)

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Journal Cover Acta Histochemica
  [SJR: 0.604]   [H-I: 38]   [3 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0065-1281
   Published by Elsevier Homepage  [3043 journals]
  • The effects of 17β-estradiol on blood brain barrier integrity in the
           absence of the estrogen receptor alpha; an in-vitro model
    • Authors: Serap Erdem Kuruca; Sabriye Karadenizli; Kadriye Akgun-Dar; Aysegul Kapucu; Zulal Kaptan; Gulay Uzum
      Abstract: Publication date: Available online 10 August 2017
      Source:Acta Histochemica
      Author(s): Serap Erdem Kuruca, Sabriye Karadenizli, Kadriye Akgun-Dar, Aysegul Kapucu, Zulal Kaptan, Gulay Uzum
      The blood-brain barrier (BBB), which saves the brain from toxic substances, is formed by endothelial cells. It is mainly composed of tight junction (TJ) proteins existing between endothelial cells. Estrogen is an important regulatory hormone of BBB permeability. It protects the BBB before menopause, but may increase BBB permeability with aging. In addition, nitric oxide modulates BBB permeability. Alcohol impairs the integrity of the BBB with oxidants and inflammatory mediators such as iNOS. We investigated the effects of estrogen on BBB integrity in an in vitro BBB model created with ERα-free HUVEC (human umbilical vein endothelial-like cells) to mimics the menopausal period. In vitro BBB model is created with HUVEC/C6 (rat glioma cells) co-culture. The effect of 17β-estradiol on ethanol-induced BBB disruption and change/or increase of iNOS activity, which modulate BBB integrity, were evaluated. Inducibility and functionality of BBB were investigated using transendothelial electrical resistance (TEER) and the expression of proteins TJ proteins (occludin and claudin-1) and iNOS activity by immunostaining. Our results revealed that 17β-estradiol treatment before and after ethanol decrease expression of occludin and claudin-1 and value of TEER which are BBB disrupt indicators. In addition, ethanol and 17β-estradiol separately and pre- and post-ethanol 17β-estradiol treatment increased iNOS expression. Thus our study suggests caution in the use of 17β-estradiol after menopause because 17β-estradiol at this time may both increase the inflammatory process as well as damage the BBB. We think that beneficial effects of 17β-estradiol may be through ERα but it needs further studies.

      PubDate: 2017-08-16T15:18:52Z
      DOI: 10.1016/j.acthis.2017.07.005
  • The impact of aged garlic extract on adriamycin-induced testicular changes
           in adult male Wistar rats
    • Authors: Ashraf Youssef Nasr
      Abstract: Publication date: Available online 5 August 2017
      Source:Acta Histochemica
      Author(s): Ashraf Youssef Nasr
      Adriamycin (ADR), a potent cytotoxic drug, has many adverse effects on different body organs limiting its therapeutic uses. Aged garlic extract (AGE), a garlic preparation, contains many organosulfur compounds with potent antioxidant activity. This investigation was conducted to study the possible protective effects & the underlying the mechanism of AGE on ADR-induced testicular damage. Thirty-six adult male rats were assigned into six groups: a control, AGE-treated (250mg/kg once oral for 14days), ADR-treated (10mg/kg, i.p. once at day 8), AGE (7days before)+ADR (once at day 8), ADR (once on day 8)+AGE (7days after), AGE (14days)+ADR (once at day 8). At day 15; blood samples were collected then the animals were sacrificed and testicular samples were prepared for light and electron microscopic examination. Parenchymal disorganization, cellular degeneration, nuclear apoptosis, mitochondrial degeneration and cytoplasmic vacuolation, decrease count and increase abnormalities of sperms, low testosterone level, high MDA concentration, low GSH level, and decrease GSH-Px, CAT, and SOD activity were recorded in ADR-treated rats. Remarkable histological, biochemical and ultrastructural improvements were observed in the combined AGE plus ADR-treated groups. Thus, AGE can be used as an adjuvant therapy to resume male infertility and weak spermatogenesis induced by cytotoxic drugs or other environmental toxins through its cytoprotective and antioxidant properties.
      Graphical abstract image

      PubDate: 2017-08-06T12:25:11Z
      DOI: 10.1016/j.acthis.2017.07.006
  • Expression of glucose transporter 8 (GLUT8) in spermatogenesis of adult
           boar testes
    • Authors: Huaming Xi; Zhen Zhang; Rui Ji; Hui Shen; Xiaorui Fan; Qinghong Li; Xiyun Bai; Zhixue Cheng; Junping He
      Abstract: Publication date: Available online 4 August 2017
      Source:Acta Histochemica
      Author(s): Huaming Xi, Zhen Zhang, Rui Ji, Hui Shen, Xiaorui Fan, Qinghong Li, Xiyun Bai, Zhixue Cheng, Junping He
      The present study was designed to investigate the glucose transporter 8 (GLUT8) expression and localization in adult boar testis. Localization and expression of GLUT8 were conducted with Western Blotting, immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) methods GLUT8 protein and mRNA were expressed in the boar testes. The results of Western Blotting analysis showed specificity of the antibody for protein of boar testes. The immunohistochemistry results showed that GLUT8 protein mainly localized in spermatocytes, round spermatids and elongated spermatids of the seminiferous tubules in the adult boar testes. And the GLUT8 expression persists during eight stages of boar spermatogenesis. GLUT8 may mainly provide glucose for the later stage of germ cell differentiation in adluminal compartment in adult boar testes. These results suggested that GLUT8 is important for the spermatogenesis in the adult boar testes.

      PubDate: 2017-08-06T12:25:11Z
      DOI: 10.1016/j.acthis.2017.07.004
  • The Feulgen reaction: A brief review and new perspectives
    • Authors: Maria Luiza S. Mello; Benedicto de Campos Vidal
      Abstract: Publication date: Available online 21 July 2017
      Source:Acta Histochemica
      Author(s): Maria Luiza S. Mello, Benedicto de Campos Vidal
      The Feulgen reaction has been proposed by Robert Feulgen and Heinrich Rossenbeck for the identification of DNA nearly a hundred years ago. Since then, many other applications of this cytochemical/topochemical procedure at qualitative and quantitative level have been proposed in relation to DNA and its role in chromatin in human, animal and plant cells. In this article, we briefly review some fundamental aspects of the Feulgen reaction and current applications of such a method in studies of altered chromatin texture, including its association with or preceding changes in transcriptional activities and effect on epigenetic marks. Further perspectives on the use of the Feulgen reaction will depend of the proposal of innovative biological questions in which its reveals appropriate.

      PubDate: 2017-07-28T04:01:54Z
      DOI: 10.1016/j.acthis.2017.07.002
  • Assessment of bone repair in critical-size defect in the calvarium of rats
           after the implantation of tricalcium phosphate beta (β-TCP)
    • Authors: Leonardo de Freitas Silva; Erik Neiva Ribeiro de Carvalho Reis; Tânia Aparecida Barbara; João Paulo Bonardi; Idelmo Rangel Garcia; Paulo Sérgio Perri de Carvalho; Daniela Ponzoni
      Abstract: Publication date: Available online 18 July 2017
      Source:Acta Histochemica
      Author(s): Leonardo de Freitas Silva, Erik Neiva Ribeiro de Carvalho Reis, Tânia Aparecida Barbara, João Paulo Bonardi, Idelmo Rangel Garcia, Paulo Sérgio Perri de Carvalho, Daniela Ponzoni
      Objectives Evaluating the osteoconductive property of tricalcium phosphate beta (β-TCP) in comparison to that of inorganic bovine bone for repair in a critical-size defect in the rat calvarium. Materials and Methods Critical-size defects of 7mm were made with a trephine in the calvaria of 48 Wistar rats. The animals were divided into four groups, and the defects in each group were filled with tricalcium phosphate beta (β-TCP), inorganic bovine bone (Bio-Oss), autogenous bone, or left empty. The animals were euthanized at two different time points (30 and 60days post-operation). All defects were recovered with a absorbable membrane of bovine cortical bone. Histological, histometric, and immunohistochemical (osteocalcin) assessments were carried out at 30 and 60days post-operation. Results At 30days post-operation, all groups showed areas of bone formation, predominantly when autogenous grafts were used. However, there were no statistically significant differences between the treatment groups (p>0.05). After 60days, there were similarities in the bone formation patterns between the β-TCP (26.32±) and Bio-Oss (17.35±) groups (p=0.549). In terms of the immunohistochemical assessment of osteocalcin, the clot group showed light to moderate staining at 30 and 60days. The autogenous group showed moderate staining at 30days and moderate to intense staining after 60days. The Bio-Oss group showed light to moderate staining after 30days and intense staining at 60days. The β-TCP group showed moderate staining at 30 and 60days post-operation. Conclusion β-TCP is a good osteoconductive material with similar effects to those of inorganic bovine bone graft and is suitable for utilization in the repair of bone defects.

      PubDate: 2017-07-20T03:39:45Z
      DOI: 10.1016/j.acthis.2017.07.003
  • FoxP3 and TLR2 in co-expression in oral cancer
    • Authors: H.M. Hussaini; V.P.B. Parachuru; G.J. Seymour; A.M. Rich
      Abstract: Publication date: Available online 17 July 2017
      Source:Acta Histochemica
      Author(s): H.M. Hussaini, V.P.B. Parachuru, G.J. Seymour, A.M. Rich

      PubDate: 2017-07-20T03:39:45Z
      DOI: 10.1016/j.acthis.2017.06.006
  • Is toll-like receptor-2 really inhibiting FoxP3+ TLR2+ cells and enhancing
           the anti-tumour response in oral squamous cell carcinoma'
    • Authors: Xi Zeng; Mao Li; Guang-dong Liao; Ming-Rong Xi
      Abstract: Publication date: Available online 14 July 2017
      Source:Acta Histochemica
      Author(s): Xi Zeng, Mao Li, Guang-dong Liao, Ming-Rong Xi

      PubDate: 2017-07-20T03:39:45Z
      DOI: 10.1016/j.acthis.2017.06.005
  • The effects of estradiol valerate and remifemin on liver lipid metabolism
    • Authors: Biao Jin; Wenjuan Wang; Wenpei Bai; Jing Zhang; Ke Wang; Lihua Qin
      Abstract: Publication date: Available online 11 July 2017
      Source:Acta Histochemica
      Author(s): Biao Jin, Wenjuan Wang, Wenpei Bai, Jing Zhang, Ke Wang, Lihua Qin
      To investigate the lipid metabolism dysregulation in the liver of ovariectomized (OVX) rats and effects of estradiol valerate (E) and remifemin (ICR) thereon, forty female Sprague-Dawley rats were randomly divided into sham-operated (SHAM), OVX, OVX+E, and OVX+ICR group. After 4 weeks’ E or ICR treatment, serum estrogen, cholesterol, and triglyceride levels; lipid droplets in hepatocytes; hepatocyte morphology; and the expression of estrogen receptor α (ERα), liver X receptor (LXR), and sterol regulatory element binding proteins (SREBPs) in the liver of the rats were assessed. OVX rats had significantly decreased serum estrogen levels, which significantly increased after treatment with E but not with ICR. Serum triglyceride levels and the amount of lipid droplets in hepatocytes increased after ovariectomy, and significantly decreased after E treatment. In addition, ICR treatment markedly increased serum triglyceride levels and lipid droplet size. No significant differences in the serum cholesterol levels were observed among the four groups. After ovariectomy, hepatocyte mitochondria became hypertrophic and misformed, which were reversed with E or ICR treatment. ICR-treated rats also showed endoplasmic reticulum disorganization. After ovariectomy, ERα and LXR levels significantly decreased while SREBP expression increased. E treatment increased ERα and LXR levels while ICR treatment only increased LXR expression. E treatment decreased SREBP-1c levels, whereas SREBP-1c levels increased with ICR treatment. Treatment with E significantly reversed the ovariectomy-induced dysregulation of hepatocyte lipid metabolism, which was, however, exacerbated with ICR treatment. The effects of E and ICR on hepatocyte lipid metabolism may involve the regulation of LXR and SREBP-1c.

      PubDate: 2017-07-20T03:39:45Z
      DOI: 10.1016/j.acthis.2017.06.004
  • Cells in 3D-reconstitutued eccrine sweat gland cell spheroids
           differentiate into gross cystic disease fluid protein 15-expressing dark
           secretory cells and carbonic anhydrase II-expressing clear secretory cells
    • Authors: Haihong Li; Liyun Chen; Mingjun Zhang; Bingna Zhang
      Abstract: Publication date: Available online 6 July 2017
      Source:Acta Histochemica
      Author(s): Haihong Li, Liyun Chen, Mingjun Zhang, Bingna Zhang
      Secretory coils of eccrine sweat glands are composed of myoepithelial cells, dark secretory cells and clear secretory cells. The two types of cells play important roles in sweat secretion. In our previous study, we demonstrated that the 3D-reconstituted eccrine sweat gland cell spheroids differentiate into secretory coil-like structures. However, whether the secretory coil-like structures further differentiate into dark secretory cells and clear secretory cells were is still unknown. In this study, we detected the differentiation of clear and dark secretory cells in the 3D-reconstituted eccrine sweat gland cell spheroids using the dark secretory cell-specific marker, GCDFP-15, and clear secretory cell-specific marker, CAII by immunofluorescence staining. Results showed that there were both GCDFP-15- and CAII-expressing cells in 12-week-old 3D spheroids, similar to native eccrine sweat glands, indicating that the spheroids possess a cellular structure capable of sweat secretion. We conclude that the 12-week 3D spheroids may have secretory capability.

      PubDate: 2017-07-09T16:55:03Z
      DOI: 10.1016/j.acthis.2017.07.001
  • Editorial Board ((ofc))
    • Abstract: Publication date: June 2017
      Source:Acta Histochemica, Volume 119, Issue 5

      PubDate: 2017-07-09T16:55:03Z
  • Leptin-like immunoreactivity in the central nervous system, digestive
           organs, and gonads of the giant freshwater prawn, Macrobrachium
    • Authors: Jaruwan Poljaroen; Yotsawan Tinikul; Ruchanok Tinikul; Panat Anurucpreeda; Prasert Sobhon
      Abstract: Publication date: Available online 16 June 2017
      Source:Acta Histochemica
      Author(s): Jaruwan Poljaroen, Yotsawan Tinikul, Ruchanok Tinikul, Panat Anurucpreeda, Prasert Sobhon
      Leptin, a highly conserved adipocyte-derived hormone, plays important roles in a variety of physiological processes, including the control of fat storage and metabolic status which are linked to food intake, energy homeostasis, and reproduction in all vertebrates. In the present study, we hypothesize that leptin is also present in various organs of the fresh water prawns, Macrobrachium rosenbergii. The existence and distribution of a leptin-like peptide in prawn tissues were verified by using Western blotting (WB) and immunohistochemical detection (ID) using primary antibody against human leptin. With WB, a leptin-like peptide, having a molecular weight of 15kDa, was detected in the brain, thoracic ganglia, abdominal ganglia, parts of the gastro-intestinal tract, hepatopancreas, adipocytes and gonads. By ID, leptin immunoreactivity (leptin-ir) was detected in the brain, thoracic ganglia and intersegmental commissural nerve fibers of abdominal ganglia. In the gastrointestinal tract, there was intense leptin-ir in the apical part of the epithelial cells of the cardiac and pyloric parts of the stomach. In the midgut and hindgut, the leptin-ir was detected in epithelial cells and basal cells located near the basal lamina of the epithelium. In addition, there was leptin-ir in the Restzellen cells in the hepatopancreas which produce digestive enzymes. In the ovary, the strong intensity of a leptin-ir was detected in the cytoplasm of middle to late stage oocytes, whereas no positive staining was detected in follicular cells. An intense leptin-ir was detected in spermatocytes and sustentacular cells in the seminiferous tubules in the testes of small and orange claw males. Taken together, the detection of the leptin-ir in several organs implicates the existence of a leptin-like peptide in various organs of the freshwater prawn; and like in vertebrates this peptide may be an important hormonal factor in controlling feeding and reproductive process.

      PubDate: 2017-06-21T06:40:03Z
      DOI: 10.1016/j.acthis.2017.06.001
  • The effect of caloric restriction on the forelimb skeletal muscle fibers
           of the hypertrophic myostatin null mice
    • Authors: Mohamed I. Elashry; Antonios Matsakas; Sabine Wenisch; Stefan Arnhold; Ketan Patel
      Abstract: Publication date: Available online 15 June 2017
      Source:Acta Histochemica
      Author(s): Mohamed I. Elashry, Antonios Matsakas, Sabine Wenisch, Stefan Arnhold, Ketan Patel
      Skeletal muscle mass loss has a broad impact on body performance and physical activity. Muscle wasting occurs due to genetic mutation as in muscular dystrophy, age-related muscle loss (sarcopenia) as well as in chronic wasting disorders as in cancer cachexia. Food restriction reduces muscle mass underpinned by increased muscle protein break down. However the influence of dietary restriction on the morphometry and phenotype of forelimb muscles in a genetically modified myostatin null mice are not fully characterized. The effect of a five week dietary limitation on five anatomically and structurally different forelimb muscles was examined. C57/BL6 wild type (Mstn+/+ ) and myostatin null (Mstn −/−) mice were either given a standard rodent normal daily diet ad libitum (ND) or 60% food restriction (FR) for a 5 week period. M. triceps brachii Caput laterale (T.lateral), M. triceps brachii Caput longum (T.long), M. triceps brachii Caput mediale (T.medial), M. extensor carpi ulnaris (ECU) and M. flexor carpi ulnaris (FCU) were dissected, weighted and processed for immunohistochemistry. Muscle mass, fibers cross sectional areas (CSA) and myosin heavy chain types IIB, IIX, IIA and type I were analyzed. We provide evidence that caloric restriction results in muscle specific weight reduction with the fast myofibers being more prone to atrophy. We show that slow fibers are less liable to dietary restriction induced muscle atrophy. The effect of dietary restriction was more pronounced in Mstn −/− muscles to implicate the oxidative fibers compared to Mstn+/+ . Furthermore, peripherally located myofibers are more susceptible to dietary induced reduction compared to deep fibers. We additionally report that dietary restriction alters the glycolytic phenotype of the Mstn −/− into the oxidative form in a muscle dependent manner. In summary our study shows that calorie restriction alters muscle fiber profile of forelimb muscles of Myostatin null mice.

      PubDate: 2017-06-21T06:40:03Z
      DOI: 10.1016/j.acthis.2017.06.002
  • The impact of black seed oil on tramadol-induced hepatotoxicity:
           Immunohistochemical and ultrastructural study
    • Authors: Nesreen Moustafa Omar; Mohammed Amin Mohammed
      Abstract: Publication date: Available online 12 June 2017
      Source:Acta Histochemica
      Author(s): Nesreen Moustafa Omar, Mohammed Amin Mohammed
      The natural herb, black seed (Nigella Sativa; NS) is one of the most important elements of folk medicine. The aim was to evaluate the impact of Nigella Sativa Oil (NSO) on the changes induced by tramadol in rat liver. Twenty four albino rats were used. Control group: given intraperitoneal and oral saline for 30days. TR-group: given intraperitoneal tramadol (20, 40, 80mg/kg/day) in the first, middle and last 10days of the experiment, respectively. TR + NS group: administered intraperitoneal tramadol in similar doses to TR-group plus oral NSO (4ml/kg/day) for 30days. Immunohistochemical, electron microscopic, biochemical and statistical studies were performed. TR-group displayed disarranged hepatic architecture, hepatic congestion, hemorrhage and necrosis. Apoptotic hepatocytes, mononuclear cellular infiltration and a significant increase in the number of anti-CD68 positive cells were observed. Ultrastructurally, hepatocytes showed shrunken nuclei, swollen mitochondria, many lysosomes and autophagic vacuoles. Activated Ito and Von Kupffer cells were also demonstrated. Elevated serum levels of AST, ALT, ALP and bilirubin were noticed. NSO administration resulted in preservation of hepatic histoarchitecture and ultrastructure and significant reductions in the number of anti-CD68 positive cells and serum levels of liver seromarkers. In conclusion, NSO administration could mitigate the alterations induced by tramadol in rat liver.

      PubDate: 2017-06-15T06:36:19Z
      DOI: 10.1016/j.acthis.2017.05.008
  • Neuroprotective potential of Aloe arborescens against copper induced
           neurobehavioral features of Parkinson’s disease in rat
    • Authors: Abdellatif Abbaoui; Omar El Hiba; Halima Gamrani
      Abstract: Publication date: Available online 12 June 2017
      Source:Acta Histochemica
      Author(s): Abdellatif Abbaoui, Omar El Hiba, Halima Gamrani
      Copper (Cu) is an important trace element for the organism survival, which ensures the normal functioning of different biosystems. However, excessive levels of this heavy metal are responsible for profound physiological alterations including the central nervous system. Numerous findings sustain the involvement of heavy metals, as an environmental risk factor such as copper (Cu), in the neuropathology of Parkinson’s disease (PD) which is a chronic neurodegenerative disorder that principally affects the motor system. The classic and evident symptoms of PD namely rigidity, tardiness of movement, and difficulty with walking, result from progressive dopaminergic neurons death within substantia nigra. Whereas, few pharmacological trials have shown a beneficial role against Cu neurotoxicity, Aloe arborescens is one of the powerful medicinal plants with an array of therapeutic effects. Thus, we aimed through the present study, to evaluate the impact of acute Cu intoxication (10μg/g B.W. i.p) for 3days on the dopaminergic system and locomotor performance, together with the possible restorative effect of oral administration of aqueous extract of Aloe arborescens gel (AEAAG) (200mg/kg B.W.). By means of immunohistochemistry, we noted, in the Cu intoxicated rats, a significant loss of TH (tyrosine hydroxylase) expression within substantia nigra compacta (SNc), ventral tegmental area (VTA) and the subsequent striatal outputs, those alterations were correlated to behavioral abnormalities such as a severe drop of locomotor performance. While AEAAG administration to Cu intoxicated rats showed a noticeable beneficial effect; this potential was featured by a complete recovery of the TH expression and locomotor behavior deficiencies in the intoxicated rats. The present investigation have brought, on the one hand, an experimental evidence of an altered dopaminergic innervations following Cu intoxication and on the other hand, a new pharmacological property of Aloe arborescens that may be used as a neuroprotective plant for neurodegenerative disorders, such as PD, touching the dopaminergic system trigged by heavy metals.

      PubDate: 2017-06-15T06:36:19Z
      DOI: 10.1016/j.acthis.2017.06.003
  • Expression patterns of p38αMAPK during follicular development in the
           ovaries of neonatal rats
    • Authors: Shifu Hu; Meng Rao; Hui Lei; Yanqing Wu; Yingying Wang; Dandan Ke; Wei Xia; Changhong Zhu
      Abstract: Publication date: Available online 10 June 2017
      Source:Acta Histochemica
      Author(s): Shifu Hu, Meng Rao, Hui Lei, Yanqing Wu, Yingying Wang, Dandan Ke, Wei Xia, Changhong Zhu
      The p38αMAPK signaling pathway plays a critical role in female reproduction, but an understanding of its expression in rats remains elusive. This study was carried out to investigate the temporal and spatial expression of p38αMAPK and p-p38αMAPK. Ovarian tissue samples were collected from 2-, 4-, 8-, 12-, 16-, 20- and 30-day-old female rats. Western blotting was used to examine the relative expression of p38αMAPK and p-p38αMAPK in ovarian tissue, and subcellular localization was examined using immunohistochemistry of the rat ovaries at different ages of postpartum. The immunohistochemical results showed that p38αMAPK and p-p38αMAPK were widely expressed in the rat ovaries, mainly localized in the follicle cells and granulosa cells. The expression of p38αMAPK was relatively stable for the different stages of oocytes, whereas the expression of p-p38αMAPK gradually increased. At different stages of granulosa cells, the expression of p38αMAPK was also relatively stable, and the p-p38αMAPK expression showed an upward trend during follicular development. Western blotting revealed that the expression of p38αMAPK in the ovaries was relatively stable, where as p-p38αMAPK expression initially exhibited an increasing trend and subsequently decreased, with a maximum at day 20. The expression patterns of p38αMAPK and p-p38αMAPK in the rat ovaries indicate their possible involvement in folliculogenesis. Taken together, the stage- and cell-specific expression of p-p38αMAPK in rat ovaries indicated that p-p38αMAPK might play a vital role during rat follicular development.

      PubDate: 2017-06-11T06:33:26Z
      DOI: 10.1016/j.acthis.2017.05.007
  • A histomorphometric study on the effects of antiretroviral therapy (ART)
           combined with a high-calorie diet (HCD) on aortic perivascular adipose
           tissue (PVAT)
    • Authors: S. Nel; H. Strijdom; A. Genis; F. Everson; R. Van Wijk; S.H. Kotzé
      Abstract: Publication date: Available online 9 June 2017
      Source:Acta Histochemica
      Author(s): S. Nel, H. Strijdom, A. Genis, F. Everson, R. Van Wijk, S.H. Kotzé
      Perivascular adipose tissue (PVAT), surrounding arteries is metabolically active. Obesity and antiretroviral therapy (ART) may cause pathophysiological conditions in the aortic wall and surrounding PVAT. The aim of the study was to determine the histological effects on the aortic wall, aortic PVAT adipocyte morphology and leptin staining intensity in obese rats treated with ART. Wistar rats (N=36) were divided into four groups; a lean control (C/ART-), ART control (C/ART+), high-calorie diet (HCD) untreated (HCD/ART-) and HCD and ART experimental (HCD/ART+). The aorta and surrounding PVAT were stained with haematoxylin and eosin (H&E) and anti-leptin antibodies for immunohistochemistry (IHC). The C/ART+ group had a thinner tunica media compared to the HCD/ART- group. The tunica adventitia was thicker in the ART groups (C/ART+ and HCD/ART+) compared to the lean control group. White adipocytes in the HCD/ART- group was larger in size compared to the other three groups. The high-calorie diet groups (HCD/ART- and HCD/ART+) had increased adipocyte sizes, for both brown and differentiating adipocytes, compared to the control groups (C/ART- and C/ART+). The unilocular and differentiating adipocytes in the C/ART+ group showed intense leptin staining. Unilocular and differentiating adipocytes in the HCD/ART- and HCD/ART+ groups showed weak to no leptin staining intensity. The present study indicated that ART and a HCD, separately and combined, altered both the tunica media and adventitia of the aortic wall, whereas the HCD alone caused adipocytes to increase in size. The leptin staining intensity suggested that ART alone may lead to increased leptin expression, whereas ART combined with a HCD may cause leptin deficiency. Changes seen with ART in a rat model suggest that aortic wall thickness and PVAT adipocyte morphology alterations should be considered by clinicians in obese individuals receiving ART.

      PubDate: 2017-06-11T06:33:26Z
      DOI: 10.1016/j.acthis.2017.05.009
  • Squamous epithelium formation in the respiratory intestine of the bronze
           Corydoras Corydoras aeneus (Callichthyidae Teleostei)
    • Authors: Leszek Satora; Katarzyna Kozioł; Jacek Zebrowski
      Abstract: Publication date: Available online 8 June 2017
      Source:Acta Histochemica
      Author(s): Leszek Satora, Katarzyna Kozioł, Jacek Zebrowski
      Accessory respiratory organs in fish exhibit great diversity but share the presence of numerous capillaries covered by a simple squamous epithelium. The adoption of the intestine for respiratory function needs certain special modifications. In this study, we explored immunohistochemical and metabolic fingerprint features that could underlay this adaptation in bronze corydoras Corydoras aeneus. Immunohistochemical localization of the cytoplasmic domain of epidermal growth factor receptor (EGFR) in the respiratory part of intestine demonstrated a strong positive immunoreaction in epithelial cells and connective tissue. Fourier Transfer Infrared (FTIR) spectroscopy coupled with chemometrics discriminated between anterior and posterior region of intestine in terms of secondary structure of proteins and the abundance of p-cresol and other phenolics. The latter were reduced in the posterior part of intestine, indicating the cessation of digestive function in this region. It has been suggested that aquatic hypoxia via endocrine cells (hypoxia-sensitive) activate EGFR, which induce proliferation of squamous epithelial cells, thereby enabling gas diffusion in the posterior part of intestine. It seems that hypoxia and normoxia are opposed conditions adjusting the production of squamous epithelial cells in this intestine. The physiological role of EGFR in the respiratory intestine of bronze corydoras is of interest not only from an evolutionary aspect but also in terms of a potential model for observations process proliferation squamous epithelial cells. Future investigations on the molecular responses to different water oxygen levels in air-breathing bronze corydoras fish are required to clarify the mechanism responsible for squamous cell proliferation.

      PubDate: 2017-06-11T06:33:26Z
      DOI: 10.1016/j.acthis.2017.05.010
  • Movat Pentachrom stain reveals unexpected high osteogenesis rate in aortic
    • Authors: Claudia Dittfeld; Michael Haase; Maria Feilmeier; Anett Jannasch; Petra Büttner; Katrin Plötze; Thomas Waldow; Sems-Malte Tugtekin
      Abstract: Publication date: Available online 2 June 2017
      Source:Acta Histochemica
      Author(s): Claudia Dittfeld, Michael Haase, Maria Feilmeier, Anett Jannasch, Petra Büttner, Katrin Plötze, Thomas Waldow, Sems-Malte Tugtekin
      Background and aim of the study Aortic valve (AV) stenosis is the most common valvular heart disease with an incidence of 3% for people ≥ 65years in the industrialized world with indication for a surgical or transcatheter valve replacement. Researchers suppose osteogenic processes as key mechanisms in calcific aortic valve stenosis. Recently, Torre et al. published impressive histological analyses and detected osseous and/or chondromatous metaplasia in 15.6% of 6685 native calcified aortic valves. Therefore one HE section per valve originated from the area with the greatest extent of calcification was analyzed. Aim of our experimental setup was to identify regions of neo-osteogenesis and to determine the rate of specimens with active mineralization in human aortic valve tissue by Movat Pentachrom staining of sections of lager tissue segments. Methods Operational replaced aortic valves of 35 patients, 15 female and 20 male with an average age of 66.2 years were formalin fixed and decalcified using Osteosoft®-solution. Tissue samples were cut and 2μm specimens were stained with Movat Pentachrom to visualize osteogenic regions. Instead of screening a large number of sections, tissue samples were cut up to five times with at least 100μm space each if no region of osseous and/or chondromatous metaplasia was visible. Results/Conclusions Using this setup, a region of osseous metaplasia was detected in 25 (71.4%) of 35 samples analyzed. In some cases, these regions were small sized and only visible due to the bright color of Movat Pentachrom stain. This leads to the suggestion that a higher rate of calcified aortic valve samples would be classified as cusps with areas of neo-osteogenesis after staining with Movat Pentachrom stain and by the systematic analysis of larger parts of the tissue blocks.

      PubDate: 2017-06-06T06:29:26Z
      DOI: 10.1016/j.acthis.2017.05.006
  • Endocannabinoids modulate apoptosis in endometriosis and adenomyosis
    • Authors: Elif Bilgic; Elif Guzel Meydanli; Sevil Kose; Makbule Cisel Aydin; Eda Karaismailoglu; Irem Akar; Alp Usubutun; Petek Korkusuz
      Abstract: Publication date: Available online 23 May 2017
      Source:Acta Histochemica
      Author(s): Elif Bilgic, Elif Guzel Meydanli, Sevil Kose, Makbule Cisel Aydin, Eda Karaismailoglu, Irem Akar, Alp Usubutun, Petek Korkusuz
      Adenomyosis that is a form of endometriosis is the growth of ectopic endometrial tissue within the muscular wall of the uterus (myometrium), which may cause dysmenorrhea and infertility. Endocannabinoid mediated apoptotic mechanisms of endometriosis and adenomyosis are not known. We hypothesized that the down regulation of endocannabinoid receptors and/or alteration in their regulatory enzymes may have a direct role in the pathogenesis of endometriosis and adenomyosis through apoptosis. Endocannabinoid receptors CB1 and CB2, their synthesizing and catabolizing enzymes (FAAH, NAPE-PLD, DAGL, MAGL) and the apoptotic indexes were immunohistochemically assessed in endometriotic and adenomyotic tissues. Findings were compared to normal endometrium and myometrium. Endometrial adenocarcinoma (Ishikawa) and ovarian endometriosis cyst wall stromal (CRL-7566) cell lines were furthermore cultured with or without cannabinoid receptor agonists. The IC50 value for CB1 and CB2 receptor agonists was quantified. Cannabinoid agonists on cell death were investigated by Annexin-V/Propidium iodide labeling with flow cytometry. CB1 and CB2 receptor levels decreased in endometriotic and adenomyotic tissues compared to the control group (p=0,001 and p=0,001). FAAH, NAPE-PLD, MAGL and DAGL enzyme levels decreased in endometriotic and adenomyotic tissues compared to control (p=0,001, p=0,001, p=0,001 and p=0,002 respectively). Apoptotic cell indexes both in endometriotic and adenomyotic tissues also decreased significantly, compared to the control group (p=0,001 and p=0,001). CB1 and CB2 receptor agonist mediated dose dependent fast anti-proliferative and pro-apoptotic effects were detected in Ishikawa and ovarian endometriosis cyst wall stromal cell lines (CRL-7566). Endocannabinoids are suggested to increase apoptosis mechanisms in endometriosis and adenomyosis. CB1 and CB2 antagonists can be considered as potential medical therapeutic agents for endometriosis and adenomyosis.

      PubDate: 2017-05-27T06:21:39Z
      DOI: 10.1016/j.acthis.2017.05.005
  • Ultrastructural analysis of human leukemia U-937 cells after apoptosis
           induction: Localization of proteasomes and perichromatin fibers
    • Authors: Ekaterina S. Snigirevskaya; Yan Yu. Komissarchik
      Abstract: Publication date: Available online 22 May 2017
      Source:Acta Histochemica
      Author(s): Ekaterina S. Snigirevskaya, Yan Yu. Komissarchik
      We studied the ultrastructure of human histiocytic lymphoma U-937cells after apoptosis induction with two external agents, hypertonic shock and etoposide. Appearance of aggregates of particles of nuclear origin within the nuclei and cytoplasm of the induced cells was the first and the most prominent morphological sign of apoptosis. These aggregates were not coated by a membrane, had variable shape, density and size. Two types of particles dominated in the aggregates: perichromatin fibers (PFs) and proteasomes (PRs). PFs represent a morphological expression of transcriptional and co-transcriptional processing of pre-mRNA (Biggiogera et al., 2008), PRs are involved in hydrolysis of proteins and nucleoproteins, and participate in regulation of apoptosis (Ciechanover, 1998; Liu et al., 2007). We examined the ultrastructure and localization of PFs and PRs, and confirmed the proteasome nature of the latter by immunoelectron microscopy. We traced the formation and migration of the aggregates along the nucleus and their exit into the cytoplasm across the nuclear pores. Finally, we demonstrated degradation of the aggregates and relocating their content into exosomes at the terminal stages of apoptosis with aid of exosomes. We suggest that proteasomes function as morphologically definite and independent intracellular organelles. Alongside with proteasomes, autophagic vacuoles were revealed in apoptotic cells. Occurrence of autophagic vacuoles in apoptotic cells may suggest that both proteolytic pathways, autophagy and proteasome degradation, are coordinated with each other along the programmed cell death pathway.

      PubDate: 2017-05-27T06:21:39Z
      DOI: 10.1016/j.acthis.2017.04.011
  • Identification of synaptic pattern of NMDA receptor subunits upon
           direction-selective retinal ganglion cells in developing and adult mouse
    • Authors: Jun-Seok Lee; Hang-Gu Kim; Chang-Jin Jeon
      Abstract: Publication date: Available online 22 May 2017
      Source:Acta Histochemica
      Author(s): Jun-Seok Lee, Hang-Gu Kim, Chang-Jin Jeon
      Direction selectivity of the retina is a unique mechanism and critical function of eyes for surviving. Direction-selective retinal ganglion cells (DS RGCs) strongly respond to preferred directional stimuli, but rarely respond to the opposite or null directional stimuli. These DS RGCs are sensitive to glutamate, which is secreted from bipolar cells. Using immunocytochemistry, we studied with the distributions of N-methyl-d-aspartate (NMDA) receptor subunits on the dendrites of DS RGCs in the developing and adult mouse retina. DS RGCs were injected with Lucifer yellow for identification of dendritic morphology. The triple-labeled images of dendrites, kinesin II, and NMDA receptor subunits were visualized using confocal microscopy and were reconstructed from high-resolution confocal images. Although our results revealed that the synaptic pattern of NMDA receptor subunits on dendrites of DS RGCs was not asymmetric in developing and adult mouse retina, they showed the anatomical connectivity of NMDA glutamatergic synapses onto DS RGCs and the developmental formation of the direction selectivity in the mouse retina. Through the comprehensive interpretation of the direction-selective neural circuit, this study, therefore, implies that the direction selectivity may be generated by the asymmetry of the excitatory glutamatergic inputs and the inhibitory inputs onto DS RGCs.

      PubDate: 2017-05-27T06:21:39Z
      DOI: 10.1016/j.acthis.2017.05.002
  • Localization and distribution of gonadal proteins in the oviparous lizard
           Sceloporus aeneus (Squamata: Phrynosomatidae)
    • Authors: Antonio-Rubio Nivia Rocio; Villagrán-SantaCruz Maricela; Moreno-Mendoza Norma
      Abstract: Publication date: Available online 14 May 2017
      Source:Acta Histochemica
      Author(s): Antonio-Rubio Nivia Rocio, Villagrán-SantaCruz Maricela, Moreno-Mendoza Norma
      Among vertebrates, several specific proteins are involved in the function and development of gonads. Several genes such as SOX9, FOXL2, DDX4, IFITM3, and DPPA3, are active during embryonic differentiation and maintain their expression in adult tissues, playing important roles in the function and development of the line cell, where these are produced. Among reptiles, molecular mechanisms for sex differentiation have been analyzed in turtles, crocodiles, and some lizards, while in adult stages such studies are scarce. The aim of this study was to locate and analyze the distribution of important gonadal proteins in adult and embryonic ovaries and testes of the oviparous lizard Sceloporus aeneus (Squamata: Phrynosomatidae). Adult specimens and embryos of the lizard S. aeneus were collected in Milpa Alta, a suburb located Southwest of Mexico City. Expression of gonadal proteins was analyzed using immunofluorescent staining and confocal microscopy. Our results showed that SOX9 is located in Sertoli cells of embryonic and adult testes. FOXL2 is expressed in follicular cells of adult ovaries. DDX4 and IFITM3 are located in germ line cells as well as in follicular cells of adult ovaries. DPPA3 was observed in somatic and germ line cells of adult and embryonic gonads. Our observations show that important molecules of vertebrate ovaries and testes are conserved in S. aeneus and it is suggested that these may have a similar role during gonadal development and function.

      PubDate: 2017-05-17T06:14:47Z
      DOI: 10.1016/j.acthis.2017.05.004
  • Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob
    • Authors: Fabiane Ferreira Martins; Marcia Barbosa Aguila; Carlos Alberto Mandarim-de-Lacerda
      Abstract: Publication date: Available online 13 May 2017
      Source:Acta Histochemica
      Author(s): Fabiane Ferreira Martins, Marcia Barbosa Aguila, Carlos Alberto Mandarim-de-Lacerda
      The obesity and its comorbidities, including resistance to leptin, impacts the reproductive function. Testes express leptin receptors in the germ cells and Leydig cells. Then, leptin-deficient animals are obese and infertile. We aimed to evaluate the structure and steroidogenic pathway of the testis of deficient leptin mice. Three months old male C57BL/6 mice (wild-type, WT) and deficient leptin (ob/ob) mice had their testes dissected and prepared for analyses. Compared to the WT group, the ob/ob group showed a greater body mass with smaller testes, and alterations in the germinative epithelium: fewer spermatogonia, spermatocytes, and spermatids. The Sertoli cells and the germ cells showed condensed nuclei and nuclear fragmentation indicating cell death, in agreement with a low expression of the proliferating cell nuclear antigen and a high expression of Caspase3. In the ob/ob group, the sperm was absent in the seminiferous tubules, and the steroidogenic pathway was compromised (low 3Beta hydroxysteroid dehydrogenase and steroidogenic acute regulatory protein). Further, all hormone receptors involved in the testicular function were down expressed (androgen, estrogen, follicle-stimulating, luteinizing, aromatase, and nicotinamide adenine dinucleotide phosphate). In conclusion, the findings indicate significant morphological, hormonal and enzymatic changes in the testis of the ob/ob mice. The shifts in the enzymatic steroidogenic pathway and the enzymes related to spermatic activity support the insights about the failures in the fertility of these animals. The study provides new evidence and contributes to the understanding of how the lack of leptin and obesity might negatively modulate the testicular function leading to infertility.

      PubDate: 2017-05-17T06:14:47Z
      DOI: 10.1016/j.acthis.2017.05.003
  • Apoptosis of bovine granulosa cells: Intracellular pathways and
    • Authors: M.C. Carou; P.R. Cruzans; A. Maruri; M.G. Farina; C.D. Fiorito; G. Olea; D.M. Lombardo
      Abstract: Publication date: Available online 12 May 2017
      Source:Acta Histochemica
      Author(s): M.C. Carou, P.R. Cruzans, A. Maruri, M.G. Farina, C.D. Fiorito, G. Olea, D.M. Lombardo
      Follicular atresia in granulosa and theca cells occurs by apoptosis through weak hormonal stimulation. We have previously proposed an in vitro model to study this process by inducing apoptosis in BGC-1, a bovine granulosa cell line, and in primary cultures from ovaries with or without corpus luteum (CPGB+ and CPGB−, respectively), with different doses of gonadotropin releasing hormone (GnRH) analogs (leuprolide acetate (LA) as agonist and antide as antagonist). BGC-1 represent immature granulosa cells, whereas CPGB represent different degrees of luteinization. Our aim was to evaluate the intracellular pathways involved in the GnRH regulation of apoptosis in BGC-1. Treatment with LA 100nM but not with antide led to an increase in BAX over BCL-2 expression, showing antagonism of antide. All treatments inhibited phospholipase-D (PLD) activity compared to control, implying agonist behavior of antide. Progesterone in vitro production and 3β-hydroxysteroid dehydrogenase (3β-HSD) expression revealed different degrees of luteinization: BGC-1 were immature, whereas CPGB+ were less differentiated than CPGB−. We concluded that LA-induced apoptosis in BGC-1 occurs by activation of the mitochondrial pathway and by inhibition of PLD activity and that antide might work both as an antagonist of the intrinsic pathway and as an agonist of the extrinsic protection pathway by inhibiting PLD activity.

      PubDate: 2017-05-17T06:14:47Z
      DOI: 10.1016/j.acthis.2017.04.010
  • Editorial Board ((ofc))
    • Abstract: Publication date: May 2017
      Source:Acta Histochemica, Volume 119, Issue 4

      PubDate: 2017-05-17T06:14:47Z
  • Submucosal neurons and enteric glial cells expressing the P2X7 receptor in
           rat experimental colitis
    • Authors: Marcos Vinícius da Silva; Aline Rosa Marosti; Cristina Eusébio Mendes; Kelly Palombit; Patricia Castelucci
      Abstract: Publication date: Available online 11 May 2017
      Source:Acta Histochemica
      Author(s): Marcos Vinícius da Silva, Aline Rosa Marosti, Cristina Eusébio Mendes, Kelly Palombit, Patricia Castelucci
      The aim of this study was to evaluate the effect of ulcerative colitis on the submucosal neurons and glial cells of the submucosal ganglia of rats. 2,4,6-Trinitrobenzene sulfonic acid (TNBS; colitis group) was administered in the colon to induce ulcerative colitis, and distal colons were collected after 24h. The colitis rats were compared with those in the sham and control groups. Double labelling of the P2X7 receptor with calbindin (marker for intrinsic primary afferent neurons, IPANs, submucosal plexus), calretinin (marker for secretory and vasodilator neurons of the submucosal plexus), HuC/D and S100β was performed in the submucosal plexus. The density (neurons per area) of submucosal neurons positive for the P2X7 receptor, calbindin, calretinin and HuC/D decreased by 21%, 34%, 8.2% and 28%, respectively, in the treated group. In addition, the density of enteric glial cells in the submucosal plexus decreased by 33%. The profile areas of calbindin-immunoreactive neurons decreased by 25%. Histological analysis revealed increased lamina propria and decreased collagen in the colitis group. This study demonstrated that ulcerative colitis affected secretory and vasodilatory neurons, IPANs and enteric glia of the submucosal plexus expressing the P2X7 receptor.

      PubDate: 2017-05-12T06:10:39Z
      DOI: 10.1016/j.acthis.2017.05.001
  • Sesamin encouraging effects on chondrogenic differentiation of human
           amniotic fluid-derived mesenchymal stem cells
    • Authors: Suteera Narakornsak; Sirinda Aungsuchawan; Peeraphan Pothacharoen; Runchana Markmee; Waleephan Tancharoen; Tanongsak Laowanitwattana; Chawapon Thaojamnong; Lamaiporn Peerapapong; Nonglak Boonma; Witoon Tasuya; Junjira Keawdee; Naree Poovachiranon
      Abstract: Publication date: Available online 9 May 2017
      Source:Acta Histochemica
      Author(s): Suteera Narakornsak, Sirinda Aungsuchawan, Peeraphan Pothacharoen, Runchana Markmee, Waleephan Tancharoen, Tanongsak Laowanitwattana, Chawapon Thaojamnong, Lamaiporn Peerapapong, Nonglak Boonma, Witoon Tasuya, Junjira Keawdee, Naree Poovachiranon
      Worldwide, the most recognized musculoskeletal degenerative disease is osteoarthritis (OA). Sesamin, a major abundant lignan compound present in Sesamun Indicum Linn, has been described for its various pharmacological effects and health benefits. However, the promoting effects of sesamin on chondrogenic differentiation have not yet been observed. Herein, the aim of this study was to investigate the effects of sesamin on cell cytotoxicity and the potent supporting effects on chondrogenic differentiation of human amniotic fluid-derived mesenchymal stem cells (hAF-MSCs). The results indicated that sesamin was not toxic to hAF-MSCs after sesamin treatment. When treating the cells with a combination of sesamin and inducing factors, sesamin was able to up-regulate the expression level of specific genes which play an essential role during the cartilage development process, including SOX9, AGC, COL2A1, COL11A1, and COMP and also simultaneously promote the cartilage extracellular protein synthesis, aggrecan and type II collagen. Additionally, histological analysis revealed a high amount of accumulated sGAG staining inside the porous scaffold in the sesamin co-treating group. In conclusion, the results of this study have indicated that sesamin can be considered a chondrogenic inducing factor and a beneficial dietary supplement for cartilage repair.

      PubDate: 2017-05-12T06:10:39Z
      DOI: 10.1016/j.acthis.2017.04.006
  • Potential involvement of chondroitin sulfate A in the pathogenesis of
    • Authors: Xiangjun Li; Hiroshi Kurita; Tiepeng Xiao; Kyou Iijima; Kenji Kurashina; Jun Nakayama
      Abstract: Publication date: Available online 9 May 2017
      Source:Acta Histochemica
      Author(s): Xiangjun Li, Hiroshi Kurita, Tiepeng Xiao, Kyou Iijima, Kenji Kurashina, Jun Nakayama
      Ameloblastoma is classified as a benign odontogenic tumor characterized by locally invasive behavior and high risk of recurrence. Here, we evaluate a potential role for glycosaminoglycan, a structural component of cell membranes and extracellular matrix, in ameloblstoma pathogenesis. We subjected formalin-fixed, paraffin-embedded tissue sections of 34 cases of ameloblastoma, 10 of odontogenic keratocyst, and 17 of dentigerous cyst to immunohistochemistry using monoclonal antibodies recognizing chondroitin sulfate A (CS-A), heparan sulfate (HS), and keratan sulfate (KS). Expression levels of CS-A in epithelial component and stroma of ameloblastoma were significantly higher than those in odontogenic keratocyst and dentigerous cyst. Moreover, CS-A in ameloblastoma was more strongly expressed in stellate reticulum-like cells than in amelobast-like cells with statistical significance. On the other hand, expression levels of HS and KS in epithelial component and stroma of ameloblastoma were lower compared with CS-A. These results overall reveal that among these odontogenic lesions, CS-A is preferentially expessed in ameloblastoma, suggesting potential pathogenetic role probably in cytodifferention of tumor cells to stellate reticulum-like cells.

      PubDate: 2017-05-12T06:10:39Z
      DOI: 10.1016/j.acthis.2017.04.001
  • In vivo effects of Allium cepa L. on the selected gut microflora and
           intestinal histomorphology in broiler
    • Authors: Sajid ur Rahman; Sarzamin Khan; Naila Chand; Umar Sadique; Rifat Ullah Khan
      Abstract: Publication date: Available online 9 May 2017
      Source:Acta Histochemica
      Author(s): Sajid ur Rahman, Sarzamin Khan, Naila Chand, Umar Sadique, Rifat Ullah Khan
      The experiment was conducted to evaluate the effect of onion (Allium cepa L.,) on the performance, gut microflora and intestinal histomorphology of broiler chicks. Three hundred and twenty day old broiler chicks (Ross) were randomly divided into four groups as A, B, C and D. Group A served as control without any supplementation, group B, C and D was supplemented with onion powder at the rate of 1.5, 2 and 2.5g/kg of feed respectively. Supplementation of onion significantly (P<0.05) increased weight gain and feed consumption. Supplementation of onion showed a significant (P<0.05) reduction in the population of E. coli and increased significantly Lactobacillus and Streptococcus species. Onion supplementation significantly (P<0.05) increased the morphometry of length, width, crypt depth and surface area of the villus in duodenum, jejunum and ileum of the small intestine. In conclusion, onion supplementation in broiler produced a positive effect on performance, gut microflora and intestinal histomorphology.

      PubDate: 2017-05-12T06:10:39Z
      DOI: 10.1016/j.acthis.2017.04.004
  • Immunocytochemical localization of cholinergic amacrine cells in the bat
    • Authors: Eun-Bee Park; Ya-Nan Gu; Chang-Jin Jeon
      Abstract: Publication date: Available online 5 May 2017
      Source:Acta Histochemica
      Author(s): Eun-Bee Park, Ya-Nan Gu, Chang-Jin Jeon
      The purpose of this study was to localize the cholinergic amacrine cells, one of the key elements of a functional retina, in the retina of a microbat, Rhinolophus ferrumequinum. The presence and localization of choline acetyltransferase-immunoreactive (ChAT-IR) cells in the microbat retina were investigated using immunocytochemistry, confocal microscopy, and quantitative analysis. These ChAT-IR cells were present in the ganglion cell layer (GCL) and inner part of the inner nuclear layer (INL), as previously reported in various animals. However, the bat retina also contained some ChAT-IR cells in the outer part of the INL. The dendrites of these cells extended into the outer plexiform layer, and those of the cells in the inner INL extended within the outer part of the inner plexiform layer (IPL). The dendrites of the ChAT-IR cells in the GCL extended into the middle of the IPL and some fibers ramified up to the outer IPL. The average densities of ChAT-IR cells in the GCL, inner INL, and outer INL were 259±31cells/mm2, 469±48cells/mm2, and 59±8cells/mm2, respectively. The average total density of the ChAT-IR cells was 788±58cells/mm2 (mean±S.D.; n=3; 2799±182 cells/retina). We also found that the cholinergic amacrine cells in the bat retina contained calbindin, one of the calcium-binding proteins, but not calretinin or parvalbumin. As the cholinergic amacrine cells play key roles in the direction selectivity and optokinetic eye reflex in the other mammalian retinas, the present study might provide better information of the cytoarchitecture of bat retina and the basic sources for further physiological studies.

      PubDate: 2017-05-07T06:07:46Z
      DOI: 10.1016/j.acthis.2017.04.009
  • Spatial distribution of cannabinoid receptor 1 and fatty acid amide
           hydrolase in the cat ovary and oviduct
    • Authors: Andrea Pirone; Carla Lenzi; Angela Briganti; Francesco Abbate; Maria Levanti; Francesca Abramo; Vincenzo Miragliotta
      Abstract: Publication date: Available online 4 May 2017
      Source:Acta Histochemica
      Author(s): Andrea Pirone, Carla Lenzi, Angela Briganti, Francesco Abbate, Maria Levanti, Francesca Abramo, Vincenzo Miragliotta
      Involvement of the endocannabinoid system in female reproduction has been extensively described in humans with the cognate receptors and ligands being found in the ovaries and genital tract. In human, an imbalance of the endocannabinoid system is linked with both ectopic pregnancy and infertility. In bovine species anandamide levels regulate aspects of sperm-oviduct interaction. Here we report the immunohistochemical distribution of cannabinoid receptor 1 (CB1R) and fatty acid amide hydrolase (FAAH) in cat ovary and oviduct, using paraffin-embedded tissue samples and commercially available antibodies. We found a differential expression of both CB1R and FAAH during different stages of ovarian function and in the oviduct. CB1R was detected only in tertiary follicle granulosa cells while more immature follicles were negative. FAAH was instead found in ovarian pre-antral follicles, the oocyte cytoplasm, and in granulosa cells of primary, secondary and tertiary follicles. Secondary and tertiary follicles were also FAAH immunoreactive. Luteal cells were immunopositive for both CB1R and FAAH. Because CBR1 in oviduct was found only in ciliated cells, it might represent a specific marker at least in cats. In contrast, FAAH immunoreactivity was observed in both ciliated and non-ciliated cells. The present study may thus serve as the starting point for further investigations on the role of the endocannabinoid system in cat reproduction. Additional work will be needed to assess whether the morphological distribution of CB1R and FAAH changes in different conditions such as pre-pubertal age, follicular phase of the sexual cycle and pregnancy.

      PubDate: 2017-05-07T06:07:46Z
      DOI: 10.1016/j.acthis.2017.04.007
  • Acute infection with an avirulent strain of Toxoplasma gondii causes
           decreasing and atrophy of nitrergic myenteric neurons of rats
    • Authors: Débora de Mello Gonçales Sant’Ana; Marcelo Biondaro Gois; Catchia Hermes-Uliana; Letícia Sarturi Pereira-Severi; Emily Martins Baptista; Luana Colognese Mantovani; Aristeu Vieira da Silva; Eduardo José de Almeida Araújo
      Abstract: Publication date: Available online 4 May 2017
      Source:Acta Histochemica
      Author(s): Débora de Mello Gonçales Sant’Ana, Marcelo Biondaro Gois, Catchia Hermes-Uliana, Letícia Sarturi Pereira-Severi, Emily Martins Baptista, Luana Colognese Mantovani, Aristeu Vieira da Silva, Eduardo José de Almeida Araújo
      In the enteric nervous system (ENS), nitrergic neurons produce and use nitric oxide (NO) as an inhibitory motor neurotransmitter in response to parasitic infections, including those caused by Toxoplasma gondii. However, damage to the host caused by NO has been reported by various authors, and the role of NO in protection or cytotoxicity continues to be extensively studied. In this study, nitrergic neurons were investigated in the myenteric plexus of the jejunum and the distal colon of rats infected with 500 oocysts of the M7741 strain of T. gondii. Ten rats were randomly assigned into a control group (CG) and infected group (IG; received 500 sporulated oocysts of T. gondii orally). After 24h, the rats were euthanized, and samples of the jejunum and distal colon were obtained and processed for NADPH-diaphorase histochemical analysis. Quantitative and morphometric analysis of the nitrergic neurons in whole mounts containing the myenteric plexus was performed. There was a numeric reduction of nitrergic neurons per mm2 in both jejunum and distal colon. The remaining nitrergic neurons suffered atrophy in the areas of the cell body and nucleus, which resulted in a decrease in cytoplasm. Thus, we conclude that an avirulent strain of T. gondii in a short time causes neuroplastic changes in the small and large intestine of rats.
      Graphical abstract image

      PubDate: 2017-05-07T06:07:46Z
      DOI: 10.1016/j.acthis.2017.04.008
  • Polar and apolar extra virgin olive oil and leaf extracts as a promising
           anti-inflammatory natural treatment for osteoarthritis
    • Authors: Houda Nsir; Marta Anna Szychlinska; Venera Cardile; Adriana Carol Eleonora Graziano; Rosanna Avola; Hanen Esafi; Alessandra Bendini; Mokhtar Zarouk; Carla Loreto; Venerando Rapisarda; Paola Castrogiovanni; Giuseppe Musumeci
      Abstract: Publication date: Available online 29 April 2017
      Source:Acta Histochemica
      Author(s): Houda Nsir, Marta Anna Szychlinska, Venera Cardile, Adriana Carol Eleonora Graziano, Rosanna Avola, Hanen Esafi, Alessandra Bendini, Mokhtar Zarouk, Carla Loreto, Venerando Rapisarda, Paola Castrogiovanni, Giuseppe Musumeci
      Osteoarthritis (OA) is a common joint disease with important socio-economic impact. Looking for a novel natural treatment represents an important challenge and a public health objective. A wide range of previous studies highlighted the antioxidant and anti-inflammatory properties especially of phenolic compounds present in olive oil. Therefore, the aim of this study was to investigate the anti-inflammatory activity of Tunisian Extra Virgin Olive Oil (EVOO) extracts in an in vitro OA model. To this aim we evaluated the polar and apolar olive oil fractions and olive leaf extract. The antioxidant capacity of these extracts was determined by using DPPH and FRAP assays. For the in vitro study, the human OA chondrocytes were exposed to lipopolysaccharide (LPS) to induce inflammatory response. The inducible isoform of nitric oxide syntheses (iNOS) and collagen type II expressions were evaluated by immunocytochemistry and western blot analysis. Principal outcome showed that the extracts had important anti-inflammatory potential by decreasing iNOS synthesis in OA chondrocytes. The anti-inflammatory effect was dependent on the extract composition and the used dose. An insignificant effect was seen on collagen type II expression. These results show that nutritional components contained in Tunisian EVOO influence the cellular metabolism, suggesting their potential therapeutic use in patients with inflammatory joint diseases.
      Graphical abstract image

      PubDate: 2017-05-01T06:03:25Z
      DOI: 10.1016/j.acthis.2017.04.005
  • ILK regulates MSCs survival and angiogenesis partially through AKT and
           mTOR signaling pathways
    • Authors: Bin Zeng; Lei Liu; Shaofeng Wang; Zhiguo Dai
      Abstract: Publication date: Available online 28 April 2017
      Source:Acta Histochemica
      Author(s): Bin Zeng, Lei Liu, Shaofeng Wang, Zhiguo Dai
      Mesenchymal stem cells (MSCs) exert therapeutic effects on treating acute myocardial infarction (AMI). Angiogenesis in ischemic heart can promote the supply of oxygen and nutrients to both ischemic myocardium and transplanted stem cells. Focus is then given to the evolving strategies amied at angiogenesis. ILK has been reported to be an important factor regulating apoptosis and angiogenesis. This study examined the role and mechanism of ILK in MSCs survival and angiogenesis. In hypoxic condition, upregulation of ILK expression increased the phosphorylation of Akt and mTOR, resulting in markedly enchanced MSCs survival and VEGF expression; while significantly inhibited MSCs survival and VEGF expression was detected in MSCs with ILK kinase inactivation, which was associated with a reduction of phosphorylation of Akt and mTOR. In addition, it also caused an inhibitory effects of ILK on MSCs survival and VEGF expression, which was abolished by Akt or mTOR inhibitor. Furthermore, it was observed that ILK-overexpressed MSCs increased MSCs survival at 4days and angiogenesis at 3 weeks after transplantation into infracted myocardium as compared with GFP-MSCs group and ILK-SiRNA-MSCs group. This enhanced response was associated with attenuated left ventricular (LV) chamber dilation, reduced LV fibrosis, decreased infarct size and improved LV function. These findings reveal ILK play a pivotal role in regulating MSCs survival and VEGF expression partially through Akt and mTOR signaling pathway. In addition, transplantation of ILK-overexpressed MSCs into infracted myocardium resulted in reduced fibrosis, improved cardiac function and remodeling, which mainly medicated through increased MSCs survival and angiogenesis.

      PubDate: 2017-05-01T06:03:25Z
      DOI: 10.1016/j.acthis.2017.04.003
  • Nanomicelle curcumin-induced DNA fragmentation in testicular tissue;
           Correlation between mitochondria dependent apoptosis and failed
           PCNA-related hemostasis
    • Authors: Sana Moshari; Vahid Nejati; Gholamreza Najafi; Mazdak razi
      Abstract: Publication date: Available online 4 April 2017
      Source:Acta Histochemica
      Author(s): Sana Moshari, Vahid Nejati, Gholamreza Najafi, Mazdak razi
      Current study was done to assess possible anti-proliferative effect of nanomicelle curcumin (NMCM) against germ cells in testicular tissue. For this purpose, 24 mature male Wistar rats were divided into control and test groups. The animals in test groups received 7.5mg/kg, 15mg/kg and 30mg/kg of NMC (NO=6 rats in each group). Following 48days, the expression of Bcl-2, Bax, caspase-3, P53 and proliferating cell nuclear antigen (PCNA) were evaluated by using reverse transcription-PCR and immunohistochemistry. Histological changes, tubular differentiation index (TDI), tissue cellularity and serum level of testosterone were analyzed. Finally, the DNA laddering test was used to assess the DNA fragmentation as hallmark for apoptosis. The NMCM significantly (P<0.05) diminished the Bcl-2, p53 and PCNA and enhanced the Bax and caspase-3 mRNA levels. The NMCM significantly (P<0.05) elevated the percentage of Bax and caspase-3-positive tubules and remarkably reduced the percentage of tubules with positive reaction for Bcl-2, p53 and PCNA. The NCMN-received animals exhibited remarkable (P<0.05) reduction in cell population, TDI ratio and serum level of testosterone. Severe DNA fragmentation was observed in 30mg/kg NMCM-received group. In conclusion, the NMCM by reducing the testicular endocrine status, down-regulating Bcl-2 expression and by enhancing the Bax and caspase-3 expression initiates the intrinsic apoptosis pathway. On the other hand, inhibited expression of p53 and PCNA (at dose level of 30mg/kg) suppresses the p53 and PCNA-related hemostasis/preservative reactions. All these alterations adversely affect the spermatogenesis.
      Graphical abstract image

      PubDate: 2017-05-01T06:03:25Z
      DOI: 10.1016/j.acthis.2017.03.007
  • Resveratrol improves mitochondrial function in the remnant kidney from 5/6
           nephrectomized rats
    • Authors: Yan Hui; Miaomiao Lu; Yarong Han; Hongli Zhou; Wei Liu; Lijing Li; Ruixia Jin
      Abstract: Publication date: Available online 20 April 2017
      Source:Acta Histochemica
      Author(s): Yan Hui, Miaomiao Lu, Yarong Han, Hongli Zhou, Wei Liu, Lijing Li, Ruixia Jin
      Mitochondrial dysfunction is involved in the pathogenesis of chronic kidney disease (CKD). Resveratrol has been demonstrated to be beneficial for the recovery of kidney diseases. In this study, the 5/6 nephrectomized rat was used as a CKD model and the TGF-β1-exposed mouse mesangial cells were used as an in vitro model. Pathological examination showed that resveratrol treatment attenuated glomerular injury in the remnant kidney of 5/6 nephrectomized rat. Additionally, resveratrol improved mitochondrial function in vivo and in vitro, as evidenced by increasing mitochondrial membrane potential, increasing ATP, decreasing reactive oxygen species production and enhancing activities of complex I and III. Furthermore, the dysregulated expressions of electron transport chain proteins and fission/fusion proteins in the kidney of 5/6 nephrectomize rats and TGF-β1-exposed mesangial cells were restored by resveratrol. Finally, upregulated sirt1 and PGC-1α deacetylation were found after treatment with resveratrol in vivo and in vitro, which may contribute to the mitochondrial protective effects of resveratrol. The results demonstrate that resveratrol protects the mitochondria of kidney in 5/6 nephrectomized rats and TGF-β1 induced mesangial cells. The study provides new insights into the renoprotective mechanisms of resveratrol.

      PubDate: 2017-04-24T05:58:07Z
      DOI: 10.1016/j.acthis.2017.04.002
  • Seasonal variations of aromatase and estrogen receptors expression in the
           testis of free-ranging sand rats
    • Authors: Rafik Menad; Souaâd Smaï; Xavier Bonnet; Thérèse Gernigon-Spychalowicz; Elara Moudilou; Farida Khammar; Jean-Marie Exbrayat
      Abstract: Publication date: Available online 17 April 2017
      Source:Acta Histochemica
      Author(s): Rafik Menad, Souaâd Smaï, Xavier Bonnet, Thérèse Gernigon-Spychalowicz, Elara Moudilou, Farida Khammar, Jean-Marie Exbrayat
      An increasing number of studies revealed the importance of estrogen in male reproduction. However, most research was conducted in laboratory rodents subjected to standardized environmental conditions. Therefore, seasonal regulations of estrogen pathways remain poorly understood under natural conditions. Using immunohistochemistry, the expression of several molecules involved in the functioning of testis (i.e. 17-β estradiol [E2], P450 aromatase, estrogen receptors ESR1, ESR2, and GPER1 [also known as GPR30]) were investigated in free-ranging fat sand rats, Psammomys obesus, during the breeding and resting seasons. Leydig cells showed a strong immunoreactivity for aromatase in the testis sampled during the breeding season only; however, E2, ESR1, ESR2 and GPER1 were present during both seasons. Sertoli cells showed a positive signal for E2 and ESR2 during the breeding season; though, all molecules, except GPER1, were present during the resting season. Spermatogonia were reactive for E2, ESR2 and GPER1 during the breeding season and for ESR1 and GPER1 during the resting season. During both seasons, spermatocytes-I presented a moderate reactivity for E2, ESR1, ESR2 and a strong reactivity for GPER1; aromatase was detected during the resting season only. Spermatids and spermatozoa were present exclusively during breeding season and were reactive for all molecules; except round spermatids that were negative for aromatase. The functioning of the testis depends on finely tuned stimulation and inhibition systems. Our results suggest that differential expression of aromatase, ESR1, ESR2, and GPER1 across cells types is involved in the seasonal activation/inactivation cycle of spermatogenesis in a free-ranging species.

      PubDate: 2017-04-24T05:58:07Z
      DOI: 10.1016/j.acthis.2017.03.008
  • Editorial Board ((ofc))
    • Abstract: Publication date: April 2017
      Source:Acta Histochemica, Volume 119, Issue 3

      PubDate: 2017-04-17T04:45:13Z
  • Seeds in the liver
    • Authors: Hongjie Ji; Yanrong Lu; Yujun Shi
      Abstract: Publication date: Available online 4 April 2017
      Source:Acta Histochemica
      Author(s): Hongjie Ji, Yanrong Lu, Yujun Shi
      The liver is a crucial organ for homeostasis and has a tremendous self-renewal and regenerative capacity. It has long been believed that the self-renewal and repair of the liver within a given physiological condition or its repopulation in chronic liver diseases, when hepatocyte proliferation is impaired, will primarily be conducted by the proliferating duct cells, termed “oval cells” or hepatic progenitor cells (HPCs). In addition, numerous studies have revealed that HPCs are the initial tumor cells of liver cancer under certain micro-environments. However, benefit from the extensive application of lineage tracing strategies using the Cre/LoxP system, researchers have redefined the fate of these bipotential cells, raising obvious controversies regarding the capacity of liver cells to control their own biology and differentiation. Here, we review the relevant articles, focusing on cell-lineage tracing to better understanding seed cells and their distinct fate in the liver.

      PubDate: 2017-04-10T04:38:05Z
      DOI: 10.1016/j.acthis.2017.03.006
  • Differential effect of chronic undernutrition on the fiber type
           composition of fascicles in the extensor digitorum longus muscles of the
    • Authors: Enrique Vázquez-Mendoza; Erika Elizabeth Rodríguez-Torres; Kenia López-García; Cindy Xilonen Hinojosa-Rodríguez; Ismael Jiménez-Estrada
      Abstract: Publication date: Available online 2 April 2017
      Source:Acta Histochemica
      Author(s): Enrique Vázquez-Mendoza, Erika Elizabeth Rodríguez-Torres, Kenia López-García, Cindy Xilonen Hinojosa-Rodríguez, Ismael Jiménez-Estrada
      Several studies have shown that chronic low food consumption alters the composition and metabolism of the extensor digitorum longus muscle (EDLm) fiber types. EDLm is constituted by four independent fascicles (F2–F5) of different sizes; their constitution and metabolism, however, as well as how chronic undernourishment affects these is virtually unknown. Thus, the aim of this study is to evaluate the relative fiber type composition and metabolism of each independent fascicle in the EDLm, using control and chronically undernourished young male rats by using the alkaline ATPase and NADH-TR histochemical techniques. Our results indicate that all control fascicles showed a higher percentage of intermediate fibers (P<0.001), except for F5, which had a higher percentage of fast fibers (P<0.001). After chronic undernutrition, the proportion of intermediate fibers decreased in F4 (P<0.05) and increased in F5 (P<0.001), whereas fast fibers decreased in F3 (P<0.05) and F5 (P<0.001). When we investigated fiber metabolism we found that F3 and F4 had a similar composition (mainly glycolytic), whereas F2 and F5 predominantly contained oxidative fibers. All fascicles of chronic undernourished rats showed a general decrease in oxidative fibers (P>0.05), except for F3, in which oxidative fibers increased (P<0.05). After determining the possible predominant metabolism expressed in intermediate fibers, we propose that chronic undernutrition induces the transformation of fast-glycolytic to intermediate-oxidative/glycolytic fibers, mainly in F3 and F5. Our observations confirm that chronic undernourishment differentially affects the fiber types of each fascicle in the EDLm, which could alter their individual physiological contractile properties.

      PubDate: 2017-04-03T04:33:07Z
      DOI: 10.1016/j.acthis.2017.03.005
  • Pulmonary apoptotic and oxidative damaging effects of Triclosan alone or
           in combination with Fluoride in Sprague Dawley rats
    • Authors: Amany Tharwat Mohammed; Amany Abdel-Rahman Mohamed; Haytham Ali
      Abstract: Publication date: Available online 2 April 2017
      Source:Acta Histochemica
      Author(s): Amany Tharwat Mohammed, Amany Abdel-Rahman Mohamed, Haytham Ali
      This study aimed to assess the potential apoptotic and oxidative damaging impacts of Triclosan (TCS) and Sodium Fluoride (NaF) administered separately or in combination, in rats for thirty days. For this purpose, forty immature female Sprague-Dawley rats were equally allocated into five groups. TCS group administered 185mg TCS/kg Bw dissolved in distilled water (DW), while its control group received only DW. NaF group was given 50mg/kg NaF in corn oil BW and its respective control had corn oil alone. The co-treated group was administered TCS and NaF. The oxidative stress biomarkers were evaluated in lung tissue homogenate and apoptotic proteins (BcL-2 and Caspase-3) expression were quantified in lung tissues. The results of TCS or NaF treated groups revealed a prominent depletion of super oxide dismutase (SOD), Catalase (CAT) and Glutathione (GSH) in lung tissue homogenate. On contrary, a marked increase in the tissue levels of Malondialdehyde (MDA) and lactate dehydrogenase (LDH) enzymatic assay levels. The co-exposed group evoked less severity in the oxidative stress biomarkers concentration than individually exposed groups. The apoptotic genes protein expression was significantly higher in TCS or NaF treated rats when compared to the control with intense to moderate immunolabeling of the bronchiolar lining epithelium and surrounding mononuclear inflammatory cells. On the contrary, no significant differences were detected in the expression of the investigated apoptotic biomarkers between the control and the combined exposed group. We concluded that the exposure to either TCS or NaF resulted in significant perturbations in lung tissue after short term oral administration at variable instances but the co-exposure resulted in less severe toxicological consequences.

      PubDate: 2017-04-03T04:33:07Z
      DOI: 10.1016/j.acthis.2017.03.004
  • Germ cell apoptosis and expression of Bcl-2 and Bax in porcine testis
           under normal and heat stress conditions
    • Authors: Xiaorui Fan; Huaming Xi; Zhen Zhang; Yajun Liang; Qinghong Li; Junping He
      Abstract: Publication date: Available online 6 March 2017
      Source:Acta Histochemica
      Author(s): Xiaorui Fan, Huaming Xi, Zhen Zhang, Yajun Liang, Qinghong Li, Junping He
      The aim of this study was to examine whether an elevated ambient temperature (37–40°C) had an effect on the apoptosis of germ cells and the expression of Bcl-2 and Bax in porcine testis. Six boars were used. Three boars were subjected to an elevated ambient temperature (37–40°C, 7days, 3h per day) as a heat stress (HS) group. The other 3 boars were kept in a room temperature house (20–27°C) as a control group. All boars were castrated and the testes were harvested. TUNEL assay was used for the detection of apoptotic cells. Immunohistochemistry, Western blotting and quantitative real-time PCR were used to analyze protein and mRNA levels of Bcl-2 and Bax in response to heat treatment. The results showed that apoptotic signals increased under heat stress conditions compared with the control (P<0.01), and the cell types most affected by heat treatment were spermatocytes and spermatids. In both the control and experimental groups, Bcl-2 was expressed in the cytoplasm and nucleus of spermatogonia, spermatocytes and differentiating spermatids and Bcl-2 preferentially localized close to the seminiferous tubule's luminal surface in late spermatocytes and spermatids. Compared with the control group, the expression levels of Bcl-2 protein and mRNA significantly increased in heat treatment group, while the expression levels of Bax protein and mRNA did not show significant changes between the control and experimental group. Low to moderate Bax immunoreactivity staining was observed in all kinds of germ cells in the control group. Strong staining was observed in spermatogonia, and low to moderate Bax staining was observed in spermatocytes and spermatids. A redistribution of Bax from a cytoplasmic to perinuclear or nuclear localization could be observed in the spermatogonia, spermatocytes and spermatids obtained in the heat treated group. These results showed that elevated ambient temperatures induced germ cell apoptosis. In response to heat stress, the expression of Bcl-2 increased and a redistribution of Bax from a cytoplasmic to a perinuclear or nuclear localization. This indicates that Bcl-2 and Bax may be involved in regulation of germ cell apoptosis induced by heat stress in boars.

      PubDate: 2017-03-08T04:14:16Z
      DOI: 10.1016/j.acthis.2016.09.003
  • Comparative study of two models of combined pulmonary fibrosis and
           emphysema in mice
    • Authors: Wan-Guang Zhang; Si-Si Wu; Li He; Qun Yang; Yi-Kuan Feng; Yue-Tao Chen; Guo-Hua Zhen; Yong-Jian Xu; Zhen-Xiang Zhang; Jian-Ping Zhao; Hui-Lan Zhang
      Abstract: Publication date: Available online 21 February 2017
      Source:Acta Histochemica
      Author(s): Wan-Guang Zhang, Si-Si Wu, Li He, Qun Yang, Yi-Kuan Feng, Yue-Tao Chen, Guo-Hua Zhen, Yong-Jian Xu, Zhen-Xiang Zhang, Jian-Ping Zhao, Hui-Lan Zhang
      Combined pulmonary fibrosis and emphysema (CPFE) is an “umbrella term” encompassing emphysema and pulmonary fibrosis, but its pathogenesis is not known. We established two models of CPFE in mice using tracheal instillation with bleomycin (BLM) or murine gammaherpesvirus 68 (MHV-68). Experimental mice were divided randomly into four groups: A (normal control, n=6), B (emphysema, n=6), C (emphysema+MHV-68, n=24), D (emphysema+BLM, n=6). Group C was subdivided into four groups: C1 (sacrificed on day 367, 7 days after tracheal instillation of MHV-68); C2 (day 374; 14days); C3 (day 381; 21days); C4 (day 388; 28days). Conspicuous emphysema and interstitial fibrosis were observed in BLM and MHV-68 CPFE mouse models. However, BLM induced diffuse pulmonary interstitial fibrosis with severely diffuse pulmonary inflammation; MHV-68 induced relatively modest inflammation and fibrosis, and the inflammation and fibrosis were not diffuse, but instead around bronchioles. Inflammation and fibrosis were detectable in the day-7 subgroup and reached a peak in the day-28 subgroup in the emphysema + MHV-68 group. Levels of macrophage chemoattractant protein-1, macrophage inflammatory protein-1α, interleukin-13, and transforming growth factor-β1 in bronchoalveolar lavage fluid were increased significantly in both models. Percentage of apoptotic type-2 lung epithelial cells was significantly higher; however, all four types of cytokine and number of macrophages were significantly lower in the emphysema+MHV-68 group compared with the emphysema +BLM group. The different changes in pathology between BLM and MHV-68 mice models demonstrated different pathology subtypes of CPFE: macrophage infiltration and apoptosis of type-II lung epithelial cells increased with increasing pathology score for pulmonary fibrosis.

      PubDate: 2017-02-23T04:04:46Z
      DOI: 10.1016/j.acthis.2017.01.007
  • Wnt-1 immunodetection in the regenerating tail of lizard suggests it is
           involved in the proliferation and distal growth of the blastema
    • Authors: Lorenzo Alibardi
      Abstract: Publication date: Available online 21 February 2017
      Source:Acta Histochemica
      Author(s): Lorenzo Alibardi
      Lizard tail regeneration depends from growth of the apical tip and autonomous regeneration of a new spinal cord, cartilaginous tube and muscles. The presence of embryonic signaling pathways is likely involved and we have focused on immunolocalization of Wnt1 protein in regenerating tissues, a protein promoting proliferation and tumorigenesis. Western blot indicates some immunoreactive bands in the expected range at 46 and 33kDa in the regenerating tail. Immunolocalization indicates that Wnt1 is prevalently detected in the apical wound epidermis, blastema, and ependyma ampulla of the regenerating tail while it lowers in other tissues of more proximal regions close to the original tail stump. Although a gradient for Wnt1 was not detected, the higher immunofluorescence present in the apical region of the blastema and around the regenerating spinal cord indicates that the protein could be secreted from the apical wound epidermis and the ependyma and might influence cell proliferation in the blastema, the distal-most growing center of the new tail. The present observations suggest the involvement of the Wnt pathway to direct the process of tail regeneration in lizard. The stimulation of proliferation of epidermal and mesenchymal cells in the apical blastema by Wnt proteins remains to be experimentally validated.

      PubDate: 2017-02-23T04:04:46Z
      DOI: 10.1016/j.acthis.2017.01.001
  • Effects of melatonin in rats in the initial third stage of pregnancy
           exposed to sub-lethal doses of herbicides
    • Authors: Lécio Leone de Almeida; Álvaro Aguiar Coelho Teixeira; Anísio Francisco Soares; Franklin Magliano da Cunha; Valdemiro Amaro da Silva; Leucio Duarte Vieira Filho; Valéria Wanderley-Teixeira
      Abstract: Publication date: Available online 13 February 2017
      Source:Acta Histochemica
      Author(s): Lécio Leone de Almeida, Álvaro Aguiar Coelho Teixeira, Anísio Francisco Soares, Franklin Magliano da Cunha, Valdemiro Amaro da Silva, Leucio Duarte Vieira Filho, Valéria Wanderley-Teixeira
      Exposure to the herbicides Paraquat (PQ) and Roundup® may cause cell lesions due to an increase in oxidative stress levels in different biological systems, even in the reproductive system. Objective Evaluate the possible changes in reproductive parameters and hepatic, as well as its prevention by simultaneous application of melatonin. Methods Thirty-five female rats at the age of 3 months were divided into seven groups: three groups exposed to sub-lethal doses of the herbicides PQ (50mg/kg) and Roundup® (500mg/kg) (n=5, G2, G3 and G4); three groups exposed to herbicides and simultaneous treatment with 10mg/kg of Melatonin (n=5, G5, G6 and G7) and control group (n=5, G1) from the first to the seventh day of pregnancy. On the seventh day of pregnancy, the rats were anesthetized and euthanized, followed by laparotomy to remove their reproductive tissues and liver. Body and ovary weights were taken and the number of implantation sites, corpora lutea, preimplantation losses, implantation rates were counted and histopathology of the implantation sites, morphometry of the surface and glandular epithelia of endometrium and hepatic oxidative stress were undertaken. Results The present study shows the decrease in body and ovary weight, decrease in the number of implantation sites, implantation rate, in the total number of corpora lutea and increase of preimplantation percentages were observed when compared to the G1: Fig. 1 and Table 1, (p>0.001 ANOVA/Tukey). The histopathological analysis of the implantation sites showed a disorder of the cytotrophoblast and cell degeneration within the blastocyst cavity in Fig. 4. Morphometry revealed a reduction in surface and glandular epithelia and in the diameter of the endometrial glands (Table 2; p>0.05 ANOVA/Tukey), whereas in liver, serum levels of thiobarbituric acid reactive substances (TBARS) were found to be significantly elevated (Fig. 2; p>0.001; p>0.05 ANOVA/Tukey), and serum level of reduced glutathione (GSH) was significantly lower (Fig. 3; p>0.001 ANOVA/Tukey). However, treatments with melatonin exhibited improvements in reproductive parameters, as well as reduced lesions in the implantation sites (Fig. 4.) and in serum levels TBARS (Fig. 2; p>0.001 ANOVA/Tukey), serum levels GSH (Fig. 3; p>0.001; p>0.05 ANOVA/Tukey). Conclusions These results reveal that melatonin is a protective agent against experimentally induced maternal/embryo toxicity with herbicides and favoring normalization of reproductive parameters and hepatic.

      PubDate: 2017-02-16T04:00:16Z
      DOI: 10.1016/j.acthis.2017.01.003
  • Role of Rho-specific guanine nucleotide dissociation inhibitor α
           regulation in cell migration
    • Authors: Fei Xie; Shuai Shao; Aziz ur Rehman Aziz; Baohong Zhang; Hanqin Wang; Bo Liu
      Abstract: Publication date: Available online 8 February 2017
      Source:Acta Histochemica
      Author(s): Fei Xie, Shuai Shao, Aziz ur Rehman Aziz, Baohong Zhang, Hanqin Wang, Bo Liu
      Cell migration is a vital process for many physiological and pathological events, and Rho GTPases have been confirmed as key factors in its regulation. The most studied negative regulator of Rho GTPases, Rho-specific guanine nucleotide dissociation inhibitor α (RhoGDIα), mediates cell migration through altering the overall expression and spatiotemporal activation of Rho GTPases. The RhoGDIα-Rho GTPases dissociation can be mediated by signal pathways targeting RhoGDIα directly. This review summarizes the research about the regulation of RhoGDIα during cell migration, which can be in a Rho GTPases association independent manner. Non-kinase proteins regulation, phosphorylation, SUMOylation and extracellular environmental factors are classified to discuss their direct signal regulations on RhoGDIα, which provide varied signal pathways for selective activation of Rho GTPases in cell migration.

      PubDate: 2017-02-10T03:56:34Z
      DOI: 10.1016/j.acthis.2017.01.008
  • Effect of dietary zinc on morphological characteristics and apoptosis
           related gene expression in the small intestine of Bama miniature pigs
    • Authors: Xin Zhou; Yansen Li; Zhaojian Li; Yun Cao; Fei Wang; ChunMei Li
      Abstract: Publication date: Available online 4 February 2017
      Source:Acta Histochemica
      Author(s): Xin Zhou, Yansen Li, Zhaojian Li, Yun Cao, Fei Wang, ChunMei Li
      To investigate the effects of dietary zinc (Zn) on small intestinal mucosal epithelium, 6-month-old male Bama miniature pigs were randomly allocated into three groups and treated with three levels of Zn (Control, T1, and T2 diet supplemented with 0, 50, and 1500mg/kg Zn, respectively, as zinc sulfate) for 38days. The samples of small intestine tissues, serum, and feces were collected. The results showed that Zn concentrations of small intestine in the T2 group were higher than those in the control and T1 groups (p< 0.05). In the T2 group, the pharmacological dose of dietary Zn treatment caused marked damage to the small intestinal epithelium. The expression of Bax, cleaved caspase-3, and caspase-8 were increased in the duodenum and the jejunum of the T2 group (p< 0.05). The mRNA transcript levels of BAX, CYCS and CASP3 genes were upregulated in the duodenum and the jejunum of the T2 group. We concluded that a diet with a pharmacological dose of Zn increased the accumulation of Zn and the expression of Bax, cleaved caspase-3, and caspase-8, which might activate the apoptosis and lead to the marked injury of porcine small intestinal epithelium.
      Graphical abstract image

      PubDate: 2017-02-10T03:56:34Z
      DOI: 10.1016/j.acthis.2017.01.006
  • Forkhead box-P3+ regulatory T cells and toll-like receptor 2 co-expression
           in oral squamous cell carcinoma
    • Authors: H.M. Hussaini; V.P.B. Parachuru; G.J. Seymour; A.M. Rich
      Abstract: Publication date: Available online 4 February 2017
      Source:Acta Histochemica
      Author(s): H.M. Hussaini, V.P.B. Parachuru, G.J. Seymour, A.M. Rich
      Background The function of forkhead box-P3 (FoxP3) regulatory T cells (Treg) and toll-like receptor (TLR)2 protein in the oral cancer microenvironment is not fully understood, but evidence from other malignancies suggests it is likely they are involved with tumour development and progression. The aim of this study was to investigate the distribution of FoxP3+cells, TLR2+ cells and double-labelled FoxP3+TLR2+ immune cells in oral squamous cell carcinoma (OSCC), using immunohistochemistry (IHC) and immunofluorescence (IF). Methods 25 archival cases of OSCC were immunostained with anti-FoxP3 and anti-TLR2 antibodies. Inflamed hyperplastic oral mucosal tissues were used as controls. The proportion of single-labelled, double-labelled and negative cells was determined. Results A higher frequency of double-labelled FoxP3+TLR2+ Tregs was observed within the immune cells of OSCC compared to inflamed controls using IHC (p<0.05). Cell-to-cell contact between single-stained TLR2+ cells and FoxP3+ cells was noted. Double IF studies validated demonstration of co-expression of FoxP3+/TLR2+ immune cells in OSCC. Conclusion The presence of FoxP3+TLR2+ cells within the OSCC microenvironment may represent a dendritic cell-dependent pathway capable of inhibiting Treg suppressive activity, potentially enhancing the anti-tumour response. Modulation of TLR2-Treg interactions should be further explored to determine if they have a role in the therapeutic management of OSCC.

      PubDate: 2017-02-10T03:56:34Z
      DOI: 10.1016/j.acthis.2016.12.005
  • Immunoreactivity pattern of calretinin in the developing human cerebellar
    • Authors: Valeria Pibiri; Clara Gerosa; Laura Vinci; Gavino Faa; Rossano Ambu
      Abstract: Publication date: Available online 4 February 2017
      Source:Acta Histochemica
      Author(s): Valeria Pibiri, Clara Gerosa, Laura Vinci, Gavino Faa, Rossano Ambu
      The immunohistochemical expression of the calcium-binding protein calretinin during human cerebellar development has been investigated in this study. Human cerebellum samples, obtained from 7 fetuses and newborns ranging from 11 to 38 weeks of gestation, were 10% formalin-fixed, routinely processed and paraffin-embedded. 3μm-tick sections were immunostained with an anti-calretinin antibody. Our study evidenced a different immunoreactivity for calretinin in Purkinje cells and in several cerebellar interneurons at different intrauterine developmental stages. Whereas at 11 weeks of gestation calretinin immunoreactivity was not detected in the developing cerebellum, from the 18th to the 24th week, calretinin expression was found in Purkinje cells migrating from the ventricular neuroepithelium and in migrating cerebellar interneurons. From the 30th to the 38th week, calretinin was expressed by most of Purkinje cells and by migrating cerebellar interneurons. Furthermore, granule cells in the internal granular layer were also immunoreactive for calretinin. Our data show that calretinin, other than for developing Purkinje cells, is a useful marker also for migrating cerebellar interneurons and for some neuronal elements related to the granular layer. Moreover, given the critical role of calcium in a great variety of neuronal processes in the central nervous system, our findings suggest that calretinin may play a pivotal role in the regulation of neuronal excitability during intrauterine cerebellar development.

      PubDate: 2017-02-10T03:56:34Z
      DOI: 10.1016/j.acthis.2017.01.005
  • Ribosome biogenesis and cancer
    • Authors: Massimo Derenzini; Lorenzo Montanaro; Davide Trerè
      Abstract: Publication date: Available online 3 February 2017
      Source:Acta Histochemica
      Author(s): Massimo Derenzini, Lorenzo Montanaro, Davide Trerè
      There is growing evidence indicating that the human pathological conditions characterized by an up-regulated ribosome biogenesis are at an increased risk of cancer onset. At the basis of this relationship is the close interconnection between the ribosome biogenesis and cell proliferation. Cell proliferation-stimulating factors also stimulate ribosome production, while the ribosome biogenesis rate controls the cell cycle progression. The major tumour suppressor, the p53 protein, plays an important balancing role between the ribosome biogenesis rate and the cell progression through the cell cycle phases. The perturbation of ribosome biogenesis stabilizes and activates p53, with a consequent cell cycle arrest and/or apoptotic cell death, whereas an up-regulated ribosome production down-regulates p53 expression and activity, thus facilitating neoplastic transformation. In the present review we describe the interconnection between ribosome biogenesis and cell proliferation, while highlighting the mechanisms by which quantitative changes in ribosome biogenesis may induce cancer.

      PubDate: 2017-02-04T03:52:24Z
      DOI: 10.1016/j.acthis.2017.01.009
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