Publisher: Badan Penelitian dan Pengembangan Kesehatan   (Total: 6 journals)   [Sort by number of followers]

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ASPIRATOR : J. of Vector-borne Disease Studies     Open Access  
Buletin Penelitian Kesehatan     Open Access   (Followers: 2)
Health Science J. of Indonesia     Open Access   (Followers: 2)
Jurnal Kefarmasian Indonesia     Open Access   (Followers: 1)
Jurnal Vektor Penyakit     Open Access  
Media Penelitian dan Pengembangan Kesehatan     Open Access   (Followers: 1)
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Health Science Journal of Indonesia
Number of Followers: 2  

  This is an Open Access Journal Open Access journal
ISSN (Print) 2087-7021 - ISSN (Online) 2338-3437
Published by Badan Penelitian dan Pengembangan Kesehatan Homepage  [6 journals]
  • HIV-1 Gag specific IgG response in mice immunized with Vp22-Gag vaccine

    • Authors: Heri Setiyo Bekti
      Abstract: Background : Stimulation of Gag-specific CD8+ T-cell response, associated with reduction in viremia, viral replication control, and slow disease progression. Effective CD8+ T cell response is also influenced by CD4+ T cells. Gag recombinant protein may be cloned and expressed in the prokaryotic system and when they are immunized in experimental animals or human will have property as exogenous antigens. Exogenous antigens may become endogenous antigens by adding proteins that have the ability to translocate into the cell membrane, one of which is the Vp22 protein. Method : Transformation of recombinant plasmids in prokaryotic expression system with heat shock method was followed by expression of recombinant proteins. Purification of recombinant proteins was performed with affinity chromatography. The molecular weight analysis of recombinant proteins was performed with SDS-PAGE. Western blotting was performed to determine the reactivity of recombinant proteins with polyclonal antibodies against p24 antigens. Transfection of CHO cell and immunization of DDY mice with recombinant proteins was conducted to determine intracellular migration ability and stimulation of specific immune response. Results : Western blotting test, indicating recombinant protein may interact with polyclonal antibody against p24 antigens. The observation of a confocal microscope showed recombinant proteins localized with endosomes. The ELISA test indicates Gag-specific IgG response after immunization in DDY mice. Conclusion : Recombinant proteins may be expressed on a prokaryotic expression system. The ability of recombinant protein intracellular migration in CHO cell has not been proven. Recombinant proteins may stimulate Gag-specific IgG response. Keywords:   Gag and Vp22-Gag recombinant proteins; intracellular migration, Gag-specific IgG response.  
      PubDate: 2017-12-31
      Issue No: Vol. 8 (2017)
  • A Prediction Model of Dengue Incidence Using Climate Variability in
           Denpasar City

    • Authors: Khadijah Azhar, Rina Marina, Athena Anwar
      Abstract: Background: Denpasar city is one of cities with the highest dengue incidence in Indonesia. Environmental factors such as climate variability is one of the factors that influence the incidence of dengue. Methods: This study aimed to obtain a predictive dengue incidence models using secondary data of weekly climate and surveillance of dengue in Denpasar, Bali, 2010-2014. Climate data was obtainedfrom Indonesia Agency for Meteorological, Climatological, and Geophysical  (BMKG), while   dengue cases were obtained from Early Warning Alerts Respons System (EWARS), the Indonesian Ministry of Health.  Data analysiswas using linear regression with various combinations of climate variables and lagtime. Results: The study showed significant relationship between the number of dengue cases, rainfall, temperature, humidity and the incidence of dengue (p<0.05). Incidence of dengue in Denpasar city was affected by climate variability of 4-week period (lagtime 4) earlier and the number of dengue cases wasfrom two weeks earlier. Thus climate factors affected the incidence of dengue indirectly. Conclusion: The prediction model can be used as one of the considerations on the early warning of dengue disease in Denpasar city, while providing counseling or education efforts to the community about prevention of dengue and vector elimination
      PubDate: 2017-12-31
      Issue No: Vol. 8 (2017)
  • Biochemical characterization of insecticide resistance and exposure in
           Aedes aegypti population from Wonosobo, Central Java, Indonesia

    • Authors: Dyah Widiastuti
      Abstract: Resistance to insecticides mainly occurs due to changes in insect metabolic enzyme. Increased metabolism is often caused by qualitative or quantitative changes of esterase and glutathione S-transferase. Susceptibility test and biochemical assay to detect organophosphate and synthetic pyrethroid resistance were conducted on Aedes aegypti from Wonosobo (new highland Dengue endemic area). The test were performed on F1 generation of Ae.aegypti field caught mosquitoes which aimed to determine the resistance mechanisms regarding two detoxifying enzymes i.e. esterase and glutathione S-transferase. Susceptibility test showed 23.4 and 46.7% mortalities after exposure to 0.8% malathion and 0.05% cypermethrin. The biochemical assay result suggested that esterase, monooxygenase and glutathione s-transferase activity tend to increase in Ae.aegypti in Wonosobo. Interview and questionaires conclude that synthethic pyrethroid was the only insecticide type used in vector control program by Wonosobo Health Office and was the most frequent insecticide type to be used in household by Wonosobo society to control Ae.aegypti population. Knowledge of localized resistance and underlying mechanisms helps in making rational decisions in selection of appropriate and effective insecticides in the event of a dengue outbreak.
      PubDate: 2017-12-31
      Issue No: Vol. 8 (2017)
  • Strengthening Laboratory Capacity for Early Warning Alert and Response
           System (EWARS) in Indonesia

    • Authors: Nirmal Kandel, Ratna Budi Hapsari, Dyah Armi Riana, Vivi Setiawaty, Wita Larasati, Endang Wulandari, Edy Purwanto
      Abstract: Background: Establishment of Early Warning Alert and Response System (EWARS) in Indonesia was initiated since 2009 in few selected provinces and government was planning to roll out in other provinces. Before initiating this roll out the assessment of performance of EWARS in 2012 was conducted. The aim of the  assessment is to strengthen laboratory for supporting to EWARS for alerts testing. Methods: Laboratory capacity mapping tool and laboratory algorithm for twenty two priority diseases of EWARS were developed. This mapping tool was used in nine provinces to look at the existing capacities. This mapping tool and algorithm is used to pilot the laboratory support function for EWARS in four districts of West Kalimantan. Results: Almost all alerts have no laboratory confirmation except for few disease like measles, poliomyelitis and malaria. Capacity mapping of five of the nine provinces have available capacity for testing for bacteriology, virology, parasitology, mycology, hematology, immunology and environmental/chemicals in public or private sectors. A piloting of laboratory support for EWARS demonstrated the application of algorithm, development of logistic planning and allocation of resources for laboratory testing for alerts of EWARS. Conclusion: Capacity mapping opens horizon on strengthening support function of the system, which are readily available at local levels and provides opportunities for coordination and collaboration. EWARS is an opportunity to strengthen sustainable and sensitive surveillance system, which in turn assist on overall of health development through health systems strengthening. Keywords : Early Warning System, Laboratory Capacity, mapping
      PubDate: 2017-12-31
      Issue No: Vol. 8 (2017)
  • The Characteristics of Mothers Who Experienced Perinatal Depression and
           the Characteristics of Their Babies in Three Community Health Center in
           Jakarta and Bogor.

    • Authors: Sri Idaiani
      PubDate: 2017-12-31
      Issue No: Vol. 8 (2017)
  • Improving the use of Curcuma aeruginosa Roxb. as anthelmintic for children
           in Indonesia

    • Authors: Selma Arsit Selto Siahaan
      Abstract: This article based on the study results aims to improve the knowledge, attitude and practice (KAP) of mothers about self-medication of worm infections using Curcuma aeruginosa Roxb., a natural plant easily  found in Indonesia.  It consists of assessment and intervention to mothers(189) and children(204). Samples were collected from two Village Integrated Health Post in Cibungbulang village Bogor Municipality West Java Province. The KAP of mothers increased after they got health education & information and 95% of children who joined Curcuma treatment confirmed curing from worm infections, most of them 3 years old. Deworming program for children with Curcuma should be carried out routinely at least twice a year.Key words: deworming, Curcuma aeruginosa Roxb., anthelmintic, mothers and children.
      PubDate: 2017-12-31
      Issue No: Vol. 8 (2017)
  • The impact of vitamin A fortified vegetable oil on vitamin A status of
           children under five years of age: A cohort-like study

    • Authors: Sudikno Sudikno, Sandjaja Sandjaja, Idrus Jus'at
      Abstract: Introduction: Sub-clinical vitamin A deficiency (VAD) and anemia remain major nutritional problem in Indonesia. Although the government has implemented distribution of vitamin A capsules (VAC), there are one third of children missed VAC distribtion. The prevalence of anemia is also still high. Objectives: Part of this study was conducted to measure the effectiveness of vitamin A fortification in unbranded cooking oil among 6-59-month-old children of poor families in 2 districts in Indonesia prior mandatory vitamin A fortification in cooking oil. Materials and Methods: Total number of samples were 126 children. Venous blood was drawn by trained phlebotomist. Serum retinol and hemoglobin were measured by HPLC and hemoque respectively at baseline just before cooking oil fortification and 12 months after at endline. Trained enumerators collected data of morbidity and measurement of vitamin A content in cooking oil from the households, food stalls and markets was done periodically during the study. There was not any intervention from the study team on distribution and purchase of fortified cooking oil by the families, because cooking oil was distributed and sold through exsisting market mechanism. Enumerators collected socio-demographic variables. They also collected 24-hr dietary recall and food frequency questionnaires to measure nutrient intakes at base- and endline. Results: Serum retinol significantly increased by 5.07, 6.82, 6.01 µg/dL in 6-11, 12-23, and 24-59 month-old children respectively. Hemoglobin increased by 0.13 (p>0.05) , 0.56 (p<0.05), 0.81 g/dL (p<0.05) in 6-11, 12-35, 36-59 month-old children respectively. Conclusions: Vitamin A fortification in cooking oil significantly improved serum retinol in underfive children in all age groups and hemoglobin only in older age groups
      PubDate: 2017-12-31
      Issue No: Vol. 8 (2017)
  • Modification of Calgary-Cambridge Observation Guide, A More Simplified and
           Practical Guide for Daily Consultation Practice

    • Authors: Herqutanto Herqutanto
      Abstract: Background: The Calgary-Cambridge Observation Guides (CCOG) is a guide that is widely used to assess Doctor-Patient Communication. The guide consists of 56 points divided into 6 categories that describe a routine consultation process, plus 15 optional points in giving explanation and planning. Due to its quite numerous points, it is quite impractical to use the guide in daily consultation practice. Therefore, a more simplified and more practical version would be favourable. Method: Six experts from different background evaluated and analysed the 56 points of CCOG based on the level of importance in daily practice. Two rounds of Delphy were used. The result of the two rounds was then recirculated to obtain confirmation of the final modified version of CCOG. Result and Discussion: A final modified version of CCOG consisting of 35 points was formed. The first step of a consultation process, Initiating the session consists of 5 points (originally 7 points). Gathering information step consists of 5 points (originally 11 points), Providing structure of 3 points (originally 4 points), Building relationship of 7 points (originally 10 points), Explanation and Planning of 11 points (originally 20 points), and Closing the Session consisting of 4 points. The modified CCOG version is still comprehensive, yet more practical for daily practice. Conclusion: Modified version of CCOG can be used as a simple, practical guide to assess Doctor Patient Communication in daily consultation practice. Keywords: The Calgary-Cambridge Observation Guide, Doctor Patient Communication, Modification
      PubDate: 2017-12-31
      Issue No: Vol. 8 (2017)
  • ¬¨Predicting The Sick Building Syndrome (SBS) Incidence Among Pharmacist
           Assistant in Banjarmasin South Kalimantan

    • Authors: Muhamad Ratodi, Tien Zubaidah, Lenie Marlinae
      Abstract: Latar belakang: Banjarmasin memiliki jumlah apotek terbanyak di wilayah Kalimantan Selatan. Salahsatu jenis pekerjaan yang ada di apotek adalah asisten apoteker. Dengan tipikal pekerjaan asisten apoteker yang indoor dan memiliki resiko yang tinggi terhadap paparan zat kimia, asisten apoteker cenderung mengalami kejadian Sick Building Syndrom. Penelitian ini bertujuan untuk mengidentifikasi  faktor determinan yang paling berpengaruh terhadap kejadian SBS pada asisten apoteker yang bekerja di apotek wilayah kerja Kota Banjarmasin Metode: Penelitian ini merupakan penelitian crosssectional dengan metode observasional analitik. Lokasi penelitian dilakukan pada 13 lokasi apotek yang berada di wilayah kerja Kota Banjarmasin dengan 73 orang asisten apoteker yang memenuhi kriteria inklusi sebagai respondennya. Variabel bebas dari penelitian ini adalah variabel karakteristik responden dengan sub variabel jenis kelamin, usia, kondisi psikososial dan masa kerja, variabel aspek perilaku dengan sub variabel pengetahuan, sikap dan praktik asisten apoteker terhadap SBS, serta variabel faktor lingkungan pekerja meliputi kepadatan ruang, suhu, kelembaban, kecepatan aliran udara serta pencahayaan ruangan, sedangkan kejadian SBS menjadi variabel terikat. Hasil : Sebanyak 29 responden (39.7%) responden mengalami kejadian SBS dengan sebesar 68.3% kejadian SBS dipengaruhi oleh faktor psikososial responden (OR 26,479), masa kerja (OR 9,882), tindakan yang memicu SBS oleh responden (OR 13,859) serta kondisi pencahayaan yang tidak sesuai standar (OR 8,912). Kesimpulan : Untuk mengurangi angka kejadian SBS pada asisten apoteker maka diharapkan para pihak pengelola apotek di wilayah kerja kota Banjarmasin dapat melakukan pendekatan personal untuk mengurangi resiko paparan stress, melakukan pengaturan jam kerja untuk mengurangi keterpaparan dalam jangka waktu yang berlebihan, menerapkan pengaturan pencahayaan sesuai standar serta menyediakan waktu dan tempat terbuka bagi pekerjanya untuk beristirahat.
      PubDate: 2017-12-31
      Issue No: Vol. 8 (2017)

    • Authors: Meilinah Hidayat, Sijani Prahastuti, Ellya Rosa Delima, Andreanus Andaja Soemardji, Liasisca Setiawati
      Abstract: As an antiobesity, combination extract of Detam-1 soybean and Jati Belanda leaves must be safe and free from toxic agent, so the test of acute toxicity effect is needed. The aim of this study is to determine LD50 value, behavior, organ weight (OW), and organ index (OI) on mice after administrating Extract Ethanol Detam-1 soybean (EEDS), Jati Belanda ethanol extract (EEJB) and their combination. The methods which is used is  true experimental research with completely randomized design according to BPOM 2014.  Twenty female Swiss Webster mice  were divided into four treatment groups. Group 1 (negative control), 2 (EEDS  2.000 mg/kgBW), 3 (EEJB 2.000 mg/kgBW), and 4 (combination of EEDS and EEJB 1:2). Data  were analyzed using independent t test, α = 0.05. The result is there were no mortality and toxicity in all groups,  no significant difference of OW and OI of eight major organs within the groups statistically (p> 0.05), except lungs, liver and spleen in group 2. The conclusion is LD50 value of EEDS, EEJB and their combination were above 2.000 mg/kgBW, no toxic effect on the behavior of mice; OW and OI on mice after administrating EEDS, EEJB and their combination.
      PubDate: 2017-12-31
      Issue No: Vol. 8 (2017)
  • Front Metter HSJI Volume 8 no.2 2017

    • Authors: Front Metter HSJI Volume 8 no 2 tahun 2017
      Issue No: Vol. 8
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
Tel: +00 44 (0)131 4513762

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