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Publisher: Cambridge University Press   (Total: 371 journals)

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Showing 1 - 200 of 371 Journals sorted alphabetically
Acta Neuropsychiatrica     Hybrid Journal   (Followers: 4, SJR: 0.733, CiteScore: 2)
Acta Numerica     Hybrid Journal   (Followers: 3, SJR: 6.709, CiteScore: 10)
Advances in Animal Biosciences     Full-text available via subscription   (Followers: 9)
Advances in Applied Mathematics and Mechanics     Full-text available via subscription   (SJR: 0.441, CiteScore: 1)
Aeronautical J., The     Hybrid Journal   (Followers: 3)
Africa     Hybrid Journal   (Followers: 19, SJR: 0.582, CiteScore: 1)
African Studies Review     Full-text available via subscription   (Followers: 18, SJR: 0.437, CiteScore: 1)
Ageing & Society     Hybrid Journal   (Followers: 40, SJR: 0.756, CiteScore: 2)
Agricultural and Resource Economics Review     Open Access   (Followers: 5, SJR: 0.414, CiteScore: 1)
AI EDAM     Hybrid Journal   (SJR: 0.375, CiteScore: 1)
AJS Review     Full-text available via subscription   (Followers: 2, SJR: 0.128, CiteScore: 0)
American Political Science Review     Hybrid Journal   (Followers: 257, SJR: 5.587, CiteScore: 4)
Anatolian Studies     Full-text available via subscription   (Followers: 4, SJR: 0.528, CiteScore: 1)
Ancient Mesoamerica     Hybrid Journal   (Followers: 10, SJR: 0.478, CiteScore: 1)
Anglo-Saxon England     Hybrid Journal   (Followers: 35, SJR: 0.1, CiteScore: 0)
animal     Hybrid Journal   (Followers: 3, SJR: 0.842, CiteScore: 2)
Animal Health Research Reviews     Hybrid Journal   (Followers: 3, SJR: 0.69, CiteScore: 2)
Animal Science     Full-text available via subscription   (Followers: 9)
Annals of Actuarial Science     Full-text available via subscription   (Followers: 1)
Annual of the British School at Athens     Full-text available via subscription   (Followers: 15, SJR: 0.177, CiteScore: 0)
Annual Review of Applied Linguistics     Hybrid Journal   (Followers: 36, SJR: 3.223, CiteScore: 4)
Antarctic Science     Hybrid Journal   (Followers: 1, SJR: 0.643, CiteScore: 1)
Antichthon     Full-text available via subscription   (Followers: 1, SJR: 0.101, CiteScore: 0)
Antiquaries J., The     Full-text available via subscription   (Followers: 8, SJR: 0.106, CiteScore: 0)
Antiquity     Hybrid Journal   (Followers: 27)
ANZIAM J.     Open Access   (Followers: 1, SJR: 0.216, CiteScore: 0)
Applied Psycholinguistics     Hybrid Journal   (Followers: 22, SJR: 0.945, CiteScore: 2)
APSIPA Transactions on Signal and Information Processing     Open Access   (Followers: 8, SJR: 0.404, CiteScore: 2)
Arabic Sciences and Philosophy     Hybrid Journal   (Followers: 9, SJR: 0.101, CiteScore: 0)
Arbor Clinical Nutrition Updates     Full-text available via subscription   (Followers: 3)
Archaeological Dialogues     Hybrid Journal   (Followers: 36, SJR: 0.898, CiteScore: 1)
Archaeological Reports     Full-text available via subscription   (Followers: 5, SJR: 0.128, CiteScore: 0)
arq: Architectural Research Quarterly     Hybrid Journal   (Followers: 8, SJR: 0.123, CiteScore: 0)
Asian J. of Comparative Law     Hybrid Journal   (Followers: 9, SJR: 0.129, CiteScore: 0)
Asian J. of Intl. Law     Hybrid Journal   (Followers: 14, SJR: 0.135, CiteScore: 0)
Asian J. of Law and Society     Hybrid Journal   (Followers: 7, SJR: 0.195, CiteScore: 0)
Astin Bulletin     Full-text available via subscription   (Followers: 1, SJR: 0.878, CiteScore: 1)
Australasian J. of Organisational Psychology     Hybrid Journal   (Followers: 9, SJR: 0.154, CiteScore: 1)
Australasian J. of Special Education     Full-text available via subscription   (Followers: 8, SJR: 0.187, CiteScore: 0)
Australian J. of Environmental Education     Full-text available via subscription   (Followers: 7, SJR: 0.403, CiteScore: 1)
Australian J. of Indigenous Education, The     Full-text available via subscription   (Followers: 10, SJR: 0.26, CiteScore: 1)
Australian J. of Rehabilitation Counseling     Full-text available via subscription   (Followers: 4, SJR: 0.144, CiteScore: 0)
Austrian History Yearbook     Full-text available via subscription   (Followers: 9, SJR: 0.161, CiteScore: 0)
Behavioral and Brain Sciences     Hybrid Journal   (Followers: 34, SJR: 0.595, CiteScore: 1)
Behaviour Change     Full-text available via subscription   (Followers: 13, SJR: 0.508, CiteScore: 1)
Behavioural and Cognitive Psychotherapy     Hybrid Journal   (Followers: 139, SJR: 0.976, CiteScore: 2)
Bilingualism: Language and Cognition     Hybrid Journal   (Followers: 37, SJR: 1.446, CiteScore: 2)
Biofilms     Full-text available via subscription   (Followers: 1)
Bird Conservation Intl.     Hybrid Journal   (Followers: 24, SJR: 0.581, CiteScore: 1)
BJPsych Advances     Full-text available via subscription   (Followers: 54, SJR: 0.275, CiteScore: 0)
BJPsych Intl.     Open Access   (Followers: 2)
BJPsych Open     Open Access  
Brain Impairment     Full-text available via subscription   (Followers: 2, SJR: 0.321, CiteScore: 1)
Breast Cancer Online     Full-text available via subscription   (Followers: 4)
Britannia     Full-text available via subscription   (Followers: 7, SJR: 0.111, CiteScore: 0)
British Actuarial J.     Full-text available via subscription  
British Catholic History     Hybrid Journal   (Followers: 1, SJR: 0.133, CiteScore: 1)
British J. for the History of Science     Hybrid Journal   (Followers: 22, SJR: 0.235, CiteScore: 0)
British J. of Anaesthetic and Recovery Nursing     Full-text available via subscription   (Followers: 8)
British J. of Music Education     Hybrid Journal   (Followers: 24, SJR: 0.564, CiteScore: 1)
British J. Of Nutrition     Hybrid Journal   (Followers: 74, SJR: 1.612, CiteScore: 4)
British J. of Political Science     Hybrid Journal   (Followers: 171, SJR: 4.661, CiteScore: 4)
British J. of Psychiatry     Hybrid Journal   (Followers: 191, SJR: 2.844, CiteScore: 3)
Bulletin of Entomological Research     Hybrid Journal   (Followers: 11, SJR: 0.805, CiteScore: 2)
Bulletin of Symbolic Logic     Full-text available via subscription   (Followers: 2, SJR: 0.555, CiteScore: 1)
Bulletin of the Australian Mathematical Society     Full-text available via subscription   (Followers: 1, SJR: 0.44, CiteScore: 0)
Bulletin of the School of Oriental and African Studies     Hybrid Journal   (Followers: 19, SJR: 0.146, CiteScore: 0)
Business and Human Rights J.     Full-text available via subscription   (Followers: 3, SJR: 0.536, CiteScore: 1)
Business Ethics Quarterly     Full-text available via subscription   (Followers: 13, SJR: 1.098, CiteScore: 2)
Business History Review     Full-text available via subscription   (Followers: 15, SJR: 0.347, CiteScore: 1)
Cambridge Archaeological J.     Hybrid Journal   (Followers: 129, SJR: 1.121, CiteScore: 1)
Cambridge Classical J.     Full-text available via subscription   (Followers: 18, SJR: 0.101, CiteScore: 0)
Cambridge J. of Postcolonial Literary Inquiry     Hybrid Journal   (Followers: 7)
Cambridge Law J.     Hybrid Journal   (Followers: 166, SJR: 0.213, CiteScore: 0)
Cambridge Opera J.     Hybrid Journal   (Followers: 5, SJR: 0.14, CiteScore: 0)
Cambridge Quarterly of Healthcare Ethics     Hybrid Journal   (Followers: 11, SJR: 0.299, CiteScore: 1)
Camden Fifth Series     Full-text available via subscription   (Followers: 3)
Canadian Entomologist     Hybrid Journal   (Followers: 5, SJR: 0.482, CiteScore: 1)
Canadian J. of Emergency Medicine     Hybrid Journal   (Followers: 12, SJR: 0.624, CiteScore: 1)
Canadian J. of Law & Jurisprudence     Full-text available via subscription   (Followers: 10, SJR: 0.237, CiteScore: 0)
Canadian J. of Law and Society     Hybrid Journal   (Followers: 20, SJR: 0.259, CiteScore: 1)
Canadian J. of Neurological Sciences     Full-text available via subscription   (SJR: 0.549, CiteScore: 1)
Canadian J. of Political Science/Revue canadienne de science politique     Full-text available via subscription   (Followers: 23, SJR: 0.385, CiteScore: 1)
Canadian J. on Aging     Hybrid Journal   (Followers: 10, SJR: 0.426, CiteScore: 1)
Canadian Yearbook of Intl. Law / Annuaire canadien de droit international     Full-text available via subscription   (Followers: 2)
Cardiology in the Young     Hybrid Journal   (Followers: 32, SJR: 0.372, CiteScore: 1)
Central European History     Full-text available via subscription   (Followers: 30, SJR: 0.159, CiteScore: 0)
Children Australia     Partially Free   (Followers: 2, SJR: 0.255, CiteScore: 0)
China Quarterly     Hybrid Journal   (Followers: 51, SJR: 2.289, CiteScore: 3)
Chinese J. of Agricultural Biotechnology     Full-text available via subscription   (Followers: 4)
Church History: Studies in Christianity and Culture     Full-text available via subscription   (Followers: 72, SJR: 0.106, CiteScore: 0)
Classical Quarterly     Full-text available via subscription   (Followers: 29, SJR: 0.204, CiteScore: 0)
Classical Review     Full-text available via subscription   (Followers: 25)
CNS Spectrums     Hybrid Journal   (Followers: 3, SJR: 1.391, CiteScore: 3)
Cognitive Behaviour Therapist     Hybrid Journal   (Followers: 13)
Combinatorics, Probability and Computing     Hybrid Journal   (Followers: 4, SJR: 0.839, CiteScore: 1)
Communications in Computational Physics     Full-text available via subscription   (Followers: 2, SJR: 1.048, CiteScore: 2)
Comparative Studies in Society and History     Full-text available via subscription   (Followers: 47, SJR: 0.585, CiteScore: 1)
Compositio Mathematica     Full-text available via subscription   (Followers: 1, SJR: 3.139, CiteScore: 1)
Contemporary European History     Hybrid Journal   (Followers: 25, SJR: 0.263, CiteScore: 1)
Continuity and Change     Hybrid Journal   (Followers: 12, SJR: 0.107, CiteScore: 0)
Dance Research J.     Full-text available via subscription   (Followers: 9, SJR: 0.211, CiteScore: 0)
Development and Psychopathology     Hybrid Journal   (Followers: 9, SJR: 2.068, CiteScore: 4)
Dialogue Canadian Philosophical Review/Revue canadienne de philosophie     Full-text available via subscription   (Followers: 3, SJR: 0.156, CiteScore: 0)
Diamond Light Source Proceedings     Full-text available via subscription  
Disaster Medicine and Public Health Preparedness     Hybrid Journal   (Followers: 13, SJR: 0.471, CiteScore: 1)
Du Bois Review: Social Science Research on Race     Full-text available via subscription   (Followers: 9, SJR: 0.561, CiteScore: 1)
Early China     Hybrid Journal   (Followers: 3)
Early Music History     Hybrid Journal   (Followers: 8, SJR: 0.101, CiteScore: 0)
Earth and Environmental Science Transactions of the Royal Society of Edinburgh     Hybrid Journal   (Followers: 5)
East Asian J. on Applied Mathematics     Full-text available via subscription   (SJR: 0.418, CiteScore: 1)
Ecclesiastical Law J.     Full-text available via subscription   (Followers: 6, SJR: 0.114, CiteScore: 0)
Econometric Theory     Hybrid Journal   (Followers: 16, SJR: 2.915, CiteScore: 1)
Economics and Philosophy     Hybrid Journal   (Followers: 14, SJR: 0.622, CiteScore: 1)
Edinburgh J. of Botany     Hybrid Journal   (SJR: 0.283, CiteScore: 1)
Eighteenth-Century Music     Hybrid Journal   (Followers: 11, SJR: 0.113, CiteScore: 0)
English Language and Linguistics     Hybrid Journal   (Followers: 24, SJR: 0.52, CiteScore: 1)
English Profile J.     Hybrid Journal   (Followers: 2)
English Today     Hybrid Journal   (Followers: 8, SJR: 0.279, CiteScore: 0)
Enterprise & Society : The Intl. J. of Business History     Hybrid Journal   (Followers: 17, SJR: 0.245, CiteScore: 1)
Environment and Development Economics     Hybrid Journal   (Followers: 33, SJR: 0.617, CiteScore: 1)
Environmental Conservation     Hybrid Journal   (Followers: 59, SJR: 1.028, CiteScore: 2)
Environmental Practice     Full-text available via subscription   (Followers: 3, SJR: 0.145, CiteScore: 0)
Epidemiology & Infection     Hybrid Journal   (Followers: 15, SJR: 1.128, CiteScore: 2)
Epidemiology and Psychiatric Sciences     Hybrid Journal   (Followers: 3, SJR: 1.494, CiteScore: 2)
Episteme     Hybrid Journal   (Followers: 12, SJR: 0.756, CiteScore: 1)
Equine and Comparative Exercise Physiology     Full-text available via subscription   (Followers: 6)
Ergodic Theory and Dynamical Systems     Hybrid Journal   (Followers: 2, SJR: 1.193, CiteScore: 1)
Ethics & Intl. Affairs     Full-text available via subscription   (Followers: 13, SJR: 0.557, CiteScore: 1)
European Constitutional Law Review (EuConst)     Full-text available via subscription   (Followers: 32, SJR: 1.009, CiteScore: 1)
European J. of Applied Mathematics     Hybrid Journal   (SJR: 0.52, CiteScore: 1)
European J. of Sociology     Hybrid Journal   (Followers: 30, SJR: 0.643, CiteScore: 1)
European Political Science Review     Hybrid Journal   (Followers: 21, SJR: 1.816, CiteScore: 2)
European Review     Hybrid Journal   (Followers: 17, SJR: 0.131, CiteScore: 0)
Experimental Agriculture     Hybrid Journal   (Followers: 13, SJR: 0.542, CiteScore: 1)
Expert Reviews in Molecular Medicine     Hybrid Journal   (Followers: 1, SJR: 1.647, CiteScore: 4)
Fetal and Maternal Medicine Review     Hybrid Journal   (Followers: 4)
Financial History Review     Full-text available via subscription   (Followers: 14, SJR: 0.238, CiteScore: 1)
Foreign Policy Bulletin     Hybrid Journal   (Followers: 6)
Forum of Mathematics, Pi     Open Access   (Followers: 1)
Forum of Mathematics, Sigma     Open Access   (Followers: 1)
Genetics Research     Hybrid Journal   (Followers: 4, SJR: 0.483, CiteScore: 1)
Geological Magazine     Hybrid Journal   (Followers: 16, SJR: 0.966, CiteScore: 2)
Glasgow Mathematical J.     Full-text available via subscription   (Followers: 1, SJR: 0.604, CiteScore: 0)
Global Constitutionalism     Hybrid Journal   (Followers: 16)
Global Mental Health     Open Access   (Followers: 7)
Government and Opposition     Full-text available via subscription   (Followers: 20, SJR: 0.965, CiteScore: 2)
Greece & Rome     Partially Free   (Followers: 21, SJR: 0.113, CiteScore: 0)
Hague J. on the Rule of Law     Full-text available via subscription   (Followers: 13, SJR: 0.271, CiteScore: 1)
Harvard Theological Review     Full-text available via subscription   (Followers: 66, SJR: 0.165, CiteScore: 0)
Health Economics, Policy and Law     Hybrid Journal   (Followers: 25, SJR: 0.745, CiteScore: 1)
Hegel Bulletin     Full-text available via subscription   (Followers: 1)
High Power Laser Science and Engineering     Open Access   (Followers: 3, SJR: 0.901, CiteScore: 3)
Historical J.     Hybrid Journal   (Followers: 34, SJR: 0.247, CiteScore: 1)
History in Africa     Full-text available via subscription   (Followers: 8)
Horizons     Partially Free   (Followers: 1, SJR: 0.129, CiteScore: 0)
Industrial and Organizational Psychology     Hybrid Journal   (Followers: 20, SJR: 0.916, CiteScore: 1)
Infection Control and Hospital Epidemiology     Full-text available via subscription   (Followers: 36, SJR: 1.97, CiteScore: 3)
Intl. & Comparative Law Quarterly     Full-text available via subscription   (Followers: 206, SJR: 0.369, CiteScore: 1)
Intl. J. of Asian Studies     Hybrid Journal   (Followers: 12, SJR: 0.143, CiteScore: 0)
Intl. J. of Astrobiology     Hybrid Journal   (Followers: 2, SJR: 0.548, CiteScore: 1)
Intl. J. of Cultural Property     Full-text available via subscription   (Followers: 13, SJR: 0.253, CiteScore: 1)
Intl. J. of Disability Management Research     Full-text available via subscription   (Followers: 8, SJR: 0.105, CiteScore: 0)
Intl. J. of Law in Context     Hybrid Journal   (Followers: 16, SJR: 0.275, CiteScore: 1)
Intl. J. of Microwave and Wireless Technologies     Hybrid Journal   (Followers: 8, SJR: 0.184, CiteScore: 1)
Intl. J. of Middle East Studies     Hybrid Journal   (Followers: 66, SJR: 0.434, CiteScore: 0)
Intl. J. of Technology Assessment in Health Care     Hybrid Journal   (Followers: 13, SJR: 0.714, CiteScore: 1)
Intl. J. of Tropical Insect Science     Hybrid Journal   (Followers: 1, SJR: 0.334, CiteScore: 1)
Intl. Labor and Working-Class History     Full-text available via subscription   (Followers: 11, SJR: 0.182, CiteScore: 0)
Intl. Organization     Full-text available via subscription   (Followers: 90, SJR: 8.527, CiteScore: 5)
Intl. Psychogeriatrics     Hybrid Journal   (Followers: 12, SJR: 1.048, CiteScore: 2)
Intl. Review of Social History     Full-text available via subscription   (Followers: 25, SJR: 0.315, CiteScore: 1)
Intl. Review of the Red Cross     Full-text available via subscription   (Followers: 10, SJR: 0.214, CiteScore: 0)
Intl. Theory: A J. of Intl. Politics, Law and Philosophy     Hybrid Journal   (Followers: 18, SJR: 2.293, CiteScore: 2)
Iraq     Full-text available via subscription   (Followers: 2)
Irish Historical Studies     Hybrid Journal   (Followers: 5, SJR: 0.103, CiteScore: 0)
Irish J. of Psychological Medicine     Hybrid Journal   (Followers: 2, SJR: 0.221, CiteScore: 0)
Israel Law Review     Hybrid Journal   (Followers: 2, SJR: 0.165, CiteScore: 0)
Itinerario     Full-text available via subscription   (Followers: 9, SJR: 0.158, CiteScore: 0)
J. of African History     Hybrid Journal   (Followers: 21, SJR: 0.348, CiteScore: 1)
J. of African Law     Full-text available via subscription   (Followers: 3, SJR: 0.113, CiteScore: 0)
J. of Agricultural and Applied Economics     Open Access   (Followers: 1, SJR: 0.263, CiteScore: 1)
J. of Agricultural Science     Full-text available via subscription   (Followers: 8, SJR: 0.563, CiteScore: 1)
J. of American Studies     Hybrid Journal   (Followers: 20, SJR: 0.164, CiteScore: 0)
J. of Anglican Studies     Hybrid Journal   (Followers: 6, SJR: 0.101, CiteScore: 0)
J. of Applied Animal Nutrition     Hybrid Journal   (Followers: 3)
J. of Asian Studies     Full-text available via subscription   (Followers: 37, SJR: 0.591, CiteScore: 1)
J. of Benefit-Cost Analysis     Hybrid Journal   (Followers: 2)
J. of Biosocial Science     Hybrid Journal   (Followers: 3, SJR: 0.48, CiteScore: 1)
J. of British Studies     Full-text available via subscription   (Followers: 31, SJR: 0.246, CiteScore: 0)
J. of Child Language     Hybrid Journal   (Followers: 20, SJR: 1.035, CiteScore: 2)
J. of Classics Teaching     Open Access  
J. of Dairy Research     Full-text available via subscription   (Followers: 7, SJR: 0.573, CiteScore: 1)
J. of Demographic Economics     Hybrid Journal   (Followers: 3, SJR: 1.227, CiteScore: 1)
J. of Developmental Origins of Health and Disease     Hybrid Journal   (Followers: 2, SJR: 0.843, CiteScore: 2)
J. of Diagnostic Radiography and Imaging     Hybrid Journal   (Followers: 4)
J. of East Asian Studies     Full-text available via subscription   (Followers: 12, SJR: 0.59, CiteScore: 1)
J. of Ecclesiastical History     Hybrid Journal   (Followers: 19, SJR: 0.138, CiteScore: 0)
J. of Economic History     Full-text available via subscription   (Followers: 44, SJR: 1.82, CiteScore: 2)
J. of Experimental Political Science     Full-text available via subscription   (Followers: 5, SJR: 2.526, CiteScore: 2)

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Journal Cover
Chinese Journal of Agricultural Biotechnology
Number of Followers: 4  
 
  Full-text available via subscription Subscription journal
ISSN (Print) 1479-2362
Published by Cambridge University Press Homepage  [371 journals]
  • CJA volume 6 issue 3 Cover and Front matter
    • PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990556
      Issue No: Vol. 6, No. 3 (2009)
       
  • CJA volume 6 issue 3 Cover and Back matter
    • PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990568
      Issue No: Vol. 6, No. 3 (2009)
       
  • Collection and preservation of porcine polar bodies
    • Authors: Wang Gong-Jin; Zhou Xiao-Long, Tan Xiao-Dong, Yu Jian-Ning, Xu Xiao-Bo, Fan Bi-Qin
      Pages: 183 - 189
      Abstract: Mature porcine oocytes containing first polar bodies (Pb I) were obtained by in vitro culture of follicle oocytes from ovaries obtained from a local abattoir, and zygotes with second polar bodies (Pb II) were grown after in vitro fertilization of the mature oocytes. Extrusion, biological activity and morphology of Pb I and Pb II were statistically analysed. Polar bodies were isolated and collected from oocytes by enzyme digestion or micromanipulation. Their vigour under different preservation conditions was analysed and evaluated using a Trypan blue staining method. The results showed that 66.7% of the oocytes extruded Pb I after 40 h of in vitro mature culture of oocytes, and 49.7% of the zygotes extruded Pb II 20 h after in vitro fertilization. The efficiency of isolation of Pb II by micromanipulation significantly exceeded that by enzyme digestion, the Pb I and Pb II isolated by micromanipulation presenting with good vigour and normal morphology (95.3% versus 58.9%). The survival rates of Pb I and Pb II were 63.3% and 93.1% for 4 h at 39°C, 85.0% and 72.9% for 40 h at 4°C, and over 95.0% and 84.6% for less than 7 days at −20°C. In comparison with the above preservation conditions for Pb I and Pb II, the results for cryopreservation were best, with rates of survival as high as 89.1% for Pb I and 87.9% for Pb II for preservation periods of over a month, and rates of normal morphology of 97.8% and 95.7%, respectively. The Pb I and Pb II could be isolated and preserved effectively, for use in further research on the recombination of oocytes and zygotes.
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990519
      Issue No: Vol. 6, No. 3 (2009)
       
  • VP1+and+3ABC+genes+from+yellow+cattle+isolates+persistently+infected+by+Foot-and-mouth+disease+virus&rft.title=Chinese+Journal+of+Agricultural+Biotechnology&rft.issn=1479-2362&rft.date=2009&rft.volume=6&rft.spage=191&rft.epage=197&rft.aulast=Xiao-yan&rft.aufirst=Shen&rft.au=Shen+Xiao-yan&rft.au=Cong+Guo-zheng,+Chang+Hui-yun,+Liu+Xiang-tao,+Xie+Qing-ge&rft_id=info:doi/10.1017/S1479236209990507">Variation analysis of the VP1 and 3ABC genes from yellow cattle isolates
           persistently infected by Foot-and-mouth disease virus
    • Authors: Shen Xiao-yan; Cong Guo-zheng, Chang Hui-yun, Liu Xiang-tao, Xie Qing-ge
      Pages: 191 - 197
      Abstract: The potential relationship between the establishment of Foot-and-mouth disease virus (FMDV) persistent infection and gene variation was identified by investigating the variation of VP1 and 3ABC genes from yellow cattle persistent infection isolates. Five yellow cattle were inoculated on their tongue with 1.0×104 ID50/ml of FMDV O/Akesu/58 strain. After displaying clinical or subclinical signs, they probably became asymptomatic carriers. Oesophageal–pharyngeal fluids were collected monthly from the carriers with a probang and inoculated into a baby hamster kidney cell line (BHK-21); 12 FMDV isolates were obtained. The VP1 and 3ABC genes of the 12 isolates were then amplified by reverse-transcriptase polymerase chain reaction (RT-PCR). Cloning and sequencing revealed that the homology of the VP1 nucleotide and amino-acid sequence of all the isolates was above 98%, with no base deletion or insertion. When compared with the O/Akesu/58 FMDV strain, the homology of the VP1 nucleotide sequence of the isolates was only 85%, and that of the deduced amino-acid sequence only 90%.There were several nucleotide mutations in the VP1 gene of the isolates, including 16 consistent nucleotide mutations, with only two of them leading to a change in amino acid (I56→T, A210→E). Moreover, it was found that four nucleotide points and three amino-acid points had transversions among all isolates. The 3ABC gene had only 13 nucleotide transversions and five amino-acid mutations. It was presumed that persistent FMDV infection might have little connection with variation in the VP1 and 3ABC genes, and was probably related to other structural protein gene and key factors.
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990507
      Issue No: Vol. 6, No. 3 (2009)
       
  • Barbodes+caldwelli+juvenile&rft.title=Chinese+Journal+of+Agricultural+Biotechnology&rft.issn=1479-2362&rft.date=2009&rft.volume=6&rft.spage=199&rft.epage=205&rft.aulast=Yao-Ping&rft.aufirst=Lv&rft.au=Lv+Yao-Ping&rft.au=Chen+Jian-Ming,+Ye+Jin-Yun,+Huang+Xu-Xiong,+Lao+Shen-Ying,+Shen+Bin-Qian,+Yao+Zi-Liang,+Guo+Jian-Lin,+Ye+Li-Ping&rft_id=info:doi/10.1017/S1479236209990222">The effects of diet protein levels on the growth, body composition and
           digestive enzyme activities of the Barbodes caldwelli juvenile
    • Authors: Lv Yao-Ping; Chen Jian-Ming, Ye Jin-Yun, Huang Xu-Xiong, Lao Shen-Ying, Shen Bin-Qian, Yao Zi-Liang, Guo Jian-Lin, Ye Li-Ping
      Pages: 199 - 205
      Abstract: Seven isoenergetic semi-purified test diets containing graded levels of protein ranging from 20 to 50% were formulated using fish meal and casein as the protein sources. Test diets were fed to triplicate groups of Barbodes caldwelli juveniles with initial body weights of 1.26±0.02 g for eight weeks. The results indicated no significant effect of dietary protein levels on survival rate, relative weight of the viscera and relative weight of the liver in the juvenile fish. The weight gain and specific growth rate of the fish were found to be greater as dietary protein levels increased from 20 to 35%, but were not affected significantly as dietary protein level increased from 35 to 50%. Feed efficiencies did not differ significantly when fish were fed diets with protein levels from 30 to 50%, but were significantly higher than those of fish fed diets with protein levels of 20 and 25%. The protein efficiency ratio (PER) was negatively correlated with diet protein level (x) (PER=3.006−0.03251x, R=0.9366). There was no significant effect of dietary protein levels on carcass moisture, crude protein and ash. However, carcass lipid levels (L) decreased with an increase in dietary protein level (x) (L=8.2169−0.0458x, R=0.8551). There was no significant variation in hepatopancreas protease activity among the tests. Intestine protease activity and hepatopancreas amylase activity were increased to some extent, and then decreased as dietary protein levels continued to rise. The intestine amylase activity (IAA) of the juveniles was negatively correlated with dietary protein level (x) (IAA=84.625−0.9147x, R=0.8463). It was estimated that the suitable protein level for the B. caldwelli juvenile is 34% (the broken-line model was used to regress the relationship of the weight gain of the juvenile and dietary protein level).
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990222
      Issue No: Vol. 6, No. 3 (2009)
       
  • Cloning of 3′-end full-length cDNA encoding for MT-I and MT-III in yak
           and analysis of their sequences
    • Authors: Liu Bin; Zhang Li-Ping, Wu Jian-Ping, Ma Bin-Yun, Gao Feng-Qin, Yan Lian
      Pages: 207 - 214
      Abstract: Domestic yak (Bos grunniens) metallothionein-I (MT-I) and metallothionein-III (MT-III) cDNA 3′-end full-length sequences (331 and 378 bp; GenBank accession MT-I no. AY758557, MT-III no. DQ492300) from the total RNA of liver and brain tissues were amplified and cloned by reverse transcription polymerase chain reaction (RT-PCR) and 3′-rapid amplification of cDNA ends (RACE) using the primers YMT-I SP and M13 primers M4, and YMT-III SP and M13 primers M4, respectively, which included the MT-I (183 bp) coding sequence, MT-III (207 bp) cDNA coding sequences, and also the tailing signal AATAAA and Poly(A) at the 3′-ends of the MT-I and MT-III, respectively. The analyses showed that the yak cDNA sequence coding for MT-I protein was composed of 61 amino acids, including 20 cysteines having a conserved tripeptide structure, for example C–X–C, C–C–X–C–C, C–X–X–C and so on. The yak cDNA sequence coding for MT-III protein was composed of 68 amino acids, including 19 cysteines having both MT-III-specific conserved tripeptides and the same conserved tripeptides as MT-I, including T, CPCP, GEGAEA and so on. They were all comparatively conservative in molecular evolution. These structures indicated that MT-III had the same physiological functions regarding heavy metals (detoxification and so on) as MT-I, and also different specific functions from MT-I, including inhibition of neuron growth.
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990441
      Issue No: Vol. 6, No. 3 (2009)
       
  • CRS-PCR polymorphisms of the GHR gene and its relationship with milk
           production traits in Chinese Holstein cows
    • Authors: Wang Li-Juan; Li Qiu-Ling, Wang Chang-Fa, Wang Hong-Mei, Li Jian-Bin, Gao Yun-Dong, Hou Ming-Hai, Zhong Ji-Feng
      Pages: 215 - 219
      Abstract: Single-nucleotide polymorphisms (SNPs) of exon 8 of the GHR gene were detected in Chinese Holstein cows by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The results showed that exon 8 of the GHR gene digested by TasI could be divided into two kinds of alleles and three kinds of genotypes. The frequencies of allele A and T were 0.6339 and 0.3661, respectively. The frequencies of genotypes AA, AT and TT were 0.459, 0.350 and 0.191, respectively. Sequencing showed one single nucleotide mutation T→A at 4962 bp of the gene in genotype TT when compared with genotype AA, and this mutation resulted in an amino acid change of phenylalanine (TTT)→tyrosine (TAT). The result of χ2 testing indicated that the genotypic frequency of the GHR gene digested by TasI did not fit with Hardy–Weinberg equilibrium in this population (P
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990283
      Issue No: Vol. 6, No. 3 (2009)
       
  • Triticum+aestivum)+A+and+B+genomes&rft.title=Chinese+Journal+of+Agricultural+Biotechnology&rft.issn=1479-2362&rft.date=2009&rft.volume=6&rft.spage=221&rft.epage=226&rft.aulast=Zi-Xian&rft.aufirst=Zeng&rft.au=Zeng+Zi-Xian&rft.au=Yang+Zu-Jun,+Liu+Cheng,+Hu+Li-Jun,+Ren+Zheng-Long&rft_id=info:doi/10.1017/S1479236209990295">Isolation, mapping and application of a repetitive DNA sequence in wheat
           (Triticum aestivum) A and B genomes
    • Authors: Zeng Zi-Xian; Yang Zu-Jun, Liu Cheng, Hu Li-Jun, Ren Zheng-Long
      Pages: 221 - 226
      Abstract: Simple sequence repeat (SSR) analysis was performed on five Secale species, four Triticum species and a Triticale line Fenzhi-1 using 102 pairs of microsatellite primers. A 387-bp specific DNA fragment FZ387 (GenBank accession no. EF179137) was obtained from the Triticale Fenzhi-1 with primer Xgwm614, without amplification in Secale. NCBI BLAST revealed that this FZ387 sequence had 94% and 95% similarity to part of the Gypsy Ty3-LTR retrotransposon Fatima in Triticum monoccocum (AY485644) and Triticum turgidum (AY494981), respectively. A pair of specific polymerase chain reaction (PCR) primers, FaF and FaR, was designed based on the conserved region of this FZ387 sequence. The amplification of primer pair Xgwm614F and FaR revealed that a specific 350-bp band (designation as A350) was obtained from the species containing A chromosomes. Furthermore, PCR on Langdon Chinese Spring substitution lines was performed, and the results found that this segment was located on both long and short arms of all A chromosomes. However, the amplification of primer pair FaF and Xgwm614R gave rise to a specific DNA band of about 350 bp (designated AB350) from materials containing A and/or B chromosomes. The wild species of wheat and the relatives were amplified using the two pairs of primers, and revealed that only A350 and AB350 were found in Chinese Spring (CS). Sequence comparison and variation of SSR primers binding regions of FZ387 indicated that significant diversity might exist in the internal sequence of this Fatima-like element among triticeae genomes. Meanwhile, both A350 and AB350 can be used as molecular markers for the detection of A and AB genomes.
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990295
      Issue No: Vol. 6, No. 3 (2009)
       
  • Brassica+oleracea+C+genome+using+Brassica+A+genomic+DNA+as+blocking+agent&rft.title=Chinese+Journal+of+Agricultural+Biotechnology&rft.issn=1479-2362&rft.date=2009&rft.volume=6&rft.spage=227&rft.epage=233&rft.aulast=Xiao-Dan&rft.aufirst=Chen&rft.au=Chen+Xiao-Dan&rft.au=Zhu+Li-Quan,+Wang+Yong,+Rong+Xiao-Ying,+Lu+Jun,+Wang+Xiao-Jia&rft_id=info:doi/10.1017/S1479236209990143">Karyotyping of Brassica oleracea C genome using Brassica A genomic DNA as
           blocking agent
    • Authors: Chen Xiao-Dan; Zhu Li-Quan, Wang Yong, Rong Xiao-Ying, Lu Jun, Wang Xiao-Jia
      Pages: 227 - 233
      Abstract: In exploring an effective and reliable karyotyping method in Brassica crop plants, Brassica oleracea was successfully karyotyped using genomic in situ hybridization (GISH). B. oleracea genomic DNA was labelled as probe using DIG-high prime mix kit, with B. rapa genomic DNA acting as blocking agent. Specific fluorescent signals were detected on each pair of homologous chromosomes, and nine pairs of chromosomes of B. oleracea were clearly identified according to the signal characteristics. A practical and accurate method for conducting karyotyping of small chromosomes has been demonstrated.
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990143
      Issue No: Vol. 6, No. 3 (2009)
       
  • Identification of polymorphism in the goat callipyge gene (CLPG) and its
           associations with production traits
    • Authors: Cao Gui-Ling; Li Biao, Tang Hui, Tang Pei-Rong, Wang Jian-Min, Jiang Yun-Liang
      Pages: 235 - 240
      Abstract: The Dorset ram of the callipyge phenotype presents with muscular hypertrophy in the buttocks, and its inheritance is polar overdominant. A partial DNA fragment of 250 bp was obtained from the goat (Capra hircas) callipyge gene (CLPG; GenBank accession no. EU753362), which shared 96.04% and 88.65% identity with the corresponding regions of ovine (Ovis aries) and porcine CLPG, respectively. A polymorphism in the DNA fragment was detected by polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP). Sequencing results indicated no A→C mutation corresponding to the ovine CLPG gene, although one A→C transversion was located 147 bp downstream from the CLPG site. The polymorphism, named SNP216 after its position (where SNP indicates single-nucleotide polymorphism), was investigated in Boer (n=63), Laiwu Black (n=70), Lubei White×Boer Hybrid (n=40), Lubei White (n=29) and Inner Mongolia Alashan White cashmere (n=115) goat populations. The results indicated that allele A was dominant in four of the goat populations, the Inner Mongolia Alashan White cashmere goats being the exception. The first four populations were in a state of Hardy–Weinberg equilibrium (P>0.05). In Inner Mongolia Alashan White cashmere goats, least-square means of birth weight, production of cashmere and body weight gain from birth to weaning did not differ significantly between the AA and AC phenotypes (P>0.5).
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990544
      Issue No: Vol. 6, No. 3 (2009)
       
  • Brachymystax+lenok+populations+using+AFLP+markers&rft.title=Chinese+Journal+of+Agricultural+Biotechnology&rft.issn=1479-2362&rft.date=2009&rft.volume=6&rft.spage=241&rft.epage=247&rft.aulast=Di&rft.aufirst=Wang&rft.au=Wang+Di&rft.au=Li+Shao-Wu,+Xu+Ge-Feng,+Liu+Yang,+Mou+Zhen-Bo,+Lu+Tong-Yan&rft_id=info:doi/10.1017/S1479236209990106">Assessing genetic diversity in three wild Brachymystax lenok populations
           using AFLP markers
    • Authors: Wang Di; Li Shao-Wu, Xu Ge-Feng, Liu Yang, Mou Zhen-Bo, Lu Tong-Yan
      Pages: 241 - 247
      Abstract: The genetic diversities of 72 individuals from three wild Lenok populations of Mudanjiang River (MD), Yalujiang River (YL) and Wusulijiang River (WSL) in the northeast of China were analysed using amplified fragment length polymorphism (AFLP) markers. The results showed that 541 polymorphic loci out of 559 were amplified by 12 primer pairs and the percentage of polymorphic loci was 96.78%. Shannon indices for the MD, YL and WSL populations were 0.3988±0.2913, 0.3254±0.3037, 0.2125±0.2862, respectively, and Nei's gene diversity indices were 0.2737±0.2062, 0.2229±0.2129, 0.1446±0.1985, respectively. The average total genetic diversity (Ht) was 0.3512±0.0.0208 and the average genetic diversity within populations (Hs) was 0.2137±0.0152. Among the three populations, the average genetic distance (Dst) was 0.1375 and the gene differentiation coefficient (Gst) was 0.3914. The genetic diversity was 60.85% within populations and 39.15% among populations. The gene flow index (Nm) was 0.7776. The analysis of molecular variance (AMOVA) indicated that the average fixation index (Fst) was 0.55336. The variance was 55.16% within populations and 44.84% among populations. The highest polymorphism ratio was in the MD group and the lowest in the WSL group.
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990106
      Issue No: Vol. 6, No. 3 (2009)
       
  • Transmissible+gastroenteritis+virus+and+development+of+ELISA+based+on+the+expressed+protein&rft.title=Chinese+Journal+of+Agricultural+Biotechnology&rft.issn=1479-2362&rft.date=2009&rft.volume=6&rft.spage=249&rft.epage=252&rft.aulast=Zhen-Hui&rft.aufirst=Song&rft.au=Song+Zhen-Hui&rft.au=Guo+Wan-Zhu,+Zhang+Ying-Jun&rft_id=info:doi/10.1017/S1479236209990520">Prokaryotic expression of nucleoprotein gene of Transmissible
           gastroenteritis virus and development of ELISA based on the expressed
           protein
    • Authors: Song Zhen-Hui; Guo Wan-Zhu, Zhang Ying-Jun
      Pages: 249 - 252
      Abstract: The recombinant PET-N plasmid, which includes the N gene of the Transmissible gastroenteritis virus (TGEV), was transformed into Escherichia coli BL21 (DE3), and induced at 37°C with 1.0 mmol/l IPTG (isopropyl β-d-1-thiogalactopyranoside). An indirect enzyme-linked immunosorbent assay (ELISA) for detecting the TGEV nucleocapsid protein antibody was developed after the reactogenicity of the recombinant protein was demonstrated by Western blot. The operating conditions for the ELISA, an antigen concentration of 15 μg/ml, serum dilution of 1:40, blocking solution of 0.5% fetal bovine serum (FBS), serum sample incubation for 90 min, a concentration of horseradish peroxidase (HRP)-spa of 1:5000, incubated for 60 min, incubation of the substrate at room temperature for 10 min, and a cutoff value for the ELISA OD450⩾0.35, were carried out using a checkerboard titration method. The sensitivity and specificity of this method relative to the Svanova TGEV/PRCV antibody diagnosis kit were 93.5% and 93.8%, respectively.
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990520
      Issue No: Vol. 6, No. 3 (2009)
       
  • Purification and antibacterial activity of recombinant human lactoferrin
           in milk of transgenic mice
    • Authors: Miao Ming-Xing; Yuan Yu-Guo, An Li-You, Zhao Jun-Hui, Bai Ya-Jun, Guo Lei, Cheng Yong
      Pages: 253 - 256
      Abstract: To verify its antibacterial activity, recombinant human lactoferrin (rhLF) was extracted from the milk of transgenic mice (Mus musculus) (PCL25 and AP) by gel filtration chromatography and analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot and enzyme-linked immunosorbent assay (ELISA). In addition, its bacteriostatic properties were tested using the agar disc diffusion method. ELISA analysis showed that the concentration of rhLF in the milk of transgenic mice ranged from 7 to 8 mg/ml, and the recombinant protein expressed in the milk had the same molecular weight as the native protein (~78 kDa), indicating that the rhLFs had a strong antibacterial activity on Escherichia coli and Salmonella.
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990040
      Issue No: Vol. 6, No. 3 (2009)
       
  • Effect of nitric oxide on Sertoli cell microtubule of piglets
    • Authors: Yang Li; Wang Xian-zhong, Yang Meng-bo, Zhang Jia-hua
      Pages: 257 - 263
      Abstract: To illustrate the effect of nitric oxide (NO) on the microtubules of Sertoli cells (SC), SCs of piglets were treated with sodium nitroprusside (SNP). Changes in cell viability, anti-oxidant activity, enzyme activity and p38 mutagen-activated protein kinase (p38MAPK) activation were detected. The results were as follows. A low concentration of NO can keep SC microtubule and cell viability normal, and a high concentration of NO could increase p38MAPK activation, decrease anti-oxidant activity and transferrin secretion, and destroy the structure and distribution of the microtubules. The results suggest that SNP treatment results in an increase in NO in SCs and decreased cell anti-oxidant activity. The high concentration of NO destroys cell microtubules by activating p38MAPK.
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990064
      Issue No: Vol. 6, No. 3 (2009)
       
  • Cichlasoma+trimaculatum+(male),+Heros+managuense+(female)+and+their+hybrid+offspring&rft.title=Chinese+Journal+of+Agricultural+Biotechnology&rft.issn=1479-2362&rft.date=2009&rft.volume=6&rft.spage=265&rft.epage=270&rft.aulast=You-Ling&rft.aufirst=Chen&rft.au=Chen+You-Ling&rft.au=Zhang+Qiu-Jin,+Wang+Yan-Yin,+Mao+Jian-Ping&rft_id=info:doi/10.1017/S1479236209990118">Chromosomes of Cichlasoma trimaculatum (male), Heros managuense (female)
           and their hybrid offspring
    • Authors: Chen You-Ling; Zhang Qiu-Jin, Wang Yan-Yin, Mao Jian-Ping
      Pages: 265 - 270
      Abstract: The F1 hybrid offspring of the distant hybridization between Cichlasoma trimaculatum (male) and Heros managuense (female) were successfully obtained through artificial fertilization, and the chromosome specimen of kidney cells of the two parents and their offspring were prepared using a low colchicine concentration air drying method, Howell and Black's (1980)silver–nucleolar organizer region (Ag–NOR) staining method and Sumner's (1972)C-band staining method. The karyotypes, Ag–NORs and C-bands of C. trimaculatum, H. managuense and their offspring were observed. The results showed that the diploid numbers of the two parents and the F1 hybrid offspring were all 2n=48. For C. trimaculatum, the karyotype formula was 2n=2m+8sm+26st+12t, NF=58. Two Ag–NORs were seen on the tips of the short arm of chromosome st7, and the entire chromosomes of st9 and st13 and the centromeres of the other chromosomes had positive C-bands. For H. managuense, the karyotype formula was 2n=2m+6sm+28st+12t, NF=56. Two Ag–NORs were located on the tips of the short arm of chromosome sm2 and positive C-bands were found in the centromere of all chromosomes. In the F1 hybrid offspring, the chromosome formula was 2n=2m+8sm+30st+8t, NF=58. Two Ag–NORs were seen on the tips of the short arm of chromosome sm2; all chromosomes presented positive C-bands in the centromere. No heteromorphic sex chromosome was found. After comparison with the two parents, the F1 hybrid offspring was suggested to be the filial generation of the two hybrid parents rather than the gynogenesis of H. managuense induced by heterogeneous sperm of C. trimaculatum.
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990118
      Issue No: Vol. 6, No. 3 (2009)
       
  • Gymanadenia+conopsea&rft.title=Chinese+Journal+of+Agricultural+Biotechnology&rft.issn=1479-2362&rft.date=2009&rft.volume=6&rft.spage=271&rft.epage=276&rft.aulast=Yuan&rft.aufirst=Liu&rft.au=Liu+Yuan&rft.au=Meng+Guo-Quan,+Zhou+Jian-Ping,+Zhang+Teng,+Feng+Juan,+Ren+Zheng-long&rft_id=info:doi/10.1017/S1479236209990155">Expression, purification and identification of gibberellin-induced
           cysteine-rich protein of Gymanadenia conopsea
    • Authors: Liu Yuan; Meng Guo-Quan, Zhou Jian-Ping, Zhang Teng, Feng Juan, Ren Zheng-long
      Pages: 271 - 276
      Abstract: Primers bearing restriction enzyme sites for EcoR I and Hind III were designed according to the known partial cDNA sequence for gibberellin-induced cysteine-rich protein and were then used to amplify the full-length open reading frame (ORF) and signal peptide-truncated fragment of the gcgasa gene. Two fragments with lengths 319 and 238 bp were obtained and were further cloned into plasmid pET-32(a). Following transformation into Escherichia coli BL21(DE3), the fusion proteins were observed to appear at ~26.0 and 25.2 kDa after induction from 1 mmol/l isopropyl-beta-D-thiogalactopyronoside (IPTG). The results of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and transmission electron microscopy (TEM) of an ultra-thin section revealed that the presence of signal peptide gave rise to the formation of an inclusion body located in the periplasmic space; however, the absence of signal peptide greatly enhanced the solubility of the target protein. The expressed soluble protein was further purified by Ni2+-NTA affinity chromatography and gel filtration methods.
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990155
      Issue No: Vol. 6, No. 3 (2009)
       
  • Effect of retinoid X receptor α (RXRα) on apoptosis in primary cultured
           porcine pre-adipocytes
    • Authors: Lin Ya-Qiu; Zhuang He-Lin, Yang Gong-She
      Pages: 277 - 283
      Abstract: Using semi-quantitative reverse transcriptase polymerase chain reaction (semi-qRT-PCR), cell transfection, Hoechst 33342, acridine orange (AO) and flow cytometry methods, apoptotic changes in porcine pre-adipocytes treated with 9-cis retinoic acid (9-cisRA)–retinoid X receptor α (RXRα) ligand, transfected with pEnhanced green fluorescent proteins C2–RXRα (pEGFPC2–RXRα) or RXRα–small interfering RNA (RXRα–siRNA), respectively, were investigated. Observations of morphologic changes showed changes in cell structure, cellular shrinkage and condensation of chromatin, pyknosis of the nucleus into a compact body, complete fragmentation within the nuclear membrane, continuous budding and shedding, and, finally conversion of the cell into several apoptotic bodies wrapped in membranes of different sizes. Compared with the control group, the apoptotic rate was significantly lowered in the 10 nmol/l 9-cisRA-treated group, and significantly higher in the 10 μmol/l 9-cisRA-treated group (both P
      PubDate: 2009-12-01T00:00:00.000Z
      DOI: 10.1017/S1479236209990076
      Issue No: Vol. 6, No. 3 (2009)
       
 
 
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