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Publisher: Indian Council of Agricultural Research   (Total: 6 journals)   [Sort by followers]

Fishery Technology     Open Access   (Followers: 2)
Indian Horticulture     Full-text available via subscription   (Followers: 9)
Indian J. of Agricultural Sciences     Open Access   (Followers: 5, SJR: 0.276, h-index: 15)
Indian J. of Animal Sciences     Open Access   (Followers: 5, SJR: 0.23, h-index: 14)
Indian J. of Fisheries     Open Access   (Followers: 2, SJR: 0.222, h-index: 4)
Indian Phytopathology     Open Access   (Followers: 2)
Journal Cover Indian Phytopathology
  [2 followers]  Follow
    
  This is an Open Access Journal Open Access journal
   ISSN (Print) 0367-973X - ISSN (Online) 2248-9800
   Published by Indian Council of Agricultural Research Homepage  [6 journals]
  • REP-PCR fingerprinting based genetic variability in Tilletia Indica

    • Authors: M. KAUR; R. SINGH*, M.S. SAHARAN, R.P. MANDHAN, R. BALA I. SHARMA
      Abstract: The repetitive sequence based polymerase chain reaction (rep-PCR) was evaluated as a tool to investigate variability among different isolates of Tilletia indica. DNA primers (BOX and ERIC) corresponding to conserved repetitive element motifs, originally described in prokaryotes, were used to generate genomic fingerprints of 18 isolates of T. indica from different locations of North Western Plain Zone of India. The amplified bands in BOX ranged in length from 600 to 3200 bp whereas for the ERIC, bands ranged from 400 to 3000 bp. A total of 72 bands were amplified of which 70 (97.2%) were polymorphic. The cluster analysis showed that most isolates from the same regions were grouped in the same cluster or a close cluster. However, the isolate, KBJ2 from Jammu was found to be the most distinct from rest of the isolates. The variability found within closely related isolates of T. indica demonstrated the effectiveness of rep-PCR marker in identifying genetic diversity among T. indica isolates. The results of Principal Component Analysis (PCA) were similar to those detected by cluster analysis. The first two major axis of differentiation (PC1 and PC2) explained 52.98% of the total variation.
      Issue No: Vol. 68, No. 4
       
  • Confirmation of resistance in wheat germplasms against brown/leaf rust
           through seedling resistance test (SRT) and adult plant reaction (APR)

    • Authors: ELANGBAM PREMABATI DEVI; DEEPSHIKHA* BIMLA KUMARI
      Abstract: Identification of slow rusting resistance genes against leaf rust is important for gene pyramiding, gene deployment and developing slow-rusting wheat cultivars to control the disease. Nine hundred and eighty germplasm accessions were screened for their infection response against leaf rust during 2012-2013 season in the field condition and out of which 30 accessions which showed resistance response were selected for seedling resistance test (SRT) and adult plant resistance (APR) during 2013-2014 season. At seedling stage, three accessions viz. EC-635627, EC-635721 and EC-664244 showed resistance which may be the presence of seedling resistant Lr genes. At adult stage, the accessions EC-635627, EC-635705, EC-635721 and EC-664244 showed high resistance to leaf rust. Except the accession EC-635614, remaining twentynine germplasm accessions showed adult plant resistance phenotypically as shown by their lower A-values which may be the presence of effective race-specific resistance genes. The relation between the plot yield and A-value of 30 germplasm accessions was observed to be negatively correlated.
      Issue No: Vol. 68, No. 4
       
  • Characterizing diversity of Dickeya zeae causing bacterial stalk rot of
           maize based on biochemical assays and antibiotic sensitivity

    • Authors: ADESH KUMAR; MANDEEP SINGH HUNJAN*, HARLEEN KAUR P.P. SINGH
      Abstract: Maize growing areas of Punjab were surveyed during Kharif 2012 and 2013 for the occurrence of stalk rot disease. A total of sixty two isolates were collected, isolated and identified as off white colonies appearing on King’s B medium. The isolates showed variation in virulence when tested on susceptible cv. Punjab Sweet Corn-1 and were grouped as highly virulent, moderately virulent and less virulent. All the isolates produced cavities on crystal violet pectate medium. A panel of 27 biochemical tests were used to characterize these isolates which showed differential reaction for utilization of starch and other carbohydrates, gelatin liquefication and growth at high salt concentration. Multiple antibiotic resistance was observed in all the isolates tested. Numerical analysis of phenotypic features revealed two major groups of isolates associated at 60 per cent similarity coefficient.
      Issue No: Vol. 68, No. 4
       
  • Characterization and genetic diversity of Ralstonia solanacearum causing
           brown rot disease of potato

    • Authors: R.K. RANJAN; DINESH SINGH*, P. SHARMA SHRI DHAR
      Abstract: The genetic diversity among Indian strains of Ralstonia solanacearum, causal agent of bacterial wilt of potato, was assessed by repetitive sequence-based polymerase chain reaction (rep- PCR) method. Sixty three strains of R. solanacearum isolated from potato, which were collected from different states of India viz. Uttarakhand, Meghalaya, West Bengal, Himachal Pradesh, Orissa, and Karnataka under different agro-climatic regions. Characterization of races and biovars of these strains of R. solanacearum was done and two races (race 1 and race 3) and four biovars (bv 1, bv 2, bv2T and bv3) were recorded from potato. Genetic diversity these 63 strains of R. solanacearum was done by using rep-PCR (BOX, ERIC and REP- PCR primers). The strains of R. solanacearum formed 42 clusters (group) at 70% similarity coefficient. The strains of R. solanacearum isolated from Uttarakhand formed group 1- 5, 8-9 and group 23, from Meghalaya formed groups 6-7, 10-20, 24, 38-39, from West Bengal formed groups 25 and 27, from Odisha formed groups 26, 35- 37 and 40- 42, from Karnataka formed groups 22, 30-34 and from Himachal Pradesh formed group 21, 28-29. Maximum genetic diversity in strains of R. solanacearum was found, which were collected from Shilong (Meghalaya) and clustered in 18 clusters followed by strains collected from Nainital, hill areas (Uttarakhand) divided into 8 groups. However, the strains collected from plains i.e. Karanataka belongs to R1/bv3 cluster in one group, likewise the strains of Odisha R3/bv2 & 2T clustered in separate group and strains from West Bengal R1/bv3 clustered in one group. Thus the study indicated the existence of wide range of genetic diversity among the Indian strains of R. solanacearum causing brown rot of potato.
      Issue No: Vol. 68, No. 4
       
  • Disease reaction of onion genotypes to Alternaria porri and stability of
           resistance and yield

    • Authors: SRUJANI BEHERA; HIJAM MERONBALA DEVI, SUNITA MAHAPATRA* SRIKANTA DAS
      Issue No: Vol. 68, No. 4
       
  • First report of Alternaria alternata causing leaf spot disease of
           Alstroemeria hybrida

    • Authors: KISHOR SHARMA; H.R. GAUTAM NARENDER K. BHARAT*
      Issue No: Vol. 68, No. 4
       
  • Evaluation of systemic acquired resistance (SAR) activators for the
           management of leaf blotch of apple caused by Marssonina coronaria

    • Authors: S. PHURAILATPAM*; J.N. SHARMA
      Issue No: Vol. 68, No. 4
       
  • Eryngium foetidum (wild coriander): A new host record of powdery mildew
           pathogen (Oidiopsis macrospora) from India

    • Authors: A.K. RATANKUMAR SINGH*; S.K. DUTTA, S.B. SINGH L. SANAJAOBA SINGH
      Issue No: Vol. 68, No. 4
       
  • Identification of Phytoplasma associated with weed species in India

    • Authors: SMRITI MALL; SHAILENDER KUMAR, VIKAS JADON G.P. RAO*
      Issue No: Vol. 68, No. 4
       
  • Incidence and detection of Potato virus Y on potato crop in Jorhat
           District of Assam by DAS-ELISA

    • Authors: RANIMA MISHRA*; P.D. NATH
      Issue No: Vol. 68, No. 4
       
  • Isolation and Characterization of Partial secA Gene from Coconut Root Wilt
           and Arecanut Yellow Leaf Disease Phytoplasma and assessment of its
           possible use for sero diagnostics

    • Authors: R. MANIMEKALAI*; SMITA NAIR, K.P. GANGARAJ, V.P. SOUMYA G.P. RAO
      Abstract: A 425 bp fragment of phytoplasma secA gene encoding SecA, a component of the Sec protein translocation system, was isolated and characterized from coconut root wilt disease and arecanut yellow leaf disease phytoplasma, both belonging to the 16SrXI-B sub group phytoplasma. The gene showed 99% nucleotide identity with sugarcane grassy shoot phytoplasma secA gene. In the phylogenetic analysis of the translated SecA protein sequence, all the 16SrXI group phytoplasmas clustered together.  The three dimensional structure of the protein was predicted using Moddler 9.13. The validation of the predicted structure indicated that 83.6% of the amino acids lie in the favored region indicating that the predicted structure was ideal. The possible antigenic domains in the protein were also predicted using the tool SVM Trip. The antigenicity prediction of the SecA protein fragment showed the presence of 4 possible antigenic domains. The secA gene was expressed in pET vector and the protein was used for the development of polyclonal antiserum in rabbit. Because the titre of the phytoplasma was very less, the color formation in ELISA was less intense. A technique for isolation of higher number of phytoplasma cells has to be standardized.
      Issue No: Vol. 68, No. 4
       
  • Molecular identification of Narcissus yellow stripe virus strain
           associated with severe mosaic disease of Narcissus from India

    • Authors: NEELAM YADAV*; JAWAID A. KHAN
      Abstract: A Potyvirus associated with severe mosaic disease of Narcissus tazetta in India was characterized at molecular level. Nucleotide sequence of its 3΄-terminal genome (~2900 bp) comprising 3΄-non translated region (NTR), large nuclear inclusion protein (NIb, RdRp, replicase), coat protein (CP) and C-terminus of NIa-proteinase (NIa-Pro) showed 93% and 85% homology whereas, CP gene showed 97% and 87% identities with Narcissus yellow stripe virus (NYSV) at amino acid and nucleotide level, respectively. 3΄-NTR showed maximum homology of 98% with NYSV.  Phylogenetic analyses of present viral genome showed its close relatedness to NYSV, at nucleotide and amino acid level using CP, NIb and 3΄-NTR. Thus, it can be clearly concluded that it is a new strain of Narcissus yellow stripe virus from India.
      Issue No: Vol. 68, No. 4
       
  • Survival of Fusarium moniliforme causing foot rot and bakanae disease in
           different parts of rice grains

    • Authors: PANKAJ KUMAR; S. SUNDER RAM SINGH*
      Issue No: Vol. 68, No. 4
       
  • Evaluation of fungicides and a bio-control agent against powdery mildew
           disease of apple cv. Ambri

    • Authors: T.R. RATHER*; Z.A. BHAT N.A. KHAN
      Issue No: Vol. 68, No. 4
       
  • Comparative analysis of 16S/23S intergenic region of Candidatus
           Liberibacter asiaticus associated with citrus greening disease in
           different citrus cultivars of Assam

    • Authors: SUMIT BHOSE; PRAGATI MISRA, PRADEEP KUMAR DILIP GHOSH*
      Abstract: Citrus greening, also known as Huanglongbing (HLB) is one of the most destructive diseases to commercial citrus plantations in India. Among the three different species of the causal bacterium reported worldwide, Candidatus Liberibacter asiaticus is the only species prevalent with HLB throughout the country. Though HLB has long been observed in different citrus cultivars and insect vectors (citrus psylla, Diaphorina citri Kuwayama) from North eastern territory of India, knowledge on the molecular variability in ' Ca. L. asiaticus' populations from this geographical region is limited. In this study, HLB was detected and identified by symptomatology, biological indexing on indicator plants and PCR (polymerase chain reaction) with 'Ca. L. asiaticus' specific 16S rDNA primers. Comparative analysis of 16S/23S intergenic region sequences of six representative 'Ca. L. asiaticus' isolates infecting three different citrus cultivars in Assam viz. Khasi  mandarin (Citrus reticulata), Assam lemon (Citrus limon)  and  Sweet orange (Citrus sinensis) revealed  that  sequences were 99-100% similar to the published sequences of  'Ca. L. asiaticus'  from Brazil, Taiwan, USA and some Indian (Poona) strains. However, approximately 88% sequence identity was seen with an Africanas strain (JF819885). Phylogenetic analysis with various published Candidatus Liberibacter species sequences revealed that all asiaticus isolates remained in one group while AsL-i isolate was quite distinct from other strains and close to a Chinese strain of the pathogen. The data also reveals that the genomic region can be used for HLB detection but not for strain differentiations.
      Issue No: Vol. 68, No. 4
       
  • Comparative economics of spray scheduling for management of late leaf spot
           of groundnut (Arachis hypogaea) incited by Phaeoisariopsis personata

    • Authors: UTPAL DEY*; D.N. DHUTRAJ, A.P. SURYAWANSHI, ANUP DAS S.P. DAS
      Abstract: Late leaf spot (LLS), caused by Phaeoisariopsis personata, a foliar disease of groundnut or peanut (Arachis hypogaea) is one of the important factors limiting groundnut productivity in India. It frequently inflicts colossal yield losses of more than 50% on unsprayed peanuts. The current studies emphasized upon the management strategies to mitigate the losses caused by the disease. Among the fungicides, Difenoconazole (@ 0.1%) recorded highest pod yield (1757 kg/ha) and test weight (30.75 g) with least disease incidence (17.98%), severity (12.30%), minimum defoliation (4.83%),  minimum frequency (1.20/mm2) and reduced size (1.31 mm2) and thereby increased the pod yield by 53.04 per cent and test weight by 21.79 per cent over unsprayed control followed by Propiconazole (@ 0.1%) which recorded pod yield of 1649 kg/ha and test weight of 29.90 g with disease incidence (19.53%), severity (16.58%) and minimum defoliation (5.66%) and thereby increased the pod yield by 49.97 per cent and test weight by 19.57 per cent over unsprayed control. Both the botanicals (Mehandi @ 10% and NSKE @ 5%) and bioagents (Trichoderma viride, Verticillium lecanii and Bacillus circulans @ 5%) recorded significantly higher pod yield (range, 1089 - 1333 kg/ha) and test weight (range, 24.39 - 26.88 g) and increased the pod yield (range, 21.88-38.11%) and test weight (range, 1.39-10.53%) over unsprayed control (yield 825 kg/ha and test weight 24.05 g). The late leaf spot disease incidence and defoliation were found to be reduced with increased number of (two-three) sprayings of the fungicides.
      Issue No: Vol. 68, No. 4
       
  • Screening of grape genotypes to identify sources of resistance to
           anthracnose disease and identifying biochemical marker associated with
           resistance

    • Authors: DINESH S. SHETTY; INDU S. SAWANT*, SHUBHANGI P. NARKAR, SHASHIKANT GHULE, J. SATISHA G.S. KARIBASAPPA
      Abstract: Two hundred and sixty-six grape genotypes maintained at National Active Germplasm Site at ICAR-National Research Centre for Grapes, Pune, were screened for their reaction against anthracnose disease. Among them, sixty one genotypes were identified as resistant sources for anthracnose disease. All major commercial cultivars were found susceptible. The levels of biochemical constituents in the leaves of 10 resistant and 10 susceptible genotypes were examined for their possible association with disease resistance which can be used as a biochemical marker. Before challenge inoculation with the pathogen the levels of total phenols (21.32 mg/g), flavonols (10.93 mg/g), and peroxidase activity (0.226 U/ml) were significantly higher in anthracnose resistant genotypes. After challenge inoculation with the pathogen, apart from total phenols (25.91 mg/g), flavonols (13.35 mg/g), and peroxidase activity (0.371 U/ml), the resistant genotypes also showed higher levels of flavonoids (9.55 mg/g), flavon-3-ols (1.94 mg/g) and polyphenol oxidase activity (0.0471 U/ml), but the percent increase data was significant only for total phenols, flavonoids, polyphenol oxidase and peroxidase. Five of the selected anthracnose resistant (or susceptible) genotypes were also resistant to downy mildew disease while, the other five were susceptible to downy mildew, thus, classifying the 20 genotypes into 4 groups. Interestingly, only peroxidase activity was significantly higher in the 2 anthracnose resistant groups as compared to the 2 susceptible groups. Furthermore, cluster analysis confirmed that, peroxidase activity was the only clear indicator of anthracnose resistance and can be used as a biochemical marker.
      Issue No: Vol. 68, No. 4
       
  • Botanical, chemical and biological management of Stemphylium botryosum
           blight disease of lentil in Nepal

    • Authors: SUBASH SUBEDI*; SUNDAR MAN SHRESTHA, K.C. GOPAL BAHADUR, RESHAM BAHADUR THAPA, SURYA KANTA GHIMIRE, SARASWATI NEUPANE BADRUN NESSA
      Abstract: Integrated disease management approach was attempted for lentil stemphylium blight caused by Stemphylium botryosum Walr during 2011-12 and 2012-13 in vitro, in vivo and also screened for host resistance genotypes. Over years, botanicals Acorus calamus L. and Xanthozylum armatum DC, fungicides Mancozeb and Krilaxyl and antagonist Trichoderma viridae were effective for disease control and yield increment. Extract of A. calamus at higher dose (8% W/V) and Krilaxyl even at lower (500 ppm) checked the pathogen growth completely in-vitro. On 5th day of incubation period, mycelium growth of the pathogen was collapsed due to fungal antagonist T. viridae. Mycelial growth inhibition percent of X. armatum (8% W/V) and Mancozeb (2000 ppm) on PDA was 31.17 and 55.94, respectively. In field, botanicals were sparingly effective for a short period. Percent disease control (PDC) was higher in A. calamaus (46.60%) and X. armatum (46.26%) compared to unsprayed plot. Higher percent yield increase (PYI) was obtained from Krilaxyl (71.22%) and Mancozeb (55.31%) @ 2 g/l of water over control. The lower Percent Disease Index (PDI) was observed in Krilaxyl (36.00) and Mancozeb (37.35). The PDC and PYI were higher in T. viridae (PPD isolate) i.e. 42.14% and 58.80% respectively. Out of 58 genotypes screened, RL-28, ILL 10134, RL-44, FLIP 2008-7L, NR-2001-71-3, NR-2001-71-4 and ILL 7657 were found resistant to the disease.
      Issue No: Vol. 68, No. 4
       
  • Integrated management of pea diseases through seed-cum-soil treatment with
           bioagents and fungicide in dry temperate zone of North Western Himalaya

    • Authors: PARDEEP KUMAR*
      Abstract: Root rot/wilt complex (Fusarium oxysporum f. sp. pisi, F. solani f. sp. pisi and Rhizoctonia solani) was found widely distributed in moderate to high intensity whereas white rot (Sclerotinia sclerotiorum) was in low to moderate proportion during survey in 2006-2013 Kharif cropping season in dry temperate zone of North Western Himalaya. Eight years average disease incidence of root rot and white rot was 34.0% and 8.4% respectively. The efficacy of Trichoderma viride, Pseudomonas fluorescens and carboxin 75WP (vitavax) were evaluated as seed dressing and soil application individually and in combinations under field experiments during Kharif 2012-2013 for the management of Root rot/wilt complex and white rot of garden pea. Seed treatment with P. fluorescence + carboxin 75 WP at 2.5g/kg of seed was found significantly superior to other treatments in reducing root rot wilt incidence of 62.0% and 65.7% of white rot with highest yield potential of 89.8 q/ha. T. viride at 8g when combined with carboxin 75 WP at 2.5g/kg of seed resulted 19.8% and 3.0% root rot and white rot incidence, respectively as compared to 50.8% and 9.9% disease incidence in inoculated control. This study revealed that both soil application and seed treatment of T. viride and P. fluorescens has good potential in controlling diseases of pea.
      Issue No: Vol. 68, No. 4
       
  • Virulence diversity in Rhizoctonia solani isolates of different maize
           growing ecosystems of India

    • Authors: BHEEMARAYA*; ROBIN GOGOI, RASHMI AGGARWAL, A. KUMAR S.N. RAI
      Abstract: Fifty eight Rhizoctonia solani isolates collected from maize (43), rice (12), moong (2) and pigeon pea (1) were inoculated on to 35 days old susceptible maize cv. Vivek QPM-9 by placing two to three barley grain inoculum between stalk and sheath at second or third inter-node level. The virulence diversity among the isolates revealed that all the isolates could cause infection on maize susceptible cultivar irrespective of their hosts of origin. The time taken for R. solani isolates to express symptoms on maize crop varied from 1.0 to 3.4 days. The percent disease index (PDI) ranged from 56.0 to 90.0%. The virulence index varied from 16.5 to 90.0. The length of infected area recorded in different R. solani isolates ranged from 50.2 to 85.6 cm. The relative length of infected area produced by different isolates varied from 26.7 to 45.5%. Among all isolates, two maize isolates M25 and M16 of R. solani f. sp. sasakii were superior in all above mentioned parameters.
      Issue No: Vol. 68, No. 4
       
  • Bio-agent based integrated management strategy against stem rot for
           chickpea

    • Authors: A.K. MANDAL; SUNIL C. DUBEY* ARADHIKA TRIPATHI
      Abstract: Sclerotinia sclerotiorum is soil-inhabiting and considered one of the most devastating plant pathogens causing stem rot in chickpea. To develop an effective management strategy for the disease, various bio-agents and fungicides were evaluated against the pathogen.   Trichoderma viride showed superiority among the various bio-agents evaluated in vitro against S. sclerotiorum. Out of 5 fungicides evaluated against S. sclerotiorum and T. viride, iprodion caused 100% growth inhibition for the both, while carboxin was less inhibitory to T. viride than S. sclerotiorum. Therefore, carboxin was selected for seed treatment and iprodion for foliar spray in combination with T. viride based bio-formulation. A combination of soil application formulation Pusa Biopellet 4G (T. viride), seed treatment with Pusa 5SD (T. viride) + carboxin + Mesorhizobium ciceri and foliar spray with iprodion at 40 days after sowing provided the highest disease reduction and enhanced the plant health attributing parameters such as seed germination, shoot and root lengths and number of pods plant-1. Pusa Biopellet 4G and Pusa 5SD individually were also found effective in reducing disease incidence and improving plant health, but their combined application was found superior to the use of any of these formulations alone for all the variables.
      Issue No: Vol. 68, No. 4
       
  • Diversity and resistance analysis in wheat (Triticum aestivum) genotypes
           against spot blotch (Bipolaris sorokiniana) disease

    • Authors: R.D. MEENA*; R. AGGARWAL, S. SINHA, N.K. MEENA, P. SRINIVAS, N.L. MEENA M.S. GURJAR
      Abstract: In an experiment twenty wheat and triticale genotypes including indigenous and exotic were evaluated three years (2007-08, 2008-09 and 2009-10) against different isolates i.e., BS 25, BS 34 and BS 75 spot blotch disease causing pathogen (B. sorokiniana). Seedlings of the wheat genotypes showed different degrees of Per cent Disease Index (PDI) with a range of 16.04 to 59.99. Maximum spot blotch severity was recorded on Lok Bharti (PDI 59.99) followed by in genotype Agra Local (PDI 56.7). Four wheat genotypes (Shisenzhary 86 (PDI 18.49), Synthetic-23 (PDI 16.04), Chuanmai-18 (PDI 19.02) and BVE x Atila (PDI 19.20) were showed moderately resistance whereas, one genotype (Chirya-3) was found moderately susceptible with PDI 29.83. URP profiles confirmed extremely high level of polymorphism/genetic variability (90%) among wheat genotypes. The URP dendrogram illustrated one major cluster of susceptible wheat genotypes and one minor cluster of four moderately resistant wheat genotypes (Shisenzhary 86, Synthetic 23, Chuanmai 18 and BVE x Atila). Principal component analysis performed on URP data explained 70.41% variation available among the wheat genotypes.
      Issue No: Vol. 68, No. 4
       
  • Introgression of bacterial blight resistance gene xa13 in rice cultivar
           Poornima through marker assisted breeding

    • Authors: KADU TANVI PRADIPRAO*; KALE SONAM SURESHRAO, GADAMBE SHILPA DNYNOBARAO, TOSHY AGRAWAL A.S. KOTASTHANE
      Abstract: In the present investigation, F3segregating population of rice, derived from IRBB13 x Poornima, was inoculated with a bacterial leaf blight (BLB) pathogen; Xanthomonas oryzae pv. Oryzae. Phenotypic screening of the progenies (F4population) was performed to select BLB resistant lines. Previously known microsatellite markers were used for the tagging of BLB resistant gene in those lines. Polymorphism and segregation patterns of the markers were studied by cleaved amplified polymorphic sequence analysis (CAPS). Lines no 3 and 33 were found to be resistant to BLB in phenotypic screening. Markers RM23483, RM2344, SR6, E6a and RG136 were polymorphic and co-segregated as in phenotypic screening. The SSR marker RM23495 and RM23496 was used as cleaved amplification polymorphism sequence (CAPS) marker for selective genotyping, but it did not co-segregate as in phenotypic screening.
      Issue No: Vol. 68, No. 4
       
  • Influence of stem and root vascular anatomy of eggplant germplasm on
           severity of vascular bacterial wilt disease

    • Authors: P.P. GHOSH; S. DUTTA*, T. SETH A. CHATTOPADHYAY
      Abstract: The present investigation was attempted to study the anatomy of eggplant root and stem to eloquent the mechanism of susceptibility/tolerance of twelve germplasm against vascular bacterial wilt (VBW). Cross sections of root and stem of various germplasm seedlings were made at the age of 25 days after sowing using simple light microscope followed by severity measurement in a sick plot using pair row planting system. The results clearly indicated that the higher number of large vessel (metaxylem) in comparison to small vessel (protoxylem) in stem and higher inner area of large vessel in roots negatively correlated with disease severity as observed in 09/BRBWRES2, 09/BRBWRES5, 10/BRBWRES5, Horti, Utsav and Arka Nidhi (cumulative PDI, 11.68 to 16.40%). Stepwise multiple regression analysis showed that the ratio of large and small vessels sqmm-1 in vascular area in stem was the single most important anatomical character governing susceptibility/tolerance of the germplasm under study against VBW disease of eggplant. The susceptibility increased with decrease in metaxylem to protoxylem ratio in stem. The present finding would be a useful marker, identified as early as seedling stage, for breeders to develop tolerant variety to combat this serious disease.
      Issue No: Vol. 68, No. 4
       
 
 
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