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Journal Cover
International Journal of Biochemistry and Biophysics
Number of Followers: 1  

  This is an Open Access Journal Open Access journal
ISSN (Print) 2331-9925 - ISSN (Online) 2331-9933
Published by Horizon Research Publishing Homepage  [54 journals]
  • Concentrations of Fat-soluble Vitamins of Trema guineensis Aqueous and
           Ethanolic Extracts and Rats Supplemented

    • Abstract: Publication date:  Jan 2018
      Source:International Journal of Biochemistry and Biophysics  Volume  6  Number  1  Droucoula Guillaume Cyril   Tra Bi Irié Otis   Miezan Bile Aka Patrice   Kouakou Sylvain Landry   Bla Kouakou Brice   and Yapi Houphouët Félix   This study was undertaken to determine vitamins A, D and E composition of aqueous and ethanolic extracts of Trema guineensis and effect of supplementation of these extracts on serum concentrations of these vitamins. Aqueous and ethanolic extracts of Trema guineensis were obtained by decoction and maceration of leaves powder, respectively. Then, they were administered to animals at doses of 100 and 200 mg/kg body weight against a control group treated with distilled water for two weeks. Composition of vitamins in extracts and serum concentrations of vitamins A, D and E were performed by HPLC. Results showed that Trema guineensis extracts contained high concentrations of vitamins A, D and E. Thus, vitamins A and D serum concentrations were weakly influenced while that of vitamin E increased strongly during administration of Trema guineensis extracts. This study therefore showed that extracts of Trema guineensis had vitamins A, D and E and a beneficial effect on serum concentrations of these vitamins.
      PubDate: Jan 2018
       
  • In vitro Investigation of the Anti-sickling and Erythrocyte Membrane
           Stabilizing Potentials of Elaesis guineensis Jacq Flower

    • Abstract: Publication date:  Jan 2018
      Source:International Journal of Biochemistry and Biophysics  Volume  6  Number  1  Florence Ezewanyi Ogwutum   Augustine Amadikwa Uwakwe   Mathew Owhonda Wegwu   and Justice Obinna Osuoha   Anti-sickling activity of Elaeis guineensis jacq flowers was investigated to determine the capability of the plant to inhibit the polymerization of sickle cell hemoglobin, maintain membrane osmotic fragility and recover the Fe2+/Fe3+ ratio. Spectrophotometric technique was employed to determine the rate of sodium metabisulphite provoked HbSS erythrocytes polymerization. The profile for HbSS erythrocytes polymerization of test and control specimens demonstrated growing level of polymerization at three investigational concentrations (20mg/ml, 40mg/ml and 60mg/ml) while (80mg/ml, 100mg/ml, 120mg/ml) concentrations of the extracts showed pronounced anti-sickling activity through inhibition of HbSS gelation. The estimation of mean corpuscular fragility (MCF), which is the concentration of saline causing 50% hemolysis of the erythrocytes, revealed that the plant extract decreased the MCF values of the HbSS erythrocytes at all concentrations in comparison to the control. The Fe2+/Fe3+ investigation revealed an increase in the test groups when compared to the control. The aqueous extracts of Elaeis guineensis jacq flowers demonstrated towering potency in altering the polymerization of sickle cell hemoglobin at increased concentration, enhancement in Fe2+/Fe3+ ratio and maintaining erythrocyte membrane integrity.
      PubDate: Jan 2018
       
  • Fabrication and Characterization of Layered Graphene Oxide Biocompatible
           Nano-Film by Various Methods

    • Abstract: Publication date:  Jan 2018
      Source:International Journal of Biochemistry and Biophysics  Volume  6  Number  1  Himanshu Kharkwal   H.C. Joshi   and K.P. Singh   The present study includes preparation, fabrication and characterization of GO, GO coated PCTE and Egg membrane respectively. Characterization of GO was done by UV-Visible spectrophotometery, Fourier Transform Infrared spectroscopy (FT-IR), Energy Dispersive Spectroscopy (EDS), and Scanning Electron Microscopy (SEM). Different membranes i.e. PCTE (Polycarbonate track etch membrane) and egg membrane are used for the coating of graphene oxide. The egg membrane was isolated from the egg shell by dipping the egg in 8% HCl solution. These membrane i.e. PCTE and egg membrane were then fabricated with graphene oxide by drop casting method and vacuum filtration. The coated membrane was then characterized by different imaging technique such as FE-SEM, Inverted microscopy, FT-IR, and EDX. Based on the characterization best suited method for coating was determined and we found vacuum filtration method as the best method for coating. The simple (non-coated) and GO coated membrane of Egg and PCTE were then used for checking the potential gradient across the membrane by using digital multimeter and from this we came to conclusion that the potential gradient across the GO coated PCTE membrane is highest and lowest potential is of GO coated Egg membrane. Above all we found that GO coated egg membrane is highly beneficial and cost effective as compared to artificial membranes.
      PubDate: Jan 2018
       
  • Organic Pesticides Concentration in Water, Sediments and Fish Tissues
           obtained from Ogbakiri River in the Niger-Delta Region, Nigeria and Its
           Health Implication on Human Consumption

    • Abstract: Publication date:  Nov 2017
      Source:International Journal of Biochemistry and Biophysics  Volume  5  Number  4  Chinedu J. Okonkwo   Idongesit E. Archibong   Chioma J. Okereke   Justice O. Osuoha   and Uche C. Njoku   Organic pesticides concentrations in tissues of Chrysichthys nigrodigitatus (catfish) and Liza falcipinis (mullet) sampled from Ogbakiri River, Niger Delta, Nigeria were investigated to find out the extent of pesticide contamination in the river and its possible health implication on human consumption assessed. Pesticides were analyzed using gas chromatography coupled with electron capture detector (GC-ECD). The concentration of pesticides ranged from 0.0010±0.0001 to 0.0095±0.0002 µg/l and 0.0007±0.0001 to 0.0110±0.0002µg/g in water and sediments, respectively. Similarly, pesticides concentration in fish tissues ranged from 0.0002±0.0001 to 0.0185±0.0002µg/g and 0.0003±0.0001 to 0.0098±0.0002µg/g in liver and muscle respectively. Moreso, to assess health risk concerns, the measure concentrations of pesticide residues were compared with regulatory benchmarks. Risk hazard estimates of dichlorodiphenyltrichloroethanes, Chlordanes, hexachlorobenzene in the studied fishes showed no potential adverse effect to human consumption at the observed concentrations. The cancer risk was also evaluated according to the guideline as stipulated by USEPA and was within acceptable risk range of 10-4. Thus exposed populations are not at cancer risk through consumption of fish from this river.
      PubDate: Nov 2017
       
  • Simulating the Ideal Body Weight in Human Populations

    • Abstract: Publication date:  Nov 2017
      Source:International Journal of Biochemistry and Biophysics  Volume  5  Number  4  Md. Abdul Hakim   Estimation of ideal body weight in human samples in a fine located geographic level is vital for effective health promotion programmes, provision of better health services and population-specific health planning and management. Lack of nutritional knowledge and information gap on various health and nutritional tools negatively impacts the ability of local and national agencies to manage serious health issues and related risks in the community. A solution to this challenge would be to develop a method that simulates reliable statistics on assessing the ideal body weight of human populations. This paper provides a significant appraisal of the biophysical methodologies for estimating ideal body weight to mitigate health-related problems of populations at geographical limited areas. There is no procedure in this multidisciplinary area in estimating ideal body weights of human samples in health physics and biostatistics. The dietician is often at bay in the route ahead of perfect dieting to hold up nutritional soundness of the sample at a population in a community. The aim of this current study is making a dot over these ongoing perils simulating a biophysical modeling to be used in prescribing a confounding free diet. The study findings are the equations (7), (8) and (9) in different health plight regarding to age groups in human population can be an outstanding mathematical modeling as a dieting tool in nutritional physics applicable to the study on health and nutritional research and in the branch of biostatistics. Also it can be a uniquely functional biophysical modeling in the branch of health pedagogy in nutritional epidemiology.
      PubDate: Nov 2017
       
  • Evaluation of Doses-response and Combined Preventive Effects of Zinc and
           Vitamin D on Liver Toxicity Induced by Carbon Tetrachloride in Wistar Rats
           

    • Abstract: Publication date:  Jun 2017
      Source:International Journal of Biochemistry and Biophysics  Volume  5  Number  2  Serge D. Kone   Francis A. Yapo   Goue Gnahoue   and Felix H. Yapi   Severe hepatic insufficiency caused by a toxic is a strong danger to the individual survival. Zinc and vitamin D are micronutrients involved in the prevention of several diseases. Our study was to evaluate the potential preventive effects of these micronutrients in hepatic toxicity induced by carbon tetrachloride (CCl4) in rats. One hundred and fourteen (114) male rats were divided into 19 lots of 6 male rats each according to their body weight. Carbon tetrachloride (CCl4) was used to induce hepatic toxicity in rats. The rats were treated respectively with 25, 50 and 100 mg/kg of zinc and 6, 12 and 24μg/kg of Vitamin D and also with the combination of zinc and Vitamin D at the same concentrations. There was a significant increase in serum of ALT, AST without changing Total Protein level in the serum of rats treated with CCl4 compared to rats in the control lots. However, Zinc and Vitamin D supplementation caused a significant decrease in serum of ALT and AST activity without changing Total Protein concentration in the serum of rats. This study revealed that that zinc combined with Vitamin D could be a good protection against hepatic toxicity induced by chemicals.
      PubDate: Jun 2017
       
  • Study of Microalbuminuria and hs-CRP in Non-obese and Obese Normotensive
           and Hypertensive Males and Evaluation of Microalbuminuria and hs-CRP
           Changes according to the Stages of Hypertension & Grades of Hypertensive
           Retinal Changes

    • Abstract: Publication date:  Jun 2017
      Source:International Journal of Biochemistry and Biophysics  Volume  5  Number  2  A.G. Thivyah Prabha   Aims & Objectives: To measure Microalbuminuria and hs-CRP in obese and non-obese male hypertensive patients and control groups and to correlate their levels with various stages of hypertension and different grades of hypertensive retinal changes. Men have higher prevalence of hypertension (29.4%) than women (26.5%)[1] Materials & Methods: The study was conducted among 60 normotensive individuals and 60 hypertensive individuals over a period of 6 months on OPD basis. All individuals were males in the age group of 40-60 years. Blood samples were collected and estimation of plasma glucose, urea, creatinine, lipid profile, hs- CRP and Urinary microalbumin were done. Results: The mean value of microalbuminuria and hs- CRP were higher among obese hypertensives than obese controls and were statistically significant. The changes in the mean value of microalbuminuria and hs-CRP within the different stages of hypertension were highly significant. Similarly the correlation of microalbuminuria and hs-CRP within the different grades of hypertensive retinal changes were highly significant. Conclusion: The serum level of hs-CRP and urinary microalbuminuria were elevated in hypertensive patients. The increase of both hs- CRP and microalbuminuria were significantly higher in obese hypertensives than non-obese hypertensives. Microalbuminuria & hs CRP levels correlate with various grades of hypertensive retinal changes.
      PubDate: Jun 2017
       
  • Xenon-Water Interaction in Bacterial Suspensions as Studied by NMR

    • Abstract: Publication date:  Feb 2017
      Source:International Journal of Biochemistry and Biophysics  Volume  5  Number  1  Victor Rodin   Alexander Ponomarev   Maxim Gerasimov   and Leonid Gurevich   Xenon is a perspective gas for creation of oxygen free environment for different applications of biomaterials. To use xenon in suspensions and products properly it is necessary to know the molecular mechanisms of its interactions with water and cells. This work reports the study of bacterial suspensions of Escherichia coli in the presence of xenon using nuclear magnetic resonance (NMR). The work studied how the spin-lattice relaxation times of water protons in suspension change under xenon conditions. Xenon is able to form clathrate hydrates with water molecules at a temperature above the melting point of ice. The work studied NMR relaxation times which reflect the rotation freedom of water molecules in suspension. Lower relaxation times indicate reduced rotational freedom of water. Single exponential behavior of spin-lattice relaxation of protons in the suspensions of microorganisms has been registered. A recovery of longitudinal magnetization in cell suspensions with xenon clathrates has been characterized by two peaks in T1-distribution. Fast relaxing T1-component was related to the intracellular water and depended on the amount of xenon clathrates. The obtained results elucidate how the NMR method can monitor the process of clathrate formation and how the xenon atoms and hydrates interact with cells.
      PubDate: Feb 2017
       
  • Study of the in vitro Antibacterial Activity of the Aqueous and
           Hydroethanolic Extracts from the Leaves of the Erythrococca anomala
           (Euphorbiaceae) on Six Bacterial Strains

    • Abstract: Publication date:  Feb 2017
      Source:International Journal of Biochemistry and Biophysics  Volume  5  Number  1  Miezan Bilé Aka Patrice   Koffi Allali Eugene   BY Philemon   Okpekon Aboua Timothée   and Yapi Houphouët Felix   The problems of resistance of pathogenic bacteria to the common antibiotics in hospitals and the high costs of the treatments of pathologies caused by those resistant bacteria have brought about innovation in the search for alternative treatments: plants. The purpose of this study is to assess the antibacterial activities of the aqueous and hydroethanilic extracts from the leaves of the Erythrococca anomala on the in vitro growth of six multiresistant bacterial strains: Pseudomonas aeruginosa, Escherichia coli Klebsiella pneumoniae, Neisseria meningitidis, Streptococcus pneumoniae, Staphylococcus aureus, isolated from patients. The results of the studies showed that the aqueous extract, more active than the hydroethanolic extract, contains Minimal Inhibitory Concentrations (MICs) which vary between 0.78 mg/mL and 12.5 whereas the minimal bactericidal concentrations (MBCs) vary between 0.78 mg/mL and 25 mg/mL. Concerning the hydroethanolic extract, the MICs and the MBCs, vary between 3.125 mg/mL and 25 mg/mL each. Streptococcus pneumoniae and Staphylococcus aureus are more susceptible because they need 0.78 mg/mL and 1.56 mg/mL respectively before they are completely inhibited by the aqueous extract. Compared with the Ciprofloxacin, a pure molecule, those extracts have clearly better antibacterial activity. That may justify why those leaves are used in traditional areas as anti-infective treatment. Erythrocacca anomala is a glimmer of hope for the treatment of infectious diseases in Côte d'Ivoire.
      PubDate: Feb 2017
       
  • Unexpected Aftereffects of Low Dose γ-irradiation on Quality of
           Individual Dry Pea Seeds

    • Abstract: Publication date:  Feb 2017
      Source:International Journal of Biochemistry and Biophysics  Volume  5  Number  1  Tatiana V. Veselova   Vladimir A. Veselovsky   and Natalie V. Obroucheva   Time-dependent aftereffect induced by γ-radiation in dry pea seeds at non-lethal 0.19-10 Gy doses is considered. By using phosphorescence at room temperature (RTP), seed lot was fractionated to strong and weak seeds producing normal and abnormal seedlings, respectively, and to dead seeds. After 0.19 Gy γ-radiation, strong seeds transformed to weak seeds during two months, whereas seeds after 7-10 Gy became weak during 2-4 days that was accompanied by doubling glucose content and decreasing seed hydration. This indicated non-enzymatic hydrolysis of carbohydrates apparently caused by γ-induced activation of water molecules. At 7-10 Gy, an unexpected back transition of weak seeds to "improved" seeds occurred, producing normal seedlings. This "improvement" is due to glucose decrease to strong- seed level presumably via reaction between free glucose carbonyl and protein amino group. "Improvement" is transient; these seeds gradually die apparently due to end products of amino-carbonyl reaction (Amadori-Maillard products). Therefore, two effects of indirect non-target action of γ-radiation on dry seeds at low doses were suggested: non-enzymatic hydrolysis of carbohydrates and then amino-carbonyl reaction. They result at first in transition of strong to weak seeds and then of weak to "improved" seeds, response time being dependent on γ-radiation dose.
      PubDate: Feb 2017
       
  • Nutritional Effect of Boiled and Baked Soybean Dockounou on the Wistar
           Rats Health Conditions

    • Abstract: Publication date:  Feb 2017
      Source:International Journal of Biochemistry and Biophysics  Volume  5  Number  1  Kouadio N. Joseph   Kra K. A. Sevérin   and Niamké L. Sébastien   This study was to assess the physiological impact of boiled and baked soybean Dockounou on Wistar rats wealthy after feed them with these diets. For this, 15 strains Wistar rats aged 50 ± 3 days and with average weight between 55 and 60 g were fed with different diets. Biochemical parameters of their serum and weight of organs (liver and kidney) were performed at the end of experiment. The glycemia content registered in serum was 0.74 ± 0.13g/L and 0.74 ± 0.11g/L for boiled and baked soybean Dockounou, respectively. That of cholesterol was 0.91 ±0.16g/L and 0.86 ±0.15g/L and HDL was 0.65 ± 0.069g/L and 0.62 ± 0.05g/L for the same diets respectively. Any pathological, dysfunction and physiological abnormalities were not identified as far as concern rat's serum and organs. Thereby, any significant difference at 5% level was registered between the control diet results and those of soybean Dockounou. Thus, the consumption of boiled and baked Dockounou is benefit for Wistar rats' health and also these feed can be advised as a good diet for their use in laboratory.
      PubDate: Feb 2017
       
  • Homology Modeling, Molecular Dynamics Simulation and Essential Dynamics on
           Anopheles gambiae D7r1

    • Abstract: Publication date:  Aug 2017
      Source:International Journal of Biochemistry and Biophysics  Volume  5  Number  3  Chayanika Goswami   Manikandan Jayraman   Tamizhmathi Bakthavachalam   Guneswar Sethi   and Ramadas Krishna   The proteins produced by the saliva and salivary glands of blood sucking arthropods play a vital role in transmission of the infected parasite to host and interfere the parasitic life cycle. The structure prediction of D7r1 and its active site leads to inhibition of hemostasis and inflammation in the host. D7r1 is a member of D7-related (D7r) salivary gland proteins that interferes various aspects of host physiology. Considering the significance of a protein, three dimensional structure of D7r1 model was generated by homology modeling and validated by PROCHECK, ERRAT, Verify-3D, RAMPAGE and Q mean server. The predicted structure has 96.9% of residues in the most favored region of the Ramachandran plot. The sequence and structural alignment between D7r1 and template 2PQL reveals that similar active site residues such as Ile42, Arg43, Tyr45, His56, Met57, Val60, Phe131, and Met156 involved in binding pocket formation. Further, a molecular dynamics simulation study was performed to reveal the prolonged stability of D7r1 protein. The essential dynamics which include PCA and FEL analysis were used to evaluate the conformational stability of D7r1. This combined molecular dynamics simulation and essential dynamics were used to provide comprehensive information of D7r1 and its active site prediction gain insights into the development of novel lead molecules for disrupting host-seeking behavior of mosquitoes.
      PubDate: Aug 2017
       
  • Application of Nanopore of Solid Membrane for Recognition of fluorescent
           Pseudomonas

    • Abstract: Publication date:  Aug 2017
      Source:International Journal of Biochemistry and Biophysics  Volume  5  Number  3  H. C. Joshi   K. P. Singh   Abhishek Tomar   and Prashant Singh   The uses of biosensors for sensitive and specific detection of various analytes are of great importance, and its success is often dictated by the nature of the detection element (the specific ligand) and the choice of target analytes. The conventional agriculture caused considerable impacts on soils and waters. It is important to change certain agricultural managements to environmental cleaner techniques. The sustainable agriculture has pointed many approaches and techniques to reduce environmental impact. One of those strategies is the utilization of the soil microbiota to induce plant growth, control of plant diseases and biodegradation of xenobiotic compounds. Studies on the relationship between roots and microbiota are essential to achieve agricultural applications. These studies indicate that one of the most abundant microorganisms in the rhizosphere (area around the roots) is fluorescent Pseudomonas spp. They have been considered as an alternative to agrochemicals for controlling plant diseases and increasing plant development. In the present study gold coated polycarbonate membrane of pore size 100 nm was used. The coated membrane carries carboxylic, thiol and hydroxyl groups. Carboxylic groups are being activated by treating the coated nanoporous membrane with the NHS-EDC linker for the binding of antibodies against the pathogen (fluorescent Pseudomonas) to be detected. After the activation of the membrane, antibodies (which are generated against the fluorescent Pseudomonas) were immobilized over the membrane in the humid chamber. After leaving it for an hour, unbound antibodies were washed by Phosphate buffer Saline. The remaining sites on membrane were then blocked by the Bovine serum albumin. The membrane was then subjected for the detection of pathogen by the electrochemical immunosensor by observing the change in the impedance value. The characterization of such membrane was done by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS) and Thermo gravimetric Analyzer (TGA).
      PubDate: Aug 2017
       
 
 
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