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Publisher: Science and Education Publishing   (Total: 72 journals)

American J. of Applied Mathematics and Statistics     Open Access   (Followers: 3)
American J. of Applied Psychology     Open Access   (Followers: 12)
American J. of Biomedical Research     Open Access   (Followers: 3)
American J. of Cancer Prevention     Open Access   (Followers: 7)
American J. of Civil Engineering and Architecture     Open Access   (Followers: 20)
American J. of Clinical Medicine Research     Open Access   (Followers: 6)
American J. of Educational Research     Open Access   (Followers: 36)
American J. of Electrical and Electronic Engineering     Open Access   (Followers: 12)
American J. of Energy Research     Open Access   (Followers: 7)
American J. of Environmental Protection     Open Access   (Followers: 5)
American J. of Epidemiology and Infectious Disease     Open Access   (Followers: 3)
American J. of Food and Nutrition     Open Access   (Followers: 5)
American J. of Food Science and Technology     Open Access   (Followers: 5)
American J. of Infectious Diseases and Microbiology     Open Access   (Followers: 15)
American J. of Information Systems     Open Access   (Followers: 2)
American J. of Materials Engineering and Technology     Open Access  
American J. of Materials Science and Engineering     Open Access   (Followers: 1)
American J. of Mathematical Analysis     Open Access  
American J. of Mechanical Engineering     Open Access   (Followers: 2)
American J. of Medical and Biological Research     Open Access   (Followers: 3)
American J. of Medical Case Reports     Open Access  
American J. of Medical Sciences and Medicine     Open Access  
American J. of Medicine Studies     Open Access  
American J. of Microbiological Research     Open Access  
American J. of Modeling and Optimization     Open Access   (Followers: 2)
American J. of Nanomaterials     Open Access   (Followers: 1)
American J. of Numerical Analysis     Open Access  
American J. of Pharmacological Sciences     Open Access   (Followers: 1)
American J. of Public Health Research     Open Access   (Followers: 16)
American J. of Rural Development     Open Access   (Followers: 1)
American J. of Sensor Technology     Open Access   (Followers: 1)
American J. of Sports Science and Medicine     Open Access   (Followers: 6)
American J. of Water Resources     Open Access   (Followers: 1)
American J. of Zoological Research     Open Access  
Applied Ecology and Environmental Sciences     Open Access   (Followers: 13)
Automatic Control and Information Sciences     Open Access  
Biomedical Science and Engineering     Open Access   (Followers: 1)
Chemical Engineering and Science     Open Access   (Followers: 3)
Intl. J. of Celiac Disease     Open Access   (Followers: 2)
Intl. J. of Dental Sciences and Research     Open Access  
Intl. J. of Econometrics and Financial Management     Open Access  
Intl. J. of Physics     Open Access  
Intl. Transaction of Electrical and Computer Engineers System     Open Access  
J. of Automation and Control     Open Access   (Followers: 1)
J. of Biomedical Engineering and Technology     Open Access  
J. of Business and Management Sciences     Open Access  
J. of Cancer Research and Treatment     Open Access   (Followers: 1)
J. of Computer Networks     Open Access  
J. of Computer Sciences and Applications     Open Access  
J. of Environment Pollution and Human Health     Open Access   (Followers: 3)
J. of Finance and Accounting     Open Access  
J. of Finance and Economics     Open Access   (Followers: 7)
J. of Food and Nutrition Research     Open Access   (SJR: 0.344, h-index: 11)
J. of Food Security     Open Access  
J. of Geosciences and Geomatics     Open Access  
J. of Materials Physics and Chemistry     Open Access  
J. of Mathematical Sciences and Applications     Open Access   (Followers: 1)
J. of Optoelectronics Engineering     Open Access  
J. of Polymer and Biopolymer Physics Chemistry     Open Access   (Followers: 2)
Materials Science and Metallurgy Engineering     Open Access   (Followers: 1)
Nanoscience and Nanotechnology Research     Open Access   (Followers: 1)
Physics and Materials Chemistry     Open Access  
Research in Plant Sciences     Open Access  
Research in Psychology and Behavioral Sciences     Open Access  
Sustainable Energy     Open Access  
Turkish J. of Analysis and Number Theory     Open Access   (Followers: 1)
Wireless and Mobile Technologies     Open Access  
World J. of Agricultural Research     Open Access   (Followers: 1)
World J. of Analytical Chemistry     Open Access  
World J. of Chemical Education     Open Access  
World J. of Environmental Engineering     Open Access   (Followers: 1)
World J. of Organic Chemistry     Open Access  
Journal Cover   American Journal of Infectious Diseases and Microbiology
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   ISSN (Print) 2328-4056 - ISSN (Online) 2328-4064
   Published by Science and Education Publishing Homepage  [72 journals]
  • Topoisomerases II Mutations in Ciprofloxacin-resistant Clinical Isolates
           of Pseudomonas aeruginosa in Makassar, Indonesia

    • Authors: Irene E.Rieuwpassa; Ressy Dwiyanti, Yadi Yasir, Muhammad Sabir, Mochammad Hatta
      Pages: 91 - 94
      Abstract: Background: Ciprofloxacin constitute a clinically successful and widely used class of broad-spectrum antibiotics; however, the emergence and spread of resistance increasingly limits the use of this fluoroquinolone in the treatment and management of microbial disease. Ciprofloxacin is the most frequently used member of the fluoroquinolones during initial eradication therapy of Pseudomonas aeruginosa. The emergence of ciprofloxacin-resistant Pseudomonas aeruginosa (P. aeruginosa) has been reported. In this study, we examined mutations in the quinolone resistance-determining regions of the topoisomerase II (GyrA) genes of 11 clinical isolates of P. aeruginosa that resistance to ciprofloxacin. Methods: In this study we examined the relationship between gene mutations of topoisomerase II (gyrA) with ciprofloxacin resistance of P. aeruginosa. The examination performed by disc diffusion test followed by RFLP-PCR. Results: The results of disc diffusion test showed that 13 samples were sensitive to ciprofloxacin, 11 samples were resistant to ciprofloxacin. In this study we found mutation in GyrA in 6 (54.5%) of ciprofloxacin resistant samples. There was no mutation found in ciprofloxacin sensitive samples. Conclusion: Mutation in topoisomerases genes are the main mechanism of fluoroquinolone resistance in P. aeruginosa.
      PubDate: 2015-05-07
      Issue No: Vol. 3, No. 3 (2015)
  • Characterization of Some Genotypic and Phenotypic Traits of Biofilm
           Producing Clinical Isolates of Methicillin Resistant Staphylococcus

    • Authors: Malaka Farouk El Moufti; Manal Baddour, Reem Abdel Hameed Harfoush, Hoda Mohamed Aly Owais
      Pages: 95 - 103
      Abstract: Staphylococcus epidermidis is a major commensal bacterium. Various strains of methicillin resistant S. epidermidis are capable of forming biofilms and it is found to be associated with many hospital-acquired infections. Bacterial biofilms, which are micro-colonies encased in extracellular polysaccharide material, mediated by gene products of the icaADBC operon, are the sources of many bacterial infections which is so difficult to respond to routine treatments. In this research, we investigated the biofilm forming capacity of a 100 methicillin resistant staphylococcus epidermidis isolates, isolated from different clinical specimens delivered to the Diagnostic Microbiology Laboratories and Surveillance Laboratory, Faculty of Medicine, Alexandria University, in relation to the icaADBC gene cluster. Also, the minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) assays were used to evaluate the antibiotic sensitivity patterns of these MRSE isolates in their planktonic and biofilm phases to Vancomycin and Linezolid. The results showed that only 27 isolates (27%) produced detectable biofilm, and icaADBC gene was detected in only 5 of these isolates. Moreover, there was no statistical association between the presence of the gene and the biofilm status. All 27 biofilm producing isolates were susceptible to both Vancomycin and Linezolid in their planktonic state, but the MBEC values of Vancomycin were higher than those of Linezolid in almost all strains, with an agreement between both MBEC values in 15/27 (55.5%) of isolates and disagreement in 12/27 (44.5%) of isolates, and this was statistically significant (p
      PubDate: 2015-05-12
      Issue No: Vol. 3, No. 3 (2015)
  • The Challenge to Interpret Antimicrobial Susceptibility at a Small
           Community Hospital

    • Authors: Patricia Sealy; William H. Swanston
      Pages: 104 - 111
      Abstract: Objective: We aimed to determine the prevailing resistance rates at a local community hospital for a select group of gram-positive and gram-negative microorganisms for the period 2008-2012. Methods: Aerobic and facultative anaerobic culture isolates representing blood, urine and wounds specimens from all wards were tested at the Microbiology Laboratory, using the MicroScan AutoScan4, to determine antimicrobial susceptibility at the local community hospital. Results: the findings allude to the prevalence of resistance at the institution for the period under review. Conclusion: Resistance trends are speculative at best without the inclusion of molecular characterization of resistance and consumption data. Pharmacists should play a greater role in the determination of resistance by providing the microbiologist with consumption or utilization data, such as the daily defined dose. The inclusion of the daily defined dose in the estimation of resistance would allow clinicians to determine if selective pressure may have contributed to the prevailing resistance rates.
      PubDate: 2015-05-15
      Issue No: Vol. 3, No. 3 (2015)
  • Isolation and Characterization of Microbial Contamination from Computer
           Accessories used in Different Department of Hazara University and
           Diagnostic Laboratories of District Mansehra, Pakistan

    • Authors: Zeba Gul Burki; Mukhtiar Hassan, Faiza Naseer, Sohail Ahmad, Samiullah Burki, Mohammad Saleem, Attiya Nazish, Shafiq-ur- Rehman
      Pages: 112 - 124
      Abstract: 150 samples were collected from computer accessories used in Hazara University and different diagnostic laboratories of Mansehra, examined for the total bacterial count and maximum growths were observed. Samples were analyzed for further identification of micro-organisms such as E.coli, Klebsiella, S taph. aureus and Staph. epidermidis . These organisms were detected in the percentage of 46.66 of E. coli, 20% of Klebsiella, 16.66% of S. aureus & 16.66% of S. Epidermidis and identified on selective media, i.e. EMB agar and Mannitol salt agar. Furthermore, biochemical tests, including IMVIC Test, Catalase Test and Coagulase Tests were performed to confirm the presence of micro-organisms and their susceptibility also checked against different standard antibiotics and their zone of inhibitions were measured and noticed. E.coli showed maximum resistance of 97.36% against Erythromycin, Klebsiella showed against Amoxil + Clavolunic acid about 83.83%, Staph aureus showed against Erythromycin about 64.64% and Staph. e pidermidis resistance was 90.9% against Erythromycin and Gentamycin. E.coli and Klebsiell a showed maximum sensitivity for Meropenem 67.22% and 72.72% respectively while S taph. aureus and Staph. epidermidis maximum sensitivity for Vancomycin about 82.82% and 72.72% respectively. These results indicate that the computer accessories might act as environmental vehicles for the transmission of potentially pathogenic bacteria in our surroundings and also indicate the need for increasing awareness among computer users on cleaning of such surfaces or disinfection and adequate hand-washing hygiene.
      PubDate: 2015-05-22
      Issue No: Vol. 3, No. 3 (2015)
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