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Publisher: Elsevier   (Total: 3031 journals)

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Showing 1 - 200 of 3031 Journals sorted alphabetically
AASRI Procedia     Open Access   (Followers: 15)
Academic Pediatrics     Hybrid Journal   (Followers: 20, SJR: 1.402, h-index: 51)
Academic Radiology     Hybrid Journal   (Followers: 16, SJR: 1.008, h-index: 75)
Accident Analysis & Prevention     Partially Free   (Followers: 79, SJR: 1.109, h-index: 94)
Accounting Forum     Hybrid Journal   (Followers: 22, SJR: 0.612, h-index: 27)
Accounting, Organizations and Society     Hybrid Journal   (Followers: 27, SJR: 2.515, h-index: 90)
Achievements in the Life Sciences     Open Access   (Followers: 4)
Acta Anaesthesiologica Taiwanica     Open Access   (Followers: 5, SJR: 0.338, h-index: 19)
Acta Astronautica     Hybrid Journal   (Followers: 302, SJR: 0.726, h-index: 43)
Acta Automatica Sinica     Full-text available via subscription   (Followers: 3)
Acta Biomaterialia     Hybrid Journal   (Followers: 25, SJR: 2.02, h-index: 104)
Acta Colombiana de Cuidado Intensivo     Full-text available via subscription  
Acta de Investigación Psicológica     Open Access   (Followers: 2)
Acta Ecologica Sinica     Open Access   (Followers: 8, SJR: 0.172, h-index: 29)
Acta Haematologica Polonica     Free   (SJR: 0.123, h-index: 8)
Acta Histochemica     Hybrid Journal   (Followers: 3, SJR: 0.604, h-index: 38)
Acta Materialia     Hybrid Journal   (Followers: 195, SJR: 3.683, h-index: 202)
Acta Mathematica Scientia     Full-text available via subscription   (Followers: 5, SJR: 0.615, h-index: 21)
Acta Mechanica Solida Sinica     Full-text available via subscription   (Followers: 9, SJR: 0.442, h-index: 21)
Acta Oecologica     Hybrid Journal   (Followers: 9, SJR: 0.915, h-index: 53)
Acta Otorrinolaringologica (English Edition)     Full-text available via subscription   (Followers: 1)
Acta Otorrinolaringológica Española     Full-text available via subscription   (Followers: 3, SJR: 0.311, h-index: 16)
Acta Pharmaceutica Sinica B     Open Access   (Followers: 2)
Acta Poética     Open Access   (Followers: 4)
Acta Psychologica     Hybrid Journal   (Followers: 21, SJR: 1.365, h-index: 73)
Acta Sociológica     Open Access  
Acta Tropica     Hybrid Journal   (Followers: 5, SJR: 1.059, h-index: 77)
Acta Urológica Portuguesa     Open Access  
Actas Dermo-Sifiliograficas     Full-text available via subscription   (Followers: 4)
Actas Dermo-Sifiliográficas (English Edition)     Full-text available via subscription   (Followers: 3)
Actas Urológicas Españolas     Full-text available via subscription   (Followers: 3, SJR: 0.383, h-index: 19)
Actas Urológicas Españolas (English Edition)     Full-text available via subscription   (Followers: 2)
Actualites Pharmaceutiques     Full-text available via subscription   (Followers: 5, SJR: 0.141, h-index: 3)
Actualites Pharmaceutiques Hospitalieres     Full-text available via subscription   (Followers: 4, SJR: 0.112, h-index: 2)
Acupuncture and Related Therapies     Hybrid Journal   (Followers: 4)
Ad Hoc Networks     Hybrid Journal   (Followers: 11, SJR: 0.967, h-index: 57)
Addictive Behaviors     Hybrid Journal   (Followers: 15, SJR: 1.514, h-index: 92)
Addictive Behaviors Reports     Open Access   (Followers: 5)
Additive Manufacturing     Hybrid Journal   (Followers: 7, SJR: 1.039, h-index: 5)
Additives for Polymers     Full-text available via subscription   (Followers: 20)
Advanced Drug Delivery Reviews     Hybrid Journal   (Followers: 119, SJR: 5.2, h-index: 222)
Advanced Engineering Informatics     Hybrid Journal   (Followers: 11, SJR: 1.265, h-index: 53)
Advanced Powder Technology     Hybrid Journal   (Followers: 16, SJR: 0.739, h-index: 33)
Advances in Accounting     Hybrid Journal   (Followers: 8, SJR: 0.299, h-index: 15)
Advances in Agronomy     Full-text available via subscription   (Followers: 15, SJR: 2.071, h-index: 82)
Advances in Anesthesia     Full-text available via subscription   (Followers: 24, SJR: 0.169, h-index: 4)
Advances in Antiviral Drug Design     Full-text available via subscription   (Followers: 3)
Advances in Applied Mathematics     Full-text available via subscription   (Followers: 6, SJR: 1.054, h-index: 35)
Advances in Applied Mechanics     Full-text available via subscription   (Followers: 10, SJR: 0.801, h-index: 26)
Advances in Applied Microbiology     Full-text available via subscription   (Followers: 21, SJR: 1.286, h-index: 49)
Advances In Atomic, Molecular, and Optical Physics     Full-text available via subscription   (Followers: 16, SJR: 3.31, h-index: 42)
Advances in Biological Regulation     Hybrid Journal   (Followers: 4, SJR: 2.277, h-index: 43)
Advances in Botanical Research     Full-text available via subscription   (Followers: 3, SJR: 0.619, h-index: 48)
Advances in Cancer Research     Full-text available via subscription   (Followers: 26, SJR: 2.215, h-index: 78)
Advances in Carbohydrate Chemistry and Biochemistry     Full-text available via subscription   (Followers: 9, SJR: 0.9, h-index: 30)
Advances in Catalysis     Full-text available via subscription   (Followers: 5, SJR: 2.139, h-index: 42)
Advances in Cellular and Molecular Biology of Membranes and Organelles     Full-text available via subscription   (Followers: 12)
Advances in Chemical Engineering     Full-text available via subscription   (Followers: 24, SJR: 0.183, h-index: 23)
Advances in Child Development and Behavior     Full-text available via subscription   (Followers: 10, SJR: 0.665, h-index: 29)
Advances in Chronic Kidney Disease     Full-text available via subscription   (Followers: 8, SJR: 1.268, h-index: 45)
Advances in Clinical Chemistry     Full-text available via subscription   (Followers: 28, SJR: 0.938, h-index: 33)
Advances in Colloid and Interface Science     Full-text available via subscription   (Followers: 18, SJR: 2.314, h-index: 130)
Advances in Computers     Full-text available via subscription   (Followers: 16, SJR: 0.223, h-index: 22)
Advances in Developmental Biology     Full-text available via subscription   (Followers: 11)
Advances in Digestive Medicine     Open Access   (Followers: 4)
Advances in DNA Sequence-Specific Agents     Full-text available via subscription   (Followers: 5)
Advances in Drug Research     Full-text available via subscription   (Followers: 22)
Advances in Ecological Research     Full-text available via subscription   (Followers: 39, SJR: 3.25, h-index: 43)
Advances in Engineering Software     Hybrid Journal   (Followers: 25, SJR: 0.486, h-index: 10)
Advances in Experimental Biology     Full-text available via subscription   (Followers: 7)
Advances in Experimental Social Psychology     Full-text available via subscription   (Followers: 38, SJR: 5.465, h-index: 64)
Advances in Exploration Geophysics     Full-text available via subscription   (Followers: 3)
Advances in Fluorine Science     Full-text available via subscription   (Followers: 8)
Advances in Food and Nutrition Research     Full-text available via subscription   (Followers: 41, SJR: 0.674, h-index: 38)
Advances in Fuel Cells     Full-text available via subscription   (Followers: 14)
Advances in Genetics     Full-text available via subscription   (Followers: 15, SJR: 2.558, h-index: 54)
Advances in Genome Biology     Full-text available via subscription   (Followers: 11)
Advances in Geophysics     Full-text available via subscription   (Followers: 6, SJR: 2.325, h-index: 20)
Advances in Heat Transfer     Full-text available via subscription   (Followers: 18, SJR: 0.906, h-index: 24)
Advances in Heterocyclic Chemistry     Full-text available via subscription   (Followers: 8, SJR: 0.497, h-index: 31)
Advances in Human Factors/Ergonomics     Full-text available via subscription   (Followers: 22)
Advances in Imaging and Electron Physics     Full-text available via subscription   (Followers: 2, SJR: 0.396, h-index: 27)
Advances in Immunology     Full-text available via subscription   (Followers: 33, SJR: 4.152, h-index: 85)
Advances in Inorganic Chemistry     Full-text available via subscription   (Followers: 9, SJR: 1.132, h-index: 42)
Advances in Insect Physiology     Full-text available via subscription   (Followers: 3, SJR: 1.274, h-index: 27)
Advances in Integrative Medicine     Hybrid Journal   (Followers: 4)
Advances in Intl. Accounting     Full-text available via subscription   (Followers: 4)
Advances in Life Course Research     Hybrid Journal   (Followers: 7, SJR: 0.764, h-index: 15)
Advances in Lipobiology     Full-text available via subscription   (Followers: 1)
Advances in Magnetic and Optical Resonance     Full-text available via subscription   (Followers: 8)
Advances in Marine Biology     Full-text available via subscription   (Followers: 16, SJR: 1.645, h-index: 45)
Advances in Mathematics     Full-text available via subscription   (Followers: 10, SJR: 3.261, h-index: 65)
Advances in Medical Sciences     Hybrid Journal   (Followers: 5, SJR: 0.489, h-index: 25)
Advances in Medicinal Chemistry     Full-text available via subscription   (Followers: 5)
Advances in Microbial Physiology     Full-text available via subscription   (Followers: 4, SJR: 1.44, h-index: 51)
Advances in Molecular and Cell Biology     Full-text available via subscription   (Followers: 21)
Advances in Molecular and Cellular Endocrinology     Full-text available via subscription   (Followers: 10)
Advances in Molecular Toxicology     Full-text available via subscription   (Followers: 6, SJR: 0.324, h-index: 8)
Advances in Nanoporous Materials     Full-text available via subscription   (Followers: 3)
Advances in Oncobiology     Full-text available via subscription   (Followers: 3)
Advances in Organometallic Chemistry     Full-text available via subscription   (Followers: 15, SJR: 2.885, h-index: 45)
Advances in Parallel Computing     Full-text available via subscription   (Followers: 7, SJR: 0.148, h-index: 11)
Advances in Parasitology     Full-text available via subscription   (Followers: 7, SJR: 2.37, h-index: 73)
Advances in Pediatrics     Full-text available via subscription   (Followers: 20, SJR: 0.4, h-index: 28)
Advances in Pharmaceutical Sciences     Full-text available via subscription   (Followers: 14)
Advances in Pharmacology     Full-text available via subscription   (Followers: 13, SJR: 1.718, h-index: 58)
Advances in Physical Organic Chemistry     Full-text available via subscription   (Followers: 7, SJR: 0.384, h-index: 26)
Advances in Phytomedicine     Full-text available via subscription  
Advances in Planar Lipid Bilayers and Liposomes     Full-text available via subscription   (Followers: 3, SJR: 0.248, h-index: 11)
Advances in Plant Biochemistry and Molecular Biology     Full-text available via subscription   (Followers: 8)
Advances in Plant Pathology     Full-text available via subscription   (Followers: 5)
Advances in Porous Media     Full-text available via subscription   (Followers: 4)
Advances in Protein Chemistry     Full-text available via subscription   (Followers: 18)
Advances in Protein Chemistry and Structural Biology     Full-text available via subscription   (Followers: 17, SJR: 1.5, h-index: 62)
Advances in Psychology     Full-text available via subscription   (Followers: 56)
Advances in Quantum Chemistry     Full-text available via subscription   (Followers: 5, SJR: 0.478, h-index: 32)
Advances in Radiation Oncology     Open Access  
Advances in Small Animal Medicine and Surgery     Hybrid Journal   (Followers: 1, SJR: 0.1, h-index: 2)
Advances in Space Research     Full-text available via subscription   (Followers: 332, SJR: 0.606, h-index: 65)
Advances in Structural Biology     Full-text available via subscription   (Followers: 7)
Advances in Surgery     Full-text available via subscription   (Followers: 6, SJR: 0.823, h-index: 27)
Advances in the Study of Behavior     Full-text available via subscription   (Followers: 28, SJR: 1.321, h-index: 56)
Advances in Veterinary Medicine     Full-text available via subscription   (Followers: 14)
Advances in Veterinary Science and Comparative Medicine     Full-text available via subscription   (Followers: 12)
Advances in Virus Research     Full-text available via subscription   (Followers: 5, SJR: 1.878, h-index: 68)
Advances in Water Resources     Hybrid Journal   (Followers: 42, SJR: 2.408, h-index: 94)
Aeolian Research     Hybrid Journal   (Followers: 5, SJR: 0.973, h-index: 22)
Aerospace Science and Technology     Hybrid Journal   (Followers: 303, SJR: 0.816, h-index: 49)
AEU - Intl. J. of Electronics and Communications     Hybrid Journal   (Followers: 8, SJR: 0.318, h-index: 36)
African J. of Emergency Medicine     Open Access   (Followers: 4, SJR: 0.344, h-index: 6)
Ageing Research Reviews     Hybrid Journal   (Followers: 7, SJR: 3.289, h-index: 78)
Aggression and Violent Behavior     Hybrid Journal   (Followers: 389, SJR: 1.385, h-index: 72)
Agri Gene     Hybrid Journal  
Agricultural and Forest Meteorology     Hybrid Journal   (Followers: 15, SJR: 2.18, h-index: 116)
Agricultural Systems     Hybrid Journal   (Followers: 29, SJR: 1.275, h-index: 74)
Agricultural Water Management     Hybrid Journal   (Followers: 36, SJR: 1.546, h-index: 79)
Agriculture and Agricultural Science Procedia     Open Access  
Agriculture and Natural Resources     Open Access   (Followers: 1)
Agriculture, Ecosystems & Environment     Hybrid Journal   (Followers: 48, SJR: 1.879, h-index: 120)
Ain Shams Engineering J.     Open Access   (Followers: 5, SJR: 0.434, h-index: 14)
Air Medical J.     Hybrid Journal   (Followers: 3, SJR: 0.234, h-index: 18)
AKCE Intl. J. of Graphs and Combinatorics     Open Access   (SJR: 0.285, h-index: 3)
Alcohol     Hybrid Journal   (Followers: 9, SJR: 0.922, h-index: 66)
Alcoholism and Drug Addiction     Open Access   (Followers: 5)
Alergologia Polska : Polish J. of Allergology     Full-text available via subscription   (Followers: 1)
Alexandria Engineering J.     Open Access   (Followers: 1, SJR: 0.436, h-index: 12)
Alexandria J. of Medicine     Open Access  
Algal Research     Partially Free   (Followers: 7, SJR: 2.05, h-index: 20)
Alkaloids: Chemical and Biological Perspectives     Full-text available via subscription   (Followers: 3)
Allergologia et Immunopathologia     Full-text available via subscription   (Followers: 1, SJR: 0.46, h-index: 29)
Allergology Intl.     Open Access   (Followers: 5, SJR: 0.776, h-index: 35)
ALTER - European J. of Disability Research / Revue Européenne de Recherche sur le Handicap     Full-text available via subscription   (Followers: 6, SJR: 0.158, h-index: 9)
Alzheimer's & Dementia     Hybrid Journal   (Followers: 45, SJR: 4.289, h-index: 64)
Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring     Open Access   (Followers: 5)
Alzheimer's & Dementia: Translational Research & Clinical Interventions     Open Access   (Followers: 3)
American Heart J.     Hybrid Journal   (Followers: 45, SJR: 3.157, h-index: 153)
American J. of Cardiology     Hybrid Journal   (Followers: 47, SJR: 2.063, h-index: 186)
American J. of Emergency Medicine     Hybrid Journal   (Followers: 34, SJR: 0.574, h-index: 65)
American J. of Geriatric Pharmacotherapy     Full-text available via subscription   (Followers: 6, SJR: 1.091, h-index: 45)
American J. of Geriatric Psychiatry     Hybrid Journal   (Followers: 14, SJR: 1.653, h-index: 93)
American J. of Human Genetics     Hybrid Journal   (Followers: 32, SJR: 8.769, h-index: 256)
American J. of Infection Control     Hybrid Journal   (Followers: 25, SJR: 1.259, h-index: 81)
American J. of Kidney Diseases     Hybrid Journal   (Followers: 31, SJR: 2.313, h-index: 172)
American J. of Medicine     Hybrid Journal   (Followers: 48, SJR: 2.023, h-index: 189)
American J. of Medicine Supplements     Full-text available via subscription   (Followers: 3)
American J. of Obstetrics and Gynecology     Hybrid Journal   (Followers: 173, SJR: 2.255, h-index: 171)
American J. of Ophthalmology     Hybrid Journal   (Followers: 51, SJR: 2.803, h-index: 148)
American J. of Ophthalmology Case Reports     Open Access   (Followers: 2)
American J. of Orthodontics and Dentofacial Orthopedics     Full-text available via subscription   (Followers: 6, SJR: 1.249, h-index: 88)
American J. of Otolaryngology     Hybrid Journal   (Followers: 22, SJR: 0.59, h-index: 45)
American J. of Pathology     Hybrid Journal   (Followers: 23, SJR: 2.653, h-index: 228)
American J. of Preventive Medicine     Hybrid Journal   (Followers: 21, SJR: 2.764, h-index: 154)
American J. of Surgery     Hybrid Journal   (Followers: 32, SJR: 1.286, h-index: 125)
American J. of the Medical Sciences     Hybrid Journal   (Followers: 13, SJR: 0.653, h-index: 70)
Ampersand : An Intl. J. of General and Applied Linguistics     Open Access   (Followers: 5)
Anaerobe     Hybrid Journal   (Followers: 4, SJR: 1.066, h-index: 51)
Anaesthesia & Intensive Care Medicine     Full-text available via subscription   (Followers: 52, SJR: 0.124, h-index: 9)
Anaesthesia Critical Care & Pain Medicine     Full-text available via subscription   (Followers: 3)
Anales de Cirugia Vascular     Full-text available via subscription  
Anales de Pediatría     Full-text available via subscription   (Followers: 2, SJR: 0.209, h-index: 27)
Anales de Pediatría (English Edition)     Full-text available via subscription  
Anales de Pediatría Continuada     Full-text available via subscription   (SJR: 0.104, h-index: 3)
Analytic Methods in Accident Research     Hybrid Journal   (Followers: 2, SJR: 2.577, h-index: 7)
Analytica Chimica Acta     Hybrid Journal   (Followers: 38, SJR: 1.548, h-index: 152)
Analytical Biochemistry     Hybrid Journal   (Followers: 152, SJR: 0.725, h-index: 154)
Analytical Chemistry Research     Open Access   (Followers: 7, SJR: 0.18, h-index: 2)
Analytical Spectroscopy Library     Full-text available via subscription   (Followers: 10)
Anesthésie & Réanimation     Full-text available via subscription  
Anesthesiology Clinics     Full-text available via subscription   (Followers: 21, SJR: 0.421, h-index: 40)
Angiología     Full-text available via subscription   (SJR: 0.124, h-index: 9)
Angiologia e Cirurgia Vascular     Open Access  
Animal Behaviour     Hybrid Journal   (Followers: 141, SJR: 1.907, h-index: 126)
Animal Feed Science and Technology     Hybrid Journal   (Followers: 5, SJR: 1.151, h-index: 83)
Animal Reproduction Science     Hybrid Journal   (Followers: 5, SJR: 0.711, h-index: 78)
Annales d'Endocrinologie     Full-text available via subscription   (SJR: 0.394, h-index: 30)
Annales d'Urologie     Full-text available via subscription  
Annales de Cardiologie et d'Angéiologie     Full-text available via subscription   (SJR: 0.177, h-index: 13)
Annales de Chirurgie de la Main et du Membre Supérieur     Full-text available via subscription  
Annales de Chirurgie Plastique Esthétique     Full-text available via subscription   (Followers: 2, SJR: 0.354, h-index: 22)
Annales de Chirurgie Vasculaire     Full-text available via subscription   (Followers: 1)

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Journal Cover Advances in Medical Sciences
  [SJR: 0.489]   [H-I: 25]   [5 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 1896-1126 - ISSN (Online) 1898-4002
   Published by Elsevier Homepage  [3031 journals]
  • Cells of the innate and adaptive immunity and their interactions in
           inflammatory bowel disease
    • Authors: Zbigniew Kmieć; Marta Cyman; Tomasz Jerzy Ślebioda
      Pages: 1 - 16
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Zbigniew Kmieć, Marta Cyman, Tomasz Jerzy Ślebioda
      Inflammatory bowel disease (IBD) is a group of chronic inflammatory conditions of the gastrointestinal tract that includes two major phenotypes, Crohn's disease and ulcerative colitis that are characterized by different clinical features and different course of the immune response. The exact aetiology of IBD still remains unknown, although it is thought that the diseases result from an excessive immune response directed against microbial or environmentally derived antigens which can be triggered by the disruption of the intestinal epithelial barrier integrity. In this review we present immune mechanisms and interactions between cells of the immune system and tissue environment that contribute to the development and progression of IBD in humans. Since dysregulation of the intestinal immune response is a hallmark of chronic inflammatory conditions, we characterize cells of the innate and adaptive immunity involved in the pathogenesis of IBD and their cross-talks. We describe various subclasses of recently discovered innate lymphoid cells, as well as dendritic cells, macrophages and T cells, including Th17, Th22 and T regulatory cells, present in the intestinal lamina propria and cytokine-mediated regulation of the immune response in IBD, highlighting the role of IL-22 and IL-17A/IL-23 axis. Insights into novel therapeutic modalities targeting certain elements of the immune pathways important for the pathogenesis of IBD have been also shortly presented.

      PubDate: 2017-01-27T21:26:59Z
      DOI: 10.1016/j.advms.2016.09.001
  • New insights into the pathogenesis and treatment of irritable bowel
    • Authors: Joanna Oświęcimska; Agnieszka Szymlak; Wojciech Roczniak; Katarzyna Girczys-Połedniok; Jarosław Kwiecień
      Pages: 17 - 30
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Joanna Oświęcimska, Agnieszka Szymlak, Wojciech Roczniak, Katarzyna Girczys-Połedniok, Jarosław Kwiecień
      Irritable bowel syndrome (IBS) is one of the most common functional gastrointestinal disorders (FGID), characterized by abdominal pain and a change in stool form that cannot be explained by structural abnormalities. Its prevalence ranges from 9 to 23% of the worldwide population. The pathophysiology of IBS is diverse and not well understood. Biopsychosocial concept assumes that the disease is a product of psychosocial factors and altered at multiple levels of gut physiology interactions. Some aetiological factors have been identified, yet. One of the most important is the disruption of brain-gut mutual communication that leads to visceral hypersensitivity. Also genetic and epigenetic factors are involved. Chronic stress may predispose to IBS as well as exacerbate its symptoms. Both quantitative and qualitative disorders of the gut microbiota are observed. There is also a relationship between the IBS symptoms and the intake of a specific type of food products. In the diarrhoea type of IBS the role of previous gastrointestinal infection is demonstrated. Recent studies have suggested that visceral hypersensitivity in patients with IBS may be secondary to the activation of the immune cells and low-grade inflammation. Clinical symptoms of IBS include abdominal pain and change in bowel habits as well as somatic and psychiatric comorbidities. IBS is diagnosed on the basis of Rome Diagnostic Criteria. Recently, their newest version (Rome IV) has been presented. The aim of this review is to summarize the past decade progress in IBS diagnosis, main pathophysiological aspects and therapeutic management strategy.

      PubDate: 2017-01-27T21:26:59Z
      DOI: 10.1016/j.advms.2016.11.001
  • Amyloidosis: A cancer-derived paraproteinemia and kidney involvement
    • Authors: Jolanta Małyszko; Klaudia Kozłowska; Jacek Stanisław Małyszko
      Pages: 31 - 38
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Jolanta Małyszko, Klaudia Kozłowska, Jacek Stanisław Małyszko
      Amyloidosis is the general term describing the extracellular tissue deposition of fibrils composed of low molecular weight subunits of a variety of proteins. There are multiple different human protein precursors of amyloid fibrils. Amyloid deposits are stained using Congo Red and show typical apple-green birefringence in polarized microscopy. Nowadays, a novel technique LMD/MS technique or laser microdissection combined with mass spectrometry help to diagnose amyloidosis. Amyloidosis of the kidney is typically classified as being either one of two types: AL or AA. Less common is the hereditary amyloidosis. Clinical manifestations are usually determined by the type of precursor protein, the tissue distribution, and the amount of amyloid deposition. Renal manifestation is usually present as asymptomatic proteinuria or clinically apparent nephrotic syndrome. In some patients clinical presentation include impaired kidney function with no or mild proteinuria. Patients with renal amyloidosis who progress to end-stage renal disease (ESRD) can be treated with either dialysis or renal transplantation. Diagnosis of amyloidosis is prerequisite to consider treatment options to avoid unnecessary chemotherapy. Treatment of amyloidosis is aimed at decreasing the precursors of fibrillary proteins and/or decrease in synthesis/deposition of amyloid fibrils. It depends upon the type of amyloidosis and cause of excess fibril production.

      PubDate: 2017-02-03T21:55:38Z
      DOI: 10.1016/j.advms.2016.06.004
  • Does the OPG/RANKL system contribute to the bone-vascular axis in chronic
           kidney disease? A systematic review
    • Authors: Beata Znorko; Ewa Oksztulska-Kolanek; Małgorzata Michałowska; Tomasz Kamiński; Krystyna Pawlak
      Pages: 52 - 64
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Beata Znorko, Ewa Oksztulska-Kolanek, Małgorzata Michałowska, Tomasz Kamiński, Krystyna Pawlak
      Vascular calcification (VC) is highly prevalent in patients with chronic kidney disease (CKD) and is strongly associated with cardiovascular mortality and morbidity. Accumulating evidence over the past decade has challenged the hypothesis of close interaction between bone and VC what raises the possibility of a common underlying pathophysiological mechanism. Lately, bone regulatory proteins such as: osteoprotegerin (OPG) and Receptor Activator for Nuclear Factor κB Ligand (RANKL) has attracted attention of researchers as a possible key mediators of bone-vascular calcification imbalance. The literature search was carried out using the MEDLINE/PubMed database and a combination of keywords and MeSH terms, and only papers published since January 2005 to July 2016 were selected. The search resulted in 562 potential articles. After selection according to the eligibility criteria, 107 studies fulfilled were included (102 full texts and 5 was case reports). OPG and RANKL plays essential role in the regulation of bone metabolism and may be regarded as a possible link between VC, bone and mineral metabolism in CKD patients. Further studies are required to determine the diagnostic significance of these proteins in evaluation of progression and severity of VC process in CKD patients. Finally, the efficacy and safety, especially in regard to VC, of anti-RANKL therapy in CKD patients requires well-designed prospective, randomized trials.

      PubDate: 2017-02-10T01:43:18Z
      DOI: 10.1016/j.advms.2016.08.001
  • Immunohistochemical features of multifocal and multicentric lobular breast
    • Authors: Ivan R. Ilić; Aleksandar Petrović; Vesna V. Živković; Pavle J. Randjelović; Nikola M. Stojanović; Niko S. Radulović; Dušica Randjelović; Ratko S. Ilić
      Pages: 78 - 82
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Ivan R. Ilić, Aleksandar Petrović, Vesna V. Živković, Pavle J. Randjelović, Nikola M. Stojanović, Niko S. Radulović, Dušica Randjelović, Ratko S. Ilić
      Purpose Patient age, tumor size, axillary lymph node status, expression of estrogen, progesterone and HER2 receptors, as well as the histological grade, are widely accepted prognostic and predictive parameters in breast carcinoma. In invasive lobular carcinoma (ILC), the prognostic factors, such as nodal status and tumor size are believed to be the most valuable ones. The aim of this was set to determine the possible linkage between MFity/MCity of ILC and immunohistochemical predictive (ER, PR, HER2) and prognostic parameters (BRCA1, p53, E-cadherin, Ki-67). Material/methods Out of 2486 cases diagnosed with BC from south-eastern Serbia, the presence ILC was noted in 334 cases. Immunohistochemical characterization of predictive and prognostic parameters in ILC was done. Results The occurrence of multifocal (MFC) and multicentric carcinoma (MCC) was observed in 18.9% of ILC. Bilateral (BL) BCs were found to be statistically significantly more frequent in younger women, compared to those with MFC, MCC or nonMFC/MCC/BL. No significant correlation was found between MFC/MCC and the presence of axillary lymph node metastases, and expression of immunohistochemical predictive and prognostic parameters. Based on literature data and the findings from the current work, MFity/MCtiy might represent negative morphologic prognostic parameters in ILC. Conclusions In 334 cases analyzed, no statistically significant correlations were observed between MF/MC and the expression of immunohistochemical predictive and prognostic parameters.

      PubDate: 2017-02-16T03:36:08Z
      DOI: 10.1016/j.advms.2016.07.003
  • Overexpression of ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) in
           serum of children after thermal injury
    • Authors: Ewa Matuszczak; Marzena Tylicka; Wojciech Dębek; Anna Sankiewicz; Ewa Gorodkiewicz; Adam Hermanowicz
      Pages: 83 - 86
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Ewa Matuszczak, Marzena Tylicka, Wojciech Dębek, Anna Sankiewicz, Ewa Gorodkiewicz, Adam Hermanowicz
      Purpose The study aims to determinate concentrations of ubiquitin C-terminal hydrolase 1 (UCHL1), which hydrolyzes amino acids from ubiquitin and cleave di-ubiquitins, in serum of children after thermal injury. Patients/methods 42 children scalded by hot water, managed at the Department of Pediatric Surgery, with burns in 4–20% TBSA were included into the study (age 9 months up to 14 years, mean age 2.5±1 years). Blood plasma UCHL1 concentration was assessed in 2–6h, 12–16h, 3d, 5d, and 7d after injury using surface plasmon resonance imaging biosensor. 18 healthy subjects admitted for planned surgeries served as controls. Results The UCHL1 concentration in the blood plasma of patients with thermal injuries reached its peak 12–16h after thermal injury and slowly decreased over time, and still did not reach the normal range on the 7th day after thermal injury. Mean concentrations of UCHL1 after thermal injury were above the range measured in controls (0.12ng/ml): 2–6h after injury – 5.59ng/dl, 12–16h after injury – 9.16ng/dl, 3 days after injury – 6.94ng/dl, 5 days after 5.41ng/dl, 7 days after injury – 4.09ng/dl. Conclusions We observed sudden increase in the concentration of UCHL1 2–16h after thermal injury with the slow decrease in the UCHL1 concentration over the time. UCHL1 concentration was proportional to the severity of the burn. Further studies are needed to determine the mechanisms by which UCHL1 contributes to metabolic response following thermal injury.

      PubDate: 2017-02-16T03:36:08Z
      DOI: 10.1016/j.advms.2016.07.004
  • Tissue plasminogen activator (t-PA) and plasminogen activator inhibitor
           type 1 (PAI-1) in diabetic foot syndrome
    • Authors: Arleta Kulwas; Beata Lisewska; Wiesław Jundziłł; Barbara Ruszkowska; Wanda Drewniak; Zofia Ruprecht; Grażyna Gadomska; Danuta Rość
      Pages: 87 - 91
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Arleta Kulwas, Beata Lisewska, Wiesław Jundziłł, Barbara Ruszkowska, Wanda Drewniak, Zofia Ruprecht, Grażyna Gadomska, Danuta Rość
      Purpose The aim was to evaluate tissue plasminogen activator (tPA) and plasminogen activator inhibitor type 1 (PAI-1) concentration using enzyme linked immunosorbent assay method (ELISA) in diabetic foot syndrome (DFS) as compared to a group of healthy people and patients with diabetes mellitus without symptomatic vascular complications (DM2T). Material/methods Venous blood samples were collected from 90 patients with type 2 diabetes mellitus (30 – DM2T; 60 – DFS). Age-matched controls were also included (n =30). tPA and PAI-1 plasma levels were measured by enzyme-linked immunosorbent assay (ELISA). Results We found a significantly lower concentration of tPA:Ag in patients with DFS in comparison to the DM2T group; tPA concentrations were significantly higher in DM2T as compared to the control group. We observed significantly lower concentration of PAI-1:Ag in DF patients treated for hypertension as compared to patients without hypertension. The tPA:Ag and PAI-1:Ag concentration analysis in DFS depending on age, gender and BMI did not show any significant differences. Conclusions A lower concentration of tPA in patients with DFS may be associated with damage to the endothelial cells, especially in the microvasculature, and the sympathetic nervous system.

      PubDate: 2017-02-16T03:36:08Z
      DOI: 10.1016/j.advms.2016.07.007
  • Measuring of quality of life in autoimmune blistering disorders in Poland.
           Validation of disease – specific Autoimmune Bullous Disease Quality of
           Life (ABQOL) and the Treatment Autoimmune Bullous Disease Quality of Life
           (TABQOL) questionnaires
    • Authors: Agnieszka Kalinska-Bienias; Beata Jakubowska; Cezary Kowalewski; Dedee F. Murrell; Katarzyna Wozniak
      Pages: 92 - 96
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Agnieszka Kalinska-Bienias, Beata Jakubowska, Cezary Kowalewski, Dedee F. Murrell, Katarzyna Wozniak
      Purpose Autoimmune bullous dermatoses (AIBD) are rare, severe diseases resulting from some antibodies activity against the different adhesion structures within the skin and/or mucosa. Few studies investigated quality of life (QOL) in AIBD by generic and dermatology-specific instruments, all reporting strong impact on QOL. Recently, disease-specific measurement tools have been developed: Autoimmune Bullous Disease Quality of Life (ABQOL) and Treatment of Autoimmune Bullous Disease Quality of Life (TABQOL) questionnaires. The aim of this study was to test the reliability and validity of ABQOL and TABQOL by developing the first foreign language versions and to evaluate ABQOL and TABQOL in Polish patients. Material and methods The study enrolled 80 patients from the tertiary referral center for AIBD at the outpatient clinic or on admission to the hospital. Sixty six patients completed the 17-item questionnaires of each ABQOL and TABQOL at day 0 and after 5–7 days. Both questionnaires were translated into Polish according to protocol. Results The internal consistency and test–retest reliability were high (Cronbach α =0.95 for ABQOL, α =0.87 for TABQOL), (R =0.98 for ABQOL, R =0.86 for TABQOL). In convergent validity, the correlation of ABQOL and TABQOL was strong (R =0.81), but low with objective disease activity scales. The strongest impact of AIBD on QOL has been observed in flares and in patients with the onset below 70 years of age. The patients with bullous pemphigoid had the highest QOL compared to other AIBD patients. Conclusions The ABQOL and TABQOL are reliable and valid instruments for the assessment of QOL in AIBD.

      PubDate: 2017-02-16T03:36:08Z
      DOI: 10.1016/j.advms.2016.07.002
  • Gastroesophageal reflux is not associated with short-term variability of
           parasympathetic activity in children
    • Authors: Jan Krzysztof Nowak; Agata Łaźniak; Aleksandra Lisowska; Zdzisława Kycler; Waldemar Bobkowski; Jarosław Walkowiak
      Pages: 103 - 109
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Jan Krzysztof Nowak, Agata Łaźniak, Aleksandra Lisowska, Zdzisława Kycler, Waldemar Bobkowski, Jarosław Walkowiak
      Purpose A lower parasympathetic activity was described in patients with the gastroesophageal reflux disease. We aimed to determine whether gastroesophageal reflux (GER) episodes are associated with a short-term parasympathetic tone variability in children. Methods In order to address this question we performed simultaneous 24-h esophageal multichannel intraluminal impedance-pH and electrocardiographic monitoring in 16 children (age range 6–18 years), including 8 with asthma and 2 with gastroesophageal reflux disease. After describing duration, height, and acidity of 483 GER episodes we also measured parasympathetic-related heart rate variability parameters in 4 time periods: preceding, containing, following GER, and in-between GERs (control). High frequency (HF) power and root-mean square differences of successive R–R intervals (r-MSSD) were assessed in 2.5-min and 1-min periods, respectively. Results We did not identify the searched short-term parasympathetic tone changes. Conclusions In conclusion, GER episodes and their characteristics were not associated with short-term variability of parasympathetic activity in children.

      PubDate: 2017-02-23T05:25:13Z
      DOI: 10.1016/j.advms.2016.11.012
  • Single center experience of subclinical rejections and BK nephropathies by
           kidney allografts’ surveillance biopsies
    • Authors: Beata Naumnik; Jolanta Kowalewska; Tomasz Hryszko; Jerzy Glowiński; Magdalena Durlik; Michal C. Myśliwiec
      Pages: 110 - 115
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Beata Naumnik, Jolanta Kowalewska, Tomasz Hryszko, Jerzy Glowiński, Magdalena Durlik, Michal C. Myśliwiec
      Purpose Acute rejection of the kidney allograft remains the most important factor affecting the long-term graft outcome and is a major predictor of development of chronic damage and graft loss. Several studies have shown that early detection and treatment of subclinical rejection episodes may be beneficial for the graft outcome. The role of protocol (surveillance) biopsies and the value of donor specific antibodies (DSA) monitoring are still debatable. Methods This is a prospective observational study involving seventeen kidney recipients transplanted in north-eastern part of Poland who underwent “zero”, 3-month and 12-month allograft biopsies as well as DSA assessment. Results Histologic analysis of the biopsies showed subclinical acute cellular rejection in 17.6% of patients (two tubulointerstitial, one vascular) at 3-months post transplantation, and additional case of borderline rejection at the 12-month point. Moreover, two cases (11.8%) of polyomavirus BK nephropathy were diagnosed (one at 3 and one at 12 month point). None of the patients developed de novo DSA. Conclusions Our protocol biopsies allowed us to detect significant proportion of patients with subclinical, but histologically relevant acute cellular rejection and BK nephropathy. Early therapeutic intervention had beneficial effects in a 4-year follow up.

      PubDate: 2017-03-02T07:56:06Z
      DOI: 10.1016/j.advms.2016.07.005
  • Profiling of selected angiogenesis-related genes in serous ovarian cancer
    • Authors: Paweł Kuć; Radosław Charkiewicz; Dagmara Klasa-Mazurkiewicz; Tomasz Milczek; Barbara Mroczko; Jacek Nikliński; Piotr Laudański
      Pages: 116 - 120
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Paweł Kuć, Radosław Charkiewicz, Dagmara Klasa-Mazurkiewicz, Tomasz Milczek, Barbara Mroczko, Jacek Nikliński, Piotr Laudański
      Purpose Since angiogenesis plays an important role in the pathogenesis of ovarian cancer the aim of the study was to compare the expression of the most relevant angiogenesis-related genes in serous ovarian cancer samples. Genes were divided into 5 subgroups according to their angiogenic potential: growth factors and their receptors; cytokines/chemokines; adhesion molecules and other matrix related proteins; transcriptions factors and signaling molecules; morphogenic factors, and angiogenesis inhibitors. Materials/methods Twenty-nine patients were involved in the study: 20 with serous ovarian cancer and 9 healthy controls. All neoplasms were confirmed by histopathological examination. Healthy ovarian control samples were obtained from women diagnosed with fibroids and had previously scheduled operations. Real-time PCR gene arrays were used to examine the expression of 84 human angiogenesis-related genes and expression of selected proteins was assessed with ELISA. Results Significantly higher expressions of 46 genes were found in the ovarian cancer group compared to the healthy control group. By the use of ELISA we confirmed the expression of three proteins i.e.: angiopoietin-2, angiopoietin-like protein 3, and angiopoietin receptor 2. Only angiopoietin-2 and angiopoietin receptor 2 showed significant differences between ovarian cancer and healthy controls. Conclusions Changes in the expression of selected genes associated with angiogenesis may add new information to the pathogenesis of ovarian cancer. Although the angiopoietin-2 signaling pathway may play an important role in neovascularization in ovarian cancer, the role of angiopoietin-like protein 3 is yet to be established.

      PubDate: 2017-02-23T05:25:13Z
      DOI: 10.1016/j.advms.2016.11.005
  • Classical rather than genetic risk factors account for high cardiovascular
           disease prevalence in Lithuania: A cross-sectional population study
    • Authors: Neringa Burokienė; Ingrida Domarkienė; Laima Ambrozaitytė; Ingrida Uktverytė; Raimonda Meškienė; Dovilė Karčiauskaitė; Vytautas Kasiulevičius; Virginijus Šapoka; Vaidutis Kučinskas; Zita Aušrelė Kučinskienė
      Pages: 121 - 128
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Neringa Burokienė, Ingrida Domarkienė, Laima Ambrozaitytė, Ingrida Uktverytė, Raimonda Meškienė, Dovilė Karčiauskaitė, Vytautas Kasiulevičius, Virginijus Šapoka, Vaidutis Kučinskas, Zita Aušrelė Kučinskienė
      Purpose Cardiovascular disease (CVD) mortality accounts for 54% of all deaths in Lithuania, making it the highest among all of the European Union countries. We evaluated the prevalence of several CVD risk factors, including lifestyle, blood biochemistry and genetic predisposition to determine the reasons behind significantly increased CVD prevalence in Lithuania. Materials and methods In total 435 volunteers of Lithuanian ethnicity and stable geographic settlement for 3 generations, had their anthropometric, biochemical and behavioural risk factors measured. A randomly selected sample of 166 volunteers had their 60 CVD risk alleles genotyped. The prevalence of risk alleles and cumulative CVD genetic risk score were compared with population of North-West European origin (CEU) using data from the phase 3 HapMap project. Results CVD was present in 33.8% of study volunteers, 84% of participants consumed alcohol, 21% were current smokers and only 30% of participants engaged in higher levels of physical activity. Also, the average BMI (males 28.3±4.3kg/m2, females 27.3±5.0kg/m2), total cholesterol (males 6.1±1.2mmol/L, females 6.2±1.0mmol/L) and LDL-cholesterol (males 4.1±1.1mmol/L, females 4.1±1.0mmol/L) were above the normal values. The cumulative genetic susceptibility to develop CVD in Lithuanians was only 1.4% higher than in CEU population. Conclusions High BMI and poor population plasma lipid profile are the major contributing factors to high CVD mortality and morbidity in Lithuania. Smoking, alcohol consumption and preliminary genetic predisposition results do not explain the difference in CVD mortality between the Lithuanian and wider European populations. CVD prevention programmes in Lithuania should primarily focus on weight loss and improving blood lipid control.

      PubDate: 2017-03-02T07:56:06Z
      DOI: 10.1016/j.advms.2016.08.005
  • Orthodontic treatment modifies the oxidant–antioxidant balance in saliva
           of clinically healthy subjects
    • Authors: Piotr Buczko; Małgorzata Knaś; Monika Grycz; Izabela Szarmach; Anna Zalewska
      Pages: 129 - 135
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Piotr Buczko, Małgorzata Knaś, Monika Grycz, Izabela Szarmach, Anna Zalewska
      Purpose The aim of our study was to analyse salivary markers of oxidative stress and an antioxidant response in clinically healthy subjects with fixed orthodontic appliances. Material/methods 37 volunteers were included in the study. Unstimulated (UWS) and stimulated (SWS) whole saliva were analysed for oxidative and antioxidant status and nickel levels immediately before the insertion of the appliances, an one week after and twenty four weeks after the insertion of fixed appliances. Results A significant increase in tiobarbituric acid reactive substance (TBARS) and total oxidant status (TOS) one week, and total protein concentration twenty four weeks after the attachment of orthodontic appliances was found in the saliva. The markers of antioxidant status: superoxide dismutase (SOD), catalase (CAT), uric acid (UA), peroxidase (Px), and total antioxidant status (TAS) were not changed in all periods in UWS. In SWS a significant decrease in SOD1 and CAT was found whereas Px was increased one week after treatment and UA twenty four weeks following treatment. TAS was decreased in UWS and SWS twenty four weeks after orthodontic treatment. Oxidative status index (OSI) was elevated both in UWS and SWS one week after orthodontic treatment in comparison to the results obtained before and twenty four weeks. One week after treatment an increased concentration of nickel was also observed. Conclusions Orthodontic treatment modifies the oxidative–antioxidative balance in the saliva of clinically healthy subjects. Increased nickel concentration in saliva, released from orthodontic appliances, seems to be responsible for changes in the oxidative status of the saliva.

      PubDate: 2017-03-02T07:56:06Z
      DOI: 10.1016/j.advms.2016.11.004
  • Brain morphometric analysis predicts decline of intelligence quotient in
           children with sickle cell disease: A preliminary study
    • Authors: Rong Chen; Jaroslaw Krejza; Michal Arkuszewski; Robert A. Zimmerman; Edward H. Herskovits; Elias R. Melhem
      Pages: 151 - 157
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Rong Chen, Jaroslaw Krejza, Michal Arkuszewski, Robert A. Zimmerman, Edward H. Herskovits, Elias R. Melhem
      Purpose For children with sickle cell disease (SCD) and at low risk category of stroke, we aim to build a predictive model to differentiate those with decline of intelligence-quotient (IQ) from counterparts without decline, based on structural magnetic-resonance (MR) imaging volumetric analysis. Materials and methods This preliminary prospective cohort study included 25 children with SCD, homozygous for hemoglobin S, with no history of stroke and transcranial Doppler mean velocities below 170cm/s at baseline. We administered the Kaufman Brief Intelligence Test (K-BIT) to each child at yearly intervals for 2–4 years. Each child underwent MR examination within 30 days of the baseline K-BIT evaluation date. We calculated K-BIT change rates, and used rate of change in K-BIT to classify children into two groups: a decline group and a non-decline group. We then generated predictive models to predict K-BIT decline/non-decline based on regional gray-matter (GM) volumes computed from structural MR images. Results We identified six structures (the left median cingulate gyrus, the right middle occipital gyrus, the left inferior occipital gyrus, the right fusiform gyrus, the right middle temporal gyrus, the right inferior temporal gyrus) that, when assessed for volume at baseline, are jointly predictive of whether a child would suffer subsequent K-BIT decline. Based on these six regional GM volumes and the baseline K-BIT, we built a prognostic model using the K* algorithm. The accuracy, sensitivity and specificity were 0.84, 0.78 and 0.86, respectively. Conclusions GM volumetric analysis predicts subsequent IQ decline for children with SCD.

      PubDate: 2017-03-08T13:00:26Z
      DOI: 10.1016/j.advms.2016.09.002
  • Analysis of antiproliferative effect of Chamerion angustifolium water
           extract and its fractions on several breast cancer cell lines
    • Authors: Audrius Maruška; Rasa Ugenskienė; Danguolė Raulinaitytė; Elona Juozaitytė; Vilma Kaškonienė; Tomas Drevinskas; Ada Stelmakienė; Ieva Akuneca; Tomas Makaravičius; Nicola Tiso; Violeta Bartkuvienė; Olga Kornyšova; Ona Ragažinskienė; Kristina Ramanauskienė; Vitalis Briedis
      Pages: 158 - 164
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Audrius Maruška, Rasa Ugenskienė, Danguolė Raulinaitytė, Elona Juozaitytė, Vilma Kaškonienė, Tomas Drevinskas, Ada Stelmakienė, Ieva Akuneca, Tomas Makaravičius, Nicola Tiso, Violeta Bartkuvienė, Olga Kornyšova, Ona Ragažinskienė, Kristina Ramanauskienė, Vitalis Briedis
      Purpose To evaluate the antiproliferative effect of the aerial part of Chamerion angustifolium (L.) Holub. (Onagraceae) extract and its fractions in vitro. This is the first study on the anti-proliferative effect of C. angustifolium on 3 distinct breast cancer cell lines. Material/methods Breast cancer cell lines MCF7, MDA-MB-468 and MDA-MB-231 were exposed to different concentrations of the water extract of C. angustifolium, where DPPH radical scavenging activity was 0.018–0.443mg/ml, expressed in rutin equivalents. Cell growth was analyzed after 24, 48 and 72h of incubation. Solid-phase extraction was applied for the fractionation of C. angustifolium water extract and MDA-MB-468 cell line growth was tested using different fractions. Results The concentrations corresponding to radical scavenging activity of 0.117 and 0.266mg/ml caused MCF7 cells growth inhibition, while in the samples exposed to the highest concentration (0.355 and 0.443mg/ml) no proliferation was register, suggesting cell death. MDA-MB-468 cell analysis showed similar responses. MDA-MB-231 demonstrated cell growth inhibition following the exposure to all analyzed high extract doses (0.117–0.443mg/ml). MDA-MB-468 cells were selected to evaluate the effect of fractions. In the samples exposed to the fraction containing the highest amount (91%) of oenothein B, at the concentration of 0.117mg/ml a pronounced cell growth inhibition while at higher concentrations (0.266 and 0.443mg/ml) no cell proliferation was observed. Conclusions The consumption of C. angustifolium herb can be advantageous, alongside with conventional breast cancer treatment.

      PubDate: 2017-03-08T13:00:26Z
      DOI: 10.1016/j.advms.2016.08.002
  • Nicotine can modulate the effects of the mesenchymal stem cells on
    • Authors: Samira Pourtayeb; Seyyed Meysam Abtahi Froushani
      Pages: 165 - 170
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Samira Pourtayeb, Seyyed Meysam Abtahi Froushani
      Purpose It has been revealed that mesenchymal stem cells (MSCs) express some of the nicotinic receptor subunits. Moreover, the crosstalk between MSCs and neutrophils is not far-fetched. Therefore, the aim of the present study is to determine the role of nicotine on the effects of MSCs on neutrophils. Methods After the isolation of mesenchymal stem cells from the bone marrow of rats, these cells were pulsed with different concentrations of nicotine (0, 0.1, 0.5, and 1μM) for different periods (24, 48, and 72h). Then, the neutrophils were co-cultured with MSCs for 4h and the functions of neutrophils were evaluated. Results The obtained findings showed that MSCs pulsed with nicotine significantly enhanced the viability and the phagocytic activity of co-cultured neutrophils and simultaneously, decreased the production of reactive oxygen substances (ROS), induced by f-MLP in neutrophils, more profound than MSCs without treatment. Moreover, MSCs, pulsed with nicotine at low to moderate concentrations, preserved the respiratory burst, triggered by opsonized yeast in neutrophils. Nevertheless, a high concentration of nicotine can interfere with the latter aspect of the crosstalk between MSCs and neutrophils. Conclusion The obtained data can offer a new insight into the potential mechanisms, underlying the immunomodulatory effects of nicotine.

      PubDate: 2017-03-08T13:00:26Z
      DOI: 10.1016/j.advms.2016.07.006
  • The fecal occult blood test as a tool for improved outpatient
           qualification for colonoscopy. A single-center experience and 10-year
           follow-up survey
    • Authors: Zbigniew Banaszkiewicz; Jacek Budzyński; Krzysztof Tojek; Paweł Jarmocik; Jacek Frasz; Marcin Mrozowski; Maciej Świtoński; Arkadiusz Jawień
      Pages: 171 - 176
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Zbigniew Banaszkiewicz, Jacek Budzyński, Krzysztof Tojek, Paweł Jarmocik, Jacek Frasz, Marcin Mrozowski, Maciej Świtoński, Arkadiusz Jawień
      Purpose Colonoscopy is not widely and easily available in all countries, even for symptomatic patients. This is one of the causes of tumors not being diagnosed until an advanced stage. The aim of this study was to estimate the efficacy of the fecal occult blood test (FOBT) in the diagnostic work-up of outpatients referred to a colorectal unit due to indistinct abdominal symptoms. Patients/methods Among 10418 consecutive symptomatic individuals referred to the outpatient clinic, an immunochemical FOBT (Hem-Check 1®) was recommended for 9432 patients with indistinct symptoms as a tool for qualifying them for colonoscopy. All the subjects were treated according to their diagnosis and followed-up for the next 10 years. Results Colorectal cancer (CRC) was diagnosed in 535 individuals: 393/986 (39.9%) among patients with red-flag symptoms, and 142/951 (14.9%) of individuals with indistinct symptoms and a positive FOBT. In the latter group, less-advanced tumors, classed as such using Dukes’ classification, were twice as common and more advanced CRC occurred twice as seldom than in the former. Cancer recurrence-free and overall survival periods after surgical treatment for CRC were significantly longer in patients with indistinct symptoms who qualified for diagnostic procedures on the basis of a positive FOBT. Conclusions Patients with symptoms suggesting organic colon disease had a worse prognosis compared to individuals with non-specific symptoms. If bowel endoscopy is not widely and easily available, qualification for colonoscopy on the basis of alarm symptoms and a positive FOBT seems to be an effective strategy in early CRC diagnosis.

      PubDate: 2017-03-08T13:00:26Z
      DOI: 10.1016/j.advms.2016.08.003
  • Variations of renal tissue oxygenation during abdominal compartment
           syndrome and sepsis
    • Authors: Stavros Kalfadis; Vaia Nalbanti; Orestis Ioannidis; George Porfiriou; Dimitrios Botsios; Konstantinos Tsalis
      Pages: 177 - 185
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Stavros Kalfadis, Vaia Nalbanti, Orestis Ioannidis, George Porfiriou, Dimitrios Botsios, Konstantinos Tsalis
      Purpose This experimental study was designed to evaluate the renal tissue oxygenation under the coexistence of abdominal compartment syndrome and sepsis. Material and methods Fourteen non-breed dogs were divided into two groups: the control group (8) and the study group (6). Sepsis was established with intravenous endotoxin infusion at 100μg/kg for over 30min. Insufflation of CO2 in the peritoneal cavity was used for the increase in intra-abdominal pressure (IAP). A special catheter placed and fixed in the renal cortex at a depth of 3mm from the renal capsule was used for the measurement of renal tissue oxygenation. Results Study parameters were recorded at the starting phase, at IAP of 15mmHg and 30mmHg and after decompression of the abdomen in the control group, and at the same intervals plus the induction of sepsis, prior to increasing abdominal pressure, in the study group. With the elevation of the IAP a reduction of renal tissue oxygenation presents itself, which is more pronounced in the presence of sepsis, especially for IAP over 15mmHg. Like other parameters, after abdominal decompression the renal tissue oxygenation returns to the initial levels, independently of sepsis. Conclusions The afferent arterioles vasoconstriction, which takes place during sepsis, and the intra-renal shunt, which occurs and leads to blood diversion to the medulla from the renal cortex due to the combination of intra-abdominal hypertension (IAH) and sepsis, seem to explain this finding.

      PubDate: 2017-03-08T13:00:26Z
      DOI: 10.1016/j.advms.2016.08.004
  • Protection against cerebral infarction by Withaferin A involves inhibition
           of neuronal apoptosis, activation of PI3K/Akt signaling pathway, and
           reduced intimal hyperplasia via inhibition of VSMC migration and matrix
    • Authors: Qi-Zhi Zhang; Yu-Dong Guo; Hao-Mei Li; Rui-Zheng Wang; Shou-Gang Guo; Yi-Feng Du
      Pages: 186 - 192
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Qi-Zhi Zhang, Yu-Dong Guo, Hao-Mei Li, Rui-Zheng Wang, Shou-Gang Guo, Yi-Feng Du
      Purpose Stroke is a major public health concern with high rates of morbidity and mortality worldwide. Cerebral ischemia and infarction are commonly associated with stroke. Currently used medications, though effective, are also associated with adverse effects. Development of effective neuroprotective agents with fewer side effects would be of clinical value. We evaluated the effects of Withaferin A (WA), a steroidal lactone derived from the plant Withania somnifera, on experimentally induced cerebral infarction. Materials and methods The ability of WA to inhibit neuroapoptosis and modulate vascular smooth muscle cell (VSMC) migration and PI3K/Akt signaling was assessed. Separate groups of Sprague Dawley rats were subjected to cerebral occlusion and reperfused for 24h. Results WA treatment (25, 50 or 100mg/kg bodyweight) significantly reduced the infarct area in a carotid ligation model; WA reduced intimal hyperplasia and proliferating cell nuclear antigen (PCNA)-positive cell counts. Western blotting analysis revealed significantly suppressed PI3K/Akt signaling following cerebral ischemia/reperfusion injury. WA supplementation was found to downregulate apoptotic pathway proteins. WA suppressed PTEN and enhanced p-Akt and GSK-3β levels and elevated mTORc1, cyclinD1 and NF-κB p65 expression, suggesting activation of the PI3K/Akt pathway. In vitro studies with PDGF-stimulated A7r5 cells revealed that WA exposure severely downregulated matrix metalloproteinases (MMP)-2 and -9 and inhibited migration of A7r5 cells. Additionally, WA reduced the proliferation of A7r5 cells significantly. Conclusions WA exerted neuroprotective effects by activating the PI3K/Akt pathway, modulating the expression of MMPs, and inhibiting the migration of VSMCs.

      PubDate: 2017-03-08T13:00:26Z
      DOI: 10.1016/j.advms.2016.09.003
  • Beta2-adrenergic receptor gene haplotypes and bronchodilator response in
           Egyptian patients with chronic obstructive pulmonary disease
    • Authors: Mohammad H. Hussein; Khaled E. Sobhy; Irene M. Sabry; Ahmed T. El Serafi; Eman A. Toraih
      Pages: 193 - 201
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Mohammad H. Hussein, Khaled E. Sobhy, Irene M. Sabry, Ahmed T. El Serafi, Eman A. Toraih
      Purpose Chronic obstructive pulmonary disease (COPD) is a multi-factorial disorder caused by environmental determinants and genetic risk factors. Understanding the genetic predisposition of COPD is essential to develop personalized treatment regimens. Beta2-adrenergic receptor (ADRB2) gene polymorphisms have been implicated in the pathogenesis of obstructive pulmonary diseases. This study was conducted to assess the genetic association between Arg16Gly and Gln27Glu polymorphisms and COPD in the Egyptian patients, and to analyze their impact on the clinical outcome and therapeutic response. Patients/methods The study population included 115 participants (61 COPD patients and 54 healthy controls) were genotyped for the Arg16Gly (rs1042713) and Gln27Glu (rs1042714) polymorphisms. Pulmonary function test was done and repeated in patients after salbutamol inhalation. Results The Gly16 and Gln27 alleles represented 57% and 70% of the whole study population, and only 3 haplotypes were detected; Arg16/Gln27, Gly16/Gln27, and Gly16/Glu27. Genotypes and haplotypes homozygous for Arg16 and Gln27 were more likely to develop COPD (p <0.05). However, individuals carrying Glu27 allele conferred protection against COPD development (p =0.002). Furthermore, Arg16 genotypes and haplotypes were significantly associated with higher grades of dyspnea, more COPD symptoms and frequent exacerbations. In contrast, patients carrying Glu27 allele had better bronchial airway responsiveness to β2-agonists. Conclusions Our findings suggested that the ADRB2 gene polymorphisms may have vital role in COPD risk, severity, and bronchodilator response among Egyptian population. Larger epidemiological studies are needed for results validation.

      PubDate: 2017-03-20T18:58:46Z
      DOI: 10.1016/j.advms.2016.07.008
  • How much information about embryo implantation potential is included in
           morphokinetic data? A prediction model based on artificial neural networks
           and principal component analysis
    • Authors: Robert Milewski; Agnieszka Kuczyńska; Bożena Stankiewicz; Waldemar Kuczyński
      Pages: 202 - 206
      Abstract: Publication date: March 2017
      Source:Advances in Medical Sciences, Volume 62, Issue 1
      Author(s): Robert Milewski, Agnieszka Kuczyńska, Bożena Stankiewicz, Waldemar Kuczyński
      Purpose The aim of this study was to answer the question of how much information about embryo implantation potential can be obtained from morphokinetic parameters through the creation a predictive model based on morphokinetic information and using advanced data-mining and artificial intelligence methods. Materials and methods Time-lapse recordings of 610 embryos were included in the analysis. For each embryo, absolute (t 2, t 3, t 4, t 5) and relative (cc2 and s2) morphokinetic parameters were collected. Additionally, fragmentation levels assessed at t 2, t 3, t 4 and t 5 time-points, presence of multinucleation, evenness of blastomeres after the first and second division and women's age were included in the analysis. Results The created predictive model of implantation was constructed on the basis of two advanced data-mining methods: principal component analysis (PCA) and artificial neural networks (ANN). The receiver operating characteristic (ROC) curve constructed for the created model demonstrated its good quality. The area under the ROC curve was AUC=0.75 with a 95% confidence interval (0.70, 0.80). Finally, the model was verified on a validation set and the results were also good, although slightly weaker: AUC=0.71, with a 95% confidence interval (0.59, 0.84). Conclusions The combination of two data-mining algorithms: PCA and ANN may be considered a method which can extract virtually all the available information from data. This methodology is indeed efficient, but models presented in the literature are also effective and close to the limit of the maximal information which can be extracted from morphokinetic data. The limit can be determined at the level of AUC value marginally above 0.7.

      PubDate: 2017-04-10T21:26:22Z
      DOI: 10.1016/j.advms.2017.02.001
  • CS-PEG decorated PLGA nano-prototype for delivery of bioactive compounds:
           A novel approach for induction of apoptosis in HepG2 cell line
    • Authors: Ahmed A. Abd-Rabou; Hanaa H. Ahmed
      Abstract: Publication date: Available online 12 March 2017
      Source:Advances in Medical Sciences
      Author(s): Ahmed A. Abd-Rabou, Hanaa H. Ahmed
      Purpose Polymer-based nanoparticles are used as vectors for cancer drug delivery. The bioactive compounds (quercetin, ellagic acid and gallic acid) are well known to be not only antioxidants but also chemopreventive candidates against various types of cancers. To circumvent the low bioavailability and the short half-life time obstacles, we hypothesized a novel PLGA nano-platform functionalized with CS and PEG to encapsulate these phytochemicals. This encapsulation will protect the compounds from the phagocytic uptake and deliver PLGA-CS-PEG nano-prototype with high biodegradability and biosafety. Materials and methods Three consequent types of PLGA-based nanocomposites were prepared and characterized. Furthermore, we investigated the newly synthesized nano-formulations against human hepatocellular carcinoma (HepG2) and colorectal cancer (HCT 116) cell lines using cell growth inhibition assays, followed by apoptosis and necrosis assays using flow cytometry to detect the underlying mechanism of HepG2 cell death. Results Through Malvern Zeta Sizer, we recorded that the average diameters of the nano-prototypes ranged from 150 to 300nm. Regarding the cytotoxic activity of quercetin, ellagic acid, and gallic acid-encapsulated PLGA, PLGA-CS, and PLGA-CS-PEG nano-prototypes has been found that they reduce the IC50s of the HepG2 cells values by 2.2, 2.9, 2.8-folds, 1, 1.5, 2.7-folds, and 0.9, 0.7, 1.5-folds, respectively. Mechanistically, the nano-platforms of quercetin seem to be dependent on both apoptosis and necrosis, while those of ellagic acid and gallic acid are mainly dependent on apoptosis. Conclusions CS-PEG-blended PLGA nano-delivery system of quercetin, ellagic acid and gallic acid can potentiate apoptosis-mediated cell death in HepG2 cell line.
      Graphical abstract image

      PubDate: 2017-03-16T17:39:48Z
      DOI: 10.1016/j.advms.2017.01.003
  • Anti-parietal cell antibodies-diagnostic significance
    • Authors: Ewa Rusak; Agata Chobot; Agnieszka Krzywicka; Janet Wenzlau
      Pages: 175 - 179
      Abstract: Publication date: Available online 13 January 2016
      Source:Advances in Medical Sciences
      Author(s): Ewa Rusak, Agata Chobot, Agnieszka Krzywicka, Janet Wenzlau
      Anti-parietal cell antibodies (APCA) are an advantageous tool for screening for autoimmune atrophic gastritis (AAG) and pernicious anemia (PA). The target for APCA is the H+/K+ ATP-ase. It has been demonstrated, that APCA target both, the alpha, and beta subunits of the proton pump, although the major antigen is the alpha subunit. Circulating serum APCA can be detected by means of immunofluorescence, enzyme-linked immunosorbent assay – currently the most commonly used method, and radioimmunoprecipitation assay (RIA) – the 4A subunit has been optimized as a molecular-specific antigen probe. RIA is the most accurate method of antibody assessment, characterized by highest sensitivity. APCA can be found in 85–90% of patients with PA. Their presence is not sufficient for diagnosis, because they are not specific for PA as they are also found in the circulation of individuals with other diseases. APCA are more prevalent in the serum of patients with T1D, autoimmune thyroid diseases, vitiligo, celiac disease. People with autoimmune diseases should be closely screened for AAG/PA. The anemia develops longitudinally over many years in APCA-positive patients, symptomless, slowly promotes atrophy of the gastric mucosa and parietal cells. APCA are present in 7.8–19.5% of the general healthy adult population. A fraction of these sero-positive people, will never develop AAG or PA. An interesting and not fully explained question is whether APCA presence is related to Helicobacter pylori infection. APCA are found in up to 20.7% of these patients. H. pylori is implicated as one of the candidates causing AAG.

      PubDate: 2016-01-14T16:25:26Z
      DOI: 10.1016/j.advms.2015.12.004
  • Occlusion trajectory and a concept of a device for testing operating life
           of dentures
    • Authors: Marek Jałbrzykowski; Marcin Derlatka; Dariusz Urban
      Pages: 180 - 186
      Abstract: Publication date: Available online 14 January 2016
      Source:Advances in Medical Sciences
      Author(s): Jałbrzykowski Marek, Derlatka Marcin, Urban Dariusz
      Purpose This paper presents an original method for the assessment of occlusion trajectory. On this basis, a special device for the assessment of operating life of dentures was designed. Material/methods For this purpose, the SMART system by BTS for a comprehensive movement analysis was used. In order to analyze occlusion trajectory, characteristic points on patients’ heads were appointed in which markers were placed, in accordance with the rules of measurement in dentistry. Markers’ movement was recorded by means of 6 cameras, and then composition of coordinates was performed in a 3D system. Results In this way, curves representing movements of the characteristic points were plotted which, after the composition, with a considerable approximation, can be regarded as occlusion trajectory. Conclusions On the basis of the obtained results, a thesis was put forward to the effect that traditional tribological testing machines based on systems of the pin-on-disc, ball-on-disc, etc. types, due to the simplicity of their working movements, are not adequately precise for the purpose of operational assessment of elements of prosthodontics. On this basis, a tribological node for a specialist testing machine for the assessment of operating life of dentures was designed.

      PubDate: 2016-01-14T16:25:26Z
      DOI: 10.1016/j.advms.2015.12.005
  • Expression of IL-1 and IL-6 and their natural regulators in leukocytes of
           B-cell chronic lymphocytic leukaemia patients
    • Authors: Marzena Garley; Ewa Jabłońska; Jolanta Sawicka-Powierza; Janusz Kłoczko; Jarosław Piszcz; Magdalena Kakareko; Wioletta Ratajczak-Wrona; Angelika Charkiewicz; Wioleta Omeljaniuk; Andrzej Szpak
      Pages: 187 - 192
      Abstract: Publication date: Available online 13 January 2016
      Source:Advances in Medical Sciences
      Author(s): Marzena Garley, Ewa Jabłońska, Jolanta Sawicka-Powierza, Janusz Kłoczko, Jarosław Piszcz, Magdalena Kakareko, Wioletta Ratajczak-Wrona, Angelika Charkiewicz, Wioleta Omeljaniuk, Andrzej Szpak
      Purpose The purpose of the study was the assessment of the expression of IL-1β and IL-6, and the proteins regulating their biological activity, namely IL-1RII, IL-1Ra, as well as sIL-6Rα, sgp-130 in leukemic lymphocytes and autologous neutrophils of B-CLL patients. Methods/results Western blot analysis showed a decreased expression of IL-1β and IL-6 in the leukocytes of B-CLL patients. Decreased expression of sIL-6Rα has been observed in lymphocytes, with a simultaneous increase of expression in PMNs. Lower expression of sgp-130 was found in B cells while its expression was elevated in the neutrophils of patients in early stages of the disease. Conclusions The changes observed in the expression of IL-1 and IL-6 seem to exclude their immediate involvement in the progress of B-CLL. However, the presented changes in the expression of proteins regulating IL-1β and IL-6 in PMNs indicate a potential role of early immune response cells also in advanced stages of the disease.

      PubDate: 2016-01-14T16:25:26Z
      DOI: 10.1016/j.advms.2015.12.006
  • Changes of glycosylation of IgG in rheumatoid arthritis patients treated
           with methotrexate
    • Authors: Ewa Gińdzieńska-Sieśkiewicz; Iwona Radziejewska; Izabela Domysławska; Piotr Adrian Klimiuk; Agnieszka Sulik; Joanna Rojewska; Halina Gabryel-Porowska; Stanisław Sierakowski
      Pages: 193 - 197
      Abstract: Publication date: Available online 14 January 2016
      Source:Advances in Medical Sciences
      Author(s): Ewa Gińdzieńska-Sieśkiewicz, Iwona Radziejewska, Izabela Domysławska, Piotr Adrian Klimiuk, Agnieszka Sulik, Joanna Rojewska, Halina Gabryel-Porowska, Stanisław Sierakowski
      Purpose In patients with active rheumatoid arthritis (RA) decrease of galactosylation is correlated with disease activity. The aim of our study was to evaluate an effect of methotrexate therapy on glycosylation disturbances of IgG in RA patients. Materials/methods IgG glycosylation in 40 patients with active RA treated with methotrexate for 12 months prior to and after treatment were compared. The control group consisted of 20 healthy volunteers. IgG glycosylation was assessed using biotinylated lectins and immunosorbent ELISA assay. For galactose specificity Datura stramonium lectin (DSA), for sialic acid Sambucus nigra (SNA) and Maackia amurensis (MAA) and for fucose residue Areulia auranta (AAA) lectins were used. Results In RA-cases N-glycan galactosylation and sialylation of IgG before treatment were significantly lower than in healthy subjects (for DSA, MAA lectins p <0.001 and SNA p <0.05). Significant increase of IgG galactosylation and sialylation in RA patients after therapy (for DSA, MAA and SNA lectin p <0.05) was detected. Moreover the glycosylation disturbances of N-glycan IgG were strongly associated with changes of disease activity based on disease activity score. For fucose residues significantly higher absorbency of AAA lectin in RA patients before treatment was observed compared to control subjects (p <0.05) and slightly, not significantly decreased after MTX therapy. Conclusions Defect of galactosylation of IgG in RA patients is a useful marker of disease activity that may be used for the assessment of therapy effectiveness. The role of IgG fucosylation and sialylation in RA pathogenesis has still to be determined.

      PubDate: 2016-01-14T16:25:26Z
      DOI: 10.1016/j.advms.2015.12.009
  • Polymorphisms in the type I deiodinase gene and frontal function in
           recurrent depressive disorder
    • Authors: Elżbieta Gałecka; Monika Talarowska; Agata Orzechowska; Paweł Górski; Janusz Szemraj
      Pages: 198 - 202
      Abstract: Publication date: Available online 13 January 2016
      Source:Advances in Medical Sciences
      Author(s): Elżbieta Gałecka, Monika Talarowska, Agata Orzechowska, Paweł Górski, Janusz Szemraj
      Purpose Significant impairment of some psychological functions, including cognitive functioning, has been characteristically found in depressed patients. Memory disturbances may be related to the levels of thyroid hormones (TH) that are under the influence of different mechanisms and molecules, including deiodinase type 1(D1) – an important determinant of circulating triiodothyronine (T3). We investigated the relationship between two functionally known polymorphisms within the DIO1 gene, i.e. DIO1a-C/T and DIO1b-A/G, and cognitive functioning in patients diagnosed with recurrent depressive disorder (rDD). In the planned analysis we mainly concentrated on the frontal function: working memory, executive functions and verbal fluency. Materials and methods Genetic variants were genotyped in 128 patients using a method based on polymerase chain reaction (PCR). Cognitive functions were assessed by the Trail Making Test, the Stroop Test and the Verbal Fluency Test (VFT). Results No significant associations were found between DIO1 polymorphisms and cognitive functioning in rDD. Only the CT and TT genotypes of the DIO1a variant were significantly related to verbal fluency. There were no significant differences between the distribution of the genotypes and demographic/medical variables. Conclusions Based on the study, the examined polymorphisms are not an important risk or protective factor for cognitive impairment in depressive patients. Functional variants within the DIO1 gene that affect triiodothyronine (T3) levels seem not to be associated with cognitive functions. Nevertheless, considering the fact that the DIO1 gene is related to the course and management of depression, further studies on a larger sample size might be suggested.

      PubDate: 2016-01-14T16:25:26Z
      DOI: 10.1016/j.advms.2015.12.008
  • Circumferential strain of carotid arteries does not differ between
           patients with advanced coronary artery disease and group without coronary
    • Authors: Karina Wierzbowska-Drabik; Kamila Cygulska; Urszula Cieślik-Guerra; Barbara Uznańska-Loch; Tomasz Rechciński; Ewa Trzos; Małgorzata Kurpesa; Jarosław D. Kasprzak
      Pages: 203 - 206
      Abstract: Publication date: Available online 14 January 2016
      Source:Advances in Medical Sciences
      Author(s): Karina Wierzbowska-Drabik, Kamila Cygulska, Urszula Cieślik-Guerra, Barbara Uznańska-Loch, Tomasz Rechciński, Ewa Trzos, Małgorzata Kurpesa, Jarosław D. Kasprzak
      Background Speckle tracking echocardiography is widely used for the analysis of myocardial function. Recently, circumferential strain (CS) of carotid arteries was postulated as novel indicator of vascular function. Methods We compared CS of both common carotid arteries (CCA) in the 25 patients with three-vessel coronary artery disease (3VD) (mean age 69±9 years, 9 male) and in 16 age-matched subjects without significant coronary lesions (C) (69±8 years, 7 male). Additionally in 11 patients we estimated pulse wave velocity (PWV) and assessed the correlation between PWV and CS. Short-axis images of arteries were acquired for strain analysis with linear probe of echocardiograph. The assessment of CS was performed off-line by two observers. Results The intraobserver variability for the CS (coefficient of variation) were 4.9 and 5.4% for left and right CCA and interobserver variability were 11.7% and 12.5%, respectively. The mean CS for left and right CCA did not differ between compared groups. We did not find correlation between CS strain and PWV. The only difference was related to the more prevalent plaque presence and thicker intima-media complex (IMT) in 3VD (p =0.0039 for IMT of left CCA and p =0.016 for IMT of right CCA). Conclusion The global CS of CCA, contrary to IMT, did not allow for differentiation between 3VD and C subjects. Despite good feasibility and concordance of CS measurements its clinical significance remains to be established.

      PubDate: 2016-01-14T16:25:26Z
      DOI: 10.1016/j.advms.2015.12.010
  • Dithranol treatment of plaque-type psoriasis increases serum TNF-like weak
           inducer of apoptosis (TWEAK)
    • Authors: Hanna Myśliwiec; Piotr Myśliwiec; Anna Baran; Iwona Flisiak
      Pages: 207 - 211
      Abstract: Publication date: Available online 21 January 2016
      Source:Advances in Medical Sciences
      Author(s): Hanna Myśliwiec, Piotr Myśliwiec, Anna Baran, Iwona Flisiak
      Purpose TNF-like weak inducer of apoptosis (TWEAK) mediates not only apoptosis, but also inflammation, cell growth and angiogenesis. The role of TWEAK in psoriasis remains unknown. The aim of the study was to assess serum levels of TWEAK in psoriatic patients before and after topical treatment with dithranol in relation to the clinical activity of the disease. Material and methods Serum samples were collected from 40 patients with plaque type psoriasis before and after topical treatment with dithranol. The concentrations of serum TWEAK were measured by ELISA and next compared with 16 healthy controls. The data were analyzed with respect to Psoriasis Area and Severity Index (PASI). Results Baseline serum TWEAK concentrations of psoriatic patients (685±166pg/ml) were significantly greater compared to healthy controls (565±110pg/ml). Topical treatment resulted in further increase in serum TWEAK (749±179pg/ml; p <0.01). In case of patients with initial serum TWEAK concentrations above the median, PASI after topical treatment was lower compared to the individuals with initial TWEAK below the median. Conclusion According to the study, serum Tweak was increased in psoriasis patients compared with controls. Moreover, dithranol topical treatment caused further increase in serum TWEAK. Also, a higher effectiveness of topical treatment was observed in case of patients with higher initial TWEAK concentrations. The results suggest a potential role of TWEAK in psoriasis therapy.

      PubDate: 2016-01-23T18:11:49Z
      DOI: 10.1016/j.advms.2016.01.001
  • PET/CT and MRI directed extended salvage radiotherapy in recurrent
           prostate cancer with lymph node metastases
    • Authors: Hans Christian Rischke; Ann-Kristin Eiberger; Natalja Volegova-Neher; Karl Henne; Tobias Krauss; Anca-L. Grosu; Cordula A. Jilg
      Pages: 212 - 218
      Abstract: Publication date: Available online 3 February 2016
      Source:Advances in Medical Sciences
      Author(s): Hans Christian Rischke, Ann-Kristin Eiberger, Natalja Volegova-Neher, Karl Henne, Tobias Krauss, Anca-L. Grosu, Cordula A. Jilg
      Purpose PET/CT directed extended salvage radiotherapy (esRT) of involved lymph-node (LN) regions may be a salvage strategy for patients with nodal recurrent prostate cancer (PCa) after primary therapy or after previous prostate fossa salvage RT. The aim of the study was to determine the time until prostate-specific antigen (PSA) progression, pattern of failure and toxicity after esRT. Material and methods 25 patients with nodal or nodal+local recurrent PCa confirmed by Choline-PET/CT and Magnetic Resonance Imaging (MRI) were treated with esRT at the sites of recurrence. Acute and late toxicity was recorded. In case of subsequent PSA progression, imaging was performed to confirm next relapse. Mean follow-up was 2.9 years. Results According to Choline-PET/CT and MRI findings, 84% (21/25) of esRT were treatment of pelvic only, 12% (3/25) of retroperitoneal only and 4% (1/25) of both pelvic and retroperitoneal regions. 40% (10/25) received concomitant irradiation of the prostatic fossa (after primary radical prostatectomy). Median time to PSA progression of the whole cohort was 19.6 months. Median time to PSA progression for patients with 1–2 PET-positive LN (n =15) was 34.9 months versus median 12.7 months for patients with PET-positive LN≥3 (n =10), p-value: 0.0476. Acute and late toxicity was mild to moderate, no grade-3 adverse events were observed. Conclusion PET/CT and MRI directed esRT of nodal recurrent PCa with or without local recurrence is feasible with low acute and late toxicity. Patients with only one or two PET-positive LN treated by esRT achieved prolonged complete biochemical remission.

      PubDate: 2016-02-08T14:16:15Z
      DOI: 10.1016/j.advms.2016.01.003
  • Association between serum 25-hydroxyvitamin D concentrations and
           prevalence of metabolic syndrome
    • Authors: Shokoufeh Bonakdaran; Farzaneh Fakhraee; Maryam Saberi Karimian; Seyed Reza Mirhafez; Haleh Rokni; Mohsen Mohebati; Mohsen Mazidi; Mina Mousavi; Gordon A. Ferns; Majid Ghayour-Mobarhan
      Pages: 219 - 223
      Abstract: Publication date: Available online 2 February 2016
      Source:Advances in Medical Sciences
      Author(s): Shokoufeh Bonakdaran, Farzaneh Fakhraee, Maryam Saberi Karimian, Seyed Reza Mirhafez, Haleh Rokni, Mohsen Mohebati, Mohsen Mazidi, Mina Mousavi, Gordon A. Ferns, Majid Ghayour-Mobarhan
      Purpose There is now good evidence that 25-hydroxyvitamin D (25OHD) status may have an important impact on the development and progression of cardiovascular disease. Because of the potential involvement of vitamin D deficiency in blood pressure control and immune responses, we aimed to investigate whether there was a relationship between 25OHD status and the prevalence of metabolic syndrome in an Iranian population. Material/methods The study was carried out on a sample of 846 subjects [357(42.19%) males and 489(57.80%) females], derived from MASHAD STUDY. Serum 25OHD levels were measured using a competitive electroluminescence protein binding assay. Anthropometric indices were measured using standard protocols. Results Serum 25OHD was 12.7 (6.8–18.4) ng/ml in the metabolic syndrome (MetS) group and 14.1 (8.8–19.0) ng/ml in the group without metabolic syndrome (P =0.43). The frequency of vitamin D deficiency was 80.7% and 79.0% in subjects with or without metabolic syndrome in Iranian population. Conclusions We found no significant difference in serum 25OHD concentrations between individuals with or without MetS and no significant linear relationship between serum 25OHD and several CVD risk factors.

      PubDate: 2016-02-08T14:16:15Z
      DOI: 10.1016/j.advms.2016.01.002
  • Vitamin D and functional arterial parameters in postmenopausal women with
           metabolic syndrome
    • Authors: Jolanta Dadonienė; Alma Čypienė; Egidija Rinkūnienė; Jolita Badarienė; Jelizaveta Burca; Ieva Sakaitė; Goda Kalinauskaitė; Vaiva Kumpauskaitė; Aleksandras Laucevičius
      Pages: 224 - 230
      Abstract: Publication date: Available online 13 January 2016
      Source:Advances in Medical Sciences
      Author(s): Jolanta Dadonienė, Alma Čypienė, Egidija Rinkūnienė, Jolita Badarienė, Jelizaveta Burca, Ieva Sakaitė, Goda Kalinauskaitė, Vaiva Kumpauskaitė, Aleksandras Laucevičius
      Purpose Our cross sectional study aimed to identify the relation between vitamin D level and functional arterial parameters in postmenopausal women with metabolic syndrome. Material and methods 100 postmenopausal women at age 50–65 with diagnosed metabolic syndrome were included in this study. Laboratory tests were performed to determine lipid profile, serum glucose, creatinine, C-reactive protein, serum levels of 25(OH) D, ionized calcium and urine albumin/creatinine ratio. Also non-invasive assessment of arterial function (arterial stiffness, flow-mediated dilatation and carotid artery ultrasound examinations) was performed. Results The mean vitamin D blood concentration was 47.4±16.9nmol/l. The prevalence of modest insufficiency and deficiency of vitamin D was 62%. Vitamin D concentration in samples assembled from January to March was significantly lower than concentration levels from September to November. No significant relationship was observed between vitamin D and endothelial function, arterial stiffness, carotid intima-media thickness. Week negative correlation was stated between mean arterial pressure and 25(OH) D concentration (p =0.04). A positive correlation was found between high density lipoprotein cholesterol and vitamin 25(OH) D (r =0.3, p <0.05). No significant difference between 25(OH) D and other lipoproteins, calcium ions, glucose, albumin/creatinine ratio and C-reactive protein blood concentrations were found. Conclusions The prevalence of vitamin D deficiency in postmenopausal women with metabolic syndrome is high. No relation was found between vitamin D levels and parameters that indicate atherosclerotic vascular lesions. Nevertheless our study revealed the relation between concentrations of vitamin D and mean blood pressure and high density lipoprotein cholesterol.

      PubDate: 2016-01-14T16:25:26Z
      DOI: 10.1016/j.advms.2015.12.007
  • CagA-positive Helicobacter pylori strain containing three EPIYA C
           phosphorylation sites produces increase of G cell and decrease of D cell
           in experimentally infected gerbils (Meriones unguiculatus)
    • Authors: Moacir Ferreira Júnior; Sérgio de Assis Batista; Rafael Calvão Barbuto; Adriana Dias Gomes; Dulciene Maria Magalhães Queiroz; Ivana Duval Araújo; Marcelo Vidigal Caliari
      Pages: 231 - 236
      Abstract: Publication date: Available online 23 February 2016
      Source:Advances in Medical Sciences
      Author(s): Moacir Ferreira Júnior, Sérgio de Assis Batista, Rafael Calvão Barbuto, Adriana Dias Gomes, Dulciene Maria Magalhães Queiroz, Ivana Duval Araújo, Marcelo Vidigal Caliari
      Purpose Human infection by Helicobacter pylori is associated with an increase in the number of gastrin-producing G cells and a concomitant decrease of somatostatin-producing D cells. However, to our knowledge, changes in G and D cell numbers in response to infection with H. pylori CagA-positive strains containing different number of EPIYA-C phosphorylation sites have not been analyzed to date. Therefore, the aim of this study was to perform a quantitative analysis of the number of G and D cells in Mongolian gerbils challenged with H. pylori strains with different numbers of EPIYA-C motifs. Materials and methods Mongolian gerbils were inoculated with isogenic H. pylori strains containing one to three phosphorylation sites. Mucosal fragments were evaluated by morphometry and immunohistochemistry using primary polyclonal rabbit anti-gastrin and anti-somatostatin antibodies. Positive cells were counted using an image analyzer. Results Forty-five days after infection, there was a decrease in the number of D cells and an increase in the G/D cell ratio in the group with three EPIYA-C. Six months after infection, there was a progressive and significant increase in the number of G cells and in the G/D cell ratio, with a concomitant decrease in the number of D cells, especially in the three EPIYA-C group. Conclusions CagA-positive H. pylori strains containing a large number of EPIYA-C phosphorylation sites induce a decrease in D cell number and an increase in G cell number and G/D ratio, which were correlated with the number of inflammatory cells of the lamina propria.

      PubDate: 2016-02-27T10:56:56Z
      DOI: 10.1016/j.advms.2016.02.003
  • Survivin, caspase-3 and MIB-1 expression in astrocytic tumors of various
    • Authors: Agnieszka Lebelt; Robert Rutkowski; Waldemar Och; Kamil Jaczun; Dorota Dziemiańczyk-Pakieła; Robert Milewski; Zenon Mariak; Joanna Reszeć
      Pages: 237 - 243
      Abstract: Publication date: Available online 12 February 2016
      Source:Advances in Medical Sciences
      Author(s): Agnieszka Lebelt, Robert Rutkowski, Waldemar Och, Kamil Jaczun, Dorota Dziemiańczyk-Pakieła, Robert Milewski, Zenon Mariak, Joanna Reszeć
      Purpose Gliomas are the most common primary brain tumors. The etiology is still unclear and the progression from low to high-grade gliomas is frequent. The molecular mechanisms are quite established, however the heterogeneity of glioblastomas force the scientist to look for the new therapeutic targets. The aim of the study was to evaluate the caspase-3 and survivin expression in correlation with MIB-1 expression in gliomas of various grade to assess the apoptosis in gliomas and to determinate new possible targets for the future therapy. Material and methods We identified 131 patients with a histopathological diagnosis of astrocytic tumors (diffuse astrocytoma, anaplastic astrocytoma and glioblastoma). The evaluation of caspase-3, survivin and MIB-1 expression was done using immunohistochemical methods. Results Caspase-3 and survivin expression was observed both in low- and high-grade astrocytomas. The differences in expression were the most evident in glioblastoma group. All primary glioblastomas (31 cases) expressed caspase-3. In secondary glioblastoma group only 17 out of 30 specimens were positive for caspase-3. Survivin expression was observed in 80.6% primary glioblastomas and in all examined secondary glioblastomas and the staining was strong and diffuse in all cases. MIB-1 expression was low in diffuse astrocytomas (DA) and ranged between 1 and 5%. In anaplastic astrocytoma group it was ranged between 5 and 10% and the highest percentage of the positive cells was observed in glioblastoma cases and ranged from 10% even to 30%. The most evident MIB-1 expression was observed in the cells surrounding the pathological blood vessels and necrosis. Conclusions The high incidence of survivin and caspase-3 expression in diffuse and anaplastic astrocytoma cases may suggest, that the regulation between pro- and antiapoptotic proteins may play an important role in tumor growth and progression. The overexpression of survivin and MIB-1 expression in glioblastoma cases also may confirm the theory about the important role of anti-apoptotic and proliferation processes in glioblastoma progression and as such may be potential therapeutic targets.

      PubDate: 2016-02-13T14:26:29Z
      DOI: 10.1016/j.advms.2016.02.001
  • Recent advancements in diagnostic tools in mitochondrial energy metabolism
    • Authors: Naazneen Khan
      Pages: 244 - 248
      Abstract: Publication date: Available online 17 February 2016
      Source:Advances in Medical Sciences
      Author(s): Naazneen Khan
      The involvement of mitochondrial energy metabolism in human disease ranges from rare monogenic disease to common diseases and aging with a genetic and/or lifestyle/environmental cause. This wide ranging involvement is due to the central role played by mitochondrion in cellular metabolism, its role in cellular perception of threats and its role in effecting responses to these threats. Investigating mitochondrial function/dysfunction or mitochondria-associated cell-biological responses have thus become a common finding where the pathogenic processes are investigated. Although, such investigations are warranted, it is not always clear if mitochondria can indeed be associated with cause or merely playing a responsive role in disease pathology. As this key question is also essential to disease progression and therapy, it should be recognized in investigative design. We herewith, present an overview of the current approaches and technologies used and the practicalities around these technologies.

      PubDate: 2016-02-22T12:53:52Z
      DOI: 10.1016/j.advms.2016.02.002
  • Soluble CD163 modulates cytokine production by peripheral blood
           mononuclear cells stimulated by Dermatophagoides pteronyssinus allergens
           in vitro
    • Authors: Pawel Bernatowicz; Krzysztof Kowal
      Pages: 249 - 254
      Abstract: Publication date: Available online 8 March 2016
      Source:Advances in Medical Sciences
      Author(s): Pawel Bernatowicz, Krzysztof Kowal
      Purpose The CD163 is a scavenger receptor expressed exclusively on monocytes/macrophages which has been shown to exert anti-inflammatory effects. The aim of this study was to evaluate the effect of exogenous sCD163 on production of selected cytokines by peripheral blood mononuclear cells (PBMC) of house dust mite allergic patients (AAPs) stimulated in vitro with Dermatophagoides pteronyssinus (Dp) allergens. Patients and methods The study was performed in 24 AAPs and 12 healthy control subjects (HCs). Peripheral blood mononuclear cells were cultured for up to 144h (T144) in the presence of Dp extract with or without sCD163. Concentration of interleukin (IL) – 10, IL-13 and transforming growth factor beta (TGF-β) was evaluated in the cell culture supernatants using ELISA. Expression of the selected cytokines was evaluated in cell culture lysates using Taqman-based real time polymerase chain reaction (RT-PCR). Results Dp-stimulated PBMC from AAPs released more IL-10 and IL-13 than those from HCs. The greatest up-regulation of IL-10 expression was seen at T6, while that of IL-13 was delayed. Soluble CD163 augmented production of IL-10 in response to Dp stimulation. No significant effect of sCD163 on production of IL-13 and IL-10 by PBMC of HCs could be demonstrated. Conclusions In AAPs sCD163 modulates the immune response to Dp allergens potentiating anti-inflammatory, homeostatic mechanisms.

      PubDate: 2016-03-09T16:44:01Z
      DOI: 10.1016/j.advms.2016.02.004
  • Borrelia miyamotoi: A human tick-borne relapsing fever spirochete in
           Europe and its potential impact on public health
    • Authors: Edward Siński; Renata Welc-Falęciak; Joanna Zajkowska
      Pages: 255 - 260
      Abstract: Publication date: Available online 21 March 2016
      Source:Advances in Medical Sciences
      Author(s): Edward Siński, Renata Welc-Falęciak, Joanna Zajkowska
      Borrelia miyamotoi is a tick-borne bacterium which has only recently been identified in Europe as a human pathogen causing relapsing fever and little is known about its local impact on human health. There are three types of B. miyamotoi: Asian (Siberian), European, and American. B. miyamotoi is transmitted by the same Ixodes ricinus-persulcatus species complex, which also transmits B. burgdorferi s.l., the Lyme borreliosis group. Both Borrelia groups are mostly maintained in natural rodent populations. The aim of this review is to summarize the available literature on B. miyamotoi, with the focus of attention falling on Europe, as well as to describe its presence in ticks, reservoir hosts, and humans and discuss its potential impact on public health.

      PubDate: 2016-03-21T22:49:49Z
      DOI: 10.1016/j.advms.2016.03.001
  • Assessment of sleep disorders among patients with hypertension and
           coexisting metabolic syndrome
    • Authors: Grzegorz Kiełbasa; Katarzyna Stolarz-Skrzypek; Artur Pawlik; Monika Łątka; Tomasz Drożdż; Marta Olszewska; Agata Franczyk; Danuta Czarnecka
      Pages: 261 - 268
      Abstract: Publication date: Available online 28 March 2016
      Source:Advances in Medical Sciences
      Author(s): Grzegorz Kiełbasa, Katarzyna Stolarz-Skrzypek, Artur Pawlik, Monika Łątka, Tomasz Drożdż, Marta Olszewska, Agata Franczyk, Danuta Czarnecka
      Purpose The coexistence of hypertension, abdominal obesity, and carbohydrate and lipid metabolism disturbances favors the acceleration of other disease entities – also apart from cardiovascular system disorders. This study was planned to estimate the relationship between the presence of metabolic syndrome and sleep disorders among a group of hypertensive patients. Patients/methods The study was conducted on unselected group of 261 patients from the Outpatient Hypertension Clinic. The standardized questionnaire was used to collect the data on medical history, prescribed drugs and lifestyle. Sleep disorders were diagnosed based on the Athens Insomnia Scale and STOP-Bang Questionnaire. According to the study protocol, anthropometric parameters (weight, height, waist and neck circumferences), blood pressure, serum lipids and fasting glucose laboratory measurements were performed. Metabolic syndrome was defined according to IDF criteria. Results Sleep disorders were recognized in 183 participants of the study – significantly more frequently (P =0.03) among patients with diagnosed metabolic syndrome (120; 75%) in comparison with participants without this disturbance (63; 62.38%). Male gender, poor financial situation, tingling sensations before falling asleep and combination therapy of hypertension significantly increased the risk of sleep disorder occurrence in the group of patients with metabolic syndrome. Night food intake, aging, hypertension combination therapy and poor socioeconomic status were the factors increasing the risk of sleep problems among participants without metabolic syndrome. Conclusions Sleep disorders often coexist with metabolic syndrome in hypertensive patients. A number of factors increase the probability of occurrence of sleep disorders, but most of them are modifiable.

      PubDate: 2016-03-30T23:25:31Z
      DOI: 10.1016/j.advms.2016.03.005
  • Osteoprotegerin and osteoprotegerin/TRAIL ratio are associated with
           cardiovascular dysfunction and mortality among patients with renal failure
    • Authors: Marek Kuźniewski; Danuta Fedak; Paulina Dumnicka; Ewa Stępień; Beata Kuśnierz-Cabala; Marcin Cwynar; Władysław Sułowicz
      Pages: 269 - 275
      Abstract: Publication date: Available online 21 March 2016
      Source:Advances in Medical Sciences
      Author(s): Marek Kuźniewski, Danuta Fedak, Paulina Dumnicka, Ewa Stępień, Beata Kuśnierz-Cabala, Marcin Cwynar, Władysław Sułowicz
      Purpose The high prevalence of cardiovascular morbidity and mortality among patients with chronic kidney disease (CKD) is observed especially in those undergoing dialysis. Osteoprotegerin (OPG) and its ligands, receptor activator of nuclear factor kappa-B ligand (RANKL) and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) have been associated with cardiovascular complications. Our aim was to study their role as cardiovascular risk factors in stage 5 CKD patients. Patients and methods OPG, RANKL and TRAIL concentrations were measured in 69 hemodialyzed CKD patients and 35 healthy volunteers. In CKD patients, cardiovascular dysfunction was assessed with aortic pulse wave velocity (AoPWV), carotid artery intima-media thickness (CCA-IMT), coronary artery calcium score (CACS) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) serum concentrations. Cardiovascular and overall mortality data were collected during a 7-years follow-up. Results OPG plasma concentrations were higher in CKD patients comparing to controls. Total soluble RANKL was lower and OPG/RANKL ratio higher in patients. Soluble TRAIL concentrations did not differ between the groups and OPG/TRAIL ratio was higher in CKD patients. OPG and OPG/TRAIL positively predicted long-term mortality (all-cause and cardiovascular) in CKD patients. OPG positively correlated with AoPWV, CCA-IMT and NT-proBNP whereas OPG/TRAIL with AoPWV and NT-proBNP. Described relationships were independent of classical and non-classical cardiovascular risk factors, with exception of age. Conclusions Our study confirmed the role of OPG as a biomarker of cardiovascular dysfunction and a predictor of mortality in stage 5 CKD. OPG/TRAIL ratio can be proposed as a predictor of cardiovascular dysfunction and mortality.

      PubDate: 2016-03-21T22:49:49Z
      DOI: 10.1016/j.advms.2016.03.003
  • Relationship of the rs1799752 polymorphism of the angiotensin-converting
           enzyme gene and the rs699 polymorphism of the angiotensinogen gene to the
           process of in-stent restenosis in a population of Polish patients with
           stable coronary artery disease
    • Authors: Tadeusz Osadnik; Joanna Katarzyna Strzelczyk; Martyna Fronczek; Kamil Bujak; Rafał Reguła; Małgorzata Gonera; Marcin Gawlita; Anna Kurek; Jarosław Wasilewski; Andrzej Lekston; Marek Gierlotka; Michał Hawranek; Zofia Ostrowska; Andrzej Wiczkowski; Lech Poloński; Mariusz Gąsior
      Pages: 276 - 281
      Abstract: Publication date: Available online 28 March 2016
      Source:Advances in Medical Sciences
      Author(s): Tadeusz Osadnik, Joanna Katarzyna Strzelczyk, Martyna Fronczek, Kamil Bujak, Rafał Reguła, Małgorzata Gonera, Marcin Gawlita, Anna Kurek, Jarosław Wasilewski, Andrzej Lekston, Marek Gierlotka, Michał Hawranek, Zofia Ostrowska, Andrzej Wiczkowski, Lech Poloński, Mariusz Gąsior
      Purpose The renin–angiotensin–aldosterone system may influence in-stent restenosis (ISR) via angiotensin II, which stimulates the production of growth factors for smooth muscle cells. The aim of this work is to assess the influence of the rs1799752 polymorphism of the angiotensin-converting enzyme (ACE) gene and the rs699 polymorphism of the angiotensinogen (AGT) gene on the ISR in Polish patients with stable coronary artery disease (SCAD) who underwent stent implantation. Material/methods Two hundred and sixty-five patients with SCAD were included in the study. All patients underwent stent implantation upon admission to the hospital and had subsequent coronary angiography performed. The patients were divided into two groups – those with significant ISR (n =53) and those without ISR (n =212). The ACE polymorphism was assessed using the classical PCR method and the AGT polymorphism was determined using the TaqMan method for SNP genotyping. Results No difference in the frequency of angiographically significant ISR occurrence associated with the different ACE and AGT gene polymorphisms was observed. In a multivariable analysis, after correction for clinical variables, the relationship between the ACE and AGT genotypes within the scope of the analyzed polymorphisms and the process of restenosis was not found using a dominant, recessive and log-additive model. Late lumen loss was also independent of the genotypes of the polymorphisms before and after correction with angiographic variables. Conclusions The rs1799752 polymorphism and the rs699 polymorphism had no relationship with the occurrence of angiographically significant ISR and late lumen loss in a group of Polish patients who underwent metal stent implantation.

      PubDate: 2016-03-30T23:25:31Z
      DOI: 10.1016/j.advms.2016.03.006
  • Salusins and adropin: New peptides potentially involved in lipid
           metabolism and atherosclerosis
    • Authors: Leszek Niepolski; Alicja E. Grzegorzewska
      Pages: 282 - 287
      Abstract: Publication date: Available online 3 April 2016
      Source:Advances in Medical Sciences
      Author(s): Leszek Niepolski, Alicja E. Grzegorzewska
      Dyslipidemia is one of the most potent risk factors for the development of atherosclerosis. The high atherosclerotic risk in dyslipidemic patients is associated with endothelial dysfunction. During the last two decades, novel bioactive peptides have emerged as potential biomarkers of endothelial dysfunction and dyslipidemia-salusins and adropin. Salusin-alpha is likely to prevent atherosclerosis, while salusin-beta may act as a potential proatherogenic factor. Adropin was recently identified as important for energy homeostasis and lipid metabolism. Adropin is closely related to the inhibition of atherosclerosis by up-regulation of the endothelial nitric oxide synthase expression through the vascular endothelial growth factor receptor-2. These peptides represent a novel target to limit diseases characterized by endothelial dysfunction and may form the basis for the development of new therapeutic agents for treating metabolic disorders associated with atherosclerosis.

      PubDate: 2016-04-04T11:38:46Z
      DOI: 10.1016/j.advms.2016.03.007
  • New potential biomarkers of acetaminophen-induced hepatotoxicity
    • Authors: Katarzyna Siemionow; Joanna Teul; Paweł Drągowski; Jerzy Pałka; Wojciech Miltyk
      Pages: 325 - 330
      Abstract: Publication date: Available online 30 May 2016
      Source:Advances in Medical Sciences
      Author(s): Katarzyna Siemionow, Joanna Teul, Paweł Drągowski, Jerzy Pałka, Wojciech Miltyk
      Acetaminophen (APAP) is one of the most common antipyretic and analgesic drugs. Despite various precautions patients use APAP in amounts exceeding acceptable daily doses. APAP overdosing contributes to APAP intoxication, which leads to acute liver injury or necessity of exigent liver transplantation. Biomarkers that can be helpful in early diagnosis of liver injury during APAP overdosing are studied worldwide. This review presents recent reports on new potential biomarkers and their prospective application in clinical practice.

      PubDate: 2016-05-31T12:46:56Z
      DOI: 10.1016/j.advms.2016.05.001
  • The role of invasive diagnostics and its impact on the treatment of
           dilated cardiomyopathy: A systematic review
    • Authors: Katarzyna E. Gil; Agnieszka Pawlak; Robert J. Gil; Małgorzata Frontczak-Baniewicz; Jacek Bil
      Pages: 331 - 343
      Abstract: Publication date: Available online 2 August 2016
      Source:Advances in Medical Sciences
      Author(s): Katarzyna E. Gil, Agnieszka Pawlak, Robert J. Gil, Małgorzata Frontczak-Baniewicz, Jacek Bil
      Background Dilated cardiomyopathy is one of the most frequent causes of non-ischemic heart failure. Many factors including genetic disorders, infectious agents, toxins, drugs and autoimmune disorders might take part in the development of dilated cardiomyopathy. Diagnosis of left ventricular dilatation is most often limited to performing echocardiography and excluding ischemic etiology (coronary angiography). Since many pathologies take place at the cellular and subcellular level the only way to clarify the etiology of the disease is to examine the myocardium itself (endomyocardial biopsy). Methods A systematic literature search was conducted for studies published between September 2000 and September 2015 using the PubMed database. Results Of 7104 studies identified, 73 studies were included in this review. Controversies raised by opponents of the endomyocardial biopsy collide with the low percentage of serious complications confirmed in several single-center registries. Based on the available data the overall complication rate varies from 1% to about 3%, with 0.5% risk of serious complications. According to the current recommendations of the European and American scientific societies endomyocardial biopsy should be performed in most cases of left ventricular dilatation and heart failure of non-ischemic etiology. Endomyocardial biopsy allows for making the diagnosis and providing prognostic information especially in patients with familial dilated cardiomyopathy, diabetic cardiomyopathy with dilated phenotype, alcoholic cardiomyopathy, peripartum cardiomyopathy, iron overload cardiomyopathy, as well as inflammatory and viral cardiomyopathy. Iron overload cardiomyopathy, peripartum cardiomyopathy, inflammatory and viral cardiomyopathy are potentially treatable and reversible. Conclusions Targeted therapies are more effective when started early before myocardial injury becomes irreversible. Unfortunately, non-invasive techniques are not precise enough to decide if and which targeted therapy is required. Therefore endomyocardial biopsy should be mainly recognized as the essential diagnostic tool and should not be postponed.

      PubDate: 2016-08-10T21:21:57Z
      DOI: 10.1016/j.advms.2016.07.001
  • A comparative study of nuclear 8-hydroxyguanosine expression in Autoimmune
           Thyroid Diseases and Papillary Thyroid Carcinoma and its relationship with
           p53, Bcl-2 and Ki-67 cancer related proteins
    • Authors: Malek Mseddi; Riadh Ben Mansour; Naourez Gouiia; Fatma Mnif; Rabï Bousselaa; Mohamed Abid; Tahia Boudaouara; Hamadi Attia; Saloua Lassoued
      Abstract: Publication date: Available online 22 June 2016
      Source:Advances in Medical Sciences
      Author(s): Malek Mseddi, Riadh Ben Mansour, Naourez Gouiia, Fatma Mnif, Rabï Bousselaa, Mohamed Abid, Tahia Boudaouara, Hamadi Attia, Saloua Lassoued
      Purpose To investigate whether the oxidative stress is involved in the evolution of Graves’ disease (GD) and Hashimoto thyroiditis (HT) into Papillary Thyroid Carcinoma (PTC), 8-hydroxy-2′-deoxyguanosine (8-OHdG) and cancer related proteins (Bcl-2, p53 and Ki-67) expressions were evaluated in these pathologies. Patients and methods Immunohistochemical method was applied on 25 thyroid tissues. Allred score (AS) serving to evaluate the immunostaining is based on a scale from 0 to 8. “Negligible expression” was assigned to a score of 0 to 2, “expression” and “overexpression” were attributed to a score of 3–5 and ≥6 respectively. Results PTC cancer cells exhibited 100% 8-OHdG “overexpression” compared to 87.5% in PTC non-malignant epithelial (NME) ones (p <0.05). Higher 8-OHdG AS was found in PTC NME cells compared to GD and HT (p <0.001, p <0.05 respectively). “Overexpression” of Bcl-2 was noted in all PTC cell types. Remarkably, just like the PTC cancer and NME cells 33.3% of HT and 50% of GD patients’ revealed simultaneous “overexpression” of Bcl-2 and 8-OHdG in epithelial cells. No staining was detected for p53 in all pathologies. PTC lymphoid cells exhibited 100% “overexpression” for 8-OHdG and Bcl-2 with concomitant “negligible expression” for Ki-67 in 87.5% of patients. In contrast, HT lymphoid cells showed 22.2% “expression” and GD 62.5% “expression” and 12.5% “overexpression” of Ki-67. Conclusions Simultaneous “overexpression” of 8-OHdG and Bcl-2 in GD and HT could be considered as prognostic markers while “negligible expression” of Ki-67 in PTC lymphoid cells suggests an anergic state favoring the tumor escapes from the immune system.

      PubDate: 2016-06-24T22:58:54Z
      DOI: 10.1016/j.advms.2016.06.003
  • The strengths and weaknesses of non-invasive parameters obtained by
           echocardiography and cardiopulmonary exercise testing in comparison with
           the hemodynamic assessment by the right heart catheterization in patients
           with pulmonary hypertension
    • Authors: Katarzyna Ptaszyńska-Kopczyńska; Anna Krentowska; Emilia Sawicka; Anna Skoneczny; Małgorzata Jasiewicz; Małgorzata Knapp; Włodzimierz J. Musiał; Bożena Sobkowicz; Karol A. Kamiński
      Abstract: Publication date: Available online 14 June 2016
      Source:Advances in Medical Sciences
      Author(s): Katarzyna Ptaszyńska-Kopczyńska, Anna Krentowska, Emilia Sawicka, Anna Skoneczny, Małgorzata Jasiewicz, Małgorzata Knapp, Włodzimierz J. Musiał, Bożena Sobkowicz, Karol A. Kamiński
      Purpose Pulmonary hypertension (PH) diagnosis requires invasive assessment by right heart catheterization (RHC), but screening and monitoring are performed using non-invasive methods: echocardiography and cardiopulmonary exercise testing (CPET). The aim of the study was to assess correlations between the parameters obtained in non-invasive testing and RHC in patients with PH of different etiologies. Material/methods The study included 53 medical records of PH patients (32 women) aged 29–81 years. We analyzed correlations between RHC (systolic pulmonary artery pressure (sPAP), diastolic pulmonary artery pressure (dPAP), pulmonary vascular resistance (PVR), cardiac output (CO)) and echocardiographic (tricuspid annular plane systolic excursion (TAPSE), sPAP) and CPET parameters (end-tidal oxygen and carbon dioxide pressures (PetO2, PetCO2), ventilation efficiency (VE/VCO2) slope). Results Echocardiographic estimation correlated well with RHC measurement of sPAP (r =0.65, P <0.001). TAPSE correlated with PVR assessed with thermodilution method (r =−0.5, P =0.005), dPAP (r =−0.53, P =0.002) and CO (r =0.53, P =0.002). PVR assessed with thermodilution and Fick methods showed positive correlation with PetO2 (r =0.74, P <0.001 and r =0.72, P <0.001) and negative correlation with PetCO2 (r =−0.59, P =0.004 and r =−0.64, P =0.002) at the anaerobic threshold. VE/VCO2 slope correlated with dPAP (r =0.43, P =0.04) and PVR calculated with both methods (r =0.52, P =0.01 and r =0.52, P =0.02). Conclusions Simple cardiac function indicators obtained by commonly used non-invasive methods allow only approximate estimation of the main hemodynamic RHC-derived parameters: sPAP, CO and PVR. Obtained results suggest the relationship between RV dysfunction and ventilation abnormalities in PH patients.

      PubDate: 2016-06-16T06:01:43Z
      DOI: 10.1016/j.advms.2016.06.001
  • Can the sensitivity of the histopathological diagnosis of coeliac disease
           be increased and can treatment progression be monitored using mathematical
           modelling of histological sections' – A pilot study
    • Authors: Richard P.G. Charlesworth; Nicholas M. Andronicos; David R. Scott; James R. McFarlane; Linda L. Agnew
      Abstract: Publication date: Available online 14 June 2016
      Source:Advances in Medical Sciences
      Author(s): Richard P.G. Charlesworth, Nicholas M. Andronicos, David R. Scott, James R. McFarlane, Linda L. Agnew
      Purpose The aim of this pilot study was to attempt to define a set of equations from histological observations of tissue affected with coeliac disease (CD) to predict Marsh score. Material/methods Tissue from 15 patients with untreated CD, 6 patients with treated CD and 9 healthy control patients were stained using the standard H&E, Giemsa's staining for tissue sections and Alcian Blue protocols. A number of histological measures were then taken from each section and the data was used to ultimately design a set of linear predictive algorithms to calculate Marsh score. Results Using MANOVA and discriminant analysis, two linear functions were defined which could accurately predict the Marsh score of patients 62.5% (full Marsh score) to 79.2% (simplified Marsh score) of the time. Conclusions This pilot study has shown that a set of objective histological measures can be used to define algorithms to predict Marsh score in CD patients and also monitor treatment compliance and progression.

      PubDate: 2016-06-16T06:01:43Z
      DOI: 10.1016/j.advms.2016.06.002
  • NT-proCNP as a new indicator of asthenozoospermia
    • Authors: Ryszard Tomasiuk; Ricardo Faundez; Marek Cacko; Małgorzata Mikaszewska-Sokolewicz; Andrzej Cacko; Michał Rabijewski
      Abstract: Publication date: Available online 13 May 2016
      Source:Advances in Medical Sciences
      Author(s): Ryszard Tomasiuk, Ricardo Faundez, Marek Cacko, Małgorzata Mikaszewska-Sokolewicz, Andrzej Cacko, Michał Rabijewski
      Purpose C-type natriuretic peptide (CNP) is a proinflammatory peptide. The highest concentration of CNP is found in male reproductive organs. This study aims to analyze the role of N-terminal C-type natriuretic propeptide (NT-proCNP) as a new indicator of asthenozoospermia. Material/methods Semen was collected after 3–5 days of sexual abstinence from 86 men. The participants were between 25 and 38 years old, 51 of which had asthenozoospermia and 35 of which had normal sperm motility. Semen was analyzed for the concentrations of NT-proCNP, spermatozoa, percentages of live sperm, and sperm exhibiting a specific type of movement. The laboratory tests and analyses were performed using accepted methods and under appropriate conditions. Results A significant difference in the concentration and motility of spermatozoa was observed between the focus and control groups. The concentration of spermatozoa in the focus group was significantly lower than in the control group (median: 38.5 vs. 69.8mln/ml [p =0.016] respectively). The progressive motility of spermatozoa demonstrated a significantly lower performance in the focus group than in the control group (median: 10.4% vs. 45% respectively). The concentration of NT-proCNP was significantly higher in the focus group (median: 29.1 vs. 17.9pmol/l; p <0.001). The Area under the Receiver Operating Characteristic (AUROC) curve for the concentration of NT-proCNP – as an indicator of asthenozoospermia – was 0.733. Participants with a concentration of NT-proCNP higher than 28.8pmol/l had asthenozoospermia with 52.9% sensitivity and 94.3% specificity. Conclusions NT-proCNP – an indicator of inflammatory reaction – should be evaluated as an indicator of asthenozoospermia.

      PubDate: 2016-05-16T12:32:00Z
      DOI: 10.1016/j.advms.2016.04.002
  • Down-regulation of anti-apoptotic genes in tumor cell lines is facilitated
           by suppression of OCT4B1
    • Authors: Mohammad Reza Mirzaei; Mehdi Mahmoodi; Gholamhossein Hassanshahi; Zahra Ahmadi
      Abstract: Publication date: Available online 10 May 2016
      Source:Advances in Medical Sciences
      Author(s): Mohammad Reza Mirzaei, Mehdi Mahmoodi, Gholamhossein Hassanshahi, Zahra Ahmadi
      Purpose The OCT4B1 as a variant of OCT4 is expressed in both cancer cells and tissues. The anti-apoptotic property of this variant aid cancer cells to escape from apoptosis. Therefore, the aim of the present study was to determine the effects of OCT4B1 suppression on regulation of 25 genes involved in anti-apoptotic pathway in tumor cell lines. Material and methods AGS (gastric adenocarcinoma), 5637 (bladder tumor) and U-87MG (brain tumor) cells were transfected with specific OCT4B1 siRNA and a scramble siRNA by siRNA silencing gene technology, using Lipofectamine 2000 commercial kit. The real-time PCR technique was employed to examine and calculate fold changes of evaluated genes using the 2−ΔΔCT formula. Results Present results demonstrated that 22(88%) of interested genes were similarly down-regulated in all three examined cell lines. Our results also indicated that three genes (CASP2, IGF1R,TNF) were up-regulated. The CFLAR gene was down-regulated in AGS, while it was inversely up-regulated in 5637 and U87MG cells. Conclusions It may possibly be concluded that suppression of OCT4B1 can lead to apoptosis in tumor cell lines and this is at least facilitated via down-regulation of examined anti-apoptotic genes. Accordingly, suppression of OCT4B1 may probably be considered as useful tool in cancer therapy and research.

      PubDate: 2016-05-11T12:27:32Z
      DOI: 10.1016/j.advms.2016.04.004
  • Myriocin treatment affects lipid metabolism in skeletal muscles of rats
           with streptozotocin-induced type 1 diabetes
    • Authors: Krzysztof Kurek; Marta Garbowska; Dominika M. Ziembicka; Bartłomiej Łukaszuk; Jakub Rogowski; Adrian Chabowski; Jan Górski; Małgorzata Żendzian-Piotrowska
      Abstract: Publication date: Available online 9 May 2016
      Source:Advances in Medical Sciences
      Author(s): Krzysztof Kurek, Marta Garbowska, Dominika M. Ziembicka, Bartłomiej Łukaszuk, Jakub Rogowski, Adrian Chabowski, Jan Górski, Małgorzata Żendzian-Piotrowska
      Purpose The aim of this work was to assess the effect(s) of de novo ceramide synthesis inhibition on lipid metabolism in skeletal muscle tissue of type 1 diabetic rats. The latter seems to be of vital importance, since previous works have shown its positive influence on lipid metabolism and glucose homeostasis in the case of its counterpart – type 2 diabetes. Materials/methods The animals were randomly assigned to one of the following groups: C – control, M – myriocin (ceramide de novo synthesis inhibitor), D – diabetes (induced by streptozotocin injections); D+M – diabetes+myriocin. We have evaluated intracellular concentration of key sphingolipid species, via chromatography (GC and HPLC), and the activity of their most important enzymes, using radiometric approach. The aforementioned assessments were evaluated in respect to the three different types of muscle tissue representing different spectra of muscle metabolism (soleus – oxidative, red gastrocnemious – oxidative-glycolytic, white gastrocnemious – glycolytic). Results Interestingly, our therapeutic intervention not only lowered the level of ceramide, its precursors (sphinganine) and derivatives (sphingosine and sphingosine-1-phosphate), but also reduced other lipid species (triacylglycerols, diacylglycerols and free fatty acids) content, thus improving glucose homeostasis in type 1 diabetic animals. Conclusions In the light of the results ensuing from this study, it seems conceivable that the reduction of intramuscular ceramide production and accumulation could bestow an insulin-sensitizing effect. If so, then SPT inhibition could find potential future applications as a therapeutic intervention aimed to mitigate the effects of insulin resistance.

      PubDate: 2016-05-11T12:27:32Z
      DOI: 10.1016/j.advms.2016.04.003
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