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Jurnal Fitopatologi Indonesia
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  This is an Open Access Journal Open Access journal
ISSN (Print) 0215-7950 - ISSN (Online) 2339-2479
Published by Bogor Agricultural University Homepage  [24 journals]
  • Cover Jurnal Fitopatologi Vol. 14 No. 4, Mei 2018

    • Authors: Editor Jurnal Fitopatologi Indonesia
      Abstract:
      DOI : http://dx.doi.org/10.14692/jfi.14.4.i 
      PubDate: 2018-11-22
      Issue No: Vol. 14, No. 4 (2018)
       
  • Sebaran Nematoda Sista Kentang di Wonosobo dan Banjarnegara, Jawa Tengah

    • Authors: Dani Sutanta Syafii, Lisnawita Lisnawita, Hasanudin Hasanudin
      First page: 111
      Abstract: Distribution of Potato Cyst Nematode in Wonosobo and Banjarnegara, Central Java
      Potato cyst nematode (PCN), Globodera spp., is the primary pathogens that infect potato plants and has been distributed world wide. This study aimed to determine the geographic distribution and species of PCN that infect potato plants in Wonosobo and Banjarnegara, Central Java through a survey, morphological and molecular approaches. Morphological characters were observed among cyst (fenestra diameter, distance from anus to the fenestra, and the Graneks ratio, eggs (length and width of eggs), and the juvenile stage 2 (J2) (length and width of the body, the length and width of the head, the length of the stylet, knob type, as well as the length of the tail). While the molecular identification by polymerase chain reaction is based on the amplification of the internal area transcribed spacers using G. rostochiensis PITSr3 specific primer, G. pallida PITSp4 specific primer and universal nematode primer ITS5. Twelve isolates of PCN, i.e. 5 isolates from Wonosobo and 7 isolates from Banjarnegara were used in this study. The results showed that all isolates were identified as single species, G. rostochiensis. 

      PubDate: 2018-11-22
      Issue No: Vol. 14, No. 4 (2018)
       
  • Penggunaan Bakteri Kitinolitik sebagai Pengendali Hayati Colletotrichum
           capsici pada Tanaman Cabai

    • Authors: Dian Syahfitri, Nisa Rachmania Mubarik, Lisdar A Manaf
      First page: 120
      Abstract: Use of Chitinolytic Bacteria as Biological Control of Colletotrichum capsici on Chili Plants
      Colletotrichum capsici is known as the causal agent of anthracnose disease in chili plant and may cause reduction of crop yield. Chitinolytic bacteria, namely Serratia marcescens KAHN 15.12, Bacillus thuringiensis SAHA 12.12, and BAE 36 were reported to have antagonistic activity against C. capsici. Therefore, a study was conducted to determine the potential of chitinolytic bacteria on controlling C. capsici on chili plants in greenhouse experiment. Three bacterial isolates used as biocontrol agent was formulated by using talcum as carrier materials. The methodologies consisted of characterization of bacterial isolates, formulation of biocontrol agent, viability test of bacterial isolate, efficacy of biocontrol agents in the laboratory and in the greenhouse.  Disease severity in the laboratory reached 64% when chili treated with isolate formulation of BAE 36.  In the greenhouse, BAE 36 isolate formulation and consortium formulation were able to suppress infection of C. capsici; each was indicated by disease incidence of 25% and 50%, respectively. These results indicated that chitinolytic bacterial formulations could be potencial as biocontrol agents of C. capsici.
      PubDate: 2018-11-22
      Issue No: Vol. 14, No. 4 (2018)
       
  • Distribusi Nematoda Pucuk Putih Padi Aphelenchoides besseyi di Pulau Jawa

    • Authors: Didiet Rahayu Diana
      First page: 129
      Abstract: Distribution of Rice White Tip Nematode Aphelenchoides besseyi in Java Island
          Aphelenchoides besseyi is one of the most important rice seed borne nematode causing white tip disease in Indonesia.  The status of A. besseyi is considered as quarantine pest category A2, with limited distribution area in Java, Sumatera, and South Kalimantan). The aim of this research was to detect and identify A. besseyi from rice seed based on morphological, morphometry and molecular approaches and to determine its distribution in rice growing areas in Java. Rice seed samples, consisting of 26 varieties, were obtained from seed producers, seed distributors, and farmers in Java. Nematode extraction was done using Baermann method. Molecular identification was carried out by polymerase chain reaction and followed by DNA sequencing and nucleotide analysis. A. besseyi was detected from 74.32% of seed samples. A. besseyi was confirmed to be distributed in Lebak, Bogor, Sukabumi, Subang, Indramayu, Klaten, Sragen, Sukoharjo, Boyolali, Pati, Pemalang, Yogyakarta Sleman, Magetan, Blitar, Tuban, Gresik, Nganjuk, and Banyuwangi. Further nucleotide analysis showed that A. besseyi isolates from rice seed cv Ciherang (Yogyakarta) and cv IR 64 (Banyuwangi) have 99% homology to those from India, China, USA; and  98% homology to those from Taiwan.
      PubDate: 2018-11-22
      Issue No: Vol. 14, No. 4 (2018)
       
  • Ekstrak Tanaman Binahong sebagai Pengendali Penyakit Hawar Pelepah Daun
           Padi

    • Authors: Endah Yulia, Fitri Widiantini
      First page: 138
      Abstract: Binahong Extract for Controlling Sheath Blight Disease of Rice Paddy
      Sheath blight disease, caused by Rhizoctonia solani, is an important disease that can affect rice production. The disease is commonly controlled using synthetic fungicides. This study was aimed to test the potential of binahong plant (Anredera cordifolia) as botanical fungicide to inhibit the growth of R. solani and the development of blight disease on rice seedlings. The research was conducted in 2 stages, i.e. in vitro and rice seedling testing. In vitro test was carried out on PDA medium containing different concentrations of binahong leaf extract (0.125%, 0.25%, 0.5%, 1%, and 2%). Rice seedling testing was completed by soaking rice seeds in five concentrations of binahong leaf extract followed by inoculation of R. solani.  The experiments were arranged in a completely randomized design. The variables observed consist of inhibitory ability of binahong leaf extract, colony morphology of R. solani, and disease incidence. Binahong leaf extract inhibited 35.2% growth of R. solani colonies. Treatment of binahong leaf extract had some effects on mycelium of R. solani; macroscopically the mycelium was shorter, thinner and congregated, whereas microscopically it was smaller, elongated and tended to be colourless compared to control. The treatment of 2% binahong leaf extract on rice seeds decreased the incidence of seedling blight disease with the effectiveness reached 100%. Thus, binahong leaf extract has the potential to suppress sheath blight disease caused by R. solani.
      PubDate: 2018-11-22
      Issue No: Vol. 14, No. 4 (2018)
       
  • Uji In Vitro Asap Cair terhadap Ralstonia syzygii subsp. celebesensis
           Penyebab Penyakit Darah pada Pisang

    • Authors: Aisyah Imas, Giyanto Giyanto, Meity Suradji Sinaga, Abdjad Asih Nawangsih, Gustan Pari
      First page: 145
      Abstract: In Vitro Test of Liquid Smoke against Ralstonia syzygii subsp. Celebesensis, the Cause of Blood Disease in Bananas
       Blood disease, caused by Ralstonia syzygii subsp. celebesensis, is an important disease of banana plants in Indonesia. Several control methods have been done, but the result were not effective.  Liquid smoke from wood waste is reported to be antimicrobial, but its use for controlling R. syzygii subsp. celebesensis has never been reported. Research was conducted to examine the ability of liquid smoke produced from coconut shell (CS-LS), pinecone (P-LS), and oil palm branch (OPB-LS) in inhibiting the growth of R. syzygii subsp. celebensensis in vitro and its effect on bacterial cell morphology. Efficacy test of liquid smoke was carried out by agar diffusion method and measurement of bacterial density by spectrophotometry with ʎ 600 nm. Observation of bacterial cell morphology was carried out by electron microscopy. The CS-LS, P-LS, and OPB-LS starting from 0.5% concentration showed inhibitory activity against R. syzygii subsp. celebesensis both on triphenyl tetrazolium chloride medium and luria bertani broth. Liquid smoke also caused damage to cell walls and cell membranes. Therefore, liquid smoke has the potential to be used as component in control method for R. syzygii subsp. celebesensis.
      PubDate: 2018-11-22
      Issue No: Vol. 14, No. 4 (2018)
       
 
 
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