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International Microbiology
Journal Prestige (SJR): 0.431
Citation Impact (citeScore): 1
Number of Followers: 5  

  This is an Open Access Journal Open Access journal
ISSN (Print) 1139-6709 - ISSN (Online) 1618-1905
Published by Institut d'Estudis Catalans Homepage  [19 journals]
  • Julio R. Villanueva, microbiologist, researcher, and mentor of generations
           of scientists

    • Authors: César Nombela
      PubDate: 2018-03-26
      Issue No: Vol. 20 (2018)
  • Genetic diversity reflects geographical origin of Ralstonia solanacearum
           strains isolated from plant and water sources in Spain

    • Authors: Paola Caruso, Elena G. Biosca, Edson Bertolini, Ester Marco-Noales, María Teresa Gorris, Concetta Licciardello, María M. López
      Abstract: The characterization and intraspecific diversity of a collection of 45 Ralstonia solanacearum strains isolated in Spain from different sources and geographical origins is reported. To test the influence of the site and the host on strain diversity, phenotypic and genotypic analysis were performed by a polyphasic approach. Biochemical and metabolic profiles were compared. Serological relationship was evaluated by Indirect-ELISA using polyclonal and monoclonal antibodies. For genotypic analysis, hrpB and egl DNA sequence analysis, repetitive sequences (rep-PCR), amplified fragment length polymorphism (AFLP) profiles and macrorestriction with XbaI followed by pulsed field gel electrophoresis (PFGE) were performed.The biochemical and metabolic characterization, serological tests, rep-PCR typing and phylogenetic analysis showed that all analysed strains belonged to phylotype II sequevar 1 and shared homogeneous profiles. However, interesting differences among strains were found by AFLP and macrorestriction with XbaI followed by PFGE techniques, some profiles being related to the geographical origin of the strains. Diversity results obtained offer new insights into the biogeography of this quarantine organism and its possible sources and reservoirs in Spain and Mediterranean countries.
      Keywords: Bacterial wilt · potato · soil · PFGE · AFLP
      PubDate: 2018-03-26
      Issue No: Vol. 20 (2018)
  • Transmission and survival of carbapenem-resistant Acinetobacter baumannii
           outside hospital setting

    • Authors: Ana Kovacic, Martina Seruga Music, Svjetlana Dekic, Marija Tonkic, Anita Novak, Zana Rubic, Jasna Hrenovic, Ivana Goic-Barisic
      Abstract: Acinetobacter baumannii origin and its epidemiology is under a great concern worldwide since this microorganism has become a leading nosocomial pathogen of the 21th century among the "ESKAPE" group of microorganisms. The aim of the study was to monitor and explore the epidemiology of this important hospital pathogen in the second largest clinical university hospital in Croatia. The presence of A. baumannii in hospital wastewater, as a route for possible transmission outside of the hospital setting, as well as its survival in environmental conditions including seawater, was investigated. During the examination period, ten both carbapenem and multidrug-resistant isolates of A. baumannii were recovered from hospital wastewater and compared to the clinical isolates originating from the same monitoring period. Multiplex PCR confirmed that four wastewater isolates harboured blaOXA-23-like, while five wastewater isolates harboured blaOXA-40-like genes sharing 100% sequence identity with blaOXA-72 sequence described in the same hospital in 2009, confirming the presence of an endemic cluster. Survival of A. baumannii in natural seawater was examined during 50 days of monitoring and to the best of our knowledge, was performed for the first time.
      Keywords: Acinetobacter baumannii · hospital wastewater · transmission · seawater
      PubDate: 2018-03-26
      Issue No: Vol. 20 (2018)
  • Fate of Bacillus cereus within phagocytic cells

    • Authors: Ivanna S. Rolny, Silvia M. Racedo, Pablo F. Pérez
      Abstract: In this study we assessed the interaction of different strains of Bacillus cereus with murine peritoneal macrophages and cultured phagocytic cells (Raw 264.7 cells). Association, internalization, intracellular survival, routing of bacteria to different compartments and expression of MHCII were assessed in cells infected with different strains of B. cereus in vegetative form. Association values (adhering + internalized bacteria) and phagocytosis were higher for strain B10502 than those for strains 2 and M2. However, after 90 min interaction, intracellular survival was higher for strain 2 than for strains M2 and B10502. Acquisition of lysosomal markers by B. cereus containing vacuoles (BcCV), assessed by LAMP1 and Lysotracker labelling occurred shortly after internalization. The highest ratio of LAMP1(+)-BcCV was found for strain M2. This strain was able to survive longer than strain B10502 which routes to LAMP1 containing vacuoles to a lesser extent. In addition, strain M2 stimulated expression of MHCII by infected cells. Confocal analyses 60 or 90 min post-infection showed different percentages of co-localization of bacteria with Lysotracker. Results suggest strain-dependent interaction and intracellular killing of B. cereus by phagocytic cells. These findings could be relevant for the pathogenic potential of Bacillus cereus strains.
      Keywords: Bacillus cereus · LAMP1 · phagocytosis · virulence · endocytic pathway · intracellular trafficking
      PubDate: 2018-03-26
      Issue No: Vol. 20 (2018)
  • Developmental stages identified in the trophozoite of the free-living
           Alveolate flagellate Colpodella sp. (Apicomplexa)

    • Authors: Raghavendra Yadavalli, Tobili Y. Sam-Yellowe
      Abstract: In this study we performed light, immunofluorescent and transmission electron microscopy of Colpodella trophozoites to characterize trophozoite morphology and protein distribution. The use of Giemsa staining and antibodies to distinguish Colpodella life cycle stages has not been performed previously. Rhoptry and β-tubulin antibodies were used in immunofluorescent assays (IFA) to identify protein localization and distribution in the trophozoite stage of Colpodella (ATCC 50594). We report novel data identifying “doughnut-shaped” vesicles in the cytoplasm and apical end of Colpodella trophozoites reactive with antibodies specific to Plasmodium merozoite rhoptry proteins. Giemsa staining and immunofluorescent microscopy identified different developmental stages of Colpodella trophozoites, with the presence or absence of vesicles corresponding to maturity of the trophozoite. These data demonstrate for the first time evidence of rhoptry protein conservation between Plasmodium and Colpodella and provide further evidence that Colpodella trophozoites can be used as a heterologous model to investigate rhoptry biogenesis and function. Staining and antibody reactivity will facilitate phylogenetic, biochemical and molecular investigations of Colpodella sp. Developmental stages can be distinguished by Giemsa staining and antibody reactivity.
      Keywords: Colpodella · Rhoptries · Trichocysts · Apical complex · Plasmodium RhopH3
      PubDate: 2018-03-26
      Issue No: Vol. 20 (2018)
  • Overview of laboratory methods to diagnose Leptospirosis and to identify
           and to type leptospires

    • Authors: Aurélie Marquez, Zoheira Djelouadji, Virginie Lattard, Angéli Kodjo
      Abstract: Leptospirosis is a virulent zoonosis with a global distribution. Pathogenic spirochetes of the genus Leptospira are responsible for this disease, and the primary animal reservoirs are rodentvvvs. Direct and indirect contact with infected urine constitutes the main route of transmission. Renal failure and advanced abortions are frequently observed in animals affected by leptospirosis, causing serious problems for farms. In humans, there is a high rate of mortality (10 percent), and farmers and persons in contact with water are frequently exposed. However, vaccines and strict prevention measures confer protection against leptospirosis. Serological tests facilitate the detection and identification of leptospire strains. Such tests are based on specific surface antigen recognition and are used for clinical analyses. To determine which serovars circulate in the environment, leptospires must be typed. Molecular methods, such as restriction enzyme-based techniques and the sequencing of specific regions, permit serovar identification. Unfortunately, although there are numerous techniques, they are not very efficient, and thus, new methods must be developed. With the advent of genomic sequencing, a substantial amount of information regarding leptospire genomes is now available, facilitating the selection of regions to discriminate between strains. Typing is important for both epidemiologic purposes and clinical analyses.
      Keywords: Leptospirosis · zoonosis · methods · diagnosis
      PubDate: 2018-03-26
      Issue No: Vol. 20 (2018)
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