for Journals by Title or ISSN
for Articles by Keywords

Publisher: Indian Council of Agricultural Research   (Total: 6 journals)

Fishery Technology     Open Access   (Followers: 1)
Indian Horticulture     Full-text available via subscription   (Followers: 9)
Indian J. of Agricultural Sciences     Open Access   (Followers: 5, SJR: 0.276, h-index: 15)
Indian J. of Animal Sciences     Open Access   (Followers: 5, SJR: 0.23, h-index: 14)
Indian J. of Fisheries     Open Access   (Followers: 2, SJR: 0.222, h-index: 4)
Indian Phytopathology     Open Access   (Followers: 2)
Journal Cover   Indian Phytopathology
  [2 followers]  Follow
  This is an Open Access Journal Open Access journal
   ISSN (Print) 0367-973X - ISSN (Online) 2248-9800
   Published by Indian Council of Agricultural Research Homepage  [6 journals]
  • Influence of time of sowings and groundnut varieties on the incidence and
           severity of late leaf spot (Phaeoisariopsis personata) of groundnut

      Abstract: The field experiment was conducted to study the influence of sowing dates and groundnut varieties on the late leaf spot disease incidence, severity, corresponding defoliation, frequency and size of the leaf spot revealed that all these aspects were significantly influenced by the sowing dates and crop varieties which directly affected the pod yield and test weight. The crop sown early on 25th June exhibited maximum late leaf spot disease incidence (range 46.45-56.25%), disease severity (range 40.12-49.58%), defoliation (range, 13.18-18.36%), frequency (range, 1.38-1.76/mm2) and size (range, 1.44-1.52/mm2) followed by the crop sown on 10th June and 25th July. Among the groundnut cultivars, susceptible Cv. JL-24 exhibited maximum disease incidence (range, 35.83-56.25%), disease severity (range, 31.07-49.58%), defoliation (range, 7.70-18.36%), frequency (range, 1.21-1.76/mm2) and size (range, 1.29-1.52/mm2), followed by TAG-24, TG-26 and LGN-1. The late leaf spot disease incidence, severity and corresponding defoliation recorded at various intervals were found to be increased with the age of the crop and both were found maximum at the physiological maturity of the crop. The results obtained in respect of the influence of sowing dates and crop varieties on pod yield and test weight indicated that the crop sown early (25th June) was affected severely with the disease and thereby gave reduced pod yield (range, 1197-1679 kg/ha) and test weight (25.19-29.40 g) followed by the crop sown on 10th July and 25th July. The epidemiological implications of the disease occurring simultaneously are important because the establishment of the disease management strategies depends on the disease interactions.
      Issue No: Vol. 68, No. 3
  • Colletotrichum truncatum and C. fructicola are responsible for the cause
           of anthracnose on chilli in Karnataka state of India

      Abstract: Chilli is an important spice as well as vegetable crop in India. A anthracnose is one of the most devastating diseases wherever chillies are grown under warm and wet season. The disease appears as sunken necrotic lesions with concentric rings of acervuli on fruit. Colletotrichum species responsible for severe outbreaks of anthracnose on chillies in Karnataka state of India are reported. Forty nine isolates recovered from major chilli production areas of Karnataka state were characterized based on morphological and multilocus gene phylogeny using partial ITS, ACT, CHS-1, GAPDH, CYLH3, TUB2, GS and ApMat genes. The results showed that presence of two species, C. truncatum (37 isolates) and C. fructicola (12 isolates). Pathogenecity assays of the two species were confirmed on ‘chilli cv Arka Lohith’. Thus, the results of this study can be utilized for devising disease management strategies and for the development of disease resistant varieties for use in commercial production of chilli in India.
      Issue No: Vol. 68, No. 3
  • Evaluation of plant extracts for induction of defence against Fusarium
           wilt in chickpea

      Abstract: In the present investigations, effect of six plant parts extracts viz., leaf extracts of Calotropis procera, Eucalyptus globulus and Withania somnifera, fruit extract of Citrullus colocynthis and leaf and seed kernel extracts of Azadirachta indica was assayed on host defense triggery mechanism. The activities of soluble phenol, and protein contents, peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase, β-1, 3-glucanase and chitinase activities in chickpea roots were observed. The effect of plant extracts on wilt incidence, seed germination and plant growth parameters viz., root and shoot lengths and dry weight in chickpea and the population of Fusarium oxysporum f. sp. ciceri in soil was also recorded under greenhouse condition. Seed treatment and soil application of aqueous extracts of A. indica seed kernel, C. colocynthis fruit and A. indica leaf significantly increased the soluble phenol, and protein contents and the activities of peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase, β-1, 3-glucanase and chitinase activities in chickpea roots. Seed treatment and soil application of aqueous extracts of A. indica seed kernel, C. colocynthis fruit and A. indica leaf reduced the wilt incidence of Fusarium wilt, population of F. oxysporum f. sp. ciceri in soil and increased root and shoot lengths and dry weight of chickpea plants.
      Issue No: Vol. 68, No. 3
  • Assessment of efficacy of pesticides applied singly or combinations on
           rice sheath blight disease development

      Abstract: Efficacy of commonly used pesticides namely carbendazim (0.1%), hexaconazole (0.1%), endosulfan (0.042%), chlorpyriphos (0.02%), thiobencarb (0.3%) and butachlor (0.4%) applied singly or in combination were evaluated  against rice sheath blight (Rhizoctonia solani), during kharif 2010 and 2011 under pot culture conditions. Among the various pesticide combinations, carbendazim (0.1%) applied along with endosulfan (0.042%) was found to be the most effective against sheath blight, where the lowest mean disease severity was 17.36% and the relative vertical spread (RVS) was 14.18%. This combination increased the grain yield and straw yield by 85.68% and 111.73%, respectively in comparison to inoculated control without any pesticide. This was followed by hexaconazole (0.1%) combined with endosulfan (0.042%), where the mean disease severity was 19.84% and the relative vertical spread was 16.34%. It increased the grain yield by 72.21% and straw yield by 104.37% when compared to R. solani inoculated control without any pesticide. In case of individual application of carbendazim, the mean disease severity and relative vertical spread were observed to be 24.81% and 22.37%, respectively. Per cent increase in grain yield and straw yield were recorded as 29.86% and 61.94%, respectively. Treatment with hexaconazole alone reduced the mean disease severity up to 27.71% and relative vertical spread of 25.06%. It increased grain yield up to 28.10% and straw yield up to 60.35%. Thus, it is evident that, combined application of pesticides not only shows higher efficacy in controlling the disease, but also increases grain yield and straw yield.
      Issue No: Vol. 68, No. 3
  • Bacillus megaterium from tea rhizosphere promotes growth and induces
           systemic resistance in tea against Sclerotium rolfsii

      Abstract: The objectives of the study was to determine how Bacillus megaterium, isolated from tea rhizosphere promotes the growth of tea plants as plant growth promoter, affects sclerotial blight disease and induces systemic resistance in tea plants. Bacillus megaterium, promoted growth of 5 varieties of tea, as evidenced by increase in height, emergence of new leaves, branches and increase in leaf numbers both in potted conditions and in the field. The bacterium was antagonistic to a number of fungal pathogens in vitro and reduced sclerotial blight of tea caused by Sclerotium rolfsii. Significant increases in phenolics along with increase in isomers of catechins as well as peroxidase, chitinase, β-1,3-glucanase and phenylalanine ammonia-lyase, were observed in tea plants on application of B. megaterium alone or following challenge inoculation with S. rolfsii. Survival of B. megaterium in soil was determined by ELISA and Dot-Blot using PAb raised against the bacterium. Enhanced expression of chitinase in B.megaterium treated leaves was confirmed by Western Blot and Dot Blot using PAb raised against chitinase. Besides, immunolocalization of chitinase in tea leaves was also done using the chitinase PAb. B. megaterium has been found to be a good plant growth promoter with the ability to increase growth of tea plants along with a reduction of sclerotial blight disease which acts both by direct and indirect mechanisms in the host.
      Issue No: Vol. 68, No. 3
  • Management of fusarium wilt of tomato under protected conditions using
           brassica crop residues, AM fungi and biocontrol agents

      Abstract: Fusarium wilt of tomato was managed using three fungal, viz. Trichoderma viride, T. hamatum, T. harzianum and a bacterial viz. Pseudomonas flourescens biocontrol agents in vitro. Four different genera of arbuscular-mycorrhizal (AM) fungi viz., Glomus sp., Acaulospora sp., Scutellospora sp. and Gigaspora sp. were isolated from tomato soil and their culture consortium was multiplied on Guinea grass in pots. Under in vitro condition, T. viride was found most effective in inhibiting the mycelial growth of F. o. lycopersici. Amongst different bio-fumigant crop residues (fresh as well as dry) tested in vitro, taramira showed highest inhibition in mycelial growth of F. o. lycopersici i.e. 62.59 and 60.74 per cent, respectively. All the effective management inputs evaluated under in vitro conditions were again evaluated under polyhouse in an integrated manner. The results of polyhouse experiment indicated that bio-fumigation of sick soil with taramira crop residues for 30 days, and application of formulation of T. viride along with FYM and inoculation of transplants with indigenous AM fungi resulted in to more than 80%-control of Fusarium wilt of tomato under polyhouse conditions. This practice also increased the growth viz., height, number of branches and plant dry weight as well as yield parameters like number of fruits per plant and fruit yield per plot. Colony forming units (cfu/g) of Fusarium sp. were decreased while that of Trichoderma sp. increased in soil owing to treatment of soil with bioinoculants. The spore population of AM fungi was found more in the plots receiving this treatment as compared to control.
      Issue No: Vol. 68, No. 3
  • Screening of potato (Solanum tuberosum) cultivars against common scab
           (Streptomyces scabies) and their management through biofumigation

      Abstract: Common scab (Streptomyces scabies) disease of potato is becoming predominent in many potato growing districts of Gujarat state resulting in economic losses, since a decade. A field experiment was conducted during 2011-2013 on 17 potato cultivars for screening against common scab disease at Potato Research Station, SDAU, Deesa, Gujarat. None of the cultivars were found resistant. However, 10 cultivars (Kufri Frysona, Kufri Khyati, Kufri Bahar, Kufri Sadabahar, Kufri Pushkar, CP-2323, CP-2030, CP-2370, CP-1588 and CP-2378,) recorded moderately resistant reaction to common scab. For the management of this disease, an experiment was conducted during 2009-2012 with cv. Kufri Badshah with seven treatments evolving eco-friendly components, viz. bio-formulations (Trichoderma viride and Bacilus subtilis strain B5), mustard biofumigation as green manuring, seed tuber treatment with boric acid (3% spray) and gypsum and bleaching powder. Minimum common scab incidence during all the three years was 33.55, 26.79 and 29.79 per cent in biofumigation with mustard as green manuring one month before sowing, respectively.
      Issue No: Vol. 68, No. 3
  • Evaluation of pre-symptom activity of fungicides against Marssonina
           coronaria causing premature leaf fall in apple

    • Authors: ANIL KUMAR*; J.N. SHARMA
      Abstract: Fifteen fungicides were evaluated for their pre-symptom activities against Marssonina coronaria under polyhouse during causing premature leaf fall in apple 2009 and 2010. Mancozeb, captan, copper oxychloride, metiram, benomyl, carbendazim, thiophanate methyl, Shield, Kresoxim methyl, azoxystrobin, pyraclostrobin, hexaconazole, difenoconazole and tebuconazole were tested at 0.1, 0.2 and 0.3% concentration, whereas Dodine at 0.05, 0.075 and 0.1%. Pot plants of apple cv Starking Delicious were inoculated with standardized conidial suspension (5 × 104 conidia ml-1) of M. coronaria and plants were treated 9 days after inoculation. Timing of application was based on incubation period (12 days) of M. coronaria on the test cultivar. Strobilurins (kresoxim methyl, pyraclostrobin and azoxystrobin) had strongest pre-symptom activity against the disease in polyhouse exhibited 70.99-91.08% control during both the years. These fungicides also had highest inhibitory effect on conidial production (4.25 × 104 conidia ml-1 - 5.54 × 104 conidia ml-1) and reduced lesion size (3-6 mm) produced on the treated leaves compared to other treatments. Captan was least effective against Marssonina blotch of apple among all the treatments exhibited 41.16-49.58% disease control during both the years. Kresoxim methyl exhibited highest disease control (80.82-91.08%) among all the fungicides. It could be used for effective management of Marssonina blotch in apple.
      Issue No: Vol. 68, No. 3
  • Isolation and characterization of Pseudomonas spp. and their evaluation in
           management of replant problem in apple

      Abstract: Under this study isolation and characterization of 30 native fluorescent Pseudomonas isolates by phenotypically (fluorescence, oxidase, catalase test, gelatin liquification, denitrification test, gelatin liquification, MR/VP, citrate utilization, arginine dihydrolysis) and genotypically by 16S rRNA from normal and replant site of apple and pear orchard was done. These were screened out for multifarious plant growth promoting activities like production of plant growth regulators, phosphate solubilization, antifungal, siderophores, HCN, ammonia and lytic enzymes. Auxins, gibberellins and cytokinins produced by isolates were in the range of 1.83-21μg/ml, 116.1-485.8 μg/ml and 63.6-90.0 μg/ml, respectively. Pseudomonas isolates showed production of phosphate solubilizing activity in the range of 199.5 to 413.4 μg/ml available inorganic phosphate (Pi) and 67.27% SU siderophore production. Three Pseudomonas isolates (An-1-kul, An-13-kul and An-3-kul) showed maximum antifungal activities. In this study the effect of inoculation of apple (Malus domestica Brokh.) rhizosphere with plant growth-promoting rhizobacteria (PGPR) i.e. Pseudomonas strain was evaluated. The control treatment was non-inoculated and the PGPR treatment was inoculated with three strains of Pseudomonas sp.An-1-kul, An-13-kul and An-3-kul were used individually and their consortia for treatment of apple in replant field. The performance of apple seedlings was much better in terms of plant establishment, growth promotion in terms of plant height, number of nodes and number of branches, chlorophyll content of leaves and NPK of rhizospheric soil over their respective control. These strains can be further exploited and recommended for the management of replant problem of apple after conducting few more trials in replant site.
      Issue No: Vol. 68, No. 3
  • Evaluation of molecular markers in diversity analysis among isolates of
           Lasiodiplodia theobromae Pat. (Griffon & Maubl) inciting root and
           collar rot of biofuel plant (Jatropha curcas)

      Abstract: Jatropha (Jatropha curcas L.) plantation is highly threatened by root rot and collar rot caused by Lasiodiplodia theobromae Pat. (Griffon & Maubl). Nine isolates of Lasiodiplodia theobromae collected from jatropha from various regions in Gujarat and Madhya Pradesh were characterized based on their molecular profiles of the isolates. Clustering pattern of dendrogram generated by using the pooled molecular data of 14 RAPD loci formed two clusters. The highest similarity index value of 0.50 was found between Lt-5 isolate and Lt-6 isolate. The average similarity coefficient among isolates was 0.403. The data obtained from Random Amplification of Polymorphic DNA (RAPD) with 14 arbitrary primers produced 279 loci and 861 amplicons. Out of the 279 loci produced, 266 were polymorphic and hence the total polymorphism was found to be 95.60%. Average number of amplicons per primer was found to be 19.9 and average number of polymorphic loci obtained per primer (Assay Efficiency Index) was found to be 19. Out of 14 primer, 5 successfully produced 100% polymorphism successfully.
      Issue No: Vol. 68, No. 3
  • Evaluation of Pseudomonas and Trichoderma enriched organic amendment
           against Alternaria leaf spot of Withania somnifera (L.) Dunal.

    • Authors: VIBHA PANDEY*
      Abstract: Leaf blight of Ashwagandha (Withania somnifera) is the most prevalent disease in almost all the cultivated areas of the crop. Systemic fungicides and different isolates of Trichoderma spp. were tested for their efficacy against Alternaria alternata under in-vitro conditions. Similarly, bacterial and fungal bioagents were also tested for their compatibility with different cakes. The best Trichoderma isolate, Pseudomonas fluorescens and FYM were evaluated individually and in- combination, with or without spray of propiconazole, for the management of Alternaria leaf blight under field conditions. The field experiment was conducted in Factorial Randomized Block Design (FRBD) with factor A comprises of spray of propiconazol @ 0.25 per cent and without chemical spray and the factor B of biological combinations incorporated into the soil. Among the fungicides and different isolates of Trichoderma spp., propiconazole and T. viride 3 (TV3) were found as most effective in reducing the growth of the pathogen. The compatibility studies revealed that the FYM was compatible with both T. viride 3 (TV3) and P. fluorescens. The effect of propiconazole spray was statistically superior to without chemical spray on disease suppression. Similarly, the mixture of T. viride 3 (TV3) + P. fluorescens and FYM + T. viride 3 (TV3) equally reduced the disease incidence and increased the dry root and seed yield.
      Issue No: Vol. 68, No. 3
  • Assessment of genetic diversity in Ganoderma lucidum using RAPD and ISSR

      Abstract: Genetic diversity of 31 isolates/strains of Ganoderma lucidum from different hosts and geographic regions were analyzed using RAPD and ISSR markers. Ten polymorphic RAPDs were selected from 156 primers along with 8 polymorphic universal. RAPD and ISSR exhibited polymorphism of 88.2 and 78.8 per cent, respectively. RAPD markers, viz. S-1005, OPF-6 exhibited maximum polymorphism (93.3%) whereas ISSR- 833 showed maximum of 89.6 per cent polymorphism. Cluster analysis of 31 isolates/strains of G. lucidum grouped isolates in 4 subpopulation at 42 per cent (RAPD) and 5 subpopulation using ISSR markers at 40 per cent similarity coefficient. Multilocus ISSR primers clustered isolates based on their geographical origin to considerable levels and exhibited fair similarities among grouping of isolates having sessile/short stalk length than those having long stalk. Therefore, this study reports the high level of genetic diversity of Ganoderma lucidum from North Western Himalayas using both RAPD and ISSR techniques.
      Issue No: Vol. 68, No. 3
  • Epidemiological studies of stem necrosis disease in potato caused by
           Groundnut bud necrosis virus

      Abstract: Stem necrosis disease causes serious reductions in tuber yield of potato (Solanum tuberosum L.). The causal agent of this disease is Groundnut bud necrosis virus (GBNV, genus Tospovirus). It is effectively transmitted by mechanical sap and insect vector, thrips (Thrips palmi) inoculation. Association of the GBNV with infected experimental potato host plants was detected by RT-PCR using specific primer pairs that targets NP gene. In vector transmission studies, minimum acquisition feeding period was 24 hrs and the minimum inoculation access period was 48 hrs. In screening of germplasms, out of 60 different accessions 50 genotypes were infected. Majority of plant showed necrotic symptom on stem followed by veinal necrosis. Out of two geographical locations, maximum disease index of 1.89 and 1.83 was found at ARS, Kota in early ie. mid October planted tubers of two successive field experiments, respectively. The information generated will help in understanding the dynamics of disease, transmission, and its range on various germplasms, that will help to formulate the strategy to manage the disease.
      Issue No: Vol. 68, No. 3
  • Lack of resistance in cotton against cotton leaf curl begomovirus disease
           complex and occurrence of natural virus sequence variants

      Abstract: A survey was made to study incidence of cotton leaf curl disease (CLCuD) in cotton fields at Indian Agricultural Research Institute (IARI), New Delhi for the last six years from 2009 to 2014. It was observed that CLCuD has increased from initial year to current year of study. The average disease incidence was found to be as high as 15.92% in 2013 and moderate of 8.4-9.2% in 2012 and 2014, and 2.1-6.4% in the years of 2009 to 2011. Of total number of 55 cotton varieties screened against CLCuD in field condition in 2013 and 2014, none was found to be resistant. The per cent plant infection varied with the cultivars and ranged from 0 to 46.4% in 2013 and 0 to 26.4% in 2014. However, based on the field study for both the years, seven varieties, P21-51, P-31, P1752, P5616, P5618, 12 P 4 and P 5628 were identified as tolerant varieties. In greenhouse condition, one hundred thirty three cotton cultivars including 73 varieties, 48 germplasm and 12 Bt cotton hybrids were screened through whitefly inoculation. None of the cultivars were found to be resistant to this disease. All the Bt hybrids tested were found to be highly susceptible. Of 15 desi cotton (G. arboretum) varieties tested, none was infected by CLCuD. Whitefly transmission and nucleotide sequence analysis of coat protein (CP) and C2 gene determined that the causal agents of CLCuD are whitefly transmitted CLCuD-begomoviruses.  Real time-PCR analysis and the analysis of CP and C2 gene indicated appearance of virus variants at IARI cotton field.  Present study concluded the trend in increase of CLCuD in cotton over years due to lack of resistance in cotton cultivars which might be due to the occurrence of possible variants.
      Issue No: Vol. 68, No. 3
  • Sequence analysis of ribosomal protein gene of Candidatus Liberibacter
           asiaticus infecting major citrus cultivars in western Maharashtra of India

      Abstract: Citrus greening (Huanglongbing, HLB) is the most serious and destructive citrus disease known worldwide. It is caused by three different species of “Candidatus Liberibacter”, a Gram negative alpha-proteobacterium and classified on the basis of its geographical origins and 16Sr DNA sequence. During a survey conducted in the year 2010 in Maharashtra state of India, disease occurrence was recorded in different citrus species viz. sweet orange cv 'Mosambi', Rangpur lime, Nagpur mandarin, acid lime and rough lemon. The disease was found most prevalently in 'Mosambi' sweet orange cultivar and maximum disease incidence up to 41.5% was observed in Ahmednagar district. Four different citrus species viz. sweet orange cv 'Mosambi', Rangpur lime, Cleopatra mandarin and acid lime that showed variable symptoms of suspected greening disease in the field were found positive for ‘Candidatus Liberibacter spp.’ through biological indexing and PCR assays using 16S rDNA primers. PCR amplifications and sequence comparison of another well conserved region, β operon’s subunits rplA-rplJ confirmed the associated pathogen as “Candidatus Liberibacter asiaticus”. The sequence analysis of greening isolates in this geographical region revealed that all isolates were closely related to ‘Candidatus Liberibacter asiaticus’. The analysis of Single Nucleotide Polymorphisms at β- operon ribosomal protein (β-rp SNP) confirmed that irrespective of their host, all isolates strictly belong to genetic group of β-rp SNP lineage II.
      Issue No: Vol. 68, No. 3
  • Diversity for resistance to stem and leaf rusts in durum wheat (Triticum

      Issue No: Vol. 68, No. 3
  • Evaluation of fungicides against late blight (Phytophthora infestans) on
           susceptible and moderately resistant potato cultivars

      Issue No: Vol. 68, No. 3
  • Etiology, symptomatology and molecular characterization of papaya root rot
           – a new and serious threat

    • Authors: S.K. SINGH; RAHUL KUMAR*
      Issue No: Vol. 68, No. 3
  • Assessment of yield loss of cumin (Cuminum cyminum) caused by Alternaria
           leaf blight and pathogen recovery from infected seeds

      Issue No: Vol. 68, No. 3
  • Identification of resistant sources in kodo millet against Sorosporium
           paspali thunbergii and its effect on seed germination

      Issue No: Vol. 68, No. 3
  • ‘Candidatus Phytoplasma asteris’ association with little leaf
           and leaf yellows symptoms of Viola tricolor in India

      Issue No: Vol. 68, No. 3
  • Genetic variability of multifunctional HC - Pro gene of Lettuce mosaic
           virus in north region of India

      Issue No: Vol. 68, No. 3
  • Eryngium foetidum (wild coriander): A new host recorded powdery mildew
           pathogen (Oidiopsis macrospora) from India

      Issue No: Vol. 68, No. 3
School of Mathematical and Computer Sciences
Heriot-Watt University
Edinburgh, EH14 4AS, UK
Tel: +00 44 (0)131 4513762
Fax: +00 44 (0)131 4513327
About JournalTOCs
News (blog, publications)
JournalTOCs on Twitter   JournalTOCs on Facebook

JournalTOCs © 2009-2015