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Publisher: Indian Council of Agricultural Research   (Total: 6 journals)   [Sort by number of followers]

Showing 1 - 6 of 6 Journals sorted alphabetically
Fishery Technology     Open Access   (Followers: 1)
Indian Horticulture     Full-text available via subscription   (Followers: 10)
Indian J. of Agricultural Sciences     Open Access   (Followers: 7, SJR: 0.276, h-index: 15)
Indian J. of Animal Sciences     Open Access   (Followers: 5, SJR: 0.23, h-index: 14)
Indian J. of Fisheries     Open Access   (Followers: 1, SJR: 0.222, h-index: 4)
Indian Phytopathology     Open Access   (Followers: 2)
Journal Cover Indian Phytopathology
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  This is an Open Access Journal Open Access journal
   ISSN (Print) 0367-973X - ISSN (Online) 2248-9800
   Published by Indian Council of Agricultural Research Homepage  [6 journals]
  • Seed biopriming: A comprehensive approach towards agricultural
           sustainability

    • Authors: H.B. SINGH
      Abstract: Plants and microorganism are involved in a continuous network of interactions, some of those beneficial to the plants and some are detrimental. There is a rising demand for intervention of ecologically safe and sound, environmentally compatible techniques in crop production which will provide global food security and improved agricultural produces. To accomplish this goal, application of agriculturally beneficial microorganisms is a potential alternative to traditional agricultural techniques which have severely damaged the agro-ecosystem (Abhilash et al., 2016). Potential of agriculturally important microorganisms to reduces or replace the agrochemicals chemicals has been so far evaluated. Beneficial microorganisms including biological control agents (BCAs), plant growth promoting rhizobacteria (PGPRs) and fungi (PGPFs) and endophytes play a crucial role in sustainable crop production. These microorganisms provide growth promotion, crop protection and abiotic stress mitigation by the direct application. In the last decades various genera of PGPR have been identified and their application as biofertilizer and biocontrol agent has seen a great boost. Various species of bacteria including Pseudomonas, Bacillus, Azospirillum, Serratia, Azotobacter, Enterobacter, Klebsiella, Alcaligenes and Burkholderia and have been reported as potential biocontrol agents, biofertilizers and biostimulants (Keswani et al., 2014, 2015a). Various bacteria which are predominantly investigated and successfully commercialized as the biological control agents are Pseudomonas fluorescens, Bacillus subtilis, Agrobacterium radiobacter, Serratia marcescens, Streptomyces griseoviridis and Burkholderia cepacia. They suppress plant pathogen in soil through production of antibiotics and siderophores and suppress plant disease in plant through induction of defense response (Bisen et al., 2015; Keswani et al., 2015b; Singh, 2014). In addition to influencing plant health and growth, these beneficial microorganisms also strengthen the survival of plants in tolerate biotic and abiotic stress, increase uptake and nutrient availability, and improve the soil microflora diversity.
      Issue No: Vol. 69, No. 3
       
  • First report of leaf spot disease of culinary melon caused by
           Colletotrichum fructicola in India

    • Authors: S. NARMADHAVATHY*; KAMALA NAYAR, C. GOKULAPALAN D. GEETHA
      Issue No: Vol. 69, No. 3
       
  • Evaluation of different fungicides for effective management of Blumeriella
           leaf spot of cherry in Kashmir Valley

    • Authors: KAMRAN AHMED KHAN*; SAJAD UN NABI NISSAR AHMAD KHAN
      Issue No: Vol. 69, No. 3
       
  • Management of common scab (Streptomyces scabies) of potato through
           eco-friendly approach

    • Authors: RAMAKANT GHARATE; NARENDRA SINGH* S.M. CHAUDHARI
      Issue No: Vol. 69, No. 3
       
  • Response of rabi maize against common rust incited by Puccinia sorghi
           schw.

    • Authors: V.K. MALIK*; R.S. CHAUHAN
      Issue No: Vol. 69, No. 3
       
  • A new record of Fusarium oxysporum causing stem lodging, inflorescence and
           capsule rot in large cardamom

    • Authors: R. GOPI*; R.K. AVASTHE, H. KALITA, ASHISH YADAV, CHANDAN KAPOOR CHANDA POUDYAL
      Issue No: Vol. 69, No. 3
       
  • Inheritance of resistance in urdbean (Vigna mungo) to anthracnose caused
           by Colletotrichum truncatum

    • Authors: SHAYLA BINDRA*; R.K. MITTAL, V.K. SOOD P.N. SHARMA
      Issue No: Vol. 69, No. 3
       
  • Evaluation of Pseudomonas fluorescens isolates for the management of
           Fusarium oxysporum f.sp. lycopersici and Rhizoctonia solani responsible
           for causing wilt complex in tomato

    • Authors: STANZIN DORJEY; V.K. RAZDAN VISHAL GUPTA*
      Abstract: Twenty-five isolates of Pseudomonas fluorescens were isolated from healthy plants of tomato on Kings’B medium for exploring their bio-efficacy potential against Fusarium oxysporum f.sp. lycopersici and Rhizoctonia solani responsible for wilt complex disease. Out of all, six isolates (I-7, I-15, I-18, I-23, I-24 and I-25) significantly inhibited the growth of fungal pathogens under laboratory conditions. Under green house conditions, bacterization of tomato seeds with these six isolates of P. fluorescens in pathogen infested soil significantly reduced the incidence of disease caused by F. oxysporum f.sp. lycopersici and R. solani, with maximum (14.58 and 16.67%) by isolate I-23,and simultaneously increased the plant growth parameters. Application of isolate I-23 also resulted in maximum per cent germination, shoot length and root length of 80.00 and 77.50 per cent and 53.12 and 41.93 per cent, 54.00 and 38.00 cm and 16.27 and 11.97 cm, respectively as compared to their untreated control.
      Issue No: Vol. 69, No. 3
       
  • Biological control of crown gall disease in peach nursery plants by white
           stone powder based formulation (Sol Gall) of Brevibacillus spp.

    • Authors: ADITI SHARMA; A.K GUPTA*, DINESH SINGH, KISHORE KHOSLA RISHI MAHAJAN
      Abstract: Agrobacterium tumefaciens caused losses in stone fruit nurseries as present day management strategies are not satisfactorily work. In vitro analysis of the antagonistic activity of different Brevibacillus spp. towards plant tumorigenic Agrobacterium tumefaciens resulted in a selection of potential antagonistic isolates. Therefore, these isolates were also tested for their efficacy in reducing gall formation in plants and under field condition either alone or in combination. Brevibacillus brevis (UHFBA-22) and B. choshinensis (UHFBA-30) mixed in 1:1 ratio (designated as UHFBA-28), showed maximum antagonistic activity (3.87cm dia. of inhibition zone) against A. tumefaciens. UHFBA-28 when applied in the form of white stone powder based formulation (Sol Gall) as seed treatment resulted in11.11% incidence of crown gall. Root dip treatment with Sol Gall also minimized incidence of crown gall to 12.24 and 38.89 per cent as compared to 91.53 and 93.33 per cent incidence in two different experiments. However, soil drenching of nursery plants with UHFBA-28 gave complete control of crown gall disease in peaches. This treatment was comparable with other cumbersome treatments viz., soil solarization and its combination of seed treatment with UHFBA-28, and treatment of nursery soil with, formaldehyde (5%) + seed treatment with UHFBA-28.
      Issue No: Vol. 69, No. 3
       
  • Morphological and molecular characterization of Trichoderma asperellum
           strain Ta13

    • Authors: R. THAVA PRAKASA PANDIAN; M. RAJA PRATIBHA SHARMA*
      Abstract: Trichoderma spp. is used as a bio-fungicide avowed for its immense use in agriculture and industry. Morphological characters are not sufficient to properly identify different species of Trichoderma. In recent days, multi-gene phylogeny in combination with morphological characters is used to confirm the Trichoderma at species/subspecies level. The current study was focused to characterize Trichoderma asperellum strain Ta13 based on morphology and molecular analysis using genes such as ITS, tef1α, rpb2, act and cal. Light and scanning electron microscopy (SEM) results showed that Ta13 has regularly branched, typically paired conidiophores with straight phialides and globose to sub-globose shaped conidia having size of 2.91µm x 2.37µm with inconspicuous ornamentation. Phylogenetic analysis results for ITS, tef1α and rpb2 genes with reference T. asperellum showed that Ta13 formed a single cluster with other reported T. asperellum isolates and confirmed that Ta13 strain is T. asperellum. Confrontation assay results clearly showed that Ta13 inhibited several fungal plant pathogens viz. Rhizoctonia solani (88.69 per cent), Fusarium oxysporum f. sp. lycopersici (85.27 per cent), F. moniliforme (84.55 per cent), S. sclerotiorum (80.17 per cent), S. rolfsii (78.26 per cent), and A. brassicicola (77.5 per cent) very effectively and shown higher level of antagonism (Class I). Cell-free culture assay results also revealed the biocontrol potential of Ta13 by arresting the pathogens growth efficiently.
      Issue No: Vol. 69, No. 3
       
  • Management of bacterial wilt of brinjal using wild brinjal (Solanum torvum
           Sw) as root stock

    • Authors: R. RAMESH*; G. ACHARI, T. ASOLKAR, M. DSOUZA N.P. SINGH
      Abstract: Brinjal cultivation in coastal regions of India is severely affected by the incidence of bacterial wilt caused by Ralstonia solanacearum. The pathogen is highly diverse and its management is a challenging task. A wild relative, Solanum torvum was identified as resistant type. Seedlings of the cultivated, local brinjal types, Agassaim, Taleigao and other lines from a segregated population were grafted on wild type, S. torvum. None of the Agassaim grafts was found wilted when challenged with the pathogen owing to inability of the bacterium to colonize the grafts. Field evaluation of the grafts indicated the complete protection from bacterial wilt whereas the seedlings recorded 60 to 74 per cent wilt in three field trials and 66 to 84 per cent wilt in forth trial. Grafted plants yielded fruits similar to that of the seedling type indicating its acceptability among the growers and consumers. This technology could be a promising strategy in the management of bacterial wilt.
      Issue No: Vol. 69, No. 3
       
  • A comparative virulence analysis in Bipolaris sorokiniana based on host
           interaction with pathogen and its toxin

    • Authors: SAPNA SHARMA*; MEHDI JAHANI RASHMI AGGARWAL
      Abstract: Bipolaris sorokiniana is a hemibiotrophic fungus causing spot blotch of wheat and barley. The pathogen produces phytotoxins which play role in pathogenesis. Our results indicated the existence of variability based on host interactions with pathogen and toxin produced by the pathogen, separately. Twelve isolates of B. sorokiniana showed differences in disease reaction on wheat differential hosts. BS 25 was identified as most virulent and BS 7 as least virulent isolate. These two selected isolates further showed differences in the intensity of toxin band when resolved by thin layer chromatography (TLC). The grades of symptoms produced by the toxin of these isolates were similar to those produced by the respective pathogen isolates.
      Issue No: Vol. 69, No. 3
       
  • Cultural and morphological variability of isolates of Sclerotium rolfsii
           Sacc. causing stem and pod rot of groundnut

    • Authors: POORNIMA*; GURURAJ SUNKAD, HARI K. SUDINI M.K. NAIK
      Abstract: Eighty isolates of Sclerotium rolfsii collected from viz., Raichur, Ballari, Koppal, Gadag, Tumkur, Chitradurga, Kalburgi and Yaadgir districts of Karnataka were used to study cultural and morphological variability based on their radial growth, colony morphology, sclerotial production, pattern of production of sclerotial bodies, number of days taken to produce sclerotial bodies, number of sclerotial bodies produced per plate, colour, size and texture of sclerotial bodies. Significant variability with reference to mycelial and sclerotial characters across isolates of S. rolfsii isolated from different locations of Karnataka was observed. Out of eighty isolates 73 isolates were fast growing with an average growth rate 75-90 mm, five intermediate growing isolates 60-75 mm and two isolates slow growing with an average growth rate of 45-60 mm. With respect to colony morphology, fifteen isolates showed compact growth while sixty five isolates showed fluffy growth and sixty four isolates produced sclerotial bodies scattered all over the plate, thirteen isolates which produced sclerotial bodies at the periphery region whereas, isolate Sr33 and Sr34 produced sclerotial bodies at the centre of plate. Further, all eighty isolates produced sclerotial bodies except Sr77 which did not produce sclerotial bodies. Most of the isolates produced sclerotial bodies within 11-14 days except Sr60 which took 19 days and the isolate Sr15 recorded the production of more than 300 sclerotial bodies per plate.
      Issue No: Vol. 69, No. 3
       
  • Status of arbuscular mycorrhizae associated with rhizosphere of sweet
           

    • Authors: SHRUTI KIRTI*; RUCHI SHARMA, BHAWNA DIPTA, ROHIT PAWAR, RAJESH KAUSHAL NARENDER K. BHARAT
      Abstract: Arbuscular mycorrhizae (AM) have been described as a “universal symbiosis” between plants and beneficial soil microbes which increases growth, nutrient uptake and defense mechanism of various horticultural plants. In the present investigation, eight efficient indigenous AM fungi were isolated and characterized from various geographical locations viz., Dhochi, Dhar, Kandiyali and Thanedhar of Shimla district, Himachal Pradesh, India. These AM spores were identified up to species level as Glomus macrocarpum, G. mosseae, G. constrictum, G. fasciculatum, G. clarum, Acaulospora bireticulata, A. scrobiculata, Gigaspora albida and Scutellospora spp. Maximum number of spores (323.33 spores/50 g soil) and root colonization (29.33%) was recorded at Kandiyali and minimum number of spores (151.67 spores/50 g soil) and root colonization (19.22%) was observed at Dhar.
      Issue No: Vol. 69, No. 3
       
  • Antifungal activity assessment of essential oil of bitter apricot (Prunus
           armeniaca L.) kernels

    • Authors: MONICA SHARMA*; P.C. SHARMA, KIRTI KAUNDAL HIMANSHU SHARMA
      Abstract: Essential oils of botanicals constitute a promising alternative for hazardous fungicides in integrated disease management modules. The study describes the in vitro antifungal activity of essential oil obtained from hydro-distillation of apricot kernel press cake. Four different concentrations of essential oil (5%, 10%, 15% and 20%) were screened for their antifungal effect against different phytopathogenic fungi viz. Fusarium oxysporum, Sclerotium rolfsii and Macrophomina sp. There was complete inhibition in mycelial growth of all three fungi at 15% and 20% concentrations while at 10% concentration, no growth was observed in F. oxysporum and least growth rate was recorded in S. rolfsii (1.0mm/day) followed by Macrophomina sp. (1.3mm/day). In control, the mycelia growth rate was 9.7mm/day, 9.0mm/day and 9.8mm/day for F. oxysporum, S. rolfsii and Macrophomina sp., respectively. Mycelial inhibition of 30.9%, 61.37% and 52.44% was observed for F. oxysporum, S. rolfsii and Macrophomina sp., respectively at 5% oil concentration in the medium while at 10 % oil concentration, level of inhibition was increased to 100.0%, 88.9% and 86.76% for F. oxysporum, S. rolfsii and Macrophomina sp., respectively. Seed treatment with bitter apricot oil at 5% and 10 % concentration effectively managed the Fusarium wilt disease of pea under field conditions and significantly improved the yield. These findings suggest that essential oil of bitter apricot kernel press cake has antifungal activity and may be used in formation of biopesticide for managing diseases in many crops.
      Issue No: Vol. 69, No. 3
       
  • Molecular characterization and influence of soil factors on Rhizoctonia
           solani in Meghalaya

    • Authors: K. MAHENDRA; P. BAISWAR*, S. CHANDRA, B.U. CHOUDHURY, D. MAJUMDER T. RAJESH D.M. FIRAKE
      Abstract: Disease samples of sheath blight of rice, and banded leaf and sheath blight of maize were collected randomly from four districts of Meghalaya. Molecular characterization using specific primers and universal fungal primers, revealed that most of the isolates belonged to anastomosis group AG1-IA. Differential growth rate was observed in 15 rice isolates of R. solani with maximum growth rate observed in two rice isolates, KMR6 and KMR8 that covered whole petri plates within 2 days of inoculation. In case of 12 maize isolates, KMM7 covered whole plate after 36 hours of inoculation. Virulence analysis of 15 rice isolates by detached tiller method revealed that isolates KMR2, KMR3, KMR4, KMR5, KMR6, KMR7, KMR8, KMR11, KMR12, KMR13 and KMR15 were virulent and other isolates were non-virulent. While detached leaf assay of maize showed that the isolates KMM4, KMM6, KMM7 and KMM8 were virulent and other isolates were non-virulent. Thirty six soil samples were collected from across the Meghalaya state and analyzed for soil pH, soil organic carbon (SOC), soil texture and available nutrients (N, P and K). Correlation analysis revealed that growth of KMM7 isolate in soil was significantly correlated with sand%, SOC, pH and available phosphorus (kg/ha) in soil. By using Step wise regression analysis, soil pH was detected as the most important variable. Among all equations evaluated (quadratic, inverse, logarithmic and linear), quadratic (adjusted R2 - 0.52) and linear (adjusted R2 - 0.45) were found to explain the variability better than other equations.
      Issue No: Vol. 69, No. 3
       
  • Diversity of foliicolous fungi on Peach (Prunus persica) from Kashmir
           valley of India

    • Authors: NADIA ASHRAF; MOHD. YAQUB BHAT*, A.H. WANI, JAHINGIR A. KOKA SHAZIA PARVEEN
      Abstract: Peach (Prunus persica (L.) Batsch) is one of the most important and popular stone fruit of Kashmir Valley. Peach crop and yield is reduced every year due to number of foliicolous fungi. Extensive works on foliicolous fungi have not been carried out in Kashmir Valley. Therefore, present work was carried out to isolate and identify thee foliicolous fungi infecting peach plants from Kashmir Valley. It was revealed from the present study that six species of foliicolous fungi infect different varieties of peach (Prunus persica L.) plants in Kashmir Valley. These foliicolous fungi were Alternaria alternata, Wilsonomyces carpophilus, Cladosporium carpophilum, Entomosporium maculatum, Monilinia fructicola and Trichothecium roseum respectively. Most of these foliicolous fungi were reported first time from the Kashmir Valley.
      Issue No: Vol. 69, No. 3
       
  • Evaluation of post-symptom activities of fungicides against Marssonina
           coronaria causing premature leaf fall in apple

    • Authors: A. KUMAR*; J.N. SHARMA
      Abstract: Experiments were conducted to evaluate the effect of post-symptomatic fungicide application on conidial production (Cp), germinability (Cg) and probable number of conidial production/lesion (PNVC) of Marssonina blotch of apple caused by Marssonina coronaria. Fungicides from seven chemical classes were evaluated for their effect on Cg, Cp and PNVC on 1st, 3rd, 5th, 7th, 9th and 14th day after spray. Two sprays were given under each treatment at 7 days interval. All the fungicides significantly reduced Cg, Cp and PNVC with control at all the times of observation. Inhibitory effect on Cp, Cg and PNVC was recorded highest on the first day of observation after each spray thereafter, gradual decrease was recorded. It was observed that under higher disease pressure this pathogen requires repeated applications of fungicides to bring down the level of PNVC for further spread of the disease. All the treatments inhibited Cp and Cg to a grater extent after second spray as compared to after their first application. The quinine outside inhibitor (QoI) fungicide (kresoxim methyl) exhibited significantly lowest Cp, Cg and PNVC over control with no phytotoxicity on apple leaves at used concentrations (0.025, 0.05 and 0.1%). Strobilurins were closely followed by benzimidazoles and dodine. Triazoles showed average results whereas, dithiocarbamates, shield, copper oxychloride and captan showed poor results in this respect. Strobilurin fungicide kresoxim methyl (0.1%) found most effective exhibited least average PNVC (0.03-0.12x104 conidia ml-1) of M. coronaria whereas, control registered highest (17.01x104 conidia ml-1). Therefore, strobilurins can be recommended for the effective management of Marssonina blotch of apple.
      Issue No: Vol. 69, No. 3
       
  • Chemical composition and fungitoxic activity of essential oils of
           Chromolaena adenophorum Spreng, Ageratum conyzoides L. and Lantana camara
           L.

    • Authors: RAJAN KATOCH*; NEELAM THAKUR
      Abstract: The essential oils of Ageratum conyzoides L., Lantana camara L. and Chromolaena adenophorum Spreng were extracted and analyzed by GC-MS. The essential oil composition of the aerial parts of three plants was mainly dominated by sesquiterpenes. The principal constituents of aerial parts of Ageratum identified were Precocene I (28.84%). In Lantana Nerolidol (22.02%) and cis-caryophyllene (9.06%) were major essential oils. The essential oil from aerial parts of Chromolaena was mainly dominated by 1-phellandrene (11.73%) and torreyol (11.41%). The antifungal activity of essential oils was examined by disc diffusion method against six phytopathogenic fungi viz., Rhizoctonia solani, Sclerotium rolfsii, Fusarium solani, Fusarium oxysporum, Alternaria brassicaceae and Phoma medicaginis. Ageratum essential oil was highly effective against fungal growth, followed by Lantana and Chromolaena. The Ageratum essential oil was most effective against the growth of S. rolfsii, followed by R. solani, F. oxysporum, F. solani, A. brassicaceae and P. medicaginis. Chromolaena essential oil showed broadest antifungal spectrum against F. oxysporum, followed by A. brassicaceae, R. solani, F. solani and P. medicaginis. The Lantana essential oil exhibited no antifungal activity against P. medicaginis. MICs of the essential oils were also determined. The essential oil of Chromolaena was effective against F. oxysporum and A. brassicaceae; Ageratum against R. solani, S. rolfsii, F. solani, F. oxysporum and Lantana against S. rolfsii, F. solani, F. oxysporum and A. brassicaceae even at very low concentration (10%). The fungitoxic effects of the essential oils revealed future possibility of their use in plant protection strategies.
      Issue No: Vol. 69, No. 3
       
  • Molecular mapping of QTLs for Karnal bunt resistance in six near isogenic
           (NILs) populations of bread wheat

    • Authors: RITU BALA*; A. SHARMA, I. SHARMA, N.S. BAINS, LENIKA KASHYAP H. BARIANA
      Abstract: Karnal bunt (KB) of wheat, caused by the fungus Tilletia indica, is a challenge to the grain industry, owing not to direct yield loss but to quarantine regulations that may restrict international movement of affected grain. For mapping quantitative trait loci (QTLs) for Karnal bunt in wheat (Triticum aestivum L.) a susceptible cultivar WH542, was crossed with Karnal bunt resistant stocks to construct the NIL mapping populations. Six BC5F1 NIL populations were screened for Karnal bunt using 625 simple sequence repeat markers spanning the whole genomic region. Our total survey of markers showed the presence of the introgressed segment which could be associated with Karnal bunt resistance on chromosomes regions 1A, 1D, 2B, 2D, 3A, 4A, 4B, 5A, 5D, 6A, 6B, 7A, 7B and 7D. The study postulated new regions associated with KB resistance.
      Issue No: Vol. 69, No. 3
       
  • Screening of soybean germplasm for resistance to damping-off caused by
           Pythium ultimum

    • Authors: BHAGYASHALI V. HUDGE*; PRAMOD P. DESAI G.D. DESHPANDE
      Abstract: Hundred soybean germplasm lines were screened for resistance against Pythium ultimum Trow. Results showed that out of one hundred germplasm lines only eight entries viz., MAUS-102, JS-98-2, Kalitur, Himso-1587, MAUS-47, MAUS-71, MAUS-158 and MAUS-162 have expressed resistance towards pre-emergence damping-off disease. Also, in post-emergence mortality studies, the resistance were classified into 5 categories on the basis of seedling rot percentage i.e., resistant (8), moderately resistant (8), moderately susceptible (7), susceptible (16) and highly susceptible (61). This resistance grading can further be utilized to understand the resistance mechanism in soybean germplasm as well as in soybean breeding evaluation program.
      Issue No: Vol. 69, No. 3
       
  • Role of Meloidogyne javanica on severity of vascular bacterial wilt of
           eggplant

    • Authors: P.P. GHOSH; S. DUTTA*, MATIYAR RAHAMAN KHAN A. CHATTOPADYAY
      Abstract: The present investigation was aimed to know the effect of time and order of inoculation of Meloidogyne javanica and Ralstonia solanacearum on bacterial wilt disease severity and plant growth of eggplant. Results showed that M. javanica caused reduction in shoot length (39%). Concomitant presence of both pathogens before transplanting resulted into lowest shoot fresh weight (41.70g). Significantly higher root fresh weight (16.75g) was recorded due to higher number of gall (925.67) and eggmass (771.66) per root system in sole inoculation of M. javanica prior to R. solanacearum. Lower root galling and eggmass production was recorded when R. solanacearum inoculated prior to and simultaneously with M. javanica (275 & 220.67; 390.67 & 248.67; 402 & 351.67; 453.33 & 274). Conclusively inoculation of M. javanica aggravated the disease severity (60-75%) than R. solanacearum alone (35.00%).
      Issue No: Vol. 69, No. 3
       
  • Evaluation of single spore isolates of Agaricus bisporus and their genetic
           characterization

    • Authors: POOJA KAPOOR*; ABHISHEK KATOCH, NAIYA SHARMA, PREM NATH SHARMA SUBHASH DHANCHOLIA
      Abstract: Single spore isolation is one of the appropriate methods for production of high yielding and quality mushroom strains. In button mushroom (Agaricus bisporus) 500 single spore isolates of U3 and A15 parents were raised for getting their better productivity and quality. Among 500 single spore isolates raised in present study, 30 (20 from U3 and 10 from A15) were selected on the basis of fast and strandy mycelial growth (>2.0 mm per day) and their colony morphology. Out of 30 tested isolates, U3-1, U3-8 and U3-7 strains were identified as high yielding strains having 22.3, 21.9 and 20.7 kg/100 kg compost respectively as compared to 18.10 kg/100 kg compost in parent U3 strain within six weeks harvesting period. Similarly, isolate A15-5 of parent A-15 was recorded as best yielder strain having 14.95 kg/100kg compost as compared to 9.50 kg/100kg compost obtained by parent A-15 strain within 6 week harvesting period. RAPD studies shown that the percentage of polymorphism detected was 56.9 per cent and maximum number of bands was produced by OPD-12 primer among high yielding single spore isolates. Based on 73 alleles amplified by RAPD markers a dendrogram was generated using NTYSc 2.1 software that clustered high yielding single spore isolates into six variants at 73 per cent similarity coefficient.
      Issue No: Vol. 69, No. 3
       
  • Exploring broad spectrum antibiotic genes of Fluorescent Pseudomonads in
           sustainable crop disease management

    • Authors: M.K. NAIK
      Abstract: At the outset, I feel privileged to present Prof. A.K. Sarbhoy Memorial Award Lecture to this august gathering of plant pathologists. I also thank the Indian Phytopathological Society for the honour bestowed upon me to deliver this prestigious award lecture. Late Dr. A.K. Sarbhoy, a renowned mycologist and a distinguished teacher with great commitments, who taught the courses such as mycology and physiology of fungi during our days at IARI. I pray rich tributes to the great soul on this occasion. I have chosen a topic on exploring broad spectrum antibiotic genes of fluorescent pseudomonads in sustainable crop disease management, which is very much relevant to the current needs in plant pathology.
      Issue No: Vol. 69, No. 3
       
  • Race profiling, genetic diversity, diagnostics and management of Fusarium
           oxysporum f. sp. ciceris causing wilt in chickpea

    • Authors: S.C. DUBEY
      Abstract: Fusarium oxysporum f. sp. ciceris (Foc) is a soil and seed- borne fungal pathogen causing chickpea wilt worldwide and produces typical vascular wilt symptoms in India. The populations were highly variable in their growth pattern and rate, size of macroconida and pigmentation in culture. The studies clearly indicate the need to revise the International differential cultivars standardized in 1982 for characterization of the Indian populations of Foc into races. A set of new differential cultivars namely, C104, JG74, CPS1, BG212, WR315, KWR108, GPF2, DCP92-3, Chaffa and JG62 was identified and based on differential reactions; the Indian populations of Foc were characterized into eight races. Genetic diversity analysis using various molecular markers such as RAPD, URP, ISSR and SSR indicated the existence of variability among the populations predominated by a single race of the pathogen in an area. The groups partially corresponded to the states of origin/chickpea-growing region of the isolates as well as races of the pathogen. Gene specific markers such as TEF-1α, β-tubulin, and ITS were also used to determine the genetic diversity and about 90% populations showed more or less similar grouping pattern with these markers. The groups did not correspond to the state of origin and races of the pathogen. High level of similarity among the populations in respect of these gene sequences was observed. Highly sensitive and specific molecular markers from various genomic regions as well as SCAR markers were developed for detection of the pathogen using both conventional and real-time PCR. For management of the disease, efficacy of various bio-agents was evaluated and Trichoderma harzianum and T. viride were found effective. Novel seed dressing and soil application formulations were developed from these bio-agents. A combination of PBP 4G (T. viride) for soil application and Pusa 5SD (T. harzianum) for seed treatment together with a fungicide, carboxin provided the highest seed germination, shoot and root lengths and grain yield with the lowest incidence of wilt in chickpea under field conditions. The efficacy of seed treatment with Pusa 5SD has been validated at different locations in India. Further, a combination of seeds treated with Pusa 5SD developed from T. harzianum + Pseudomonas fluorescens 80 + Mesorhizobium ciceri + Vitavax power also provided the highest germination, grain yield and the lowest wilt incidence.
      Issue No: Vol. 69, No. 3
       
 
 
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